CN104988076A - Phellinus igniarius and application thereof in wood crack bioremediation - Google Patents
Phellinus igniarius and application thereof in wood crack bioremediation Download PDFInfo
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- CN104988076A CN104988076A CN201510410109.7A CN201510410109A CN104988076A CN 104988076 A CN104988076 A CN 104988076A CN 201510410109 A CN201510410109 A CN 201510410109A CN 104988076 A CN104988076 A CN 104988076A
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Abstract
The invention discloses phellinus igniarius SX01, which has a Latin scientific name Phellinus igniarius SX01 and is preserved in China Center for Type Culture Collection with a preservation number: CCTCC M 2015355. The invention also relates to a phellinus igniarius culture prepared by utilizing phellinus igniarius and application of the phellinus igniarius culture in wood crack bioremediation. When a wood crack is remedied by applying the phellinus igniarius culture, the crack and a remedying material are organically combined, so that the crack remedying intensity is greatly enhanced, the appearance is attractive, and the remedying effect is good.
Description
Technical field
The present invention relates to a kind of Sanghuang and culture thereof, and the method utilizing Sanghuang culture to repair wood crackle, be specifically related to microorganism field and woodwork manufacture field.
Background technology
Some woodworks are in producing and selling and use procedure, and can there will be crack, particularly high-grade woodenware along with the increase of duration of service, the appearance in crack not only affects attractive in appearance, also can affect it and be worth, and repairing also quite bothers.Traditional wood method for repairing crack first adds toner with putty powder to be transferred to that then color is close is filled into crack, polishes after waiting drying with sand paper, and after polishing flat, japanning is dry.But the defect adopting the method to repair is the crackle after repairing also can leave obvious flaw, the intensity of putty powder is lower, and wood crack and putty powder are not organically combine, time one is long, crack is there will be again, the situation even occur that putty weighting material loosens completely, coming off between crack and putty weighting material.
Summary of the invention
The object of this invention is to provide a kind of Sanghuang and the application in biological restoration wood crackle thereof, by Sanghuang culture, biological restoration is carried out to wood crackle, the defect existed in prior art can be overcome preferably, crack and repair materials are formed organically combine, substantially increase the intensity of mended crack, appearance looks elegant, repair efficiency is good.
For solving the problems of the technologies described above, the technical solution adopted in the present invention is: a kind of Sanghuang SX01, its Latin name Phellinus igniarius, be preserved in China typical culture collection center on June 4th, 2015, deposit number is: CCTCC M 2015355.
The invention also discloses the application of above-mentioned Sanghuang in biological restoration wood crackle.
The invention still further relates to Sanghuang culture prepared by the Sanghuang described in employing, the substratum of this culture is made up of following raw material by weight: peptone 8-10 part, sucrose 15-20 part, wood dust 15-20 part, water 70-110 part.
In preferred scheme, described wood dust is mixed by one or more in mulberry tree, pine tree, willow, birch, robur.
Further, the particle diameter of described wood dust is 80-100 order.
The preparation method of described Sanghuang culture, concrete steps are: first peptone, sucrose and wood dust are mixed, then add water to stir, at 121 DEG C of sterilizing 40-50min, be cooled to less than 30 DEG C, access Sanghuang SX01 mycelia again, cultivate 20-25 days in 24 DEG C-28 DEG C, treat that mycelia is covered with and namely obtain Sanghuang culture.
The invention still further relates to the application of described Sanghuang culture in biological restoration wood crackle.
A kind of wood crackle biological renovation method, comprises the following steps:
1) Sanghuang culture is prepared;
2) surface of wood crackle is carried out disinfection, then spray nutritive medium;
3) Sanghuang culture is filled into wood cracks, and carry out overlay film send out bacterium cultivate, cover with after crackle until mycelia and take off film, finally carry out surface treatment;
The reparation of wood crackle is completed by aforesaid operations.
Further, adopt mass concentration to be that the spirituous solution of 70%-75% sprays during sterilization, after alcohol volatile dry, can nutritive medium be sprayed, can adopt and dry or drying aid mode that hair dryer dries up; Glucose solution or the sucrose solution of described nutritive medium to be mass concentration be 8-10%.
Further, sending out bacterium culture condition is 24 DEG C-28 DEG C cultivation 10-15 days.Film used is sterile culture container sealed membrane, and available scotch tape is fixed on wood.After taking off film, mycelia can form lignifying structure through 10-15 days, finally with traditional method carry out polishing, repair, japanning, the surface treatment such as drying.
Beneficial effect of the present invention is as follows:
1, in the culturing process of Sanghuang culture, mycelia can grow the depths being deep into and being repaired wood crackle, and compact winding mutual to wood fiber, is formed organicly firmly to combine; Can not crack be there is in time and again or subside in mend.
2, Sanghuang is through the cultivation of certain hour, and due to the evaporation of moisture and the consumption of nutrition, mycelia can form firm lignifying structure gradually, enhances the intensity of repairing position, and very firm;
3, by the one in choose reasonable mulberry tree, pine tree, willow, birch, robur or its mixture as one of filling component, the color of the wood that its color of cultivating the rear mend formed can closely will be repaired, without the need to carrying out toning process.
Sanghuang Phellinus igniarius SX01 of the present invention is now preserved in China typical culture collection center (CCTCC), preservation address is: Bayi Road Luo Jia Shan, Wuchang District, Wuhan City, Hubei Province, preservation date is on June 4th, 2015, and deposit number is CCTCC M 2015355.
Embodiment
Further illustrate the present invention below in conjunction with embodiment, but the scope of protection of present invention is not limited to the scope of embodiment statement.
This kind of Sanghuang is separated the Phellinus sporophore grown on live body mulberry tree, adopts the method disclosed in CN201510048790.5 to be separated, because it is macro fungi, can identify from form.
Embodiment 1:
Adopt Sanghuang culture prepared by Sanghuang, raw material is: peptone 8g, sucrose 15g, particle diameter 80 order pine tree wood dust 15g, mixed, then add water to stir, make the water content of mixture reach 65%, 121 DEG C of sterilizing 50min, be cooled to less than 30 DEG C, the inclined-plane mycelia of access Sanghuang SX01, cultivates 25 days in 24 DEG C, treats that mycelia is covered with and be Sanghuang SX01 culture.
Embodiment 2:
Adopt Sanghuang culture prepared by Sanghuang, raw material is: peptone 10g, sucrose 20g, particle diameter 80 order mulberry tree wood dust 20g, mixed, then add water to stir, make the water content of mixture reach 70%, 121 DEG C of sterilizing 50min, be cooled to less than 30 DEG C, the inclined-plane mycelia of access Sanghuang SX01, cultivates 20 days in 28 DEG C, treats that mycelia is covered with and be Sanghuang SX01 culture.
Embodiment 3:
Adopt Sanghuang culture prepared by Sanghuang, raw material is: peptone 9g, sucrose 18g, particle diameter 100 order mulberry tree wood dust 10g, particle diameter 100 order birch wood dust 8g, mix after, add water to stir, the water content of mixture is made to reach 68%, 121 DEG C of sterilizing 40min, are cooled to less than 30 DEG C, the inclined-plane mycelia of access Sanghuang SX01, cultivate 23 days in 25 DEG C, treat that mycelia is covered with and be Sanghuang SX01 culture.
Embodiment 4:
Adopt Sanghuang culture prepared by Sanghuang, raw material is: peptone 8g, sucrose 20g, particle diameter 100 order robur wood dust 18g, mixed, after add water stir, make the water content of mixture reach 70%, 121 DEG C of sterilizing 40min, be cooled to less than 30 DEG C, the inclined-plane mycelia of access Sanghuang SX01, cultivates 25 days in 25 DEG C, treats that mycelia is covered with and be Sanghuang SX01 culture.
Embodiment 5:
A kind of wood crackle biological renovation method, concrete wood raw material is pine, crack width is 0.5cm, during reparation, 75% spirituous solution Direct spraying is first adopted to carry out surface sterilization in crack surfaces, after being evaporated completely Deng alcohol, syrup nutritive medium containing glucose 8% is sprayed at the wood crack surfaces of above-mentioned sterilization, then in wood crackle, fill the Sanghuang SX01 culture prepared in embodiment 1, cover sterile culture container sealed membrane, and be fixed on wood with scotch tape, cover with mycelia in 24 DEG C of cultivations about 15 days culture surfaces and can take film off, mycelia formed lignifying structure through about 10 days again, finally polish by traditional method, finishing, japanning, dry.
Embodiment 6:
A kind of wood crackle biological renovation method, concrete wood raw material is beech, crack width is 1.5cm, during reparation, first adopt mass concentration be 75% spirituous solution Direct spraying carry out surface sterilization in crack surfaces, after being evaporated completely (also can dry up with hair dryer) Deng alcohol, syrup nutritive medium containing glucose 10% is sprayed at the wood crack surfaces of above-mentioned sterilization, then in wood crackle, fill the Sanghuang SX01 culture prepared in embodiment 2, cover sterile culture container sealed membrane, and be fixed on wood with scotch tape, , culture condition is 28 DEG C and cultivates about 15 days, cover with mycelia to culture surface and can take film off, mycelia formed lignifying structure through about 12 days again, finally polish by traditional method, finishing, japanning, dry.
Embodiment 7:
A kind of wood crackle biological renovation method, concrete wood raw material is camphorwood, crack width is 1.2cm, during reparation, 70% spirituous solution Direct spraying is first adopted to carry out surface sterilization in crack surfaces, after being evaporated completely Deng alcohol, the syrup nutritive medium of mix with sucrose containing glucose 10% is sprayed at the wood crack surfaces of above-mentioned sterilization, then in wood crackle, fill the Sanghuang SX01 culture prepared in embodiment 3, cover sterile culture container sealed membrane, and be fixed on wood with scotch tape, cultivate about 12 days in 25 DEG C, cover with mycelia to culture surface and can take film off, mycelia formed lignifying structure through about 13 days again, finally polish by traditional method, finishing, japanning, dry.
Embodiment 8:
A kind of wood crackle biological renovation method, concrete wood raw material is oak, crack width is 0.8cm, during reparation, 72% spirituous solution Direct spraying is first adopted to carry out surface sterilization in crack surfaces, after being evaporated completely Deng alcohol, syrup nutritive medium containing sucrose 8% is sprayed at the wood crack surfaces of above-mentioned sterilization, then in wood crackle, fill the Sanghuang SX01 culture prepared in embodiment 4, cover sterile culture container sealed membrane, and be fixed on wood with scotch tape, culture condition is 25 DEG C and cultivates 14 days, cover with mycelia to culture surface and can take film off, mycelia formed lignifying structure through 15 days again, finally polish by traditional method, finishing, japanning, dry.
Claims (10)
1. a Sanghuang SX01, Latin name Phellinus igniarius SX01, is characterized in that: be preserved in state's Type Tissue Collection on June 4th, 2015, deposit number is: CCTCC M 2015355.
2. the application of Sanghuang according to claim 1 in biological restoration wood crackle.
3. the Sanghuang culture adopting Sanghuang according to claim 1 to prepare, is characterized in that: the substratum of this culture is made up of following raw material by weight: peptone 8-10 part, sucrose 15-20 part, wood dust 15-20 part, water 70-110 part.
4. Sanghuang culture according to claim 3, is characterized in that: described wood dust is mixed by one or more in mulberry tree, pine tree, willow, birch, robur.
5. Sanghuang culture according to claim 3, is characterized in that: the particle diameter of described wood dust is 80-100 order.
6. the preparation method of the Sanghuang culture according to claim 3-5 any one, it is characterized in that, concrete steps are: first peptone, sucrose and wood dust are mixed, then add water to stir, at 121 DEG C of sterilizing 40-50min, be cooled to less than 30 DEG C, then access Sanghuang SX01 mycelia, cultivate 20-25 days in 24 DEG C-28 DEG C, treat that mycelia is covered with and namely obtain Sanghuang culture.
7. according to the application of Sanghuang culture in biological restoration wood crackle that any one in claim 3-6 obtains.
8. a wood crackle biological renovation method, is characterized in that, comprises the following steps:
1) Sanghuang culture is prepared;
2) surface of wood crackle is carried out disinfection, then spray nutritive medium;
3) Sanghuang culture is filled into wood cracks, and carry out overlay film send out bacterium cultivate, cover with after crackle until mycelia and take off film, place 10-15 days, to form lignifying structure, finally carry out surface treatment;
The reparation of wood crackle is completed by aforesaid operations.
9. restorative procedure according to claim 8, is characterized in that: adopt mass concentration to be that the spirituous solution of 70%-75% sprays during sterilization, can spray nutritive medium after alcohol volatile dry; Glucose solution or the sucrose solution of described nutritive medium to be mass concentration be 8-10%.
10. restorative procedure according to claim 8, is characterized in that: sending out bacterium culture condition is 24 DEG C-28 DEG C cultivation 10-15 days.
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| CN201510410109.7A CN104988076B (en) | 2015-07-14 | 2015-07-14 | A kind of Sanghuang and its application in biological prosthetic wood crackle |
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| CN201510410109.7A CN104988076B (en) | 2015-07-14 | 2015-07-14 | A kind of Sanghuang and its application in biological prosthetic wood crackle |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009072687A1 (en) * | 2007-12-07 | 2009-06-11 | Hankook Pharm. Co., Inc. | Composition for inhibition of trasplant rejection containing the phellinus linteus mycellia extract as an active ingredient |
| CN101836595A (en) * | 2009-03-18 | 2010-09-22 | 西南科技大学 | Medicinal mushroom strain and method for culturing fruiting bodies thereof |
| CN104630074A (en) * | 2015-01-30 | 2015-05-20 | 三峡大学 | Phellinus linteus sporocarp hypha separating method |
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2015
- 2015-07-14 CN CN201510410109.7A patent/CN104988076B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009072687A1 (en) * | 2007-12-07 | 2009-06-11 | Hankook Pharm. Co., Inc. | Composition for inhibition of trasplant rejection containing the phellinus linteus mycellia extract as an active ingredient |
| CN101836595A (en) * | 2009-03-18 | 2010-09-22 | 西南科技大学 | Medicinal mushroom strain and method for culturing fruiting bodies thereof |
| CN104630074A (en) * | 2015-01-30 | 2015-05-20 | 三峡大学 | Phellinus linteus sporocarp hypha separating method |
Non-Patent Citations (2)
| Title |
|---|
| 宋力 等: "桑黄的研究进展", 《中国食用菌》 * |
| 陈子繁 等: "论细菌纤维素对木质文物的修复", 《江汉考古》 * |
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