CN105027729B - The method of the wet dry Tibetan of Panax notoginseng seeds control - Google Patents
The method of the wet dry Tibetan of Panax notoginseng seeds control Download PDFInfo
- Publication number
- CN105027729B CN105027729B CN201510363280.7A CN201510363280A CN105027729B CN 105027729 B CN105027729 B CN 105027729B CN 201510363280 A CN201510363280 A CN 201510363280A CN 105027729 B CN105027729 B CN 105027729B
- Authority
- CN
- China
- Prior art keywords
- seed
- tibetan
- perlite
- time
- control
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
The invention discloses the method for the wet dry Tibetan of Panax notoginseng seeds control.It comprises the following steps：1) Seed Treatment：The ripening fruits of pseudo-ginseng is gathered, is rubbed with the hands in water and is removed pericarp, obtain the seed of mature and plump, cleaned with clear water after sterilization；2) seed drying：By step 1) seed that obtains dries in the shade；3) by step 2) treatment seed mix with dry perlite, it is divided in open-top receptacle, then open-top receptacle is placed on again and clammy Tibetan is controlled in closed container, wherein closed container bottom adds potassium nitrate solution, open-top receptacle is not contacted potassium nitrate solution with band dummy receptacle；4) by step 3) treatment seed with moisten perlite mix, it is then stored refrigerated.Use of the present invention can control the sealing container of certain environment humidity to the clammy Tibetan of seed control, can extend the morphological development time of embryo；And seed is extended after embryo germination to the time of breaking dormancy, and it is readily transported and stores, be conducive to improving the germination percentage and emergence rate after seed plantation.
Description
Technical field
The present invention relates to a kind of method of the wet dry Tibetan of Panax notoginseng seeds control, belong to Chinese medicine field of storage.
Background technology
Pseudo-ginseng is Araliaceae Panax herbaceos perennial, also known as invaluable, blood ginseng, pseudo-ginseng etc., its root, stem, leaf, flower
Can be used as medicine, be the traditional rare traditional Chinese medicine of China, warm in nature, sweet and slightly bitter taste the effect of with hemostasia and dissipation blood stasis, swelling and pain relieving, is
The primary raw material of the Chinese patent drug such as " Yunnan Baiyao ", " Fufang Danshen Pian ", " compound danshen dripping pills ".Modern pharmacological research shows, three
Seven contain 51 kinds of arasaponins, 77 kinds of volatile oil with beta-elemene as representative, 17 kinds of amino acid with dencichine as representative with
And the various active material such as notoginseng polysaccharide, pseudo-ginseng flavones, in hematological system, cardiovascular system, nervous system, immune system, generation
Thanking system and the aspect such as anti-inflammatory, anti-aging, antitumor all has pharmacological activity and unique curative effect.Pseudo-ginseng medicinal function is extensive,
Significantly, bioavilability is high, has huge exploitation in fields such as medicine, health products, cosmetics, functional food for pharmacology, drug effect
Potentiality and wide market prospects.In recent years, being significantly increased and artificial excessively excavation with market demand, wild pseudo-ginseng is
It is endangered, presently mainly artificial cultivation.
Easily inactivated after the seed dehydration of Desiccation Sensitivity, so as to lose sprouting ability, pole not storage tolerance.Research shows,
Pseudo-ginseng belongs to Desiccation Sensitivity seed, and the water content of Panax notoginseng seeds is 70% or so during natural maturity, at room temperature (20 DEG C, HR
=30%) dry in the shade 1 day, water content will drop to 40% or so, and germination rate drops to 50% or so；Dry in the shade 2 days, under water content
20% or so is dropped to, germination rate is only 10%.According to the literature under field conditions (factors) (winter of warm and moist), pseudo-ginseng producing region
The life-span of Panax notoginseng seeds is only 15 days or so.In production, Panax notoginseng seeds can only be with adopting with broadcasting, it is impossible to storage long, can not be grown
Distance transport, seriously hinders the operation of Panax notoginseng seeds and the development of introducing and planting.Additionally, pseudo-ginseng producing region also meets with often in recent years
The natural calamities such as snow disaster are met, due to lacking suitable the storage of seeds method, cannot be reseeded in time in production, cause huge
Economic loss, therefore, Seed storage turns into the limiting factor for becoming increasingly conspicuous in cultivation of pseudo-ginseng and production process.
The mature seed that Panax notoginseng seeds also have morphological dormacy, new harvesting has spheroidal embryo, not yet develops complete, in normal temperature
Lower sand storage, by after embryo completion morphology development in 45~60 days, seed just starts to sprout.If because the seed of new harvesting enters not in time
Row effectively treatment is sowed immediately, and seed vitality is just easily lost, so only in sand storage pseudo-ginseng method under normal temperature in production practices,
Seed vitality is set to extend 60~90 days, but the method can not prevent embryo morphological development process.Therefore, it is anxious in cultivation of pseudo-ginseng production
A kind of method for extending the Seed storage time is needed, seed vitality is maintained with long-time, it is ensured that percentage of seedgermination, improved pseudo-ginseng and emerge
Rate, while reseeding offer condition during for other regional introducing and plantings and natural calamity.
The content of the invention
It is an object of the invention to provide a kind of method of the wet dry Tibetan of Panax notoginseng seeds control, the inventive method can extend the shape of embryo
State development time；And seed is extended after embryo germination to the time of breaking dormancy, during with the storage for significantly extending seed
Between and break the dormancy of seed, be readily transported and store, be conducive to improving the germination percentage and emergence rate after seed plantation.
The method of the wet dry Tibetan of Panax notoginseng seeds control that the present invention is provided, comprises the following steps：1) Seed Treatment：Collection pseudo-ginseng
Ripening fruits, rubs with the hands in water and removes pericarp, obtains the seed of mature and plump, is cleaned with clear water after sterilization；
2) seed drying：By step 1) seed that obtains dries in the shade；
3) by step 2) treatment seed mix with dry perlite, be divided in open-top receptacle, then again will described in
Open-top receptacle controls clammy Tibetan in being placed on closed container, wherein the closed container bottom adds potassium nitrate solution, with band it is empty every
Plate makes the open-top receptacle not contact the potassium nitrate solution；
4) by step 3) treatment seed with moisten perlite mix, it is then stored refrigerated.
Heretofore described ripening fruits is healthy mature fruit；
Above-mentioned method, step 1) in, the reagent of the sterilization is liquor natrii hypochloritis；
The reagent concentration of the sterilization can be 0.1%~2.5%, concretely 0.1%；
The time of the sterilization can be 5~10min, concretely 10min；
The purpose of the sterilization is to kill the microorganism of the surface of the seed, prevents the seed in follow-up storage
Gone mouldy in journey；Cleaned with clear water again after the sterilization, in case the reagent of the sterilization is remained on the seed,
The seed vitality is caused to damage.
Above-mentioned method, the water content of the seed that dries in the shade can be 50~58%, concretely 55%.
In the present invention, described drying in the shade refers to be placed on to avoid direct sunlight, shady and cool place；
The condition dried in the shade is：Temperature can be 10~30 DEG C, and concretely 20 DEG C, relative humidity can be 20~70%,
Concretely 40%；
The dry time can be 24~72h, concretely 24h.
Above-mentioned method, step 3) in, the seed can be 1 with the volume ratio of the dry perlite：0.5~2, tool
Body can be 1：1；
The temperature of the clammy Tibetan of the control can be 2~4 DEG C, concretely 4 DEG C, time of the clammy Tibetan of control can for 180~
240d, relative humidity in the closed container can be 90~95%, concretely 93%, seed is placed on open-top receptacle
In, then be placed in closed container of the bottom equipped with potassium nitrate solution, to keep neutron absorption moisture, make the work of holding seed
Power.
Above-mentioned method, the potassium nitrate solution is saturation potassium nitrate solution；
Step 3) also include that the open-top receptacle sterilizes and dries and/or carried out at least 1 time during the clammy Tibetan of control
The step of breathing freely and stir, heretofore described open-top receptacle can use tissue culture bottle, glass open-top receptacle or plastics open-top receptacle.
Above-mentioned method, it is described ventilative and stir that to carry out number of times can be 1~9 time in step 3, concretely 8 times, every one
The section time breathes freely and stirs, and seed is obtained enough oxygen, with the vigor of sustaining life；
It can be 14~30d to carry out time interval that is described ventilative and stirring, concretely 30d.
Above-mentioned method, the seed of the refrigeration can be 1 with the volume ratio of the moistening perlite：3~10, concretely
1:10。
Above-mentioned method, the water content of the moistening perlite can be 20~80%, concretely 80%, what moisture content referred to
It is to be calculated according to weight/mass percentage composition.
Above-mentioned method, step 4) in, the temperature of the refrigeration can be 2~4 DEG C, concretely 4 DEG C, the refrigeration when
Between can be 60~90d, concretely 60d；
Step 3) be additionally included in the refrigeration during carry out at least 1 time it is ventilative and the step of stir.
Above-mentioned method, step 3) in, it is described ventilative and stir that to carry out number of times can be 3~12 times, concretely 8 times；
It can be 7~30d to carry out time interval that is described ventilative and stirring, and concretely 7d, ventilative and anti-every a period of time
It is dynamic, seed is obtained enough oxygen, with the vigor of sustaining life.
In the present invention, in step 3) storage in seed be in the wet cold storage environment of control, maintain the stage of morphological dormacy, i.e.,
Embryo there is no development growth or slowly grows, and extend the development time of embryo；In step 4) in seed start
Moisture is absorbed, embryo starts to absorb moisture growth, and seed breach after refrigeration 10 days after breach substantially just can rudiment.In step
The seed of rapid storage stage 3) is reseeded in fully meeting production, the requirement of transport.
The present invention has advantages below：
1st, use can control the sealing container of certain environment humidity to the clammy Tibetan of seed control in the present invention, can extend seed
The embryo development time, and keep seed to sustain life required moisture, it is beneficial to the preservation of seed and the transport of seed.
2nd, the holding time of seed is extended：The method of the present invention can significantly extend the storage time of seed, when making storage
Between by 15 days or so under natural conditions, at most extend to 300 days, pseudo-ginseng can be improved and survived the winter cultivation mode of emerging, be easy to big model
The introducing and planting for enclosing, while offer high quality seed can be reseeded in time when pseudo-ginseng producing region meets with natural calamity.
3rd, moistening perlite can preferably meet the water demand of seed kind embryonic development, regularly stir ventilative, protect seed
Hold vitality.
4th, the seed of present invention storage, breaking dormancy phase, the germination rate of seed is high, plants later emergence rate high.
4th, it is simple and easy to apply：Equipment is simple needed for of the invention, and whole process is concisely convenient, and processing cost is low, is easy in production
In promote the use of.
Specific embodiment
Experimental technique used in following embodiments is conventional method unless otherwise specified.
Material used, reagent etc. in following embodiments, unless otherwise specified, commercially obtain.
The method of the storage of embodiment 1, Panax notoginseng seeds
1) acquisition of seed：3 years raw healthy plants of collection, when fruit maturation, are harvested in batches, and even bennet is together plucked
Under, floral disc and immature fruit are removed, ripening fruits is washed by rubbing with the hands in clear water, and removal exocarp obtains seed, after being cleaned with clear water,
Then with 0.1% the water-soluble liquid disinfectant 10min of sodium hypochlorite (NaClO), then cleaned repeatedly with clear water.
2) seed drying：Put 20 DEG C, humidity be to dry in the shade 24h under the conditions of 40%, obtain the seed that water content is 55%.
3) the wet storage of low temperature control：By step 2) seed and the dry perlite for the treatment of with volume ratio be 1：1 mixing, by it
It is fitted into tissue culture bottle (as open-top receptacle), then tissue culture bottle is put into closed container of the bottom equipped with saturation potassium nitrate solution,
Closed container bottom makes open-top receptacle not contact the saturation potassium nitrate solution of bottom, relative humidity in closed container with dummy receptacle
It is 93%.Then by it is above-mentioned fill seed-bearing closed container and be put into wet stored refrigerated, the stored refrigerated time is controlled in 4 DEG C of refrigerator
It is 240 days, before 240 days store, constantly detects the germination percentage of seed, data is as shown in table 1；Stored refrigerated period every
Breathe freely and stir seed within 1 month, 8 times are carried out altogether and is breathed freely and is stirred seed, to maintain seed appropriate aqueous amount and enough oxygen,
Sustain life power.
4) take out in step 3) the middle wet seed for preserving 240 days of control, use quality percentage moisture content is 80% perlite,
It is 10 according to perlite and seed volume ratio：1 is mixed, and being positioned over 4 DEG C of refrigerators after being dispensed with bundle aperture valve bag is stored up
Deposit, store 60 days；Breathed freely week about during storage and stir seed, 8 times carried out altogether and is breathed freely and is stirred seed, to maintain seed
Appropriate aqueous amount and enough oxygen, sustain life power.
Through the method for above-mentioned storage, storage 300 days afterwards, there are about 85% seed and occur splitting mouth to show money or valuables one carries unintentionally, it was demonstrated that present invention side
Method can extend Seed storage time and breaking dormancy.
Seed after being preserved through the inventive method carries out germination percentage test：Seed after preserving 300 days is placed in equipped with moistening
In the culture dish of perlite, the quality percentage moisture content for moistening perlite is 90%, using 4 culture dishes, in each culture dish
25 seeds are put, sprouting at 20 DEG C of preference temperature is placed in, germination percentage is higher than 81.33%.Meanwhile, after being preserved through the inventive method
Seed carries out greenhouse pot culture simulated experiment, to detect emergence rate：Seed after preserving 300 days is planted in soil-vermiculite matrix
Culture, using 4 sprouting dishes, sows 25 seeds in each disk, 1 centimetre of substrate water content of depth of planting is 40%, emergence rate
Higher than 76%, as shown in table 1.It is each storage time germination percentage and emergence rate to be entered during seed storage of the present invention in table 1
The result of row detection.As shown in Table 1, the method for the storage and breaking dormancy of Panax notoginseng seeds of the present invention, can significantly extend seed
Storage time, makes seed keep vitality higher, and the germination percentage and emergence rate after plantation are high.
The stored seedses time of table 1 and vigour changes
Comparative example,
It is identical with method in the embodiment of the present invention 1；Unlike：
Step 3) in stored refrigerated time be 270, wherein stored refrigerated period is ventilative every 1 month and stirs seed,
9 seeds of breathing freely and stir are carried out altogether；
Step 4) in time of stored under refrigeration be 90 days, wherein storage period week about breathe freely and stir seed by the time,
12 seeds of breathing freely and stir are carried out altogether.
The total storage time of method is the seed after 360 days in example by contrast, and the seed of breaking dormancy splits mouth dew after storage
In vain, according to germination percentage test is carried out in the embodiment of the present invention 1, germination percentage is 0；Simultaneously by seed according in the embodiment of the present invention 1
Detection of emerging is carried out, emergence rate is 0, as shown in table 1.
Claims (8)
1. a kind of method of the wet dry Tibetan of Panax notoginseng seeds control, comprises the following steps：1) Seed Treatment：The ripening fruits of pseudo-ginseng is gathered,
Rubbed with the hands in water and remove pericarp, obtain the seed of mature and plump, cleaned with clear water after sterilization；
The reagent of the sterilization is liquor natrii hypochloritis；The reagent concentration of the sterilization is 0.1%~2.5%；The sterilization
Time is 5~10min；
2) seed drying：By step 1) seed that obtains dries in the shade；
The quality percentage moisture content of the seed that dries in the shade is 50~58%；
3) by step 2) treatment seed mix with dry perlite, be divided in open-top receptacle, then again by the opening
Container controls clammy Tibetan in being placed on closed container, wherein the closed container bottom adds potassium nitrate solution, is made with band dummy receptacle
The open-top receptacle does not contact the potassium nitrate solution；
4) by step 3) treatment seed with moisten perlite mix, it is then stored refrigerated.
2. method according to claim 1, it is characterised in that：Step 3) in, the seed and the dry perlite
Volume ratio be 1：0.5~2；
The temperature of the clammy Tibetan of control is 2~4 DEG C, and the time of the clammy Tibetan of control is 180~240d, in the closed container
Relative humidity is 90~95%.
3. method according to claim 1 and 2, it is characterised in that：The potassium nitrate solution is saturation potassium nitrate solution；
Step 3) also include open-top receptacle sterilizing and dry and/or carry out at least 1 time during the clammy Tibetan of control to breathe freely
And the step of stir.
4. method according to claim 3, it is characterised in that：Step 3) in, it is described ventilative and stir carry out number of times for 1~
9 times；
It is 14~30d to carry out time interval that is described ventilative and stirring.
5. method according to claim 1 and 2, it is characterised in that：Step 4) in, the step 3) treatment seed and institute
The volume ratio for stating the perlite of moistening is 1：3~10.
6. method according to claim 1 and 2, it is characterised in that：The water content of the moistening perlite is 20~80%.
7. method according to claim 1 and 2, it is characterised in that：Step 4) in, the temperature of the refrigeration is 2~4 DEG C,
The time of the refrigeration is 60~90d；
Step 3) be additionally included in the refrigeration during carry out at least 1 time it is ventilative and the step of stir.
8. method according to claim 7, it is characterised in that：Step 3) in, it is described ventilative and stir carry out number of times for 3~
12 times；
It is 7~30d to carry out time interval that is described ventilative and stirring.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510363280.7A CN105027729B (en) | 2015-06-26 | 2015-06-26 | The method of the wet dry Tibetan of Panax notoginseng seeds control |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510363280.7A CN105027729B (en) | 2015-06-26 | 2015-06-26 | The method of the wet dry Tibetan of Panax notoginseng seeds control |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105027729A CN105027729A (en) | 2015-11-11 |
| CN105027729B true CN105027729B (en) | 2017-05-31 |
Family
ID=54435226
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510363280.7A Active CN105027729B (en) | 2015-06-26 | 2015-06-26 | The method of the wet dry Tibetan of Panax notoginseng seeds control |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105027729B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109287189B (en) * | 2018-09-11 | 2021-03-09 | 中国农业大学 | Method for storing pseudo-ginseng seeds in trans-year manner and breaking dormancy |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102487628A (en) * | 2011-12-13 | 2012-06-13 | 中国科学院生态环境研究中心 | Method for long-term storage and dormancy breaking for seeds of panax notoginseng |
| CN104521374A (en) * | 2013-07-25 | 2015-04-22 | 文山苗乡三七科技有限公司 | Storage method of pseudo-ginseng seeds |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101123199B1 (en) * | 2008-06-27 | 2012-06-27 | 대한민국 | methods for control of flowering, shortening the growing period of ginseng plantspanax ginseng C.A.Meyer |
-
2015
- 2015-06-26 CN CN201510363280.7A patent/CN105027729B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102487628A (en) * | 2011-12-13 | 2012-06-13 | 中国科学院生态环境研究中心 | Method for long-term storage and dormancy breaking for seeds of panax notoginseng |
| CN104521374A (en) * | 2013-07-25 | 2015-04-22 | 文山苗乡三七科技有限公司 | Storage method of pseudo-ginseng seeds |
Non-Patent Citations (1)
| Title |
|---|
| 三七种子的脱水敏感性与膜质过氧化作用;段银妹 等;《种子》;20140430;第33卷(第4期);第6-9页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN105027729A (en) | 2015-11-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104429965B (en) | The method that high eyebrow Herba Anoectochili roxburghii seed is bred without hormone quickly tissue culture | |
| CN102487628A (en) | Method for long-term storage and dormancy breaking for seeds of panax notoginseng | |
| CN105850725A (en) | Pitaya pollination method | |
| CN104996103B (en) | Pseudo-ginseng seed storage method | |
| CN106852366B (en) | Storage and preservation method of green Chinese onions | |
| CN106717266A (en) | The long-term preservation method of dendrobium candidum capsule/seed | |
| CN109197458A (en) | A kind of breeding method of rhizoma polygonati seedling | |
| CN102771223A (en) | Piptanthus nepalensis seed preservation and seedling raising methods | |
| CN101112147B (en) | Safe hoarding method for Chinese bandoline wood seeds | |
| CN104956860B (en) | A kind of method of the wet dry Tibetan of Panax notoginseng seeds control | |
| CN103597928A (en) | Dormancy after-ripening treatment method for seeds of bulbus fritillariae cirrhosae | |
| CN102498783B (en) | Wild cryptotaenia japonica seed dormancy breaking method | |
| CN101185459B (en) | Normal temperature preserving agent for lingwu date and fresh-keeping method thereof | |
| CN103444309A (en) | Method for quickly germinating paris polyphylla var.chinensis seeds through combination of gibberellin and filter paper | |
| CN109379921A (en) | A method of accelerating rhizoma polygonati seed and sprouts | |
| CN105027729B (en) | The method of the wet dry Tibetan of Panax notoginseng seeds control | |
| CN108668557A (en) | It is a kind of to utilize method of germination corn enrichment lutein and products thereof and application | |
| CN110150120A (en) | Alpha-aminobutyric acid content and the production method of yield is improved in a kind of enrichment beans bean sprout | |
| CN105594844A (en) | Mangosteen preservation method | |
| CN204762296U (en) | A isolated plant that is used for wet storage of pseudo -ginseng seed accuse | |
| CN114451240B (en) | Method for promoting emergence of polygonatum odoratum seeds | |
| CN109526298A (en) | A kind of passion fruit method for treating seeds and its seed propagation method | |
| CN103141181A (en) | Method for promoting rosa chinensis seed to sprout rapidly | |
| CN107155445B (en) | Method for improving endosperm activity of gynura divaricata seeds after low-temperature storage | |
| CN105494612A (en) | Method for inhibiting stems of stored grapes from browning and fruit particles from falling off |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |