CN104987957A - Preparation method for laundry detergent based on fish intestine proteinase - Google Patents
Preparation method for laundry detergent based on fish intestine proteinase Download PDFInfo
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- CN104987957A CN104987957A CN201510405805.9A CN201510405805A CN104987957A CN 104987957 A CN104987957 A CN 104987957A CN 201510405805 A CN201510405805 A CN 201510405805A CN 104987957 A CN104987957 A CN 104987957A
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Abstract
The invention belongs to the field of detergent mixtures, in particular relates to a preparation method of a laundry detergent based on fish intestine proteinase. The preparation method comprises the following steps of dissecting and taking the fish intestine, performing cleaning treatment, homogenate treatment, extracting treatment and centrifugal treatment to obtain fish intestine proteinase hydrolytic enzymes; mixing the fish intestine proteinase hydrolytic enzymes, surfactant, thickener, essence and water uniformly to obtain the fish intestine proteinase laundry detergent. the natural fish intestine is taken as a main raw material, active protease is extracted from viscera wastes of fish, and the clean and efficient fish intestine proteinase laundry detergent is produced; according to the preparation method provided by the invention, various raw materials are in reasonable proportion, good decontamination effect is realized, and no harm to human bodies and the environment.
Description
Technical field
The invention belongs to detergent mixture field, particularly a kind of preparation method based on fish ereptase washing liquid, with natural fish intestines for main raw material, there is good clean effect.
Background technology
There is the enterprise of the series products that cures fish in a large number China's Coastal Areas, has tens thousand of ton to discard fish intestines every year and is introduced into sea.This discarded fish intestines are not yet effectively utilized, not only contaminate environment, and serious waste resource.Early document is had to point out, the major ingredient of discarded fish intestines is aid digestion enzymes, as all enzymes, this is a kind of biological catalyst accelerating biochemical reaction speed, because this material can decomposing protein be so be called as proteolytic ferment effectively, it can be used as the protein in a kind of excellent clean-out system removal dirt.
At present, enzyme-containing detergent is the Sumizyme MP adopting mushroom to turn out mostly, and due to resource-constrained, culturing process is loaded down with trivial details, greatly have impact on the output of enzyme.Chinese patent application " biological environmental production multifunction special effect washing liquid and preparation method thereof " (application number: 201110403398.X, publication No.: CN 102517177A) disclose a kind of biological environmental production multifunction special effect washing liquid, containing alkyl glycoside, Oleum Cocois, Sumizyme MP, Seed of Chinese Soapberry soap breast, matrine, natural soap powder, Oleum Cocois LAO, disodium ethylene diamine tetraacetate, auxiliary agent, sanitas, essence, citric acid, adjust ph is 6.5 ~ 7.5.The defect that this washing liquid exists is: adopt Sumizyme MP, fill a prescription more complicated, and complex process, production cost is high, and part material adopts chemical feedstocks, be unfavorable for water treatment and the environment protection in later stage, and this washing liquid detersive power is limited.
Therefore, market needs a kind of utilize discarded fish intestines as raw-material, clean effect good and environment amenable washing liquid.
Summary of the invention
For the deficiencies in the prior art, the object of the present invention is to provide a kind of based on fish ereptase washing liquid, raw material of the present invention is natural fish intestines, has good clean effect, environmentally friendly.
The object of the invention is to be achieved through the following technical solutions:
Based on a preparation method for fish ereptase washing liquid, comprise the steps:
Protease hydrolysis liquid preparation process: take fish intestines, carries out clean, homogenized, extraction process, centrifugal treating, obtains fish Erepsin lytic enzyme liquid;
Washing liquid preparation steps: described fish Erepsin lytic enzyme liquid is mixed with tensio-active agent, thickening material, essence, water, obtains fish ereptase washing liquid.
Above-mentioned preparation method preferred embodiment in, described fish intestines are selected from wherein one or more such as small yellow croaker, large yellow croaker, snakeheaded fish, squid, mackerel, cod, carp, grass carp, swamp eel.
Above-mentioned preparation method preferred embodiment in, in described homogenized, in fish intestines, add water, the weight ratio of fish intestines and water is 1:(2-4); The temperature of described homogenized is 0-4 DEG C, and the time is 20-70s.
Above-mentioned preparation method preferred embodiment in, between described homogenized and described extraction process, also carry out centrifugal treating, rotating speed is 8000-14000r/min, and the time is 20-50min, retain supernatant liquor.
Above-mentioned preparation method preferred embodiment in, in described extraction process, the extraction solution of employing is propyl carbinol.
Above-mentioned preparation method preferred embodiment in, in described extraction process, the volume ratio of sample to be extracted and described extraction solution is 1:(2-5); Temperature is 0-4 DEG C, and the time is 2-5h.
Above-mentioned preparation method preferred embodiment in, in described centrifugal treating, rotating speed is 8000-14000r/min, and the time is 20-50min, and temperature is 0-4 DEG C.
Above-mentioned preparation method preferred embodiment in, the ratio of weight and number of described tensio-active agent, thickening material, essence, fish Erepsin lytic enzyme liquid, water is:
Above-mentioned preparation method preferred embodiment in, described tensio-active agent is sulfonated α-olefin, polyoxyethylene alkyl ether sulfate salt, fatty alcohol-polyoxyethylene ether, linear alkylbenzene sulphonic acid, fatty alcohol sulphuric acid (ester) salt, the combination of one or more wherein of alkylphosphonic acid carboxylic acid list (two) ester salt.
Above-mentioned preparation method preferred embodiment in, described thickening material is the combination of ethanol, glycerine, ethylene glycol, sodium xylene sulfonate wherein one or more.
The present invention has following beneficial effect compared to existing technology:
1, the present invention is with natural fish intestines for main raw material, extracts active protease, produce clean, efficient fish ereptase washing liquid from the internal organ waste of fish.
2, various raw material rational proportion of the present invention, has good clean effect, and to human body and environment all harmless.
3, the present invention can improve the efficiency of utilization of waste material, China's Coastal Areas, has the enterprise of the series products that cures fish in a large number every year, and discarded fish intestines just can be utilized to carry out production proteolytic enzyme, synthesis fish ereptase washing liquid.
4, product of the present invention has efficiently, the decontamination dirt features such as ability is strong, and starting material are sufficient, and environmental protection is renewable, efficiently utilizes fishery products waste, has started fish protein enzyme and has been applied to method in washing liquid, had wide market and prospect.
Embodiment
Based on a preparation method for fish ereptase washing liquid, comprise the following steps:
The extraction of step one, proteolytic ferment liquid: select suitable fish (wherein one or more such as small yellow croaker, large yellow croaker, snakeheaded fish, squid, mackerel, cod, carp, grass carp, swamp eel), take fish intestines; Again broken for fish intestinal scissors rear deionized water is carried out clean, obtain the fish intestines after cleaning;
Again the fish intestines after cleaning are added deionized water and carry out homogenized in high-speed homogenization machine, obtain fish intestines homogenate, the weight ratio of fish intestines and deionized water is 1:(2-4) (exemplarily, can be 1:2, 1:2.5, 1:3, 1:3.5, arbitrary value or any scope between the two in 1:4 etc.), homogenized temperature be 0-4 DEG C (exemplarily, can be 0 DEG C, 1 DEG C, 2 DEG C, 3 DEG C, arbitrary value or any scope between the two in 4 DEG C of grades), time be 20-70s (exemplarily, can be 20s, 35s, 50s, 60s, arbitrary value or any scope between the two in 70s etc.),
Carry out centrifugal treating to fish intestines homogenate again, rotating speed is 8000-14000r/min, and the time is 20-50min, retains supernatant liquor;
Again the propyl carbinol adding 4 DEG C in the supernatant liquor after centrifugal is carried out extraction process, the volume ratio of this supernatant liquor and Extraction solvent is 1:(2-5) (exemplarily, can be 1:2, 1:2.5, 1:3, 1:3.5, 1:4, arbitrary value or any scope between the two in 1:5 etc.), Extracting temperature be 0-4 DEG C (exemplarily, can be 0 DEG C, 1 DEG C, 2 DEG C, 3 DEG C, arbitrary value or any scope between the two in 4 DEG C of grades), time be 2-5h (exemplarily, can be 2h, 2.5h, 3h, 4h, arbitrary value or any scope between the two in 5h etc.), obtain fish intestines extracting solution,
Carry out centrifugal treating to fish intestines extracting solution again, rotating speed is 8000-14000r/min, and the time is 20-50min, and temperature is 0-4 DEG C, retains supernatant liquor, obtains fish Erepsin lytic enzyme liquid.
Exemplarily, rotating speed in above-mentioned two times centrifugal process can be arbitrary value or any scope between the two in 8000r/min, 9000r/min, 10000r/min, 11000r/min, 12000r/min, 14000r/min etc., and the time can be arbitrary value or any scope between the two in 20min, 30min, 35min, 40min, 50min etc.
This washing liquid for main raw material with natural fish intestines, extracts active protease from the internal organ waste of fish, produces clean, efficient fish ereptase washing liquid.
The preparation of step 2, washing liquid: mixed with tensio-active agent, thickening material, essence, distilled water by this fish Erepsin lytic enzyme liquid, repeats to stir, obtains fish ereptase washing liquid.
Wherein, the ratio of weight and number of each raw material above-mentioned is as follows:
Exemplarily, the ratio of weight and number of above-mentioned tensio-active agent, thickening material, essence, fish Erepsin lytic enzyme liquid, distilled water also can be the arbitrary value in 18:0.8:0.5:10:55,35:2.5:0.5:20:70,18:3.4:0.5:10:70,25:2:0.5:15:55,20:3.4:2.5:10:55,30:1:1:15:70 etc.
Above-mentioned tensio-active agent is wherein one or more such as sulfonated α-olefin (AOS), polyoxyethylene alkyl ether sulfate salt (AES), fatty alcohol-polyoxyethylene ether (AEO), linear alkylbenzene sulphonic acid (LAS), fatty alcohol sulphuric acid (ester) salt, alkylphosphonic acid carboxylic acid list (two) ester salt;
Above-mentioned thickening material is ethanol, glycerine, ethylene glycol, sodium xylene sulfonate wherein one or more.
The various raw material rational proportions of this washing liquid, have good clean effect, and to human body and environment all harmless.
Step 3, packaging: fish ereptase washing liquid is packed, gets product.
Below by way of specific embodiment, the present invention is described in detail, but these exemplary embodiments not intended to be form any type of any restriction to real protection scope of the present invention.
Embodiment 1
The present embodiment the preparation method of fish ereptase washing liquid is comprised the steps:
(1) pre-treatment of fish intestines: get small yellow croaker fish intestines, shred with scissors, with distilled water cleaning bloodstain and foreign matter, obtains the fish intestines after cleaning, weighs.
(2) homogenate: the deionized water mixing of getting fish intestines 60g and the 120mL after cleaning, joins homogenate 30s in high-speed homogenization machine, obtain fish intestines homogenate.
(3) extract: by this fish intestines homogenate at 4 DEG C, under 12000r/min rotating speed, centrifugal 30min, gets supernatant liquor; Measure this supernatant liquor of 60ml, add 120ml propyl carbinol (4 DEG C), place 5h in 4 DEG C and extract, obtain fish intestines extracting solution.
(4) centrifugal: by fish intestines extracting solution in 4 DEG C, under 12000r/min rotating speed, frozen centrifugation 30min, gets supernatant, namely obtain fish Erepsin lytic enzyme liquid (crude enzyme liquid).
(5) washing liquid preparation: get above-mentioned fish Erepsin lytic enzyme liquid 100mL, add sulfonated α-olefin (AOS) 100mL, ethanol 10mL, essence 3.5g, add deionized water to 500mL, stir, obtain fish ereptase washing liquid.
(6) produce and pack: above-mentioned fish ereptase washing liquid being contained in the container tank of the certain volume designed, packs, get product.
Embodiment 2
The present embodiment the preparation method of fish ereptase washing liquid is comprised the steps:
(1) pre-treatment of fish intestines: get small yellow croaker fish intestines, shred with scissors, with distilled water cleaning bloodstain and foreign matter, obtains the fish intestines after cleaning, weighs.
(2) homogenate: the deionized water mixing of getting fish intestines 60g and the 120mL after cleaning, joins homogenate 40s in high-speed homogenization machine, obtain fish intestines homogenate.
(3) extract: by this fish intestines homogenate at 4 DEG C, under 10000r/min rotating speed, centrifugal 30min, gets supernatant liquor; Measure this supernatant liquor of 60ml, add 120ml propyl carbinol (4 DEG C), place 5h in 4 DEG C and extract, obtain fish intestines extracting solution.
(4) centrifugal: by fish intestines extracting solution in 4 DEG C, under 12000r/min rotating speed, frozen centrifugation 30min, gets supernatant, namely obtain fish Erepsin lytic enzyme liquid (crude enzyme liquid).
(5) washing liquid preparation: get above-mentioned fish Erepsin lytic enzyme liquid 100mL, add fatty alcohol-polyoxyethylene ether (AEO) 115mL, ethanol 10mL, essence 5g, add deionized water to 500mL, stir, obtain fish ereptase washing liquid.
(6) produce and pack: above-mentioned fish ereptase washing liquid being contained in the container tank of the certain volume designed, packs, get product.
Embodiment 3
The present embodiment the preparation method of fish ereptase washing liquid is comprised the steps:
(1) pre-treatment of fish intestines: get small yellow croaker fish intestines, shred with scissors, with distilled water cleaning bloodstain and foreign matter, obtains the fish intestines after cleaning, weighs.
(2) homogenate: the deionized water mixing of getting fish intestines 60g and the 150mL after cleaning, joins homogenate 50s in high-speed homogenization machine, obtain fish intestines homogenate.
(3) extract: by this fish intestines homogenate at 4 DEG C, under 12000r/min rotating speed, centrifugal 40min, gets supernatant liquor; Measure this supernatant liquor of 60ml, add 150ml propyl carbinol (4 DEG C), place 3h in 4 DEG C and extract, obtain fish intestines extracting solution.
(4) centrifugal: by fish intestines extracting solution in 4 DEG C, under 10000r/min rotating speed, frozen centrifugation 30min, gets supernatant, namely obtain fish Erepsin lytic enzyme liquid (crude enzyme liquid).
(5) washing liquid preparation: get above-mentioned fish Erepsin lytic enzyme liquid 100mL, add fatty alcohol sulphuric acid (ester) salt 115mL, ethylene glycol 10mL, essence 8g, add deionized water to 500mL, stir, and obtains fish ereptase washing liquid.
(6) produce and pack: above-mentioned fish ereptase washing liquid being contained in the container tank of the certain volume designed, packs, get product.
Embodiment 4
The present embodiment the preparation method of fish ereptase washing liquid is comprised the steps:
(1) pre-treatment of fish intestines: get small yellow croaker fish intestines, shred with scissors, with distilled water cleaning bloodstain and foreign matter, obtains the fish intestines after cleaning, weighs.
(2) homogenate: the deionized water mixing of getting fish intestines 60g and the 140mL after cleaning, joins homogenate 30s in high-speed homogenization machine, obtain fish intestines homogenate.
(3) extract: by this fish intestines homogenate at 4 DEG C, under 12000r/min rotating speed, centrifugal 40min, gets supernatant liquor; Measure this supernatant liquor of 60ml, add 140ml propyl carbinol (4 DEG C), place 3h in 4 DEG C and extract, obtain fish intestines extracting solution.
(4) centrifugal: by fish intestines extracting solution in 4 DEG C, under 10000r/min rotating speed, frozen centrifugation 40min, gets supernatant, namely obtain fish Erepsin lytic enzyme liquid (crude enzyme liquid).
(5) washing liquid preparation: get above-mentioned fish Erepsin lytic enzyme liquid 120mL, add alkylphosphonic acid carboxylic acid list (two) ester salt 150mL, ethanol 10mL, essence 7.5g, add deionized water to 500mL, stir, and obtains fish ereptase washing liquid.
(6) produce and pack: above-mentioned fish ereptase washing liquid being contained in the container tank of the certain volume designed, packs, get product.
Embodiment 5
The present embodiment the preparation method of fish ereptase washing liquid is comprised the steps:
(1) pre-treatment of fish intestines: get small yellow croaker fish intestines, shred with scissors, with distilled water cleaning bloodstain and foreign matter, obtains the fish intestines after cleaning, weighs.
(2) homogenate: the deionized water mixing of getting fish intestines 60g and the 120mL after cleaning, joins homogenate 30s in high-speed homogenization machine, obtain fish intestines homogenate.
(3) extract: by this fish intestines homogenate at 4 DEG C, under 12000r/min rotating speed, centrifugal 40min, gets supernatant liquor; Measure this supernatant liquor of 60ml, add 120ml propyl carbinol (4 DEG C), place 3h in 4 DEG C and extract, obtain fish intestines extracting solution.
(4) centrifugal: by fish intestines extracting solution in 4 DEG C, under 10000r/min rotating speed, frozen centrifugation 30min, gets supernatant, namely obtain fish Erepsin lytic enzyme liquid (crude enzyme liquid).
(5) washing liquid preparation: get above-mentioned fish Erepsin lytic enzyme liquid 100mL, add linear alkylbenzene sulphonic acid (LAS) 135mL, glycerine 10mL, essence 7.5g, add deionized water to 500mL, stir, obtain fish ereptase washing liquid.
(6) produce and pack: above-mentioned fish ereptase washing liquid being contained in the container tank of the certain volume designed, packs, get product.
Test example
In order to detect the washing effect of fish ereptase washing liquid, do experiment as follows:
1, get one piece of clean calico, be cut into the square of 6cm × 6cm.
2, cloth is placed on soaks in spot liquid, and dry, spot liquid edible soy sauce.
3, measure washing liquid 2mL prepared by above embodiment 1-5 respectively, be placed in different large beakers respectively, all add the tap water of 200mL, glass stick stirs and washing liquid composition is dissolved completely.(control group: do not add washing liquid in large beaker only adds 200mL tap water.)
4, the cloth of preparation in step 2 is put into large beaker, soak 25 minutes, stir 10 minutes with glass stick.
5, take out cloth, with tap water rinse 2 times, dewater, dry.
6, with the whiteness value of blancometer test cloth before and after washing, evaluate washing effect, whiteness value is higher, then illustrate that washing effect is better.Get 5 tests at every block soiled cotton pros and cons, mean value is whiteness value W, and is calculated as follows decontamination value S (%).
Wherein W
0for cloth pollutes front whiteness value; W
1for cloth stains rear whiteness value; W
2for staining the rear whiteness value of cloth washing.
Test result: as following table
| Project | W 0 | W 1 | W 2 | S |
| Embodiment 1 | 70.4 | 12.3 | 39.8 | 47.3 |
| Embodiment 2 | 70.6 | 12.5 | 40.3 | 47.8 |
| Embodiment 3 | 70.1 | 11.9 | 40.2 | 48.6 |
| Embodiment 4 | 70.8 | 12.3 | 40.7 | 48.6 |
| Embodiment 5 | 70.4 | 12.6 | 40.3 | 47.9 |
| Blank | 70.6 | 11.8 | 17.9 | 10.4 |
To sum up test-results can obtain, and the detersive power of the fish ereptase washing liquid that embodiment 1-5 obtains is obvious, and decontamination value S is greater than 47%, and washing effect is stronger.
Should be appreciated that the purposes of these case study on implementation is only not intended to for illustration of the present invention limit the scope of the invention.In addition; also should understand; after having read technology contents of the present invention, those skilled in the art can make various change, amendment and modification to the present invention, and these all equivalent form of values fall within the protection domain that the application's appended claims limits equally.
Claims (10)
1. based on a preparation method for fish ereptase washing liquid, it is characterized in that: described method comprises the steps:
Protease hydrolysis liquid preparation process: take fish intestines, carries out clean, homogenized, extraction process, centrifugal treating, obtains fish Erepsin lytic enzyme liquid;
Washing liquid preparation steps: described fish Erepsin lytic enzyme liquid is mixed with tensio-active agent, thickening material, essence, water, obtains fish ereptase washing liquid.
2., according to claim 1 based on the preparation method of fish ereptase washing liquid, it is characterized in that:
Described fish intestines are selected from one or more in small yellow croaker, large yellow croaker, snakeheaded fish, squid, mackerel, cod, carp, grass carp, swamp eel.
3., according to claim 1 based on the preparation method of fish ereptase washing liquid, it is characterized in that:
In described homogenized, in fish intestines, add water, the weight ratio of fish intestines and water is 1:(2-4); The temperature of described homogenized is 0-4 DEG C, and the time is 20-70s.
4. according to claim 1 or 3 based on the preparation method of fish ereptase washing liquid, it is characterized in that:
Between described homogenized and described extraction process, also carry out centrifugal treating, rotating speed is 8000-14000r/min, and the time is 20-50min, retains supernatant liquor.
5., according to claim 1 based on the preparation method of fish ereptase washing liquid, it is characterized in that:
In described extraction process, the extraction solution of employing is propyl carbinol.
6. according to claim 1 or 5 based on the preparation method of fish ereptase washing liquid, it is characterized in that:
In described extraction process, the volume ratio of sample to be extracted and described extraction solution is 1:(2-5); Temperature is 0-4 DEG C, and the time is 2-5h.
7., according to claim 1 based on the preparation method of fish ereptase washing liquid, it is characterized in that:
In described centrifugal treating, rotating speed is 8000-14000r/min, and the time is 20-50min, and temperature is 0-4 DEG C.
8., according to claim 1 based on the preparation method of fish ereptase washing liquid, it is characterized in that:
The ratio of weight and number of described tensio-active agent, thickening material, essence, fish Erepsin lytic enzyme liquid, water is:
9. according to claim 1 or 8 based on the preparation method of fish ereptase washing liquid, it is characterized in that:
Described tensio-active agent is the combination of one or more in sulfonated α-olefin, polyoxyethylene alkyl ether sulfate salt, fatty alcohol-polyoxyethylene ether, linear alkylbenzene sulphonic acid, fatty alcohol sulphuric acid (ester) salt, alkylphosphonic acid carboxylic acid list (two) ester salt.
10. according to claim 1 or 8 based on the preparation method of fish ereptase washing liquid, it is characterized in that:
Described thickening material is the combination of one or more in ethanol, glycerine, ethylene glycol, sodium xylene sulfonate.
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| CN104232339A (en) * | 2014-09-30 | 2014-12-24 | 郑通彪 | Low foam liquid detergent |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104232339A (en) * | 2014-09-30 | 2014-12-24 | 郑通彪 | Low foam liquid detergent |
Non-Patent Citations (2)
| Title |
|---|
| 何乃久: "从废弃鱼肠中提取蛋白酶", 《水产科技情报》 * |
| 吴燕燕等: "罗非鱼内脏蛋白酶超声波提取工艺的研究", 《食品科学》 * |
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