CN104938538A - Plant-derived pesticide and production method thereof - Google Patents
Plant-derived pesticide and production method thereof Download PDFInfo
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- CN104938538A CN104938538A CN201510303796.2A CN201510303796A CN104938538A CN 104938538 A CN104938538 A CN 104938538A CN 201510303796 A CN201510303796 A CN 201510303796A CN 104938538 A CN104938538 A CN 104938538A
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Abstract
The invention discloses plant-derived pesticide and a production method thereof. The plant-derived pesticide is prepared from Chinese herbal medicines such as licorice root, phellodendron bark, melia azedarach, cockscomb, radix ranunculi ternati, silktree flower, butterflybush flower, salvia chinensis, fenugreek seed and golden larch bark. A field test proves that the plant-derived pesticide has tobacco black shank prevention and treatment efficiency of 80% or more and has obvious effects better than that of metalaxyl with a concentration of 25%. The plant-derived pesticide has substantial prevention and treatment effects on a plurality of fungous diseases such as downy mildew, sclerotinia rot, powdery mildew, botrytis, anthracnose, epidemic disease, black shank and sheath blight of crops such as fruits, vegetables and paddy rice.
Description
Technical field
The present invention relates to a kind of agricultural chemicals and production method, particularly relate to a kind of botanical pesticide and production method.
Background technology
At present in disease such as control fruit, vegetables etc., adopt Agro-chemicals control more, the use of chemical pesticide, make various harmful levels of pathogens create drug resistance undoubtedly, add difficulty of prevention and cure, simultaneously, also harm is created to plant itself, further, have residual in vegetable melon and fruit, harm health of people, contaminated environment, the destruction ecological balance.And botanical pesticide is as the important component part of biopesticide, there is efficient, low toxicity or nontoxic, that low-residual, selectivity are high advantage, paid attention to more and more widely.Along with the development of botanical pesticide, Chinese herbal medicine source pesticide becomes the emphasis of botanical pesticide research gradually.The botanical pesticide that applicant obtains through effort for many years, through field trial, the control efficiency had.
Summary of the invention
The object of this invention is to provide a kind of noresidue, non-environmental-pollution, control efficiency a kind of botanical pesticide that is remarkable, cheap, that be made up of pure Chinese herbal medicine.
The object of the invention is to be achieved through the following technical solutions, this botanical pesticide is made up of the herbal raw material of following weight portion: Radix Glycyrrhizae 12-16 part, phellodendron bark 6-10 part, China tree 6-10 part, cockscomb 6-10 part, radix ranunculi ternati 6-10 part, Flos Albiziae 4-8 part, butterflybush flower 4-8 part, salvia chinensis 2-6 part, reed Ba Zi 2-6 part, Golden Larch Bark 2-6 part.
Preferred: 14 parts, Radix Glycyrrhizae, phellodendron bark 8 parts, China tree 8 parts, cockscomb 8 parts, radix ranunculi ternati 8 parts, Flos Albiziae 6 parts, butterflybush flower 6 parts, salvia chinensis 4 parts, reed Ba Zi 4 parts, Golden Larch Bark 4 parts.
Preparation method: produce by traditional ethanol extraction method.
The control efficiency of this botanical pesticide to the multiple fungal disease such as downy mildew, stalk break, powdery mildew, gray mold, anthracnose, epidemic disease, balck shank, banded sclerotial blight of the crops such as melon and fruit, vegetables, paddy rice is remarkable.
Field trial
The preparation of test plant source pesticide: take 140 grams, Radix Glycyrrhizae respectively, phellodendron bark 80 grams, China tree 80 grams, cockscomb 80 grams, radix ranunculi ternati 80 grams, Flos Albiziae 60 grams, butterflybush flower 60 grams, salvia chinensis 40 grams, reed Ba Zi 40 grams, Golden Larch Bark 40 grams, grind into powder and mix, first cross 60 mesh sieves, rear mistake 100 mesh sieve, Chinese herbal medicine powder after sieving, in the ratio of 20g with 80mL80% ethanol, put into container after proportioning is good to seal, put into shaken cultivation case to vibrate it under the condition of 30 DEG C of 150 r/min after 24h through 3 layers of filtered through gauze, centrifugal 10min under 2500r/min condition, discard sediment, under 45 degree, with Rotary Evaporators, supernatant is carried out reduced pressure concentration, obtain more sticky ethanol concentrate, concentration is made to be lg/mL, obtain this botanical pesticide.
Above-mentioned obtained botanical pesticide is diluted respectively 100 times, 200 times, 300 times liquid, compare, carry out field control effectiveness test to black shank with 25% metalaxyl and clear water, so that beneficial effect of the present invention to be described better, result of the test is as shown in following table 1.
Experimental field be positioned at tobacco planting base, Linyi City Yinan County, black shank occur, test in first 6 days and process of the test and do not use other agricultural chemicals.Experimental field soil property is sandy loam, and rich water is medium, and management is consistent.Duration of test temperature at 25 ~ 31 degrees Celsius, mean temperature 28 degrees Celsius; This botanical pesticide 100 times of liquid, 200 times of liquid, 300 times of liquid are established in this field trial; 25% metalaxyl; Clear water (CK).Establish 5 process altogether, each process repetition 3 times, totally 12 process communities, often process community according to the medicament preparation listed by table 1, select growing way and the basically identical field of morbidity to start phase the 1st medication in black shank, after this every 7d medication 1 time, continuous 2 times, use knapsack sprayer, amount of liquid medicine standard is 30kg/ mu, every strain blade and stem by medicine evenly, avoid liquid to drift about.After first time dispenser, second time medicine is executed at interval after 7 days, altogether dispenser twice.After each dispenser, the 7th afternoon unified investigation tobacco stem scab number, lesion area, calculate disease index, control efficiency.4 points are evenly fixed in Cha Shimei community, often 4 strains, totally 16 strains.
Drug effect standard: scab grade scale is as follows.Stem occurring degree is divided into 5 grades, black shank Seriousness gradation standard: 0 grade, stem is without scab; 1 grade, scab accounts for less than 5% of full stem area; 2 grades, scab accounts for 6% ~ 10% of full stem area; 3 grades, scab accounts for 11% ~ 25% of full stem area; 4 grades, scab accounts for 26% ~ 50% of full stem area; 5 grades, scab accounts for more than 50% of full stem area.Disease refers to calculate as follows with control efficiency:
Table 1 different disposal is to the control efficiency of black shank
Conclusive table 1 result of the test can draw, after twice dispenser, this botanical pesticide is to the control efficiency of black shank all more than 68%, and successful, is better than the metalaxyl of 25%.Botanical pesticide 3 various dose process along with the increase of spraying times is in rising trend, reach good control efficiency, and medicament efficiency time are long to the control efficiency of black shank.Experimental session is observed, and botanical pesticide is to tobacco plants without poisoning, and safety is good.The above results shows this botanical pesticide Be very effective in control black shank.By above method of testing, we test the downy mildew of crop, stalk break, powdery mildew, gray mold, anthracnose, epidemic disease, banded sclerotial blight, all achieve good control efficiency.
Biologicall test
:
Adopt mycelial growth rate method biometric techniques.Crude extract acetone solution, measures tobacco black shank bacterium to the susceptibility of acetone, measures the content of acetone suppressing mycelia less than 20%, thus control acetone concentration in biologicall test, the impact of acetone on biological activity determination is reduced as far as possible before biologicall test.
Botanical pesticide of the present invention more as seen from Table 2, is set to 1.5g/L and 2.5g/L two kinds of concentration, respectively at 5d and 7d " Invest, Then Investigate " colony growth diameter, then calculates the bacteriostasis rate to tobacco black shank bacterium by botanical pesticide antibacterial activity of the present invention.
| Botanical pesticide of the present invention | 5 days inhibiting rates | 7 days inhibiting rates |
| 1.5g/L | 60.2% | 75.9% |
| 2.5g/L | 65.7% | 82.3% |
Result shows that botanical pesticide antibacterial effect of the present invention is good, and when 1.5g/L and 2.5g/L, the inhibiting rate of 5d is respectively 60.2% and 65.7%; The inhibiting rate of 7d is respectively 75.9% and 82.3%, result of study shows that the medicament bacteriostasis rate of two kinds of variable concentrations is all more than 60%, and along with concentration, administration time increase, fungistatic effect has reinforcement, botanical pesticide of the present invention is respond well in suppression tobacco black shank bacterium as can be seen here.
By above method of testing, we test the downy mildew of crop, stalk break, powdery mildew, gray mold, anthracnose, epidemic disease, banded sclerotial blight, all achieve good control efficiency.
Embodiment
Embodiment one: take 140 grams, Radix Glycyrrhizae, phellodendron bark 80 grams, China tree 80 grams, cockscomb 80 grams, radix ranunculi ternati 80 grams, Flos Albiziae 60 grams, butterflybush flower 60 grams, salvia chinensis 40 grams, reed Ba Zi 40 grams, Golden Larch Bark 40 grams, grind mixing into powder, first cross 60 mesh sieves, rear mistake 100 mesh sieve, Chinese herbal medicine powder after sieving, in the ratio of 20g with 80mL80% ethanol, put into container after proportioning is good to seal, put into shaken cultivation case to vibrate it under the condition of 30 DEG C of 150 r/min after 24h through 3 layers of filtered through gauze, centrifugal 10min under 2500r/min condition, discard sediment, under 45 degree, with Rotary Evaporators, supernatant is carried out reduced pressure concentration, obtain more sticky ethanol concentrate, concentration is made to be lg/mL, obtain this botanical pesticide, be put in refrigerator for subsequent use.
Embodiment two: take 150 grams, Radix Glycyrrhizae, phellodendron bark 90 grams, China tree 90 grams, cockscomb 90 grams, radix ranunculi ternati 90 grams, Flos Albiziae 70 grams, butterflybush flower 70 grams, salvia chinensis 50 grams, reed Ba Zi 50 grams, Golden Larch Bark 50 grams.Preparation method is with embodiment one.
Embodiment three: take 130 grams, Radix Glycyrrhizae, phellodendron bark 70 grams, China tree 70 grams, cockscomb 70 grams, radix ranunculi ternati 70 grams, Flos Albiziae 50 grams, butterflybush flower 50 grams, salvia chinensis 30 grams, reed Ba Zi 30 grams, Golden Larch Bark 30 grams.Preparation method is with embodiment one.
Claims (2)
1. botanical pesticide and a production method, is characterized in that this botanical pesticide is made up of the herbal raw material of following weight portion: Radix Glycyrrhizae 12-16 part, phellodendron bark 6-10 part, China tree 6-10 part, cockscomb 6-10 part, radix ranunculi ternati 6-10 part, Flos Albiziae 4-8 part, butterflybush flower 4-8 part, salvia chinensis 2-6 part, reed Ba Zi 2-6 part, Golden Larch Bark 2-6 part.
2. a kind of botanical pesticide according to claim 1 and production method, is characterized in that this botanical pesticide is made up of the herbal raw material of following weight portion: 14 parts, Radix Glycyrrhizae, phellodendron bark 8 parts, China tree 8 parts, cockscomb 8 parts, radix ranunculi ternati 8 parts, Flos Albiziae 6 parts, butterflybush flower 6 parts, salvia chinensis 4 parts, reed Ba Zi 4 parts, Golden Larch Bark 4 parts.
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Cited By (9)
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| CN106069563A (en) * | 2016-06-17 | 2016-11-09 | 枞阳县白云生态园林有限责任公司 | A kind of implantation methods of high malaga |
| CN106135303A (en) * | 2016-08-11 | 2016-11-23 | 重庆市井场果蔬专业合作社 | A kind of composition for preventing and treating pear scab |
| CN106212576A (en) * | 2016-07-27 | 2016-12-14 | 广西驰胜农业科技有限公司 | The pesticide control of a kind of Fructus Mangifera Indicae abnormality disease and preparation method |
| CN106259499A (en) * | 2016-08-18 | 2017-01-04 | 梁建 | A kind of compositions preventing and treating sugarcane toppers maize ear rot |
| CN106259541A (en) * | 2016-08-18 | 2017-01-04 | 梁建 | A kind of compositions preventing and treating Caulis Sacchari sinensis brown streak |
| CN106305802A (en) * | 2016-08-19 | 2017-01-11 | 北海市伟恒生态农业有限公司 | Fertilizer for preventing banana mosaic heart rot |
| CN107410382A (en) * | 2017-09-13 | 2017-12-01 | 湖南省农业生物资源利用研究所 | A kind of bactericide for tobacco black shank and its preparation method and application |
| CN107853334A (en) * | 2017-12-14 | 2018-03-30 | 柳州市汉象教育咨询有限责任公司 | A kind of radix ranunculi ternati vegetable insecticide and preparation method thereof |
| CN112400912A (en) * | 2020-12-03 | 2021-02-26 | 贵州省烟草科学研究院 | Chinese herbal medicine for preventing and treating tobacco rhizome diseases and preparation method and application method thereof |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106069563A (en) * | 2016-06-17 | 2016-11-09 | 枞阳县白云生态园林有限责任公司 | A kind of implantation methods of high malaga |
| CN106212576A (en) * | 2016-07-27 | 2016-12-14 | 广西驰胜农业科技有限公司 | The pesticide control of a kind of Fructus Mangifera Indicae abnormality disease and preparation method |
| CN106135303A (en) * | 2016-08-11 | 2016-11-23 | 重庆市井场果蔬专业合作社 | A kind of composition for preventing and treating pear scab |
| CN106259499A (en) * | 2016-08-18 | 2017-01-04 | 梁建 | A kind of compositions preventing and treating sugarcane toppers maize ear rot |
| CN106259541A (en) * | 2016-08-18 | 2017-01-04 | 梁建 | A kind of compositions preventing and treating Caulis Sacchari sinensis brown streak |
| CN106305802A (en) * | 2016-08-19 | 2017-01-11 | 北海市伟恒生态农业有限公司 | Fertilizer for preventing banana mosaic heart rot |
| CN107410382A (en) * | 2017-09-13 | 2017-12-01 | 湖南省农业生物资源利用研究所 | A kind of bactericide for tobacco black shank and its preparation method and application |
| CN107410382B (en) * | 2017-09-13 | 2020-04-14 | 湖南省农业生物资源利用研究所 | Bactericide for tobacco black shank and preparation method and application thereof |
| CN107853334A (en) * | 2017-12-14 | 2018-03-30 | 柳州市汉象教育咨询有限责任公司 | A kind of radix ranunculi ternati vegetable insecticide and preparation method thereof |
| CN112400912A (en) * | 2020-12-03 | 2021-02-26 | 贵州省烟草科学研究院 | Chinese herbal medicine for preventing and treating tobacco rhizome diseases and preparation method and application method thereof |
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