CN102835251A - Submerged fermentation culturing method for medicinal hericium erinaceus mycelium liquid - Google Patents
Submerged fermentation culturing method for medicinal hericium erinaceus mycelium liquid Download PDFInfo
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- CN102835251A CN102835251A CN2012103328447A CN201210332844A CN102835251A CN 102835251 A CN102835251 A CN 102835251A CN 2012103328447 A CN2012103328447 A CN 2012103328447A CN 201210332844 A CN201210332844 A CN 201210332844A CN 102835251 A CN102835251 A CN 102835251A
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Abstract
The invention discloses a submerged fermentation culturing method for a medicinal hericium erinaceus mycelium liquid; compared with a common edible hericium erinaceus, the obtained hericium erinaceus mycelium contains five cancer-fighting substances, seven vitamins, three active cellulases, 18 amino acids, trace elements essential to the human body and on the like except for polysaccharide and polypeptide, wherein contents of cystine, histidine and arginine with larger medicinal values are large; and the 6-7 amino acid identifying points and the protein content (16.65%) of the obtained hericium erinaceus mycelium are respectively 3 times and 1.8 times larger than those of other hericium erinaceus. Infrared spectrum also proves that the hericium erinaceus mycelium also contains a special cancer-fighting organic acid, which is not available in the common hericium erinaceus, except for a hydroxyl ammonia compound. Compared with the traditional manual solid culturing method, the submerged fermentation culturing method disclosed by the invention has the advantages as follows: the breakthrough from manual culture of the hericium erinaceus mycelium to standard and industrial production of the hericium erinaceus mycelium is achieved, the production period is reduced from the 52 days of the traditional culturing method to 14 days, energy is saved and consumption is reduced by 30%, and production cost is reduced by 70%.
Description
Technical field
The present invention relates to microorganism and cultivate technical field, the mycelial cultural method of especially medicinal Hericium erinaceus.
Background technology
Hericium erinaceus; Being to gain the name because its fruit body shape exactly likes the head of little monkey, is a kind of famous and precious edible mushroom edible and medical value that has concurrently, and property is flat, flavor is sweet; Ability sharp the five internal organs, aid digestion; Neurasthenia is treated in nourishing, anticancer, is used to treat the disease and the tumour of digestive systems such as indigestion, stomach ulcer, cancer of the esophagus, cancer of the stomach, duodenal cancer, cardiac cancer.Hericium erinaceus has been successfully applied in the existing product at present, and like commercially available HOUTOU JIANWEI JIAONANG, wherein Hericium erinaceus is the Main Ingredients and Appearance of this product.
The production approach of Hericium erinaceus mainly contains three kinds at present: 1, artificial cultivation, advantage are simple, and cost is low; Shortcoming is that the cultivation cycle is long, about 3 months; 2, solid fermentation is cultivated the Hericium erinaceus mycelium with solid culture medium, and to obtain secondary metabolite, its advantage is: the simple and wide material sources of medium, low price; Fermentation process does not generally need strict aseptic manipulation; Incubation oxygen supply and temperature can adopt direct force air to ventilate and control; The processing of fermentation residue is simple, directly as feed or fertilizer; Shortcoming is: the medium water activity is low, the inhomogeneous and difficult stirring of medium, and the growth of thalline, all inhomogeneous to the secretion of the absorption of nutriment and metabolite makes the monitoring and the control difficulty of fermentation parameter, so continued operation is very difficult with automation; The temperature control of the heat that microbial respiratory and metabolism are produced is difficulty very; Adopt solid-state cultivation mostly based on empirical data and operating experience, be prone to pollution microbes; 3, liquid deep layer fermenting; Produce a large amount of mycelium and metabolite within a short period of time to obtain optimum operation condition, advantage is: can carry out industrialization and produce continuously, it is extensive to cultivate raw material sources; Reach optimal culture condition through the control installation for fermenting, high with fugitive rate of the cycle of obtaining; Shortcoming is the cycle or longer, and mycelial output is low, is difficult to form large-scale application.
Summary of the invention
The present invention is directed to the deficiency of prior art, propose a kind of medicinal Hericium erinaceus mycelium liquid submerged culturing method for making, this method can be produced the medicinal Hericium erinaceus mycelium of better quality, and method is more perfect.
In order to realize the foregoing invention purpose, the present invention provides following technical scheme: the mycelial cultural method of a kind of medicinal Hericium erinaceus, and may further comprise the steps: the percentage of following substances is percetage by weight,
(1). the optimization screening of bacterial classification and fruit body are separated:
Strong, the fruit body that physically well develops, do not have damage by disease and insect, seed not to be scattered of growth in the wild Hericium erinaceus in the selecting and purchasing Zhongtiao Shan Mountain virgin forest;
At transfer room more than 100 grades in the superclean bench, hericium erinaceus fruiting body immersed in 75% the alcohol and sterilized 1～2 minute, it is clean with distilled water flushing to take out the back; Dry with antiseptic gauze again, fruit body is placed on the culture dish, cover with bell-jar or similar apparatus; Room temperature remains on 20 ℃, and fruit body is slowly grown, excessively after 2～3 days; Seed promptly sheds, and falls into culture dish, dips in tweezers then and gets the seed that falls; Be inoculated in method of scoring on the inclined-plane of test tube and cultivate, cultivate 4～5 days seeds down at 22～25 ℃ and promptly can sprout, when treating that mycelia is covered with the inclined-plane basically; The selection look white, substrate mycelium is flourishing sturdy, and the bacterium bundle significantly is an excellent species, and it is subsequent use to insert shaking table bottle graft kind;
Medium is formed as follows in the culture dish:
The water that adds surplus, 6～7.5,118～121 ℃ of sterilizations of pH value 25～30 minutes;
(2). culture of strains
2.1 preparing culture medium
The water that adds surplus, pH are 6～7.5,118～121 ℃ of sterilizations 28～32 minutes;
2.2 before the inoculation, the inoculation mouth should promptly be wiped examination with the rayon balls of 75% alcohol-pickled mistake earlier with the conventional method sterilization; Unlatching ozone generator in back makes its air outlet all the time facing to the inoculation mouth, around the inoculation mouth, forms a local aseptic area; Then with sterile nontoxic rubber pipe box on the inoculation mouth of pipe, in the shaking table bottle bacterial classification that will pass through spore separation inserted jar, inoculum concentration was 9%～11%; All processes all carries out under aseptic condition, opens agitator after the inoculation, 100 rev/mins of mixing speeds; 25 ℃ of constant temperature were cultivated 3～4 days; The microscopy mycelia is dendroid, does not have assorted bacterium, and outward appearance has the Archon shape;
(3). deep liquid is cultivated:
3.1 seed tank culture
Medium is following the composition:
The water that adds surplus was sterilized 28～32 minutes for 118～121 ℃;
With the cultured bacterial classification inoculation of step (2) to seeding tank, 25～27 ℃, cultivated 3～4 days, to the result be:
1.. microscopy: mycelia is dendroid, does not have assorted bacterium;
2.. outward appearance: more white pelletoid is arranged in the liquid;
3.2 the secondary jar is cultivated
Medium is following the composition:
The water that adds surplus was sterilized 28～32 minutes, the cultured bacterial classification inoculation of step 3.2 to the secondary jar, 25～27 ℃, was cultivated 2～3 days for 118～121 ℃;
3.3 three grades of jars are cultivated
Medium is following the composition:
The water that adds surplus was sterilized 28～32 minutes for 118～121 ℃, with the cultured bacterial classification inoculation to three of step 3.3 grade jar, cultivated 3～4 days for 25～27 ℃, was brown to culture fluid, was full of mycelia, pH value about 4.5, and residual sugar about 0.2% promptly obtains hedgehog fungus mycelium.
Preferably, three grades of hedgehog fungus myceliums that jar obtains, with 8～10 meters spray drying tower, 118～121 ℃ of atomized dryings get the Hericium erinaceus powder.
Compared with prior art, the present invention selects for use by the wild Hericium erinaceus in the Zhongtiao Shan Mountain virgin forest and optimizes the special medicinal white monkey bacterial classification of improveing and cultivating through for many years, adopts liquid submerged femrentation culturing method.The present invention adopts totally-enclosed liquid deep layer fermenting technology to cultivate hericium mycelium; Compare with the artificial plate method of tradition; The production that has not only realized hericium mycelium is by manual breakthrough of cultivating to standardization, suitability for industrialized production; And the production cycle shorten to 14 days by 52 days of traditional cultural method, energy-saving and cost-reducing 30%, production cost reduces by 70%.
The Hericium erinaceus that produces with the method is except that containing the polysaccharide polypeptide; Still also contain five kinds different " callose ", various saccharides such as same glucose, mannitol and galactose combine, and immunocompetence is high; Certain antitumaous effect is arranged, contain vitamin A, vitamin E, Cobastab
1, Cobastab
2, Cobastab
6, Cobastab
12, seven kinds of vitamins such as vitamin C; Contain cellulase, hemicellulase, amylase, guaiaci lignum phenolase and polyphenol oxidase isoreactivity cellulase; And contain trace element of 18 seed amino acids such as leucine, isoleucine, valine, lysine, threonine, methionine, tryptophan, phenyl alanine, histidine, arginine, alanine, asparatate, cystine, glutamic acid, proline, tyrosine, glycine, serine and needed by human etc.; Cystine, histidine, arginine content that wherein medical value is bigger are more; Amino acid is differentiated 6～7 points, protein content 16.65%, is respectively 3 times and 1.8 times of other Hericium erinaceus.Detect also confirmation through infrared spectrum, except that containing the hydroxyl ammoniate, also contain the special anticancer organic acid that does not have in a kind of general hedgehog hydnum.
Embodiment
Describe the present invention below in conjunction with specific embodiment, the description of this part only is exemplary and explanatory, should any restriction not arranged to protection scope of the present invention.
Embodiment 1
A kind of medicinal Hericium erinaceus mycelium liquid submerged culturing method for making, may further comprise the steps: the percentage of following substances is percetage by weight,
(1). the optimization screening of bacterial classification and fruit body are separated:
Strong, the fruit body that physically well develops, do not have damage by disease and insect, seed not to be scattered of growth in the wild Hericium erinaceus in the selecting and purchasing Zhongtiao Shan Mountain virgin forest;
At transfer room more than 100 grades in the superclean bench, hericium erinaceus fruiting body immersed in 75% the alcohol sterilization 2 minutes, it is clean with distilled water flushing to take out the back, dries with antiseptic gauze again; Fruit body is placed on the culture dish, covers with bell-jar or similar apparatus, room temperature remains on 20 ℃, and fruit body is slowly grown; After spending 2 days, seed promptly sheds, and falls into culture dish, dips in tweezers then and gets the seed that falls; Be inoculated in method of scoring on the inclined-plane of test tube and cultivate, cultivate 4 days seeds down at 25 ℃ and promptly can sprout, when treating that mycelia is covered with the inclined-plane basically; The selection look white, substrate mycelium is flourishing sturdy, and the bacterium bundle significantly is an excellent species, and it is subsequent use to insert shaking table bottle graft kind;
Medium is formed as follows in the culture dish:
The water that adds surplus, the pH nature was sterilized 30 minutes for 121 ℃;
(2). culture of strains
2.1 preparing culture medium
The water that adds surplus, the pH nature was sterilized 30 minutes for 121 ℃;
2.2 before the inoculation, the inoculation mouth should promptly be wiped examination with the rayon balls of 75% alcohol-pickled mistake earlier with the conventional method sterilization; Unlatching ozone generator in back makes its air outlet all the time facing to the inoculation mouth, around the inoculation mouth, forms a local aseptic area; Then with sterile nontoxic rubber pipe box on the inoculation mouth of pipe, in the shaking table bottle bacterial classification that will pass through spore separation inserted jar, inoculum concentration was 10%; All processes all carries out under aseptic condition, opens agitator after the inoculation, 100 rev/mins of mixing speeds; 25 ℃ of constant temperature were cultivated 4 days; The microscopy mycelia is dendroid, does not have assorted bacterium, and outward appearance has the Archon shape;
(3). deep liquid is cultivated:
3.1 seed tank culture
Medium is following the composition:
The water that adds surplus was sterilized 30 minutes for 121 ℃;
The cultured bacterial classification inoculation of step (2) to seeding tank, was cultivated 3 days for 26 ℃, to the result is:
1.. microscopy: mycelia is dendroid, does not have assorted bacterium;
2.. outward appearance: more white pelletoid is arranged in the liquid;
3.2 the secondary jar is cultivated
Medium is following the composition:
The water that adds surplus was sterilized 30 minutes, the cultured bacterial classification inoculation of step 3.2 to the secondary jar, 27 ℃, was cultivated 2 days for 121 ℃;
3.3 three grades of jars are cultivated
Medium is following the composition:
The water that adds surplus was sterilized 30 minutes for 121 ℃, with the cultured bacterial classification inoculation to three of step 3.3 grade jar, cultivated 4 days for 25 ℃, was brown to culture fluid, was full of mycelia, pH value about 4.5, and residual sugar about 0.2% promptly obtains hedgehog fungus mycelium.
Three grades of hedgehog fungus myceliums that jar obtains, with 10 meters spray drying tower, 120 ℃ of atomized dryings get the Hericium erinaceus powder.
Embodiment 2
A kind of medicinal Hericium erinaceus mycelium liquid submerged culturing method for making, may further comprise the steps: the percentage of following substances is percetage by weight,
(1). the optimization screening of bacterial classification and fruit body are separated:
Strong, the fruit body that physically well develops, do not have damage by disease and insect, seed not to be scattered of growth in the wild Hericium erinaceus in the selecting and purchasing Zhongtiao Shan Mountain virgin forest;
At transfer room more than 100 grades in the superclean bench, hericium erinaceus fruiting body immersed in 75% the alcohol sterilization 1 minute, it is clean with distilled water flushing to take out the back, dries with antiseptic gauze again; Fruit body is placed on the culture dish, covers with bell-jar or similar apparatus, room temperature remains on 20 ℃, and fruit body is slowly grown; After spending 3 days, seed promptly sheds, and falls into culture dish, dips in tweezers then and gets the seed that falls; Be inoculated in method of scoring on the inclined-plane of test tube and cultivate, cultivate 5 days seeds down at 22 ℃ and promptly can sprout, when treating that mycelia is covered with the inclined-plane basically; The selection look white, substrate mycelium is flourishing sturdy, and the bacterium bundle significantly is an excellent species, and it is subsequent use to insert shaking table bottle graft kind;
Medium is formed as follows in the culture dish:
The water that adds surplus, 7.5,118 ℃ of sterilizations of pH value 30 minutes;
(2). culture of strains
2.1 preparing culture medium
The water that adds surplus, pH are 6,121 ℃ of sterilizations 28 minutes;
2.2 before the inoculation, the inoculation mouth should promptly be wiped examination with the rayon balls of 75% alcohol-pickled mistake earlier with the conventional method sterilization; Unlatching ozone generator in back makes its air outlet all the time facing to the inoculation mouth, around the inoculation mouth, forms a local aseptic area; Then with sterile nontoxic rubber pipe box on the inoculation mouth of pipe, in the shaking table bottle bacterial classification that will pass through spore separation inserted jar, inoculum concentration was 11%; All processes all carries out under aseptic condition, opens agitator after the inoculation, 100 rev/mins of mixing speeds; 25 ℃ of constant temperature were cultivated 3 days; The microscopy mycelia is dendroid, does not have assorted bacterium, and outward appearance has the Archon shape;
(3). deep liquid is cultivated:
3.1 seed tank culture
Medium is following the composition:
The water that adds surplus was sterilized 32 minutes for 118 ℃;
With the cultured bacterial classification inoculation of step (2) to seeding tank, 27 ℃, cultivated 3 days, to the result be:
1.. microscopy: mycelia is dendroid, does not have assorted bacterium;
2.. outward appearance: more white pelletoid is arranged in the liquid;
3.2 the secondary jar is cultivated
Medium is following the composition:
The water that adds surplus was sterilized 28 minutes, the cultured bacterial classification inoculation of step 3.2 to the secondary jar, 25 ℃, was cultivated 3 days for 121 ℃;
3.3 three grades of jars are cultivated
Medium is following the composition:
The water that adds surplus was sterilized 32 minutes for 118 ℃, with the cultured bacterial classification inoculation to three of step 3.3 grade jar, cultivated 3 days for 26 ℃, was brown to culture fluid, was full of mycelia, pH value about 4.5, and residual sugar about 0.2% promptly obtains hedgehog fungus mycelium.
Embodiment 3
A kind of medicinal Hericium erinaceus mycelium liquid submerged culturing method for making, may further comprise the steps: the percentage of following substances is percetage by weight,
(1). the optimization screening of bacterial classification and fruit body are separated:
Strong, the fruit body that physically well develops, do not have damage by disease and insect, seed not to be scattered of growth in the wild Hericium erinaceus in the selecting and purchasing Zhongtiao Shan Mountain virgin forest;
At transfer room more than 100 grades in the superclean bench, hericium erinaceus fruiting body immersed in 75% the alcohol sterilization 2 minutes, it is clean with distilled water flushing to take out the back, dries with antiseptic gauze again; Fruit body is placed on the culture dish, covers with bell-jar or similar apparatus, room temperature remains on 20 ℃, and fruit body is slowly grown; After spending 2 days, seed promptly sheds, and falls into culture dish, dips in tweezers then and gets the seed that falls; Be inoculated in method of scoring on the inclined-plane of test tube and cultivate, cultivate 4 days seeds down at 25 ℃ and promptly can sprout, when treating that mycelia is covered with the inclined-plane basically; The selection look white, substrate mycelium is flourishing sturdy, and the bacterium bundle significantly is an excellent species, and it is subsequent use to insert shaking table bottle graft kind;
Medium is formed as follows in the culture dish:
The water that adds surplus, 6,121 ℃ of sterilizations of pH value 25 minutes;
(2). culture of strains
2.1 preparing culture medium
The water that adds surplus, pH are 7.5,121 ℃ of sterilizations 28 minutes;
2.2 before the inoculation, the inoculation mouth should promptly be wiped examination with the rayon balls of 75% alcohol-pickled mistake earlier with the conventional method sterilization; Unlatching ozone generator in back makes its air outlet all the time facing to the inoculation mouth, around the inoculation mouth, forms a local aseptic area; Then with sterile nontoxic rubber pipe box on the inoculation mouth of pipe, in the shaking table bottle bacterial classification that will pass through spore separation inserted jar, inoculum concentration was 9%; All processes all carries out under aseptic condition, opens agitator after the inoculation, 100 rev/mins of mixing speeds; 25 ℃ of constant temperature were cultivated 4 days; The microscopy mycelia is dendroid, does not have assorted bacterium, and outward appearance has the Archon shape;
(3). deep liquid is cultivated:
3.1 seed tank culture
Medium is following the composition:
The water that adds surplus was sterilized 28 minutes for 121 ℃;
With the cultured bacterial classification inoculation of step (2) to seeding tank, 25 ℃, cultivated 4 days, to the result be:
1.. microscopy: mycelia is dendroid, does not have assorted bacterium;
2.. outward appearance: more white pelletoid is arranged in the liquid;
3.2 the secondary jar is cultivated
Medium is following the composition:
The water that adds surplus was sterilized 32 fens, the cultured bacterial classification inoculation of step 3.2 to the secondary jar, 27 ℃, was cultivated 2 days for 118 ℃;
3.3 three grades of jars are cultivated
Medium is following the composition:
The water that adds surplus was sterilized 28 minutes for 121 ℃, with the cultured bacterial classification inoculation to three of step 3.3 grade jar, cultivated 4 days for 25 ℃, was brown to culture fluid, was full of mycelia, pH value about 4.5, and residual sugar about 0.2% promptly obtains hedgehog fungus mycelium.
The foregoing description 2 and embodiment 3 prepared hedgehog fungus myceliums can adopt equally like embodiment 1 described drying mode and obtain the Hericium erinaceus powder.
Detect the hedgehog fungus mycelium that the foregoing description 1 to embodiment 3 obtains; The result compares with general edible Hericium erinaceus, except that containing the polysaccharide polypeptide, still also contains five kinds different " callose "; Various saccharides such as same glucose, mannitol and galactose combine; Immunocompetence is high, and certain antitumaous effect is arranged, and contains vitamin A, vitamin E, Cobastab
1, Cobastab
2, Cobastab
6, Cobastab
12, seven kinds of vitamins such as vitamin C; Contain cellulase, hemicellulase, amylase, guaiaci lignum phenolase and polyphenol oxidase isoreactivity cellulase; And contain trace element of 18 seed amino acids such as leucine, isoleucine, valine, lysine, threonine, methionine, tryptophan, phenyl alanine, histidine, arginine, alanine, asparatate, cystine, glutamic acid, proline, tyrosine, glycine, serine and needed by human etc.; Cystine, histidine, arginine content that wherein medical value is bigger are more; Amino acid is differentiated 6～7 points, protein content 16.65%, is respectively 3 times and 1.8 times of other Hericium erinaceus.Detect also confirmation through infrared spectrum, except that containing the hydroxyl ammoniate, also contain the special anticancer organic acid that does not have in a kind of general hedgehog hydnum.
The production cycle of above-mentioned each embodiment is 14 ± 2 days simultaneously, more than 52 days cycle weak point of traditional cultural method, saves energy and reduce the cost 30%, and production cost reduces by 70%.
It below only is preferred implementation of the present invention; Should be pointed out that for those skilled in the art, under the prerequisite that does not break away from the principle of the invention; Can also make some improvement and retouching, these improvement and retouching also should be regarded as protection scope of the present invention.
Claims (2)
1. medicinal Hericium erinaceus mycelium liquid submerged culturing method for making, may further comprise the steps: the percentage of following substances is percetage by weight,
(1). the optimization screening of bacterial classification and fruit body are separated:
Strong, the fruit body that physically well develops, do not have damage by disease and insect, seed not to be scattered of growth in the wild Hericium erinaceus in the selecting and purchasing Zhongtiao Shan Mountain virgin forest;
At transfer room more than 100 grades in the superclean bench, hericium erinaceus fruiting body immersed in 75% the alcohol and sterilized 1～2 minute, it is clean with distilled water flushing to take out the back; Dry with antiseptic gauze again, fruit body is placed on the culture dish, cover with bell-jar or similar apparatus; Room temperature remains on 20 ℃, and fruit body is slowly grown, excessively after 2～3 days; Seed promptly sheds, and falls into culture dish, dips in tweezers then and gets the seed that falls; Be inoculated in method of scoring on the inclined-plane of test tube and cultivate, cultivate 4～5 days seeds down at 22～25 ℃ and promptly can sprout, when treating that mycelia is covered with the inclined-plane basically; The selection look white, substrate mycelium is flourishing sturdy, and the bacterium bundle significantly is an excellent species, and it is subsequent use to insert shaking table bottle graft kind;
Medium is formed as follows in the culture dish:
The water that adds surplus, 6～7.5,118～121 ℃ of sterilizations of pH value 25～30 minutes;
(2). culture of strains
2.1 preparing culture medium
The water that adds surplus, pH are 6～7.5,118～121 ℃ of sterilizations 28～32 minutes;
2.2 before the inoculation, the inoculation mouth should promptly be wiped examination with the rayon balls of 75% alcohol-pickled mistake earlier with the conventional method sterilization; Unlatching ozone generator in back makes its air outlet all the time facing to the inoculation mouth, around the inoculation mouth, forms a local aseptic area; Then with sterile nontoxic rubber pipe box on the inoculation mouth of pipe, in the shaking table bottle bacterial classification that will pass through spore separation inserted jar, inoculum concentration was 9%～11%; All processes all carries out under aseptic condition, opens agitator after the inoculation, 100 rev/mins of mixing speeds; 25 ℃ of constant temperature were cultivated 3～4 days; The microscopy mycelia is dendroid, does not have assorted bacterium, and outward appearance has the Archon shape;
(3). deep liquid is cultivated:
3.1 seed tank culture
Medium is following the composition:
The water that adds surplus was sterilized 28～32 minutes for 118～121 ℃;
With the cultured bacterial classification inoculation of step (2) to seeding tank, 25～27 ℃, cultivated 3～4 days, to the result be:
1.. microscopy: mycelia is dendroid, does not have assorted bacterium;
2.. outward appearance: more white pelletoid is arranged in the liquid;
3.2 the secondary jar is cultivated
Medium is following the composition:
The water that adds surplus was sterilized 28～32 minutes, the cultured bacterial classification inoculation of step 3.2 to the secondary jar, 25～27 ℃, was cultivated 2～3 days for 118～121 ℃;
3.3 three grades of jars are cultivated
Medium is following the composition:
The water that adds surplus was sterilized 28～32 minutes for 118～121 ℃, with the cultured bacterial classification inoculation to three of step 3.3 grade jar, cultivated 3～4 days for 25～27 ℃, was brown to culture fluid, was full of mycelia, pH value about 4.5, and residual sugar about 0.2% promptly obtains hedgehog fungus mycelium.
2. cultural method according to claim 1 is characterized in that: three grades of hedgehog fungus myceliums that jar obtains, with 8～10 meters spray drying tower, 118～121 ℃ of atomized dryings, the Hericium erinaceus powder.
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| CN103416222A (en) * | 2013-07-31 | 2013-12-04 | 常州大学 | Lepista nuda spore cultivation and ripening method through hypha shake flasks and liquid submerged fermentation process |
| CN104224855A (en) * | 2013-06-06 | 2014-12-24 | 湖南新汇制药股份有限公司 | Use of hericium erinaceus hyphae extract in preparation of drug for resisting stomach cancer |
| CN104824767A (en) * | 2014-02-12 | 2015-08-12 | 于汇 | Hericium erinaceus fermentation appetite-enhancing and digestion-promoting beverage |
| CN106376354A (en) * | 2016-08-29 | 2017-02-08 | 浙江省医学科学院 | Hericium erinaceus polysaccharide extraction raw material preparation method |
| CN106727730A (en) * | 2016-12-31 | 2017-05-31 | 健务（上海）生物科技有限公司 | Adjust biological bacterium solution of immunity of organisms anticancer and preparation method thereof |
| WO2017117701A1 (en) * | 2016-01-04 | 2017-07-13 | 葡萄王生技股份有限公司 | Active substance for preventing degeneration of hearing, composition comprising same, and preparation method thereof |
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| CN103305429A (en) * | 2013-06-26 | 2013-09-18 | 东北林业大学 | Method for collecting liquid culture fungal mycelium |
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| CN104824767A (en) * | 2014-02-12 | 2015-08-12 | 于汇 | Hericium erinaceus fermentation appetite-enhancing and digestion-promoting beverage |
| US10405504B2 (en) | 2016-01-04 | 2019-09-10 | Grape King Bio Ltd. | Active substances for preventing hearing deterioration, the composition containing the active substances, and the preparation method thereof |
| WO2017117701A1 (en) * | 2016-01-04 | 2017-07-13 | 葡萄王生技股份有限公司 | Active substance for preventing degeneration of hearing, composition comprising same, and preparation method thereof |
| CN106376354A (en) * | 2016-08-29 | 2017-02-08 | 浙江省医学科学院 | Hericium erinaceus polysaccharide extraction raw material preparation method |
| CN106727730A (en) * | 2016-12-31 | 2017-05-31 | 健务（上海）生物科技有限公司 | Adjust biological bacterium solution of immunity of organisms anticancer and preparation method thereof |
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| CN109906876B (en) * | 2019-03-20 | 2021-10-15 | 湖北民族学院 | Selenium-rich hericium erinaceus mycelium, preparation method and application thereof |
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