CN104904372A - Method for promoting germination of Lilium formolongi seeds - Google Patents
Method for promoting germination of Lilium formolongi seeds Download PDFInfo
- Publication number
- CN104904372A CN104904372A CN201510330340.5A CN201510330340A CN104904372A CN 104904372 A CN104904372 A CN 104904372A CN 201510330340 A CN201510330340 A CN 201510330340A CN 104904372 A CN104904372 A CN 104904372A
- Authority
- CN
- China
- Prior art keywords
- curriculum
- new
- ion beam
- seed
- germination
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
The invention discloses a method for promoting germination of Lilium formolongi seeds. The method comprises the following steps: performing low-energy B<+> ion beam injection to Lilium formolongi seeds, wherein low-energy B<+> ion beam is B<+> ion beam with the energy of 300keV, and the injection dose of the low-energy B<+> ion beam is 2*10<14> ions/cm<2>. The method has the following effects by use of injection of low-energy B<+> (boron) ion beams for treating Lilium formolongi: (1) the out-of-ground date of the seed leaf is 3-5 days early than before; (2) the germination rate is increased greatly; (3) the germination potential is improved by 1-2 times, the germinated buds are unifrom, the emerging time is consistent, and the method has great application value for the seedlings growth of the Lilium formolongi.
Description
Technical field
The invention belongs to biological technical field, relate to a kind of method promoting new PE curriculum seed germination, be specifically related to a kind of method being injected promotion new PE curriculum seed germination by low energy ion beam.
Background technology
New PE curriculum (Lilium ╳ formolongi) is the crossbreed of Lilium longiflorum (L.longiflorum) and Taiwan lily (L.formosanum), be to adopt seminal propagation with the maximum difference of common lily, and completed in 1 year from being seeded into the growth course of blooming.New PE curriculum spend large lobe thick, give off a strong fragrance, pattern is pure white, high temperature resistant, flowering stage is 6 ~ November.Because its breeding time is short, and can seed propagation, sight is high, and reduce production cost, plantation amount at home and abroad constantly increases, and plays an important role to the shortening Lilies breeding cycle.
The seedling-breeding method of new PE curriculum mainly comprises the numerous seedling of seed, tissue culture method, scale cuttage etc.Tissue cultures numerous seedling method and scale cuttage numerous seedling method application difficulty more greatly, not easily popularize and production cost is higher.Seeding method easily carries out, and (new PE curriculum generally needs 7 ~ 8 months from being seeded into bloom, usually sow in February November to next year, field planting in 4 ~ May, 7 ~ September blooms), but the maximum difficult problem of seeding method is that emergence rate is low, seedling management is difficult, and then make seedling few, increase production cost.
Japan from nineteen thirty begin carry out new PE curriculum breeding work, the kind of incubation is divided into napiform root type, napiform root grow directly from seeds take into account type, three kinds, the type that grows directly from seeds, in early days based on napiform root type, in recent years based on the type that grows directly from seeds.China started to introduce a fine variety from 1999, and production is raised variety single.
At present, the seedling growing process of new PE curriculum mainly relies on breaking dormancy, seed disinfection, adjustment sowing media proportioning, adjustment thickness of sowing, reduces the methods such as sowing thickness of earth covering, wherein only have methods for breaking dormancy can increase by the emergence rate of about 10%, all the other method effects are also not obvious.
Summary of the invention
The object of this invention is to provide a kind of method promoting new PE curriculum seed germination.
The invention provides a kind of method promoting new PE curriculum seed germination, comprise the steps: to get new PE curriculum seed, carry out low energy B
+ion beam mutation.
Described low energy B
+ion beam specifically can be the B that energy is 300keV
+ion beam.
Described low energy B
+the implantation dosage of ion beam mutation specifically can be 1 × 10
13ions/cm
2~ 4 × 10
14ions/cm
2.
Described low energy B
+the implantation dosage of ion beam mutation more specifically can be 2 × 10
14ions/cm
2.
Described new PE curriculum can be the type new PE curriculum that grows directly from seeds.
Described new PE curriculum specifically can be lily cultivar ' Augusta ' (' Augusta ') or lily cultivar ' Juliet ' (' Julius ').
In described method, first described new PE curriculum seed is carried out described " low energy B
+ion beam mutation ", then sow and cultivate.
The present invention also protects the application of above arbitrary described method in new PE curriculum breeding.Described new PE curriculum can be the type new PE curriculum that grows directly from seeds.Described new PE curriculum specifically can be lily cultivar ' Augusta ' or lily cultivar ' Juliet '.In described application, first the seed of described new PE curriculum is processed by described method, then sow and cultivate.
The present invention also protects the application of above arbitrary described method in new PE curriculum nursery.Described new PE curriculum can be the type new PE curriculum that grows directly from seeds.Described new PE curriculum specifically can be lily cultivar ' Augusta ' or lily cultivar ' Juliet '.In described application, first the seed of described new PE curriculum is processed by described method, then sow and cultivate.
The present invention also protects the application of above arbitrary described method in new PE curriculum expansion is numerous.Described new PE curriculum can be the type new PE curriculum that grows directly from seeds.Described new PE curriculum specifically can be lily cultivar ' Augusta ' or lily cultivar ' Juliet '.In described application, first the seed of described new PE curriculum is processed by described method, then sow and cultivate.
Ion implantation is with the nearly surface region in ion beam mutation base material, to change the process of surface property.Ion beam is the charged particle rays with certain energy, generally energy is called low energy ion lower than the ion of 1000keV.
The index weighing seed sprouting characteristic mainly contains germination vigor and germination rate.Germination vigor refers to that seed germination experiment initial stage (in fixed date) normal chitting piece number accounts for the percentage for planting experimentally subnumber.Germination vigor is high, then represent that seed vigor is strong, germinates neat, emerges consistent.
The present invention finds, utilizes low energy B
+after (boron) ion beam mutation process new PE curriculum, create following effect: (1) cotyledon is unearthed date 3 ~ 5d all ahead of time; (2) germination rate significantly increases; (3) germination vigor improves 1 ~ 2 times, show as germinate neat, seedling-growing time is more consistent.
The present invention has major application value for the nursery of new PE curriculum.
Accompanying drawing explanation
Fig. 1 is low energy B
+ion implantation is to ' Augusta ' M
1the impact of germination vigor.
Fig. 2 is low energy B
+ion implantation is to ' Juliet ' M
1the impact of germination vigor.
Embodiment
Following embodiment is convenient to understand the present invention better, but does not limit the present invention.Experimental technique in following embodiment, if no special instructions, is conventional method.Test material used in following embodiment, if no special instructions, is and purchases available from routine biochemistry reagent shop.Quantitative test in following examples, all arranges and repeats experiment for three times, results averaged.
Lily cultivar ' Augusta ', lily cultivar ' Juliet ' all belong to the type that grows directly from seeds in new PE curriculum, all purchased from Zhejiang Hongyue Horticultural Corporation.
Ion beam mutation adopts GIC 4,117 2 × 1.7MeV tandem accelerator of Beijing City Radiation Centre to carry out.
Embodiment 1,
Test sample is respectively: lily cultivar ' Augusta ' or lily cultivar ' Juliet '.
Test site is: base, Beijing City Radiation Centre Huairou.
1, select the dried lily bulb seed that kind of grain is full, embryo is clear, disease-free, encase kind of a grain with masking foil, expose embryo, seed is adhered to the target disc of 20cm diameter, target disc is put into target chamber and vacuumizes, then carry out ion beam mutation under vacuum conditions.The parameter of ion beam mutation is as follows: energy is the B of 300keV
+ion beam mutation, implantation dosage is respectively 1 × 10
13ions/cm
2, 5 × 10
13ions/cm
2, 1 × 10
14ions/cm
2, 2 × 10
14ions/cm
2with 4 × 10
14ions/cm
2.The parallel control process not carrying out ion beam mutation is set.Carry out three repeated tests, in each repeated test, each dosage operates 50 seeds.The seed of completing steps 1 is M
1for seed.
2, after completing steps 1, on January 15th, 2014, in the cave dish that peat soil is housed, (peat soil was purchased from Bei Lin Science and Technology Co., Ltd. by planting seed; Peat soil moisture soaks into, and moisture reaches capacity; After program request, earthing 0.5cm, water spray, covered with plastic film, keeps humidity), carry out cultivating (daytime temperature 20 DEG C, night temperature 15 DEG C) at greenhouse by solar heat.Expose seed coat 1 ~ 2mm as germination mark using radicle, calculate germination rate and germination vigor.Percentage of seedgermination=normal chitting piece number/sowing seed number × 100%.Chitting piece number/sowing seed number × 100% of potentiality of seed=after planting the 7th day.
3, on April 2nd, 2014, wait emerge neat after, (nutritive cube is built with culture matrix to the nutritive cube of bore 10cm unify transplanting; 3 parts by volume soil and the 1 parts by volume peat composed of rotten mosses are mixed, obtain culture matrix), carry out cultivating (daytime temperature 20 ~ 25 DEG C, night temperature 15 ~ 18 DEG C) at greenhouse by solar heat, observe its upgrowth situation.
4, on May 12nd, 2014, by little transplantation of seedlings in land for growing field crops (the long 10m of row, line-spacing 30cm, spacing in the rows 30cm), conventional field management.
The M of different disposal group
1for the germination period (namely start germinate date) of seed, leaf period (namely starting the date of long rough leaf) in table 1.New PE curriculum seed M
1after planting seed, the cotyledon of each low energy ion beam processed group is unearthed the date all between February 15 to February 18, and than contrasting Zao 3 ~ 5 days of processed group, and the germination date of each low energy ion beam processed group is relatively concentrated.New PE curriculum seed M
1after planting seed, each low energy ion beam processed group is unearthed to the interval time shorter (being about 30d) that the 1st true leaf occurs from cotyledon, and the interval time that follow-up true leaf occurs is about 10d, 3 ~ 5d more Zao than contrast processed group.
Table 1 M
1for the germination period after planting seed, leaf period (day/moon)
The M of different disposal group
1for the germination rate of seed in table 2 (statistics on March 12).New PE curriculum seed M
1after planting seed, the germination rate of each low energy ion beam processed group is all significantly higher than control group.1 × 10
14ions/cm
2, 2 × 10
14ions/cm
2' Juliet ' percentage of seedgermination the highest (being 92%) of processed group, the germination rate of corresponding control group is 46%.
Table 2 M
1for the germination rate (%) after planting seed
| 1×10 13ions/cm 2 | 5×10 13ions/cm 2 | 1×10 14ions/cm 2 | 2×10 14ions/cm 2 | 4×10 14ions/cm 2 | |
| ' Augusta ' (ion beam mutation) | 70％ | 75％ | 63％ | 76％ | 76％ |
| ' Juliet ' (ion beam mutation) | 86％ | 85％ | 92％ | 92％ | 78％ |
| ' Augusta ' (control treatment) | 38％ | 38％ | 38％ | 38％ | 38％ |
| ' Juliet ' (control treatment) | 46％ | 46％ | 46％ | 46％ | 46％ |
The M of different disposal group
1for the strain rate of seed in table 3 (statistics on May 30).New PE curriculum seed M
1in generation, transplants to land for growing field crops, and the germination rate that its strain rate was added up compared with March 12 has almost no change, and visible low energy ion beam injects process, and to it, from germination to four leaves, wholeheartedly period has no significant effect.
Table 3 M
1for the strain rate (%) after planting seed
' Augusta ' M of different disposal group
1fig. 1 is seen for the planting seed germination rate of the 1 to 7 day.' Augusta ' M1 is for after planting seed, and the germination vigor of each low energy ion beam processed group is all significantly higher than control group.
' Juliet ' M of different disposal group
1fig. 1 is seen for the planting seed germination rate of the 1 to 7 day.' Juliet ' M1 is for after planting seed, and the germination vigor of each low energy ion beam processed group is all significantly higher than control group.
Claims (9)
1. promote a method for new PE curriculum seed germination, comprise the steps: to get new PE curriculum seed, carry out low energy B
+ion beam mutation.
2. the method for claim 1, is characterized in that: described low energy B
+the B of ion beam to be energy be 300keV
+ion beam.
3. method as claimed in claim 2, is characterized in that: described low energy B
+the implantation dosage of ion beam mutation is 1 × 10
13ions/cm
2~ 4 × 10
14ions/cm
2.
4. method as claimed in claim 2, is characterized in that: described low energy B
+the implantation dosage of ion beam mutation is 2 × 10
14ions/cm
2.
5., as the method as described in arbitrary in Claims 1-4, it is characterized in that: described new PE curriculum is the type new PE curriculum that grows directly from seeds.
6., as the method as described in arbitrary in Claims 1-4, it is characterized in that: described new PE curriculum is lily cultivar ' Augusta ' or lily cultivar ' Juliet '.
7. the application of arbitrary described method in new PE curriculum breeding in claim 1 to 6.
8. the application of arbitrary described method in new PE curriculum nursery in claim 1 to 6.
9. the application of arbitrary described method in new PE curriculum expansion is numerous in claim 1 to 6.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510330340.5A CN104904372B (en) | 2015-06-15 | 2015-06-15 | A kind of method promoting new PE curriculum seed germination |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510330340.5A CN104904372B (en) | 2015-06-15 | 2015-06-15 | A kind of method promoting new PE curriculum seed germination |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104904372A true CN104904372A (en) | 2015-09-16 |
| CN104904372B CN104904372B (en) | 2016-08-24 |
Family
ID=54074170
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510330340.5A Active CN104904372B (en) | 2015-06-15 | 2015-06-15 | A kind of method promoting new PE curriculum seed germination |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104904372B (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5735078A (en) * | 1996-02-19 | 1998-04-07 | Japan Atomic Energy Research Institute | Method of producing intergeneric or interspecific hybrids by ion beam irradiation |
| CN101653077A (en) * | 2009-09-07 | 2010-02-24 | 清流县鸿翔农庄农业发展有限公司 | Method for picking tops, culturing balls, adjusting and controlling florescence of new lilium longiflorum |
| CN101874434A (en) * | 2010-06-29 | 2010-11-03 | 中国农业科学院草原研究所 | Production method of roegneria kamoji seeds |
-
2015
- 2015-06-15 CN CN201510330340.5A patent/CN104904372B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5735078A (en) * | 1996-02-19 | 1998-04-07 | Japan Atomic Energy Research Institute | Method of producing intergeneric or interspecific hybrids by ion beam irradiation |
| CN101653077A (en) * | 2009-09-07 | 2010-02-24 | 清流县鸿翔农庄农业发展有限公司 | Method for picking tops, culturing balls, adjusting and controlling florescence of new lilium longiflorum |
| CN101874434A (en) * | 2010-06-29 | 2010-11-03 | 中国农业科学院草原研究所 | Production method of roegneria kamoji seeds |
Non-Patent Citations (4)
| Title |
|---|
| 李智辉等: "新铁炮百合的播种育苗技术研究", 《辽宁农业科学》 * |
| 王卫东等: "低能离子束辐照对小麦萌发及幼苗活力的影响", 《河南农业科学》 * |
| 王新颖等: "新铁炮百合种子萌发的研究", 《种子》 * |
| 韩秀丽等: "新铁炮百合不同品种种子适宜萌发条件的研究", 《林业科学研究》 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104904372B (en) | 2016-08-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Farooq et al. | Enhancing the performance of direct seeded fine rice by seed priming | |
| CN103975811B (en) | A kind of Oryza sativa L. irrigation by infiltration cultural method | |
| CN103766126B (en) | Method for rapidly culturing tea tree seedlings in numerically-controlled greenhouse | |
| CN102907264A (en) | Method for raising watermelon seedlings by dual-root-cutting grafting | |
| CN104584971A (en) | Seedling growing method of moringa oleifera | |
| CN101147447A (en) | Middle saline-alkali soil cotton cultivating method | |
| Guo et al. | Coupling effects of irrigation amount and nitrogen fertilizer type on grain yield, water productivity and nitrogen use efficiency of drip-irrigated maize | |
| CN105123250A (en) | Method for promoting red paeony root seed sprouting and application of method in red paeony root propagation | |
| Singh et al. | Response of crop establishment methods and split application of nitrogen on productivity of rice under irrigated ecosystem. | |
| Santiago-Arenas et al. | Seeding, nitrogen and irrigation management optimize rice water and nitrogen use efficiency | |
| Li et al. | Nutrient and planting modes strategies improves water use efficiency, grain-filling and hormonal changes of maize in semi-arid regions of China | |
| CN101080985A (en) | Selecting and breeding method of 'white felt' Ardisia japonica | |
| CN105594438A (en) | Azalea seedling culture method | |
| CN103798143A (en) | Out-bottle cutting and rooting method for sequoia sempervirens tissue culture seedling | |
| CN109452118A (en) | A kind of radix gentiane dahuvicae type of seeding | |
| CN103703891B (en) | Picriafel-terrae lour seed germination method | |
| Adak et al. | Response of Brassica to microenvironment modification under semi-arid agroecosystem | |
| CN101743908A (en) | Tissue culture, rapid propagation and cultivation method of grevillea banksii | |
| CN104855219A (en) | Dwarf and close planting method for gingko | |
| Mohanty et al. | Response of rice to establishment methods and nutrient management practices in medium land | |
| CN104255236A (en) | Quick seedling cultivation method based on mudar branch or bud cuttage | |
| CN104904372B (en) | A kind of method promoting new PE curriculum seed germination | |
| CN103947431A (en) | Method for culturing seedlings with strong taproots in tobacco cultivation | |
| Awasthi et al. | In vitro study on the reproductive behavior of the endemic and threatened Indian liverwort: Cryptomitrium himalayense Kashyap (Aytoniaceae) | |
| CN109169116B (en) | Planting method for intercropping tillered onions and garlic for promoting garlic blooming |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |