CN104996484B - Chinese prickly ash leaf extract and its application in biological pesticide is prepared - Google Patents
Chinese prickly ash leaf extract and its application in biological pesticide is prepared Download PDFInfo
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- CN104996484B CN104996484B CN201510468125.1A CN201510468125A CN104996484B CN 104996484 B CN104996484 B CN 104996484B CN 201510468125 A CN201510468125 A CN 201510468125A CN 104996484 B CN104996484 B CN 104996484B
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Abstract
Application the present invention relates to Chinese prickly ash leaf extract and its in biological pesticide is prepared, the Chinese prickly ash leaf extract are the acetic acid ethyl ester extract of bunge pricklyash leaf Aqueous extracts, are prepared as follows forming：Picking pericarpium zanthoxyli leaf dries in the shade, and adds water to cook extraction 15 times, collecting decoction, decocting liquid heating is concentrated into 5% the 50% of original volume, ethyl acetate extraction is then added, the heated removing ethyl acetate of organic phase of extraction, dries, crush, obtain Chinese prickly ash leaf extract.The present invention gathers bunge pricklyash leaf as raw material after terminating using pepper picking, turn waste into wealth, preparation cost is low, brings huge Social benefit and economic benefit.
Description
Technical field
The invention belongs to active components of plants extractive technique field, and in particular to a kind of Chinese prickly ash leaf extract and its prepare
Application in biological pesticide.
Background technology
Zanthoxylum Rutaceae dungarunga or shrub plant, it is distributed in Central-South, southwestern and Liaoning, Hebei, Shaanxi, Gansu, mountain
The ground such as east, Jiangsu, Anhui, Zhejiang, Jiangxi, Tibet.Feng County Z-bungeanum Maxim development scale reaches 44,000,000 plants, has built up 17
Thousand mu of Chinese prickly ash Demonstration Gardens, 2 ten thousand mu of Chinese prickly ash Demonstration Gardens.2009 annual productions are up to 3500 tons, 1.4 hundred million yuan of the output value, farmers' per capita's Chinese prickly ash
This income is up to 1866 yuan.Chinese prickly ash turns into Feng County agricultural leading industries, is the main source of farmers' income.At present, Chinese prickly ash
Fruit is by people's large-area applications, but the raw material such as bunge pricklyash leaf does not make full use of but.If the composition in bunge pricklyash leaf converted,
Separation, for the added value of further exploitation bunge pricklyash leaf, lengthening manufacturing chain, improving each side such as peasant income has deep meaning
Justice.
The lepidoptera pests such as diamondback moth are the important pests on vegetable crop, their occurrence degree weights, very harmful.In recent years
Do not come, due to single drug and not Scientific Usage of Drugs, already lead to these insects currently used agricultural chemicals is generated it is different degrees of
The a great problem of resistance, referred to as chemical prevention.Meanwhile to control its harm, peasant must not infrequently dispenser, this not only causes agriculture
People's burden, go back the destruction of pollution and natural, ecological of the furniture to environment.Therefore, urgent need is efficient, Environmental compatibility is good, energy
Overcome or delay the pesticide new product of pest resistance to insecticide.
The content of the invention
The present inventor has been surprisingly found that its extract has and kills insect when furtheing investigate the extract activity of bunge pricklyash leaf
Effect.Therefore, it is an object of the invention to provide a kind of botanical biological pesticide, one kind is especially provided using bunge pricklyash leaf as raw material,
A kind of botanical biological pesticide extract obtained by decocting extraction and ethyl acetate extraction.
The object of the present invention is achieved like this：
A kind of Chinese prickly ash leaf extract, described extract are the acetic acid ethyl ester extract of bunge pricklyash leaf Aqueous extracts.Need to illustrate
, bunge pricklyash leaf is rutaceae Chinese prickly ash 1.Zanthoxylum bungeanum Maxim. leaf.
Preferably, Chinese prickly ash leaf extract as described above, acetic acid ethyl ester extract therein are prepared as follows forming：
Picking pericarpium zanthoxyli leaf dries in the shade, and adds water to cook extraction 1-5 times, collecting decoction, decocting liquid is heated to the 5%-50% for being concentrated into original volume, so
Ethyl acetate extraction is added afterwards, the heated removing ethyl acetate of organic phase of extraction, is dried, and is crushed, is obtained Chinese prickly ash leaf extract.
Preferably, Chinese prickly ash leaf extract as described above, decocting liquid therein are concentrated into the 15%-20% of original volume, so
The ethyl acetate for adding equal volume amounts afterwards extracts 2-4 times.
In addition, present invention also offers the industrial new application of Chinese prickly ash leaf extract, i.e., above-mentioned Chinese prickly ash leaves ethyl acetate extraction
Take application of the thing in biological pesticide is prepared；Or above-mentioned bunge pricklyash leaf acetic acid ethyl ester extract is preparing killing Lepidoptera etc.
Application in the biological pesticide of insect；Described lepidoptera pest is preferably diamondback moth.
Compared with prior art, bunge pricklyash leaf extract for preventing and treating insect effect of the invention is more notable, to squamas such as diamondback moths
The control rate of wing mesh insect has reached 100%, simultaneously because botanical pesticide, therefore do not bring any environmental pollution and agriculture
Medicine remains.In addition, pepper picking of the present invention gathers bunge pricklyash leaf as raw material after terminating, turn waste into wealth, preparation cost is low, brings
Huge Social benefit and economic benefit.
Embodiment
It is below the specific embodiment of the present invention, for further illustrating the essentiality content of the present invention, so as to help this
The present invention, the protection domain of but do not limit the invention in any way is more fully understood in art personnel.It is every according to this hair
The simple modifications that bright essentiality content is carried out to technical em- bodiments once belong to the scope of the present invention.
The preparation of the Chinese prickly ash leaf extract of embodiment 1
After ripe pepper picking terminates, picking pericarpium zanthoxyli leaf dries in the shade, and weighs 0.3 kilogram of dry bunge pricklyash leaf, is placed in marmite
Topped up with water is cooked by slow fire 1h, filtering, and decocting liquid is standby；Repetition adds water to cook 2 times, decocts 1h every time, merges the decocting liquid of 3 decoctions.
Decocting liquid heating is concentrated into the 15%-20% of original volume, the ethyl acetate for then adding equal volume amounts extracts 3 times, merges 3 extractions
The organic phase taken, heating remove ethyl acetate, 60 DEG C of drying, crush, obtain 9.4 grams of Chinese prickly ash leaf extract medicinal powder.
The preparation of the Chinese prickly ash leaf extract of embodiment 2
After ripe pepper picking terminates, picking pericarpium zanthoxyli leaf dries in the shade, and weighs 0.3 kilogram of dry bunge pricklyash leaf, is placed in marmite
Topped up with water is cooked by slow fire 1h, filtering, and decocting liquid is standby；Repetition adds water to cook 2 times, decocts 1h every time, merges the decocting liquid of 3 decoctions.
Decocting liquid heating is concentrated into paste, 60 DEG C of drying, crushes, obtains 28.5 grams of Chinese prickly ash leaf extract medicinal powder.
The preparation of the Chinese prickly ash leaf extract of embodiment 3
After ripe pepper picking terminates, picking pericarpium zanthoxyli leaf dries in the shade, and weighs 0.3 kilogram of dry bunge pricklyash leaf, is placed in marmite
Topped up with water is cooked by slow fire 1h, filtering, and decocting liquid is standby；Repetition adds water to cook 2 times, decocts 1h every time, merges the decocting liquid of 3 decoctions.
Decocting liquid heating is concentrated into the 15%-20% of original volume, then the petroleum ether extraction of addition equal volume amounts 3 times, merges 3 extractions
Organic phase, heating remove petroleum ether, 60 DEG C drying, crush, obtain 14.9 grams of Chinese prickly ash leaf extract medicinal powder.
The preparation of the Chinese prickly ash leaf extract of embodiment 4
After ripe pepper picking terminates, picking pericarpium zanthoxyli leaf dries in the shade, and weighs 0.3 kilogram of dry bunge pricklyash leaf, is placed in marmite
Topped up with water is cooked by slow fire 1h, filtering, and decocting liquid is standby；Repetition adds water to cook 2 times, decocts 1h every time, merges the decocting liquid of 3 decoctions.
Decocting liquid heating is concentrated into the 15%-20% of original volume, then the n-hexane extraction of addition equal volume amounts 3 times, merges 3 extractions
Organic phase, heating remove n-hexane, 60 DEG C drying, crush, obtain 8.3 grams of Chinese prickly ash leaf extract medicinal powder.
Controlling experiment of the Chinese prickly ash leaf extract medicinal powder of embodiment 5 to diamondback moth
The Chinese prickly ash leaf extract medicinal powder prepared according to embodiment 1-4 is added in diamondback moth nutrient powder by 0.1 ‰ respectively, so
120 diamondback moth second instar larvaes of every group of access afterwards, it is placed in receptacle and raises (not add medicinal powder as blank control).Raising
Condition is 25-28 DEG C of temperature, humidity 70%-80%, illumination L：D=14h：10h.After raising 48 hours, the age of each group two is observed
The diamondback moth death rate.Judge the dead method of insect：With writing brush point touching larva, it is considered as death if body is motionless.Experiment knot
Fruit is as shown in table 1.
The each group diamondback moth death rate of table 1 compares
Claims (2)
1. a kind of preparation method for the Chinese prickly ash leaf extract for killing lepidoptera pest, it is characterised in that this method includes following step
Suddenly：Picking pericarpium zanthoxyli leaf dries in the shade, and adds water to cook extraction 1-5 times, collecting decoction, decocting liquid is heated to the 5%-50% for being concentrated into original volume,
Then ethyl acetate extraction is added, the heated removing ethyl acetate of organic phase of extraction, is dried, is crushed, obtains bunge pricklyash leaf extraction
Thing.
2. the preparation method of the Chinese prickly ash leaf extract of lepidoptera pest is killed according to claim 1, it is characterised in that described
Decocting liquid be concentrated into the 15%-20% of original volume, the ethyl acetate for then adding equal volume amounts extracts 2-4 times.
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| CN201510468125.1A CN104996484B (en) | 2015-08-03 | 2015-08-03 | Chinese prickly ash leaf extract and its application in biological pesticide is prepared |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510468125.1A CN104996484B (en) | 2015-08-03 | 2015-08-03 | Chinese prickly ash leaf extract and its application in biological pesticide is prepared |
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| CN104996484A CN104996484A (en) | 2015-10-28 |
| CN104996484B true CN104996484B (en) | 2017-11-10 |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105994430A (en) * | 2016-06-08 | 2016-10-12 | 徐伟 | Composition for killing Contarinia sp. |
| CN107853056A (en) * | 2017-12-05 | 2018-03-30 | 深圳市娅安科技有限公司 | A kind of implantation methods of high yield high-quality tealeaves |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1557169A (en) * | 2004-02-02 | 2004-12-29 | 陈华玲 | Xanthoxylin insecticide and its preparation method |
| CN102836260A (en) * | 2012-09-07 | 2012-12-26 | 四川大学 | Method for extracting plant polyphenol with antioxidant activity from wild pepper leaves |
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2015
- 2015-08-03 CN CN201510468125.1A patent/CN104996484B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1557169A (en) * | 2004-02-02 | 2004-12-29 | 陈华玲 | Xanthoxylin insecticide and its preparation method |
| CN102836260A (en) * | 2012-09-07 | 2012-12-26 | 四川大学 | Method for extracting plant polyphenol with antioxidant activity from wild pepper leaves |
Non-Patent Citations (1)
| Title |
|---|
| 超声波辅助提取花椒叶总黄酮及其体外抗氧化性研究;范菁华等;《中国食品学报》;20101231(第6期);第22-27页 * |
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| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB03 | Change of inventor or designer information |
Inventor after: Li Biying Inventor before: Fan Yonggang Inventor before: Jia Hongrui |
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| CB03 | Change of inventor or designer information | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20171012 Address after: 401122 Chongqing City, Yubei District economic and Technological Development Zone by park C40 block C building four layer Applicant after: CHONGQING HONOROAD ANIMAL HEALTH CO., LTD. Address before: 722405 Baoji city of Shaanxi province Fengxian huangniupu Long Beach No. 256 village seven groups Applicant before: Jia Hongrui |
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