CN104906200A - Bitter buckwheat bran total flavonoid preparing method and application of same in decreasing blood pressure - Google Patents
Bitter buckwheat bran total flavonoid preparing method and application of same in decreasing blood pressure Download PDFInfo
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- CN104906200A CN104906200A CN201510270793.3A CN201510270793A CN104906200A CN 104906200 A CN104906200 A CN 104906200A CN 201510270793 A CN201510270793 A CN 201510270793A CN 104906200 A CN104906200 A CN 104906200A
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- Prior art keywords
- radix
- bran
- bitter buckwheat
- rhizoma fagopyri
- fagopyri tatarici
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Abstract
The invention relates to a bitter buckwheat bran total flavonoid preparing method and an application of the same in decreasing blood pressure. The bitter buckwheat bran total flavonoid preparing method includes the following steps of placing smashed bitter buckwheat bran in an ultrasonic-microwave-assisted extraction device, adding a 50-60% ethanol solution three to five times of the smashed bitter buckwheat bran, extracting the mixture for 2-3 hours on the conditions of normally-open 500W of ultrasonic waves and 400-600W of microwave power, filtering the mixture until the filtrate is concentrated to be of a size one fifth to one third of the original size, overlaying macroporous adsorption resin with the concentrated liquor, conducting ethyl alcohol elution, collecting eluant and drying the eluant to constant weight. The prepared bitter buckwheat bran total flavonoids have a remarkable blood pressure reduction effect and have the wide application prospects.
Description
Technical field
The invention belongs to medical art, be specifically related to a kind of preparation method and blood pressure lowering application thereof of bran of Radix Et Rhizoma Fagopyri Tatarici total flavones.
Background technology
Radix Et Rhizoma Fagopyri Tatarici is integration of edible and medicinal herbs food, and traditional medicine and modern medicine all confirm that Radix Et Rhizoma Fagopyri Tatarici has the effects such as blood sugar lowering, blood fat reducing, urine glucose lowering, anti-constipation, are called " three fall food " and " health food of the 21 century mankind " by authoritative expert.Radix Et Rhizoma Fagopyri Tatarici is not only nutritious, and also containing a large amount of flavone compounds, its main component is rutin, and content accounts for 70 ~ 90% of general flavone content.Bioflavonoids is the topmost bioactive ingredients of Radix Et Rhizoma Fagopyri Tatarici, and various pharmacological action and its of Radix Et Rhizoma Fagopyri Tatarici contain abundant bioflavonoids and be undivided.
Flavone compound is the secondary metabolites be extensively present in plant, is one of main active in medicinal plants, the multiformity of its structure type and bioactive broad spectrum activity, is the focus of research and development both at home and abroad in recent years always.Existing research shows that it has significant antioxidation, removes the medicinal health functions such as human free radical, defying age, mutation, blood fat reducing, blood pressure lowering, and due to efficient, safety, also can be used as the natural additive of food, cosmetics, there is wide DEVELOPMENT PROSPECT.
Hypertension a kind ofly continues to raise as the chronic disease of main manifestations with arteriotony, and often cause the pathological changes of the vitals such as the heart, brain, kidney and occur corresponding consequence, hypertension belongs to " headache ", " dizzy " category of the traditional Chinese medical science.The blood pressure standard that World Health Organization's suggestion uses is: adult normal's systolic pressure should be less than or equal to 140mmHg, and diastolic pressure is less than or equal to 90 mmHg; Systolic pressure is between 141 ~ 159 mmHg, and diastolic pressure, between 91 ~ 94mmHg, is borderline hypertension.
And present stage, the resisting hypertension medicine price that is suitable for more expensive, especially Western medicine easily causes obvious drug-resistant effect, causes patient more and more stronger to the dependency of medicine, and the dose of medicine is also more and more higher.
Summary of the invention
The object of the present invention is to provide a kind of preparation method and application thereof of bran of Radix Et Rhizoma Fagopyri Tatarici total flavones, this total flavones has the effect of the blood pressure lowering of highly significant.
For achieving the above object, the present invention adopts following technical scheme:
Bran of Radix Et Rhizoma Fagopyri Tatarici after pulverizing is placed in ultrasonic-microwave extracting apparatus, add the alcoholic solution of 50 ~ 60% of raw material 3 ~ 5 times, often open in ultrasound wave 500W, microwave power is extraction 2 ~ 3h under the condition of 400 ~ 600W, filtration, filtrate is concentrated into 1/5 ~ 1/3 of original volume, macroporous adsorbent resin on gained concentrated solution, first uses the distilled water eluting of 2 ~ 3BV, rear use 5 ~ 7BV 60 ~ 70% ethanol elution, collect eluent, concentrating under reduced pressure, is dried to constant weight, obtains bran of Radix Et Rhizoma Fagopyri Tatarici total flavones.
Described macroporous adsorbent resin type is any one in AB-8, HPD-100, XDA-1.
Bran of Radix Et Rhizoma Fagopyri Tatarici total flavones prepared by the present invention is to the research of rat hypotensive activity:
By spontaneous hypertensive rat in 10 ~ 13 week age (SHR) by blood pressure grouping, concrete dosage is in table 1, and oral administration is arteria caudalis pressure measurement after 7 days.
Statistical analysis: measurement data with mean+SD (
± SD) represent, between two groups, data compare t inspection between employing group.Be significance standard with P < 0.05.
Result: bran of Radix Et Rhizoma Fagopyri Tatarici total flavones can effectively reduce SHR rat blood pressure, compares with model comparison that there were significant differences (P < 0.05), in table 1.
Table 1 bran of Radix Et Rhizoma Fagopyri Tatarici total flavones successive administration is on the impact of SHR rat blood pressure
Compare with model control group
△p < 0.05.
Preparation method of the present invention is simple, with low cost.Experiment proves that the bran of Radix Et Rhizoma Fagopyri Tatarici total flavones of gained has and relaxes the lasting effect of reducing blood pressure, and controlling the systolic pressure of spontaneous hypertensive rat (SHR) preferably, opened up the purposes that bran of Radix Et Rhizoma Fagopyri Tatarici total flavones is new, is the innovation on Chinese medicine.
Further illustrate the present invention below in conjunction with detailed description of the invention, but the scope of protection of present invention is not limited to following embodiments.
detailed description of the invention:
Embodiment 1:
Bran of Radix Et Rhizoma Fagopyri Tatarici after pulverizing is placed in ultrasonic-microwave extracting apparatus, add the alcoholic solution of 50% of raw material 3 times, often open in ultrasound wave 500W, microwave power is extract 2h under the condition of 400W, filter, filtrate is concentrated into 1/3 of original volume, AB-8 macroporous adsorbent resin on gained concentrated solution, first use the distilled water eluting of 2BV, rear 5BV 60% ethanol elution, collect eluent, concentrating under reduced pressure, be dried to constant weight, obtain bran of Radix Et Rhizoma Fagopyri Tatarici total flavones, its yield is 5.18%, detect through UV, the content of its total flavones is 75.34%.
Embodiment 2:
Bran of Radix Et Rhizoma Fagopyri Tatarici after pulverizing is placed in ultrasonic-microwave extracting apparatus, add the alcoholic solution of 60% of raw material 5 times, often open in ultrasound wave 500W, microwave power is extract 3h under the condition of 600W, filter, filtrate is concentrated into 1/5 of original volume, XDA-1 macroporous adsorbent resin on gained concentrated solution, first use the distilled water eluting of 3BV, rear 6BV 65% ethanol elution, collect eluent, concentrating under reduced pressure, be dried to constant weight, obtain bran of Radix Et Rhizoma Fagopyri Tatarici total flavones, its yield is 5.69%, detect through UV, the content of its total flavones is 81.24%.
Embodiment 3:
Bran of Radix Et Rhizoma Fagopyri Tatarici after pulverizing is placed in ultrasonic-microwave extracting apparatus, add the alcoholic solution of 55% of raw material 4 times, often open in ultrasound wave 500W, microwave power is extract 2.5h under the condition of 500W, filter, filtrate is concentrated into 1/4 of original volume, HPD-100 macroporous adsorbent resin on gained concentrated solution, first use the distilled water eluting of 3BV, rear 7BV 70% ethanol elution, collect eluent, concentrating under reduced pressure, be dried to constant weight, obtain bran of Radix Et Rhizoma Fagopyri Tatarici total flavones, its yield is 5.44%, detect through UV, the content of its total flavones is 73.11%.
Claims (3)
1. the preparation method of a bran of Radix Et Rhizoma Fagopyri Tatarici total flavones, it is characterized in that, comprise the steps: that the bran of Radix Et Rhizoma Fagopyri Tatarici after by pulverizing is placed in ultrasonic-microwave extracting apparatus, add the alcoholic solution of 50 ~ 60% of raw material 3 ~ 5 times, often open in ultrasound wave 500W, microwave power is extract 2 ~ 3h under the condition of 400 ~ 600W, filter, filtrate is concentrated into 1/5 ~ 1/3 of original volume, macroporous adsorbent resin on gained concentrated solution, first use the distilled water eluting of 2 ~ 3BV, use the ethanol elution of 60 ~ 70% of 5 ~ 7BV afterwards, collect eluent, concentrating under reduced pressure, be dried to constant weight, obtain bran of Radix Et Rhizoma Fagopyri Tatarici total flavones.
2. the preparation method of a kind of bran of Radix Et Rhizoma Fagopyri Tatarici total flavones according to claim 1, is characterized in that, described macroporous adsorbent resin type is any one in AB-8, HPD-100, XDA-1.
3. bran of Radix Et Rhizoma Fagopyri Tatarici total flavones according to claim 1 is preparing the application had in the medicine of hypotensive activity.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510270793.3A CN104906200A (en) | 2015-05-26 | 2015-05-26 | Bitter buckwheat bran total flavonoid preparing method and application of same in decreasing blood pressure |
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| CN201510270793.3A CN104906200A (en) | 2015-05-26 | 2015-05-26 | Bitter buckwheat bran total flavonoid preparing method and application of same in decreasing blood pressure |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105920114A (en) * | 2016-04-18 | 2016-09-07 | 浙江大学 | Black tartary buckwheat rice extract with blood sugar decreasing function and preparation method thereof |
| CN110787220A (en) * | 2019-12-11 | 2020-02-14 | 中国科学院成都生物研究所 | Method for extracting and purifying total flavonoids of tartary buckwheat bran |
| CN114152690A (en) * | 2021-11-25 | 2022-03-08 | 重庆长安汽车股份有限公司 | Method for detecting acetaldehyde content in solid material |
-
2015
- 2015-05-26 CN CN201510270793.3A patent/CN104906200A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105920114A (en) * | 2016-04-18 | 2016-09-07 | 浙江大学 | Black tartary buckwheat rice extract with blood sugar decreasing function and preparation method thereof |
| CN110787220A (en) * | 2019-12-11 | 2020-02-14 | 中国科学院成都生物研究所 | Method for extracting and purifying total flavonoids of tartary buckwheat bran |
| CN114152690A (en) * | 2021-11-25 | 2022-03-08 | 重庆长安汽车股份有限公司 | Method for detecting acetaldehyde content in solid material |
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Application publication date: 20150916 |