CN101874434A - Production method of roegneria kamoji seeds - Google Patents
Production method of roegneria kamoji seeds Download PDFInfo
- Publication number
- CN101874434A CN101874434A CN 201010212226 CN201010212226A CN101874434A CN 101874434 A CN101874434 A CN 101874434A CN 201010212226 CN201010212226 CN 201010212226 CN 201010212226 A CN201010212226 A CN 201010212226A CN 101874434 A CN101874434 A CN 101874434A
- Authority
- CN
- China
- Prior art keywords
- ion beam
- seeds
- nitrogen ion
- roegneria kamoji
- injection
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Abstract
The invention belongs to the field of seed processing and relates to a method for processing seeds by using a physical method, in particular to a method for injecting roegneria kamoji seeds by utilizing ion beams. The method comprises the following steps of: selecting dry roegneria kamoji seeds and controlling the water content to be 5-14 percent; putting the seeds on a plate of an ion beam injection device; and then, putting the plate in a target chamber to carry out low-energy nitrogen ion beam injection, wherein the injection dose of the low-energy nitrogen ion beams is 500-4,200 dose units, energy in the target chamber is 25 kilo electron volts, the flow magnitude of the nitrogen ion beams is 12 milliamperes, intermittent pulse injection is adopted, the pulse dose each time is 100-500, and the injection spacing interval each time is 20-30s, and the injection vacuum degree is 1.6*10<-2>-2.6*10<-2>. In the method, the germinating ability of the roegneria kamoji seeds is obviously improved, the seeds thrive in a seedling stage, and plants are strong; and the output of a single plant is increased, and the crude protein and the crude fat of the plants are both increased. The method can obviously improve the output and the quality of the roegneria kamoji and has higher application value.
Description
Technical field:
The invention belongs to kind of a grass seeds manufacture field, the present invention relates to furtherly a kind ofly handle grass seed with physical method, is to utilize ion beam to inject grass seed to obtain the method for high-quality seed more specifically.
Technical background:
Roegneria kamoji is a grass family wheat family perennial grass, has the property of blazoning, and adaptability is strong, has moisture-proof, anti gibberellic disease simultaneously, spends more characteristics such as many, and these good characteristics provide valuable genetic resources for the kindred plant breeding of wheat family.Yet as the herbage with feeding value, the output of roegneria kamoji has much room for improvement, and quality remains to be improved.At present, the output of crop or herbage mainly improves in China by fertilising, and still, fertilising can increase its cost, also pollutes the environment, and makes soil deterioration.Last century the mid-80, Hefei Inst. of Plasma Physics, Chinese Academy of Sciences has started ion beam and has injected biological new technology, at subsequently more than 20 year, the ion beam biotechnology was applied in fields such as paddy rice, wheat, cotton respectively, and has cultivated new varieties.But how to utilize ion beam to inject biotechnology applications, improve roegneria kamoji output and quality with expectation in roegneria kamoji seeds.
Summary of the invention
Purpose of the present invention is injected the peculiar property of biological new technology for basis and roegneria kamoji based on the former study ion beam, invents a kind of low energy nitrogen ion beam injection technique of utilizing, to improve roegneria kamoji output and quality.
The object of the invention realizes being finished by following steps:
A) the roegneria kamoji dry seeds of selection full grains, no damage by disease and insect, the control water content is 5%-14%;
B) seed is positioned on the plate of ion beam injection device, the seed embryo is that spiral outer commentaries on classics mode in the center of circle sticks on the plate with double faced adhesive tape with the ware heart upwards;
C) afterwards, plate is put into target chamber carry out the injection of low energy nitrogen ion beam, the implantation dosage of low energy nitrogen ion beam is 500-4200 * 2.6 * 10
13Ions/cm
2, energy is 25 kiloelectron-volts in the target chamber, and nitrogen ion beam stream size is 12 milliamperes, adopts the batch (-type) pulse to inject, and each pulsed dosage is 100-500, and inject blanking time is 20-30s at every turn, the vacuum during injection is 1.6 * 10
-2～2.6 * 10
-2Pa;
Among the present invention, at the described low energy nitrogen ion beam of step c implantation dosage, scope is 3000～4000 * 2.6 * 10
13Ions/cm
2Further, be 500-1000 * 2.6 * 10 in the described low energy nitrogen ion beam of step c implantation dosage scope
13Ions/cm
2, this moment, pulsed dosage ion beam implantation dosage was 500 * 2.6 * 10
13Ions/cm
2
The present invention's advantage compared with the prior art is: the present invention is injected to technological core with the low energy nitrogen ion beam, this technology has the advantage that other chemistry, physical method do not have, little as physiological damage, mutation spectrum is wide, mutation frequency is high, certain repeatability and directionality are arranged, this technology can not cause environmental pollution simultaneously, roegneria kamoji dry seeds of the present invention, and biomass of individual tree is brought up to 147.9g by the 85.1g of contrast, improved 1.74 times, increase rate is higher than other method for treating seeds; Sampling in booting stage, the crude protein content of survey are 13.52%(contrast 12.10%) be 1.12 times that contrast.
Description of drawings
Fig. 1 process frame chart of the present invention.
Specific embodiments
Below with further specifying flesh and blood of the present invention by embodiment in conjunction with the accompanying drawings and correlation technique describes.
Embodiment 1:
As the accompanying drawing signal, select roegneria kamoji 05-11 material, seed moisture content 4% is positioned over seed on the plate of ion beam injection device, and the seed embryo is that spiral outer commentaries on classics mode in the center of circle sticks on the plate with double faced adhesive tape with the ware heart upwards; Afterwards, plate is put into target chamber carry out the injection of low energy nitrogen ion beam, the implantation dosage of low energy nitrogen ion beam is 3000 * 2.6 * 10
13Ions/cm
2, energy is 25 kiloelectron-volts in the target chamber, and nitrogen ion beam stream size is 12 milliamperes, adopts the batch (-type) pulse to inject, and each pulsed dosage is 100, and inject blanking time is 30s at every turn, the vacuum during injection is 1.8 * 10
-2Pa; Inject the cultivation of nursing young plants in hothouses in one week of back, the germination index of its 05-11 is higher by 15.54% than untreated seed, seedling replanting is in the Sha Er of the Ministry of Agriculture open-air science observation experiment of the herbage resource emphasis station of oozing, average temperature of the whole year 5.6 degree, 400 millimeters of average annual rainfalls, 130 days frost-free seasons, soil types chestnut soil and meadow soil.Its tiller number of back turned green in 1 year than untreated increase by 16.53%, and single-strain fresh weight improves 53.47%, and dry weight improves 45.51%.POD to the period of seedling establishment seedling analyzes, and value reaches 887.00, and comparison is according to exceeding 22.88%.
Embodiment 2:
Roegneria kamoji 05-11 material, seed moisture content 10% is positioned over seed on the plate of ion beam injection device, and the seed embryo is that spiral outer commentaries on classics mode in the center of circle sticks on the plate with double faced adhesive tape with the ware heart upwards; Afterwards, plate is put into target chamber carry out the injection of low energy nitrogen ion beam, the implantation dosage of low energy nitrogen ion beam is 4000 * 2.6 * 10
13Ions/cm
2, energy is 25 kiloelectron-volts in the target chamber, and nitrogen ion beam stream size is 12 milliamperes, adopts the batch (-type) pulse to inject, and each pulsed dosage is 100, and inject blanking time is 30s at every turn, the vacuum during injection is 2.6 * 10
-2Pa; Inject the cultivation of nursing young plants in hothouses in one week of back, the germination index of its 05-11 is higher by 21.43% than untreated seed, seedling replanting is in the Sha Er of the Ministry of Agriculture open-air science observation experiment of the herbage resource emphasis station of oozing, average temperature of the whole year 5.6 degree, 400 millimeters of average annual rainfalls, 130 days frost-free seasons, soil types chestnut soil and meadow soil.Its tiller number of back turned green in 1 year than untreated increase by 42.61%, and single-strain fresh weight improves 73.80%, and dry weight improves 54.52%.POD to the period of seedling establishment seedling analyzes, and value reaches 815.67, and comparison is according to exceeding 13.14%.
Embodiment 3:
Roegneria kamoji 05-28 material, seed moisture content 6% is positioned over seed on the plate of ion beam injection device, and the seed embryo is that spiral outer commentaries on classics mode in the center of circle sticks on the plate with double faced adhesive tape with the ware heart upwards; Afterwards, plate is put into target chamber carry out the injection of low energy nitrogen ion beam, the implantation dosage of low energy nitrogen ion beam is 3500 * 2.6 * 10
13Ions/cm
2, energy is 25 kiloelectron-volts in the target chamber, and nitrogen ion beam stream size is 12 milliamperes, adopts the batch (-type) pulse to inject, and each pulsed dosage is 100, and inject blanking time is 30s at every turn, the vacuum during injection is 2 * 10
-2Pa; Inject the cultivation of nursing young plants in hothouses in one week of back, the germination index of its 05-28 is than untreated seed low 10.93%, germinate after 50 days, the seedling height of seedling is higher by 9.17% than untreated, seedling replanting is in the Sha Er of the Ministry of Agriculture open-air science observation experiment of the herbage resource emphasis station of oozing, average temperature of the whole year 5.6 degree, 400 millimeters of average annual rainfalls, 130 days frost-free seasons, soil types chestnut soil and meadow soil.Single-strain fresh weight improved 10.99% in 1 year, and dry weight improves 12.45%.POD to the period of seedling establishment seedling analyzes, and value reaches 718.83, and comparison is according to exceeding 16.07%.Crude protein content improves 8.65%, and crude fat content improves 41.48%, and crude fiber content reduces by 4.42%.
Embodiment 4:
Roegneria kamoji 05-28 material, seed moisture content 13% is positioned over seed on the plate of ion beam injection device, and the seed embryo is that spiral outer commentaries on classics mode in the center of circle sticks on the plate with double faced adhesive tape with the ware heart upwards; Afterwards, plate is put into target chamber carry out the injection of low energy nitrogen ion beam, the implantation dosage of low energy nitrogen ion beam is 700 * 2.6 * 10
13Ions/cm
2, energy is 25 kiloelectron-volts in the target chamber, and nitrogen ion beam stream size is 12 milliamperes, adopts the batch (-type) pulse to inject, and each pulsed dosage is 100, and inject blanking time is 30s at every turn, the vacuum during injection is 1.6 * 10
-2Pa; Inject the cultivation of nursing young plants in hothouses in one week of back, the germination index of its 05-28 is higher by 2.26% than untreated seed, germinate after 50 days, the seedling height of seedling is higher by 5.21% than untreated, seedling replanting is in the Sha Er of the Ministry of Agriculture open-air science observation experiment of the herbage resource emphasis station of oozing, average temperature of the whole year 5.6 degree, 400 millimeters of average annual rainfalls, 130 days frost-free seasons, soil types chestnut soil and meadow soil.Cultivate tiller number comparison in booting stage then according to improving 55.48%, single-strain fresh weight raising 70.36%, dry weight raising 74.70%.CAT to seedling analyzes, and value reaches 50.843 μ .g
-1.Min
-1FW, comparison is according to exceeding 13.23%.The chlorophyll content of seedling reaches 49.48, and comparison is according to improving 4.37%.Crude protein content improves 11.74%, and crude fat content improves 14.59%, and crude fiber content reduces by 3.92%.
Embodiment 5:
Roegneria kamoji 05-28 material, seed moisture content 5% is positioned over seed on the plate of ion beam injection device, and the seed embryo is that spiral outer commentaries on classics mode in the center of circle sticks on the plate with double faced adhesive tape with the ware heart upwards; Afterwards, plate is put into target chamber carry out the injection of low energy nitrogen ion beam, the implantation dosage of low energy nitrogen ion beam is 4200 * 2.6 * 10
13Ions/cm
2, energy is 25 kiloelectron-volts in the target chamber, and nitrogen ion beam stream size is 12 milliamperes, adopts the batch (-type) pulse to inject, and each pulsed dosage is 500, and inject blanking time is 30s at every turn, the vacuum during injection is 1.6 * 10
-2Pa; Inject cultivations of nursing young plants in hothouses in one week of back, grow up to and transplant behind the seedling in the Sha Er of the Ministry of Agriculture open-air science observation experiment of the herbage resource emphasis station of oozing, average temperature of the whole year 5.6 is spent 400 millimeters of average annual rainfalls, 130 days frost-free seasons, soil types chestnut soil and meadow soil.Cultivate plant height comparison in booting stage then according to improving 23.43%, single-strain fresh weight raising 38.22%, dry weight raising 36.47%.
Embodiment 6:
Roegneria kamoji 05-24 material, seed moisture content 5% is positioned over seed on the plate of ion beam injection device, and the seed embryo is that spiral outer commentaries on classics mode in the center of circle sticks on the plate with double faced adhesive tape with the ware heart upwards; Afterwards, plate is put into target chamber carry out the injection of low energy nitrogen ion beam, the implantation dosage of low energy nitrogen ion beam is 700 * 2.6 * 10
13Ions/cm
2, energy is 25 kiloelectron-volts in the target chamber, and nitrogen ion beam stream size is 12 milliamperes, adopts the batch (-type) pulse to inject, and each pulsed dosage is 500, and inject blanking time is 30s at every turn, the vacuum during injection is 1.6 * 10
-2Pa; Inject the cultivation of nursing young plants in hothouses in one week of back, the germination index of its 05-24 is higher by 1.36% than untreated seed, germinate after 50 days, the seedling height of seedling is higher by 5.10% than untreated, seedling replanting is in the Sha Er of the Ministry of Agriculture open-air science observation experiment of the herbage resource emphasis station of oozing, average temperature of the whole year 5.6 degree, 400 millimeters of average annual rainfalls, 130 days frost-free seasons, soil types chestnut soil and meadow soil.Cultivate then plant height comparison in booting stage according to improving 8.56%, tiller number improves 5.86%, and single-strain fresh weight improves 46.39%, and dry weight improves 30.63%, and the SOD of seedling is analyzed, and value reaches 136.22 μ .g
-1.Min
-1FW, comparison is according to exceeding 3.36%, and POD analyzes, and value reaches 2253.67 μ .g
-1.Min
-1FW, comparison is according to exceeding 26.27%, and CAT analyzes, and value reaches 55.82 μ .g
-1.Min
-1FW, comparison is according to exceeding 20.28%.
The specific targets of each embodiment and data see Table 1-table 5
Table 1 low energy nitrogen ion beam injects the influence to roegneria kamoji 05-11 biomass
| Treatment dosage | Contrast (0) | 3000×2.6×10 13ions/ cm 2 | Improve percentage (%) | 4000×2.6×10 13ions/ cm 2 | Improve percentage (%) |
| Germination index | 14.93 | 17.25 | 15.54 | 18.13 | 21.43 |
| Heading stage tiller number | 97.4 | 113.5 | 16.53 | 138.9 | 42.61 |
| Single-strain fresh weight at heading stage (g) | 85.1 | 130.6 | 53.47 | 147.9 | 73.80 |
| Total dry weight at heading stage (g) | 40.06 | 58.29 | 45.51 | 62.06 | 54.52 |
| POD(μ.min -1.g -1FW) | 721.83 | 887.00 | 22.88 | 816.67 | 13.14 |
Table 2 low energy nitrogen ion beam injects the influence to roegneria kamoji 05-28 biomass
| Treatment dosage | Contrast (0) | 3500×2.6×10 13ions/ cm 2 | Improve percentage (%) |
| Germination index | 18.11 | 16.13 | -10.93 |
| Height of seedling | 22.9 | 25 | 9.17 |
| Heading stage tiller number | 126.2 | 123.6 | -2.06 |
| Single-strain fresh weight at heading stage (g) | 131 | 145.4 | 10.99 |
| Total dry weight at heading stage (g) | 63.52 | 71.43 | 12.45 |
| POD(μ.min -1.g -1FW) | 619.33 | 718.83 | 16.07 |
| Crude protein at heading stage (%) | 11.21 | 12.18 | 8.65 |
| Crude fat at heading stage (%) | 1.76 | 2.49 | 41.48 |
| Raw fiber at heading stage (%) | 31.93 | 30.52 | -4.42 |
Table 3 low energy nitrogen ion beam injects the influence to roegneria kamoji 05-28 biomass
| Treatment dosage | Contrast (0) | 700×2.6×10 13ions/ cm 2 | Improve percentage (%) |
| Germination index | 11.48 | 11.74 | 2.26 |
| Height of seedling (cm) | 12.86 | 13.53 | 5.21 |
| Booting stage tiller number | 57.5 | 89.4 | 55.48 |
| Single-strain fresh weight in booting stage (g) | 23.65 | 40.29 | 70.36 |
| Total dry weight in booting stage (g) | 10.75 | 18.78 | 74.70 |
| CAT(μ.min -1.g -1FW) | 44.89 | 50.83 | 13.23 |
| Crude protein in booting stage (%) | 12.10 | 13.52 | 11.74 |
| Crude fat in booting stage (%) | 4.25 | 4.87 | 14.59 |
| Raw fiber in booting stage (%) | 27.56 | 26.48 | -3.92 |
Table 4 low energy nitrogen ion beam injects the influence to roegneria kamoji 05-28 biomass
| Treatment dosage | Contrast (0) | 4200×2.6×10 13ions/ cm 2 | Improve percentage (%) |
| Plant height in booting stage (cm) | 50.53 | 62.37 | 23.43 |
| Booting stage tiller number | 57.5 | 56.9 | -1.04 |
| Single-strain fresh weight in booting stage (g) | 23.65 | 32.69 | 38.22 |
| Total dry weight in booting stage (g) | 10.75 | 14.67 | 36.47 |
Table 5 low energy nitrogen ion beam injects the influence to roegneria kamoji 05-24 biomass
| Treatment dosage | Contrast (0) | 700×2.6×10 13ions/ cm 2 | Improve percentage (%) |
| Germination index | 11.01 | 11.16 | 1.36 |
| Height of seedling (cm) | 8.62 | 9.06 | 5.10 |
| Booting stage plant height | 35.64 | 38.69 | 8.56 |
| Booting stage tiller number | 83.60 | 88.50 | 5.86 |
| Single-strain fresh weight in booting stage (g) | 25.93 | 37.96 | 46.39 |
| Total dry weight in booting stage (g) | 12.08 | 15.78 | 30.63 |
| SOD(μ.min -1.g -1FW) | 131.79 | 136.22 | 3.36 |
| POD(μ.min -1.g -1FW) | 1784.83 | 2253.67 | 26.27 |
| CAT(μ.min -1.g -1FW) | 46.41 | 55.82 | 20.28 |
Claims (3)
1.
The production method of roegneria kamoji seeds is characterized in that, comprises following production stage:
The roegneria kamoji dry seeds of a, selection full grains, no damage by disease and insect, the control water content is 5%-14%;
B, seed is positioned on the plate of ion beam injection device, the seed embryo is that spiral outer commentaries on classics mode in the center of circle sticks on the plate with double faced adhesive tape with the ware heart upwards;
C, afterwards puts into target chamber with plate and carries out the low energy nitrogen ion beam and inject, and the implantation dosage of low energy nitrogen ion beam is 500-4200 * 2.6 * 10
13Ions/cm
2Energy is 25 kiloelectron-volts in the target chamber, and nitrogen ion beam stream size is 12 milliamperes, adopts the batch (-type) pulse to inject, and each pulsed dosage is 100-500, and inject blanking time is 20-30s at every turn, and the vacuum during injection is 1.6 * 10
-2～2.6 * 10
-2Pa.
2.
The production method of roegneria kamoji seeds according to claim 1 is characterized in that, at the described low energy nitrogen ion beam of step c implantation dosage, scope is 3000～4000 * 2.6 * 10
13Ions/cm
2
3.
The production method of roegneria kamoji seeds according to claim 1 is characterized in that, is 500-1000 * 2.6 * 10 in the described low energy nitrogen ion beam of step c implantation dosage scope
13Ions/cm
2, this moment, pulsed dosage ion beam implantation dosage was 500 * 2.6 * 10
13Ions/cm
2
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010212226 CN101874434A (en) | 2010-06-29 | 2010-06-29 | Production method of roegneria kamoji seeds |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201010212226 CN101874434A (en) | 2010-06-29 | 2010-06-29 | Production method of roegneria kamoji seeds |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101874434A true CN101874434A (en) | 2010-11-03 |
Family
ID=43017202
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201010212226 Pending CN101874434A (en) | 2010-06-29 | 2010-06-29 | Production method of roegneria kamoji seeds |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101874434A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104904372A (en) * | 2015-06-15 | 2015-09-16 | 北京市辐射中心 | Method for promoting germination of Lilium formolongi seeds |
| CN105009889A (en) * | 2015-07-07 | 2015-11-04 | 江苏农林职业技术学院 | Building and planting method of evergreen lawn |
| CN106856716A (en) * | 2017-03-02 | 2017-06-20 | 张亮 | A kind of golden larch seed pre-sowing treatment method |
| CN106888620A (en) * | 2017-03-02 | 2017-06-27 | 张瑞明 | A kind of raising old kind of method of survival rate of seedling of agate card |
| CN113439501A (en) * | 2021-06-18 | 2021-09-28 | 中国农业科学院草原研究所 | Method for improving activity of grass seeds |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1547894A (en) * | 2003-05-23 | 2004-11-24 | 中国科学院近代物理研究所 | Fixed point heavy oil beam mutation breeding method for wheat |
-
2010
- 2010-06-29 CN CN 201010212226 patent/CN101874434A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1547894A (en) * | 2003-05-23 | 2004-11-24 | 中国科学院近代物理研究所 | Fixed point heavy oil beam mutation breeding method for wheat |
Non-Patent Citations (1)
| Title |
|---|
| 《安徽农业科学》 20100110 于靖怡等 氮离子束对鹅观草属植物萌发特性及幼苗生长的影响 146-147，162 1-3 第38卷, 第1期 2 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104904372A (en) * | 2015-06-15 | 2015-09-16 | 北京市辐射中心 | Method for promoting germination of Lilium formolongi seeds |
| CN104904372B (en) * | 2015-06-15 | 2016-08-24 | 北京市辐射中心 | A kind of method promoting new PE curriculum seed germination |
| CN105009889A (en) * | 2015-07-07 | 2015-11-04 | 江苏农林职业技术学院 | Building and planting method of evergreen lawn |
| CN105009889B (en) * | 2015-07-07 | 2017-07-11 | 江苏农林职业技术学院 | A kind of planting and establishing method of Evergreen lawn |
| CN106856716A (en) * | 2017-03-02 | 2017-06-20 | 张亮 | A kind of golden larch seed pre-sowing treatment method |
| CN106888620A (en) * | 2017-03-02 | 2017-06-27 | 张瑞明 | A kind of raising old kind of method of survival rate of seedling of agate card |
| CN113439501A (en) * | 2021-06-18 | 2021-09-28 | 中国农业科学院草原研究所 | Method for improving activity of grass seeds |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105794354B (en) | A kind of sweet photo seed float seedling method | |
| CN103222386A (en) | Ginger large-small row dual-bud cultivation method | |
| CN105532232B (en) | A kind of cultural method improving tuniclike psammosilene root emergence rate | |
| CN103039250B (en) | Method for planting callicarpa nudiflora seedlings | |
| CN103891510A (en) | Garlic sprout seed cultivation method | |
| CN101874434A (en) | Production method of roegneria kamoji seeds | |
| CN105145133B (en) | The Upper-middle Reaches of Yangtze River autumn delays eggplant cultivation method | |
| CN104813831B (en) | A kind of indoor fast breeding method of wild barley | |
| CN105409662A (en) | Organic rice and cultivation method therefor | |
| CN104303925A (en) | Method for quickly culturing seedlings of ginkgo trees | |
| CN104956996A (en) | Comprehensive ecological planting and breeding method for grapes, finless eels and earthworms | |
| CN108967192A (en) | A kind of Sweetpotato Viruses Elimination bottle seedling acclimatization and transplants method | |
| CN110800490B (en) | Application of cold plasma treatment in promoting survival and growth of grafted tomatoes | |
| CN109511534B (en) | Berry seed sowing and breeding method | |
| CN110393120A (en) | It is a kind of suitable kind in the cherry sapling multiplication breeding method of Guangxi high and cold mountain area | |
| CN1539258A (en) | TRechnique for growing seedlings of American cross bred plummy apricot | |
| CN107873366A (en) | The method for culturing seedlings of radix fici simplicissimae | |
| Mohanty et al. | Assessment of yield using cono weeder in SRI method of paddy cultivation | |
| CN106576788A (en) | Stress resistance breeding method of wheat | |
| CN109983875B (en) | Method for improving stress resistance of plant seeds | |
| LU500164B1 (en) | Method for improving roegneria kamoji yield and quality | |
| CN103843545A (en) | Method for domesticating and cultivating high-quality rootstock seedlings by utilizing wild eggplant seeds | |
| CN111955293A (en) | Seedling raising method for improving survival rate of seeds | |
| CN105684597A (en) | Notopterygium root seed processing method and black mulching film land cultivating method of notopterygium roots | |
| CN111543198A (en) | Method for grafting lateral branches of tomatoes |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Open date: 20101103 |