CN104911275A - Bacterial vaginitis detection kit - Google Patents
Bacterial vaginitis detection kit Download PDFInfo
- Publication number
- CN104911275A CN104911275A CN201510409316.0A CN201510409316A CN104911275A CN 104911275 A CN104911275 A CN 104911275A CN 201510409316 A CN201510409316 A CN 201510409316A CN 104911275 A CN104911275 A CN 104911275A
- Authority
- CN
- China
- Prior art keywords
- primer
- seq
- bacterial vaginitis
- detection kit
- lamp
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000001514 detection method Methods 0.000 title claims abstract description 28
- 208000004926 Bacterial Vaginosis Diseases 0.000 title claims abstract description 24
- 239000011535 reaction buffer Substances 0.000 claims abstract description 12
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 11
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 11
- 150000007523 nucleic acids Chemical class 0.000 claims abstract description 11
- 239000000980 acid dye Substances 0.000 claims abstract description 7
- 239000002773 nucleotide Substances 0.000 claims description 8
- 125000003729 nucleotide group Chemical group 0.000 claims description 8
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 claims description 6
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 claims description 6
- 239000003153 chemical reaction reagent Substances 0.000 claims description 6
- CGNLCCVKSWNSDG-UHFFFAOYSA-N SYBR Green I Chemical compound CN(C)CCCN(CCC)C1=CC(C=C2N(C3=CC=CC=C3S2)C)=C2C=CC=CC2=[N+]1C1=CC=CC=C1 CGNLCCVKSWNSDG-UHFFFAOYSA-N 0.000 claims description 5
- 239000013642 negative control Substances 0.000 claims description 5
- 239000013641 positive control Substances 0.000 claims description 5
- 238000007400 DNA extraction Methods 0.000 claims description 3
- KWIUHFFTVRNATP-UHFFFAOYSA-N glycine betaine Chemical compound C[N+](C)(C)CC([O-])=O KWIUHFFTVRNATP-UHFFFAOYSA-N 0.000 claims description 3
- 238000012360 testing method Methods 0.000 abstract description 8
- 239000000243 solution Substances 0.000 abstract description 5
- 238000007397 LAMP assay Methods 0.000 abstract description 3
- 108020004414 DNA Proteins 0.000 abstract 2
- 102000053602 DNA Human genes 0.000 abstract 1
- 241000193385 Geobacillus stearothermophilus Species 0.000 abstract 1
- 241000207201 Gardnerella vaginalis Species 0.000 description 17
- 238000006243 chemical reaction Methods 0.000 description 16
- 238000000034 method Methods 0.000 description 11
- LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical group [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 description 9
- 238000003199 nucleic acid amplification method Methods 0.000 description 6
- 230000003321 amplification Effects 0.000 description 5
- 239000000284 extract Substances 0.000 description 5
- 239000011536 extraction buffer Substances 0.000 description 5
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 4
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 239000007795 chemical reaction product Substances 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 241000725303 Human immunodeficiency virus Species 0.000 description 3
- 206010046914 Vaginal infection Diseases 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 230000035945 sensitivity Effects 0.000 description 3
- 108050009160 DNA polymerase 1 Proteins 0.000 description 2
- 241000207202 Gardnerella Species 0.000 description 2
- 201000008100 Vaginitis Diseases 0.000 description 2
- 208000037009 Vaginitis bacterial Diseases 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 230000001404 mediated effect Effects 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 238000011895 specific detection Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 2
- 208000004145 Endometritis Diseases 0.000 description 1
- 101150092075 FIP1 gene Proteins 0.000 description 1
- 238000003794 Gram staining Methods 0.000 description 1
- 241000283986 Lepus Species 0.000 description 1
- 239000012807 PCR reagent Substances 0.000 description 1
- 241000191992 Peptostreptococcus Species 0.000 description 1
- 208000006399 Premature Obstetric Labor Diseases 0.000 description 1
- 206010036600 Premature labour Diseases 0.000 description 1
- 210000001744 T-lymphocyte Anatomy 0.000 description 1
- 241000588902 Zymomonas mobilis Species 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 238000003149 assay kit Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 210000003756 cervix mucus Anatomy 0.000 description 1
- 208000035850 clinical syndrome Diseases 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000003113 dilution method Methods 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 238000010166 immunofluorescence Methods 0.000 description 1
- 238000011901 isothermal amplification Methods 0.000 description 1
- 238000003771 laboratory diagnosis Methods 0.000 description 1
- 210000002540 macrophage Anatomy 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000005706 microflora Species 0.000 description 1
- 210000001616 monocyte Anatomy 0.000 description 1
- 208000026440 premature labor Diseases 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 238000004153 renaturation Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 239000013076 target substance Substances 0.000 description 1
- 210000001215 vagina Anatomy 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
- 238000012800 visualization Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
Claims (5)
Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201610017748.1A CN106350581B (en) | 2015-07-13 | 2015-07-13 | Method for detecting bacterial vaginitis by using detection kit |
CN201510601653.XA CN105087812B (en) | 2015-07-13 | 2015-07-13 | A kind of bacterial vaginitis detection kit |
CN201510409316.0A CN104911275B (en) | 2015-07-13 | 2015-07-13 | A kind of bacterial vaginitis detection kit |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201510409316.0A CN104911275B (en) | 2015-07-13 | 2015-07-13 | A kind of bacterial vaginitis detection kit |
Related Child Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201610017748.1A Division CN106350581B (en) | 2015-07-13 | 2015-07-13 | Method for detecting bacterial vaginitis by using detection kit |
CN201510601653.XA Division CN105087812B (en) | 2015-07-13 | 2015-07-13 | A kind of bacterial vaginitis detection kit |
Publications (2)
Publication Number | Publication Date |
---|---|
CN104911275A true CN104911275A (en) | 2015-09-16 |
CN104911275B CN104911275B (en) | 2016-02-17 |
Family
ID=54080715
Family Applications (3)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201510601653.XA Active CN105087812B (en) | 2015-07-13 | 2015-07-13 | A kind of bacterial vaginitis detection kit |
CN201610017748.1A Active CN106350581B (en) | 2015-07-13 | 2015-07-13 | Method for detecting bacterial vaginitis by using detection kit |
CN201510409316.0A Active CN104911275B (en) | 2015-07-13 | 2015-07-13 | A kind of bacterial vaginitis detection kit |
Family Applications Before (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201510601653.XA Active CN105087812B (en) | 2015-07-13 | 2015-07-13 | A kind of bacterial vaginitis detection kit |
CN201610017748.1A Active CN106350581B (en) | 2015-07-13 | 2015-07-13 | Method for detecting bacterial vaginitis by using detection kit |
Country Status (1)
Country | Link |
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CN (3) | CN105087812B (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2021534764A (en) * | 2018-08-24 | 2021-12-16 | ジェン−プローブ・インコーポレーテッド | Compositions and Methods for Detecting Bacterial Nucleic Acids and Diagnosing Bacterial Vaginal Inflammation |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105087812B (en) * | 2015-07-13 | 2016-10-12 | 北京博奥医学检验所有限公司 | A kind of bacterial vaginitis detection kit |
CN105420360A (en) * | 2015-12-09 | 2016-03-23 | 广州和实生物技术有限公司 | Kit for instantly detecting vaginitis pathogen gene |
US20210172005A1 (en) * | 2017-11-24 | 2021-06-10 | The University Of Western Australia | Infection-related preterm birth diagnostic method |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20120264126A1 (en) * | 2009-12-03 | 2012-10-18 | Quest Diagnostics Investments Incorporated | Methods for the diagnosis of bacterial vaginosis |
CN103333961A (en) * | 2013-06-19 | 2013-10-02 | 兰州百源基因技术有限公司 | Primer, probe and method for detecting gardnerella vaginalis and kit |
CN103882134A (en) * | 2014-03-31 | 2014-06-25 | 深圳意达凯生物科技有限公司 | Primer pair for detecting gardnerella vaginalis, kit and application thereof |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1493698A (en) * | 2002-07-02 | 2004-05-05 | 昆明寰基生物芯片开发有限公司 | Determination of one section vagina bacillus Gardner DNA sequence (3) specificity and its application |
CN1495265A (en) * | 2002-07-02 | 2004-05-12 | 昆明寰基生物芯片开发有限公司 | Definition of Gardner's bacillus DNA sequence (1) specificity of vagina and its application |
CN1493699A (en) * | 2002-07-02 | 2004-05-05 | 昆明寰基生物芯片开发有限公司 | Determination of one section vagina bucillus Gardner DNA sequence (2) specificity and its application |
CN104328212B (en) * | 2014-11-24 | 2016-08-17 | 济南市中心医院 | The primer sets of ring mediated isothermal amplification method detection B group streptococcus and test kit |
CN105087812B (en) * | 2015-07-13 | 2016-10-12 | 北京博奥医学检验所有限公司 | A kind of bacterial vaginitis detection kit |
-
2015
- 2015-07-13 CN CN201510601653.XA patent/CN105087812B/en active Active
- 2015-07-13 CN CN201610017748.1A patent/CN106350581B/en active Active
- 2015-07-13 CN CN201510409316.0A patent/CN104911275B/en active Active
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US20120264126A1 (en) * | 2009-12-03 | 2012-10-18 | Quest Diagnostics Investments Incorporated | Methods for the diagnosis of bacterial vaginosis |
CN103333961A (en) * | 2013-06-19 | 2013-10-02 | 兰州百源基因技术有限公司 | Primer, probe and method for detecting gardnerella vaginalis and kit |
CN103882134A (en) * | 2014-03-31 | 2014-06-25 | 深圳意达凯生物科技有限公司 | Primer pair for detecting gardnerella vaginalis, kit and application thereof |
Non-Patent Citations (2)
Title |
---|
蓝玉清等人: "直接免疫荧光法在阴道加德纳菌感染诊断中的应用研究", 《国际检验医学杂志》 * |
韩红玉等人: "阴道加德纳菌套式PCR 检测及其临床应用", 《中国优生与遗传杂志》 * |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2021534764A (en) * | 2018-08-24 | 2021-12-16 | ジェン−プローブ・インコーポレーテッド | Compositions and Methods for Detecting Bacterial Nucleic Acids and Diagnosing Bacterial Vaginal Inflammation |
JP7389111B2 (en) | 2018-08-24 | 2023-11-29 | ジェン-プローブ・インコーポレーテッド | Compositions and methods for detecting bacterial nucleic acids and diagnosing bacterial vaginosis |
Also Published As
Publication number | Publication date |
---|---|
CN105087812A (en) | 2015-11-25 |
CN105087812B (en) | 2016-10-12 |
CN104911275B (en) | 2016-02-17 |
CN106350581B (en) | 2020-09-18 |
CN106350581A (en) | 2017-01-25 |
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Legal Events
Date | Code | Title | Description |
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C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
CB02 | Change of applicant information |
Address after: 264200 No. 6, ring mountain road, Weihai economic and Technological Development Zone, Shandong Applicant after: Xu Jing Address before: 210000 No. 18, Hankou Road, Gulou District, Jiangsu, Nanjing Applicant before: Xu Jing |
|
COR | Change of bibliographic data | ||
C41 | Transfer of patent application or patent right or utility model | ||
CB03 | Change of inventor or designer information |
Inventor after: Ge Tao Inventor after: Zhang Yinling Inventor after: Zhang Lei Inventor before: Xu Jing |
|
COR | Change of bibliographic data | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20160120 Address after: 102206 Beijing City, Changping District small town Zhongguancun Life Science Park No. 8, 1 District 1 Building 1 room 116 (Changping Park) Applicant after: BEIJING YIYAN PARTNER MEDICINEINSTITUTE CO.,LTD. Address before: 264200 No. 6, ring mountain road, Weihai economic and Technological Development Zone, Shandong Applicant before: Xu Jing |
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C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
CP03 | Change of name, title or address |
Address after: 211800 12 / F, Kechuang No.1 building, NO.320, pubin Road, Jiangpu street, Pukou District, Nanjing City, Jiangsu Province Patentee after: Nanjing gene Biotechnology Co.,Ltd. Address before: 102206 Beijing City, Changping District small town Zhongguancun Life Science Park No. 8, 1 District 1 Building 1 room 116 (Changping Park) Patentee before: BEIJING YIYAN PARTNER MEDICINEINSTITUTE CO.,LTD. |
|
CP03 | Change of name, title or address | ||
CP03 | Change of name, title or address | ||
CP03 | Change of name, title or address |
Address after: Room A479, 4 / F, 101-8 / F, building 4, yard 3, Fuxing 4th Street, Shunyi District, Beijing Patentee after: Beijing jiyinbofu Biotechnology Co.,Ltd. Address before: 211800 12 / F, Kechuang No.1 building, NO.320, pubin Road, Jiangpu street, Pukou District, Nanjing City, Jiangsu Province Patentee before: Nanjing gene Biotechnology Co.,Ltd. |