CN104906174A - Fermented traditional Chinese medicine for preventing and treating infectious bursal disease of chicken and preparation method of fermented traditional Chinese medicine - Google Patents
Fermented traditional Chinese medicine for preventing and treating infectious bursal disease of chicken and preparation method of fermented traditional Chinese medicine Download PDFInfo
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Abstract
The invention discloses a fermented traditional Chinese medicine for preventing and treating an infectious bursal disease of chicken and a preparation method of the fermented traditional Chinese medicine, and belongs to the technical field of veterinary traditional Chinese medicines. The fermented traditional Chinese medicine is prepared by inoculating probiotics into a traditional Chinese medicine extract liquid and a nutrient additive as raw materials, and carrying out anaerobic fermentation, wherein the nutrient additive is prepared from bean pulp, brown sugar and peptone; 3-5g of bean pulp, 3-5g of brown sugar and 1.5-2.5g of peptone are added to each 80-100mL of traditional Chinese medicine extract liquid; and each 1mL of traditional Chinese medicine extract liquid is prepared by decocting and extracting 0.8-1.2g of raw materials. After the traditional Chinese medicine extract liquid is fermented by the probiotics, the activity of effective components is enhanced; the toxicity is reduced; the utilization rate of Chinese herbal medicines is increased; and macromolecular substances which cannot be absorbed in the fermentation process are degraded into micromolecular substances which can be directly absorbed and utilized by the organism, and are matched with active substances generated by fermental cultivation and probiotic metabolites, so that the prevention and treatment effect on the diseases caused by the medicine can be synergistically increased.
Description
Technical field
The present invention relates to a kind of fermented type Chinese medicine, being specifically related to the fermented type Chinese medicine for preventing and treating infectious bursal disease, also relating to the preparation method of this fermented type Chinese medicine simultaneously, belonging to technical field of Chinese medicine for animals.
Background technology
Infectious bursal disease, also known as infectious bursal disease, is a kind of Acute exposure sexually transmitted disease caused by infectious bursa of Fabricius virus.This disease is badly damaged as feature with fabricius bursa inflammation, necrosis, atrophy and fabricius bursa endolymph cell, can cause the immunodeficiency disease of chicken, disturb the immune effect of various vaccine.The sickness rate of infectious bursal disease is high, almost reaches 100%, and mortality rate is generally 5 ~ 15%, sanitary condition difference and occur together other diseases time mortality rate can significantly rise, be one of most important disease of current aviculture.
In veterinary drug allusion quotation, Fuzheng Jiedu powder is mainly used in treating chicken bursal disease, but powder is taken after needing spice, and use very inconvenience, and powder not easily absorbs, prevention effect is undesirable.For this reason, the patent of publication No. CN103830313A makes improvement for conventional dosage forms, provide a kind of oral liquid for strengthening healthy energy and relieving internal heat or fever for animals, with Radix Isatidis, the Radix Astragali, Herba Epimedii for crude drug, traditional decocting method is adopted to extract effective ingredient in crude drug, extracting solution obtains concentrated solution through concentrating under reduced pressure, then adds in concentrated solution by sodium benzoate, tween 80, and the standardize solution that adds water obtains.This oral liquid stable in properties, drug effect higher than traditional powder, and is added by water way, easy to use.But, adopt traditional water decoction method to extract lower to the utilization rate of crude drug, and in medicinal liquid, macromolecular substances is difficult to be absorbed and used, and does not give prominence to the prevention effect of infectious bursal disease.
Summary of the invention
The object of this invention is to provide a kind of fermented type Chinese medicine effectively preventing and treating infectious bursal disease, to improve crude drug utilization rate, increase the content easily absorbing small-molecule active substance in medicine, improve the prevention effect of medicine to infectious bursal disease.
Meanwhile, the present invention also provides a kind of preparation method of above-mentioned fermented type Chinese medicine.
In order to realize above object, the technical solution adopted in the present invention is:
Fermented type Chinese medicine, with Chinese medicine extraction liquid, nutritional supplements for raw material, obtain through anaerobic fermentation after inoculation probiotic bacteria, described nutritional supplements is made up of bean cake, brown sugar and peptone, adds bean cake 3 ~ 5g, brown sugar 3 ~ 5g, peptone 1.5 ~ 2.5g in every 80 ~ 100mL Chinese medicine extraction liquid; Every 1mL Chinese medicine extraction liquid extracts obtained by 0.8 ~ 1.2g crude drug through decocting, crude drug is made up of Radix Isatidis, the Radix Astragali and Herba Epimedii.
Described probiotic bacteria is lactobacillus, can select any one or multiple combination in the conventional lactobacilluss such as streptococcus thermophilus, Lactobacillus plantarum, enterococcus faecalis.
Described probiotic bacteria is made up of lactobacillus and yeast, as being made up of streptococcus thermophilus, Lactobacillus plantarum, enterococcus faecalis and yeast, streptococcus thermophilus, Lactobacillus plantarum, enterococcus faecalis, saccharomycetic number ratio are (1 ~ 3): (1 ~ 3): (1 ~ 3): (1 ~ 3).Wherein, streptococcus thermophilus, Lactobacillus plantarum, enterococcus faecalis are lactobacillus, yeast is fungus, by this several probiotics Combined culture, the kind of the metabolite in fermentation liquid as lactic acid ferment, extracellular polysaccharide, enzyme and vitamin can be made to compare the more comprehensive of single probiotic's culture, meanwhile, what utilize multiple beneficial bacterium that Chinese medicine extraction liquid can be made to ferment is more abundant, improves fermented type Chinese medicine to the prevention effect of disease.
In described crude drug, the mass ratio of Radix Isatidis, the Radix Astragali, Herba Epimedii is the 2:2:1 prescription of Fuzheng Jiedu powder (in the veterinary drug allusion quotation), also can finely tune on the basis of this proportioning, fine setting is this area routine techniques, and it is substantially identical with the prevention effect of former side.Confect mechanism is Radix Isatidis heat-clearing and toxic substances removing, antiviral, enhancing immunity; Radix Astragali invigorating QI to consolidate the body surface resistance, diuresis detoxification, expelling pus and promoting granulation, enhancing body resistance; Herba Epimedii kidney invigorating and YANG supporting, expelling wind and removing dampness; Probiotic bacteria and metabolite thereof, regulating intestinal canal flora, suppresses pathogenic microorganism, improves immunity of organisms.
Described decocting is extracted as routine techniques, and can refer to the preparation of following method, namely get Radix Isatidis, the Radix Astragali and Herba Epimedii, decocting extracts 2 ~ 3 times, merge extractive liquid, concentrated.As decocting extracts 3 times, in crude drug, namely add the water of 4 ~ 6 times amount (in crude drug quality), boil 25 ~ 40 minutes, filter; In filtering residue, add the water of 2 ~ 4 times amount (in medicinal residues quality) according to upper method respectively, boil 25 ~ 40 minutes; Merge 3 extracting solution, concentrated.
Preferably, add bean cake 4g, brown sugar 4g, peptone 2g in every 90mL Chinese medicine extraction liquid, every 1mL Chinese medicine extraction liquid extracts obtained by 1g crude drug through decocting.
The preferred animal proteinum peptone of described peptone, as beef peptone, Carnis caprae seu ovis peptone etc.
Described anaerobic fermentation is conventional fermentation culture technology, as before fermentation culture by fermentation raw material through autoclaving process (as 121 DEG C of autoclavings 30 minutes), except the component being unsuitable for autoclaving process (as strain, some salt etc.).Under the prerequisite that fermentation raw material, fermented bacterium are certain, can optimize inoculum concentration and the viable count of strain further, fermentation condition (as temperature and time) also can be preferred further.
Preferably, the inoculum concentration of probiotic bacteria is 0.5 ~ 1.5% of raw materials quality (the quality sum of Chinese medicine extraction liquid and nutritional supplements); In probiotic bacteria, total viable count is 5 × 10
7~ 1.5 × 10
8cfu/mL.Preferred, the inoculum concentration of probiotic bacteria is 1%, and total viable count is 1 × 10
8cfu/mL.
Preferably, the condition of anaerobic fermentation is: fermentation temperature 35 ~ 40 DEG C, fermentation time 40 ~ 56 hours.Preferred, fermentation temperature 37 DEG C, fermentation time 48 hours.
Probiotic bacteria can directly be bought, and also can make by oneself.During lactobacillus self-control, solid spawn in picking glycerol cryopreservation tube, in access liquid MRS culture medium, 37 DEG C of anaerobic fermentations 24 hours and get final product.The composition following (g/L) of MRS culture medium: peptone 10.0, beef powder 8.0, yeast powder 4.0, glucose 20.0, dipotassium hydrogen phosphate 2.0, diammonium hydrogen citrate 2.0, sodium acetate 5.0, magnesium sulfate 0.2, manganese sulfate 0.04, Tween 80 1.0, pH value 5.7 ± 0.2,118 DEG C of autoclavings 15 minutes are for subsequent use.
The preparation method of fermented type Chinese medicine is: in Chinese medicine extraction liquid, add nutritional supplements, inoculate probiotic bacteria after autoclaving, and at temperature 35 ~ 40 DEG C, anaerobic fermentation 40 ~ 56 hours, to obtain final product.
Every 1mL Chinese medicine extraction liquid extracts obtained by 0.8 ~ 1.2g crude drug through decocting, crude drug is made up of Radix Isatidis, the Radix Astragali and Herba Epimedii.The mass ratio of Radix Isatidis, the Radix Astragali, Herba Epimedii is the 2:2:1 prescription of Fuzheng Jiedu powder (in the veterinary drug allusion quotation), also can finely tune on the basis of this proportioning.
Concrete, the preparation method of Chinese medicine extraction liquid is: get Radix Isatidis, the Radix Astragali and Herba Epimedii, and decocting extracts 2 ~ 3 times, merge extractive liquid, concentrated, to obtain final product.Decocting extracts can carry out 3 times, as added the water of 4 ~ 6 times amount (in crude drug quality) in crude drug, boils 25 ~ 40 minutes, filters; In filtering residue, add the water of 2 ~ 4 times amount (in medicinal residues quality) according to upper method respectively, boil 25 ~ 40 minutes; Merge 3 extracting solution.
Described nutritional supplements is made up of bean cake, brown sugar and peptone, adds bean cake 3 ~ 5g, brown sugar 3 ~ 5g, peptone 1.5 ~ 2.5g in every 80 ~ 100mL Chinese medicine extraction liquid.
Preferably, add bean cake 4g, brown sugar 4g, peptone 2g in every 90mL Chinese medicine extraction liquid, every 1mL Chinese medicine extraction liquid extracts obtained by 1g crude drug through decocting.
Described probiotic bacteria is lactobacillus, can select any one or multiple combination in the conventional lactobacilluss such as streptococcus thermophilus, Lactobacillus plantarum, enterococcus faecalis.
Described probiotic bacteria is made up of lactobacillus and yeast, as being made up of streptococcus thermophilus, Lactobacillus plantarum, enterococcus faecalis and yeast, streptococcus thermophilus, Lactobacillus plantarum, enterococcus faecalis, saccharomycetic number ratio are (1 ~ 3): (1 ~ 3): (1 ~ 3): (1 ~ 3).
Preferably, the inoculum concentration of probiotic bacteria is 0.5 ~ 1.5% of raw materials quality (the quality sum of Chinese medicine extraction liquid and nutritional supplements); In probiotic bacteria, total viable count is 5 × 10
7~ 1.5 × 10
8cfu/mL.Preferred, the inoculum concentration of probiotic bacteria is 1%, and total viable count is 1 × 10
8cfu/mL.
The condition of described anaerobic fermentation is preferably: fermentation temperature 37 DEG C, fermentation time 48 hours.
Beneficial effect of the present invention:
Probiotic bacteria adds use in feedstuff can produce material impact to growth of animal, body health, can improve intestinal environment, facilitating digestion, suppress pathogen, play health invigorating, control the pathogenetic effect of disease.China's Chinese herbal medicine resource enriches, and some Chinese medicine also has the effect improving and regulate fuselage state, improve immunity of organisms while antibacterial.In the present invention, Radix Isatidis, the growth of the Radix Astragali to probiotic bacteria has facilitation, but the some drugs composition in Chinese medicine extraction liquid is unfavorable for the growth of probiotic bacteria, and Chinese medicine extraction liquid can not provide enough for probiotics fermention, comprehensive nutrition, in view of above-mentioned harmful effect and nutritional deficiency, the present invention adds targetedly by bean cake, the nutritional supplements that brown sugar and peptone (especially animal proteinum peptone) form, improve quantity and the activity of probiotic bacteria on the one hand, improve the utilization rate of nutritional supplements and the utilization rate of crude drug on the other hand, reduce fermentation costs, make probiotic bacteria play Fermentation to Chinese medicine extraction liquid better simultaneously, the macromolecular substances that can not be absorbed and used in medicine is more degraded to the small-molecule substance that can be utilized by body, and, the new active matter mass-energy that probiotic bacteria produces in metabolic process improves the action effect of medicine.
In the present invention, Chinese medicine extraction liquid is after probiotics fermention, and effective ingredient increased activity, toxicity reduce, and Chinese herbal medicine utilization rate raises; In sweat, unabsorbable macromolecular substances is degraded to the small-molecule substance that directly can be absorbed by body, with produce new active substance in sweat and probiotic bacteria metabolite coordinates, can work in coordination with and strengthen medicine prevention of disease and therapeutic effect.This fermented type Chinese medicine is good to the prevention effect of infectious bursal disease, and confirm through 164 routine clinical experiments, effective percentage reaches 93.3%.Under the best circumstances, in tunning, probiotics bacterial number can reach 7.0 × 10
9cfu/g, can regulate chicken intestinal flora, suppresses pathogenic microorganism, improves chicken immunity of organisms, effectively reduces the generation of chicken disease particularly intestinal tract disease, through 43 example tests, disease average attack rate can be made to reduce by 31.8%.
Detailed description of the invention
Following embodiment is only described in further detail the present invention, but does not form any limitation of the invention.
Embodiment 1
Fermented type Chinese medicine in the present embodiment, with Chinese medicine extraction liquid, nutritional supplements for raw material, obtains through anaerobic fermentation after inoculation probiotic bacteria; Chinese medicine extraction liquid extracts obtained by crude drug through decocting, crude drug 90g, is made up of according to mass ratio 2:2:1 Radix Isatidis, the Radix Astragali and Herba Epimedii; Nutritional supplements is made up of 4 grams of bean cake, 4 grams of brown sugar and 2 grams of beef peptones, and probiotic bacteria is single lactobacillus (streptococcus thermophilus), and the inoculum concentration of probiotic bacteria is 1% of raw materials quality, and viable count is 1 × 10
8cfu/mL.
The preparation method of fermented type Chinese medicine, comprises the following steps:
(1) preparation of Chinese medicine extraction liquid: the water first adding 5 times amount (in crude drug quality) in crude drug, boils 30 minutes, filters; In filtering residue, add the water of 2 times amount (in medicinal residues quality) according to upper method respectively, boil 30 minutes; Merge 3 extracting solution, concentrate to obtain 90mL Chinese medicine extraction liquid;
(2) anaerobic fermentation: add nutritional supplements in Chinese medicine extraction liquid, autoclaving 30 minutes at 121 DEG C, inoculating lactic acid bacterium, anaerobic fermentation 48 hours at temperature 37 DEG C, Lactic Acid from Fermentation Broth bacterium bacterium number is 7.0 × 10
9cfu/mL.
Embodiment 2
Fermented type Chinese medicine in the present embodiment, with Chinese medicine extraction liquid, nutritional supplements for raw material, obtains through anaerobic fermentation after inoculation probiotic bacteria; Chinese medicine extraction liquid extracts obtained by crude drug through decocting, crude drug 80g, is made up of according to mass ratio 2:2:1 Radix Isatidis, the Radix Astragali and Herba Epimedii; Nutritional supplements is made up of 3 grams of bean cake, 5 grams of brown sugar and 2 grams of peptones, and probiotic bacteria is single lactobacillus (Lactobacillus plantarum), and the inoculum concentration of probiotic bacteria is 0.5% of raw materials quality, and viable count is 1.5 × 10
8cfu/mL.
The preparation method of fermented type Chinese medicine, comprises the following steps:
(1) preparation of Chinese medicine extraction liquid: the water first adding 4 times amount (in crude drug quality) in crude drug, boils 30 minutes, filters; In filtering residue, add the water of 3 times amount (in medicinal residues quality) according to upper method respectively, boil 30 minutes; Merge 3 extracting solution, concentrate to obtain 100mL Chinese medicine extraction liquid;
(2) anaerobic fermentation: add nutritional supplements in Chinese medicine extraction liquid, autoclaving 30 minutes at 121 DEG C, inoculating lactic acid bacterium, anaerobic fermentation 50 hours at temperature 35 DEG C, Lactic Acid from Fermentation Broth bacterium bacterium number is 6.8 × 10
9cfu/mL.
Embodiment 3
Fermented type Chinese medicine in the present embodiment, with Chinese medicine extraction liquid, nutritional supplements for raw material, obtains through anaerobic fermentation after inoculation probiotic bacteria; Chinese medicine extraction liquid extracts obtained by crude drug through decocting, crude drug 120g, is made up of according to mass ratio 2:2:1 Radix Isatidis, the Radix Astragali and Herba Epimedii; Nutritional supplements is made up of 5 grams of bean cake, 3 grams of brown sugar and 2 grams of beef peptones, probiotic bacteria is for be made up of according to number ratio 3:3:3:1 streptococcus thermophilus, Lactobacillus plantarum, enterococcus faecalis, yeast, the inoculum concentration of probiotic bacteria is 1.5% of raw materials quality, and total viable count is 0.5 × 10
8cfu/mL.
The preparation method of fermented type Chinese medicine, comprises the following steps:
(1) preparation of Chinese medicine extraction liquid: the water first adding 6 times amount (in crude drug quality) in crude drug, boils 30 minutes, filters; In filtering residue, add the water of 2 times amount (in medicinal residues quality) according to upper method respectively, boil 30 minutes; Merge 3 extracting solution, concentrate to obtain 100mL Chinese medicine extraction liquid;
(2) anaerobic fermentation: add nutritional supplements in Chinese medicine extraction liquid, autoclaving 30 minutes at 121 DEG C, inoculation probiotic bacteria, anaerobic fermentation 40 hours at temperature 40 DEG C, in fermentation liquid, probiotics bacterial number is 6.7 × 10
9cfu/mL.
Embodiment 4
Fermented type Chinese medicine in the present embodiment, with Chinese medicine extraction liquid, nutritional supplements for raw material, obtains through anaerobic fermentation after inoculation probiotic bacteria; Chinese medicine extraction liquid extracts obtained by crude drug through decocting, crude drug 90g, is made up of according to mass ratio 2:2.1:1.05 Radix Isatidis, the Radix Astragali and Herba Epimedii; Nutritional supplements is made up of 3 grams of bean cake, 3 grams of brown sugar and 1.5 grams of peptones, probiotic bacteria is for be made up of according to number ratio 2:3:2:3 streptococcus thermophilus, Lactobacillus plantarum, enterococcus faecalis, yeast, the preparation method of the inoculum concentration of probiotic bacteria, viable count and fermented type Chinese medicine is with embodiment 3, and in fermentation liquid, probiotics bacterial number is 4.2 × 10
9cfu/mL.
Embodiment 5
Fermented type Chinese medicine in the present embodiment, with Chinese medicine extraction liquid, nutritional supplements for raw material, obtains through anaerobic fermentation after inoculation probiotic bacteria; Chinese medicine extraction liquid extracts obtained by crude drug through decocting, crude drug 100g, is made up of according to mass ratio 2.05:2:1.1 Radix Isatidis, the Radix Astragali and Herba Epimedii; Nutritional supplements is made up of 5 grams of bean cake, 5 grams of brown sugar and 2.5 grams of Carnis caprae seu ovis peptones, probiotic bacteria is for be made up of according to number ratio 3:2:3:2 streptococcus thermophilus, Lactobacillus plantarum, enterococcus faecalis, yeast, the preparation method of the inoculum concentration of probiotic bacteria, viable count and fermented type Chinese medicine is with embodiment 3, and in fermentation liquid, probiotics bacterial number is 6.9 × 10
9cfu/mL.
Embodiment 6
Fermented type Chinese medicine in the present embodiment, with Chinese medicine extraction liquid, nutritional supplements for raw material, obtains through anaerobic fermentation after inoculation probiotic bacteria; Chinese medicine extraction liquid extracts obtained by crude drug through decocting, crude drug 89g, is made up of according to mass ratio 2:2.05:1.05 Radix Isatidis, the Radix Astragali and Herba Epimedii; Nutritional supplements is made up of 3.9 grams of bean cake, 3.8 grams of brown sugar and 1.8 grams of peptones, probiotic bacteria is for be made up of according to number ratio 1.5:2.5:1.5:2.5 streptococcus thermophilus, Lactobacillus plantarum, enterococcus faecalis, yeast, the preparation method of the inoculum concentration of probiotic bacteria, viable count and fermented type Chinese medicine is with embodiment 3, and in fermentation liquid, probiotics bacterial number is 4.8 × 10
9cfu/mL.
Embodiment 7
Fermented type Chinese medicine in the present embodiment, except probiotic bacteria be to be made up of according to number ratio 1.5:2.5:1.5:2.5 streptococcus thermophilus, Lactobacillus plantarum, enterococcus faecalis, yeast except, other are all with embodiment 3, and in fermentation liquid, probiotics bacterial number is 6.2 × 10
9cfu/mL.
Embodiment 8
Fermented type Chinese medicine in the present embodiment, except probiotic bacteria be to be made up of according to number ratio 1:1:1:1 streptococcus thermophilus, Lactobacillus plantarum, enterococcus faecalis, yeast except, other are all with embodiment 3, and in fermentation liquid, probiotics bacterial number is 5.5 × 10
9cfu/mL.
Embodiment 9
Fermented type Chinese medicine in the present embodiment, except probiotic bacteria be to be made up of according to number ratio 1:1:1 streptococcus thermophilus, Lactobacillus plantarum, enterococcus faecalis except, other are all with embodiment 1, and in fermentation liquid, probiotics bacterial number is 6.2 × 10
9cfu/mL.
Embodiment 10
Fermented type Chinese medicine in the present embodiment, except probiotic bacteria be to be made up of according to number ratio 1.5:1 streptococcus thermophilus, Lactobacillus plantarum except, other are all with embodiment 1, and in fermentation liquid, probiotics bacterial number is 5.8 × 10
9cfu/mL.
According to correlation test data, effect of the present invention is described below.
Testing data:
(1) to cure the disease aspect:
1, cure mainly: infectious bursal disease.
2, case statistics: treat in 164 examples, broiler chicken 124 example, Egg chickens 40 example; The course of disease is the shortest 1 day, the longest 5 days.
3, Therapeutic Method: the every 250mL of fermented type Chinese medicine embodiment 1 prepared is watered 1000 jin and freely drinks (also can calculate the once concentrated dispensing of total amount of drinking water every day) to sick chicken.3 ~ 5 days each courses for the treatment of, the course for the treatment of, interval 3 ~ 5 days can again with the next course for the treatment of.After general 1 course for the treatment of, clinical symptoms disappears substantially, re-uses 1 course for the treatment of afterwards, consolidate curative effect, in case recurrence.
4, efficacy assessment standard:
Cure: symptom of diarrhea disappear, the chicken group mental status and feed intake normal;
Take a turn for the better: diarrhea rate reduces, and the mental status and feed intake reach normal substantially;
Invalid: not reach improvement target person.
5, therapeutic effect:
The shortest 2 days, the longest 5 days, 125 examples are cured in medication, account for 76.2%; Take a turn for the better 28 examples, accounts for 17.1%; Invalid 11 examples, account for 6.7%; Total effective rate 93.3%.
Model case:
Case 1: broiler field, township of Henan Province, morbidity broiler 29 age in days, sickness rate 80%, spirit is depressed, and feed intake reduces, and drinking-water increases, and some is from anus pecking door, arranges white water sample loose stool, and severe one is dewatered, and does not rise, extremely feeble sleepingly; Cut open the visible fabricius bursa occurrence characteristics sexually transmitted disease (STD) of inspection to become, fabricius bursa be yellow glue peptone sample edema, matter firmly, mucosa is coated with cream-colored fibrinous exudate.The fermented type Chinese medicine adopting embodiment 2 to prepare concentrates dispensing 2 times sooner or later, and have positive effect after 2 days, after 5 days, clinical symptoms all disappears.
Case 2: broiler field, Henan Province town, morbidity broiler 27 age in days, sickness rate 60%, symptom is lassitude, polydipsia, arranges white color watery stool, fabricius bursa inflammation, necrosis, and the chicken that dies of illness performance dehydration, lower limb and chest muscle often have hemorrhage, and color is dark red.The fermented type Chinese medicine adopting embodiment 3 to prepare concentrates dispensing 2 times sooner or later, and have positive effect after 1 day, after 4 days, clinical symptoms all disappears.
Case 3: broiler field, township of Henan Province, morbidity broiler 25 age in days, sickness rate 85%, lassitude, feed intake reduces, and drinking-water increases, and arranges white water sample loose stool, and severe one is dewatered, extremely feeble; Cut open the visible fabricius bursa inflammation of inspection, necrosis, mucosa is coated with cream-colored fibrinous exudate.Adopt fermented type Chinese medicine prepared by embodiment 4, sooner or later concentrate dispensing 2 times, have positive effect after 2 days, after 6 days, clinical symptoms all disappears.
Case 4: laying hen field, township of Henan Province, morbidity age in days 30, sickness rate 70%, sick chicken lassitude, drinking-water increases, and feed intake reduces, and arranges white water sample loose stool, sick severe one and the chicken that dies of illness performance dewatering symptom; Cut open the visible fabricius bursa inflammation of inspection, necrosis, atrophy, renal swelling is swollen, renal tubules and ureter are full of white urate.Adopt fermented type Chinese medicine prepared by embodiment 5, sooner or later concentrate dispensing 2 times, have positive effect after 2 days, after 6 days, clinical symptoms all disappears.
(2) diseases prevention aspect:
The every 250mL of fermented type Chinese medicine embodiment 1 prepared is watered 2000 jin and freely drinks to sick chicken, and once, young chicken every 2 weeks feedings once for chickling every 1 week feeding.Carry out contrast test in broiler field, Henan Province town, select 43 batches of totally 1720 chickling feedings, by above-mentioned requirements feeding, compared with 1720 chickling matched groups of not this medicine of feeding, disease incidence does not reduce by 28.7 ~ 34.9% not etc., and average attack rate reduces by 31.8%.
In above-described embodiment, bean cake, brown sugar, peptone all can adopt food stage.Wherein, peptone (peptone) is a kind of organic compound, is be flaxen powder by dry outward appearance after the acid of meat, casein or gelatin or protease hydrolysis, has the special breath of meat perfume (or spice).Protein also can form peptone after acid, alkali or proteases for decomposing.
These are only the specific embodiment of the present invention; but protection scope of the present invention is not limited to this; any person skilled in the art, in the technical scope that the present invention discloses, can carry out easily changing or replacing, but should be encompassed in protection scope of the present invention.
Claims (10)
1. fermented type Chinese medicine, with Chinese medicine extraction liquid, nutritional supplements for raw material, obtain through anaerobic fermentation after inoculation probiotic bacteria, it is characterized in that: described nutritional supplements is made up of bean cake, brown sugar and peptone, in every 80 ~ 100mL Chinese medicine extraction liquid, add bean cake 3 ~ 5g, brown sugar 3 ~ 5g, peptone 1.5 ~ 2.5g; Every 1mL Chinese medicine extraction liquid extracts obtained by 0.8 ~ 1.2g crude drug through decocting, crude drug is made up of Radix Isatidis, the Radix Astragali and Herba Epimedii.
2. fermented type Chinese medicine according to claim 1, is characterized in that: in crude drug, the mass ratio of Radix Isatidis, the Radix Astragali, Herba Epimedii is 2:2:1.
3. fermented type Chinese medicine according to claim 1, is characterized in that: add bean cake 4g, brown sugar 4g, peptone 2g in every 90mL Chinese medicine extraction liquid, and every 1mL Chinese medicine extraction liquid extracts obtained by 1g crude drug through decocting.
4. the fermented type Chinese medicine according to any one of claims 1 to 3, is characterized in that: probiotic bacteria is lactobacillus.
5. the fermented type Chinese medicine according to any one of claims 1 to 3, it is characterized in that: probiotic bacteria is made up of streptococcus thermophilus, Lactobacillus plantarum, enterococcus faecalis and yeast, streptococcus thermophilus, Lactobacillus plantarum, enterococcus faecalis, saccharomycetic number ratio are (1 ~ 3): (1 ~ 3): (1 ~ 3): (1 ~ 3).
6. fermented type Chinese medicine according to claim 1, is characterized in that: the condition of anaerobic fermentation is: fermentation temperature 35 ~ 40 DEG C, fermentation time 40 ~ 56 hours.
7. the preparation method of fermented type Chinese medicine as claimed in claim 1, it is characterized in that: in Chinese medicine extraction liquid, add nutritional supplements, inoculate probiotic bacteria after autoclaving, at temperature 35 ~ 40 DEG C, anaerobic fermentation 40 ~ 56 hours, to obtain final product.
8. the preparation method of fermented type Chinese medicine according to claim 7, is characterized in that: in crude drug, the mass ratio of Radix Isatidis, the Radix Astragali, Herba Epimedii is 2:2:1.
9. the preparation method of fermented type Chinese medicine according to claim 7, is characterized in that: add bean cake 4g, brown sugar 4g, peptone 2g in every 90mL Chinese medicine extraction liquid, and every 1mL Chinese medicine extraction liquid extracts obtained by 1g crude drug through decocting.
10. the preparation method of the fermented type Chinese medicine according to any one of claim 7 ~ 9, is characterized in that: peptone is animal proteinum peptone.
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