CN105052457B - A method of reservoir drawdown band is repaired using Bermuda grass - Google Patents
A method of reservoir drawdown band is repaired using Bermuda grass Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01B—SOIL WORKING IN AGRICULTURE OR FORESTRY; PARTS, DETAILS, OR ACCESSORIES OF AGRICULTURAL MACHINES OR IMPLEMENTS, IN GENERAL
- A01B79/00—Methods for working soil
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
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Abstract
The invention discloses a kind of methods repaired using Bermuda grass to reservoir drawdown band, are investigated and are screened by the plant to Lake Baihua reservoir drawdown band first, and filtering out Bermuda grass is to be suitble to reservoir drawdown band growing plants；Then indoor and outdoor simulated experiment is carried out to detect Bermuda grass to the adaptability of reservoir drawdown band to Bermuda grass, determines that Bermuda grass can be planted in reservoir drawdown band；Bermuda grass is finally cultivated in reservoir drawdown band and is carried out the restoration of the ecosystem of reservoir drawdown band.The present invention is had found by systematic research to be suitble in reservoir drawdown band growing plants-Bermuda grass; the long-term water logging of reservoir drawdown band and long-term exposed habitat growth can either be met; there is certain biomass again, landscape ecological and soil erosion to reservoir drawdown band have certain protective effect.
Description
Technical field
The present invention relates to a kind of reservoir drawdown band restorative procedures, and in particular to a kind of to be brought into using Bermuda grass to reservoir drawdown
The method that row is repaired, belongs to environmental protection technical field.
Background technique
Bermuda grass (Cynodonda ctylon (Linn.) Pers.) also known as Bermuda, Cynodon, perennial grass family
Plant；Well developed root system is a kind of excellent soil and slope protection plant；Vitality is strong, and breeding rapidly, is grown in flakes, and resistance to trample, seed is broadcast
Or grass blade breeding, based on vegetative propagation；With winter resistance, drought resistance, disease resistance, insect resistace, Saline alkali tolerance, resistance to shade
The characters such as property, heat resistance and antiweed performance；It is grown near village, riverbank, wasteland hillside by road, Bermuda grass is suitable more
In the perennial grass of each warm moist in the world and warm semiarid zone long-life.In China, be distributed mainly on Yellow River basin and
Its areas to the south.
The patent of invention of Publication No. CN101167423A disclose be made of Bermuda grass and English grass it is evergreen mixed
Sward level ground and its planting and establishing method are related to a kind of evergreen mixed sward level ground and its planting side being made of Bermuda grass and English grass
Method using the natural characteristic of cold-season-type plant English grass and season type plant Bermuda grass, and has using English grass
Underground rhizomes and Bermuda grass have the habit of earth's surface stolon, using the resceeding English grass kind on bermuda grass turf
Son intercuts Bermuda grass on kentucky bluegrass turf.
The patent of invention of Publication No. CN101810138A discloses a kind of breeding method of novel variety of dwarf bermudagrass,
The invention belongs to technical field of turf grass breeding, and in particular to a kind of breeding method of novel variety of dwarf bermudagrass.The present invention
It is characterized in that, by radioinduction Bermuda grass seed, carries out Field Screening, the auxiliary such as binding molecule label and Identification of Isozyme
Breeding, novel variety of dwarf bermudagrass required for obtaining in the market.
The patent of invention of Publication No. CN103772003A discloses oyster mushroom culture medium made of a kind of bermudagrass grass, special
Sign is that its major ingredient is made of the raw material of following weight：60-85 kilograms of bermudagrass grass, 12-25 kilograms of wheat bran, lime
1.5-2.5 kilograms, 1.5-2.5 kilograms of gypsum, 0.5-1.5 kilograms of brown sugar, 110-140 kilograms of water.Above-mentioned raw materials preparation method is such as
Under：A, the dry bermudagrass grass without mildew free from admixture is selected to crushed 80 meshes.
The patent of invention of Publication No. CN104429422A discloses the planting and establishing method of Bermuda grass, the planting and establishing method of Bermuda grass
Belong to agricultural technology field, more particularly, it relates to the improvement of the planting and establishing method of Bermuda grass.Present invention provides one
Kind system, the planting and establishing method of the high Bermuda grass of yield.The present invention includes the following steps：One, site preparation；Two, it breeds；Three, it conserves
Management.
The patent of invention of Publication No. CN101218873A disclose with Bermuda grass repair reservoir drawdown band and Riparian Zone by
The method for damaging the ecosystem, the method for repairing reservoir drawdown band and Riparian Zone damaged ecosystem with Bermuda grass, is related to a kind of water
The engineering technology of library falling zone and Riparian Zone restoration of the ecosystem.First screening obtains to grow in arid, thin and weak soil, also can be
Flood 1-6 a month, depth of immersion is 0-40 meters of existence, and the Bermuda grass of restoration ecosystem is used as reparation rapidly after water level falling
Plant；Area is repaired using being planted or being transplanted to after vegetative propagation or sexual propagation；Then it is conserved, it is ensured that the life in the coming year
It is long.The present invention can be promoted and recovered library falling zone and Riparian Zone ecology, and having improves riverside landscape, conserves water and soil, face source of shutting off
Pollutant and the function of improving water quality have solid soil, shore protection, absorption pollutant in water in the phase of flooding, after water level is fallen after rise
The vegetative coverage to riverbank can be quickly completed, to reach recovery and rebuild large reservoir falling zone and Riparian Zone ecological environment
Effectiveness has broad application prospects and good environmental benefit.
The patent of invention of Publication No. CN102017858A discloses Bermuda grass in big depth water logging river channel below dams and river
Application in the vegetation construction and ecological management of bank falling zone.The present invention relates to ecological environmental protections and Treatment process field, special
It is not related to application of the Bermuda grass in the vegetation construction and ecological management of big depth water logging river channel below dams and riverbank falling zone, institute
The vertical depth h that floods for stating big depth water logging river channel below dams and riverbank falling zone is 10 meters of h≤30 meter <；Through testing, dog tooth
Root is for up to 8 months being subjected to continuous flooding time.
The research of above-mentioned patent is concentrated mainly on the planting of Bermuda grass, and Bermuda grass is the oyster mushroom culture of main developing material
Base, the breeding of Bermuda grass, although CN101218873A, which discloses Bermuda grass, has repair, emphasis to reservoir drawdown band
It is mainly concentrated in and aspect is planted and conserved to Bermuda grass.Not to its systematic research.
Summary of the invention
For overcome the deficiencies in the prior art, the present invention, which provides, a kind of using Bermuda grass repairs reservoir drawdown band
Method passes through the simulated experiment of reservoir drawdown band investigation and the indoor and outdoor of Bermuda grass, it was demonstrated that Bermuda grass is to be suitble to reservoir drawdown band
One of restoration of the ecosystem plant, taken and cultivated in reparation using Bermuda grass, effectively reservoir drawdown band can be given birth to
State reparation.
To achieve the goals above, the present invention provides the following technical solutions：
A method of reservoir drawdown band is repaired using Bermuda grass, is included the following steps：
(1) it is investigated and is screened by the plant to reservoir drawdown band, filtering out Bermuda grass is to be suitble to reservoir drawdown band
Growing plants；
(2) Bermuda grass is detected by the indoor and outdoor simulated experiment to Bermuda grass to the adaptability of reservoir drawdown band, is determined
Bermuda grass can be planted in reservoir drawdown band；
(3) then Bermuda grass is cultivated in reservoir drawdown band；
(4) daily fertilizer and water management is carried out to Bermuda grass after cultivating.
In the aforementioned method repaired using Bermuda grass to reservoir drawdown band, the plant to reservoir drawdown band is carried out
Investigation and screening refer to investigation Lake Baihua falling zone plant species distribution, count its species biotype, count all sorts of flowers Lake Reservoir
Falling zone vascular plant profile map show that periodical water logging influences the distribution of plant；To its different gradient growing plants
It is counted, obtaining the first, second and third gradient has Bermuda grass growth, and coverage rate is high.
In the aforementioned method repaired using Bermuda grass to reservoir drawdown band, vegetation is in the first gradient with Bermuda grass
Based on Hemarthria compressa, the coverage of both plants is 40%~60%, and biomass is respectively 0.19~0.38kgm-2With
0.08~0.13kgm-2, which there is not other plant；Second gradient whole year water logging time was 4~6 months, was had obvious
Periodic water level fluctuation, be also based on Bermuda grass and Hemarthria compressa in the gradient, coverage increases to 85%~100%,
Biomass is respectively 0.24~0.40kgm-2With 0.12~0.17kgm-2, and there is composite family, polygonaceae species, multiplicity
Property increased, but the coverage of composite family, polygonaceae and other plant is respectively less than 5%, and biomass is again smaller than 0.05kgm-2；Third
The gradient water logging time is shorter, Hemarthria compressa in the gradient, and the coverage of Bermuda grass is 100%, and biomass also increases to 0.26~
0.53kg·m-2With 0.16~0.22kgm-2。
In the aforementioned method repaired using Bermuda grass to reservoir drawdown band, the detection Bermuda grass is to reservoir drawdown
The specific method of the adaptability of band includes the following steps：
(1) Bermuda grass is subjected to water logging experiment in summer
200 plants of the consistent Bermuda grass of growing way is planted in humus soil abundant, after plant survives, holding chamber
Outer culture 10d；The Bermuda grass cultivated is randomly divided into 2 groups：Control group and water logging group；The control group is without any place
Reason, only carries out conventional field management；Water logging group is placed in diameter 62cm, high 82cm is filled in the plastic barrel of clear water, tests initial stage
Height at the top of water surface distance plant is 0.5m, keeps inner bucket water deeply constant during experiment；
Before experiment, different disposal is respectively randomly selected and 50 plants of label, the measurement for ecological index；The 0d of experiment beginning,
It is long to measure the total branch length of the 10 plants of marked plant randomly selected, total leaf respectively by 5d, 10d, 20d, 30d；After experiment starts
0d, 4d, 8d, 20d, 30d randomly select unmarked plant healthy leaves respectively, measure the content of its chlorophyll, every kind of processing 5
A repetition；
(2) Bermuda grass is subjected to water logging experiment in winter
The Bermuda grass cultivated is randomly divided into 2 groups, every group 132 plants, control group only carries out conventional without any processing
Daily management；Water logging group is placed in diameter 62cm, high 82cm is filled in the plastic barrel of clear water, and Bermuda grass top is away from water surface elevation
0.5m；
Since October, test up to the complete water logging of 180d, using the plant without water logging as control, plant
Water discharging time is respectively T after complete water logging 30d, 60d, 90d, 120d, 150d and 180d1、T2、T3、T4、T5And T6, T1~T6Moment
Four Plants water logging group and control group respectively take 22 plants, wherein 12 plants are used to measure plant height, total number of sheets and the total leaf in ecological index
Long, 10 plants for measuring its root activity；Its biomass is measured after having measured again, the stem for randomly selecting survival measures its malonaldehyde
Content；Then the root of drying is taken out, crushes, measures the content of its total Soluble Sugar and starch；
It (3) will be by winter postflood Bermuda grass progress restoration ecosystem experiment for a long time
By the Bermuda grass cultivated carry out the complete waterlogging of different time, respectively water logging 0d, 30d, 60d, 90d, 120d,
150d and 180d, it is each to take out 10 plants for restoration ecosystem after water logging；
Before restoration ecosystem starts when 10d, cut off the withered and yellow old stem of plant above ground, on March 22nd, 2012, to water logging 0d,
Bermuda grass plant after 30d, 60d, 90d, 120d and 150d, it is each to take out 10 plants for restoration ecosystem, April 10, to water logging
The plant of 180d carries out restoration ecosystem, in restoration ecosystem 60d, have around old stem seedling turn green or old stem around there is seedling to grow,
Think that the plant underground brood body survives, it is long in 0d and 6d the measurement total branch of plant of restoration ecosystem experiment beginning, measure 60d
The biomass of plant afterwards；The root of drying is taken out, crushes, measures the content of its total Soluble Sugar and starch.
(4) growth rhythm research
Started growth rhythm research to Bermuda grass has been survived May 11, experiment start after 1d, 4d, 7d,
10d, 13d, 18d, 23d, 28d, 33d, 40d, 47d, 54d, 61d, 71d, 81d, 91d, 106d, 121d, 141d measure 30 at random
Total branch length, the length of the 3rd, 4 young leaves of total number of sheets and top in the ecological index for the Bermuda grass plant that strain has marked, and it is each
10 plants of measurement biomass of random acquisition, the root of drying and stem are separated and are crushed, measure its total Soluble Sugar and content of starch and
Root, stem, leaf the content of nitrogen and phosphorous.
In the aforementioned method repaired using Bermuda grass to reservoir drawdown band, the measuring method packet of the ecological index
It includes：
Plant height：The height of plant above ground first section to top phyllopodium is surveyed with measuring tool；
Total number of sheets：Manually count the plant above ground whole number of sheets；
Total leaf is long：The length that the green portion of plant whole blade is measured with measuring tool, averages；
Total branch is long：The Branches of Different Orders length summation of plant above ground one section or more is measured with measuring tool；
Biomass：The whole strain of plant is cleaned, 103 DEG C of water-removing 20min are weighed in 80 DEG C of baking 3d to constant weight with counter balance
Its weight.
In the aforementioned method repaired using Bermuda grass to reservoir drawdown band, the measuring method of the chlorophyll is：It adopts
Collect plant healthy leaves, remove both ends of the blade, remove vein broad in the middle, with acetone-ethanol mixed liquor method measurement chlorophyll
Content；
The measuring method of the total Soluble Sugar is：The root or stem for weighing crushing add 80% dehydrated alcohol of 3ml, boiling water
30min is extracted in bath, and centrifuging and taking supernatant extracts 2 times repeatedly, is settled to 10ml, the measurement for total Soluble Sugar；
The measuring method of the starch is：Add 4.6mol/L high chlorine with the residue of measurement total Soluble Sugar extracting solution filtering
Sour 2ml, boiling water bath 15min add pure water to 10ml, and centrifugation takes supernatant, so extracts again 1 time, with pure water residue 2 times,
Centrifuging and taking supernatant, supernatant is merged, and is settled to 50ml, the measurement for starch；
The measuring method of the root activity is：Plant healthy leaves are acquired, old leaf and top two panels young leaves is avoided, goes
Except both ends of the blade, vein broad in the middle is removed, measures root activity with TTC method；
The measuring method of the malonaldehyde is：The stem for taking 0.5g Bermuda grass to survive at random, adds 10% trichloroacetic acid of 5ml,
Grinding, centrifugation, isolates supernatant；2ml supernatant is taken, 0.5% thiobarbituricacidα- of 2ml is added to mix, boiling water bath 20min is cold
But, it is centrifuged, measures its OD value；
The measuring method indophenol blue colorimetry of the nitrogen content；
The method for measuring phosphor content molybdenum antimony resistance colorimetric method.
The beneficial effects of the invention are as follows：
The present invention has carried out long-term investigation and research by the vegetation to Lake Baihua, and filtering out Bermuda grass is to be suitble to reservoir
Falling zone growing plants；Then the present invention passes through the simulated experiment of the indoor and outdoor to Bermuda grass, it was demonstrated that Bermuda grass is suitable
Bermuda grass is planted on reservoir drawdown band by one of restoration of the ecosystem plant of reservoir drawdown band, can be preferably to reservoir drawdown
Band carries out restoration of the ecosystem.Bermuda grass can either meet the long-term water logging of reservoir drawdown band and long-term exposed habitat growth, have again
There is certain biomass, the landscape ecological and soil erosion to reservoir drawdown band have certain protective effect.Bermuda grass can be effective
Ground retention storage suspended matter cuts down water nutrition substance, has good shore protection and landscape value.
Detailed description of the invention
Figure 1A is the relative growth rate of Bermuda grass branch under different water logging time-triggered protocols
Figure 1B is the relative growth rate of Bermuda grass blade under different water logging time-triggered protocols
Fig. 1 C is the variation of Bermuda grass chlorophyll a under different water logging time-triggered protocols
Fig. 1 D is the variation of Bermuda grass chlorophyll b under different water logging time-triggered protocols
Fig. 2A is Bermuda grass branch amount under different time waterlogging
Fig. 2 B is that the total branch of Bermuda grass is long under different time waterlogging
Fig. 2 C is Bermuda grass total number of sheets under different time waterlogging
Fig. 2 D is that the total leaf of Bermuda grass is long under different time waterlogging
Fig. 2 E is Bermuda grass total biomass under different time waterlogging
Fig. 3 A is mda content in Bermuda grass tissue under different time waterlogging
Fig. 3 B is soluble-carbohydrate concentration in Bermuda grass under different time waterlogging
Fig. 3 C is starch concentration in Bermuda grass under different time waterlogging
Fig. 3 D is Bermuda grass root activity under different time waterlogging
Fig. 4 A is Bermuda grass relative growth rate after different time waterlogging
Fig. 4 B is the total biomass after different waterloggings after Bermuda grass water outlet growth 2 months
Fig. 4 C is the Bomass allocation situation after different waterloggings after Bermuda grass water outlet growth 2 months
Fig. 4 D is the carbohydrate content after different waterloggings after Bermuda grass water outlet growth 2 months
Fig. 4 E is the content of starch after different waterloggings after Bermuda grass water outlet growth 2 months
Fig. 4 F is the total sugar content after different waterloggings after Bermuda grass water outlet growth 2 months
Fig. 5 A is the Bermuda grass long matched curve of total branch during the growth process
Fig. 5 B is the matched curve of Bermuda grass total number of sheets during the growth process
Fig. 5 C is the Bermuda grass long matched curve of total leaf during the growth process
Fig. 5 D is the matched curve of Bermuda grass total biomass during the growth process
Fig. 5 E is the accumulation law of Bermuda grass carbohydrate during the growth process
Fig. 5 F is Bermuda grass carbohydrate distribution situation during the growth process
Fig. 6 A be Bermuda grass during the growth process in each organ biomass variation
Fig. 6 B be Bermuda grass during the growth process in each organ nitrogen content variation
Fig. 6 C be Bermuda grass during the growth process in each organ phosphorus content variation
Fig. 6 D be Bermuda grass during the growth process in each organ N bioaccumulation coefficient
Fig. 6 E is Bermuda grass nitrogen released rate in each organ during the growth process
Fig. 6 F is Bermuda grass phosphorus burst size in each organ during the growth process
Fig. 7 is Bermuda grass growing state after the plantation of the falling zone of Guizhou all sorts of flowers Lake Reservoir
Specific embodiment
A kind of method that reservoir drawdown band is repaired using Bermuda grass of embodiment
(1) plant of reservoir drawdown band is investigated and is screened
The investigation and screening of 1 reservoir drawdown band vascular plant
1.1 investigation method
5~August in 2011, in December, 2011 and in August, 2013, investigation Lake Baihua falling zone plant species distribution, statistics
Its species biotype.
1.2 Lake Baihua reservoir drawdown band vascular plant compositing characteristics
Using Guiyang City, Guizhou Province Lake Baihua reservoir drawdown band as research object, survey region species are abundant, share dimension Guan Zhi
147 kinds, it is subordinate to 49 sections 116 category.Survey region is amounted to 43 sections 110 and belongs to 140 kinds, accounted for total Number of Species based on angiosperm
95.24%；And pteridophyte, there are 6 sections 6 to belong to 7 kinds, only accounts for the 4.76% of sum.Angiosperm is based on dicotyledon, altogether
It counts 36 sections 79 and belongs to 103 kinds, account for the 70.01% of sum；Monocotyledon, 7 sections 31 belong to 37 kinds, account for the 25.23% of sum.In addition,
Only occur 26 section of Ke You for belonging to one kind in survey region, account for respectively Superfamily number 53.06%, it is total belong to number 22.41%,
The 17.69% of total Number of Species；Composite family kind number is most, there is 29 kinds of 21 category, accounts for always belong to number 18.10%, total Number of Species respectively
19.73% (table 1A).
Table 1A Lake Baihua falling zone vascular plant section belongs to kind of a feature
1.3 Lake Baihua reservoir drawdown band vascular plant life-form spectrums
Whole vascular plants are divided into phaenerophytes (P), hemicryptophyte (H), ground by Raunkiaer classification method
Bud plant (G) and annual plant (T).Survey region plant life form 85 kinds, accounts for total Number of Species based on annual plant
57.82%；Phaenerophytes is minimum, 12 kinds, accounts for 8.16% (table 1B) of sum.Monocotyledon is in the majority with annual plant,
It secondly is geophyte respectively；Dicotyledon is secondly phaenerophytes, the plant of ground bud respectively based on annual plant
Object, geophyte are minimum (table 1B).What the low elevation region in Lake Baihua falling zone was mainly distributed is annual plant and ground bud
Plant, phaenerophytes are then mainly distributed on elevation region.This shows that periodical water logging influences the distribution of plant.
Table 1B Lake Baihua falling zone vascular plant life-form spectrum
1.4 Lake Baihua reservoir drawdown band vascular plant profile features
The species that survey area occurs belong to 15 profiles (table 1C), first three profile of species quantity ranking is respectively
The world distribution, it is general the torrid zone and north temperate zone profile, have 52 kinds, 36 kinds, 26 kinds respectively, account for respectively total species number 35.37%,
24.49%, 24.49%, the 77.55% of total species number is accounted for altogether.Eurasian and South America temperate zone Disjunct distribution, the distribution of temperate zone Asia, in
State-Japan only has a kind.
Table 1C Lake Baihua reservoir drawdown band vascular plant profile map
Note：In areal type number：1 is distributed for the world, and 2 be the general torrid zone, and 2-1 is Tropical Asia, Oceania (to new west
It is blue) and in, South America (or Mexico) Disjunct distribution, 3 be Tropical Asia and tropical America Disjunct distribution, and 6 be Tropical Asia to hot
Band Africa, 7 be Tropical Asia (India, Malaysia), and 8 be north temperate zone, and 8-4 is north temperate zone and south temperate zone Disjunct distribution, 8-5 are
Eurasian and South America temperate zone Disjunct distribution, 9 are interrupted for East Asia and North America continent, and 10 be old world temperate zone, and 11 are distributed for temperate zone Asia, and 12
It is distributed for Mediterranean, West Asia to the Central Asia, 14 for East Asia, 14-2 China-Japan.
Lake Baihua reservoir drawdown band species diversity is in medium level, disappears higher than the white falling zone Jia Xi and west Qinling mountains area
Band is fallen, Three Gorges Reservoir is lower than.Due to being influenced by the different water logging times, Lake Baihua river channel below dams dominant plant is constituted
Also there is notable difference.For vegetation based on Bermuda grass and Hemarthria compressa, the coverage of both plants is 40% in first gradient
~60%, biomass is respectively 0.19~0.38kgm-2With 0.08~0.13kgm-2, which, which there is no, there is it
His plant.Second gradient whole year water logging time was 4~6 months, there is a fluctuation of more apparent periodic water level, in the gradient still
It is so that coverage increases to 85%~100% based on Bermuda grass gramineous and Hemarthria compressa, biomass is respectively 0.24~
0.40kg·m-2With 0.12~0.17kgm-2, and there are the species such as composite family, polygonaceae, diversity slightly increased, but
The coverage of composite family, polygonaceae and other plant is respectively less than 5%, and biomass is again smaller than 0.05kgm-2.The 3rd gradient water logging time
Shorter, Hemarthria compressa in the gradient, the coverage of Bermuda grass is substantially 100%, and biomass also increases to 0.26~0.53kg
m-2With 0.16~0.22kgm-2.3rd gradient not only occurs the plants such as the composite family of the second gradient, polygonaceae, has also appeared crow
Fern, ramie, the biggish plant of the single plants Plant weight such as fruit and Salix variegata, although these plant individual plants weight are larger,
Coverage and biomass are in falling zone but very little.And the other kinds of plant that upper table is listed only sends out in the investigation of falling zone
Existing, the range of distribution is not wide, while also without biggish coverage and biomass.It can thus be seen that falling zone plant
Distribution is influenced more obviously by water logging, and the group of plant is relatively simple, and coverage and biomass are lower, shows Lake Baihua and disappears
The Ecological Fragility settled in an area.Wherein Cynodon is in one of the main growing plant of this falling zone.
It is adapted to long-term water logging and exposed environment in order to verify Bermuda grass, the present invention continues to study as follows.
(2) Bermuda grass is detected to the adaptability of reservoir drawdown band by the indoor and outdoor simulated experiment to Bermuda grass
1, Bermuda grass is subjected to water logging experiment in summer
1.1 experimental method
200 plants of the consistent Bermuda grass of growing way is planted in humus soil abundant, after plant survives, holding chamber
Outer culture 10d；The Bermuda grass cultivated is randomly divided into 2 groups：Control group and water logging group；The control group is without any place
Reason, only carries out conventional field management；Water logging group is placed in diameter 62cm, high 82cm is filled in the plastic barrel of clear water, tests initial stage
Height at the top of water surface distance plant is 0.5m, keeps inner bucket water deeply constant during experiment；
Before experiment, different disposal is respectively randomly selected and 50 plants of label, the measurement for ecological index；The 0d of experiment beginning,
It is long to measure the total branch length of the 10 plants of marked plant randomly selected, total leaf respectively by 5d, 10d, 20d, 30d；After experiment starts
0d, 4d, 8d, 20d, 30d randomly select unmarked plant healthy leaves respectively, measure the content of its chlorophyll, every kind of processing 5
A repetition；
Wherein, the measuring method of plant height, total number of sheets, total leaf length and biomass is respectively：
Plant height：The height of plant above ground first section to top phyllopodium is surveyed with measuring tool；
Total number of sheets：Manually count the plant above ground whole number of sheets；
Total leaf is long：The length that the green portion of plant whole blade is measured with measuring tool, averages；
Biomass：The whole strain of plant is cleaned, 103 DEG C of water-removing 20min are weighed in 80 DEG C of baking 3d to constant weight with counter balance
Its weight.
The measuring method of total Soluble Sugar is：The root or stem for weighing crushing add 80% dehydrated alcohol of 3ml, boiling waterbath
30min, centrifuging and taking supernatant extract 2 times repeatedly, are settled to 10ml, the measurement for total Soluble Sugar.
The measuring method of starch is：Add 4.6mol/L perchloric acid 2ml with the residue of measurement total Soluble Sugar extracting solution filtering,
Boiling water bath 15min adds pure water to 10ml, and centrifugation takes supernatant, so extracts again 1 time, with pure water residue 2 times, centrifuging and taking
Supernatant merges supernatant, is settled to 50ml, the measurement for starch.
The measuring method of root activity is：Plant healthy leaves are acquired, old leaf and top two panels young leaves are avoided, remove blade
Both ends remove vein broad in the middle, measure root activity with TTC method.
The measuring method of malonaldehyde is：The stem for taking 0.5g Bermuda grass to survive at random, adds 10% trichloroacetic acid of 5ml, grinds,
Centrifugation, isolates supernatant；2ml supernatant is taken, 0.5% thiobarbituricacidα- of 2ml is added to mix, boiling water bath 20min is cooling, from
The heart measures its OD value.
The measuring method of the chlorophyll is：Plant healthy leaves are acquired, both ends of the blade is removed, removes leaf broad in the middle
Arteries and veins, with the content of acetone-ethanol mixed liquor method measurement chlorophyll.
1.2 experiment conclusion
1.2.1 the relative growth rate of branch
Under the conditions of water logging, Bermuda grass shoot growth is slow, and growth rate is integrally substantially less than control group (p<0.05, n=
10) (Figure 1A).From Figure 1A it can be seen that the relative growth rate of Bermuda grass water logging in 30 days, Bermuda grass water logging in the 5th day and non-water
It floods relative growth rate difference less, started at the 10th day, there is negative growth in the growth rate of Bermuda grass, and at the 20th, water logging
Bermuda grass growth rate is almost 0, and at the 30th day, negative growth occurred in the Bermuda grass of water logging.Thus it sees, Bermuda grass passes through water logging
Non- water logging comparative study is tested by prolonged water logging, and the Bermuda grass of water logging is not dead, is only increased and is slowed down.
1.2.2 the relative growth rate of leaf
Water logging significantly inhibits the growth of Bermuda grass leaf, and growth rate is lower than control group；The growth rate of 5d moment leaf is significant
Higher than control group, this may be the sprouting (Figure 1B) of the leaf bud promoted due to water logging.
1.2.3 the variation of chlorophyll a
When blade water logging, the content of plant chlorophyll a is integrally lower than control group；The content of Bermuda grass chlorophyll a is in raising
Variation tendency (Fig. 1 C).
1.2.4 the variation of chlorophyll b
When blade water logging, Bermuda grass content of chlorophyll b is substantially less than control group (p<0.05, n=10)；Bermuda grass chlorophyll
B content is in raising trend (Fig. 1 D).
1.2.5 the variation of chlorophyll a/b
With blade whether the related variation there are also plant leaf blade inner chlorophyll a/b ratio of water logging.When blade water logging, dog tooth
The reduction rate of root chlorophyll a is lower than chlorophyll b, and leading to the ratio of its chlorophyll a/b is in that raising becomes.
2, Bermuda grass is subjected to water logging experiment in winter
2.1 experimental method
The Bermuda grass cultivated is randomly divided into 2 groups, every group 132 plants, control group only carries out conventional without any processing
Daily management；Water logging group is placed in diameter 62cm, high 82cm is filled in the plastic barrel of clear water, and Bermuda grass top is away from water surface elevation
0.5m；
Since October, test up to the complete water logging of 180d, using the plant without water logging as control, plant
Water discharging time is respectively T1, T2, T3, T4, T5 and T6, T1~T6 after complete water logging 30d, 60d, 90d, 120d, 150d and 180d
Moment Four Plants water logging group and control group respectively take 22 plants, wherein 12 plants for measure plant height in ecological index, total number of sheets and
Total leaf is long, and 10 plants for measuring its root activity；Its biomass is measured after having measured again, randomly select survival stem measure its third
Dialdehyde content；Then the root of drying is taken out, crushes, measures the content of its total Soluble Sugar and starch.
2.2 experiment conclusion
2.2.1 ecologic adaptation
Under the conditions of complete water logging, Bermuda grass does not generate new branch, and total branch number and total branch length do not dramatically increase,
60d~120d is substantially less than control group (p<0.01, n=12) (Fig. 2A and Fig. 2 B).The growth of Bermuda grass leaf is consumingly pressed down
System, total number of sheets and total leaf are long whole lower than control group；When 30d~150d, total number of sheets and total leaf length maintain always reduced levels,
When 180d, or even it is reduced to 0 (Fig. 2 C and Fig. 2 D).Bermuda grass biomass is integrally in decreasing trend, substantially less than control group (p<
0.01, n=12)；With continuing for water logging, the difference of biomass and control group is gradually increased, when 30d phase difference 0.16g, 180d
Differ 0.33g, only 56.07% (Fig. 2 E) of control group.
2.2.1 physiological adaptability
Bermuda grass root total Soluble Sugar and content of starch are integrally in decline, whole and control group significant difference (p<0.01,
N=5)；The total Soluble Sugar of high-content when 90d is conducive to improve the ability that plant resists cold climate.Root activity is integrally in
Decreasing trend, with control group significant difference (p<0.01, n=5)；When water logging 120d, root activity reaches minimum, this may be
Since the improvements of weather are conducive to the raising (Fig. 3 A, Fig. 3 B, Fig. 3 C, Fig. 3 D) of plant root vigor.
It 3, will be by winter postflood Bermuda grass progress restoration ecosystem experiment for a long time
3.1 experimental method
By the Bermuda grass cultivated carry out the complete waterlogging of different time, respectively water logging 0d, 30d, 60d, 90d, 120d,
150d and 180d, it is each to take out 10 plants for restoration ecosystem after water logging；
Before restoration ecosystem starts when 10d, cut off the withered and yellow old stem of plant above ground, on March 22nd, 2012, to water logging 0d,
Bermuda grass plant after 30d, 60d, 90d, 120d and 150d, it is each to take out 10 plants for restoration ecosystem, April 10, to water logging
The plant of 180d carries out restoration ecosystem, in restoration ecosystem 60d, have around old stem seedling turn green or old stem around there is seedling to grow,
Think that the plant underground brood body survives, it is long in 0d and 6d the measurement total branch of plant of restoration ecosystem experiment beginning, measure 60d
The biomass of plant afterwards；The root of drying is taken out, crushes, measures the content of its total Soluble Sugar and starch.
3.2 experiment conclusion
3.2.1 survival rate
After complete waterlogging 30d, 60d, 90d, 120d, 150d, 180d, Four Plants maintain high survival
Rate has reached 100%, does not have difference (table 2A) with the plant of non-water logging.This illustrates Lake Baihua falling zone plant Bermuda grass, hollow
Alternanthera, Hemarthria compressa and Bermuda grass have been already adapted to water logging environment, smoothly spend the very long water logging phase, and keep higher survival
Rate.
The survival rate of Bermuda grass after the complete waterlogging of table 2A different time
3.2.2 restoration ecosystem ability
Water logging has no significant effect the restoration ecosystem of Bermuda grass；Short-term water logging can promote Bermuda grass restoration ecosystem rate
It improves；Long-time water logging brings adverse effect to Bermuda grass restoration ecosystem；T1Moment is discharged plant growth rate and is higher than non-water logging
Plant, also above other moment water outlet plant, this may be since the rise of in March, 2012 temperature subtracts to a certain extent
Weak water logging bring adverse effect (Fig. 4 A).
3.2.3 biomass
After the complete waterlogging of different time, the biomass of Bermuda grass restoration ecosystem accumulation is below non-water logging plant.Water
Flooding influences significantly the accumulation of biomass after Bermuda grass water, whole to be lower than non-water logging plant；T6The moment plant of water outlet is significant
Lower than 77.93% (Fig. 4 B).Flood T1~T3The biomass that moment water outlet restoration ecosystem is accumulated is generally relatively low, this may be with plant
Temperature is lower related after water outlet.This show water outlet after phytomass accumulation be also discharged by plant after climatic effect.
3.2.4 biomass allocation
Different time waterlogging influences the distribution of restoration ecosystem stage biomass after water outlet.Bermuda grass biomass is in each structure
The distribution situation of part is stem>Root>Leaf undergoes the specific gravity of the Plant Leaf of water logging to be above non-water logging plant (Fig. 4 C).Stem institute accounting
Example is above root leaf, this breeding for being conducive to plant is lived；And the raising of leaf ratio, facilitating plant improves photosynthesis, product
Tire out more carbohydrate.
3.2.5 carbohydrate
Plant is discharged back root part soluble-carbohydrate significant difference (p<0.05, n=10) (Fig. 4 D).Bermuda grass root
The starch of accumulation increases with the water logging time and is increased, content of starch significant difference (p between each moment water outlet plant<0.05, n=10)
(Fig. 4 E).With continuing for water logging, Bermuda grass is influenced significantly by water logging, and the plant root total Soluble Sugar of waterlogging is significantly low
In control group (Fig. 4 F).
4, in restoration ecosystem its growth rhythm research
4.1 experimental method
Started growth rhythm research to Bermuda grass has been survived May 11, experiment start after 1d, 4d, 7d,
10d, 13d, 18d, 23d, 28d, 33d, 40d, 47d, 54d, 61d, 71d, 81d, 91d, 106d, 121d, 141d measure 30 at random
Total branch length, the length of the 3rd, 4 young leaves of total number of sheets and top in the ecological index for the Bermuda grass plant that strain has marked, and it is each
10 plants of measurement biomass of random acquisition, the root of drying and stem are separated and are crushed, measure its total Soluble Sugar and content of starch and
Root, stem, leaf the content of nitrogen and phosphorous.
Wherein, the long measuring method of total branch is：The Branches of Different Orders of plant above ground one section or more is measured with measuring tool
Length summation；
Full N measurement measures nitrogen content with indophenol blue colorimetry (LY/T 1269-1999), and the full P measurement of plant is anti-with molybdenum antimony
Colorimetric method (LY/T1270-1999).
4.2 experiment conclusion
4.2.1 on-site inspection
On-site inspection discovery, the Bermuda grass that Lake Baihua falling zone screens are patch ground sprawl growth, and maximum height does not surpass
Cross 5cm.Therefore, when we record growing height curve, the sum of each branch length of Bermuda grass is chosen as record object.Experiment note
It records as follows：
Table 2B Bermuda grass grows number of days and height
4.2.2 the fitting of Bermuda grass growth curve
Using the growth number of days of Bermuda grass as variable, each branch length summation is dependent variable, uses line respectively using SPSS18.0
Property type, logarithmic, shape reciprocal, it is secondary, three times, compound, power, S, growth, index, Logistic be fitted, find secondary letter
Several and cubic function fitting degree R is equal to 0.974 (significance is less than 0.01), but parameter b3 in cubic function equation
=1.814 × 10-6, parameter is too small, has little significance, so the growth curve of Bermuda grass uses quadratic function equation：Y=-
0.001x2+0.379x+9.201。
Table 2C Bermuda grass growth curves model summarizes and parameter Estimation
Dependent variable：Total branch length
Independent variable is made a living long number of days.
4.2.3 Bermuda grass grows the curve matching of each morphological index
Quadratic term curve and fitting effect of cube curve to total shoot growth feature of Bermuda grass are preferable, significance probability
Less than 0.01.Bermuda grass cube curve model fitting effect is better than quadratic term curve model, RS 0.980；Its growth model is y
=2.901 × 10-5x3-0.006x2+ 0.652x+6.88 (Fig. 5 A).
Quadratic term curve, cube curve, logarithmic curve and power function model can preferably be fitted the increase of Bermuda grass blade
Feature, fitting effect are power function model (RS=0.781)>Cube curve (RS=0.732)>Logarithmic curve (RS=0.674)>
Quadratic term curve (RS=0.626), it is y=8.849x that plant leaf, which increases equation,0.223。
The growth of cube curve model fitting Bermuda grass young leaves and the effect of time Relationship are better than other models, significantly
For property probability less than 0.01, the RS being fitted is respectively 0.92.Bermuda grass relational expression is y=6.125 × 10-6x3-0.001x2-
0.016x+6.904 (Fig. 5 C).
Quadratic term curve can be fitted Bermuda grass total biomass accumulation characteristic well, and RS is 0.942 respectively, and model is y
=5.743 × 10-5x2+ 0.006x -0.008 (Fig. 5 D).
4.2.4 Bermuda grass grows the curve matching of each physical signs
Bermuda grass root Soluble adhesion molecule is integrally on a declining curve, and fluctuating range is larger, and Four Plants can when 141d
Dissolubility total sugar content approaches a value (Fig. 5 E).
Bermuda grass root is constantly accumulating starch；Within entire growth period, root starch content is constantly fluctuated, dog tooth
The fluctuating range of root is larger (Fig. 5 F).
4.2.5 biomass (dry weight) variation of each organ of Bermuda grass
The biomass in each organ each period is all stem maximum it can be seen from Fig. 6 A, and root and leaf are smaller, and the biomass of stem is flat
It is 10 times or more of leaf and root biomass.It is all more slow in the biomass growth rate of 0~30d root, stem and leaf；30~
150d increases comparatively fast, and this is mainly due to caused by stem rapid growth, leaf and root are smaller to the contribution of total biomass.150d with
Afterwards, the root of plant and the biomass of stem, which are advanced the speed, starts to reduce, and the Leaf biomass of plant is begun to decline, this may be due to
Into autumn, the blade of plant, which starts to occur decaying, or death is withered and fallen causes.
4.2.6 variation of the nitrogen phosphorus total amount in each organ of Bermuda grass
Nitrogen and phosphorus content total amount is all stem maximum in each period in Bermuda grass stem it can be seen from Fig. 6 B and 6C, Ye Hegen's
Content is smaller, and nitrogen and phosphorus content accounts for 80% or so of entire plant total amount in each period in stem.Plant N total amount and N in root, stem are total
The correlation of amount is respectively 0.955**, 0.999**, and with N total amount in leaves of plants without significant correlation.Plant P total amount and root,
The correlation of P total amount is respectively 0.992**, 0.999** in stem, and with P total amount in leaves of plants also without significant correlation.But due to
N, P total amount content are lower in plant roots, so the content height of N, P total amount in stem plays decision to whole plant N, P total amount
The effect of property.Meanwhile for whole plant, the fit equation of N, P total amount is respectively y=in plant cultivating number of days and plant
0.026x2+ 0.502 (R=0.983, P<And y=0.291e 0.01)0.018x(R=0.920, P<0.01).Annual July~
October, plant are most fast to the absorption rate of N, P, and plant obviously slows down the absorption rate of N, P after October.
4.2.7 enrichment of each organ of plant to N, P
The soil that laboratory serike is used to do plant growth curve is taken from Lake Baihua falling zone.Battalion in the soil
It supports substance and micronutrient levels is shown in Table 2D.
Nutriment in table 2D soil
Assuming that constant (the N content 3.846mg/g of N, P concentration in the growth course of plant, in soil；P content is
1.36mg/g), each organ bioaccumulation index of plant during the growth process is calculated.
Bioaccumulation index (BAF)=MPlant/MSoil
In formula, MPlantFor the levels of N, P element in plant organ, MSoilContain for the concentration of N, P element in the soil
Amount.
It can be seen from Fig. 6 D in incipient 30 days, root, stem, leaf start decline sharply to the accumulation factor of nitrogen phosphorus,
Then in June~September, the BAF fall off rate of each organ slows down, finally slowly rises in September~November.Due to plant ratio early period
It is shorter and smaller, it is not strong to the absorbability of N, P, so that the BAF of each organ sharply declines to supply growth.May is long to September plant
Greatly, the absorbability in soil is enhanced, but plant the absorption rate of N, P is increased than biomass in growing process and
Caused dilution rate is slow, so will lead to the cumulative speed of N in each plant organ of plant reduces reduced rate.September part
After, as the advancing the speed for biomass of plant starts to slow down, plant is still constantly absorbing N, P nutriment, and can make
BAF increases in each organ.Especially be worth we note that, BAF later period advancing the speed in leaf is greater than root and stem, this
May be due to the Major Nutrient organ that leaf is plant, while it may also is that plant leaf segments start to bloom or as a result,
A large amount of nutrient, root and stem is needed to reinforce the transport degree of leaf nutrition substance.
4.2.8 release of the Bermuda grass to N, P
In November, 2011, dead each 2kg of two kinds of whole strains of plant of Bermuda grass was left in the case where Lake Baihua falling zone acquires natural conditions
Laboratory is taken back on the right side.Plant sample is washed with deionized water surface soil, the moisture 6h on surface is air-dried at shady and cool ventilation, often
It is separated that kind of plant presses root, stem, leaf, claims to obtain fresh weight.Every kind of sample root, stem, each 50g of leaf are then chosen, be fitted into decomposition band and is done
Upper label, be put into December, 2012 Lake Baihua falling zone wheat Xihe River bank it is biological floating bed under, underwater penetration 1m.From investment
Start test in lake to count for the 0th day, takes out and be soaked in respectively according to 1d, 3d, 7d, 15d, 30d, 60d, 90d, 120d, 150d
Each one bag of the root, stem and leaf of various plants in lake, take back the measurement that laboratory carries out N, P total amount.
It can be seen from Fig. 6 E and Fig. 6 F in 150 days, leaves of plants can discharge release 70% or more nutritive salt,
And root, stem can only release the nutritive salt less than 50%, and rate of release leaf>Stem>Root.Simultaneously it can be seen that at 1~30 day
Interior, the rate of release of each organotrophy's salt is slower, and with the lengthening of soaking time, the rate and total amount of Plant emission start to increase
Add.
By the research of above-mentioned aspect, the results showed that, Bermuda grass still has vitality after long-term water logging, by one
Plant growth condition is good after the restoration ecosystem of section time, it can be seen that Bermuda grass can adapt to the long-term water of reservoir drawdown band
It floods with long-term exposed growing environment, the acceptable quick restoration ecosystem after long-term water logging, and can quickly adapt to
Exposed growing environment, growth potential is preferable, can be planted in reservoir drawdown band.
Finally Bermuda grass is cultivated in reservoir drawdown band；And daily rich water pipe is carried out to Bermuda grass after cultivating
Reason is obvious to the repair of reservoir through cultivation after a period of time.
Bermuda grass plays an important role in the restoration of the ecosystem of reservoir drawdown band at present, in real life, dog
Root of the tooth has carried out experimental planting (see Fig. 7) in the falling zone of Guizhou all sorts of flowers Lake Reservoir, to Lake Baihua reservoir drawdown band
Repair is obvious, while reducing water and soil soil erosion, has beautified environment, is conducive to Guizhou Lake Baihua reservoir ecosystem
It is steady in a long-term.
Claims (4)
1. a kind of method repaired using Bermuda grass to reservoir drawdown band, which is characterized in that include the following steps：
(1) it is investigated and is screened by the plant to reservoir drawdown band, filtering out Bermuda grass is to be suitble to reservoir drawdown band growth
Plant；
(2) Bermuda grass is detected by the indoor and outdoor simulated experiment to Bermuda grass to the adaptability of reservoir drawdown band, determines dog tooth
Root can be planted in reservoir drawdown band；
(3) then Bermuda grass is cultivated in reservoir drawdown band；
(4) daily fertilizer and water management is carried out to Bermuda grass after cultivating；
The detection Bermuda grass includes the following steps the specific method of the adaptability of reservoir drawdown band：
(1) Bermuda grass is subjected to water logging experiment in summer
It is planted in 200 plants of the consistent Bermuda grass of growing way in humus soil abundant, after plant survives, is trained outside holding chamber
Support 10d；The Bermuda grass cultivated is randomly divided into 2 groups：Control group and water logging group；The control group is without any processing, only
Carry out conventional field management；Water logging group is placed in diameter 62cm, high 82cm is filled in the plastic barrel of clear water, experiment the initial stage water surface away from
It is 0.5m from the height at the top of plant, keeps inner bucket water deeply constant during experiment；
Before experiment, different disposal is respectively randomly selected and 50 plants of label, the measurement for ecological index；0d, 5d of experiment beginning,
It is long to measure the total branch length of the 10 plants of marked plant randomly selected, total leaf respectively by 10d, 20d, 30d；Experiment start after 0d,
4d, 8d, 20d, 30d randomly select unmarked plant healthy leaves respectively, measure the content of its chlorophyll, 5 weights of every kind of processing
It is multiple；
(2) Bermuda grass is subjected to water logging experiment in winter
The Bermuda grass cultivated is randomly divided into 2 groups, every group 132 plants, control group only carries out conventional day without any processing
Often management；Water logging group is placed in diameter 62cm, high 82cm is filled in the plastic barrel of clear water, and Bermuda grass top is away from water surface elevation 0.5m；
Since October, test up to the complete water logging of 180d, using the plant without water logging as control, plant is complete
Water discharging time is respectively T after water logging 30d, 60d, 90d, 120d, 150d and 180d1、T2、T3、T4、T5And T6, T1~T6Four kinds of moment
Plant water logging group and control group respectively take 22 plants, wherein 12 plants are long for measuring the plant height in ecological index, total number of sheets and total leaf, 10
Strain is for measuring its root activity；Its biomass is measured after having measured again, the stem for randomly selecting survival measures its mda content；
Then the root of drying is taken out, crushes, measures the content of its total Soluble Sugar and starch；
It (3) will be by winter postflood Bermuda grass progress restoration ecosystem experiment for a long time
The Bermuda grass cultivated is subjected to the complete waterlogging of different time, respectively water logging 0d, 30d, 60d, 90d, 120d, 150d
And 180d, it is each to take out 10 plants for restoration ecosystem after water logging；
Before restoration ecosystem starts when 10d, cut off the withered and yellow old stem of plant above ground, on March 22nd, 2012, to water logging 0d, 30d,
Bermuda grass plant after 60d, 90d, 120d and 150d, it is each to take out 10 plants for restoration ecosystem, April 10, to water logging 180d's
Plant carries out restoration ecosystem, in restoration ecosystem 60d, have around old stem seedling turn green or old stem around there is seedling to grow, that is, think
Plant underground brood body survival, it is long in 0d and 6d the measurement total branch of plant of restoration ecosystem experiment beginning, measure plant after 60d
Biomass；The root of drying is taken out, crushes, measures the content of its total Soluble Sugar and starch；
(4) growth rhythm research
Started growth rhythm research to Bermuda grass has been survived May 11, experiment start after 1d, 4d, 7d, 10d,
13d, 18d, 23d, 28d, 33d, 40d, 47d, 54d, 61d, 71d, 81d, 91d, 106d, 121d, 141d, 30 plants of marks of random measurement
Total branch length, the length of the 3rd, 4 young leaves of total number of sheets and top in the ecological index for the Bermuda grass plant remembered, and it is each random
Acquire 10 plants of measurement biomass, the root of drying and stem separated and crushed, measure its total Soluble Sugar and content of starch and root,
The content of nitrogen and phosphorous of stem, leaf；
The measuring method of the chlorophyll is：Plant healthy leaves are acquired, both ends of the blade is removed, removes vein broad in the middle, are used
The content of acetone-ethanol mixed liquor method measurement chlorophyll；
The measuring method of the total Soluble Sugar is：The root or stem for weighing crushing, add 80% dehydrated alcohol of 3ml, and boiling water bath mentions
30min is taken, centrifuging and taking supernatant extracts 2 times repeatedly, is settled to 10ml, the measurement for total Soluble Sugar；
The measuring method of the starch is：Add 4.6mol/L perchloric acid with the residue of measurement total Soluble Sugar extracting solution filtering
2ml, boiling water bath 15min add pure water to 10ml, and centrifugation takes supernatant, so extracts again 1 time, with pure water residue 2 times, from
The heart takes supernatant, and supernatant is merged, and is settled to 50ml, the measurement for starch；
The measuring method of the root activity is：Root activity is measured with TTC method；
The measuring method of the malonaldehyde is：The stem for taking 0.5g Bermuda grass to survive at random, adds 10% trichloroacetic acid of 5ml, grinds
Mill, centrifugation, isolates supernatant；2ml supernatant is taken, 0.5% thiobarbituricacidα- of 2ml is added to mix, boiling water bath 20min is cold
But, it is centrifuged, measures its OD value；
The measuring method indophenol blue colorimetry of the nitrogen content；
The method for measuring phosphor content molybdenum antimony resistance colorimetric method.
2. the method according to claim 1 repaired using Bermuda grass to reservoir drawdown band, which is characterized in that described
Investigation is carried out to the plant of reservoir drawdown band and screening refers to investigation Lake Baihua falling zone plant species distribution, it is raw to count its species
Type living, counts Lake Baihua reservoir drawdown band vascular plant profile map, show that periodical water logging influences the distribution of plant；It is right
Its different gradient growing plants is counted, and obtaining the first, second and third gradient has Bermuda grass growth, and coverage rate is high.
3. the method according to claim 2 repaired using Bermuda grass to reservoir drawdown band, which is characterized in that described
For vegetation based on Bermuda grass and Hemarthria compressa, the coverage of both plants is 40%~60% in first gradient, biomass difference
For 0.19~0.38kgm-2With 0.08~0.13kgm-2, which there is not other plant；Second gradient whole year water logging
Time is 4~6 months, there is the fluctuation of apparent periodic water level, is also the covering based on Bermuda grass and Hemarthria compressa in the gradient
Degree increases to 85%~100%, and biomass is respectively 0.24~0.40kgm-2With 0.12~0.17kgm-2, and occur
Composite family, polygonaceae species, diversity increased, but the coverage of composite family, polygonaceae and other plant is respectively less than 5%, biomass
Again smaller than 0.05kgm-2；The 3rd gradient water logging time is shorter, and the coverage of Hemarthria compressa and Bermuda grass is 100% in the gradient,
Biomass also increases to 0.26~0.53kgm-2With 0.16~0.22kgm-2。
4. the method according to claim 1 repaired using Bermuda grass to reservoir drawdown band, which is characterized in that described
The measuring method of ecological index include：
Plant height：The height of plant above ground first section to top phyllopodium is surveyed with measuring tool；
Total number of sheets：Manually count the plant above ground whole number of sheets；
Total leaf is long：The length that the green portion of plant whole blade is measured with measuring tool, averages；
Total branch is long：The Branches of Different Orders length summation of plant above ground one section or more is measured with measuring tool；
Biomass：The whole strain of plant is cleaned, it is heavy to weigh its with counter balance in 80 DEG C of baking 3d to constant weight by 103 DEG C of water-removing 20min
Amount.
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