CN105011174A - Human body oxidation preventing and additive free compound protein powder - Google Patents
Human body oxidation preventing and additive free compound protein powder Download PDFInfo
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- 239000000843 powder Substances 0.000 title claims abstract description 82
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- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 50
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- 230000003647 oxidation Effects 0.000 title abstract description 11
- 238000007254 oxidation reaction Methods 0.000 title abstract description 11
- 239000000654 additive Substances 0.000 title abstract 2
- 230000000996 additive effect Effects 0.000 title abstract 2
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- 235000017060 Arachis glabrata Nutrition 0.000 claims abstract description 32
- 244000105624 Arachis hypogaea Species 0.000 claims abstract description 32
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- 240000000851 Vaccinium corymbosum Species 0.000 claims abstract description 19
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- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 claims abstract description 18
- 235000019165 vitamin E Nutrition 0.000 claims abstract description 18
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- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 7
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- MVORZMQFXBLMHM-QWRGUYRKSA-N Gly-His-Lys Chemical compound NCCCC[C@@H](C(O)=O)NC(=O)[C@@H](NC(=O)CN)CC1=CN=CN1 MVORZMQFXBLMHM-QWRGUYRKSA-N 0.000 claims description 26
- 108010038983 glycyl-histidyl-lysine Proteins 0.000 claims description 26
- 230000003064 anti-oxidating effect Effects 0.000 claims description 20
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- 235000016709 nutrition Nutrition 0.000 abstract description 5
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- 244000247812 Amorphophallus rivieri Species 0.000 abstract 1
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- 108010038807 Oligopeptides Proteins 0.000 abstract 1
- 102000015636 Oligopeptides Human genes 0.000 abstract 1
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- 235000018102 proteins Nutrition 0.000 description 33
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 20
- 230000003078 antioxidant effect Effects 0.000 description 16
- 239000003963 antioxidant agent Substances 0.000 description 12
- 235000006708 antioxidants Nutrition 0.000 description 12
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 10
- 229930003268 Vitamin C Natural products 0.000 description 10
- 235000019154 vitamin C Nutrition 0.000 description 10
- 239000011718 vitamin C Substances 0.000 description 10
- -1 oxygen radical Chemical class 0.000 description 9
- 150000003254 radicals Chemical class 0.000 description 9
- 239000000243 solution Substances 0.000 description 9
- HHEAADYXPMHMCT-UHFFFAOYSA-N dpph Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1[N]N(C=1C=CC=CC=1)C1=CC=CC=C1 HHEAADYXPMHMCT-UHFFFAOYSA-N 0.000 description 8
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- 239000000463 material Substances 0.000 description 4
- 229910052760 oxygen Inorganic materials 0.000 description 4
- 239000001301 oxygen Substances 0.000 description 4
- WQGWDDDVZFFDIG-UHFFFAOYSA-N pyrogallol Chemical compound OC1=CC=CC(O)=C1O WQGWDDDVZFFDIG-UHFFFAOYSA-N 0.000 description 4
- 230000002000 scavenging effect Effects 0.000 description 4
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- 238000000034 method Methods 0.000 description 3
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- 239000000523 sample Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- OTEKOJQFKOIXMU-UHFFFAOYSA-N 1,4-bis(trichloromethyl)benzene Chemical compound ClC(Cl)(Cl)C1=CC=C(C(Cl)(Cl)Cl)C=C1 OTEKOJQFKOIXMU-UHFFFAOYSA-N 0.000 description 2
- OUUQCZGPVNCOIJ-UHFFFAOYSA-M Superoxide Chemical compound [O-][O] OUUQCZGPVNCOIJ-UHFFFAOYSA-M 0.000 description 2
- 230000032683 aging Effects 0.000 description 2
- 229930014669 anthocyanidin Natural products 0.000 description 2
- 150000001452 anthocyanidin derivatives Chemical class 0.000 description 2
- 235000008758 anthocyanidins Nutrition 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 235000019658 bitter taste Nutrition 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 235000005911 diet Nutrition 0.000 description 2
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- 229940079877 pyrogallol Drugs 0.000 description 2
- 230000008719 thickening Effects 0.000 description 2
- 210000001541 thymus gland Anatomy 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 108010064851 Plant Proteins Proteins 0.000 description 1
- 235000021120 animal protein Nutrition 0.000 description 1
- 230000002605 anti-dotal effect Effects 0.000 description 1
- 230000003026 anti-oxygenic effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
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- 231100000734 genotoxic potential Toxicity 0.000 description 1
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- 230000036541 health Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 208000030159 metabolic disease Diseases 0.000 description 1
- 239000012452 mother liquor Substances 0.000 description 1
- 235000008935 nutritious Nutrition 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 231100000915 pathological change Toxicity 0.000 description 1
- 230000036285 pathological change Effects 0.000 description 1
- 238000005453 pelletization Methods 0.000 description 1
- 235000021118 plant-derived protein Nutrition 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The present invention relates to a human body oxidation preventing and additive free compound protein powder which comprises the following components in weight percentages: peanut protein powder 50%-70%, peanut peptide powder 10%-20%, marine fish oligopeptide powder 5%-15%, blueberry fruit powder 5%-15%, konjac flour 2%-3%, and vitamin E 0.01%-0.016%. The selected peanut peptide powder, fish collagen powder, blueberry fruit powder and vitamin E are matched with the peanut protein; the matching is reasonable and the dose is scientific, and the combined interaction can help the body prevent oxidation and scavenge free radicals. Besides, the compound protein powder is full in protein nutrition and is easily absorbed by the human body.
Description
Technical field
The present invention relates to a kind of dietetic product, especially a kind of additive-free composite protein powder with human body anti-oxidation function.
Background technology
Active oxygen is continuous in vivo to be produced by enzymatic reaction and non-enzymatic reaction, is constantly eliminated again, maintains the pole low-level gained everything and lose nothing in normal human under the synergy of antioxidase and endogenous antioxidant.But when body is in aging, sick, fatigue state, the balance of interior free yl just may be destroyed, and excessive oxygen radical causes a series of oxidative damage to large biological molecule in body.Metabolic disorder and the pathological change of cell, tissue and even whole body just may be caused or increase the weight of when this damage can not be repaired in time or repair and be made mistakes.Research display, the a lot of serious disease of the mankind and aging are all relevant with the oxidative damage of active oxygen to large biological molecule, to fundamentally treat and prevent this kind of disease just must start with from elimination free radical, by catching or cancellation peroxylradicals, recover the balance of interior free yl, i.e. so-called free radical acology.
What China just had from ancient times " Diet cures more than the doctors " says, although specific food and medicine all have antioxidant effect, medicine often has certain side effect, and increase the removing toxic substances burden of liver, food is then relatively safer, and can eat for a long time.And in existing oxidation resistant food, mostly only comprise single antioxidant content, or utilize the antioxidant adding Prof. Du Yucang to reach oxidation resistant object, cause nutrition comprehensive not, antioxidant effect is not remarkable, add that the antioxidant of Prof. Du Yucang may bring potential risk of toxicity, therefore can not meet the requirement of people to nutrition, health, green anti-oxidation functional food.
Summary of the invention
In order to overcome in existing oxidation resistant product, mostly only comprise single antioxidant content, or utilize the antioxidant adding Prof. Du Yucang to reach oxidation resistant object, cause nutrition comprehensive not, functional effect is not remarkable, and the antioxidant of Prof. Du Yucang may bring the problems such as genotoxic potential risk.The invention provides good, the green and anti-oxidant composite protein powder of safety of a kind of compatibility science, antioxidant effect.
For solving the problem, technical scheme provided by the invention is as follows:
A kind of additive-free compound protein powder for human body anti-oxidation function, be made up of the component of following percentage by weight: the oligomeric Gly-His-Lys 5% ~ 15% of peanut protein powder 40% ~ 80%, peanut Gly-His-Lys 10% ~ 20%, marine fish, blueberry powder 5% ~ 15%, konjaku powder 2% ~ 3%, vitamin E 0.01% ~ 0.016%, the percentage by weight sum of its each component is 100%.
The percentage by weight of above-mentioned compound protein powder component is respectively: the oligomeric Gly-His-Lys 10% of peanut protein powder 61.987%, peanut Gly-His-Lys 15%, marine fish, blueberry powder 10%, konjaku powder 3%, vitamin E 0.013%.
Above-mentioned peanut protein powder is from peanut, and its protein content is more than 55%.
Above-mentioned peanut Gly-His-Lys is from peanut protein hydrolysates, and its protein content >=65%, is less than peptide content >=60% of 1000u.
The oligomeric Gly-His-Lys of above-mentioned marine fish is from marine fish collagen hydrolysate, and its protein content >=90%, is less than peptide content >=85% of 1000u.
Above-mentioned blueberry powder, from blueberry, takes the pure natural blueberry powder that the mode of freeze-drying obtains.
Above-mentioned konjaku powder from konjaku, its glucomannans content >=70%, viscosity >=22000mPas.
Said vitamin E, from food enrichment vitamin E, meets the compound source in GB 14880-2012 and limitation interpolation standard.
Compound basis: compound protein powder of the present invention is protein modules and peanut protein powder compound by the peanut Gly-His-Lys with anti-oxidation efficacy, the oligomeric Gly-His-Lys of marine fish, have chosen blueberry powder, the vitamin E with strong anti-oxidation effect simultaneously and carry out reasonable disposition with it, there is comprehensive oxidation resistant effect, and to have chosen natural konjaku powder be further that auxiliary material reaches thickening, stable effect to make product.Wherein, selected peanut Gly-His-Lys and the oligomeric Gly-His-Lys of marine fish are respectively from peanut protein and collagen hydrolysate, and research shows, food source property polypeptide has antioxidation, to DPPH free radical, OH free radical, O
2 -free radical has stronger scavenging action.And blueberry powder is rich in anthocyanidin, anthocyanidin is the antidotal nutritious supplementary pharmaceutical of pure natural, research proves mankind nowadays and finds the most effective antioxidant, its antioxygenic property exceeds 50 times than vitamin E, 200 times are exceeded than vitamin C, it is 100% to the biological effectiveness of human body, within 20 minutes, just can detect in blood after taking.Selected vitamin E, from food enrichment vitamin E, has antioxidation, body cell can be protected from the murder by poisoning of free radical.Konjaku powder, as auxiliary material wherein, makes product reach thickening, stable effect, imparts this product and good can reconstitute stability and local flavor.Compared with prior art, albumen powder of the present invention is combined by animal and plant protein modules, and compatibility is reasonable, comprehensive nutrition; Be compounded with multiple Antioxidative Factors, reach comprehensive oxidation resistant effect.
Accompanying drawing explanation
Fig. 1 is that compound protein powder and vitamin C are to the comparison of DPPH clearance rate
Fig. 2 is that compound protein powder and vitamin C are to O
2-the comparison of clearance rate
Detailed description of the invention
Below in conjunction with example, the present invention will be further described.
Example 1
For an additive-free compound protein powder for human body anti-oxidation function, the percentage by weight of its each component is respectively: the oligomeric Gly-His-Lys 5% of peanut protein powder 77.99%, peanut Gly-His-Lys 10%, marine fish, blueberry powder 5%, konjaku powder 2%, vitamin E 0.01%.
Example 2
For an additive-free compound protein powder for human body anti-oxidation function, the percentage by weight of its each component is respectively: the oligomeric Gly-His-Lys 10% of peanut protein powder 61.987%, peanut Gly-His-Lys 15%, marine fish, blueberry powder 10%, konjaku powder 3%, vitamin E 0.013%.
Example 3
For an additive-free compound protein powder for human body anti-oxidation function, the percentage by weight of its each component is respectively: the oligomeric Gly-His-Lys 15% of peanut protein powder 45.984%, peanut Gly-His-Lys 20%, marine fish, blueberry powder 15%, konjaku powder 4%, vitamin E 0.016%.
The preparation method of example 1 ~ 3 of the present invention is: take peanut protein powder, peanut Gly-His-Lys, the oligomeric Gly-His-Lys of marine fish, blueberry powder, konjaku powder, vitamin E respectively according to percentage by weight, proceed in 300,000 clean areas, first peanut protein powder, peanut Gly-His-Lys, the oligomeric Gly-His-Lys of marine fish are mixed in proportion, take boiling one-step palletizing technique, 60 DEG C ~ about 70 DEG C fluidized granulating 30min; Again in proportion by blueberry powder, konjaku powder, the dilution of vitamin equal proportion, be placed in three-dimensional mixer mixing 30min together with the raw material of above-mentioned granulation; Finally be placed in pelletizing machine (80 ~ 100 order) whole grain, packaging finished product.
Below by compound protein powder, its significant anti-oxidation function is further illustrated to external radicals scavenging experiment.
1. materials and methods
1.1 materials: compound protein powder, prepared by the preparation method provided according to example of the present invention; Hexichol is for bitter taste free acyl radical (DPPH) purchased from American Sigma company; Absolute ethyl alcohol, purchased from Tianjin Heng Xing chemical reagent Manufacturing Co., Ltd, is analyzed pure; Tri methylol amino methane is purchased from upper sea blue season development in science and technology Co., Ltd; Pyrogallol is purchased from Chengdu Ke Long chemical reagent factory; Vitamin C Shi Yao group.
1.2 experimental technique
1.2.1 to the mensuration of the Scavenging activity of DPPH free radical
Accurately take DPPH sample 4mg, with anhydrous alcohol solution and constant volume in 100mL volumetric flask, obtain the DPPH mother liquor that concentration is 0.04mg/mL, keep in Dark Place in 0 ~ 4 DEG C.Compound protein powder sample is made into concentration be respectively 0.1,0.2,0.4,0.8,1.6, the sample solution of 3.2mg/mL, and be that the DPPH solution 4mL of 0.04mg/mL adds test tube respectively with each 1mL of above-mentioned solution and mixes by concentration, fully shake up, place 30min in darkroom after, using absolute ethyl alcohol as blank, under 517nm, measure absorbance A with ELIASA, and by following formulae discovery clearance rate, its oxidation resistance of the larger expression of clearance rate numerical value is stronger.Separately test using the vitamin C of respective concentration as positive controls.
Clearance rate=[(A
0-A
1)/A
0] × 100%
Wherein: A
0for the absorbance of 4.0mL DPPH solution and 1mL absolute ethyl alcohol; A
1for the absorbance of 4.0mL DPPH solution and solution to be measured.
1.2.2 to the mensuration of superoxide anion (O2-) Scavenging activity
Take a certain amount of compound protein powder be made into mass concentration be 0.1,0.2,0.4,0.8,1.6, the solution of 3.2mg/mL.Under constant temperature 25 DEG C of conditions, measure concentration 50mmol/L, tri methylol amino methane (Tris-HCl) the cushioning liquid 2.8mL of pH 8.2, add the pyrogallol solution 0.1mL of concentration 30mmol/L, rapid mixing, measures 1 absorbance at wavelength 320nm place every 30s, and reaction 4.5min terminates, with absorbance, the time is mapped, try to achieve slope and be mouse thymus cells speed (A
0).Measure concentration 50mmol/L, the Tris-HCl cushioning liquid 2.8mL of pH 8.2, adds albumen powder solution 0.1mL, with the mouse thymus cells speed (A of said method working sample at wavelength 320nm place
1), compare test with VC.
Superoxide anion clearance rate=[(A
0-A
1)/A
0] × 100%
2. result
2.1 edible compound protein powders affect (see figure 1) on hexichol for bitter taste free acyl radical (DPPH) clearance rate
As shown in Figure 1, in 0.4 ~ 3.2mg/mL concentration range, the clearance rate of vitamin C to DPPH free radical remains unchanged substantially, and its clearance rate is about 78.9%.Compound protein powder rises with concentration increase gradually to the clearance rate of DPPH free radical, when concentration reaches 0.2mg/mL, namely the clearance rate of compound protein powder to DPPH free radical reach about 82%, higher than single antioxidant content vitamin C, when concentration reaches more than 1.6mg/mL, it is stabilized in about 93.2% to the clearance rate of DPPH free radical.
2.2 edible compound protein powders are to ultra-oxygen anion free radical (O
2 -) clearance rate affect (see figure 2)
As shown in Figure 2, in 0.8 ~ 3.2mg/mL concentration range, vitamin C is to O
2 -the clearance rate of free radical remains unchanged substantially, and its clearance rate is about 76.2%.Compound protein powder is to O
2 -the clearance rate of free radical rises gradually with concentration increase, and when concentration is at 0.1mg/mL, compound protein powder is to O
2 -namely the clearance rate of free radical reaches about 80.9%, and higher than single antioxidant content vitamin C, when concentration reaches more than 0.8mg/mL, it is to O
2 -the clearance rate of free radical is stabilized in about 91.7%.
Result shows, compound protein powder of the present invention has antioxidation, and its comprehensive oxidation resistance is better than single antioxidant content vitamin C.
Claims (7)
1. the additive-free compound protein powder for human body anti-oxidation function, it is characterized in that: described compound protein powder is made up of the component of following weight percents: the oligomeric Gly-His-Lys 5% ~ 15% of peanut protein powder 40% ~ 80%, peanut Gly-His-Lys 10% ~ 20%, marine fish, blueberry powder 5% ~ 15%, konjaku powder 2% ~ 4%, vitamin E 0.01% ~ 0.016%, the percentage by weight sum of its each component is 100%.
2. a kind of additive-free compound protein powder for human body anti-oxidation function according to right 1, is characterized in that: the percentage by weight of described compound protein powder component is respectively: the oligomeric Gly-His-Lys 10% of peanut protein powder 61.987%, peanut Gly-His-Lys 15%, marine fish, blueberry powder 10%, konjaku powder 3%, vitamin E 0.013%.
3. a kind of additive-free compound protein powder for human body anti-oxidation function according to right 1 or 2, is characterized in that: described peanut Gly-His-Lys is from peanut protein hydrolysates, and its protein content >=65%, is less than peptide content >=60% of 1000u.
4. a kind of additive-free compound protein powder for human body anti-oxidation function according to right 1 or 2, it is characterized in that: the oligomeric Gly-His-Lys of described marine fish is from marine fish collagen hydrolysate, its protein content >=90%, is less than peptide content >=85% of 1000u.
5. a kind of additive-free compound protein powder for human body anti-oxidation function according to right 1 or 2, is characterized in that: described blueberry powder, from blueberry, takes the pure natural blueberry powder that freeze-drying mode obtains.
6. a kind of additive-free compound protein powder for human body anti-oxidation function according to right 1 or 2, is characterized in that: described konjaku powder from konjaku, its glucomannans content >=70%, viscosity >=22000mPas.
7. a kind of additive-free compound protein powder for human body anti-oxidation function according to right 1 or 2, it is characterized in that: described vitamin E, from food enrichment vitamin E, meets the compound source in GB 14880-2012 and limitation interpolation standard.
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CN106937695A (en) * | 2017-03-21 | 2017-07-11 | 舟山昌国食品有限公司 | A kind of processing method of meter of fish protein powder |
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CN105942530A (en) * | 2016-05-06 | 2016-09-21 | 浙江海洋大学 | Squid protein powder |
CN106174517A (en) * | 2016-07-25 | 2016-12-07 | 滨州中裕食品有限公司 | A kind of nutrition reinforced type gluten meal new product |
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CN107048414A (en) * | 2017-03-22 | 2017-08-18 | 邵爱珍 | A kind of composite protein powder rich in peanut bioactive peptides and preparation method thereof |
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