CN104988076B - A kind of Sanghuang and its application in biological prosthetic wood crackle - Google Patents
A kind of Sanghuang and its application in biological prosthetic wood crackle Download PDFInfo
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- CN104988076B CN104988076B CN201510410109.7A CN201510410109A CN104988076B CN 104988076 B CN104988076 B CN 104988076B CN 201510410109 A CN201510410109 A CN 201510410109A CN 104988076 B CN104988076 B CN 104988076B
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Abstract
The invention discloses a kind of Sanghuang SX01, Latin name Phellinus igniarius SX01 to be preserved in China typical culture collection center, deposit number is：CCTCC M 2015355, the invention further relates to the Sanghuang cultures prepared using Sanghuang；And application of the Sanghuang culture in biological prosthetic wood crackle.When repairing wood crack using Sanghuang culture, crack and repair materials can form combination, substantially increase the intensity of mended crack, appearance looks elegant, and repair efficiency is good.
Description
Technical field
The present invention relates to a kind of Sanghuang and its culture and using Sanghuang culture to wood
The method that crackle is repaired, and in particular to microorganism field and woodwork manufacture field.
Background technology
Some woodworks can will appear crack, especially during producing and selling and use with the increase of usage time
It is high-grade woodenware, the appearance in crack not only influences beauty, can also influence its value, and repair also fairly cumbersome.Traditional wood
Head method for repairing crack is that be first transferred to color with powder of lacquer putty for use on additive color powder close and then be filled into crack, wait it is dry after beaten with sand paper
Mill, after polishing flat, japanning drying.But it is that crackle after repairing can also there are apparent using the defects of this method repairing
Flaw, the intensity of powder of lacquer putty for use on is relatively low, and wood crack is not to organically combine with powder of lacquer putty for use on, and for a long time, crack is filled with putty
It will appear crack again between object or even occur the situation that putty filler is loosened, come off completely.
Invention content
A kind of application the object of the present invention is to provide Sanghuang and its in biological prosthetic wood crackle, passes through
Sanghuang culture is biological prosthetic to the progress of wood crackle, can preferably overcome defect in the prior art,
It forms crack and repair materials to organically combine, substantially increases the intensity of mended crack, appearance looks elegant, repair efficiency is good.
In order to solve the above technical problems, the technical solution adopted in the present invention is：A kind of Sanghuang SX01 is drawn
Fourth literature name Phellinus igniarius were preserved in China typical culture collection center, preservation on June 4th, 2015
Number is：CCTCC M 2015355.
The invention also discloses application of the above-mentioned Sanghuang in biological prosthetic wood crackle.
The invention further relates to the Sanghuang culture prepared using the Sanghuang, the culture
Culture medium is made of following raw material by weight：8-10 parts of peptone, 15-20 parts of sucrose, 15-20 parts of wood dust, water 70-
110 parts.
In preferred scheme, the wood dust is by the mixing of one or more of mulberry tree, pine tree, willow, birch, robur
It is made.
Further, the grain size of the wood dust is 80-100 mesh.
The preparation method of the Sanghuang culture, the specific steps are：First by peptone, sucrose and wood dust
It is uniformly mixed, then adds in water and be stirred, sterilize 40-50min at 121 DEG C, is cooled to 30 DEG C hereinafter, accessing fiery fixing fillet layer again
Pore fungi SX01 mycelia is cultivated 20-25 days in 24 DEG C -28 DEG C, treats that mycelia is covered with and obtain Sanghuang culture.
The invention further relates to application of the Sanghuang culture in biological prosthetic wood crackle.
A kind of wood crackle biological renovation method, includes the following steps：
1）Prepare Sanghuang culture；
2）It carries out disinfection to the surface of wood crackle, then sprays nutrient solution；
3）Sanghuang culture is filled into wood cracks, and carries out overlay film bacterium germination culture, treats that mycelia is covered with
Film is taken off after crackle, is finally surface-treated；
The reparation of wood crackle is completed by aforesaid operations.
Further, mass concentration is used to be sprayed for the alcoholic solution of 70%-75% during disinfection, alcohol volatile dry it
Afterwards be sprayable nutrient solution, can be used dry or hair dryer drying auxiliary drying mode；The nutrient solution is dense for quality
Spend the glucose solution or sucrose solution for 8-10%.
Further, bacterium germination condition of culture is cultivated 10-15 days for 24 DEG C -28 DEG C.Film used is sealed for sterile culture container
Membrana oralis can be fixed on wood with adhesive tape.Mycelia can form lignifying structure by 10-15 days after taking off film, finally use
The surface treatments such as traditional method is polished, modified, japanning, drying.
Beneficial effects of the present invention are as follows：
1st, in the incubation of Sanghuang culture, mycelia can grow the depth for being deep into and being repaired wood crackle
Place, and be mutually closely wound with wood fiber, form organic firm combination；Crack will not occur at any time and again in mend
Or it collapses.
2nd, Sanghuang passes through the culture of certain time, and due to the consumption of evaporation and the nutrition of moisture, mycelia can be by
Firm lignifying structure is gradually formed, enhances the intensity for repairing position, and very firm；
3rd, it is used as filling component by reasonably selecting one kind in mulberry tree, pine tree, willow, birch, robur or its mixture
One of so that after it is cultivated color of mend for being formed can the very close wood to be repaired color, without into
Row toning is handled.
The Sanghuang Phellinus igniarius SX01 of the present invention are now preserved in Chinese Typical Representative culture guarantor
Tibetan center (CCTCC), preservation address are：Wuhan City, Hubei Province Wuchang District Bayi Road Luo Jia Shan, preservation date are June 4 in 2015
Day, deposit number is CCTCC M 2015355.
Specific embodiment
It is further illustrated the present invention with reference to embodiment, but the scope of protection of present invention is not limited to implement
The range of example statement.
This kind of Sanghuang is isolated from the Phellinus fructification grown on live body mulberry tree, using CN201510048790.5
Disclosed method can be detached, and since it is macro fungi, can be identified from form.
Embodiment 1：
The Sanghuang culture prepared using Sanghuang, raw material are：Peptone 8g, sucrose 15g, grain size
80 mesh pine tree wood dust 15g, are mixed uniformly, then add in water stirring, the water content of mixture are made to reach 65%, 121 DEG C go out
Bacterium 50min is cooled to 30 DEG C hereinafter, the inclined-plane mycelia of access Sanghuang SX01, cultivates 25 days in 24 DEG C, treat that mycelia is long
Completely it is Sanghuang SX01 cultures.
Embodiment 2：
The Sanghuang culture prepared using Sanghuang, raw material are：Peptone 10g, sucrose 20g, grain
80 mesh mulberry tree wood dust 20g of diameter, is mixed uniformly, then adds in water stirring, the water content of mixture is made to reach 70%, 121 DEG C
Sterilize 50min, is cooled to 30 DEG C hereinafter, the inclined-plane mycelia of access Sanghuang SX01, cultivates 20 days in 28 DEG C, treat mycelia
It covers with as Sanghuang SX01 cultures.
Embodiment 3：
The Sanghuang culture prepared using Sanghuang, raw material are：Peptone 9g, sucrose 18g, grain size
100 mesh mulberry tree wood dust 10g, 100 mesh birch wood dust 8g of grain size, after mixing add in water stirring, make the aqueous of mixture
Amount reaches 68%, and 121 DEG C of sterilizing 40min are cooled to 30 DEG C hereinafter, the inclined-plane mycelia of Sanghuang SX01 is accessed, in 25 DEG C
Culture 23 days, treats that mycelia is covered with as Sanghuang SX01 cultures.
Embodiment 4：
The Sanghuang culture prepared using Sanghuang, raw material are：Peptone 8g, sucrose 20g, grain size
100 mesh robur wood dust 18g are mixed uniformly, rear to add in water stirring, the water content of mixture are made to reach 70%, 121 DEG C go out
Bacterium 40min is cooled to 30 DEG C hereinafter, the inclined-plane mycelia of access Sanghuang SX01, cultivates 25 days in 25 DEG C, treat that mycelia is long
Completely it is Sanghuang SX01 cultures.
Embodiment 5：
A kind of wood crackle biological renovation method, specific wood raw material are pine, crack width 0.5cm, during reparation,
Surface sterilization is first carried out in crack surfaces using 75% alcoholic solution Direct spraying, it, will be containing glucose 8% after alcohol is waited to be evaporated completely
Syrup nutrient solution is sprayed at the above-mentioned wood crack surfaces sterilized, then fills into wood crackle and is prepared in embodiment 1
Sanghuang SX01 cultures, cover sterile culture container sealed membrane, and be fixed on wood with adhesive tape, in 24
DEG C 15 days or so culture surfaces of culture, which cover with mycelia, can take film off, and mycelia formed lignifying structure using 10 days or so,
It finally polished, modified with traditional method, japanning, being dried.
Embodiment 6：
A kind of wood crackle biological renovation method, specific wood raw material are beech, crack width 1.5cm, during reparation,
Mass concentration is first used to carry out surface sterilization in crack surfaces for 75% alcoholic solution Direct spraying, alcohol is waited to be evaporated completely（Also may be used
Electricity consumption blowing drying）Afterwards, the syrup nutrient solution containing glucose 10% is sprayed at the above-mentioned wood crack surfaces sterilized, then
The Sanghuang SX01 cultures prepared in embodiment 2, covering sterile culture container sealing are filled into wood crackle
Film, and be fixed on wood with adhesive tape, condition of culture is cultivated 15 days or so for 28 DEG C, until culture surface covers with mycelia
Can take film off, mycelia formed lignifying structure using 12 days or so, finally polished, modified with traditional method, on
Paint, drying.
Embodiment 7：
A kind of wood crackle biological renovation method, specific wood raw material are camphorwood, crack width 1.2cm, during reparation,
Surface sterilization is first carried out in crack surfaces using 70% alcoholic solution Direct spraying, after alcohol is waited to be evaporated completely, glucose and sugarcane will be contained
10% syrup nutrient solution of sugar mixing is sprayed at the above-mentioned wood crack surfaces sterilized, is then filled into wood crackle
The Sanghuang SX01 cultures prepared in example 3 are applied, cover sterile culture container sealed membrane, and fixed with adhesive tape
In on wood, cultivated 12 days or so in 25 DEG C, until culture surface, which covers with mycelia, can take film off, mycelia was using 13 days or so
Lignifying structure is formed, is finally polished, modified with traditional method, japanning, dried.
Embodiment 8：
A kind of wood crackle biological renovation method, specific wood raw material are oak, crack width 0.8cm, during reparation,
Surface sterilization is first carried out in crack surfaces using 72% alcoholic solution Direct spraying, after alcohol is waited to be evaporated completely, by the sugar containing sucrose 8%
Water nutrition liquid is sprayed at the above-mentioned wood crack surfaces sterilized, then fills what is prepared in embodiment 4 into wood crackle
Sanghuang SX01 cultures cover sterile culture container sealed membrane, and are fixed on wood with adhesive tape, cultivate item
Part is cultivated 14 days for 25 DEG C, until culture surface, which covers with mycelia, can take film off, mycelia formed lignifying structure using 15 days,
It finally polished, modified with traditional method, japanning, being dried.
Claims (7)
1. a kind of wood crackle biological renovation method, which is characterized in that include the following steps：
1）Preserving number is used to prepare Sanghuang culture for the Sanghuang SX01 of CCTCC M 2015355；
2）It carries out disinfection to the surface of wood crackle, then sprays nutrient solution；
3）Sanghuang culture is filled into wood cracks, and carries out overlay film bacterium germination culture, treats that mycelia covers with crackle
After take off film, place 10-15 days, to form lignifying structure, be finally surface-treated；
The reparation of wood crackle is completed by aforesaid operations.
2. restorative procedure according to claim 1, it is characterised in that：Wine of the mass concentration for 70%-75% is used during disinfection
Smart solution is sprayed, and is sprayable nutrient solution after alcohol volatile dry；The nutrient solution is that mass concentration is 8-10%'s
Glucose solution or sucrose solution.
3. restorative procedure according to claim 1, it is characterised in that：Bacterium germination condition of culture cultivates 10-15 for 24 DEG C -28 DEG C
My god.
4. restorative procedure according to claim 1, it is characterised in that：Step 1）In culture use culture medium by pressing
The following raw material of parts by weight meter is made：8-10 parts of peptone, 15-20 parts of sucrose, 15-20 parts of wood dust, 70-110 parts of water.
5. restorative procedure according to claim 4, it is characterised in that：The wood dust is by mulberry tree, pine tree, willow, birch
One or more of tree, robur are mixed.
6. restorative procedure according to claim 5, it is characterised in that：The grain size of the wood dust is 80-100 mesh.
7. restorative procedure according to claim 4, it is characterised in that：The specific preparation method of the culture is：First by egg
White peptone, sucrose and wood dust are uniformly mixed, and are then added in water and are stirred, and sterilize 40-50min at 121 DEG C, be cooled to 30 DEG C with
Under, then the Sanghuang SX01 mycelia that preserving number is CCTCC M 2015355 is accessed, in 24 DEG C of -28 DEG C of culture 20-25
My god, it treats that mycelia is covered with and obtains Sanghuang culture.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009072687A1 (en) * | 2007-12-07 | 2009-06-11 | Hankook Pharm. Co., Inc. | Composition for inhibition of trasplant rejection containing the phellinus linteus mycellia extract as an active ingredient |
| CN101836595A (en) * | 2009-03-18 | 2010-09-22 | 西南科技大学 | Medicinal mushroom strain and method for culturing fruiting bodies thereof |
| CN104630074A (en) * | 2015-01-30 | 2015-05-20 | 三峡大学 | Phellinus linteus sporocarp hypha separating method |
-
2015
- 2015-07-14 CN CN201510410109.7A patent/CN104988076B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009072687A1 (en) * | 2007-12-07 | 2009-06-11 | Hankook Pharm. Co., Inc. | Composition for inhibition of trasplant rejection containing the phellinus linteus mycellia extract as an active ingredient |
| CN101836595A (en) * | 2009-03-18 | 2010-09-22 | 西南科技大学 | Medicinal mushroom strain and method for culturing fruiting bodies thereof |
| CN104630074A (en) * | 2015-01-30 | 2015-05-20 | 三峡大学 | Phellinus linteus sporocarp hypha separating method |
Non-Patent Citations (2)
| Title |
|---|
| 桑黄的研究进展;宋力 等;《中国食用菌》;20050515;第24卷;7-10,15 * |
| 论细菌纤维素对木质文物的修复;陈子繁 等;《江汉考古》;20140615(第s1期);92-95 * |
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