CN104886107A - Compound bactericide for preventing and treating chrysanthemum morifolium leaf blight - Google Patents
Compound bactericide for preventing and treating chrysanthemum morifolium leaf blight Download PDFInfo
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- CN104886107A CN104886107A CN201510298957.3A CN201510298957A CN104886107A CN 104886107 A CN104886107 A CN 104886107A CN 201510298957 A CN201510298957 A CN 201510298957A CN 104886107 A CN104886107 A CN 104886107A
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- carbendazim
- iprodione
- bactericide
- leaf blight
- composite bactericide
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Abstract
The invention discloses a bactericide for preventing and treating chrysanthemum morifolium leaf blight caused by chaetomiun globosum, horsetail fusarium, fusarium oxysporum and trichodermakoningiopsis. Effective components comprise iprodione and carbendazim. The bactericide disclosed by the invention has stable chemical performances; iprodione and carbendazim are mutually dissolved when being mixed and do not cause a chemical reaction; the bactericide has wide application, remarkable synergism and safe utilization; the bactericide has very good bacteriostatic activity on various fungi which cause the chrysanthemum morifolium leaf blight in fields at present, and the bacteriostatic activity is obviously higher than iprodione or carbendazim; and iprodione and carbendazim are compounded to realize the synergistic effect, and the drug resistance on the single dosage in the formula by pathogenic bacteria can be delayed, and the bactericide has very important meanings on resistance treatment.
Description
Technical field
The invention belongs to Plant Protection field, relating to a kind of for preventing and treating by chaetomium globosum (Chaetomium globosum), Fusarium equiseti (Fusarium equiseti), Fusarium oxysporum (F.oxysporum) and the composite bactericide intending the FLOS CHRYSANTHEMI ALBA from Haizhou of China leaf blight that healthy and free from worry wood mould (Trichoderma koningiopsis) causes particularly.
Technical background
Traditional Chinese medicine is the basis of Development of Traditional Chinese Medicine.Chinese Medicine Industry based on medicinal plant be our province to weigh industry, one of industry of Ye Shi China minority most international comparison advantage.Zhejiang Province's natural resources of Chinese medicinal materials is abundant, various in style, and have Chinese material medicine resource more than 2000 and plant, wherein medicinal plant kind is more than kind more than 1700, reserves about more than 10 hundred million kilograms.In long-term production practices, a collection of high-quality medicinal material with Zhejiang characteristic that to define with " eight Zhe's " be representative, occupies critical role in national medicinal material market, with the obvious advantage.
FLOS CHRYSANTHEMI ALBA from Haizhou of China is as the traditional medicine-food two-purpose cultivated plant of China, and be one of Zhejiang Province eight your name medicinal material " eight Zhe's ", the sweet heat-clearing of taste, is commonly used to flat liver improving eyesight, clearing heat and detoxicating.Main product is in Tongxiang City, Zhejiang Province, and area under cultivation more than 60,000 mu, produces nearly 5000 tons of dried flower per year, the average year output value more than 1.8 hundred million.
The safe, effective, stable, controlled of Chinese medicine is the key realizing the modernization of Chinese medicine.This just requires that specification cultivation carried out by traditional Chinese medicine, and the sport technique segment that disease screening and improvement are the weakest in this process exactly.Particularly along with the expansion of more and more medicinal plant cultivation scale, the disease problem that threatens becomes increasingly conspicuous, and the production of the administrable such as new expression, drug resistance problems plant brings serious threat.Make genunie medicinal materials originally lose the market competitiveness gradually, and then cause whole Zhejiang area to face the difficult situations such as Chinese Medicinal Materials Markets share shrink, even jeopardize China's medicinal plant and produce healthy and sustainable development and traditional Chinese medicine foreign exchange earning.
Research finds, the FLOS CHRYSANTHEMI ALBA from Haizhou of China leaf blight of Zhejiang area is mainly infected by disease funguses such as chaetomium globosum (Chaetomium globosum), Fusarium equiseti (Fusarium equiseti), Fusarium oxysporum (F.oxysporum) and the healthy and free from worry wood of plan mould (Trichoderma koningiopsis) and causes in recent years.Owing to producing the upper bactericide lacking treatment medicinal plant diseases, therefore mainly rely on field crop bactericide and prevent and treat.Add long-term single drug, resistance problems is more and more serious, and urgent need is found for medicinal plant diseases and had the replacement medicament of good fungistatic effect.
Dicarboximide fungicide (as iprodione), is a class high-efficiency broad spectrum, contact killing type bactericide, has certain local therapeutic effects, has been widely used in multiple monoid plant pathogenic fungi, resistance problem also time have report.
The action character of benzimidazole germicide (as carbendazim) does not suppress spore germination, but significantly can suppress the growth of germ tube elongation and mycelia at low concentrations, and fungicidal spectrum is wide, safer to mammal.But because this kind of pharmacy effect target is single, pathogen is easy to drug-resistant mutation occurs, and belongs to typical resistance risk medicament.Due to the reason such as low price, use habit, be the current the most widely used a kind of bactericide of China, resistance problem happens occasionally.
Summary of the invention
The object of the invention is for the deficiencies in the prior art, provide a kind of bactericide preventing and treating FLOS CHRYSANTHEMI ALBA from Haizhou of China leaf blight, can be used for medicinal plant diseases preventing and controlling, solve the problem that pathogen easily develops immunity to drugs to existing bactericide or existing bactericide single dose drug effect is not strong.
The present invention prevents and treats the bactericide of FLOS CHRYSANTHEMI ALBA from Haizhou of China leaf blight, and active ingredient comprises iprodione, carbendazim;
As preferably, described iprodione and the weight ratio of carbendazim are 1:10 ~ 10:1, are more preferably 1:1;
The weight concentration of described iprodione is 48 ﹪ ~ 95 ﹪, and the weight concentration of carbendazim is 0.1 ﹪ ~ 1.8 ﹪;
The bactericide that the present invention prevents and treats FLOS CHRYSANTHEMI ALBA from Haizhou of China leaf blight is the conventional formulations of pesticide, and in preparation, the mass content of active ingredient is 50 ﹪ ~ 95 ﹪; All the other are conven-tional adjuvants, filler;
It is as follows to the drug-fast mechanism that produces of single dose in formula that the present invention adopts compound disinfectant can delay pathogen:
Dicarboximide fungicide mainly acts on pathogen bi-component histidine kinase (TCHK) signal pathway, by suppressing pathogen protein kinase, controls the Intracellular signals of many cell functions, thus plays the effect of interference cell growth.Benzimidazole germicide is mainly combined with germ 'beta '-tubulin, thus stops cell to carry out mitosis, reaches the object suppressing pathogen growth.Two series bactericidal agent mechanism of action are different, can delay the resistance problems that pathogen produces single dose.
Bactericide stable chemical nature of the present invention, during mixing, iprodione and carbendazim two kinds of compositions dissolve each other, chemical reaction does not occur, of many uses, synergy is remarkable, use safety, all kinds of fungies current field being caused to FLOS CHRYSANTHEMI ALBA from Haizhou of China leaf blight have good bacteriostatic activity, and apparently higher than iprodione or carbendazim single dose, namely both are composite synergistic effect, and pathogen can be delayed to the pesticide resistance that produces of single dose in formula, resistance management is had great importance.
The present invention is through indoor bioassay, and built agent effectively can suppress the growth causing the fungal disease chaetomium globosum of FLOS CHRYSANTHEMI ALBA from Haizhou of China leaf blight, Fusarium equiseti, Fusarium oxysporum and the healthy and free from worry wood of plan mould, thus plays the effect of disease control.
The present invention shows through field control effectiveness test, and after built agent process, FLOS CHRYSANTHEMI ALBA from Haizhou of China per mu yield dried flower amount significantly improves, and can play the effect of disease-preventing and yield-increasing.
Accompanying drawing explanation
Fig. 1 is the action effect figure of different ratio bactericide to chaetomium globosum F13N-1b;
Fig. 2 is the action effect figure of different ratio bactericide to Fusarium equiseti F13P-2;
Fig. 3 is the action effect figure of different ratio bactericide to Fusarium oxysporum Fo 006;
Fig. 4 is that different ratio bactericide is to the action effect figure intending the mould F13N-2 of healthy and free from worry wood.
Embodiment
Below in conjunction with specific embodiment, the present invention is further analyzed.
The preparation of embodiment 1,48 ﹪ iprodione+1.8 ﹪ Bavistin microemulsion:
Iprodione, carbendazim and acetone are put into container, stirring and dissolving, make oil phase; Agriculture breast 33#, Nongru-700 # are put into another container stirring and dissolving with water and becomes aqueous phase; Oil phase is added in aqueous phase, in a mixer strong agitation, namely obtain microemulsion.
The preparation of embodiment 2,83 ﹪ iprodione+1 ﹪ carbendazol wettable powder:
By recipe requirements, former medicine, various auxiliary agent and filler are fully mixed, pulverize through airslide disintegrating mill, until grain fineness reaches standard value (5 μm once), namely obtain wetting powder.
The preparation of embodiment 3,95 ﹪ iprodione+0.1 ﹪ carbendazol wettable powder
By recipe requirements, former medicine, various auxiliary agent and filler are fully mixed, pulverize through airslide disintegrating mill, until grain fineness reaches standard value (less than 5 μm), namely obtain wetting powder.
Embodiment 4, built agent are to the dull and stereotyped test of pesticide effectiveness causing 4 of FLOS CHRYSANTHEMI ALBA from Haizhou of China leaf blight kinds of disease funguses
Iprodione and carbendazim active compound are dissolved in acetone respectively, are then mixed in medium by the Different Weight ratio of iprodione and carbendazim, measure the inhibit activities to disease fungus growth.Iprodione and carbendazim weight ratio are respectively 1:10,1:5,1:1,5:1 and 10:1, the weight concentration of described iprodione is 48 ﹪ ~ 95 ﹪, the weight concentration of carbendazim is 0.1 ﹪ ~ 1.8 ﹪, and after dilution, actual drug concentration is respectively 0.91mg/ml:18.18 μ g/ml, 1.67mg/ml:16.67 μ g/ml, 5mg/ml:10 μ g/ml, 16.67mg/ml:1.67 μ g/ml and 18.18mg/ml:0.91 μ g/ml.This test repetition 3 times.
The test strain adopted is respectively chaetomium globosum F13N-1, Fusarium equiseti F13P-2, Fusarium oxysporum Fo006 and intends the mould F13N-2 of healthy and free from worry wood, and these bacterial strains all gather on the FLOS CHRYSANTHEMI ALBA from Haizhou of China plant of spontaneous leave rot.
Measure the average strain growth diameter under pharmacy effect, use software SigmaPlot 10 to calculate pharmacy effect effect.
Chemicals treatment effect is shown in Fig. 1-4.Using composite ratio as abscissa, wherein 1 represent iprodione single dose; 2 represent carbendazim single dose, and 3 represent iprodione and carbendazim ratio is 1:10; 4 represent iprodione and carbendazim ratio is 1:5; 5 represent iprodione and carbendazim ratio is 1:1; 6 represent iprodione and carbendazim ratio is 5:1; 7 represent iprodione and carbendazim ratio is 10:1.With average colony diameter for ordinate, unit is cm.
The conk diameter mean value that the pharmacy type adopted when each pillar represents independent mensuration in figure is corresponding, can obviously find out when built agent ratio 1:1, and the suppression that colony growth is subject to is the most obvious, and namely drug effect is best.
Embodiment 5, field control effectiveness test
This test is carried out in FLOS CHRYSANTHEMI ALBA from Haizhou of China planting base, crossdrift town, Tongxiang City.Every block testing ground area is 1 mu, subjects is the FLOS CHRYSANTHEMI ALBA from Haizhou of China plant that upgrowth situation is identical, process with composite bactericide provided by the invention (iprodione and carbendazim weight ratio are 1:1, and medicine final concentration is respectively 5mg/ml and 5 μ g/ml after dilution), iprodione single dose (medicine final concentration 10mg/ml), carbendazim single dose (medicine final concentration is 20 μ g/ml) and clear water respectively.FLOS CHRYSANTHEMI ALBA from Haizhou of China was used before flowering stage, every control in 10 days once, sprayed 2 times continuously, repeated 3 times, coprocessing FLOS CHRYSANTHEMI ALBA from Haizhou of China 12 mu, add up output and carry out Toxicity Analysis after FLOS CHRYSANTHEMI ALBA from Haizhou of China is plucked.
Preventive effect result (with FLOS CHRYSANTHEMI ALBA from Haizhou of China output for index) is in table 1.In same treatment situation, with FLOS CHRYSANTHEMI ALBA from Haizhou of China per mu yield dried flower amount for index, after using built agent, output is the highest, best to FLOS CHRYSANTHEMI ALBA from Haizhou of China leaf blight preventive effect.
The effect of table 1 built agent control FLOS CHRYSANTHEMI ALBA from Haizhou of China leaf blight
Above-described embodiment is not that the present invention is not limited only to above-described embodiment for restriction of the present invention, as long as meet application claims, all belongs to protection scope of the present invention.
Claims (7)
1. a composite bactericide, is characterized in that: active ingredient comprises iprodione, carbendazim.
2. composite bactericide according to claim 1, is characterized in that: described iprodione and the weight ratio of carbendazim are 1:10 ~ 10:1.
3. composite bactericide according to claim 2, is characterized in that: described iprodione and the weight ratio of carbendazim are 1:1.
4. composite bactericide according to claim 1, is characterized in that: the weight concentration of described iprodione is 48 ﹪ ~ 95 ﹪, and the weight concentration of carbendazim is 0.1 ﹪ ~ 1.8 ﹪.
5. composite bactericide according to claim 1, is characterized in that: composite bactericide is the conventional formulations of pesticide, and in preparation, the mass content of active ingredient is 50 ﹪ ~ 95 ﹪, and all the other are conven-tional adjuvants, filler.
6. composite bactericide according to claim 1, is characterized in that: for preventing and treating FLOS CHRYSANTHEMI ALBA from Haizhou of China leaf blight.
7. composite bactericide according to claim 6, is characterized in that: the FLOS CHRYSANTHEMI ALBA from Haizhou of China leaf blight caused by chaetomium globosum (Chaetomium globosum), Fusarium equiseti (Fusarium equiseti), Fusarium oxysporum (F.oxysporum) and the healthy and free from worry wood of plan mould (Trichoderma koningiopsis) for control.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201510298957.3A CN104886107A (en) | 2015-06-03 | 2015-06-03 | Compound bactericide for preventing and treating chrysanthemum morifolium leaf blight |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510298957.3A CN104886107A (en) | 2015-06-03 | 2015-06-03 | Compound bactericide for preventing and treating chrysanthemum morifolium leaf blight |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107164233A (en) * | 2016-12-28 | 2017-09-15 | 贵州省植物保护研究所 | A kind of chaetomium globosum LJ S2L1 bacterial strains and its application |
| CN107475123A (en) * | 2017-08-16 | 2017-12-15 | 中国人民解放军第二军医大学 | Roxburgh anoectochilus terminal bud endogenetic fungus and its application |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4044145A (en) * | 1974-07-15 | 1977-08-23 | Rhone-Poulenc Industries | Fungicidal compositions |
-
2015
- 2015-06-03 CN CN201510298957.3A patent/CN104886107A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4044145A (en) * | 1974-07-15 | 1977-08-23 | Rhone-Poulenc Industries | Fungicidal compositions |
Non-Patent Citations (1)
| Title |
|---|
| 无: "等级证号：PD20120391", 《中国农药信息网》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107164233A (en) * | 2016-12-28 | 2017-09-15 | 贵州省植物保护研究所 | A kind of chaetomium globosum LJ S2L1 bacterial strains and its application |
| CN107475123A (en) * | 2017-08-16 | 2017-12-15 | 中国人民解放军第二军医大学 | Roxburgh anoectochilus terminal bud endogenetic fungus and its application |
| CN107475123B (en) * | 2017-08-16 | 2020-02-11 | 中国人民解放军第二军医大学 | Anoectochilus roxburghii endophytic fungus and application thereof |
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Application publication date: 20150909 |