CN104892157B - A kind of active alga fertilizer and its production method - Google Patents
A kind of active alga fertilizer and its production method Download PDFInfo
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Abstract
The present invention relates to a kind of active alga fertilizer and preparation method thereof, the preparation method includes digestion, enzymolysis and post-treating and other steps.The key of the preparation method is that the digestion of weak base is carried out before complex enzyme is added, and does not both cause very big destruction to active material in marine alga, is conducive to the preliminary extraction of marine alga again, so as to reduce the consumption of complex enzyme.The marine alga fertilizer product is rich in various active materials such as alginic acid, gibberellin, zeatin, growing for plant can be effectively facilitated, crop yield is improved, while greatly remaining natural degeneration-resistant composition glycine betaine and phenolic compound in marine alga etc., is conducive to improving the resistance of plant.
Description
【Technical field】
The present invention relates to technical field of fertilizer production.Lived more particularly it relates to be prepared by raw material of natural seaweed
Property alga fertilizer method, further relate to described active alga fertilizer.
【Background technology】
Rich in abundant nutritional ingredient in marine alga, the species of wherein active material has as many as tens kinds, including marine alga
Polysaccharide, glycine betaine, endogenous hormones (auximone, gibberellin, zeatin etc.), phenolic compound, mannitol etc., these are effective
Most of composition is present in the cell membrane of marine alga, and directly application is difficult to be absorbed and used by plants, and the elite of alga fertilizer is marine alga
The natural active matter itself being rich in, therefore certain processing technology need to be taken to extract the active component in marine alga, and
Extracting method is very big for the influence of Product Activity material and nutrient composition content.
At present, most of alga fertilizer manufacturers produce marine alga fertilizer using chemical method, are mainly added with strong acid and strong base
Heat, makes alginic cell broken wall, and the greatest drawback of this method is that strong acid and strong base can destroy the work of nutriment in marine alga
Property, the significant losses of marine alga active ingredient are caused, so as to finally influence the using effect of marine alga fertilizer product.Wang Jiaming exists《Hangzhou
Chemical industry》, 40 (4), pp4-9, (2010) mention the reparation technology of alga fertilizer to the activity influences of active skull cap components greatly, and most
The extracting method of difference is exactly this chemical method of strong acid and strong base.CN 104177136A disclose one kind and fertilizer are extracted from marine alga
With the method for active material, complex enzyme hydrolysis extraction and Ultra filtration membrane technology are utilized, it is ensured that the activity of active material.It is of the invention first
Alginic cell wall first is swelled using the method for weak base, a portion nutriment can be extracted, again will not be to active component
Do a lot of damage, recycle enzyme solution further to extract remaining active ingredient, prepare active alga fertilizer, this technology
The extraction process of marine alga active material is optimized, the recovery rate of nutriment in marine alga is improved, the quality of product is improved, can
Realize the upgrading of existing alga fertilizer production technology.
【The content of the invention】
[technical problem to be solved]
It is an object of the invention to provide a kind of active alga fertilizer.
It is a further object to provide the preparation method of active alga fertilizer.
[technical scheme]
The present invention is achieved through the following technical solutions.
The present invention relates to a kind of production method of active alga fertilizer.
The step of production method, is as follows：
A, digestion
The drying marine alga raw material of crushing is well mixed with weak base aqueous solution, then in 50~60 DEG C of temperature and the condition that stirs
It is lower to carry out 1~2h of digestion, obtain a kind of marine alga digestive juice；
B, enzymolysis
The pH of the obtained digestive juices of step A is adjusted to 6.8~7.2 with aqueous citric acid solution, then added with digestive juice
The complex enzyme that quality meter 0.01%~0.10% is made up of cellulase, pectase and papain, is well mixed, then exists
30~40 DEG C of temperature obtains a kind of enzymolysis liquid with carrying out enzymolysis 15~20 hours under conditions of stirring；
C, post processing
The enzymolysis liquid that step B is obtained is centrifuged removing impurity, and its supernatant is soluble solid by being concentrated under reduced pressure to give
Body content is by weight 20%~25% concentrate, and the concentrate is spray-dried, obtains described active alga fertilizer.
A preferred embodiment of the invention, in step, described marine alga are one or more selected from horse hair
The marine alga of algae, sea-tangle or yellow tang.
According to the present invention another preferred embodiment, in step, described weak base be potassium carbonate, saleratus,
Sodium carbonate or sodium acid carbonate；The concentration of described weak base aqueous solution is 0.001~0.004g/ml.
According to another preferred embodiment of the present invention, in step, the marine alga and the mass ratio of the weak base are
5~8:1.
According to another preferred embodiment of the present invention, in stepb, the concentration of the aqueous citric acid solution is 0.2
~0.8g/ml.
According to another preferred embodiment of the present invention, in stepb, described complex enzyme is fine by 18~35 parts by weight
The plain enzyme of dimension, 20~36 parts by weight pectases and 32~46 parts by weight papains are constituted.
According to another preferred embodiment of the present invention, in step C, described centrifugation is to use Horizontal sedimentation
What centrifuge was carried out under conditions of 3000~3500r/min.
According to another preferred embodiment of the present invention, in step C, described being concentrated under reduced pressure is concentrated using triple effect
Equipment is carried out under conditions of evaporation capacity is 1.2~1.6 ton hours.
According to another preferred embodiment of the present invention, in step C, described spray drying is to use press atomization
Drying equipment is under conditions of 300~400 DEG C of EAT, 120~150 DEG C of leaving air temp, inlet amount are 250~300kg/h
Carry out.
The invention further relates to the active alga obtained according to methods described fertilizer, it contains 5~20%K by weight2O, 1~
2%P2O5, 0.5~1.0%N, 30~40% organic matters, 0.05~0.10%Ca, 0.2~0.3%Mg, 6~10% alginic acids with
1500~2000mg/kg plant hormones.
The present invention is described in more detail below.
The present invention relates to a kind of production method of active alga fertilizer.
The step of production method, is as follows：
A, digestion
The drying marine alga raw material of crushing is well mixed with weak base aqueous solution, then in 50~60 DEG C of temperature and the condition that stirs
It is lower to carry out 1~2h of digestion, obtain a kind of marine alga digestive juice；
In the present invention, described marine alga is one or more marine algas for being selected from sargassum, sea-tangle or yellow tang.
Sargassum, sea-tangle and yellow tang are all the products sold in the market.It is using food at present to dry marine alga raw material
Usually used equipment is crushed in product processing technique field, for example by the company of powder equipment Co., Ltd of Shanghaiization three with
The disintegrating apparatus of trade name SCF twin shafts atomizer sale.
It is not particularly critical to dry marine alga raw material grinding particle size, but suitable granularity is then conducive to follow-up digestion process.
Usually, the granularity for drying marine alga is 50~200 mesh.
According to the present invention, described weak base is potassium carbonate, saleratus, sodium carbonate or sodium acid carbonate, preferably carbonic acid
Potassium, saleratus or sodium carbonate, more preferably potassium carbonate or saleratus.
The concentration for the weak base aqueous solution that the present invention is used is 0.001~0.004g/ml.If the weak base aqueous solution concentration
It is too high or too low be all it is inappropriate, because if weak base aqueous solution concentration be higher than 0.004g/ml, then pH value of solution is higher, can break
The activity of natural nutrient substance in bad marine alga；If weak base aqueous solution concentration is less than 0.001g/ml, weak base is swelled to marine alga
Effect is limited, and the enzymolysis process of influence below, active material recovery rate declines.
According to the present invention, the mass ratio of the marine alga and the weak base is 5~8:1.Preferably, the marine alga with it is described weak
The mass ratio of alkali is 6~8:1.It is highly preferred that the mass ratio of the marine alga and the weak base is 7~8:1.
Need to be stirred continuously when digesting using weak base, so as to which marine alga powder particles can fully be contacted with weak base aqueous solution,
Digestion reaction is complete.
In this step, if digestion reaction temperature is less than 50 DEG C, digestion reaction carries out incomplete, alginic cell wall
Swelling action be limited, be unfavorable for the dissolution of nutriment in marine alga；If digestion reaction temperature is higher than 60 DEG C, have in marine alga
The activity of effect composition is damaged, and influences the using effect of alga fertilizer.Therefore, the temperature of digestion reaction is 50~60 DEG C, preferably
It is 52~58 DEG C, more preferably 54~56 DEG C.
Under conditions of 50~60 DEG C of digestion reaction temperature, if digestion time is less than 1h, digestion reaction carries out endless
Entirely, the swelling action of alginic cell wall is limited, and is unfavorable for the dissolution of nutriment in marine alga；If digestion time is more than 2h,
Digestion reaction tends towards stability, and causes the significant wastage of the energy, cost increase.Therefore, the time of digestion reaction is that 1~2h is reasonable
, preferably 1.2~1.8h, more preferably 1.4~1.6h.
B, enzymolysis
The pH of the obtained digestive juices of step A is adjusted to 6.8~7.2 with aqueous citric acid solution, then added with digestive juice
The complex enzyme that quality meter 0.01%~0.10% is made up of cellulase, pectase and papain, is well mixed, then exists
30~40 DEG C of temperature obtains a kind of enzymolysis liquid with carrying out enzymolysis 15~20 hours under conditions of stirring；
In the present invention, the concentration for the aqueous citric acid solution that regulation digestive juice pH is used is 0.2~0.8g/ml.If disappeared
When the pH value for changing liquid is less than 6.8, then the activity of complex enzyme can be reduced；If the pH value of digestive juice is higher than 7.2, it can not reach
The work pH of complex enzyme, reduces enzymolysis efficiency；Therefore, the pH value of digestive juice is favourable for 6.8~7.2.
In the present invention, the effect of the complex enzyme is to decompose the alginate fibre remained in marine alga slag, pectin, albumen
The materials such as matter, so that the nutriment in marine alga is farthest extracted.
According to the present invention, described complex enzyme is made up of cellulase, pectase and papain.
Cellulase (cellulose), numbering EC 3.2.1.4.The complicated enzyme that it is made up of a variety of hydrolases
Many fungies can the plain enzyme of eccrine fiber in system, nature.Cellulase is generally divided into three classes：C1 enzymes, Cx enzymes and β glucosides
Enzyme.C1 enzymes are the enzymes initially worked to cellulose, destroy the crystalline texture of cellulose chain.Cx enzymes are acted on through C1 enzyme activations
Cellulose, decompose β -1,4- glycosidic bonds cellulase.β glucosidases can be by cellobiose, cellotriose and other are low
Molecular fiber dextrin is decomposed into glucose.
The cellulase used in the present invention is the product sold by Su Kehan (Weifang) bioengineering Co., Ltd.
Pectase (pectinase) is a multienzyme complex for decomposing pectin, generally includes protopectinase, pectinesterase
Hydrolase, pectinesterase.Pectic substance is set to decompose completely by their synergy.Natural pectic substance is in protopectinase
Under effect, the pectin of water dissolvable is changed into；Pectin removes methyl esters group by pectinesterase hydrolysis enzymatic, generates pectic acid；
Pectic acid is through polygalacturonase class and pectate lyase class degraded generation galacturonic acid.
The pectase used in the present invention is the pectase produced by Su Kehan (Weifang) bioengineering Co., Ltd.
Papain (Papain), numbering EC 3.4.22.2 belong to thiol protease, the work with protease and esterase
Property, there is wide specificity, there is stronger hydrolysis ability to animal/vegetable protein, polypeptide, ester, acid amides etc., meanwhile, also with conjunction
Into function, protolysate can be synthesized proteinoid.
The papain used in the present invention is the product sold by Pangbo Bioengineering Co Ltd, Nanning.
Preferably, described complex enzyme be by 18~35 part by weight of cellulose enzymes, 20~36 parts by weight pectases and 32~
46 parts by weight papains composition.
The amount of the cellulase, pectase and papain can cause the molten of nutritional ingredient when being less than described scope
Go out speed decline, extend the reaction time, and the waste of raw material can be caused during higher than described scope, cost increase is therefore, above-mentioned
Each enzyme amount scope is suitable.
C, post processing
The enzymolysis liquid that step B is obtained is centrifuged removing impurity, and its supernatant is soluble solid by being concentrated under reduced pressure to give
Body content is by weight 20%~25% concentrate, and the concentrate is spray-dried, obtains described active alga fertilizer.
Purpose of the soluble solid content of the concentrate up to 20%~25% be technical target of the product is met crop should
It is required that, and be conducive to the spray drying of next step, save drying cost.
In the present invention, equipment used in centrifuging is the centrifuge sold in the market, centrifuges condition
All according to illustrated using centrifuge operations specification under conditions of carry out.In this step, described centrifugation point
Carried out from using horizontal decanter centrifuge under conditions of 3000~3500r/min.
In this step, it is described be concentrated under reduced pressure be using triple effect concentrator evaporation capacity be 1.2~1.6 tons/it is small
When under conditions of carry out.Triple effect concentrator is the concentrator sold in the market, and such as medicine equipment is contained by China of Jiangyin City
The concentrator that automated arm Science and Technology Ltd. sells in front of section in Co., Ltd, Changzhou.
By described concentrate it is spray-dried after, produce active alga fertilizer product.
In this step, described spray drying is in EAT 300~400 using pressure spray dryer equipment
DEG C, 120~150 DEG C of leaving air temp, inlet amount be to carry out under conditions of 250~300kg/h.The spray drying that the present invention is used
Equipment is the spray dryer generally sold in the market, and the high benefit of such as Jiangsu Xianfeng Drying Engineering Co., Ltd., Wuxi reaches machine
The spray drying device of tool Co., Ltd sale.
The invention further relates to according to the active alga fertilizer obtained by the inventive method.
According to standard NY/T 1977-2010, NY/T 1976-2010, NY/T 1117-2010 and Q/LXSW 10-2013
The active principle content of active alga fertilizer of the present invention is determined under conditions of product appearance stable homogeneous.
Active alga fertilizer of the present invention contains 5~20%K by weight2O, 1~2%P2O5, 0.5~1.0%N, 30~40%
Organic matter, 0.05~0.10%Ca, 0.2~0.3%Mg, 6~10% alginic acids and 1500~2000mg/kg plant hormones.
Active alga fertilizer of the present invention can be applied fertilizer in the way of foliage-spray：Active alga fertilizer of the present invention is diluted with water 2000
~3000 times, according to 150~250 gram/ha/time foliage-sprays.Can also rush apply or pouring root mode according to 4~6 kilograms/it is public
Hectare/time fertilising.
The administration scope of liquid seaweed extract of the present invention is any crop, such as field crop, fruit tree, vegetables, flowers.
Active alga liquid fertilizer of the present invention can stimulate plant growth, improve crop anti-adversity, improve yield, improve agricultural product
Quality.
[beneficial effect]
The beneficial effects of the invention are as follows：
The present invention is using natural seaweed as raw material, using weak base digestion and cellulase, pectase and papain enzymolysis
After PROCESS FOR TREATMENT, the active component in marine alga is extracted, active alga product is prepared.Weak base digestion can be by the cell membrane of marine alga
Soften and be swelled, on the premise of nutriment activity is not destroyed, discharge a part of active component therein, be follow-up
Complex enzyme zymohydrolysis provides advantage, and enzymolysis process can further extract remaining nutriment in marine alga, improve sea
The recovery rate of active ingredient in algae.Two sections of extraction processes had both improved the active component such as basic element of cell division, plant growth in marine alga
Element, gibberellin, glycine betaine, the content of phenolic compound, are effectively reduced the consumption of complex enzyme again, improve the economic effect of enterprise
Benefit, is breakthrough and upgrading to existing alga fertilizer production technology, new approach is provided for the processing of alga fertilizer.Meanwhile,
The active alga fertilizer of the present invention is while promoting the growing of crop, improving yield, moreover it is possible to strengthen cold-resistant, the drought resisting of crop
And the anti-adversity ability such as freeze proof.If marine alga directly digests with weak base, marine alga digestion is not thorough, exists in solution and does not extract completely
Marine alga block, cause the significant wastage of nutriment and loss in marine alga.If marine alga is directly digested with complex enzyme, enzyme
Solution effect is very limited, and the eucaryotic cell structure of most of marine alga can not be destroyed completely, if wanting to reach the effect thoroughly digested, needs significantly
The consumption of enzyme is improved, the increase of production cost is caused.Weak base digestion and complex enzyme zymohydrolysis are organically combined, can be by sea
Active component is greatly extracted in algae, and the consumption of enzyme can be reduced again, with producing feasibility, so as to play synergy doulbe-sides' victory
Effect.
【Brief description of the drawings】
Fig. 1 is the production technological process of active alga fertilizer of the present invention.
【Embodiment】
The present invention is will be better understood that by following embodiments.
Embodiment 1：Production active alga fertilizer of the present invention
The implementation steps of the embodiment are as follows：
Accompanying drawing 1 is shown in specific production procedure.
A, digestion
Mass ratio according to marine alga and weak base is 6:1, drying sargassum raw material and the concentration of crushing are 0.0034g/ml's
Aqueous sodium carbonate is well mixed, and is then carried out digestion 1.8h under conditions of 58 DEG C of temperature and stirring, is obtained a kind of marine alga and disappear
Change liquid；
B, enzymolysis
The pH of the obtained digestive juices of step A is adjusted to 6.8 with concentration 0.2g/ml aqueous citric acid solution, then added
In terms of digestive juice quality 0.04% complex enzyme, is well mixed, and then carries out enzymolysis 18 under conditions of 30 DEG C of temperature and stirring
Hour, obtain a kind of enzymolysis liquid；
Cellulase that described complex enzyme is sold by 25 parts by weight by Su Kehan (Weifang) bioengineering Co., Ltd, 33
Pectase that parts by weight are sold by Su Kehan (Weifang) bioengineering Co., Ltd and 42 parts by weight are by Nanning Pang Bo bioengineering
The papain composition of Co., Ltd's sale.
C, post processing
The enzymolysis liquid that step B is obtained under conditions of 3000r/min using horizontal decanter centrifuge through centrifuging removing
Impurity, its supernatant is concentrated under reduced pressure to give solvable by using triple effect concentrator under conditions of evaporation capacity is 1.6 ton hours
Property solids content for by weight 20% concentrate, the concentrate through using pressure spray dryer equipment in EAT 400
DEG C, 135 DEG C of leaving air temp, inlet amount be to be spray-dried under conditions of 280kg/h, obtain described active alga fertile.
Detected using the method described in this specification, active alga fertilizer manufactured in the present embodiment is containing by weight
6%K2O, 1.4%P2O5, 0.6%N, 38% organic matter, 0.05%Ca, 0.3%Mg, 6% alginic acid and 1620mg/kg plants swash
Element.
Embodiment 2：Production active alga fertilizer of the present invention
The implementation steps of the embodiment are as follows：
A, digestion
Mass ratio according to marine alga and weak base is 5:1, drying sea-tangle raw material and carbonic acid of the concentration for 0.001g/ml of crushing
Hydrogen aqueous solutions of potassium is well mixed, and then carries out digestion 1.0h under conditions of temperature 50 C with stirring, obtains a kind of marine alga digestion
Liquid；
B, enzymolysis
The pH of the obtained digestive juices of step A is adjusted to 7.0 with concentration 0.4g/ml aqueous citric acid solution, then added
In terms of digestive juice quality 0.10% complex enzyme, is well mixed, and then carries out enzymolysis 16 under conditions of 35 DEG C of temperature and stirring
Hour, obtain a kind of enzymolysis liquid；
Cellulase that described complex enzyme is sold by 18 parts by weight by Su Kehan (Weifang) bioengineering Co., Ltd, 36
Pectase that parts by weight are sold by Su Kehan (Weifang) bioengineering Co., Ltd and 46 parts by weight are by Nanning Pang Bo bioengineering
The papain composition of Co., Ltd's sale.
C, post processing
The enzymolysis liquid that step B is obtained under conditions of 3500r/min using horizontal decanter centrifuge through centrifuging removing
Impurity, its supernatant is concentrated under reduced pressure to give solvable by using triple effect concentrator under conditions of evaporation capacity is 1.2 ton hours
Property solids content for by weight 24% concentrate, the concentrate through using pressure spray dryer equipment in EAT 300
DEG C, 120 DEG C of leaving air temp, inlet amount be to be spray-dried under conditions of 250kg/h, obtain described active alga fertile.
Detected using the method described in this specification, active alga fertilizer manufactured in the present embodiment is containing by weight
14%K2O, 1.6%P2O5, 0.5%N, 30% organic matter, 0.06%Ca, 0.2%Mg, 6% alginic acid and 1500mg/kg plants
Hormone.
Embodiment 3：Production active alga fertilizer of the present invention
The implementation steps of the embodiment are as follows：
A, digestion
Mass ratio according to marine alga and weak base is 8:1, drying yellow tang raw material and carbon of the concentration for 0.004g/ml of crushing
Sour aqueous solutions of potassium is well mixed, and then carries out digestion 1.2h under conditions of temperature 60 C with stirring, obtains a kind of marine alga digestion
Liquid；
B, enzymolysis
The pH of the obtained digestive juices of step A is adjusted to 6.8 with concentration 0.6g/ml aqueous citric acid solution, then added
In terms of digestive juice quality 0.01% complex enzyme, is well mixed, and then carries out enzymolysis 15 under conditions of 40 DEG C of temperature and stirring
Hour, obtain a kind of enzymolysis liquid；
Cellulase that described complex enzyme is sold by 35 parts by weight by Su Kehan (Weifang) bioengineering Co., Ltd, 33
Pectase that parts by weight are sold by Su Kehan (Weifang) bioengineering Co., Ltd and 32 parts by weight are by Nanning Pang Bo bioengineering
The papain composition of Co., Ltd's sale.
C, post processing
The enzymolysis liquid that step B is obtained under conditions of 3000r/min using horizontal decanter centrifuge through centrifuging removing
Impurity, its supernatant is concentrated under reduced pressure to give solvable by using triple effect concentrator under conditions of evaporation capacity is 1.2 ton hours
Property solids content for by weight 25% concentrate, the concentrate through using pressure spray dryer equipment in EAT 300
DEG C, 150 DEG C of leaving air temp, inlet amount be to be spray-dried under conditions of 300kg/h, obtain described active alga fertile.
Detected using the method described in this specification, active alga fertilizer manufactured in the present embodiment is containing by weight
20%K2O, 1.0%P2O5, 1.0%N, 40% organic matter, 0.08%Ca, 0.2%Mg, 10% alginic acid and 2000mg/kg plants
Hormone.
Embodiment 4：Production active alga fertilizer of the present invention
The implementation steps of the embodiment are as follows：
A, digestion
Mass ratio according to marine alga and weak base is 7:1, drying sargassum raw material and carbon of the concentration for 0.002g/ml of crushing
Sour hydrogen sodium water solution is well mixed, and is then carried out digestion 2.0h under conditions of 52 DEG C of temperature and stirring, is obtained a kind of marine alga and disappear
Change liquid；
B, enzymolysis
The pH of the obtained digestive juices of step A is adjusted to 7.0 with concentration 0.8g/ml aqueous citric acid solution, then added
In terms of digestive juice quality 0.02% complex enzyme, is well mixed, and then carries out enzymolysis 20 under conditions of 38 DEG C of temperature and stirring
Hour, obtain a kind of enzymolysis liquid；
Cellulase that described complex enzyme is sold by 30 parts by weight by Su Kehan (Weifang) bioengineering Co., Ltd, 32
Pectase that parts by weight are sold by Su Kehan (Weifang) bioengineering Co., Ltd and 38 parts by weight are by Nanning Pang Bo bioengineering
The papain composition of Co., Ltd's sale.
C, post processing
The enzymolysis liquid that step B is obtained under conditions of 3500r/min using horizontal decanter centrifuge through centrifuging removing
Impurity, its supernatant is concentrated under reduced pressure to give solvable by using triple effect concentrator under conditions of evaporation capacity is 1.4 ton hours
Property solids content for by weight 22% concentrate, the concentrate through using pressure spray dryer equipment in EAT 350
DEG C, 135 DEG C of leaving air temp, inlet amount be to be spray-dried under conditions of 280kg/h, obtain described active alga fertile.
Detected using the method described in this specification, active alga fertilizer manufactured in the present embodiment is containing by weight
5%K2O, 2.0%P2O5, 0.8%N, 32% organic matter, 0.10%Ca, 0.3%Mg, 9% alginic acid and 1680mg/kg plants swash
Element.
Embodiment 5：Production active alga fertilizer of the present invention
The implementation steps of the embodiment are as follows：
A, digestion
Mass ratio according to marine alga and weak base is 6:1, drying sargassum raw material and carbon of the concentration for 0.002g/ml of crushing
Sour aqueous solutions of potassium is well mixed, and then carries out digestion 1.5h under conditions of 54 DEG C of temperature and stirring, obtains a kind of marine alga digestion
Liquid；
B, enzymolysis
The pH of the obtained digestive juices of step A is adjusted to 7.2 with concentration 0.4g/ml aqueous citric acid solution, then added
In terms of digestive juice quality 0.08% complex enzyme, is well mixed, and then carries out enzymolysis 16 under conditions of 32 DEG C of temperature and stirring
Hour, obtain a kind of enzymolysis liquid；
Cellulase that described complex enzyme is sold by 28 parts by weight by Su Kehan (Weifang) bioengineering Co., Ltd, 29
Pectase that parts by weight are sold by Su Kehan (Weifang) bioengineering Co., Ltd and 43 parts by weight are by Nanning Pang Bo bioengineering
The papain composition of Co., Ltd's sale.
C, post processing
The enzymolysis liquid that step B is obtained under conditions of 3000r/min using horizontal decanter centrifuge through centrifuging removing
Impurity, its supernatant is concentrated under reduced pressure to give solvable by using triple effect concentrator under conditions of evaporation capacity is 1.4 ton hours
Property solids content for by weight 24% concentrate, the concentrate through using pressure spray dryer equipment in EAT 350
DEG C, 120 DEG C of leaving air temp, inlet amount be to be spray-dried under conditions of 250kg/h, obtain described active alga fertile.
Detected using the method described in this specification, active alga fertilizer manufactured in the present embodiment is containing by weight
18%K2O, 1.5%P2O5, 0.6%N, 36% organic matter, 0.06%Ca, 0.2%Mg, 8% alginic acid and 1850mg/kg plants
Hormone.
Embodiment 6：Production active alga fertilizer of the present invention
The implementation steps of the embodiment are as follows：
A, digestion
Mass ratio according to marine alga and weak base is 5:1, drying sea-tangle raw material and carbonic acid of the concentration for 0.004g/ml of crushing
Hydrogen aqueous solutions of potassium is well mixed, and then carries out digestion 1.6h under conditions of 56 DEG C of temperature and stirring, obtains a kind of marine alga digestion
Liquid；
B, enzymolysis
The pH of the obtained digestive juices of step A is adjusted to 7.2 with concentration 0.6g/ml aqueous citric acid solution, then added
In terms of digestive juice quality 0.06% complex enzyme, is well mixed, and then carries out enzymolysis 18 under conditions of 36 DEG C of temperature and stirring
Hour, obtain a kind of enzymolysis liquid；
Cellulase that described complex enzyme is sold by 35 parts by weight by Su Kehan (Weifang) bioengineering Co., Ltd, 20
Pectase that parts by weight are sold by Su Kehan (Weifang) bioengineering Co., Ltd and 45 parts by weight are by Nanning Pang Bo bioengineering
The papain composition of Co., Ltd's sale.
C, post processing
The enzymolysis liquid that step B is obtained under conditions of 3500r/min using horizontal decanter centrifuge through centrifuging removing
Impurity, its supernatant is concentrated under reduced pressure to give solvable by using triple effect concentrator under conditions of evaporation capacity is 1.6 ton hours
Property solids content for by weight 22% concentrate, the concentrate through using pressure spray dryer equipment in EAT 400
DEG C, 150 DEG C of leaving air temp, inlet amount be to be spray-dried under conditions of 300kg/h, obtain described active alga fertile.
Detected using the method described in this specification, active alga fertilizer manufactured in the present embodiment is containing by weight
15%K2O, 1.8%P2O5, 0.8%N, 35% organic matter, 0.05%Ca, 0.3%Mg, 8% alginic acid and 1780mg/kg plants
Hormone.
Comparative example 1：Highly basic digestion production alga fertilizer
The implementation steps of the embodiment are as follows：
A, digestion
Mass ratio according to marine alga and highly basic is 5:1, drying sea-tangle raw material and hydroxide of the concentration for 0.1g/ml of crushing
Aqueous solutions of potassium is well mixed, and is then carried out digestion 4h under conditions of 95 DEG C of temperature and stirring, is obtained a kind of marine alga digestive juice；
B, post processing
The digestive juice that step A is obtained under conditions of 3000r/min using horizontal decanter centrifuge through centrifuging removing
Impurity, its supernatant is concentrated under reduced pressure to give solvable by using triple effect concentrator under conditions of evaporation capacity is 1.4 ton hours
Property solids content for by weight 24% concentrate, the concentrate through using pressure spray dryer equipment in EAT 350
DEG C, 120 DEG C of leaving air temp, inlet amount be 250kg/h under conditions of be spray-dried, obtain alga fertilizer.
Detected using the method described in this specification, alga fertilizer manufactured in the present embodiment contains by weight 17%
K2O, 1.2%P2O5, 0.5%N, 10% organic matter, 0.06%Ca, 0.1%Mg, 2% alginic acid and 200mg/kg plant hormones.
The result of the test of the comparative example 1 shows, compared with embodiment 1-6, and comparative example 1 prepares the plant of alga fertilizer
Thing hormone-content is much lower.
Test example 1：Manure trial (tomato)
For studying thing tomato, kind is hair powder 802.
Experiment sets Routine control (CK) and active alga of the present invention fertilizer (A1, A2, A3) foliage-spray 4 is handled, A1, A2,
A3 is active alga fertilizer prepared by embodiment 1,2,3 respectively.
Each 3 repetitions of processing, plot area is 2.0m*2.5m, random alignment, experimental field ploughs, transplants, watering etc. and arranging
Apply completely the same.Proceeded by from May 30 and periodically survey production, each handled and mixing fresh 12, sample is adopted when the 4th fringe fruit is ripe,
Analyze the quality of fruit.Result of the test is listed in Table 1 below.
Table 1：Tomato survey of growth and development table
As can be seen from Table 1, using the growing state of the fertile each processing tomato early stage of active alga of the present invention and to photograph
Than there is notable difference.Investigated in field planting (April 20) the 30th day afterwards (May 19) and (10 plants are investigated per cell, altogether investigation
30 plants), the result is that the tomato for applying active alga fertilizer of the present invention averagely increases 3~5cm than control group plant, cane adds
Thick 0.07~0.15cm, average percentage of fertile fruit improves 5.64~10.02%.Tomato is applied after active alga of the present invention fertilizer simultaneously, can
To prevent the generation of powdery mildew, the powdery mildew of control is serious.
Test example 2：Manure trial (pakchoi)
For studying thing for pakchoi, kind is Jinglu No.7.
Experiment sets 4 processing of Routine control (CK) and active alga of the present invention fertile (A4, A5), and A4, A5 are embodiment respectively
4th, the 5 active alga fertilizer prepared, plot area 10m2, each 3 repetitions of processing, in Growth of Cabbage early stage, mid-term, later stage point
3 foliage-sprays.Experimental plot waters, it is completely the same to prevent and treat the measures such as pest and disease damage.Result of the test is listed in Table 2 below.
The biomass and quality of the different disposal pakchoi of table 2
From table 2 it can be seen that apply the yield of pakchoi and quality of active alga of the present invention fertilizer has bright compared with the control
Aobvious to improve, total biomass, which increases by 0.53~0.61kg, nitrate nitrogen content, increases by 34~52mg/kg, 12~14mg/ of Vc contents increase
100g ,~Water-soluble Total Sugar content increase by 3.1~4.8g/kg.
Claims (8)
1. a kind of production method of active alga fertilizer, it is characterised in that as follows the step of the production method：
A, digestion
The drying marine alga raw material and concentration of crushing are 0.001~0.004g/ml weak base aqueous solution according to mass ratio 5~8:1 mixes
Close uniform, 1~2h of digestion is then carried out under conditions of 50~60 DEG C of temperature and stirring, a kind of marine alga digestive juice is obtained；It is described
Weak base be potassium carbonate, saleratus, sodium carbonate or sodium acid carbonate；
B, enzymolysis
The pH of the obtained digestive juices of step A is adjusted to 6.8~7.2 with aqueous citric acid solution, then added with digestive juice quality
The complex enzyme that meter 0.01%~0.10% is made up of cellulase, pectase and papain, is well mixed, then in temperature
30~40 DEG C, with carrying out enzymolysis 15~20 hours under conditions of stirring, obtain a kind of enzymolysis liquid；
C, post processing
The enzymolysis liquid that step B is obtained is centrifuged removing impurity, and its supernatant is contained by being concentrated under reduced pressure to give soluble solids
The concentrate for by weight 20%~25% is measured, the concentrate is spray-dried, obtain described active alga fertilizer.
2. according to the method described in claim 1, it is characterised in that in step, described marine alga is that one or more are selected from
The marine alga of sargassum, sea-tangle or yellow tang.
3. according to the method described in claim 1, it is characterised in that in stepb, the concentration of the aqueous citric acid solution is 0.2
~0.8g/ml.
4. according to the method described in claim 1, it is characterised in that in stepb, described complex enzyme is by 18~35 parts by weight
Cellulase, 20~36 parts by weight pectases and 32~46 parts by weight papains are constituted.
5. according to the method described in claim 1, it is characterised in that in step C, described centrifugation is using horizontal heavy
Drop centrifuge is carried out under conditions of 3000~3500r/min.
6. according to the method described in claim 1, it is characterised in that in step C, described being concentrated under reduced pressure is dense using triple effect
Contracting equipment is carried out under conditions of evaporation capacity is 1.2~1.6 ton hours.
7. according to the method described in claim 1, it is characterised in that in step C, described spray drying is sprayed using pressure
Mist drying equipment is in 300~400 DEG C of EAT, 120~150 DEG C of leaving air temp, the condition that inlet amount is 250~300kg/h
Lower progress.
8. according to the active alga fertilizer that any claim methods described is obtained in claim 1-7, it is characterised in that it contains
5~20%K by weight2O, 1~2%P2O5, 0.5~1.0%N, 30~40% organic matters, 0.05~0.10%Ca, 0.2~
0.3%Mg, 6~10% alginic acids and 1500~2000mg/kg plant hormones.
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