CN106038944A - A method of preparing allium mongolicum total flavonoids and applications of the total flavonoids - Google Patents
A method of preparing allium mongolicum total flavonoids and applications of the total flavonoids Download PDFInfo
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- CN106038944A CN106038944A CN201610306946.XA CN201610306946A CN106038944A CN 106038944 A CN106038944 A CN 106038944A CN 201610306946 A CN201610306946 A CN 201610306946A CN 106038944 A CN106038944 A CN 106038944A
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- goldball onion
- total flavones
- total flavonoids
- goldball
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
- A61K36/8962—Allium, e.g. garden onion, leek, garlic or chives
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
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Abstract
A method of preparing allium mongolicum total flavonoids and applications of the total flavonoids are disclosed. The method includes (1) drying allium mongolicum in the shade and crushing, (2) extracting with ultrasonic waves, (3) purifying with polyamide resin, eluting with ethanol and collecting an eluate, and (4) concentrating and drying to obtain the total flavonoids. Animal experiments prove that the total flavonoids have good functions of lowering the blood pressure and can be widely used for preparing medicines reducing the blood pressure.
Description
Technical field
Present invention relates particularly to the preparation method and applications of a kind of goldball onion total flavones.
Background technology
Flavone compound is the secondary metabolites being widely present in plant, is that in medicinal plants, chief active becomes
/ mono-, the multiformity of its structure type and bioactive broad spectrum activity, the in recent years focus of research and development the most both at home and abroad.
Existing research shows that it has significant antioxidation, removes human free radical, defying age, mutation, blood fat reducing, blood pressure lowering etc.
Medicinal health function, and due to efficient, safety, also act as the natural additive of food, cosmetics, before there is wide exploitation
Scape.
At present, the research to goldball onion total flavones is concentrated mainly in its chemical composition, and less to its pharmacological research.High blood
Pressure is a kind of chronic disease persistently rising a height of main performance with arteriotony, often causes the pathological changes of the vitals such as the heart, brain, kidney
And corresponding consequence occurs, hypertension belongs to " headache ", " dizziness " category of the traditional Chinese medical science.The blood pressure mark that World Health Organization's suggestion uses
Standard is: adult normal shrinks pressure and should be less than or be equal to 140mmHg, and diastolic pressure is less than or equal to 90 mmHg；Contraction is pressed in 141 ~
Between 159 mmHg, diastolic pressure is between 91 ~ 94mmHg, for borderline hypertension.
And present stage, the resisting hypertension medicine being suitable for is expensive, and especially Western medicine easily causes obvious drug resistance to make
With, cause patient increasingly stronger to the dependency of medicine, the dose of medicine is more and more higher.
The Mongolian Herba Alii fistulosi of goldball onion another name, formal name used at school Allium mongolicum Regel, Mongolian entitled Hu Muli, belong to allium, Liliaceae, angiosperm
Door, is a kind of typical wild vegetable, has the pungent of Herba Alii fistulosi, the unique fragrance that also family Herba Alii fistulosi does not has, and herdsman is commonly used for seasoning
Product and vegetable.The nutrition of goldball onion is the abundantest, and containing the multivitamin of needed by human body, and it is grown in again without any pollution
On grassland, desert, it therefore it is perfect natural green food.
Summary of the invention
It is an object of the invention to provide the preparation method and applications of a kind of goldball onion total flavones, this goldball onion total flavones has
The effect of the blood pressure lowering of highly significant.
For achieving the above object, the present invention adopts the following technical scheme that
(1) goldball onion dried in the shade, pulverize；
(2) goldball onion powder is placed in ultrosonic extractor, the ethanol solution of the 70 ~ 80% of addition raw material weight 6 ~ 8 times, if
Putting ultrasonic temperature is 60 ~ 70 DEG C, and the time is 2 ~ 3h, filters, and filtering residue repeats to extract 2 times, merging filtrate；
(3) filtrate of gained in step (2) is concentrated into without alcohol taste, proceeds to the polyamide resin column that blade diameter length ratio is 1:4 ~ 6, first
With the distilled water eluting of 2 ~ 3 times of column volumes, afterwards with the 70 ~ 80% of 4 ~ 6 times of column volumes ethanol elution, collect eluent；
(4) eluent concentrates, and is dried, obtains goldball onion total flavones.
The frequency of the ultrasonic unit described in step (1) is 20KHz.
The mesh number of the polyamide described in step (3) is 80 mesh.
The gained goldball onion total flavones of the present invention research to rat hypotensive activity:
By blood pressure, 10 ~ 13 week old spontaneous hypertensive rats (SHR) being divided into 4 groups, often group 10, concrete dosage is shown in Table 1,
Oral administration arteria caudalis pressure measurement after 7 days.
Statistical analysis: measurement data mean+SD (x ± s) represents, between two groups, data compare t inspection between employing group
Test.With P ＜ 0.05 for significance standard.Result such as table 1:
The impact on SHR rat blood pressure of the table 1 goldball onion total flavones successive administration
Compare with model control group△P ＜ 0.05.
Result shows, goldball onion total flavones basic, normal, high dosage group all can be effectively reduced SHR rat blood pressure, and in certain
Dose dependent.
The beneficial effects of the present invention is: technique is simple, low cost, meet environmental protection and use requirement；Long shelf-life, can be wide
The general treatment and prevention for diseases such as blood pressure lowering.
Further illustrate the present invention below in conjunction with detailed description of the invention, but the scope of protection of present invention is not limited to
In following embodiments.
Detailed description of the invention:
Embodiment 1:
Goldball onion is dried in the shade, pulverizes；Goldball onion powder is placed in the ultrosonic extractor that frequency is 20KHz, adds raw material weight 6
The ethanol solution of 70% again, arranging ultrasonic temperature is 60 DEG C, and the time is 2h, filters, and filtering residue repeats to extract 2 times, merging filtrate；
The filtrate of gained is concentrated into without alcohol taste, proceeds to the polyamide resin column of 80 mesh that blade diameter length ratio is 1:4, first with 2 times of column volumes
Distilled water eluting, rear with the 70% of 4 times of column volumes ethanol elution, collection eluent；Eluent concentrates, and is dried, obtains goldball onion total
Flavone, detects through UV, and the content of its total flavones is 63.4%.
Embodiment 2:
Goldball onion is dried in the shade, pulverizes；Goldball onion powder is placed in the ultrosonic extractor that frequency is 20KHz, adds raw material weight 7
The ethanol solution of 75% again, arranging ultrasonic temperature is 70 DEG C, and the time is 3h, filters, and filtering residue repeats to extract 2 times, merging filtrate；
The filtrate of gained is concentrated into without alcohol taste, proceeds to the polyamide resin column of 80 mesh that blade diameter length ratio is 1:6, first with 3 times of column volumes
Distilled water eluting, rear with the 75% of 5 times of column volumes ethanol elution, collection eluent；Eluent concentrates, and is dried, obtains goldball onion total
Flavone, detects through UV, and the content of its total flavones is 77.8%.
Embodiment 3:
Goldball onion is dried in the shade, pulverizes；Goldball onion powder is placed in the ultrosonic extractor that frequency is 20KHz, adds raw material weight 8
The ethanol solution of 80% again, arranging ultrasonic temperature is 65 DEG C, and the time is 3h, filters, and filtering residue repeats to extract 2 times, merging filtrate；
The filtrate of gained is concentrated into without alcohol taste, proceeds to the polyamide resin column of 80 mesh that blade diameter length ratio is 1:5, first with 3 times of column volumes
Distilled water eluting, rear with the 80% of 6 times of column volumes ethanol elution, collection eluent；Eluent concentrates, and is dried, obtains goldball onion total
Flavone, detects through UV, and the content of its total flavones is 59.6%.
Claims (4)
1. the preparation method of a goldball onion total flavones, it is characterised in that comprise the following steps:
(1) goldball onion dried in the shade, pulverize；
(2) goldball onion powder is placed in ultrosonic extractor, the ethanol solution of the 70 ~ 80% of addition raw material weight 6 ~ 8 times, if
Putting ultrasonic temperature is 60 ~ 70 DEG C, and the time is 2 ~ 3h, filters, and filtering residue repeats to extract 2 times, merging filtrate；
(3) filtrate of gained in step (2) is concentrated into without alcohol taste, proceeds to the polyamide resin column that blade diameter length ratio is 1:4 ~ 6, first use
The distilled water eluting of 2 ~ 3 times of column volumes, afterwards with the 70 ~ 80% of 4 ~ 6 times of column volumes ethanol elution, collect eluent；
(4) eluent concentrates, and is dried, obtains goldball onion total flavones.
The preparation method of a kind of goldball onion total flavones the most according to claim 1, it is characterised in that described in step (1)
The frequency of ultrasonic unit is 20KHz.
The preparation method of a kind of goldball onion total flavones the most according to claim 1, it is characterised in that described in step (3)
The mesh number of polyamide is 80 mesh.
The goldball onion total flavones that the most according to claim 1, prepared by method application in preparing Altace Ramipril.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201610306946.XA CN106038944A (en) | 2016-05-11 | 2016-05-11 | A method of preparing allium mongolicum total flavonoids and applications of the total flavonoids |
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| CN201610306946.XA CN106038944A (en) | 2016-05-11 | 2016-05-11 | A method of preparing allium mongolicum total flavonoids and applications of the total flavonoids |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112450349A (en) * | 2020-11-20 | 2021-03-09 | 杭州顶誉氧科生物科技有限公司 | Solid beverage for supplementing nutrition to body and compacting skin |
| CN114949106A (en) * | 2022-04-18 | 2022-08-30 | 内蒙古农业大学 | Method for extracting allium mongolicum regel flavone |
-
2016
- 2016-05-11 CN CN201610306946.XA patent/CN106038944A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112450349A (en) * | 2020-11-20 | 2021-03-09 | 杭州顶誉氧科生物科技有限公司 | Solid beverage for supplementing nutrition to body and compacting skin |
| CN114949106A (en) * | 2022-04-18 | 2022-08-30 | 内蒙古农业大学 | Method for extracting allium mongolicum regel flavone |
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Application publication date: 20161026 |
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| WD01 | Invention patent application deemed withdrawn after publication |