CN104865350A - Insecticidal toxicity evaluation method of green muscadine fungus and chlorantraniliprole combined utilization - Google Patents
Insecticidal toxicity evaluation method of green muscadine fungus and chlorantraniliprole combined utilization Download PDFInfo
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Abstract
The invention discloses an insecticidal toxicity evaluation method of green muscadine fungus and chlorantraniliprole combined utilization, and provides a method for identifying insecticidal efficacy of chemical agent and fungicide combined utilization. The method for identifying the insecticidal efficacy of the chemical agent and the fungicide combined utilization includes the following steps: 1) respectively detecting LC50 values of the chemical agent, the fungicide, and mixture composed of the chemical agent and the fungicide; 2) converting a bacteria agent concentration unit into a chemical agent concentration unit; 3) calculating a co-toxicity coefficient of the chemical agent and fungicide combined utilization, wherein if the co-toxicity coefficient is equal or greater than 100, the insecticidal efficacy of the chemical agent and fungicide combined utilization is equal or greater than an independent effect of the chemical agent or the fungicide, and a synergized effect is achieved, and if the co-toxicity coefficient is less than 100, the insecticidal efficacy of the chemical agent and the fungicide combined utilization is less than the independent effect, and an antagonistic effect is caused. By adopting the method for identifying the insecticidal efficacy of the chemical agent and the fungicide combined utilization, green muscadine fungus bacterial strain, the chlorantraniliprole and migratory locust are used as experimental materials, an uniform unit is used to convert the LC50 values of the green muscadine fungus and the chlorantraniliprole, and then the co-toxicity coefficient is calculated, and an evaluation criterion is set.
Description
Technical field
The present invention relates to biological technical field, particularly relate to the insecticidal toxicity evaluation method of a kind of green muscardine fungus and Rynaxypyr conbined usage.
Background technology
Green muscardine fungus is the important insect endogenetic fungus of a class, can parasitic various insects.Green muscardine fungus comprises adhesion, spore germination to host's infection processs, penetrate in polypide, body and to grow and lethal, usual 24 hours, and green muscardine fungus can penetrate insect body wall rapidly, invades in pin main body, insect just can be made lethal after one week even longer time.According to relevant field test, the green muscardine fungus finish using 2.0E+12 to 1.0E+13 spore/hectare can kill the locust of 80%-98% after 7 to 21 days.But green muscardine fungus is slow to the process of killing of insect, again by the impact of environmental factor, sprayer machine, the quality of the pharmaceutical preparations in practical application, do not reach the preventive effect effect of expection.Therefore there is relevant scholar to be mixed with green muscardine fungus by chemical agent to use to prevent and treat agricultural pests, shorten its action time, and obtain good insecticidal effect.
The research with or without synergy mixed with chemical pesticide of current related Evaluation green muscardine fungus, the chemical agent of the single concentration of most employing (sublethal dose) is mixed with green muscardine fungus, by simply compare green muscardine fungus alone and mixed with chemical pesticide after the size of mortality ratio judge without synergistic effect.But, also lack the standard evaluating green muscardine fungus effect mixed with chemical pesticide at present, in the mixed research of green muscardine fungus with chemical pesticide, more do not occur the method evaluating both synergy effects with co-toxicity coefficient.
Summary of the invention
The object of this invention is to provide a kind of method identifying chemical pesticide and green muscardine fungus coupling insecticidal effect.
Method provided by the invention, comprises the steps:
1) respectively following A, B and C tri-groups of process: A are carried out to worm, chemical pesticide process separately, B, green muscardine fungus process separately, C chemical pesticide and green muscardine fungus coupling aftertreatment;
Plant raising worm after described chemical pesticide is treated to separately and soaks with chemical pesticide solution;
Described green muscardine fungus is treated to separately sprays worm with muscardine spore suspension;
Described chemical pesticide and green muscardine fungus coupling aftertreatment are first spray worm with muscardine spore suspension, obtain the worm after green muscardine fungus process; Plant raising after again the worm after described green muscardine fungus process being soaked with chemical pesticide solution;
2) detect the mortality ratio that described A, B and C tri-groups respectively organizes the rear worm of process, calculate A group LC
50A, B group LC
50Bwith C group LC
50AB; And at calculating LC
50Band LC
50ABtime, described muscardine spore suspension concentration is converted into the concentration consistent with described chemical pesticide solution concentration unit by transfer equation;
Described transfer equation is y=2E-08x, and wherein, x is muscardine spore suspension concentration, and y is the muscardine spore suspension concentration after conversion;
3) by described LC
50Avalue and described LC
50ABco-toxicity coefficient CTC value by following formulae discovery chemical pesticide and green muscardine fungus coupling group:
Wherein, the actual toxicity index of described mixture
Number percent+green muscardine fungus TI × green muscardine fungus that described mixture theory toxicity index TTI=chemical pesticide TI × chemical pesticide accounts for mixture accounts for the number percent of mixture;
Described chemical pesticide TI is 100, and described green muscardine fungus
Or described green muscardine fungus TI is 100, and described chemical pesticide
The number percent that described chemical pesticide accounts for mixture is chemical pesticide solution concentration/(the muscardine spore suspension concentration after chemical pesticide solution concentration+conversion);
The number percent that described green muscardine fungus accounts for mixture is muscardine spore suspension concentration/(the muscardine spore suspension concentration after chemical pesticide solution concentration+conversion) after conversion;
4) according to following standard determination chemical pesticide and green muscardine fungus coupling insecticidal effect: if described co-toxicity coefficient is more than or equal to 100, then the insecticidal effect of described chemical pesticide and green muscardine fungus coupling is synergy; If described co-toxicity coefficient is less than 100, then the insecticidal effect of described chemical pesticide and green muscardine fungus coupling is antagonism;
Described synergy is that chemical pesticide and green muscardine fungus coupling insecticidal effect are more than or equal to the insecticidal effect that chemical pesticide processes separately or green muscardine fungus processes separately;
Described antagonism is that chemical pesticide and green muscardine fungus coupling insecticidal effect are less than the insecticidal effect that described chemical pesticide processes separately or described green muscardine fungus processes separately.
2E-08 in above-mentioned y=2E-08x be science counting literary style, 2E-08 represent 2 be multiplied by 10-8 powers, y=2E-08x also can be written as y=(1 × 10
-8) X.
In said method,
Described LC
50A, LC
50Bwith described LC
50ABnumerical value unit is mg/L.
In said method, described worm is the nymph of a locust; Described chemical pesticide is Rynaxypyr.
In said method, in described Rynaxypyr and green muscardine fungus coupling processed group, described chemical pesticide solution concentration and described change after the mass ratio of muscardine spore suspension concentration be 1:4,1:1 or 4:1.
Experiment of the present invention proves, the present invention proposes a kind of possible ways evaluated fungus insecticide and chemical pesticide conbined usage virulence and evaluate first, and this evaluation method have passed through experimental verification, is provided with evaluation index, provides standard to evaluation.
Accompanying drawing explanation
Fig. 1 is the mortality ratio being used alone Rynaxypyr.
Fig. 2 is the mortality ratio being used alone green muscardine fungus.
Fig. 3 is the mortality ratio of Rynaxypyr and green muscardine fungus 0.2:0.8.
Fig. 4 is the mortality ratio of Rynaxypyr and green muscardine fungus 0.5:0.5.
Fig. 5 is the mortality ratio of Rynaxypyr and green muscardine fungus 0.8:0.2.
Embodiment
Below by specific embodiment, the present invention will be described, but the present invention is not limited thereto.
The experimental technique used in following embodiment if no special instructions, is conventional method.Reagent used in following embodiment, biomaterial etc., if no special instructions, all can obtain from commercial channels.
Green muscardine fungus involved in following embodiment is Metarhizium Strains Metariziumanisoplae MBJQH2-2, and deposit number is: CGMCC No.4275, and it is recorded in Chinese patent mandate text CN 102021122B.
The former medicine of the Rynaxypyr used in following embodiment is the product of Dupont Chemical Group (China) company limited.
The acquisition of the insecticidal toxicity evaluation method of embodiment 1, green muscardine fungus and Rynaxypyr conbined usage
One, Rynaxypyr is to migratory locusts MLC (median lethal concertration) LC
50the mensuration of value
Test with 3 age the migratory locusts nymph of a locust for research object, adopt dipping fourier methods to feed migratory locusts.
The preparation of Rynaxypyr liquid: the mother liquor first former for Rynaxypyr medicinal acetone being mixed with 4mg/mL, then progressively being diluted by mother liquor with sterilized water is 20mg/L, 5mg/L, 0.5mg/L, 0.05mg/L and 0.01mg/L.
Rynaxypyr liquid process migratory locusts method: by fresh free of contamination wheat leaf bundle (3cm diameter), be immersed in the Rynaxypyr liquid of variable concentrations and take out after 10s, by wheat leaf bundle leaching 10s sterilized water in contrast, indoor are dried rear equivalent and are put into Breeding container.By of the same size 3 age the migratory locusts nymph of a locust be positioned over and above-mentioned the Breeding container of wheat after process be housed, each concentration process 45 migratory locusts, experiment multiplicity is 3, raises in insectary by the migratory locusts of process, condition of culture is light application time: interlunation=14h:10h, rearing conditions is 30 ± 2 DEG C.
Data statistical approach: from the 1st day meter after the process wheat 24h that feeds, add up dead borer population in continuous 7 days.Touching polypide with dissecting needle, is dead without significant reaction person.Process the mortality ratio of the 7th day as shown in Figure 1, and adopt SPSS statistical software to calculate the virulence equation of Rynaxypyr to migratory locusts to be: (y is the probit value of mortality ratio to y=4.65+0.51x, x is the lg value of concentration), coefficient R is 0.94, and Rynaxypyr is to the LC of migratory locusts
50=4.76mg/L.
Two, green muscardine fungus is to migratory locusts MLC (median lethal concertration) LC
50mensuration
1, green muscardine fungus suspending liquid process migratory locusts
The configuration of muscardine spore suspending liquid: by the conidium of green muscardine fungus with 0.1% Tween-80 sterilized water be mixed with the suspending liquid that mother liquid concentration is 2.5E+08 spore quantity/mL, be 4 concentration for the treatment of with the Tween-80 sterilized water stepwise dilution of 0.1%, be respectively 2.5E+07,2.5E+06,2.5E+05,2.5E+04 spore quantity/mL.
The method of green muscardine fungus suspending liquid process migratory locusts: select of the same size 3 age the nymph of a locust, adopt spray tower equipment to 3 age the nymph of a locust carry out the process of muscardine spore suspension spray, and be treated to contrast with the Tween-80 sterilized water spraying of 0.1%, 15/mL, each concentration process 45 migratory locusts, repeat 3 times, the migratory locusts processed are placed in insectary, condition of culture is light application time: interlunation=14h:10h, rearing conditions is 30 ± 2 DEG C.
2, LC
50calculating
Data statistical approach: from the 1st day meter after green muscardine fungus spraying process migratory locusts 24h, add up dead borer population in continuous 7 days.Touching polypide with dissecting needle, is dead without significant reaction person.Process the mortality ratio of the 7th day as shown in Figure 2, and adopt SPSS statistical software to calculate the virulence equation of green muscardine fungus to migratory locusts to be: y=5.76+0.93x (y is the probit value of mortality ratio, and x is the lg value of concentration), coefficient R is 0.94.
Calculate LC
50time, the concentration unit of the muscardine spore suspending liquid of above-mentioned 1 is converted to consistent with the concentration unit of Rynaxypyr, transfer equation is that (x is the concentration of green muscardine fungus to y=2E-08x, y is the concentration after transforming), the concentration after conversion is respectively 5mg/L, 0.5mg/L, 0.05mg/L, 0.005mg/L and 0.0005mg/L.
Green muscardine fungus is to the LC of migratory locusts
50=0.15mg/L.
Being calculated by above-mentioned transfer equation with spore quantity/mL is the LC of unit
50=0.75E+07 spore quantity/mL.
Three, Rynaxypyr and the symphyogenetic virulence evaluation of green muscardine fungus
1, Rynaxypyr and green muscardine fungus synergy
Wheat leaf bundle after the Rynaxypyr process of variable concentrations: the mother liquor former for Rynaxypyr medicinal acetone being mixed with 4mg/mL, then being diluted by mother liquor with sterilized water is 5mg/L, is then diluted to corresponding concentration (see table 1) with sterilized water.By fresh free of contamination wheat leaf bundle (3cm diameter), be immersed in the Rynaxypyr liquid of variable concentrations and take out after 10s, indoor are dried rear equivalent and are put into raising basket, and the wheat seedling that the Rynaxypyr liquid obtaining being equipped with variable concentrations soaks raises basket.With wheat leaf bundle leaching 10s sterilized water in contrast.
Variable concentrations muscardine spore suspending liquid: by the conidium of green muscardine fungus with 0.1% Tween-80 sterilized water join suspension mother liquor for concentration 2.5E+08 spore quantity/mL, the Tween-80 sterilized water with 0.1% is diluted to corresponding concentration (table 1).
Rynaxypyr and green muscardine fungus synergy:
Select of the same size 3 age the nymph of a locust, adopt spray tower successively to 3 age the nymph of a locust carry out the process of variable concentrations muscardine spore suspension spray, 15/mL, each concentration process 45 migratory locusts, repeat 3 times, and process migratory locusts for contrast with the Tween-80 sterilized water spraying of 0.1%, be then transferred to by the migratory locusts of process in the raising basket of the wheat seedling of the Rynaxypyr liquid immersion that variable concentrations is housed, concrete concentration for the treatment of is in table 1.
2, LC
50calculate
The migratory locusts processed are placed in insectary, and condition of culture is light application time: interlunation=14h:10h, rearing conditions is 30 ± 2 DEG C.Within continuous 7 days, adding up dead borer population, touch polypide with dissecting needle, is death standard without significant reaction person, processes the mortality ratio of the 7th day as shown in Figure 3-Figure 5.
The hybrid processing concentration of table 1 Rynaxypyr and green muscardine fungus
Data statistical approach: from green muscardine fungus process migratory locusts and cultivate in basket the wheat putting into Rynaxypyr process raise 24h after the 1st day meter, within continuous 7 days, add up dead borer population.Touching polypide with dissecting needle, is dead without significant reaction person.
Calculate LC
50time, the concentration unit of the muscardine spore suspending liquid of above-mentioned 1 is converted to consistent with the concentration unit of Rynaxypyr, transfer equation is y=2E-08x (x is the concentration of green muscardine fungus, and y is the concentration after transforming), and concrete concentration for the treatment of and concentration proportioning are in table 2.
The melting concn of table 2 Rynaxypyr and the green muscardine fungus after changing
Rynaxypyr: green muscardine fungus (mass ratio)=0.2:0.8 hybrid processing mortality ratio of the 7th day as shown in Figure 3, and adopt SPSS statistical software to calculate Rynaxypyr: during green muscardine fungus=0.2:0.8 to the virulence equation of migratory locusts be: y=6.15+0.59x, coefficient R is 0.91, and wherein the concentration of x is mg/l; Obtain the LC of hybrid processing to migratory locusts
50=0.01mg/L.
Rynaxypyr: the concentration of green muscardine fungus (mass ratio)=0.2:0.8 wherein green muscardine fungus is 0.008mg/L, is LC by the concentration of equations turned green muscardine fungus
50=4.0E+05 spore quantity/mL.
Rynaxypyr: green muscardine fungus (mass ratio)=0.5:0.5 hybrid processing mortality ratio of the 7th day as shown in Figure 4, and adopt SPSS statistical software to calculate Rynaxypyr: during green muscardine fungus=0.5:0.5 to the virulence equation of migratory locusts be: y=5.91+0.53x, coefficient R is 0.85, and wherein the concentration of x is mg/l; Obtain the LC of hybrid processing to migratory locusts
50=0.02mg/L.
Rynaxypyr: in green muscardine fungus=0.5:0.5 hybrid processing, the concentration of green muscardine fungus is 0.01mg/L is LC by the concentration of equations turned green muscardine fungus
50=5.0E+05 spore quantity/mL.
Rynaxypyr: green muscardine fungus=0.8:0.2 hybrid processing mortality ratio of the 7th day as shown in Figure 5, and adopt SPSS statistical software to calculate Rynaxypyr: during green muscardine fungus=0.8:0.2 to the virulence equation of migratory locusts be: y=4.76+0.78x, coefficient R is 0.85, and wherein the concentration of x is mg/l; Obtain the LC of hybrid processing to migratory locusts
50=2.01mg/L.
Rynaxypyr: in green muscardine fungus=0.8:0.2 hybrid processing, the concentration of green muscardine fungus is 0.402mg/L is LC by the concentration of equations turned green muscardine fungus
50=2.01E+07 spore quantity/mL.
4, the calculating of coefficient (CTC) is spent after green muscardine fungus and Rynaxypyr synergy together
The calculating of spending coefficient after both synergy together adopts the abundant computing method of Sun Yun and is improved.
The calculating of co-toxicity coefficient:
Theoretical toxicity index (TTI)=TI (A) × medicament A number percent+TI (the B) × medicament B number percent in mixture in mixture of mixture
Medicament A number percent in mixture is concentration/(concentration of the concentration+medicament B of medicament A) of medicament A;
Medicament B number percent in mixture is concentration/(concentration of the concentration+medicament B of medicament A) of medicament B;
Above-mentioned standard agent A and medicament A is Rynaxypyr, and standard agent B and medicament B is green muscardine fungus; Also can be green muscardine fungus by choice criteria medicament A and medicament A, standard agent B and medicament B is Rynaxypyr; That adopt in the present embodiment is the former.
The co-toxicity effect of Rynaxypyr and green muscardine fungus is judged with CTC value; CTC is more than or equal to 100 expression mixtures synergistic effect; CTC is less than 100 expression mixtures antagonism.
Synergistic effect is that the nymph of a locust mortality ratio of mixture desinsection is more than or equal to the nymph of a locust mortality ratio being used alone A medicament or being used alone B medicament; The nymph of a locust mortality ratio of the present mixture of antagonist is less than the nymph of a locust mortality ratio being used alone A medicament or being used alone B medicament.
The application of the insecticidal toxicity evaluation method of embodiment 2, green muscardine fungus and Rynaxypyr conbined usage
1, process
Respectively following A, B and C tri-groups of process are carried out to the nymph of a locust:
A, Rynaxypyr process separately (one of embodiment 1);
B, green muscardine fungus process separately (two of embodiment 1);
C, Rynaxypyr and green muscardine fungus coupling aftertreatment (three of embodiment 1);
2, detect the mortality ratio of worm after each group of process, calculate LC after each group of process
50value
LC after calculating each group of process
50during value, concentration unit used for green muscardine fungus process is converted to the consistent concentration unit of the concentration unit used with Rynaxypyr process by transfer equation;
Transfer equation is y=2E-08x (x is the concentration of green muscardine fungus, and y is the green muscardine fungus concentration after transforming).
Be used alone the LC of Rynaxypyr
50A=4.76mg/L.
Be used alone the LC of green muscardine fungus
50B=0.15mg/L.
The LC of green muscardine fungus and Rynaxypyr conbined usage
50AB=0.01mg/L.
3, the co-toxicity coefficient CTC of green muscardine fungus and Rynaxypyr Combined Treatment is calculated
Utilize the LC of each group
50be worth by embodiment 1 three 4 computing formula calculate the co-toxicity coefficient CTC of green muscardine fungus and Rynaxypyr Combined Treatment, specific as follows:
For Rynaxypyr: green muscardine fungus=0.2:0.8, if Rynaxypyr is A, green muscardine fungus is B, and using Rynaxypyr (A) as standard agent, green muscardine fungus (B) is as reagent agent B.
The toxicity index TIA of Rynaxypyr is 100;
Toxicity index TIB × green muscardine fungus mass percent=100 in mixture × 2/10+3173 × 8/10=2558.4 of the mass percent+green muscardine fungus of toxicity index TIA × Rynaxypyr in mixture of theoretical toxicity index (the TTI)=Rynaxypyr of mixture
As can be seen from the above, Rynaxypyr: the co-toxicity coefficient (CTC)=1860.54 of green muscardine fungus=0.2:0.8 conbined usage.
Calculate Rynaxypyr according to the method described above: the co-toxicity coefficient (CTC) of green muscardine fungus=0.8:0.2 conbined usage and Rynaxypyr: the co-toxicity coefficient (CTC) of green muscardine fungus=0.5:0.5 conbined usage.
Result is as shown in table 3,
Table 3 is Rynaxypyr and green muscardine fungus immixture effect
CTC value criterion according in three of embodiment 14: CTC value is more than or equal to 100 expression mixed agents synergistic effect, and CTC is less than 100 expression mixed agents antagonism;
At Rynaxypyr: under the conbined usage effect of green muscardine fungus=0.2:0.8, co-toxicity coefficient CTC is 1860.54, then Rynaxypyr: the conbined usage of green muscardine fungus=0.2:0.8 act as synergy; At Rynaxypyr: under the conbined usage effect of green muscardine fungus=0.5:0.5, co-toxicity coefficient CTC is 1454.32, then Rynaxypyr: the conbined usage of green muscardine fungus=0.5:0.5 act as synergy.
At Rynaxypyr: under the conbined usage effect of green muscardine fungus=0.8:0.2, co-toxicity coefficient CTC is 33.14, then Rynaxypyr: the conbined usage of green muscardine fungus=0.8:0.2 act as antagonism.
Verify that whether method of the present invention is correct by mortality ratio:
Rynaxypyr: the mortality ratio of the conbined usage of green muscardine fungus=0.2:0.8 as shown in Figure 3, mortality ratio when Rynaxypyr and green muscardine fungus immixture is greater than the mortality ratio of Rynaxypyr or green muscardine fungus independent role, the mortality ratio that such as 0.5mg/L Rynaxypyr acts solely on migratory locusts is 31.11%, the mortality ratio that the green muscardine fungus of 2.50E+07 spore quantity/mL acts solely on migratory locusts is 68.83%, when the ratio mixing with 0.2 and 0.8 of the green muscardine fungus of 0.5mg/L Rynaxypyr and 2.5E+07 spore quantity/mL and 0.1mg/L+2.0E+07 spore quantity/mL mortality ratio for 84.00%, there is synergistic effect.
Rynaxypyr: the mortality ratio of the conbined usage of green muscardine fungus=0.5:0.5 as shown in Figure 4, when the ratio mixing with 0.5 and 0.5 of the green muscardine fungus of 0.5mg/L Rynaxypyr and 2.5E+07 spore quantity/mL and 0.025mg/L+1.25E+06 spore quantity/mL mortality ratio be 48.05% (independent 2.5E+06 spore quantity/mL mortality ratio just 15.58%), there is synergistic effect.
Rynaxypyr: the mortality ratio of the conbined usage of green muscardine fungus=0.8:0.2 as shown in Figure 5, when the ratio mixing with 0.8 and 0.2 of the green muscardine fungus of 0.5mg/L Rynaxypyr and 2.5E+07 spore quantity/mL and 0.4mg/L+0.5E+07 spore quantity/mL mortality ratio for 17.11%, there is antagonism.
In addition, reflect from LC50: LC after green muscardine fungus independent role migratory locusts
50=0.75E+07 spore quantity/mL, Rynaxypyr: the LC of green muscardine fungus during green muscardine fungus=0.2:0.8
50=4.0E+05 spore quantity/mL, Rynaxypyr: the LC of green muscardine fungus during green muscardine fungus=0.5:0.5
50=5.0E+05 spore quantity/mL, the LC of green muscardine fungus when this two kinds of blending ratios
50value is less than LC after green muscardine fungus independent role migratory locusts
50value, also proves to there is synergistic effect; Rynaxypyr: the LC of green muscardine fungus during green muscardine fungus=0.8:0.2
50=2.01E+07 spore quantity/mL is greater than LC after green muscardine fungus independent role migratory locusts
50value, illustrates to there is antagonism.
As can be seen from the above, evaluation method of the present invention is correct.
Claims (4)
1. identify a method for chemical pesticide and green muscardine fungus coupling insecticidal effect, comprise the steps:
1) respectively following A, B and C tri-groups of process: A are carried out to worm, chemical pesticide process separately, B, green muscardine fungus process separately, C chemical pesticide and green muscardine fungus coupling aftertreatment;
Plant raising worm after described chemical pesticide is treated to separately and soaks with chemical pesticide solution;
Described green muscardine fungus is treated to separately sprays worm with muscardine spore suspension;
Described chemical pesticide and green muscardine fungus coupling aftertreatment are first spray worm with muscardine spore suspension, obtain the worm after green muscardine fungus process; Plant raising after again the worm after described green muscardine fungus process being soaked with chemical pesticide solution;
2) detect the mortality ratio that described A, B and C tri-groups respectively organizes the rear worm of process, calculate A group LC
50A, B group LC
50Bwith C group LC
50AB; And at calculating LC
50Band LC
50ABtime, described muscardine spore suspension concentration is converted into the concentration consistent with described chemical pesticide solution concentration unit by transfer equation;
Described transfer equation is y=2E-08x, and wherein, x is muscardine spore suspension concentration, and y is the muscardine spore suspension concentration after conversion; 3) by described LC
50Avalue and described LC
50ABco-toxicity coefficient CTC value by following formulae discovery chemical pesticide and green muscardine fungus coupling group:
Wherein, the actual toxicity index of described mixture
Number percent+green muscardine fungus TI × green muscardine fungus that described mixture theory toxicity index TTI=chemical pesticide TI × chemical pesticide accounts for mixture accounts for the number percent of mixture;
Described chemical pesticide TI is 100, and described green muscardine fungus
Or described green muscardine fungus TI is 100, and described chemical pesticide
The number percent that described chemical pesticide accounts for mixture is chemical pesticide solution concentration/(the muscardine spore suspension concentration after chemical pesticide solution concentration+conversion);
The number percent that described green muscardine fungus accounts for mixture is muscardine spore suspension concentration/(the muscardine spore suspension concentration after chemical pesticide solution concentration+conversion) after conversion;
4) according to following standard determination chemical pesticide and green muscardine fungus coupling insecticidal effect: if described co-toxicity coefficient is more than or equal to 100, then the insecticidal effect of described chemical pesticide and green muscardine fungus coupling is synergy; If described co-toxicity coefficient is less than 100, then the insecticidal effect of described chemical pesticide and green muscardine fungus coupling is antagonism;
Described synergy is that chemical pesticide and green muscardine fungus coupling insecticidal effect are more than or equal to the insecticidal effect that chemical pesticide processes separately or green muscardine fungus processes separately;
Described antagonism is that chemical pesticide and green muscardine fungus coupling insecticidal effect are less than the insecticidal effect that described chemical pesticide processes separately or described green muscardine fungus processes separately.
2. method according to claim 1, is characterized in that:
Described muscardine spore suspension concentration values unit is spore quantity/ml;
Described change after muscardine spore suspension concentration values unit be mg/L;
Described LC
50A, LC
50Bwith described LC
50ABnumerical value unit is mg/L.
3. method according to claim 1 and 2, is characterized in that: described worm is the nymph of a locust;
Described chemical pesticide is Rynaxypyr.
4., according to described method arbitrary in claim 1-3, it is characterized in that:
In described Rynaxypyr and green muscardine fungus coupling processed group, described chemical pesticide solution concentration and described change after the mass ratio of muscardine spore suspension be 1:4,1:1 or 4:1.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106701891A (en) * | 2016-12-01 | 2017-05-24 | 中国农业科学院植物保护研究所 | Detection method for insect control effect of chemical agent and Metarhizium combination |
| CN108398524A (en) * | 2018-01-15 | 2018-08-14 | 宁波高新区鹏博科技有限公司 | A kind of detection device of agricultural insecticide effect |
| CN109730094A (en) * | 2019-03-12 | 2019-05-10 | 中国农业科学院植物保护研究所 | Metarhizium anisopliae IPPMHBHC-7 composite bacteria agent and its application |
| CN109769861A (en) * | 2019-03-13 | 2019-05-21 | 中国农业科学院植物保护研究所 | Metarhizium anisopliae IPPMHBC-009 composite bacteria agent and its application |
| CN109769859A (en) * | 2019-03-12 | 2019-05-21 | 中国农业科学院植物保护研究所 | Metarhizium anisopliae IPPMHBHC-7 composite bacteria agent and its application |
-
2015
- 2015-05-15 CN CN201510247617.8A patent/CN104865350B/en active Active
Non-Patent Citations (4)
| Title |
|---|
| 孙家宝等: "金龟子绿僵菌与农药混用室内杀虫效果研究", 《北方园艺》 * |
| 张英财等: "18种化学农药与绿僵菌相容性研究", 《中国生物防治学报》 * |
| 胡蓉等: "AcNPV·Bt·En复配剂对甜菜夜蛾幼虫的毒力测定", 《中国生物防治》 * |
| 闫瑞坤等: "3种植物提取物复配对松梢螟幼虫的联合毒力作用", 《中国农学通报》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106701891A (en) * | 2016-12-01 | 2017-05-24 | 中国农业科学院植物保护研究所 | Detection method for insect control effect of chemical agent and Metarhizium combination |
| CN108398524A (en) * | 2018-01-15 | 2018-08-14 | 宁波高新区鹏博科技有限公司 | A kind of detection device of agricultural insecticide effect |
| CN109730094A (en) * | 2019-03-12 | 2019-05-10 | 中国农业科学院植物保护研究所 | Metarhizium anisopliae IPPMHBHC-7 composite bacteria agent and its application |
| CN109769859A (en) * | 2019-03-12 | 2019-05-21 | 中国农业科学院植物保护研究所 | Metarhizium anisopliae IPPMHBHC-7 composite bacteria agent and its application |
| CN109769861A (en) * | 2019-03-13 | 2019-05-21 | 中国农业科学院植物保护研究所 | Metarhizium anisopliae IPPMHBC-009 composite bacteria agent and its application |
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| CN104865350B (en) | 2017-03-29 |
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