CN111269044A - Preparation method of seaweed fertilizer - Google Patents
Preparation method of seaweed fertilizer Download PDFInfo
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- CN111269044A CN111269044A CN201911363359.4A CN201911363359A CN111269044A CN 111269044 A CN111269044 A CN 111269044A CN 201911363359 A CN201911363359 A CN 201911363359A CN 111269044 A CN111269044 A CN 111269044A
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- seaweed
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B17/00—Other phosphatic fertilisers, e.g. soft rock phosphates, bone meal
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Cultivation Of Seaweed (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses a preparation method of a seaweed fertilizer, which comprises the following steps: cleaning seaweed, air drying, and pulverizing into pieces; grinding the obtained seaweed pieces to be in a pulp state, adding water, mixing uniformly, and adding a complex enzyme to obtain a wall-broken seaweed suspension; adding algal polysaccharide lyase into the wall-broken algal suspension to obtain a nutrient suspension; centrifuging and filtering the obtained nutrient suspension to obtain nutrient residues; preparing ingredients, adding water to prepare a solution, and mixing the solution with the nutrient residues to obtain the liquid seaweed organic water-soluble fertilizer. The seaweed fertilizer prepared by the method has the advantages that the nutrient components are degraded by the polysaccharide lyase and changed into smaller molecules from macromolecules, so that the nutrition is easier to be absorbed by plants under the condition of not influencing the nutritional function of the seaweed fertilizer, and the fertilizer efficiency is higher.
Description
Technical Field
The invention relates to the field of agriculture and forestry, in particular to a preparation method of a seaweed fertilizer.
Background
The seaweed fertilizer is a fertilizer prepared by processing a marine kelp extract or a certain amount of nitrogen, phosphorus and potassium or trace elements. The active ingredients extracted from the seaweed mainly comprise natural plant bioactive substances for promoting the growth and development of plants, and various mineral nutrient elements enriched by the seaweed, including seaweed polysaccharide, phenolic polymeric compounds, mannitol, betaine, plant natural growth regulating substances (gibberellin, indoleacetic acid, cytokinin, abscisic acid, salicylic acid and the like), major elements (nitrogen, phosphorus and potassium) and other trace elements (iron, zinc, boron and molybdenum) and the like. Research shows that the seaweed extract has a general yield increasing effect on crops such as vegetables, fruit trees, grains and the like.
Although the seaweed extract has an excellent effect as a fertilizer, there are some problems. One of the main problems is that the seaweed fertilizer obtained by the existing seaweed fertilizer preparation process has low absorption and utilization rate of seaweed active ingredients by plants due to large molecules of the active ingredients.
Disclosure of Invention
In view of the above-mentioned shortcomings, the present invention provides a method for preparing a seaweed fertilizer with low molecular weight nutrients.
The embodiment of the invention adopts the following technical scheme:
the preparation method of the seaweed fertilizer is characterized by comprising the following steps: cleaning and airing the seaweed raw material, and crushing the seaweed raw material into seaweed pieces; grinding the obtained seaweed into slurry, adding water, mixing, and adding complex enzyme preparation to obtain wall-broken seaweed suspension; adding algal polysaccharide lyase preparation into the wall-broken algal suspension to obtain nutrient suspension; centrifuging and filtering the obtained nutrient suspension to obtain nutrient residues; preparing a preparation solution and mixing the preparation solution with the nutrient residues to obtain the liquid seaweed organic water-soluble fertilizer.
In accordance with one aspect of the present invention, the algal polysaccharide lyase comprises a lyase that specifically cleaves β -D-mannuronic acid linked to a 1,4 glycosidic bond and a lyase that specifically cleaves α -L-guluronic acid linked to a 1,4 glycosidic bond.
According to one aspect of the invention, the algal polysaccharide lyase preparation further comprises one or more of aldolase, nuclease, lysozyme, carboxypeptidase, pyruvate decarboxylase, and combinations thereof.
According to one aspect of the invention, the complex enzyme preparation comprises cellulase and pectinase.
According to one aspect of the invention, the complex enzyme preparation further comprises one or two of amylase and glucolase.
According to one aspect of the invention, the seaweed material is one or more of seaweed, brown algae, kelp and leafy algae.
According to one aspect of the invention, the seaweed is shredded into pieces of less than or equal to 3 cm.
According to one aspect of the invention, the resulting pieces of seaweed are ground to a slurry and mixed well with water in an amount of 5 to 8 times the weight of the pieces of seaweed.
In accordance with one aspect of the invention, the dosing solution comprises: preparing ingredients, adding water to obtain an ingredient solution, wherein the ingredients are urea and P2O5、K2O、Ca(NO3)2And one or more of MgO.
In any of the above schemes, the preparation method of the seaweed fertilizer further comprises the following steps: concentrating and spraying the liquid seaweed organic water-soluble fertilizer, and drying by hot air to obtain powder seaweed organic water-soluble fertilizer.
The implementation of the invention has the advantages that:
the seaweed fertilizer prepared by the method of the invention contains nutrient components which are degraded by seaweed polysaccharide lyase, and the final product of the reaction is a mixture of a series of oligosaccharides. The viscosity of the algal polysaccharide is high, and after the alginate lyase is added, the algal polysaccharide is degraded quickly, the viscosity is reduced, and the activity is higher. Is more beneficial to be absorbed by plants. Under the condition of not influencing the nutritional function of the plant, the plant can absorb the nutrition more easily, and the fertilizer efficiency is higher.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1:
a preparation method of a seaweed fertilizer comprises the following steps of cleaning 3 jin of seaweed, 2 jin of brown algae, 4 jin of kelp and 1 jin of leafy algae, drying in the air, repeating the steps for three times, crushing into 3cm small blocks to form seaweed fragments, grinding the obtained seaweed fragments into slurry, adding 50 jin of water, uniformly mixing, adding a complex enzyme to obtain a wall-broken seaweed suspension, wherein the complex enzyme is formed by combining cellulase and pectinase, adding a seaweed polysaccharide lyase into the obtained wall-broken seaweed suspension to obtain a nutrient suspension, the seaweed polysaccharide lyase is formed by combining aldolase, nuclease, a lyase for specifically cracking β -D-mannuronic acid connected with 1,4 glycosidic bonds and a lyase for specifically cracking α -L-guluronic acid connected with 1,4 glycosidic bonds, centrifuging and filtering the obtained nutrient suspension to obtain nutrient residues, preparing ingredients, adding water to prepare a solution, and mixing the solution with the nutrient residues to obtain a liquid seaweed organic water-soluble fertilizer, wherein the ingredients are urea and MgO.
Example 2:
a preparation method of a seaweed fertilizer comprises the following steps of washing 5 jin of seaweed, drying in the air, repeating the steps for four times, crushing the seaweed into small pieces of 0.8cm to form seaweed fragments, grinding the obtained seaweed fragments into slurry, adding 30 jin of water, uniformly mixing, adding a complex enzyme to obtain a wall-broken seaweed suspension, combining the complex enzyme with cellulose and amylase to obtain the wall-broken seaweed suspension, adding seaweed polysaccharide lyase to the wall-broken seaweed suspension to obtain a nutrient suspension, combining the seaweed polysaccharide lyase with aldolase and nuclease specificity to cleave β -D-mannuronic acid with 1,4 glycosidic bonds and the lyase with α -L-guluronic acid with specificity to cleave 1,4 glycosidic bonds, centrifuging and filtering the obtained nutrient suspension to obtain nutrient residues, preparing an ingredient, adding water to prepare a solution, mixing the solution with the nutrient residues to obtain a liquid seaweed organic water-soluble fertilizer, and drying the liquid seaweed organic water-soluble fertilizer after concentrating and spraying organic hot air to obtain the seaweed water-soluble fertilizer.
Example 3:
a preparation method of a seaweed fertilizer comprises the following steps of washing and drying 5 jin of leafy seaweed, repeating the steps for six times, crushing the leafy seaweed into small pieces of 2cm to form seaweed fragments, grinding the obtained seaweed fragments into slurry, adding 25 jin of water, uniformly mixing, adding a complex enzyme to obtain a wall-broken seaweed suspension, combining the complex enzyme by three enzymes of pectinase, amylase and glucosase, adding seaweed polysaccharide lyase into the wall-broken seaweed suspension to obtain a nutrient suspension, combining the seaweed polysaccharide lyase by carboxypeptidase, pyruvate decarboxylase, lyase specifically cracking β -D-mannuronic acid connected by 1,4 glycosidic bonds and lyase specifically cracking α -L-guluronic acid connected by 1,4 glycosidic bonds, centrifuging and filtering the obtained nutrient suspension to obtain nutrient residues, preparing an ingredient, adding water to prepare a solution, mixing the solution with the nutrient residues to obtain a liquid seaweed organic water-soluble fertilizer, mixing the liquid seaweed organic water-soluble fertilizer by two ingredients of urea and MgO, concentrating and spraying hot air drying to obtain the seaweed organic water-soluble fertilizer.
Example 4:
a preparation method of a seaweed fertilizer comprises the following steps of cleaning 3 jin of brown algae, 4 jin of kelp and 3 jin of leafy algae, drying in the air once, crushing into small blocks of 1.5cm to form seaweed fragments, grinding the seaweed fragments into slurry, adding 80 jin of water, mixing uniformly, adding a complex enzyme to obtain a wall-broken seaweed suspension, combining the complex enzyme with three enzymes of cellulase, amylase and glucosidase, adding a seaweed polysaccharide lyase into the wall-broken seaweed suspension to obtain a nutrient suspension, combining the seaweed polysaccharide lyase with seven enzymes of aldolase, nuclease, lysozyme, carboxypeptidase, pyruvate decarboxylase, specifically lyase for cleaving β -D-mannuronic acid connected by 1,4 glycosidic bonds and lyase for cleaving α -L-guluronic acid connected by 1,4 glycosidic bonds, centrifuging and filtering the obtained nutrient suspension to obtain nutrient residues, preparing ingredients, adding water to prepare a solution, mixing the solution with the nutrient residues to obtain a liquid seaweed organic water-soluble fertilizer, and drying the seaweed organic water-soluble fertilizer after the ingredients are combined by urea, P2O O, Ca (3) and MgO (No. 2).
Example 5:
a process for preparing the seaweed fertilizer includes such steps as washing seaweed (5 jin), brown algae (2 jin) and kelp (3 jin) in advance, drying in the air, repeating said steps 5 times, pulverizing to become small blocks (0.5 cm) to become seaweed powder, grinding, mixing with water (70 jin), adding composite enzyme (β -D-mannuronic acid lyase, pectase and glucolase), cracking seaweed polysaccharide (β -D-mannuronic acid) to obtain nutritive suspension, centrifugal filtering to obtain nutritive dregs, adding water, preparing solution, mixing with nutritive dregs, and spray drying to obtain seaweed organic water-soluble fertilizer (urea, P2O5, K2, Ca 3, NO3, etc. although it is common in industrial field, it is a water-soluble fertilizer (O, NO), and spray drying.
The implementation of the invention has the advantages that:
the seaweed fertilizer prepared by the method has the advantages that the nutrient components are degraded by the polysaccharide lyase and changed into smaller molecules from macromolecules, so that the nutrition is easier to be absorbed by plants under the condition of not influencing the nutritional function of the seaweed fertilizer, and the fertilizer efficiency is higher.
The above description is only an embodiment of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention disclosed herein are intended to be covered by the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the appended claims.
Claims (10)
1. The preparation method of the seaweed fertilizer is characterized by comprising the following steps:
cleaning and airing the seaweed raw material, and crushing the seaweed raw material into seaweed pieces;
grinding the obtained seaweed into slurry, adding water, mixing, and adding complex enzyme preparation to obtain wall-broken seaweed suspension;
adding algal polysaccharide lyase preparation into the wall-broken algal suspension to obtain nutrient suspension;
centrifuging and filtering the obtained nutrient suspension to obtain nutrient residues;
preparing a material mixing solution and mixing the material mixing solution and the nutrient residues to obtain the liquid seaweed organic water-soluble fertilizer.
2. The method as claimed in claim 1, wherein the algal polysaccharide lyase comprises a lyase that specifically cleaves β -D-mannuronic acid linked to 1,4 glycosidic bonds and a lyase that specifically cleaves α -L-guluronic acid linked to 1,4 glycosidic bonds.
3. The method as claimed in claim 2, wherein the algal polysaccharide lyase further comprises one or more of aldolase, nuclease, lysozyme, carboxypeptidase, and pyruvate decarboxylase.
4. The method for preparing the seaweed fertilizer as claimed in claim 1, wherein the complex enzyme preparation comprises cellulase and pectinase.
5. The method for preparing the seaweed fertilizer as claimed in claim 4, wherein the complex enzyme preparation further comprises one or a combination of amylase and glucosidase.
6. The method for preparing the seaweed fertilizer as claimed in claim 1, wherein the seaweed material is one or more of seaweed, brown algae, kelp and leafy algae.
7. The method of claim 1, wherein the seaweed is broken into pieces of less than or equal to 3 cm.
8. The method for preparing a seaweed fertilizer as claimed in claim 1, wherein the obtained seaweed pieces are ground into slurry, and water is added to the slurry to mix uniformly, the weight of the water is 5-8 times of the weight of the seaweed pieces.
9. The method for preparing the seaweed fertilizer as claimed in claim 1, wherein the preparing the ingredient solution comprises: preparing ingredients, adding water to obtain an ingredient solution, wherein the ingredients are urea and P2O5、K2O、Ca(NO3)2And one or more of MgO.
10. The method for preparing a seaweed fertilizer according to any one of claims 1 to 9, wherein the method for preparing a seaweed fertilizer further comprises the steps of: concentrating and spraying the liquid seaweed organic water-soluble fertilizer, and drying by hot air to obtain powder seaweed organic water-soluble fertilizer.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201911363359.4A CN111269044A (en) | 2019-12-26 | 2019-12-26 | Preparation method of seaweed fertilizer |
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| CN201911363359.4A CN111269044A (en) | 2019-12-26 | 2019-12-26 | Preparation method of seaweed fertilizer |
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| CN111269044A true CN111269044A (en) | 2020-06-12 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113620730A (en) * | 2021-09-27 | 2021-11-09 | 山东恒来源农业科技有限责任公司 | Organic fertilizer formula for roses |
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| CN102775235A (en) * | 2012-08-16 | 2012-11-14 | 北京雷力农用化学有限公司 | Algin high-tower compound fertilizer and preparation method thereof |
| CN110183266A (en) * | 2019-04-17 | 2019-08-30 | 青岛明月蓝海生物科技有限公司 | A kind of small molecule alginic acid microbial manure |
| CN110526771A (en) * | 2019-08-28 | 2019-12-03 | 广东瑞丰生态环境科技股份有限公司 | A kind of high tower compound fertilizer and preparation method thereof of addition seaweed enzymatic hydrolysis dry powder |
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2019
- 2019-12-26 CN CN201911363359.4A patent/CN111269044A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102775235A (en) * | 2012-08-16 | 2012-11-14 | 北京雷力农用化学有限公司 | Algin high-tower compound fertilizer and preparation method thereof |
| CN110183266A (en) * | 2019-04-17 | 2019-08-30 | 青岛明月蓝海生物科技有限公司 | A kind of small molecule alginic acid microbial manure |
| CN110526771A (en) * | 2019-08-28 | 2019-12-03 | 广东瑞丰生态环境科技股份有限公司 | A kind of high tower compound fertilizer and preparation method thereof of addition seaweed enzymatic hydrolysis dry powder |
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| 于新 等: "《天然食品添加剂》", 31 March 2014, 中国轻工业出版社, pages: 50 - 51 * |
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| 鲍时翔 等: "《海洋微生物学》", 30 April 2008, 中国海洋大学出版社, pages: 299 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113620730A (en) * | 2021-09-27 | 2021-11-09 | 山东恒来源农业科技有限责任公司 | Organic fertilizer formula for roses |
| CN113620730B (en) * | 2021-09-27 | 2022-08-23 | 山东恒来源农业科技有限责任公司 | Organic fertilizer formula for roses |
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Application publication date: 20200612 |