CN206992984U

CN206992984U - A kind of irreversible electroporation apparatus of high frequency

Info

Publication number: CN206992984U
Application number: CN201720297729.9U
Authority: CN
Inventors: 单纯玉; 郝丽俊
Original assignee: Shanghai University of Medicine and Health Sciences
Current assignee: Shanghai Bekada Medical Technology Co.,Ltd.
Priority date: 2017-03-24
Filing date: 2017-03-24
Publication date: 2018-02-09
Anticipated expiration: 2027-03-24

Abstract

The utility model belongs to technical field of medical instruments,More particularly to a kind of irreversible electroporation apparatus of high frequency,Including power converting circuit,Accumulator,Isolation type high frequency converts output circuit,Signal controller,Electrode and power supply,The output end of the power supply is connected with power converting circuit,The power converting circuit passes sequentially through accumulator,Isolation type high frequency conversion output circuit is connected with electrode,The signal controller respectively with power converting circuit,The control signal connection of isolation type high frequency conversion output circuit,Electrode is set to be discharged so as to control isolation type high frequency to convert the pulse output signals of output circuit by signal controller,The utility model makes living cells produce irreversible electrical breakdown,Increase the uniformity of irreversibility point perforation,Reducing electroporation stimulates contraction of muscle caused by nerve,Available for treatment of solid tumor in body or vitro treatment experimental study.

Description

A kind of irreversible electroporation apparatus of high frequency

Technical field

The utility model belongs to technical field of medical instruments, and in particular to a kind of irreversible electroporation apparatus of high frequency.

Background technology

Irreversibility electroporation refers to cause cell membrane that permanent penetrating mistake occurs by applying high intensity external electrical field Journey, membrane potential caused by external electrical field cause the interior formation of cell membrane numerous nanometer level microporous, destroy cell homeostasis, such as The electric field that fruit is applied is less than a certain threshold value, then this event is temporary, and after external electrical field is removed, cell membrane recovers just Often (invertibity electroporation), this so-called " reversible electroporation " have become an important technology in molecular medicine, are used for Medicine is helped by cell membrane, if however, the electric field applied exceedes a certain threshold value, membrane structure and into the cell will be caused Stable state permanent damage, so as to cause cell death, latter effect is used as a kind of new minimally invasive ablation techniques recently. As economic and society development, the life of healthy serious threat people, tumour more turn into the main prestige of global human health The side of body, 2013《World's cancer report》It has been shown that, global tumor invasion number and death toll are in ascendant trend year by year, tumor invasion 12,700,000 rise to 2012 14,100,000 from 2008 are counted, death toll also rose to from 7,600,000 people of 2008 8,200,000 people of 2012.Irreversibility electroporation treatment tumour technology, as a kind of new tumor therapeuticing method, have existing The incomparable advantage of physical therapy modalities, shows good potential applicability in clinical practice.However, irreversibility electroporation ablation Also some inferior positions are implicitly present in.Melting lung or during lobus sinister liver neoplasm, due to these positions all near heart (<1.7 Cm), high field can cause the ventricular fibrillation in arrhythmia cordis, including preclinical study, ecg-gating type irreversibility electricity Tunnel ablation applies electric field using cardiac cycle synchronization, can overcome this inferior position.In addition, irreversibility electroporation ablation institute The situation of muscle strong contraction occurs in high voltage, typically slows down muscle by injection muscles relaxant in treatment at present Shrink, when the repetition rate of used pulse is 1Hz, often inject 1 subpulse, muscle just shrinks 1 time, clinically at present to adopt more With unipolarity square-wave pulse.Traditionally impulse electric field strength used is 1.5kV/cm, and pulse width is 100 μ s, and is obtained bright Aobvious therapeutic effect.Clinic uses square wave of the impulse amplitude for 3kV with electroporation apparatus, and biological tissue's impedance exists with therapeutic process Constantly change, the impedance between therapeutic electrode can drop to 60 Ω, and therefore, maximum output current will reach up to 50A, pulse power To 150kW.

Irreversibility electroporation ablation techniques are a kind of non-heated property ablation techniques, can not compared with other ablation methods Inverse property electroporation has some theoretic advantages, and first, ablation time is very short, and it is about 3cm that diameter can be created in 1min Ablation areas.Secondly as irreversibility electroporation is non-thermal ablation, there is no " heat sink " effect, can be produced around blood vessel Raw complete cell death.Again, irreversibility electroporation ablation be living cells, the cellular matrix that it retains in theory and The structure of cell peripheral, so big blood vessel and bile duct are structurally and functionally remaining in that completely.Again, irreversibility electricity is utilized When tunnel ablation liver edge or top, to the possibility very little of the indirect injury of neighbouring structure.3rd, cause cell death Mechanism is apoptosis, rather than necrosis.The advantages of Apoptosis is that to remove apoptotic cell, phagocyte will simultaneously by Immune intervention Apoptotic cell is removed as the death process of normal cell so as to promote the regeneration of normal structure and reparation.Therefore through not Area for treatment can be substituted so as to recover original function by normal cell in a short time after invertibity electroporation treatment, finally, Real-time ultrasound can be used for Treatment monitoring, and can depict area for treatment exactly, and the region and Immunohistopathology On the cell death region correlation seen it is very good.These features exactly quasi-complement deficiency of thermal ablation techniques.And high frequency electric pair The excitable tissues such as nerve, muscle without stimulating, medical field is widely used in, such as electric knife, RF ablation；Up to a watt Loews (Davalos R V) has then carried out irreversibility electroporation experiment using ambipolar high-frequency pulse string to the brain tissue of mouse, real Contraction of muscle phenomenon is not found during testing, but required pulse voltage amplitude is higher, Christoffer Etc. (Christopher) brain tumor of experimental mouse is melted using 250kHz or 500kHz diphasic pulse string, do not sent out Now obvious contraction of muscle, the delicate level pulse of output is modulated by high-frequency signal and produces bipolarity positive pulse, negative arteries and veins Punching, this bipolarity high frequency burst waveforms can not only form symmetry electroporation on cell membrane, but also can be obvious The stimulation to nerve is reduced, while the contraction of muscle will not be caused.

Utility model content

For the purpose of this utility model to solve the above mentioned problem and deficiency of prior art, the utility model provides a kind of height Frequently irreversible electroporation apparatus, living cells is produced irreversible electrical breakdown using high frequency bi-phase modulated square wave, increase irreversibility The uniformity of point perforation, contraction of muscle caused by nerve, to achieve these goals, this practicality are stimulated to reduce electroporation The technical scheme of new use is as follows:

A kind of irreversible electroporation apparatus of high frequency, including power converting circuit, accumulator, isolation type high frequency conversion output electricity Road, signal controller, electrode and power supply, the output end of the power supply are connected with power converting circuit, the power converting circuit according to It is secondary by accumulator, isolation type high frequency convert output circuit be connected with electrode, the signal controller respectively with power conversion The control signal connection of circuit, isolation type high frequency conversion output circuit, by signal controller so as to controlling isolation type high frequency The pulse output signals of conversion output circuit make electrode be discharged.

Preferably, the power converting circuit is all the way, or more than two-way to be connected in parallel, the isolation type high frequency conversion Output circuit is all the way, or more than two-way to be connected in parallel, by the power converting circuit all the way or more than two-way with all the way or Accumulator more than two-way is attached, the accumulator and the isolation type high frequency conversion output electricity all the way or more than two-way Road is attached.

Preferably, the power converting circuit includes pulse-width modulation circuit, booster circuit, filter circuit and rectification circuit, Power supply is connected by the signal output part of the pulse-width modulation circuit and the control terminal of booster circuit, the output end of the booster circuit Pass sequentially through the filter circuit, rectification circuit and accumulator connect, the power supply also with the booster circuit, filter circuit Input connection.

Preferably, the pulse-width modulation circuit include PWM controller, resistance R1, resistance R2, resistance R3, adjustable resistance R4, Resistance R5, electric capacity C1 and electric capacity C2, the booster circuit include field-effect Q1, field-effect Q2 and step-up transformer T1, the storage Energy circuit includes electric capacity C3 and electric capacity C4, and the rectification circuit includes diode D1 and diode D2, one end of the resistance R1 It is connected with the oscillating discharge output end of PWM controller, one end of the resistance R2 and the vibration timing resistor of PWM controller input End connection, electric capacity C1 one end and the vibration timing capacitor input of PWM controller connect, the other end, the electricity of the resistance R1 The other end and the electric capacity C1 other end for hindering R2 are all connected to ground, one end of the resistance R3, adjustable resistance R4 one end, can Resistance R4 centre cap is adjusted all to be connected with the anti-phase error input of PWM controller, the adjustable resistance R4 other end is connected to ground, First complementary output end of the PWM controller is connected with FET Q1 grid, the second complementary output end of PWM controller It is connected with FET Q2 grid, the drain electrode of the FET Q1 is connected with one end of step-up transformer T1 primary side taps, FET Q1 source electrode outside cut-off signals input, resistance R5 one end, the FET Q2 with PWM controller respectively Source electrode connection, the other end of the resistance R5 is connected to ground, drain electrode and the step-up transformer T1 primary sides of the FET Q2 The other end connection of tap, the input with the power supply, filter circuit are connected step-up transformer T1 centre cap respectively, institute Stating one end of step-up transformer T1 secondary taps, the anode with diode D1, diode D2 negative electrode are connected respectively, the boosting The negative pole with electric capacity C3, electric capacity C4 positive pole are connected the other end of transformer T1 secondary taps respectively, the positive pole of the electric capacity C3 The other end with resistance R3, diode D1 negative electrode, the input of isolation type high frequency conversion output circuit are connected respectively, the electricity The negative pole for holding C4 is connected to ground, and the electric capacity input of the PWM controller and the input/output control port of signal controller connect Connect.

Preferably, the power supply is 0~36V DC voltage, and frequency is 30~100kHz.

Preferably, the square-wave pulse signal that the frequency of the PWM controller output is 50kHz, what the PWM controller used Model SG3525 chips.

Preferably, the electric capacity C3, electric capacity C4 capacitance are not less than 2200 μ F, and pressure voltage is not less than 450V.

Preferably, the isolation type high frequency conversion output circuit includes high speed pulse width modulation controller, the first numeral isolation Driver, the second digital driver for isolating, the first power amplifier, the second power amplifier, the 3rd power amplifier, the 4th work( Rate amplifier and isolating transformer T2, the Enable Pin of the first digital driver for isolating, the second digital driver for isolating make Energy end is connected with the input/output control port of signal controller respectively, and the first of the high speed pulse width modulation controller is complementary Output end is connected with the first input end of the first digital driver for isolating, the second input end of the second digital driver for isolating, Second input of the second complementary output end of the high speed pulse width modulation controller and the first digital driver for isolating, the second number The first input end connection of word driver for isolating；

The drain electrode of first power amplifier, the drain electrode of the 3rd power amplifier and the output end of the accumulator connect Connect, the first power amplifier grid is connected with the first drive output of the first digital driver for isolating, the first power amplifier Source electrode be connected to ground, the grid of the second power amplifier is connected with the second drive output of the first digital driver for isolating, The source electrode of second power amplifier is connected to ground, the first drive of the grid of the 3rd power amplifier and the second digital driver for isolating Dynamic output end connection, the grid of the 4th power amplifier and the second drive output of the second digital driver for isolating connect Connect, the source electrode of the 4th power amplifier is connected to ground, the source electrode of first power amplifier, the second power amplifier Drain electrode is connected with a tap of isolating transformer T2 primary sides, the source electrode of the 3rd power amplifier, the 4th power amplifier Drain electrode is connected with another tap of isolating transformer T2 primary sides, a tap of the isolating transformer T2 secondary and the positive pole of electrode Connection, another tap of the isolating transformer T2 secondary and the negative pole of electrode connect.

Preferably, the described first digital driver for isolating, the second digital driver for isolating use model Si82390 cores Piece, the model UC3825 control chips that the high speed pulse width modulation controller uses, first power amplifier, the second work( Rate amplifier, the 3rd power amplifier, the 4th power amplifier is managed using silicon carbide power MOSFET or IGBT power tubes, the carbon The breakdown voltage of SiClx power MOSFET tube or IGBT power tubes is 1200V.

Preferably, the model C2M0025D120 power tubes that the silicon carbide power MOSFET pipes use.

In summary, the utility model is by adopting the above-described technical solution, the utility model has the advantages that：

(1), the utility model makes living cells produce irreversible electrical breakdown, increases the uniformity of irreversibility point perforation, Reducing electroporation stimulates contraction of muscle caused by nerve, is ground available for treatment of solid tumor in body or vitro treatment experiment Study carefully,

(2), the utility model makes living cells produce irreversible electrical breakdown using high frequency bi-phase modulated square wave, improves electricity The uniformity of perforation effect, stimulation of the ablation pulse to nerve is reduced, reduces the contraction of muscle.Reduce and melted The influence of heat production in journey, the electrical security of irreversibility electroporation apparatus is improved, the volume of equipment is reduced, reduces equipment Weight.

Brief description of the drawings

, below will be to embodiment in order to illustrate more clearly of the example of the utility model or technical scheme of the prior art Or the accompanying drawing required in description of the prior art does and simply introduced, it should be apparent that, drawings in the following description are only this Some examples of utility model, to those skilled in the art, can be with root on the premise of creativeness is not paid Other accompanying drawings are obtained according to these accompanying drawings.

Fig. 1 is a kind of schematic diagram of the irreversible electroporation apparatus of high frequency of the utility model.

Fig. 2 is power converting circuit theory diagram of the present utility model.

Fig. 3 is the fundamental diagram of power converting circuit of the present utility model.

Fig. 4 is the fundamental diagram of isolation type high frequency conversion output circuit of the present utility model.

Fig. 5 is the control timing chart of signal controller of the present utility model.

Fig. 6 is the output pulse waveform figure of high speed pulse width modulation controller of the present utility model.

Fig. 7 is bipolar modulated pulses oscillogram of the present utility model.

Fig. 8 is the enlarged drawing of bipolar modulated pulses waveform of the present utility model.

Embodiment

Below in conjunction with the accompanying drawing in the example of the utility model, the technical scheme in the embodiment of the utility model is carried out clear Chu, it is fully described by, it is clear that described embodiment is only the utility model part of the embodiment, rather than whole realities Apply example.Based on the embodiment in utility model, those of ordinary skill in the art are obtained under the premise of creative work is not made The every other embodiment obtained, belong to the scope of the utility model protection.

Referring to Fig. 1, a kind of impulse generator of cell electroporation, it is characterised in that including power converting circuit 1, energy storage Circuit 2, isolation type high frequency conversion output circuit 3, signal controller 4, electrode 5 and power supply 6, output end and the electricity of the power supply 6 Source translation circuit 1 connects, and the power converting circuit 1 passes sequentially through accumulator 2, isolation type high frequency conversion output circuit 3 and electricity Pole 5 connects, and the signal controller 4 converts the control input of output circuit 3 with power converting circuit 1, isolation type high frequency respectively End connection.Enter electrode 5 by signal controller 4 so as to control isolation type high frequency to convert the pulse output signals of output circuit 3 Row electric discharge, the power converting circuit 1 export one or more control signal, institute for all the way, or more than two-way to be connected in parallel Isolation type high frequency conversion output circuit 3 is stated for all the way, or more than two-way to be connected in parallel, passes through the power supply all the way or more than two-way Translation circuit 1 is attached with the accumulator 2 all the way or more than two-way, the accumulator 2 with all the way or more than two-way Isolation type high frequency conversion output circuit 3 is attached, and the power converting circuit 1 exports one or more control signal control one Road or multichannel isolation type high frequency conversion output circuit 3, multichannel accumulator 2 is controlled so as to realize, the power supply 6 for+ 24V DC voltage.In the utility model, discharge and recharge is carried out to accumulator 2 by output+24V after DC-dc conversion, To ensure to export the amplitude size of pulse, accumulator 2 is that electrode 5 provides high-energy through isolation type high frequency conversion output circuit 3 Pulse, in order to improve the discharge switch in the impulse amplitude of the isolation type high frequency conversion output of output circuit 3, reduction output circuit Pressure-resistant, the raising velocity of discharge, accumulator 2 carry out the work side of the progress discharge and recharge of energy storage in parallel or series using multiple electric capacity Formula, according to the requirement of output waveform parameter, isolation type high frequency converts output circuit 3 by the energy stored in storage capacitor through electrode 5 are released to histocyte, and signal controller 4 is that impulse output circuit 3 provides trigger signal.

In the utility model embodiment, as shown in Figures 2 and 3, the power converting circuit 1 includes pulse-width modulation circuit 100th, booster circuit 101, filter circuit 102 and rectification circuit 103, power supply 6 are defeated by the signal of the pulse-width modulation circuit 100 Go out end to be connected with the control terminal of booster circuit 101, the output end of the booster circuit 101 pass sequentially through the filter circuit 102, Rectification circuit 103 is connected with accumulator 2, and the power supply 6 also connects with the input of the booster circuit 101, filter circuit 102 Connect；The pulse-width modulation circuit 100 includes PWM controller IC1, resistance R1, resistance R2, resistance R3, adjustable resistance R4, resistance R5, electric capacity C1 and electric capacity C2, the booster circuit 101 include field-effect Q1, field-effect Q2 and step-up transformer T1, the energy storage Circuit 2 includes electric capacity C3 and electric capacity C4, and the rectification circuit 103 includes diode D1 and diode D2, and the one of the resistance R1 End is connected with PWM controller IC1 oscillating discharge output end DIS, one end of the resistance R2 and PWM controller IC1 vibration The RT connections of timing resistor input, electric capacity C1 one end are connected with PWM controller IC1 vibration timing capacitor input CT, institute The other end for stating the resistance R1 other end, the resistance R2 other end and electric capacity C1 is all connected to ground, one end of the resistance R3, Adjustable resistance R4 one end, adjustable resistance R4 centre cap are all connected with the anti-phase error input INV of PWM controller IC1, can The resistance R4 other end is adjusted to be connected to ground, the first complementary output end OUTA and FET Q1 of the PWM controller IC1 grid Pole is connected, and PWM controller IC1 the second complementary output end OUTB is connected with FET Q2 grid, the FET Q1 Drain electrode be connected with one end of step-up transformer T1 primary side taps, FET Q1 source electrode respectively with PWM controller IC1 Outside cut-off signals input SD, resistance R5 one end, FET Q2 source electrode connection, the other end of the resistance R5 with Ground is connected, and the drain electrode of the FET Q2 is connected with the other end of step-up transformer T1 primary side taps, step-up transformer T1's The input with the power supply 6, filter circuit 102 are connected centre cap respectively, and the one of the step-up transformer T1 secondary taps The anode with diode D1, diode D2 negative electrode are connected respectively at end, the other end point of the step-up transformer T1 secondary taps Negative pole, electric capacity C4 positive pole not with electric capacity C3 are connected, the positive pole of the electric capacity C3 other end with resistance R3, diode respectively The input connection of D1 negative electrode, isolation type high frequency conversion output circuit 3, the negative pole of the electric capacity C4 are connected to ground, the PWM The electric capacity input SS of controller IC 1 is connected with the input/output control terminal I/O mouths of signal controller 4, the PWM controller The square-wave pulse signal that the frequency of IC1 outputs is 50kHz, the model SG3525 chips that PWM controller IC1 is used are described Signal controller 4 uses singlechip control chip.

In the utility model, as shown in figure 3, shown PWM controller IC1 electric capacity input SS ends (octal) and letter The input/output terminal I/O of number microcontroller 4 is connected, during the input/output terminal I/O output high level of this signal microcontroller 4, PWM controller IC1 is activated, and starts whole power converting circuit 1 and electric capacity is charged, as storage capacitor C3, electric capacity C4 charging, When exporting low level, stop charging to storage capacitor C3, electric capacity C4.PWM controller IC1 and its auxiliary element the resistance R2, Resistance R1 and electric capacity C1 forms pwm control circuit, when being timed electric discharge adjustment, exports the square wave arteries and veins that two-way frequency is 50kHz Punching, the width of the pulse is by the electricity on the output end E/A OUT (the 9th pin) of PWM controller IC1 internal error amplifiers The control of pressure, field-effect Q1, field-effect Q2 and step-up transformer T1 form push-pull type translation circuit, and+24V dc sources are changed into 50kHz square-wave pulse is applied to step-up transformer T1 primary side, and it is 400V, frequency to produce amplitude in step-up transformer T1 secondary Rate is 50kHz square-wave pulse, and therefore, electric capacity C3, electric capacity C4 capacitance are not less than 2200 μ F, and pressure voltage is not less than 450V.Institute State diode D1, diode D2 and electric capacity C3, electric capacity C4 forms voltage-multiplying type rectification circuit, it is by step-up transformer T1 secondary Charged after exporting impulse commutation to electric capacity C3, electric capacity C4, the series equivalent capacitance of electric capacity C3, electric capacity C4 composition is as storage capacitor The bleeder circuit that device, resistance R3 and adjustable resistance R4 are formed is used for the voltage for detecting energy-storage capacitor both ends, and feeds back to PWM controls The anti-phase error input INV (the first pin) of device IC1 internal error amplifiers processed, adjustable resistance R4 can change storage capacitor Charge value, make PWM controller IC1 internal error amplifiers inverting input INV (the first pin) keep with 5V benchmark electricity Press equal, the in-phase input end NI (second pin) and reference edge VREF the (the 16th of PWM controller IC1 internal error amplifiers Pin) it is connected；The internal error amplifier of PWM controller ICs 1 is to its in-phase input end NI (second pin) and inverting input Voltage on INV (the first pin) is compared, and is changed according to the potential difference between them inside PWM controller IC1 by mistake Voltage on the output end E/A OUT (the 3rd pin) of poor amplifier, whole circuit is set to form negative-feedback, to stablize energy storage electricity Voltage in appearance, resistance R5 are current sense resistor, and its resistance value size is 20 Ω, and it is by FET Q1, FET Q2 Source current be converted into voltage, and PWM controller IC1 outside cut-off signals input SD (the tenth pin) is sent to, with limit Maximum charging current processed.When the voltage at resistance R5 both ends reaches 1V, PWM controller IC1 is immediately by FET Q1, field-effect Pipe Q2 is turned off, and is started until next work period.

In the utility model embodiment, as shown in figure 4, isolation type high frequency conversion output circuit 3 includes high rapid pulse Wide modulation controller IC2, the first digital driver for isolating IC3, the second digital driver for isolating IC4, the first power amplifier QA, Second power amplifier QB, the 3rd power amplifier QC, the 4th power amplifier QD and isolating transformer T2, first number Word driver for isolating IC3 Enable Pin EN1, the second digital driver for isolating IC4 Enable Pin EN2 respectively with signal controller 4 The connection of input/output control terminal I/O mouths, the first complementary output end OUTA1 of the high speed pulse width modulation controller IC 2 and the One digital driver for isolating IC3 first input end VIA1, the second digital driver for isolating IC4 the second input end VIB2 Connection, the digital driver for isolating IC3's of the second complementary output end OUTB2 of the high speed pulse width modulation controller IC 2 and first Second input VIB2, the second digital driver for isolating IC4 first input end VIA2 connections；The first power amplifier QA Drain electrode, the 3rd power amplifier QC drain electrode be connected with the output end of the accumulator 2, the first power amplifier QA grids It is connected with the first digital driver for isolating IC3 the first drive output VOA1, the first power amplifier QA source electrode and ground connect Connecing, the second power amplifier QB grid is connected with the first digital driver for isolating IC3 the second drive output VOB1, and second Power amplifier QB source electrode is connected to ground, and the of the 3rd power amplifier QC grid and the second digital driver for isolating IC4 The second of one drive output VOA2 connections, the grid of the 4th power amplifier QD and the second digital driver for isolating IC4 Drive output VOB2 connections, the source electrode of the 4th power amplifier QD are connected to ground, the first power amplifier QA's The drain electrode of source electrode, the second power amplifier QB is connected with a tap of isolating transformer T2 primary sides, the 3rd power amplifier The drain electrode of QC source electrode, the 4th power amplifier QD is connected with another tap of isolating transformer T2 primary sides, the isolation transformation One tap of device T2 secondary is connected with the positive pole of electrode 5, another tap of the isolating transformer T2 secondary and bearing for electrode 5 Pole connects.

With reference to shown in Fig. 1, Fig. 2, Fig. 3 and Fig. 4, the PWM controller IC1 is according to the control instruction of output pulse, PWM controls Device IC1 processed the first complementary output end OUTA, the second complementary output end OUTB exports high level or low level respectively, exports high electricity Usually, corresponding field-effect Q1, field-effect Q2 are turned on, and when exporting low level, field-effect Q1, field-effect Q2 conducting are correspondingly cut Only.When field-effect Q1, field-effect Q2 are turned on ,+24V DC voltage passes through by diode D1, diode D2 and electric capacity C3, electricity Hold C4 and form voltage-multiplying type rectification circuit, the first power that the voltage then exported is sent into isolation type high frequency conversion output circuit 3 Amplifier QA drain electrode and the 3rd power amplifier QC drain electrode, electric capacity C3, electric capacity C4 use polypropylene film capacitor, had Good temperature stability, capacitor reliably working is ensured, noninductive characteristic, can bear very high peak-to-peak value electric current and high frequency is effective It is worth electric current.The input/output control terminal I/O of signal microcontroller 4 is simultaneously by controlling the first digital driver for isolating IC3, the Two digital driver for isolating IC4 Enable Pin EN1 and Enable Pin EN2, the input/output control terminal I/O of signal microcontroller 4 are defeated When going out high level, the first digital driver for isolating IC3, the second digital driver for isolating IC4 work, the first digital driver for isolating IC3, the second digital driver for isolating IC4 simultaneously to the first complementary output end OUTA1 of high speed pulse width modulation controller IC 2 and The pulse of second output control terminal OUTB2 outputs carries out Current amplifier, correspondingly, the first of the first digital driver for isolating IC3 The electric current (or voltage) of drive output VOA1 and the first drive output VOB1 outputs is equal to the first digital driver for isolating IC3 first input end VIA1 and the second input VIB1 electric current (or voltage), the first of the second digital driver for isolating IC4 The electric current (or voltage) of drive output VOA2 and the first drive output VOB2 outputs is equal to the second digital driver for isolating IC4 first input end VIA2 and the second input VIB2 electric current (or voltage)；First digital driver for isolating during low level IC3, the second digital driver for isolating IC4 are stopped, therefore the input/output control terminal I/O outputs of signal microcontroller 4 are high Level duration (pulse width) is exactly the width of output pulse string.

In the utility model, as shown in figure 4, the first digital driver for isolating IC3, the second digital driver for isolating The model numeral driver for isolating Si82390 chips that IC4 is used, the model that the high speed pulse width modulation controller IC 2 uses For UC3825 control chips, by signal controller 4 two driver for isolating are carried out with independent inputs control and is combined, export every From drive signal, support to be up to 5kVrms power supply MOSFET and IGBT power tube especially suitable for driving.They have high common Mould transient supression ability (100 kV/ μ s), low propagation delay (30ns), and reduce and change between temperature, aging and part, export UVLO fault detects and feedback can close two drivers automatically, thus can have high reliability, described first it is digital every Also powered from driver IC 3, the second digital driver for isolating IC4 using three independent current sources, one is+5V, and two other is +15V.The feeder ear of three independent current sources be connected to filter capacitor C2_1, filter capacitor C2_2, filter capacitor C2_3, Filter capacitor C2_4, filter capacitor C2_5 and filter capacitor C2_6, these filter capacitors all use tantalum electric capacity, for eliminating voltage Disturb caused by transition, therefore, the exportable ± 4kV of the utility model bi-directional square wave pulse, the rise time of pulse and decline Time is less than 1 μ s, and pulse width is from the μ s of 100 μ s~1000, and continuously adjustabe, pulse current peak is up to 50A.

In the utility model, as shown in figure 4, the first power amplifier QA, the second power amplifier QB, the 3rd Power amplifier QC and the 4th power amplifier QD is using silicon carbide power MOSFET pipes or IGBT power tubes, the carbonization The model C2M0025D120 power tubes that silicon power MOSFET tube uses, it is possible to provide switch at a high speed, silicon carbide power MOSFET The drain-source breakdown voltage of pipe or IGBT power tubes is 1200V, and time of switch is less than 0.1 μ s, and conducting resistance is 25m Ω, pulse Electric current is up to 250A.Isolating transformer T2 has two effects：One effect is that original edge voltage is boosted according to turn ratio； Another effect is that electrical isolation is realized between primary and secondary side.The first of the high speed pulse width modulation controller IC 2 is complementary Output end OUTA1 and the second complementary output end OUTB2 output two-way phases difference 180⁰Pwm signal, while give the first number The of the word driver for isolating IC3 first input end VIA1 and digital driver for isolating IC4 of the second input VIB1 and second One input VIA2 and the second input VIB2；Wherein, specific connected mode is by the of the first digital driver for isolating IC3 One input VIA1 is connected with the second digital driver for isolating IC4 the second input VIB2, the first digital driver for isolating IC3 the second input VIB1 is connected with the second digital driver for isolating IC4 first input end VIA2；First numeral isolation is driven Dynamic device IC3 and the first power amplifier QA drain electrode, the second power amplifier QB form a half-bridge, the second digital isolation drive Device IC4 and the 3rd power amplifier QC, the 4th power amplifier QD form another half-bridge.High speed pulse width modulation controller IC 2 The two-way pulse of output, Current amplifier and level through the first digital digital driver for isolating IC4 of driver for isolating IC3 and second Driving switch pipe (i.e. the first power amplifier QA, the second power amplifier QB, the 3rd power amplifier QC and the are distinguished after conversion Four power amplifier QD on or off), simultaneously turn on the first power amplifier QA, the second power amplifier QC, the second work( Rate amplifier QB and the 4th power amplifier QD are simultaneously turned on.When the first power amplifier QA, the second power amplifier QC are turned on When, isolating transformer T2 primary side is added into positive voltage (+Vo), i.e., Same Name of Ends is just；As the first power amplifier QB and When two power amplifier QD are turned on, it is negative plus negative voltage (- Vo), i.e. Same Name of Ends by isolating transformer T2 primary side, so as to Apply the square wave of alternation in isolating transformer T2 primary side, induce the square wave of alternation according to turn ratio on secondary, and lead to Therapeutic electrode 5 is crossed to be applied directly on target tissue.

With reference to shown in Fig. 4 and Fig. 5, the output pulse width of signal controller 4 is 100 μ s, and the cycle is 100~1000ms, width The square-wave pulse for 5V is spent, the square-wave pulse is sent into the first digital driver for isolating IC3, the second digital driver for isolating IC4 Enable Pin EN1 and Enable Pin EN2 are triggered, the first digital driver for isolating IC3, the second digital driver for isolating during high level IC4 starts working, and the first digital driver for isolating IC3, the second digital driver for isolating IC4 are stopped during low level.Such as Fig. 6 In a parts be that the first complementary output end OUTA1 output pulse widths of high speed pulse width modulation controller IC 2 are 1 μ s, pulse is all Phase is 3 μ s, and amplitude is 5V square-wave pulse.As the b parts in Fig. 6 are defeated for the second complementation of high speed pulse width modulation controller IC 2 Going out to hold OUTB2, also output pulse width is 1 μ s, and the pulse period is 3 μ s, and amplitude is 5V square-wave pulse.High speed pulse width modulation control The phase of the output pulse of device IC2 processed the first complementary output end OUTA1 and the second complementary output end differs 180 °, pulse it is dead Time is 0.5 μ s.Therefore, bipolarity pectination waveform is produced on electrode, bipolar pulse waveform is as shown in Figure 7 and Figure 8, bipolar The property pulse period is 3 μ s, wherein, positive pulse, negative pulse are respectively 1 μ s, each μ s of pulse daley 0.5.It is as shown in figure 8, this double Polar impulse waveform can not only form symmetry electroporation on cell membrane, but also can significantly reduce the stimulation to nerve Effect and the contraction of muscle.This high frequency short pulse will not cause contraction of muscle.

The preferred embodiment of utility model is the foregoing is only, it is all in this reality not to limit the utility model Within new spirit and principle, any modification, equivalent substitution and improvements made etc., it should be included in of the present utility model Within protection domain.

Claims

1. a kind of irreversible electroporation apparatus of high frequency, it is characterised in that including power converting circuit (1), accumulator (2), isolation Formula high frequency conversion output circuit (3), signal controller (4), electrode (5) and power supply (6), output end and the electricity of the power supply (6) Source translation circuit (1) connects, and the power converting circuit (1) passes sequentially through accumulator (2), isolation type high frequency conversion output circuit (3) it is connected with electrode (5), the signal controller (4) is electric with power converting circuit (1), isolation type high frequency conversion output respectively Road (3) control signal connection, the signal controller (4) so as to control isolation type high frequency convert output circuit (3) arteries and veins Rushing output signal makes electrode (5) be discharged；The power converting circuit (1) is all the way, or more than two-way to be connected in parallel, institute Isolation type high frequency conversion output circuit (3) is stated for all the way, or more than two-way to be connected in parallel, passes through the electricity all the way or more than two-way Source translation circuit (1) is attached with the accumulator (2) all the way or more than two-way, the accumulator (2) with all the way or two It is more than road isolation type high frequency conversion output circuit (3) be attached, the power supply (6) be 0~36V DC voltage, frequency For 30~100kHz.

A kind of 2. irreversible electroporation apparatus of high frequency according to claim 1, it is characterised in that the power converting circuit (1) pulse-width modulation circuit (100), booster circuit (101), filter circuit (102) and rectification circuit (103) are included, power supply (6) is logical The signal output part for crossing the pulse-width modulation circuit (100) is connected with the control terminal of booster circuit (101), the booster circuit (101) output end passes sequentially through the filter circuit (102), rectification circuit (103) is connected with accumulator (2), the electricity Input of the source (6) also with the booster circuit (101), filter circuit (102) is connected.

A kind of 3. irreversible electroporation apparatus of high frequency according to claim 2, it is characterised in that the pulse-width modulation circuit (100) PWM controller (IC1), resistance R1, resistance R2, resistance R3, adjustable resistance R4, resistance R5, electric capacity C1 and electric capacity are included C2, the booster circuit (101) include field-effect Q1, field-effect Q2 and step-up transformer T1, and the accumulator (2) includes electricity Hold C3 and electric capacity C4, the rectification circuit (103) includes diode D1 and diode D2, the resistance R1 one end are controlled with PWM Oscillating discharge output end (DIS) connection of device (IC1) processed, one end of the resistance R2 and the vibration timing of PWM controller (IC1) Resistance input (RT) is connected, and electric capacity C1 one end is connected with the vibration timing capacitor input (CT) of PWM controller (IC1), The other end of the other end of the resistance R1, the resistance R2 other end and electric capacity C1 is all connected to ground, one end of the resistance R3, Adjustable resistance R4 one end, adjustable resistance R4 centre cap error input (INV) all anti-phase with PWM controller (IC1) are even Connect, the adjustable resistance R4 other end is connected to ground, and the first complementary output end (OUTA) and the field of the PWM controller (IC1) are imitated Should pipe Q1 grid connection, the second complementary output end (OUTB) of PWM controller (IC1) is connected with FET Q2 grid, The drain electrode of the FET Q1 is connected with one end of step-up transformer T1 primary side taps, FET Q1 source electrode respectively with The source electrode connection of the outside cut-off signals input (SD), resistance R5 one end, FET Q2 of PWM controller (IC1), it is described The resistance R5 other end is connected to ground, and the other end of drain electrode Yu the step-up transformer T1 primary side taps of the FET Q2 connects Connect, the input with the power supply (6), filter circuit (102) are connected step-up transformer T1 centre cap respectively, the boosting The anode with diode D1, diode D2 negative electrode are connected respectively for one end of transformer T1 secondary taps, the step-up transformer The negative pole with electric capacity C3, electric capacity C4 positive pole are connected the other end of T1 secondary taps respectively, the positive pole of the electric capacity C3 respectively with The input connection of the resistance R3 other end, diode D1 negative electrode, isolation type high frequency conversion output circuit (3), the electric capacity C4 negative pole is connected to ground, the electric capacity input (SS) of the PWM controller (IC1) and the input/output of signal controller (4) Control terminal (I/O) mouth connects.

A kind of 4. irreversible electroporation apparatus of high frequency according to claim 3, it is characterised in that the PWM controller (IC1) The frequency of output is 50kHz square-wave pulse signal, the model SG3525 chips that the PWM controller (IC1) uses.

5. the irreversible electroporation apparatus of a kind of high frequency according to claim 3, it is characterised in that the electric capacity C3, electric capacity C4 Capacitance be not less than 2200 μ F, pressure voltage is not less than 450V.

A kind of 6. irreversible electroporation apparatus of high frequency according to claim 3, it is characterised in that the isolation type high frequency conversion Output circuit (3) includes high speed pulse width modulation controller (IC2), the first digital driver for isolating (IC3), the second numeral isolation drive Dynamic device (IC4), the first power amplifier (QA), the second power amplifier (QB), the 3rd power amplifier (QC), the 4th power are put Big device (QD) and isolating transformer T2, the Enable Pin (EN1) of the first digital driver for isolating (IC3), the second numeral isolation The Enable Pin (EN2) of driver (IC4) is connected with input/output control terminal (I/O) mouth of signal controller (4) respectively, described The first complementary output end (OUTA1) of high speed pulse width modulation controller (IC2) and the first of the first digital driver for isolating (IC3) Input (VIA1), the second input (VIB2) connection of the second digital driver for isolating (IC4), the high speed pulse width modulation control The second input (VIB2) of the second complementary output end (OUTB2) of device (IC2) processed and the first digital driver for isolating (IC3), First input end (VIA2) connection of second digital driver for isolating (IC4)；

The drain electrode of first power amplifier (QA), the drain electrode of the 3rd power amplifier (QC) and the accumulator (2) Output end connects, the first drive output of the first power amplifier (QA) grid and the first digital driver for isolating (IC3) (VOA1) connect, the source electrode of the first power amplifier (QA) is connected to ground, the grid of the second power amplifier (QB) and the first number The second drive output (VOB1) connection of word driver for isolating (IC3), the source electrode of the second power amplifier (QB) are connected to ground, The grid of 3rd power amplifier (QC) is connected with the first drive output (VOA2) of the second digital driver for isolating (IC4), The second drive output (VOB2) of the grid of 4th power amplifier (QD) and the second digital driver for isolating (IC4) is even Connect, the source electrode of the 4th power amplifier (QD) is connected to ground, source electrode, the second power of first power amplifier (QA) The drain electrode of amplifier (QB) is connected with a tap of isolating transformer T2 primary sides, the source electrode of the 3rd power amplifier (QC), The drain electrode of 4th power amplifier (QD) is connected with another tap of isolating transformer T2 primary sides, the isolating transformer T2 secondary A tap be connected with the positive pole of electrode (5), another tap of the isolating transformer T2 secondary and the negative pole of electrode (5) connect Connect.

7. the irreversible electroporation apparatus of a kind of high frequency according to claim 6, it is characterised in that the first numeral isolation is driven Dynamic device (IC3), the second digital driver for isolating (IC4) use model Si82390 chips, the high speed pulse width modulation controller (IC2) the model UC3825 control chips used, first power amplifier (QA), the second power amplifier (QB), the Three power amplifiers (QC), the 4th power amplifier (QD) is managed using silicon carbide power MOSFET or IGBT power tubes, the carbonization The breakdown voltage of silicon power MOSFET tube or IGBT power tubes is 1200V.

A kind of 8. irreversible electroporation apparatus of high frequency according to claim 7, it is characterised in that the silicon carbide power The model C2M0025D120 power tubes that MOSFET pipes use.