CN110448374A - A kind of isolated human body impedance detection circuit and detection method - Google Patents
A kind of isolated human body impedance detection circuit and detection method Download PDFInfo
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- CN110448374A CN110448374A CN201910820243.2A CN201910820243A CN110448374A CN 110448374 A CN110448374 A CN 110448374A CN 201910820243 A CN201910820243 A CN 201910820243A CN 110448374 A CN110448374 A CN 110448374A
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B18/00—Surgical instruments, devices or methods for transferring non-mechanical forms of energy to or from the body
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/05—Detecting, measuring or recording for diagnosis by means of electric currents or magnetic fields; Measuring using microwaves or radio waves
- A61B5/053—Measuring electrical impedance or conductance of a portion of the body
- A61B5/0538—Measuring electrical impedance or conductance of a portion of the body invasively, e.g. using a catheter
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/48—Other medical applications
- A61B5/4836—Diagnosis combined with treatment in closed-loop systems or methods
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B18/00—Surgical instruments, devices or methods for transferring non-mechanical forms of energy to or from the body
- A61B2018/00571—Surgical instruments, devices or methods for transferring non-mechanical forms of energy to or from the body for achieving a particular surgical effect
- A61B2018/00577—Ablation
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- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B18/00—Surgical instruments, devices or methods for transferring non-mechanical forms of energy to or from the body
- A61B2018/00571—Surgical instruments, devices or methods for transferring non-mechanical forms of energy to or from the body for achieving a particular surgical effect
- A61B2018/00613—Irreversible electroporation
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B18/00—Surgical instruments, devices or methods for transferring non-mechanical forms of energy to or from the body
- A61B2018/00636—Sensing and controlling the application of energy
- A61B2018/00773—Sensed parameters
- A61B2018/00827—Current
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- A—HUMAN NECESSITIES
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- A61B18/00—Surgical instruments, devices or methods for transferring non-mechanical forms of energy to or from the body
- A61B2018/00636—Sensing and controlling the application of energy
- A61B2018/00773—Sensed parameters
- A61B2018/00875—Resistance or impedance
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- A61B18/00—Surgical instruments, devices or methods for transferring non-mechanical forms of energy to or from the body
- A61B2018/00636—Sensing and controlling the application of energy
- A61B2018/00773—Sensed parameters
- A61B2018/00892—Voltage
Abstract
The invention discloses a kind of isolated human body impedance detection circuit and detection methods, including impulse generator, data processing unit, data display unit, one or more therapeutic electrodes and a channel or two impedance collection units, the output end of the impulse generator and the input terminal of therapeutic electrode connect, the acquisition input terminal of impedance collection unit is also connect with the output end of therapeutic electrode respectively, the acquisition output end of impedance collection unit is connect with the input terminal of the data processing unit, also disclose its detection method, the test point of human body is contacted by one or more voltage detecting electrodes and current detection electrode, obtain voltage triggering signal and current trigger signal；After voltage triggering signal and current trigger signal are pre-processed respectively, it is sent into data processing unit and is calculated.The present invention can be carried out human body impedance detection, guarantees electroporation effect, can play real-time monitoring ablation effect, reduce the volume and weight of detection device circuit.
Description
Technical field
The invention belongs to human body impedance measuring technical fields, and in particular to a kind of isolated human body impedance detection circuit and inspection
Survey method.
Background technique
Tumour ablation treating technology refers under image guidance, by the energy levels such as radio frequency, microwave, freezing, laser, electric field essence
Standard is punctured to tumor target, implements precisely minimally invasive ablation, hatching in situ tumour is completely new accurate Minimally Invasive Surgery.Heating ablation
By being heated or being freezed to tissue, to destroy tumour cell.The physics ablation techniques of wide clinical application include argon at present
Helium knife cryoablation, radio frequency, microwave and laser heating ablation etc..These ablation techniques are although curative for effect, advantages, master
It wants the disadvantage is that be non-selectivity to disorganization, i.e., in zone of ablation in addition to tumor tissues, normal histoorgan such as blood
Pipe, nerve, bile duct, ductus pancreaticus etc. are totally corrupted.This is the disadvantage is that cause the main reason for melting Post-operative complications, such as
Clinical common ablation post-operative hemorrhage volume, biliary system damage, intestinal tube perforation and neurological dysfunction etc..This nothing of heating ablation
Selective disorganization greatly increases the clinical application risk of technique, and seriously limits it in clinical application range.
Further, since blood flow can take away heat, heating ablation effect is influenced seriously by hemoperfusion, and heating ablation is to cause tissue bad
Extremely, it is not easy to excrete.
Nano-knife ablation is that high voltage electric field is transmitted to tumour cell in the form of micropulse, changes cell transmembrane electricity
Gesture causes nanoscale hole on lipid bilayer cell membrane, increases permeability of cell membrane, causes target tumor cell irreversible
Electroporation eventually leads to tumor mortality.Nano-knife ablation is living cells, the cellular matrix and cell week that it theoretically retains
The structure enclosed, it is smaller to the tissue effects such as blood vessel, bile duct and nerve in treatment region, it is typically only restorability damage, in structure
It functionally still maintains complete.And due to nano-knife ablation be non-thermal ablation, do not influenced by blood flow, can be with blood vessel around
Generate complete cell death.
Nano-knife ablation procedure will lead to membrane structure and intracellular if the electric field applied is more than a certain threshold value
Stable state permanent damage causes to be immunoreacted, so as to cause natural death of cerebral cells.According to different tumours, impulse electric field strength is
1.5kV/cm, pulse width are 20~1000 μ s, and impulse amplitude determines that the volume of single ablation, the volume of amplitude single ablation are got over
Greatly, maximum output current is up to 50A, impulse amplitude 3kV, and pulse power is up to 150kW；Impulse amplitude is 4.5kV, pulse
Power is up to 225kW.In therapeutic process, when irreversibility electroporation occurs for tissue, biological tissue's impedance was with treating
Journey changes constantly, needs to calculate human body impedance, guarantees electroporation effect, real-time monitoring ablation effect.It is clinically more at present
Using unipolarity square-wave pulse, impulse electric field strength used is 1.5kV/cm, and pulse width is 100 μ s, and impulse amplitude is 3kV's
Square wave.Due to biological tissue's impedance as therapeutic process changes constantly, the impedance between therapeutic electrode can drop to 60 Ω, directly
It is lower to connect measurement human body impedance safety, that is to say, that in case of short trouble, energy will all be released to patient, will make
At catastrophic effect
Summary of the invention
The technical problem to be solved by the present invention is in view of the deficienciess of the prior art, providing a kind of isolated human body impedance
Detection circuit and detection method, detection circuit according to the present invention can be carried out human body impedance detection, guarantee electroporation effect, play
Real-time monitoring ablation effect, and isolated AD conversion technology is used, improve the electrical peace of irreversibility electroporation device circuit
Quan Xing reduces the volume and weight of electroporation device circuit.To achieve the goals above, the technical solution adopted by the present invention is such as
Under:
According to an aspect of the present invention, a kind of isolated human body impedance detection circuit, including impulse generator, number are provided
According to more than processing unit, data display unit, one or more therapeutic electrodes and a channel or two channels and for adopting
Collect human body impedance impedance collection unit, the output end of the impulse generator respectively with one or more of therapeutic electrodes
Input terminal connection, the acquisition input terminal of the impedance collection unit are connect with the output end of the therapeutic electrode respectively, the resistance
The acquisition output end of anti-acquisition unit is connect with the input terminal of the data processing unit, the output end of the data processing unit with
The data display unit connection, using therapeutic electrode as test point, the acquisition input terminal acquisition testing point of impedance collection unit
Treatment pulse signal voltage and current.
Preferably, each impedance collection unit includes at least a voltage acquisition unit and a current acquisition list
Member, the therapeutic electrode include voltage detecting electrode and current detection electrode, the output end of the impulse generator respectively with institute
The input terminal for stating voltage detecting electrode is connected with the input terminal of current detection electrode, the voltage acquisition unit includes voltage low pass
Filter circuit, voltage peak holding circuit, voltage signal attenuator circuit and the isolated A/D converter of voltage, the voltage detecting
The output end of electrode is connect by voltage low-pass filter circuit with the input terminal of the voltage peak holding circuit, the voltage peak
The output end of holding circuit passes through voltage signal attenuator circuit, the isolated A/D converter of voltage and the data processing unit
The connection of first detection input；The current acquisition unit includes electric current low-pass filter circuit, current peak holding circuit, electric current
Signal attenuation circuit passes through electric current low-pass filtering electricity with formula A/D converter, the output end of the current detection electrode is galvanically isolated
Road is connect with the input terminal of the current peak holding circuit, and the output end of the current peak holding circuit is declined by current signal
Powered down road is galvanically isolated formula A/D converter and connect with the second detection input of the data processing unit.
Preferably, the voltage low-pass filter circuit includes resistance R1 and capacitor C1, the voltage peak holding circuit packet
The first operational amplifier U1, rectifier diode S1 and capacitor C2 are included, the voltage signal attenuator circuit includes resistance R2, resistance R3
With second operational amplifier U2, one end of the resistance R1 is connect with the output end of the voltage detecting electrode, the resistance R1
The other end connect respectively with the electrode input end of one end of capacitor C1 and the first operational amplifier U1, first operational amplifier
The output end of U1 is connect with the anode of the rectifier diode S1, the cathode of rectifier diode S1 respectively with capacitor C2 one
End, resistance R2 one end connected with the negative input of the first operational amplifier U1, the other end of the resistance R2 respectively with institute
The one end for stating resistance R3 is connected with the electrode input end of second operational amplifier U2, the output end point of second operational amplifier U2
It is not connect with the acquisition input terminal of the negative input of second operational amplifier U2 and the isolated A/D converter of voltage, the voltage
The acquisition output end of isolated A/D converter is connect with the first detection input of the data processing unit U, the capacitor C1
The other end, the other end of capacitor C2 and the other end of resistance R3 be connected to ground respectively.
Preferably, the isolated A/D converter uses AMC1303MX converter chip.
Preferably, the voltage pulse amplitude of the detecting electrode output is 5V-3000V.
Preferably, multiple detecting electrodes are in the shape of a spiral or circular row is fixed on one substrate, each detection
Electrode is 8mm-12mm, and each electrode forms spiral distribution on human body or circular ring shape is looped around on human body, and detection human body is deep
When the impedance of portion's tissue, additional detections electrode is not used alone, but is detected by therapeutic electrode, avoids to the two of human body
Secondary damage reduces the volume and weight of detection device circuit, and each electrode forms a test point with human contact respectively, from
And the resolving accuracy of impedance detection is improved, to detect that each resistance corresponds to the impedance summation between human testing point.
The present invention also provides a kind of detection methods of isolated human body impedance, and described detection method includes the following steps:
Step S1, using therapeutic electrode as test point, at least through a voltage acquisition unit and a current acquisition list
The voltage triggering signal and current trigger signal of first acquisition testing point, the therapeutic electrode include voltage detecting electrode and electric current inspection
Electrode is surveyed, by the test point of voltage detecting electrode and current detection electrode contact human body, then to voltage detecting electrode and electric current
Detecting electrode applies trigger signal source, and voltage detecting electrode and current detection electrode is made to obtain voltage triggering signal and electric current respectively
Trigger signal；
Step S2 protects the voltage triggering signal and current trigger signal through signal decaying, low pass filtered, voltage crest value respectively
It holds, be sent into isolated A/D converter after signal is decayed again, obtain voltage digital signal and current digital signal；
The voltage digital signal and current digital signal are sent into data processing unit by step S3, and by with it is pre-
The resistance first set=voltage/current computation model is calculated, and surveyed human body impedance value is calculated, and show list in data
It is shown in member.
Above scheme is it is further preferred that the detection method further includes by one or more voltage detecting electrodes and electricity
The test point for flowing the detection point contact human body of detecting electrode contact human body, to one or more voltage detecting electrodes and current detecting
Electrode applies preset trigger signal source, is continuously measured within the predetermined time, get multiple voltage triggering signals and
The sampling point value of current trigger signal, then by multiple sampling point values and preset resistance=voltage/current computation model into
Row is calculated and is averaged, and surveyed human body impedance value is calculated.
In conclusion the present invention is due to using above technical scheme, beneficial effect:
(1), the detection circuit invented can be carried out human body impedance detection, guarantees electroporation effect, can play real-time monitoring and disappear
Melt effect, and use isolated AD conversion technology, improves the electrical security of irreversibility electroporation device circuit, reduce
The volume and weight of perforating apparatus circuit.
(2), peak holding circuit of the invention can remain to the peak value of pulse of the 100 μ s in each channel or so pulsewidth
300 μ s pulsewidths, peak holding circuit by the peak holding after pulse-shaping for a period of time, as the defeated of follow-up signal attenuator circuit
Enter.High-frequency signal carries out AD data conversion after signal attenuation module carries out signal decaying, it is most important that carries out to human body
The isolation of impedance detection, the present invention are detected using isolated human body impedance, not only observe electroporation effect, and have isolation
Effect.
Detailed description of the invention
In order to illustrate more clearly of present example or technical solution in the prior art, to embodiment or will show below
There is in technical description required attached drawing do simply to introduce, it is clear that the accompanying drawings in the following description is only of the invention some
Example under the premise of not paying creativeness, can also be obtained according to these attached drawings to those skilled in the art
Other attached drawings.
Fig. 1 is a kind of functional block diagram of isolated human body impedance detection circuit of the invention；
Fig. 2 is a kind of circuit diagram of isolated human body impedance detection circuit of the invention；
Fig. 3 is a kind of overhaul flow chart of the detection method of isolated human body impedance of the invention.
Specific embodiment
Below in conjunction with the attached drawing in present example, technical solution in the embodiment of the present invention carries out clear, complete
Ground description, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Based on this
Embodiment in invention, every other reality obtained by those of ordinary skill in the art without making creative efforts
Example is applied, shall fall within the protection scope of the present invention.
According to an aspect of the present invention, in conjunction with Fig. 1, the present invention provides a kind of isolated human body impedance detection circuit,
Including impulse generator, data processing unit, data display unit, one or more therapeutic electrodes and a channel or two
More than channel and for acquiring the impedance collection unit of human body impedance, the output end of the impulse generator respectively with one or more
The input terminal of a therapeutic electrode connects, and the acquisition input terminal of the impedance collection unit is defeated with the therapeutic electrode respectively
Outlet connection, the acquisition output end of the impedance collection unit is connect with the input terminal of the data processing unit, at the data
The output end of reason unit is connect with the data display unit, using therapeutic electrode as test point, the acquisition of impedance collection unit
The voltage and current of the treatment pulse signal of input terminal acquisition testing point.In the present invention, each impedance collection unit is extremely
It less include a voltage acquisition unit and a current acquisition unit, the therapeutic electrode includes voltage detecting electrode and electric current inspection
Electrode is surveyed, the output end of the impulse generator is defeated with the input terminal of the voltage detecting electrode and current detection electrode respectively
Enter end connection, the voltage acquisition unit includes voltage low-pass filter circuit, voltage peak holding circuit, voltage signal decaying electricity
The output end of road and the isolated A/D converter of voltage, the voltage detecting electrode passes through voltage low-pass filter circuit and the electricity
The input terminal connection of peak holding circuit is pressed, the output end of the voltage peak holding circuit passes through voltage signal attenuator circuit, electricity
Isolated A/D converter is pressed to connect with the first detection input of the data processing unit；The current acquisition unit includes
Electric current low-pass filter circuit, current peak holding circuit, current signal attenuator circuit and it is galvanically isolated formula A/D converter, it is described
The output end of current detection electrode is connect by electric current low-pass filter circuit with the input terminal of the current peak holding circuit, should
The output end of current peak holding circuit passes through current signal attenuator circuit, is galvanically isolated at formula A/D converter and the data
Manage the second detection input connection of unit.
In the present invention, referring to figs. 1 and 2, the voltage low-pass filter circuit includes resistance R1 and capacitor C1, institute
Stating voltage peak holding circuit includes the first operational amplifier U1, rectifier diode S1 and capacitor C2, the voltage signal decaying
Circuit includes resistance R2, resistance R3 and second operational amplifier U2, one end of the resistance R1 and the voltage detecting electrode
Output end connection, the other end of the resistance R1 electrode input end with one end of capacitor C1 and the first operational amplifier U1 respectively
Connection, the output end of first operational amplifier U1 are connect with the anode of the rectifier diode S1, rectifier diode S1's
Cathode is connect with the negative input of one end of capacitor C2, one end of resistance R2 and the first operational amplifier U1 respectively, the electricity
The other end of resistance R2 is connect with the electrode input end of one end of the resistance R3 and second operational amplifier U2 respectively, second fortune
The output end of amplifier U2 is calculated to adopt with the negative input of second operational amplifier U2 and the isolated A/D converter of voltage respectively
Collect input terminal connection, the first detection of the acquisition output end and the data processing unit U4 of the isolated A/D converter of the voltage
Input terminal connection, the other end of the other end of the capacitor C1, the other end of capacitor C2 and resistance R3 are connected to ground respectively.At this
In invention, as shown in Fig. 2, electric current low-pass filter circuit, current peak holding circuit, current signal in the current acquisition unit
Attenuator circuit and the circuit structure principle for being galvanically isolated formula A/D converter and the voltage low-pass filtering electricity in voltage acquisition unit
Road, voltage peak holding circuit, the structural principle of voltage signal attenuator circuit are identical, and the electric current low-pass filter circuit includes electricity
R11 and capacitor C11 is hindered, the current peak holding circuit includes second operational amplifier U11, rectifier diode S11 and capacitor
C12, the current signal attenuator circuit include resistance R12, resistance R13 and third operational amplifier U12, the resistance R11's
One end is connect with the output end of the current detection electrode, the other end of the resistance R11 respectively with one end of capacitor C11 and
The electrode input end of two operational amplifier U11 connects, the output end and the rectifier diode of second operational amplifier U11
The anode of S11 connects, and the cathode of rectifier diode S11 is transported with one end of capacitor C12, one end of resistance R12 and second respectively
Calculate amplifier U11 negative input connection, the other end of the resistance R12 respectively with one end of the resistance R13 and third
The electrode input end of operational amplifier U12 connects, the output end of third operational amplifier U12 respectively with third operational amplifier
The negative input of U12 is connected with the acquisition input terminal for being galvanically isolated formula A/D converter U13, this is galvanically isolated formula A/D converter
The acquisition output end of U13 is connect with the second detection input of the data processing unit U4, the other end of the capacitor C11,
The other end of capacitor C12 and the other end of resistance R13 are connected to ground respectively.
It is further elaborated below with the working principle of voltage acquisition unit, the voltage arteries and veins of the voltage detecting electrode output
Rushing amplitude is 5V-3000V, and the voltage peak holding circuit rectifies diode S1 and capacitor C2 by the first operational amplifier U1
It constitutes, the impulse wave that low-pass filter exports detecting electrode is constituted by resistance R1 and capacitor C1 and is filtered, noise is eliminated
Interference；Voltage attenuator is constituted by resistance R2 and resistance R3, input voltage is decayed within the scope of 250mv, to prevent voltage isolation
Formula A/D converter U3 outranges, and isolated A/D converter U3 is undertaken by AMC1303MX chip, which realizes two kinds of functions,
One is to the buffer action of human body impedance detection, the second is carrying out AD data conversion.The output of voltage attenuator is through the second fortune
After calculating the operation follower that amplifier is constituted, it is re-fed into the port AINN of the isolated A/D converter U3 of voltage, and by capacitor C3 electricity
It is in parallel to hold C4, nominal voltage provided by the port analog power 5V AVDD is filtered；Isolated A/D converter U3's is defeated
Outlet is the digital end of isolation, and the digital signal of modulation is exported by the port DOUT, and in parallel by capacitor C5 and capacitor C6, to number
Power supply provided by power supply 5V DVDD digital port is filtered, and in the present invention, the output end DOUT of AMC1303MX is exported
Data input signal of the digital signal as data processing unit, using 32 floating-point microsever U3 as core processing
Device is handled and is analyzed to signal collected, and the result of processing is shown in data display unit, microprocessor
U3 is calculated using TMS320F2837X processor chips, and isolated human body impedance detection circuit uses AMC1303MX chip,
The chip is high-precision modulator isolation input and output.If short trouble occurs for switch, output end is isolated with input terminal, energy
It blocks energy to discharge to patient, to protect the safety of patient, not only can be carried out AD data conversion using AMC1303MX chip,
The buffer action detected to human body impedance is most importantly carried out, guarantees the safety to human body.AMC1303MX is a high-precision
Delta-sigma (Δ Σ) modulator, output are isolated with input circuit by the double isolated gates of the higher condenser type of magnetic field noise immunity.
(device is directed to according to DINV DE V 0884-10 (semiconductor devices-safe insulation magnetic capacitance coupler standard), UL1577
Part insulation pressure resistance, voltage breakdown grading standard) and CSA standard (Canadian Standards Association), it should
The authenticated enhanced isolation that can provide up to 7000V peak value of isolated gate.When being used cooperatively with isolated power supply, the isolated tune
Device processed can prevent the noise current of common mode high voltage transmission line road from entering local system ground, thus interference or damage low-voltage electricity
Road prevents lower voltage devices from damaging.Meanwhile isolated modulator AMC1303MX for be directly connected to shunt resistance device or its
Its low-voltage-grade signal source is optimized, at the same have outstanding direct current with exchange performance.By using number appropriate
Filter extracts bit stream, can realize 16 bit resolutions of 85dB (13.8ENOB) dynamic range under 78kSPS data rate.
In high side, modulator is powered by 5V (AVDD) nominal voltage, and it is then nominal by 3.3V or 5V power supply (DVDD) that digital interface is isolated
Power voltage supply.After impedance detection is activated, the signal of detecting electrode detection is square-wave pulse, in pulse width determining electrode
Output pulse width.The peak value of pulse of each 100 μ s of channel or so pulsewidth is remained to 300 μ s pulsewidths by peak holding circuit,
Peak holding circuit by the peak holding after pulse-shaping for a period of time, the input as follow-up signal attenuator circuit.High frequency letter
Number by signal attenuation module carry out signal decaying after, as the input of isolated A/D converter, AMC1303MX chip is not only
It can be carried out AD data conversion, it is most important that carry out the isolation detected to human body impedance, according to resistance=voltage/current, calculate
Obtain human body impedance.The present invention is detected using isolated human body impedance, not only observable electroporation effect, and has isolation
Effect.
In the present invention, in conjunction with Fig. 1, Fig. 2 and Fig. 3, a kind of detection method of isolated human body impedance is additionally provided, including
Following steps:
Step S1, using therapeutic electrode as test point, at least through a voltage acquisition unit and a current acquisition list
The voltage triggering signal and current trigger signal (voltage and current for the treatment of pulse signal) of first acquisition testing point, the treatment electricity
Pole includes voltage detecting electrode and current detection electrode, by the detection of voltage detecting electrode and current detection electrode contact human body
Then point applies trigger signal source to voltage detecting electrode and current detection electrode, make voltage detecting electrode and current detecting electricity
Pole obtains voltage triggering signal and current trigger signal respectively；One or more voltage detecting electrodes can be passed through in the present invention
With the test point of current detection electrode contact human body, applies trigger signal source to voltage detecting electrode and current detection electrode, make
Voltage detecting electrode and current detection electrode obtain voltage triggering signal and current trigger signal respectively；Multiple voltage detectings
Electrode and current detection electrode are in the shape of a spiral or circular row is fixed on one substrate, and each detecting electrode is 8mm-
12mm, each electrode carry out multiplicating measurement as a test point, by multiple test points, every time acquisition 128 or 256
The trigger signal of sampled point；To same test point test constantly 10s, so that the human body impedance value measured is more accurate；
Step S2 protects the voltage triggering signal and current trigger signal through signal decaying, low pass filtered, peak value respectively
It holds, be sent into isolated A/D converter after signal is decayed again, obtain voltage digital signal and current digital signal, as shown in Figure 3；
The trigger signal source of application be nano-knife impulse generator, nano-knife impulse generator generate electric pulse, impulse waveform, amplitude,
Repetition rate, pulse width and pulse number directly affect therapeutic effect, are needed thus by voltage triggering signal and current trigger
Signal isolation and A/D conversion could be sent into after signal decayed, peak holding and signal are decayed again to obtain voltage number
According to output and current data output；Due to if the electric field applied is more than a certain threshold value, will lead in nano-knife ablation procedure
Membrane structure and cell homeostasis permanent damage, cause to be immunoreacted, so as to cause natural death of cerebral cells.Therefore, pass through electric arteries and veins
The technology of punching makes to generate irreversible electroporation (IRE) on the phosphatide or phospholipoprotein film of mammal, by mammalian cell
Representative value is up to 2.5kV/cm, for this purpose, the impulse electric field strength of nano-knife impulse generator output must be sufficiently high, just can guarantee
The effect of electroporation, when mammalian cell carries out irreversible electroporation, nano-knife impulse generator uses square wave output wave
Shape, being applied to voltage range constant in detecting electrode (therapeutic electrode 1 and therapeutic electrode 2) is 500V-5000V, repetition rate
Between 1-2Hz, pulse width generallys use 1 μ s to hundreds of μ s, and pulse number is between 1~199 time, for this purpose, passing through detection
When electrode carries out nano-knife ablation to human body, and detect human body impedance, obtains the voltage data and electric current number when ablation
According to real-time monitoring ablation effect can be played to guarantee electroporation effect；
The voltage digital signal and current digital signal are sent into data processing unit by step S3, and by with it is pre-
The resistance first set=voltage/current computation model is calculated, and surveyed human body impedance value is calculated, and show list in data
It is shown in member.
In the present invention, it is connect by the test point that one or more voltage detecting electrodes and current detection electrode contact human body
The test point for touching human body applies preset trigger signal source, In to one or more voltage detecting electrodes and current detection electrode
It is continuously measured in the scheduled time, gets the sampling point value of multiple voltage triggering signals and current trigger signal, then will
Multiple sampling point values calculate with preset resistance=voltage/current computation model and be averaged, and are calculated and are surveyed
Human body impedance value, multiple voltage detecting electrodes and current detection electrode are in the shape of a spiral or circular row is fixed on a base
On plate, each detecting electrode is 8mm-12mm, to same test point test constantly 10s, the multiple repairing weld in this section of time of measuring,
128 or 256 sampled points are obtained, then calculate by resistance=voltage/current computation model and average, so that measuring
Human body impedance value it is more accurate.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all in the use of the new type
Spirit and principle within, any modification, equivalent replacement, improvement and so on should be included within the protection scope of invention.
Claims (8)
1. a kind of isolated human body impedance detection circuit, it is characterised in that: including impulse generator, data processing unit, data
More than display unit, one or more therapeutic electrode and a channel or two channels and the impedance for acquiring human body impedance
Acquisition unit, the output end of the impulse generator is connect with the input terminal of one or more therapeutic electrodes respectively, described
The acquisition input terminal of impedance collection unit is connect with the output end of the therapeutic electrode respectively, the acquisition of the impedance collection unit
Output end is connect with the input terminal of the data processing unit, the output end of the data processing unit and the data display unit
Connection.
2. a kind of isolated human body impedance detection circuit according to claim 1, it is characterised in that: each impedance is adopted
Collect unit and include at least a voltage acquisition unit and a current acquisition unit, the therapeutic electrode includes voltage detecting electrode
And current detection electrode, the output end of the impulse generator input terminal and current detecting with the voltage detecting electrode respectively
The input terminal connection of electrode, the voltage acquisition unit include voltage low-pass filter circuit, voltage peak holding circuit, voltage letter
The output end of number attenuator circuit and the isolated A/D converter of voltage, the voltage detecting electrode passes through voltage low-pass filter circuit
It is connect with the input terminal of the voltage peak holding circuit, the output end of the voltage peak holding circuit is decayed by voltage signal
The isolated A/D converter of circuit, voltage is connect with the first detection input of the data processing unit；The current acquisition list
Member includes electric current low-pass filter circuit, current peak holding circuit, current signal attenuator circuit and is galvanically isolated formula A/D conversion
Device, the output end of the current detection electrode pass through the input terminal of electric current low-pass filter circuit and the current peak holding circuit
Connection, the output end of the current peak holding circuit by current signal attenuator circuit, be galvanically isolated formula A/D converter with it is described
Second detection input of data processing unit connects.
3. a kind of isolated human body impedance detection circuit according to claim 2, it is characterised in that: the voltage low pass filtered
Wave circuit includes resistance R1 and capacitor C1, and the voltage peak holding circuit includes the first operational amplifier U1, rectifier diode
S1 and capacitor C2, the voltage signal attenuator circuit include resistance R2, resistance R3 and second operational amplifier U2, the resistance R1
One end connect with the output end of the voltage detecting electrode, the other end of the resistance R1 respectively with one end of capacitor C1 and
The electrode input end of one operational amplifier U1 connects, and the output end of first operational amplifier U1 is with the rectifier diode S1's
Anode connection, the cathode of rectifier diode S1 respectively with one end of capacitor C2, one end of resistance R2 and the first operational amplifier
The negative input of U1 connects, the other end of the resistance R2 respectively with one end of the resistance R3 and second operational amplifier U2
Electrode input end connection, the output end of the second operational amplifier U2 negative input with second operational amplifier U2 respectively
Connected with the acquisition input terminal of the isolated A/D converter of voltage, the acquisition output end of the isolated A/D converter of the voltage with it is described
The first detection input of data processing unit U connects, the other end of the capacitor C1, the other end of capacitor C2 and resistance R3
The other end is connected to ground respectively.
4. a kind of isolated human body impedance detection circuit according to claim 2 or 3, it is characterised in that: it is described isolated
A/D converter uses AMC1303MX converter chip.
5. a kind of isolated human body impedance detection circuit according to claim 1 or 2 or 3, it is characterised in that: the detection
The voltage pulse amplitude of electrode output is 5V-3000V.
6. a kind of isolated human body impedance detection circuit according to claim 1, it is characterised in that: multiple detection electricity
Pole is in the shape of a spiral or circular row is fixed on one substrate, and each detecting electrode is 8mm-12mm.
7. a kind of inspection for wanting a kind of isolated human body impedance detection circuit to carry out human body impedance using 2 or 3 any rights
The method of survey, which comprises the following steps:
Step S1 is adopted using therapeutic electrode as test point at least through a voltage acquisition unit and a current acquisition unit
Collect the voltage triggering signal and current trigger signal of test point, the therapeutic electrode includes voltage detecting electrode and current detecting electricity
Pole, by the test point of voltage detecting electrode and current detection electrode contact human body, then to voltage detecting electrode and current detecting
Electrode applies trigger signal source, and voltage detecting electrode and current detection electrode is made to obtain voltage triggering signal and current trigger respectively
Signal；
Step S2, by the voltage triggering signal and current trigger signal respectively through signal decaying, low pass filtered, pressure peak holding,
Signal is sent into isolated A/D converter after decaying again, obtain voltage digital signal and current digital signal；
The voltage digital signal and current digital signal are sent into data processing unit by step S3, and by with set in advance
Fixed resistance=voltage/current computation model is calculated, and surveyed human body impedance value is calculated, and in data display unit
It is shown.
8. a kind of detection method of isolated human body impedance according to claim 6, which is characterized in that further include by one
The test point of the detection point contact human body of a or multiple voltage detecting electrodes and current detection electrode contact human body, to one or more
A voltage detecting electrode and current detection electrode apply preset trigger signal source, are continuously measured within the predetermined time,
Get the sampling point value of multiple voltage triggering signals and current trigger signal, then by multiple sampling point values and preset electricity
Resistance=voltage/current computation model calculate and is averaged, and surveyed human body impedance value is calculated.
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| CN201910820243.2A CN110448374B (en) | 2019-09-01 | Isolated human body impedance detection circuit and detection method |
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| CN201910820243.2A CN110448374B (en) | 2019-09-01 | Isolated human body impedance detection circuit and detection method |
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| CN112039178A (en) * | 2020-08-31 | 2020-12-04 | 赛诺微医疗科技（浙江）有限公司 | Control method and device of irreversible electroporation ablation device |
| CN113143242A (en) * | 2021-04-12 | 2021-07-23 | 浙江大学 | Circuit system for measuring human body spinal bone reactance |
| CN114081500A (en) * | 2020-07-30 | 2022-02-25 | 京东方科技集团股份有限公司 | Signal acquisition circuit and physiological detection equipment |
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