CN104887539A - Rhamnolipid-containing personal cleaning product and preparing method thereof - Google Patents
Rhamnolipid-containing personal cleaning product and preparing method thereof Download PDFInfo
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Abstract
The invention discloses a rhamnolipid-containing personal cleaning product and a preparing method thereof. The preparing method includes: subjecting a bacterium, Pseudomonas aeruginosa, to fermentation cultivation via a non-vertical fermentation tank so as to convert fresh vegetable oil into rhamnolipid, performing extracting, refining and concentrating to obtain rhamnolipid concentrate containing 90% to 95% of rhamnolipid, directly adding 0.1 to 0.5 part of citric acid, as pH regulator, 0.1 to 0.5 part of peppermint water, as flavoring agent, and the balance of distilled water or deionized water, as final concentrate viscosity regulator, into the 50 to 95 parts of the rhamnolipid concentrate, performing full mixing, allowing standing, and performing degassing to obtain the personal cleaning product. The pH is adjusted to 5.0 to 10.0. The repeated trials on the personal cleaning product show that the personal cleaning product has evident technical effect on skin cleaning and post-cleaning tactile improving and is widely applicable.
Description
Technical field
The present invention relates to technical field prepared by hygienic cleansing product, be specifically related to a kind of technical field utilizing rhamnolipid to prepare cleaning supplies.
Background technology
The mankind just understand since ancient times, exquisite Personal hygiene not only can improve Body Comfort Index, prevent disease the essential condition of keeping fit especially, clean is also beautiful prerequisite, job market of today is young all especially lies in individual clean hygiene with the personage that likes to be beautiful, keep the clean salubrious of skin surface and hair, externally can also establish the personal image of good health, promote self-confident.Visible, personal cleanliness's work is not put things right once and for all, need all carry out every day, because the dirt on our skin and hair constantly produces, the perspiration that dirt is mainly discharged from aging exuviae skin cell debris, sweat gland, sebum that sebaceous gland is discharged and the dust sticking to body surface.
Due to the existence of sebum, make the very difficult clean water of these dirts thorough, this is a common recognition out of question, its reason is that profit is clearly demarcated, there is no affinity between the two, therefore amphipathic by surfactant, develops to help to improve personal cleanliness's articles for use for the purpose of the efficiency taken out stains, seems necessary.
Current, personal cleanliness's articles for use that market has been released, mainly three generations is divided into: the first generation from the difference of the time sequencing occurred and core functional component, the early stage mankind mainly adopt, derive from natural plant raw material such as long Fructus Gleditsia, fruit such as circle Fructus Gleditsia and the withered powder of tea etc., wherein saponin is the main composition with clean dirt digestion; The second filial generation, modern age, the mankind mainly adopted, and derive from the oils and fats of animal and the alkali fertile fancy soap that boils of heat altogether, wherein soap is the main composition with clean decontamination; The third generation, the current mankind mainly adopt, and derive from the commercial synthesis surfactant of petrochemical by-product, such as stearic acid, Sulfonates, quaternary ammonium salt, betaines, polyethers, fatty acid glycerine esters etc.
The crowd that the personal cleansing product of first generation natural plant raw material use at present is considerably less, owing to there is the shortcoming that mucous membrane irritation is strong and use is inconvenient; When the second filial generation fertile fancy soap class cleaning supplies are used for the cleaning of individual's skin and hair, also exist and wash the shortcoming that the tight and alkalescence of rear skin injures by force skin; Third generation chemical industry synthetic detergent, its shortcoming is the high pollution of chemical industry building-up process high energy consumption, and there is the harmful toxic matter risk of reacting inevitably side reaction generation in thorough and prior art, synthetic product complex structure is not easily degraded, use rear existence to the secondary pollution of water body, the material such as thickening agent, brightening agent, antiseptic simultaneously in formula is very important social problem for remaining and then affecting health of people of user.
At present, commercially with in cleaning skin and the hair personal cleanliness's articles for use that are main purpose, the pure biosurfactant obtained to utilize microbial fermentation technology is the product of unique functional component, have not been reported.When adding a small amount of rhamnolipid raw material in the detergent compositions proposed in these documents such as China Patent Publication No. CN 102399644 A/CN 103052704 A/CN 104490641 A, need composite wide variety of conventional chemical industry to synthesize class surfactant or enzyme preparation all simultaneously, just cleaning effect can be reached, and normally for membrane module, the washing of fruit and vegerable or textile fabric, do not have to propose the application in personal cleanliness's articles for use field, simultaneously, the well known unfavorable factor of final cleaning product of bringing because rhamnolipid crude extract color and luster and abnormal smells from the patient are not good needs to solve as early as possible, therefore the detergent containing rhamnolipid of these conceptualization is not suitable for the clean of individual body surface grease.
Summary of the invention
For having no pure using rhanolipid as biosurfactant as unique core functional component to prepare the state of the art of personal cleanliness's articles for use at present, and the shortcoming and defect of existing human body washing and skin care product, the present invention is intended to provide a kind of rhamnolipid personal cleanliness articles for use, follow cleaning effect remarkable, simplify formula, stop harmful antiseptic, thickening agent, the minimalist art theory of the adjuvants such as brightening agent, in conjunction with rhamnolipid fermentation technique, the rhamnolipid raw material preparation of producing high-quality is utilized to be applicable to the cleaning supplies of individual, namely be the pure biological preparation type personal cleanliness articles for use of unique decontamination functional component with rhanolipid as biosurfactant, have and clean thoroughly and the feature of safety and Health environmental protection, consumers in general's desires of the people, there is the practicality of reality.
For achieving the above object, the present invention adopts following technical scheme:
By using the biosurfactant plaster shape rhamnolipid of high enrichment after refining through fermented extracted as unique decontamination functional component, take citric acid as pH adjusting agent, take aqua methnae as deodorant tune, be final mastic viscosity modifier with distilled water or deionized water, abundant stirring and evenly mixing after directly adding is adopted to leave standstill degassed preparation personal cleanliness articles for use, according to the convenience of fill and the needs of shelf-life, consider the rhamnolipid content of final products between 50%-90%, the scope of prioritizing selection 70%-80%, the too hard inconvenient fill of mastic and use, mastic is too soft or in flow-like then limited shelf life.According to the difference of condition of storage and use occasion, the optimum formula of personal cleanliness's articles for use is finally obtained by contrast experiment repeatedly, and preparation technology, by repeatedly testing, and test and trying out, to the cleaning effect of human body skin with wash rear skinfeel and all obtain significant technique effect.
Particularly, the invention provides a kind of rhamnolipid personal cleanliness articles for use, counting formula is by weight that rhamnolipid condensed cream 50-95 part of 90%-95%, citric acid 0.1-0.5 part, aqua methnae 0.1-0.5 part, pH value 5.0-10.0, deionized water supplies 100 parts.
Further, count by weight, the invention provides the formula that a kind of rhamnolipid personal cleanliness articles for use preferentially select is, rhamnolipid condensed cream 70-80 part of 90%-95%, citric acid 0.1-0.3 part, aqua methnae 0.2-0.3 part, pH value 6.0-8.0, deionized water supplies 100 parts.
Rhamnolipid personal cleanliness articles for use formula best composition is: count by weight, the rhamnolipid condensed cream of 90%-95% 75 parts, citric acid 0.1 part, aqua methnae 0.2 part, pH value 6.5-7.0 deionized water supplies 100 parts.
In the present invention, the aqua methnae of employing is the water-soluble component of the distillation of fresh natural Herba Menthae plant stem-leaf as deodorant tune, and main component is the pure menthol of fragrance.
The rhamnolipid condensed cream of 90%-95% of the present invention adopts following preparation method to obtain, and by employing fresh plant oil as carbon source, form using Pseudomonas aeruginosa as fermented bacterium through fermenting, extracting, refine and concentrate, concrete steps are as follows:
(1) spawn culture: the P. aeruginosa starter kind of the new purification of scraping one ring, is inoculated in Shake flask medium and cultivates, and concrete condition of culture is, temperature 35-38 DEG C, rotating speed 150-180rpm, cultivates 40-60h and obtains shake-flask seed.
(2) ferment: above-mentioned shake-flask seed is inoculated in fermentation tank, inoculum concentration is the 5-10% of fermentation medium volume, fermentation temperature controls at 35-38 DEG C, without the need to from fermentation liquid bottom ventilation, avoid the technical barrier of later stage foam overflow, more without the need to ethanol froth breaking, mixing speed 20-40rpm, fermentation time is 5-6 days; In sweat, pH can constantly raise, and utilizes the citric acid solution of 55% to be recalled between pH6.5-6.8 by fermentation liquid every 12h, until fermentation ends, obtains yellow green fermentation liquid.
(3) precipitated product: add citric acid in above-mentioned fermentation liquid, adjustment solution ph is 4.0-4.5, leaves standstill 10-30min, abandons supernatant, and collect bottom sedimentation products, obtain rhamnolipid crude product, content is 40-60%.
(4) it is refining: after above-mentioned steps (3) rhamnolipid precipitation crude product is washed away free citric acid to weakly acidic pH with clear water further, redissolve in the soda water of 1-2%, be stirred to thorough dissolving, pH value of solution is adjusted between 7.0-7.5, rhamnolipid concentration 10-20%, solution is heated to 80-90 DEG C, the activated carbon granule adding 3-5% carries out decoloration and deodorization to rhamnolipid crude product, gently rub and stir 1h, leave standstill and treat that nature cools, with the above-mentioned solution of 100 mesh screen, with citric acid, pH value of solution is recalled to 4.0-4.5 again, reclaim sedimentation products, and with clear water washing precipitation 3 times, with soda, precipitation is recalled to pH weakly acidic pH 6.8-7.2, be rhamnolipid condensed cream content by above-mentioned precipitation concentration be 85-95%, above-mentioned rhamnolipid condensed cream is extracted with dehydrated alcohol under-20 DEG C of conditions, extract 3 times, merge above-mentioned alcoholic solution and filter, and after through Rotary Evaporators reclaim ethanol, obtain the refining rhamnolipid condensed cream containing rhamnolipid 90-95%.
In above-mentioned steps (1), described shake-flask seed culture medium is composed as follows: fresh plant oil 10 ~ 20g/L; NaNO
33 ~ 5g/L; K
2hPO
41.5 ~ 2g/L; KH
2pO
40.7 ~ 1g/L; MgSO
40.3 ~ 0.5g/L; Yeast extract 0.1 ~ 0.2g/L, surplus is water.
In above-mentioned steps (2), described fermentation medium is composed as follows: fresh plant oil 25 ~ 35g/L; NaNO
33 ~ 5g/L; K
2hPO
41.5 ~ 2g/L; KH
2pO
40.7 ~ 1g/L; MgSO
40.3 ~ 0.5g/L; Yeast extract 0.05 ~ 0.1g/L, surplus is water.
In above-mentioned steps (5) (6), described fresh plant oil can be Oleum Arachidis hypogaeae semen, walnut oil, olive oil, almond oil, Oleum sesami, soybean oil, Oleum Brassicae campestris, Oleum Helianthi, Fructus Maydis oil, the Semen Flos Carthami wet goods of fresh squeezing, but is not limited to above-mentioned Vegetable oil lipoprotein.
In above-mentioned steps (2), described fermentation tank is not conventional upright gas-lifting type stirred fermentor, but adopt non-perpendicular inclination tank body, and drive tank body to rotate by tank base motor, tank base is provided with fixed paddle, and add the customary secondary equipment such as probe and corresponding control appliance such as including but not limited to temperature common on fermentation tank, pH, dissolved oxygen and liquid level, therefore can realize the stirring ventilation under slow-speed of revolution condition and sufficient dissolved oxygen effect, save the cost of power consumption.
The Pseudomonas aeruginosa that the present invention adopts obtains by buying AB93066 from CCTCC, and those of ordinary skill in the art can be bought by public's approach and obtain.
Rhamnolipid of the present invention is be the concentrated mastic of 90%-95% containing rhamnolipid concentration, for substrate obtains rhamnolipid product through Pseudomonas aeruginosa microbe conversion with fresh squeezing vegetable oil, the structure of rhamnolipid product is that main multiple rhamnolipid homologue forms by monosaccharide fat and two glycolipids, its common feature all has hydrophilic rhamanopyranosyl and lipophilic hydroxyl certain herbaceous plants with big flowers acid groups, rhamnolipid product pH after Hydrolysis kinetics is concentrated is 6.5-7.5 weakly acidic pH, according to the difference of bleaching level, outward appearance presents light red to faint yellow, texture pure as Fresh Honey, special fermentative smell is presented to almost tasteless according to the different odor of deodorize degree, after moistening both hands, rhamnolipid mastic is applied in the centre of the palm, there is after kneading open very fine and smooth white foam, go greasy effect totally thorough, foam is easy to rinse, the greasy feeling of noresidue.
Meanwhile, the present invention specifically provides the preparation method of above-mentioned rhamnolipid personal cleanliness articles for use, and rhamnolipid personal cleanliness articles for use component is according to weight part ratio meter, and concrete preparation process is as follows:
(1) bacterium numbering is adopted to be that the Pseudomonas aeruginosa of AB93066 is cultivated through above-mentioned non-perpendicular ferment tank fresh plant oil is converted into rhamnolipid, go forward side by side onestep extraction, refining, concentrated after the rhamnolipid content that obtains be the rhamnolipid condensed cream of 90-95%, according to weight part ratio meter, it is to be diluted that the rhamnolipid of accurate weighing 70-80 part concentrates mastic.
(2) accurately take 0.1-0.3 part citric acid and 0.2-0.3 part aqua methnae, deionized water supplies 100 parts, and the deionized water solution obtaining 200 grams is for subsequent use.
(3) by the deionized water solution described in step (2), add in the rhamnolipid condensed cream of the 90%-95% described in step (1), be slowly stirred to abundant dissolving, after standing degassed, be product, in product, the content of rhamnolipid is 72-76%, and shelf life of products is not less than 1 year.
Personal cleanliness's articles for use of content of the present invention including but not limited to cleansing milk, shampoo, bath gel, toothpaste etc. with clean skin surface and hair all cleaning agent used.
The compositions that the rhamnolipid that content priority of the present invention uses is monosaccharide fat and two glycolipids, also can be replaced for the compositions of single monosaccharide fat, two glycolipids or its arbitrary proportion that are separated acquisition by technological means.
The aqua methnae that content priority of the present invention uses also can by Oleum menthae known in the art, Flos Rosae Rugosae quintessence oil, Herba Lysimachiae foenum-graeci quintessence oil, etc. natural perfume material replaced.
The water of content of the present invention preferentially use distilled water or deionized water also can by tap water, well water, etc. natural sterilized water replace.
Rhamnolipid is selected directly to prepare rhamnolipid personal cleanliness articles for use as unique decontamination functional component in the present invention, mainly based on the metabolite that rhamnolipid system is transformed through fermentable by edible vegetable oil and fat, character safety is gentle, have no side effect, meet the consumption concept that the mankind pursue safety and Health ecological sustainability, its chemical constitution is simple, is easy to degraded, belongs to environmentally friendly machine; Two is abilities that rhamnolipid has significant moistening and emulsified fat, has excellent emulsification, can meet the needs of cleaning skin of human body and hair dirt completely to all kinds of Vegetable oil lipoprotein and animal oil; Three are rhamnolipids is pure biological preparation, production process is gentle, avoid the high energy consumption high pollution problem that High Temperature High Pressure catalyst in chemical industry synthetic detergent production process brings, environment is more friendly, and has thoroughly stopped the technical barrier that dioxanes class harmful substance that side reaction brings exceeds standard.As everyone knows, when utilizing microbial fermentation technology to obtain its metabolite, the purifying technology of suitable strain, fermentation condition and advanced person is the necessary technology basis obtaining high-quality metabolite, in production practices for years, we have accumulated and have summed up the technology of a whole set of exclusive fermentation for rhamnolipid, and the quality for final products provides abundant and solid technical guarantee.
By implementing concrete summary of the invention of the present invention, following technique effect can be reached.
(1) rhamnolipid personal cleanliness articles for use provided by the invention, utilize the rhamnolipid of safe without toxic side effect can dirt effectively on cleaning skin of human body and hair, especially the grease that direct clear water is washed off is difficult to, as the core decontamination functional component of personal cleanliness's articles for use of the present invention, instead of the use of chemical industry synthesis class detergent completely, while guarantee cleaning effect, avoid all kinds of chemical detergent human body to the damage of skin and hair and stimulation.
(2) rhamnolipid personal cleanliness articles for use provided by the invention, there is remarkable all kinds of Vegetable oil lipoprotein of emulsifying and the effect of animal oil, cleaning effect is thoroughly had to body metabolism sebum, thoroughly can remove the greasy and dirt be deposited in for a long time in pore, emulsifying is rapid, foam is very easily rinsed well by clear water, washes rear skin not tight.
(3) rhamnolipid personal cleanliness articles for use provided by the invention are using citric acid as acidity-basicity regulator, there is safety, gentle feature, make the Acidity of Aikalinity of product close to the natural acid-base status of skin surface, thus the stimulation reduced skin, and give the skin after cleaning fine and smooth soft and smooth skinfeel.
(4) rhamnolipid personal cleanliness articles for use provided by the invention, adding a small amount of natural perfume material aqua methnae is deodorant tune, under the prerequisite ensureing safety non-toxic, gives the fragrance that product is joyful, and refrigerant sensation, and in use body and mind is happy to make consumer.Avoid industrial essence to endanger the cumulative toxicity of human body simultaneously.
(5) rhamnolipid personal cleanliness articles for use preparation process major part provided by the invention belongs to microorganism brewing process, it is the combination of Technology and art, production process environmental protection, can realize a complete continuable Ecological Industry Chain, have immeasurable social benefit and ecological benefits.
(6) rhamnolipid personal cleanliness articles for use provided by the invention are concentrated type product because of it, achieve the antiseptic principle being similar to Mel, by controlling water content, improve osmotic pressure and reach antibacterial effect, thus thoroughly avoid additionally adding that various antiseptic brings through skin absorption and cumulative toxicity risk.
(7) rhamnolipid personal cleanliness articles for use provided by the invention because of its composition simple, relative in third generation chemical industry synthesis class articles for washing tens kinds at least, the product of tens kinds of chemical compositions at most, greatly reduce the existence in skin allergy source, through up to a hundred person-times try out, do not find the consumer to allergy of the present invention so far.
Detailed description of the invention
, for embodiment, the present invention is described below, but the present invention is not limited to following embodiment.
The raw material adopted is: citric acid, natural perfume material, aqua methnae, deionized water etc.This area can be obtained by prior art or prepare.
All raw and auxiliary materials, reagent and the instrument selected in the present invention, equipment are all well known selecting; but do not limit enforcement of the present invention; other reagent more well known in the art and equipment are all applicable to the enforcement of the following embodiment of the present invention, and the rhamnolipid especially utilizing this area to know routine techniques fermentation standby also falls into protection scope of the present invention.
embodiment one: a kind of preparation of rhamnolipid personal cleanliness articles for use
The preparation method of rhamnolipid personal cleanliness articles for use, rhamnolipid personal cleanliness articles for use component is according to weight part ratio meter, and concrete preparation process is as follows:
(1) bacterium numbering is adopted to be that the Pseudomonas aeruginosa of AB93066 is cultivated through above-mentioned non-perpendicular ferment tank fresh plant oil is converted into rhamnolipid, go forward side by side onestep extraction, refining, concentrated after the rhamnolipid content that obtains be the rhamnolipid condensed cream of 90-95%, according to weight part ratio meter, it is to be diluted that the rhamnolipid of accurate weighing 70-80 part concentrates mastic.
(2) accurately take 0.1-0.3 part citric acid and 0.2-0.3 part aqua methnae, deionized water supplies 100 parts, and the deionized water solution obtaining 200 grams is for subsequent use.
(3) by the deionized water solution described in step (2), add in the rhamnolipid condensed cream of the 90%-95% described in step (1), be slowly stirred to abundant dissolving, refining degassed after be product, in product, the content of rhamnolipid is 72-76%, and shelf life of products is not less than 1 year.
The rhamnolipid condensed cream of above-mentioned 90%-95% adopts following preparation method to obtain, and by employing fresh plant oil as carbon source, form using Pseudomonas aeruginosa as fermented bacterium through fermenting, extracting, refine and concentrate, concrete steps are as follows:
(1) spawn culture: the P. aeruginosa starter kind of the new purification of scraping one ring, is inoculated in Shake flask medium and cultivates, and concrete condition of culture is, temperature 35-38 DEG C, rotating speed 150-180rpm, cultivates 40-60h and obtains shake-flask seed.
(2) ferment: above-mentioned shake-flask seed is inoculated in fermentation tank, inoculum concentration is the 5-10% of fermentation medium volume, fermentation temperature controls at 35-38 DEG C, without the need to from fermentation liquid bottom ventilation, avoid the technical barrier of later stage foam overflow, more without the need to ethanol froth breaking, mixing speed 20-40rpm, fermentation time is 5-6 days.In sweat, pH can constantly raise, and utilizes the citric acid solution of 55% to be recalled between pH6.5-6.8 by fermentation liquid every 12h, until fermentation ends, obtains yellow green fermentation liquid.
(3) precipitated product: add citric acid in above-mentioned fermentation liquid, adjustment solution ph is 4.0-4.5, leaves standstill 10-30min, abandons supernatant, and collect bottom sedimentation products, obtain rhamnolipid crude product, content is 40-60%.
(4) it is refining: after above-mentioned steps (3) rhamnolipid precipitation crude product is washed away free citric acid to weakly acidic pH with clear water further, redissolve in the soda water of 1-2%, be stirred to thorough dissolving, pH value of solution is adjusted between 7.0-7.5, rhamnolipid concentration 10-20%, solution is heated to 80-90 DEG C, the activated carbon granule adding 3-5% carries out decoloration and deodorization to rhamnolipid crude product, gently rub and stir 1h, leave standstill and treat that nature cools, with the above-mentioned solution of 100 mesh screen, with citric acid, pH value of solution is recalled to 4.0-4.5 again, reclaim sedimentation products, and with clear water washing precipitation 3 times, with sodium bicarbonate, precipitation is recalled to pH weakly acidic pH 6.8-7.2, be rhamnolipid condensed cream content by above-mentioned precipitation concentration be 85-95%, above-mentioned rhamnolipid condensed cream is extracted with dehydrated alcohol under-20 DEG C of conditions, extract 3 times, merge above-mentioned alcoholic solution and filter, then nearly Rotary Evaporators reclaims ethanol, obtain the refining rhamnolipid condensed cream containing rhamnolipid 90-95%.
In above-mentioned steps (1), described shake-flask seed culture medium is composed as follows: fresh plant oil 10 ~ 20g/L; NaNO
33 ~ 5g/L; K
2hPO
41.5 ~ 2g/L; KH
2pO
40.7 ~ 1g/L; MgSO
40.3 ~ 0.5g/L; Yeast extract 0.1 ~ 0.2g/L, surplus is water.
In above-mentioned steps (2), described fermentation medium is composed as follows: fresh plant oil 25 ~ 35g/L; NaNO
33 ~ 5g/L; K
2hPO
41.5 ~ 2g/L; KH
2pO
40.7 ~ 1g/L; MgSO
40.3 ~ 0.5g/L; Yeast extract 0.05 ~ 0.1g/L, surplus is water.
In above-mentioned steps (5) (6), described fresh plant oil can be Oleum Arachidis hypogaeae semen, walnut oil, olive oil, almond oil, Oleum sesami, soybean oil, Oleum Brassicae campestris, Oleum Helianthi, Fructus Maydis oil, the Semen Flos Carthami wet goods of fresh squeezing, but is not limited to above-mentioned Vegetable oil lipoprotein.
In above-mentioned steps (2), described fermentation tank is not conventional upright gas-lifting type stirred fermentor, but adopt non-perpendicular inclination tank body, and drive tank body to rotate by tank base motor, tank base is provided with fixed paddle, and add the customary secondary equipment such as probe and corresponding control appliance such as including but not limited to temperature common on fermentation tank, pH, dissolved oxygen and liquid level, therefore can realize the stirring ventilation under slow-speed of revolution condition and sufficient dissolved oxygen effect, save the cost of power consumption.
embodiment two: a kind of formula of rhamnolipid personal cleanliness articles for use
The invention provides a kind of rhamnolipid personal cleanliness articles for use, counting formula is by weight that rhamnolipid condensed cream 50-95 part of 90%-95%, citric acid 0.1-0.5 part, aqua methnae 0.1-0.5 part, pH value 5.0-10.0, deionized water supplies 100 parts.
Further, count by weight, the invention provides the formula that a kind of rhamnolipid personal cleanliness articles for use preferentially select is, rhamnolipid condensed cream 70-80 part of 90%-95%, citric acid 0.1-0.3 part, aqua methnae 0.2-0.3 part, pH value 6.0-8.0, deionized water supplies 100 parts.
Rhamnolipid personal cleanliness articles for use formula best composition is: count by weight, the rhamnolipid condensed cream of 90%-95% 75 parts, citric acid 0.1 part, aqua methnae 0.2 part, pH value 6.5-7.0 deionized water supplies 100 parts.
embodiment three: a kind of formula of rhamnolipid personal cleanliness articles for use
Count by weight, rhamnolipid personal cleanliness articles for use formula is, rhamnolipid condensed cream 1000 grams, citric acid 2 grams, aqua methnae 2 grams, deionized water 196 grams, 2 grams of citric acids and 2 grams of aqua methnae are dissolved in after in 196 grams of water, above-mentioned mixed liquor is being joined in 1000 grams of rhamnolipid condensed creams, leaving standstill degassed or centrifugal degassed after abundant stirring and dissolving, detecting qualified rear i.e. finished product.
Further, count by weight, rhamnolipid personal cleanliness articles for use formula is, rhamnolipid condensed cream 500 grams, citric acid 2 grams, aqua methnae 2 grams, 2 grams of citric acids and 2 grams of aqua methnae are dissolved in after in 196 grams of water, are being joined by above-mentioned mixed liquor in 500 grams of rhamnolipid condensed creams by deionized water 196 grams, leave standstill degassed or centrifugal degassed after abundant stirring and dissolving, detect qualified rear i.e. finished product.
embodiment four: a kind of formula of rhamnolipid personal cleanliness articles for use
Antibacterial peptide hand sanitizer formula best composition is: count by weight, rhamnolipid personal cleanliness articles for use formula is, rhamnolipid condensed cream 800 grams, citric acid 1 gram, aqua methnae 2 grams, deionized water 197 grams, 1 gram of citric acid and 2 grams of aqua methnae are dissolved in after in 197 grams of water, above-mentioned mixed liquor is being joined in 800 grams of rhamnolipid condensed creams, leaving standstill degassed or centrifugal degassed after abundant stirring and dissolving, detecting qualified rear i.e. finished product.
The rhamnolipid personal cleanliness articles for use obtained by above-mentioned preparation method are faint yellow thickness paste, transparent pure as Mel, by adopting rhamnolipid to be personal cleanliness's articles for use that unique decontamination functional component is directly prepared, through laboratory emulsification experiment repeatedly verify have thoroughly remove greasy, the effect of deep-pollution-removing, and skinfeel exquisiteness is soft and smooth, clearly pleasant.
embodiment five: the evaluation of rhamnolipid personal cleanliness articles for use clean effect
The rhamnolipid personal cleanliness articles for use liquid prepared by embodiment one, embodiment two carries out washing tests.
(1) the clean effect method of rhamnolipid personal cleanliness articles for use
0.5g rhamnolipid personal cleanliness articles for use are added in beaker, add water to 10g, shake up, drip pure machine oil, vibration, be added drop-wise to till last machine oil do not dissolve, accurate recording rhamnolipid personal cleanliness articles for use dissolve the quality of pure machine oil, characterize the relative size of rhamnolipid personal cleanliness articles for use dirt-removing power by the size of recording quality.
The contrast of table 1-1 detergency ability
The experimental result of the clean effect of rhamnolipid personal cleanliness articles for use shows, known see table 1-1, rhamnolipid personal cleanliness articles for use prepared by the present invention have significant clean effect, clean effect compares Fructus Gleditsiae Abnormalis extract, the cleaning effect of conventional toilet soaps and chemical industry synthesis class compound detergent class cleansing milk improves a lot, and emulsifying is rapid, emulsion is stable lasting, especially suitable skin ingress of oil is more, or having the use of the crowd be accustomed to that makes up, unfavorable crowd is that those bear a pretty, delicate skin and fuel-displaced few infant.
embodiment six: the evaluation of skinfeel after rhamnolipid personal cleanliness articles for use use
There is certain subjectivity in the in-service evaluation of cleaning supplies, we adopt in-service evaluation bonded products nucleus and the construction features more extensively listening to up to a hundred person-times, by arranging multinomial evaluation index, make relatively comprehensively and the evaluation of rationality quantize to give a mark as far as possible, from table 1-2 can find out combination property classic be product of the present invention, and the feature of environmental protection of water can not solve greasy primary demand of deoiling very well, the environmental protection of Fructus Gleditsiae Abnormalis extract and decontamination are all good but lunging property is strong and use procedure trouble.Fancy soap is between natural soap horn extraction and chemical industry synthetic detergent, and chemical industry synthesis class detergent exists the limited shortcoming of complicated component, difficult degradation, detergency.
Sensory evaluation contrast after table 1-2 uses
Be the basic speciality of human nature to the pursuit of safety, environmental protection and health, more classical product is simpler, and therefore product of the present invention will have unlimited vitality in following personal cleanliness's articles for use field, becomes a necessary in mankind's daily life.
Such as same Flos Elaeagni Angustifoliae fragrance has people to think fragrant but also someone thinks smelly, same color has people to like also having people not like, therefore in the invention described above product odour and color and luster two, the evaluation of consumer is passed different judgements on, we can by improve constantly production technology level make the color and luster of product and abnormal smells from the patient more perfect, for consumer provide can not be substituted safety, environmental protection, cleaning, comfortable Consumer's Experience.
Visible in sum, the object of the present invention is to provide a kind of pure biological preparation rhamnolipid personal cleanliness articles for use, with the core functional component that the rhanolipid as biosurfactant had no side effect is unique, be aided with micro-aqua methnae perfumery process, technological means such as utilizing fermentation, extract, be refining, concentrated prepares personal cleanliness's articles for use.Personal cleanliness's articles for use of preparation can be used for the clean decontamination of skin surface and hair, cleaning effect is obvious, thorough removal is greasy, can be used for the clean makeup removing of oil-based cosmetic preparation simultaneously, improve makeup removal efficiency, and there is certain bacteriostasis, because formula is very simple so the risk of skin allergy is dropped to minimum, structure is simple, easily degrade, Environmental compatibility is good, wash rear skinfeel clean clearly soft, not tight, non-scale, be that a new generation is simple, green, environmental protection, health personal cleanliness's articles for use, in personal cleanliness's articles for use field, there is the advantage that can not be substituted.
The present invention is all follow concentrated and simple composition spirit the little change be familiar with by this area and modification all fall into protection scope of the present invention; such as include but not limited to the above-mentioned various changes that can replace classical formula; all fall into protection scope of the present invention; such as include but not limited to by adding a small amount of pigment to change product colour outward appearance; or add the functional component of a small amount of so-called plant essence; all behaviors of disguising replacement of concept all are considered as malice and encroach on the present invention, therefore include but not limited to that above-mentioned behavior all falls into protection scope of the present invention.
Claims (8)
1. rhamnolipid personal cleanliness articles for use, is characterized in that, count formula to be by weight, in rhamnolipid personal cleanliness articles for use component, rhamnolipid condensed cream 50-95 part of 90%-95%, citric acid 0.1-0.5 part, aqua methnae 0.1-0.5 part, pH value 5.0-10.0, deionized water supplies 100 parts.
2. rhamnolipid personal cleanliness articles for use as claimed in claim 1, it is characterized in that, count by weight, in rhamnolipid personal cleanliness articles for use component, rhamnolipid condensed cream 70-80 part of 90%-95%, citric acid 0.1-0.3 part, aqua methnae 0.2-0.3 part, pH value 6.0-8.0, deionized water supplies 100 parts.
3. rhamnolipid personal cleanliness articles for use as claimed in claim 1, is characterized in that, count by weight, in rhamnolipid personal cleanliness articles for use component, the rhamnolipid condensed cream of 90%-95% 75 parts, citric acid 0.1 part, aqua methnae 0.2 part, pH value 6.5-7.0 deionized water supplies 100 parts.
4. a preparation method for the rhamnolipid personal cleanliness articles for use as described in claims 1 to 3 any one, is characterized in that, rhamnolipid personal cleanliness articles for use component is according to weight part ratio meter, and concrete preparation process is as follows:
(1) adopt strain Pseudomonas aeruginosa to cultivate through non-perpendicular ferment tank and fresh plant oil is converted into rhamnolipid, go forward side by side onestep extraction, refining, concentrated after the rhamnolipid content that obtains be the rhamnolipid condensed cream of 90-95%, according to weight part ratio meter, it is to be diluted that the rhamnolipid of accurate weighing 70-80 part concentrates mastic;
(2) accurately take 0.1-0.3 part citric acid and 0.2-0.3 part aqua methnae, deionized water supplies 100 parts, and the deionized water solution obtaining 200 grams is for subsequent use;
(3) by the deionized water solution described in step (2), add in the rhamnolipid condensed cream of the 90%-95% described in step (1), be slowly stirred to abundant dissolving, refining degassed after be product, in product, the content of rhamnolipid is 72-76%, and shelf life of products is not less than 1 year.
5. the preparation method of rhamnolipid personal cleanliness articles for use as claimed in claim 4, the rhamnolipid condensed cream of described 90%-95% adopts following preparation method to obtain, by adopting fresh plant oil as carbon source, form using Pseudomonas aeruginosa as fermented bacterium through fermenting, extracting, refine and concentrate, it is characterized in that, concrete steps are as follows:
(1) spawn culture: the P. aeruginosa starter kind of the new purification of scraping one ring, is inoculated in Shake flask medium and cultivates, and concrete condition of culture is, temperature 35-38 DEG C, rotating speed 150-180rpm, cultivates 40-60h and obtains shake-flask seed;
(2) ferment: be inoculated in fermentation tank by above-mentioned shake-flask seed, inoculum concentration is the 5-10% of fermentation medium volume, and fermentation temperature controls at 35-38 DEG C, without the need to from fermentation liquid bottom ventilation, avoid the technical barrier of later stage foam overflow, more without the need to ethanol froth breaking, mixing speed 20-40rpm, fermentation time is 5-6 days, in sweat, pH can constantly raise, and utilizes the citric acid solution of 55% to recall between pH6.5-6.8 by fermentation liquid every 12h, until fermentation ends, obtain yellow green fermentation liquid;
(3) precipitated product: add citric acid in above-mentioned fermentation liquid, adjustment solution ph is 4.0-4.5, leaves standstill 10-30min, abandons supernatant, and collect bottom sedimentation products, obtain rhamnolipid crude product, content is 40-60%;
(4) it is refining: after above-mentioned steps (3) rhamnolipid precipitation crude product is washed away free citric acid to weakly acidic pH with clear water further, redissolve in the soda water of 1-2%, be stirred to thorough dissolving, pH value of solution is adjusted between 7.0-7.5, rhamnolipid concentration 10-20%, solution is heated to 80-90 DEG C, the activated carbon granule adding 3-5% carries out decoloration and deodorization to rhamnolipid crude product, gently rub and stir 1h, leave standstill and treat that nature cools, with the above-mentioned solution of 100 mesh screen, with citric acid, pH value of solution is recalled to 4.0-4.5 again, reclaim sedimentation products, and with clear water washing precipitation 3 times, with sodium bicarbonate, precipitation is recalled to pH weakly acidic pH 6.8-7.2, be rhamnolipid condensed cream content by above-mentioned precipitation concentration be 85-95%, above-mentioned rhamnolipid condensed cream is extracted with dehydrated alcohol under-20 DEG C of conditions, extract 3 times, merge above-mentioned alcoholic solution and filter, then nearly Rotary Evaporators reclaims ethanol, obtain the refining rhamnolipid condensed cream containing rhamnolipid 90-95%.
6. the preparation method of rhamnolipid personal cleanliness articles for use as claimed in claim 4, it is characterized in that, described shake-flask seed culture medium is composed as follows: fresh plant oil is 10 ~ 20g/L; NaNO
33 ~ 5g/L; K
2hPO
41.5 ~ 2g/L; KH
2pO
40.7 ~ 1g/L; MgSO
40.3 ~ 0.5g/L; Yeast extract 0.1 ~ 0.2g/L, surplus is water.
7. the preparation method of rhamnolipid personal cleanliness articles for use as claimed in claim 4, it is characterized in that, described fermentation medium is composed as follows: fresh plant oil is
25 ~ 35g/L; NaNO
33 ~ 5g/L; K
2hPO
41.5 ~ 2g/L; KH
2pO
40.7 ~ 1g/L; MgSO
40.3 ~ 0.5g/L; Yeast extract 0.05 ~ 0.1g/L, surplus is water.
8. the preparation method of rhamnolipid personal cleanliness articles for use as claimed in claim 4, it is characterized in that, described non-perpendicular fermentation tank is not conventional upright gas-lifting type stirred fermentor, but adopt non-perpendicular inclination tank body, and driving tank body to rotate by tank base motor, tank base is provided with fixed paddle.
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| CN110742819A (en) * | 2019-12-10 | 2020-02-04 | 广州瑞誉化工科技有限公司 | Amino acid facial cleansing honey |
| CN114456880A (en) * | 2020-11-09 | 2022-05-10 | 万华化学(四川)有限公司 | Rhamnolipid kitchen heavy oil stain cleaning agent and preparation method thereof |
| CN114456880B (en) * | 2020-11-09 | 2023-12-19 | 万华化学(四川)有限公司 | Rhamnolipid kitchen heavy oil stain cleaning agent and preparation method thereof |
| CN112933019A (en) * | 2021-04-06 | 2021-06-11 | 杭州伊瑟奇生物科技有限公司 | Oral care solution based on inhibition of microbial quorum sensing and preparation method thereof |
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