CN105454258B - A kind of Pesticidal combination and its application containing rhamnolipid and matrine - Google Patents
A kind of Pesticidal combination and its application containing rhamnolipid and matrine Download PDFInfo
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- CN105454258B CN105454258B CN201510911524.0A CN201510911524A CN105454258B CN 105454258 B CN105454258 B CN 105454258B CN 201510911524 A CN201510911524 A CN 201510911524A CN 105454258 B CN105454258 B CN 105454258B
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- rhamnolipid
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Abstract
The present invention discloses a kind of Pesticidal combination containing rhamnolipid and matrine, and the weight of rhamnolipid and matrine ratio is 1 in the composition:80~80:1.Two kinds of compositions of rhamnolipid and matrine are carried out rational compounding and the obvious Pesticidal combination of synergy are made by the present invention, are strengthened quick-acting, are reduced pesticide cost.The present invention also provides a kind of application of Pesticidal combination containing rhamnolipid and matrine in agricultural chemicals, and stronger to the virulence of the insects such as Semiptera aphid, planthopper, diamondback moth, co-toxicity coefficient is higher, has stronger potentiating pesticidal effect.
Description
Technical field
The invention belongs to technical field of pesticide, and in particular to a kind of Pesticidal combination containing rhamnolipid and matrine and its
Using.
Background technology
Rhamnolipid is a kind of Bio-surface active of biological metabolism property as caused by pseudomonad or Burkholderia class
Agent, rhamnolipid mainly have two aspects in pesticides application：Auxiliary agent and biological pesticide, matrine are agricultural chemical of natural plant, insect one
Denier touches the medicine, that is, benumbs nerve center, then makes polypide protein coagulating.The medicine structure novel and unique, activity are high, application
Scope is also progressively widened.Above two agricultural chemicals found during putting into practice use single dose using matrine or rhamnolipid with when
Between passage, its preventive effect has a significant decline process, and rhamnolipid is expensive, adds cost accounting.
The mixed of insecticide is one of strategy the most frequently used in current Chemical Control of Harmful Insects, but because agriculture chemical compounding has increasing
Effect, it is added, three kinds of completely different results of antagonism, the compounding between even two kinds of agricultural chemicals, due to matching different synergy degree
Also it is different, some even antagonisms.Therefore, the synergy that the compounding of agricultural chemicals needs to select by testing sieve is significantly combined
Thing, people there is no to report on the compounding of rhamnolipid and kuh-seng alkali cpd and application at present.
The content of the invention
It is an object of the invention to provide a kind of matrine Pesticidal combination of sum containing rhamnolipid and its application.The present invention
It is achieved through the following technical solutions.
A kind of Pesticidal combination containing rhamnolipid and matrine, the weight of rhamnolipid and matrine in the composition
Than for 1:80~80:1.
Wherein, as preferable technical scheme, a kind of Pesticidal combination containing rhamnolipid and matrine, the composition
The weight ratio of middle rhamnolipid and matrine is 1:20~20:1.
A kind of Pesticidal combination containing rhamnolipid and matrine, the weight of rhamnolipid and matrine in the composition
Than for 1:10~10:1.
The present invention provides another Pesticidal combination containing rhamnolipid and matrine, in the composition rhamnolipid and
The weight ratio of matrine is 1:20；
Further, the weight of the Pesticidal combination containing rhamnolipid and matrine, rhamnolipid and matrine
Than for 1:10.
The present invention also uses a kind of technical scheme：A kind of Pesticidal combination containing rhamnolipid and matrine is in agricultural chemicals
Using wherein the weight of the rhamnolipid and matrine ratio is 1:80~80:1；As preferable scheme, wherein the sandlwood
The weight of glycolipid and matrine ratio is 1:20~20:1；The weight ratio of the further rhamnolipid and matrine is 1:10~
10:1。
In the present invention, the agricultural chemicals can be the preventing and treating agricultural chemicals of the insects such as Semiptera aphid, planthopper, diamondback moth.
The insecticides main component of the present invention is matrine and rhamnolipid, and the matrine structure is novel solely
Special, activity height, with having the insect no interactions resistance of resistance to existing agricultural chemicals, medicament selectivity is high, to Environment Ecological Safe；It is described
Rhamnolipid is preferable to temperature and chemical environment stability, is used as biological pesticide, has face significantly to change agricultural chemicals hydrophily side
Kind, two kinds of compositions are reasonably compounded in the composition, through a series of screening test, the obvious composition of selective advance,
Strengthen quick-acting, reduce pesticide cost, insecticides of the invention are stronger to the virulence of the Hemipteran pests such as aphid, altogether poison
Coefficient is higher, has stronger synergistic effect, is a kind of mixture preparation efficiently, less toxic, economic, and meaning is promoted with extensive
Justice.
Embodiment
Below by embodiment, the invention will be further described：
A kind of Pesticidal combination containing rhamnolipid and matrine, the weight of rhamnolipid and matrine in the composition
Than for 1:80~80:1.
Further, as preferable technical scheme, a kind of Pesticidal combination containing rhamnolipid and matrine, described group
The weight of rhamnolipid and matrine ratio is 1 in compound:20~20:1；
A kind of Pesticidal combination containing rhamnolipid and matrine, the weight of rhamnolipid and matrine in the composition
Than for 1:10~10:1；
The insecticides of the present invention are compounded two kinds of compositions of matrine and rhamnolipid in the composition, through one
The screening test of series, the obvious composition technical scheme of selective advance.
A kind of Pesticidal combination containing rhamnolipid and matrine, the weight of rhamnolipid and matrine in the composition
Than for 1:20；
Another Pesticidal combination containing rhamnolipid and matrine, the weight of rhamnolipid and matrine in the composition
Amount is than being 1:10；
The above-mentioned Pesticidal combination containing rhamnolipid and matrine is applied in agricultural chemicals and not only increases preventive effect, Er Qiejie
The preventing and treating of the about insect such as cost, Semiptera aphid, planthopper, diamondback moth has very big advantage.
Beneficial effects of the present invention are further illustrated below by specific Toxicity Determination and field drug test.
Experiment 1：Toxicity Determination
1. reagent agent
25% rhamnolipid（Daqing Vertex Chemical Co., Ltd.）；98% matrine（The limited public affairs of Xi'an Rayleigh biotechnology
Department）.
2. selected insect source
Experiment worm sources are picked up from wild cabbage, and the situation for being not in contact with any medicament indoors is first raised with wild cabbage.Raising
Temperature is 25 ± 1 DEG C, photoperiod 16/8h（L/D）.
3. single dose assay method
Aphid is to the sensitivity testing of insecticide using leaching leaf dip method：Reagent agent is first diluted with water to mother liquor, then
5 ~ 6 concentration gradients are diluted to, then taking-up absorbs water after dipping 5s in decoction together by the related blade of the test worm chosen
Paper blots decoction, is put into that advance moisturizing is good to be covered with the disposable plastic culture cup of filter paper, and rim of a cup is coverd with black cloth.Every 30 ~
40 first cups, it is repeated 3 times per concentration, 90 ~ 120 aphids is handled per concentration.Control water process.Culture cup is put after connecing worm
It is 26 ± 1 DEG C to enter temperature, photoperiod 16h:8h(L：D incubator).24h inspection results after rhamnolipid processing；Matrine
24h inspection results after processing.
4. data processing
Toxicity regression formula is calculated using POLO Plus softwares.Calculating parameter includes：Slope b values and standard error, card square,
LC50Value.
5. joint toxicity measuring method
The lab screening of composite formula uses the abundant co-toxicity coefficient methods of Sun Yun：
Toxicity index（TI）=（The LC of standard agent50The LC of/reagent agent50）×100
The actual toxicity index of mixture（ATI）=（LC alone medicament A50LC mixed with B/medicament A50）×100
Mixture theoretical toxicity index（TTI）=ATI（A）×a+ATI（B）×b
a：Represent percentage compositions of the medicament A in mixture；
b：Represent percentage compositions of the medicament B in mixture.
Co-toxicity coefficient CTC=（The actual toxicity index ATI/ mixtures theoretical toxicity index TTI of mixture）×100
According to the regulation of the Institute for the Control of Agrochemicals of the Ministry of Agriculture,PRC, the criterion of mixture formula synergy is：Co-toxicity coefficient is more than 120
Synergistic effect；Co-toxicity coefficient 80-120, it is summation action；Co-toxicity coefficient is less than 80, is antagonism.
The selection of optimum proportioning is with the co-toxicity coefficient and LC of mixture50（Or LD50）To weigh, that is, be total to poison is value two indices
The higher and LC of number50（Or LD50）Value is minimum（It is maximum to survey toxicity index）.
Table 1：The toxicity test result of rhamnolipid and matrine mixture
As it can be seen from table 1 in different ratio mixtures, its co-toxicity coefficient is all higher than 120, shows certain synergy
Effect, wherein rhamnolipid：Matrine 1:Synergistic effect is most obvious when 20, co-toxicity coefficient 323.307；Secondly it is rhamnose
Fat：Matrine 1:Co-toxicity coefficient is 186.075 when 10.Two kinds of rhamnolipid under indoor conditions, matrine medicament different ratios
Result of the test shows, stronger synergistic effect is shown to aphid, wherein using the two ratio as 1:20 and 1:Synergy is imitated when 10
Fruit is best.
Experiment 2：Field efficacy is tested
Respectively with 25% rhamnolipid:0.3% matrine WP=1:20,1：10；0.3% matrine；25% rhamnolipid does field
Between prevent the test of pesticide effectiveness that eliminates aphis.Field test in strict accordance with National Standard of the People's Republic of China (GB/T17980.15-
2000) carry out.Experiment totally 5 processing, each handle 4 repetitions, cell random alignment, plot area 30m2.It is using apparatus
16 liters of sprayer sprayings of AGROLEX SPRAYER HD-400 profits agriculture.The insect population per cell is investigated before dispenser, is selected per cell
5 points, every 4 plants of wild cabbages are listed to make marks.Before dispenser and 1 day after dispenser, 3 days, the 7 days work aphids for investigating each cell
Number.According to dead borer population (insect population-residual borer population i.e. before medicine) is investigated after medicine every time compared with insect population before medicine, each cell is calculated
Revision insect recluced rate, then chemicals treatment district Revision insect recluced rate is compared with blank district and calculates correcting controling effect, and
Rectifying efficacies=[(treatment region Revision insect recluced rate-check plot Revision insect recluced rate)/(1- check plots Revision insect recluced rate)] * 100.
Table 2：Field control effectiveness test data comparison table
CK is expressed as adding any insecticide as one of control sample, as can be seen from Table 2, rhamnolipid and hardship in table 2
Join alkali 1:When 20, the preventive effect after 1,3,7 day respectively reaches 92.7%, 95.3% and 94.7%, 1:When 10, its drug effect reaches respectively
To 89.6%, 92.5% and 89.2%；And when carrying out field efficacy experiment to rhamnolipid or single matrine, after 7 days, its preventive effect is only
83.4% and 88.4% are only reached, and the price of rhamnolipid is higher, and it not is a kind of both economical way that single dose, which uses,.Cause
This, from the point of view of two angles of preventive effect and economics, the compounding use of matrine and rhamnolipid, has very relative to single dose use
Big breakthrough, preventive effect is not only increased, and saved cost, the preventing and treating of the insect such as Semiptera aphid, planthopper, diamondback moth has
Very big advantage.
Although the present invention is disclosed as above with preferred embodiment, embodiment is not for limiting the present invention.Do not taking off
From any equivalence changes or micro adjustment in the spirit and scope of the present invention, done, it has no special contribution, therefore same category
In the protection domain of the present invention.
Claims (7)
- A kind of 1. Pesticidal combination containing rhamnolipid and matrine, it is characterised in that：Rhamnolipid and hardship in the composition The weight ratio for joining alkali is 1:60~20:1.
- A kind of 2. Pesticidal combination containing rhamnolipid and matrine as claimed in claim 1, it is characterised in that：The composition The weight ratio of middle rhamnolipid and matrine is 1:20~20:1.
- A kind of 3. Pesticidal combination containing rhamnolipid and matrine as claimed in claim 1, it is characterised in that：The composition The weight ratio of middle rhamnolipid and matrine is 1:10~10:1.
- A kind of 4. Pesticidal combination containing rhamnolipid and matrine as claimed in claim 2, it is characterised in that：The composition The weight ratio of middle rhamnolipid and matrine is 1:20.
- A kind of 5. Pesticidal combination containing rhamnolipid and matrine as claimed in claim 3, it is characterised in that：The composition The weight ratio of middle rhamnolipid and matrine is 1:10.
- 6. application of the Pesticidal combination containing rhamnolipid and matrine in agricultural chemicals described in claim 1-5 any one.
- 7. application of the Pesticidal combination in agricultural chemicals as claimed in claim 6 containing rhamnolipid and matrine, it is characterised in that： The agricultural chemicals can be Semiptera aphid, planthopper, the preventing and treating agricultural chemicals of diamondback moth insect.
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| CN106719730A (en) * | 2016-12-14 | 2017-05-31 | 西安瑞捷生物科技有限公司 | A kind of composition |
| CN108684674A (en) * | 2018-07-03 | 2018-10-23 | 四川国光农化股份有限公司 | A kind of insecticides, preparation and its application |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102484993A (en) * | 2010-12-02 | 2012-06-06 | 华南农业大学 | Synergistic pesticide composition of matrine and tea saponin |
| CN102578116A (en) * | 2012-01-20 | 2012-07-18 | 山西德威生化有限责任公司 | Compounded botanical synergistic fungicide containing sophocarpidine and osthol and preparation method of compounded botanical synergistic fungicide |
| CN103202295A (en) * | 2010-03-30 | 2013-07-17 | 湖州紫金生物科技有限公司 | Application of rhamnolipid as biological pesticide and biological insecticide |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103202295A (en) * | 2010-03-30 | 2013-07-17 | 湖州紫金生物科技有限公司 | Application of rhamnolipid as biological pesticide and biological insecticide |
| CN102484993A (en) * | 2010-12-02 | 2012-06-06 | 华南农业大学 | Synergistic pesticide composition of matrine and tea saponin |
| CN102578116A (en) * | 2012-01-20 | 2012-07-18 | 山西德威生化有限责任公司 | Compounded botanical synergistic fungicide containing sophocarpidine and osthol and preparation method of compounded botanical synergistic fungicide |
Non-Patent Citations (1)
| Title |
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| 1.3 % 苦参碱水剂防治甘蓝蚜虫田间效果试验;侯云龙等;《种子世界》;20140110;第24页摘要 * |
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