CN106719730A - A kind of composition - Google Patents

A kind of composition Download PDF

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Publication number
CN106719730A
CN106719730A CN201611152792.XA CN201611152792A CN106719730A CN 106719730 A CN106719730 A CN 106719730A CN 201611152792 A CN201611152792 A CN 201611152792A CN 106719730 A CN106719730 A CN 106719730A
Authority
CN
China
Prior art keywords
composition
rhamnolipid
nimbin
effect
insect
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201611152792.XA
Other languages
Chinese (zh)
Inventor
陈富林
孙叶芳
薛姝雯
陈新玲
李晶
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
XI'AN REJE BIOLOGICAL TECHNOLOGY Co Ltd
Original Assignee
XI'AN REJE BIOLOGICAL TECHNOLOGY Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by XI'AN REJE BIOLOGICAL TECHNOLOGY Co Ltd filed Critical XI'AN REJE BIOLOGICAL TECHNOLOGY Co Ltd
Priority to CN201611152792.XA priority Critical patent/CN106719730A/en
Publication of CN106719730A publication Critical patent/CN106719730A/en
Pending legal-status Critical Current

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Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/90Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/14Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
    • A01N43/16Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom

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  • Life Sciences & Earth Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Catching Or Destruction (AREA)

Abstract

The invention belongs to technical field of pesticide, more particularly to a kind of composition, including rhamnolipid and nimbin, rhamnolipid and nimbin weight ratio in the composition are 1:1~1:20.Solve simple in the prior art rhamnolipid or nimbin is used alone the resistance that insect has occurred, so that the low technical problem of killing rate, so as to reduce the production cost of insecticide while insecticide efficiency is improved.

Description

A kind of composition
Technical field
The invention belongs to technical field of pesticide, more particularly to a kind of composition and its application.
Background technology
Rhamnolipid is a kind of Bio-surface active of the biological metabolism property produced by pseudomonad or Burkholderia class Agent, it is not only soluble in methyl alcohol, chloroform and ether, and good dissolution characteristics are also shown in alkaline aqueous solution.It has concurrently well Chemistry and biological nature.It is amphipathic with oil, water, water surface tension can be reduced, can be as wetting agent, emulsifying agent and hair Infusion is used, and rhamnolipid biological surface activator can be used in the case where temperature, pH value and salinity are in extreme situation, and nothing Poison, can be with biodegradation.Rhamnolipid mainly has two aspects in pesticides application:Auxiliary agent and biological pesticide.Nimbin is natural plant Thing agricultural chemicals, can make insect produce Antifeedant Effects;Cause that not enough, influence is changed in the nutrition of food by the effect to midgut digestive enzymes The vitality of insect.The nimbin of high dose can directly kill the insects such as bedbug, flea, and low dosage then causes appearance permanent Larva, or deformity pupa, adult etc..The medicine structure novel and unique, activity are high, range of application is also progressively widened.
Existing application of the rhamnolipid in terms of agricultural chemicals in currently available technology, but it is used alone insecticidal effect and has very much Limit, and its manufacturing cost is higher.Nimbin has also had used in connection with terms of desinsection, but it is by after long-time use Insect has been provided with resistance, and insecticidal effect has also been decreased obviously, thus in the urgent need to one kind can effectively insect prevention can reduce again The pharmaceutical composition of pesticide cost solves this technical problem.Synergy, addition, short of money often occurs during agriculture chemical compounding Resist three kinds of different results, therefore need constantly to grope experiment in medicine compound process, find most suitable medicament categories compounding and Most suitable drug ratios compounding, the final composition of medicine for realizing to have gain effect, the realization of this process needs mass data Experiment and pest-resistant screening experiment could be realized.
The content of the invention
The invention belongs to technical field of pesticide, there is provided a kind of insecticidal effect is more preferable, the lower composition of manufacturing cost, and The application of this composition.Solve simple in the prior art rhamnolipid and nimbin is used alone what insect had occurred Resistance problem, so as to effectively improve insecticide efficiency, and reduces agricultural chemicals manufacturing cost.
The technical scheme is that, a kind of composition, including rhamnolipid and nimbin, the rhamnolipid in composition It is 1 with nimbin weight ratio:1~1:20.
Rhamnolipid and nimbin weight ratio preferably 1 in composition:10.
Rhamnolipid and nimbin weight ratio preferably 1 in composition:20.
Above-mentioned composition is applied to agricultural chemicals aspect.
Above-mentioned composition is mainly for insects such as aphid, greenhouse plant hopper, diamondback moth, cabbage caterpillar, red spider, smaller green leaf hoppers Preventing and treating.
Above-mentioned composition is preferably applied to be the preventing and treating to aphid.
A kind of insecticide, comprising above-mentioned composition.
A kind of disinsection method, by above-mentioned composition dilution, 800~1000 times are sprayed on plant leaf surface.
The present invention is tested by the combination to rhamnolipid and nimbin, tested, being contrasted, and forms a kind of combination Thing, is applied to desinsection field, and as insecticide, its manufacturing cost is lower, and being used in combination for the two makes insecticidal effect more independent Significantly improved using insecticide efficiency, and manufacturing cost is lower.And overcome resistance of the existing insect to common agricultural chemicals, desinsection effect Fruit is more preferable, and to person poultry harmless.
Specific embodiment
To enable the above objects, features and advantages of the present invention more obvious understandable, below to specific reality of the invention The mode of applying elaborates.Elaborate many details in order to fully understand the present invention in the following description.But this Invention can be implemented with being much different from other manner described here, and those skilled in the art can be without prejudice to the present invention Similar improvement is done in the case of intension, therefore the present invention is not limited by following public specific embodiment.
A kind of composition of embodiment 1, including rhamnolipid and nimbin, rhamnolipid and nimbin wherein in composition Weight ratio is 1:1.Can ensure that the two combination is not in antagonism in this ratio range, and the two is matched with this Obvious gain effect is served after combination in terms of the insecticidal effect of insect.By experiment also demonstrate that the two with weight ratio be 1: After 1 proportioning is combined, rhamnolipid relatively is used alone for its insecticidal effect or nimbin insecticidal effect is obviously improved, although this is matched somebody with somebody Compare the two be used alone gain effect it is more obvious, can economically consider, because the current manufacturing cost of rhamnolipid compared with Height, so rhamnolipid or nimbin weight ratio are with 1:The composition of 1 proportion relation production, its production cost is still higher. But the proportioning also makes insecticidal effect more lasting, using complete said composition desinsection after, lose within nearly 27 days on plant insect growth. Again because of rhamnolipid and the special property of medicine of nimbin, therefore the composition of the two combination production is endangered people and animals in use It is small, harm will not be brought to the mankind or livestock because the mankind or livestock eat by mistake.
A kind of composition of embodiment 2, including rhamnolipid and nimbin, rhamnolipid and nimbin weight in composition Than being 1:10.This proportioning is the optimal proportion in the present invention, and this ratio can ensure that the two combination is not in that antagonism is made With, and by after experiment, experimental result also demonstrate rhamnolipid and nimbin with weight ratio be 1:10 proportion relation knot After conjunction, on the premise of insecticidal effect is declined to a great extent, the production cost of whole composition is but substantially reduced.The present embodiment Proportioning selection, not only reduces the production cost of composition, and the insecticidal effect of its composition also compares exclusive use, gain Effect is obvious.Therefore this programme is the most suitable scheme of the application, and most suitable balance is have found between insecticidal effect and production cost Point.This programme not only good disinsection effect but also low production cost.
A kind of composition of embodiment 3, including rhamnolipid and nimbin, rhamnolipid and nimbin weight in composition Than being 1:20.This proportioning makes the production cost of composition cheaper, and this antagonism does not occur yet, the combination of the two There is gain effect using being also relatively used alone, the insecticidal effect of comparable proportioning before will be nothing like, such as with 1:10 this It is individual most adaptation than compare the desinsection number of the two differed to the greatest extent 1/5th, although therefore this ratio proportioning manufacturing cost It is low, can insecticidal effect it is unsatisfactory.But the insecticidal effect of its exclusive use of rhamnolipid and nimbin more in the prior art Or slightly improve, and its low production cost, it may have certain economic worth.
Composition in the present invention is applied to agricultural chemicals by embodiment 4, for killing the insect such as aphid, temperature that are grown on plant Room plant hopper, diamondback moth, cabbage caterpillar, red spider and smaller green leaf hopper insect etc., its insecticidal effect is also used alone more in the prior art The insecticidal effect of the insecticide of rhamnolipid and nimbin has clear improvement, and solves long-term use prior art Pesticides, and The resistance problem that insect produces.
Composition in the present invention is applied to agricultural chemicals by embodiment 5, is preferably applied to the preventing and treating to aphid, its preventing and treating effect Preferably, when preventing and treating other insects, also indivedual insects survive fruit, and its Rectifying efficacies nearly reaches during to control of aphids To 98%.
Embodiment 6 is present invention also offers a kind of insecticide, composition of the insecticide comprising the present patent application, its desinsection Effect is good, and low production cost.
A kind of disinsection method of embodiment 7, is sprayed after the composition clear water comprising the application is diluted into 800 times with spraying machine In on plant leaf surface, the method insecticidal effect is preferable, but medicine high cost.
A kind of disinsection method of embodiment 8, plant is sprayed on after the composition clear water of the application is diluted into 900 times with spraying machine On thing blade face, the disinsection method reduces the production cost of insecticide while insecticidal effect is slightly reduced, be the application most Suitable disinsection method.
A kind of disinsection method of embodiment 9, plant is sprayed on after diluting 1000 times comprising composition clear water with spraying machine On blade face, the method low production cost, though the two is used alone more in the prior art through diluting the good disinsection effect after 1000 times, But compared to first two method, its insecticidal effect is substantially not enough.
Experimental data:In mid-July, 2015 in Shaanxi Province Yanliang District pollution-free vegetable production base, choose middle fertility, PH value be 6.5-8.0 between, soil property be blue mud soil vegetable field, choose 667 ㎡, averagely plantation 3000 plants of wild cabbages, it is sweet at the beginning of 10 months Blue growing way is essentially identical, proceeds by insecticide experiment.It is 1 by the weight proportion of rhamnolipid and nimbin:1、1:10、1:20 Composition carry out the dilution of same equimultiple with the medicament that rhamnolipid and exclusive use nimbin is used alone respectively, it is then right Wild cabbage carries out sprinkling experiment.
3 areas of experiment point, each area's area is 100 ㎡, and each area is divided into 4 cells again, and random alignment, each area is carried out The preventive effect test of various concentrations proportioning, same equimultiple is carried out by the composition and the exclusive use of the two of rhamnolipid and nimbin Using clear water as control after dilution, tested.Quantitative plant is randomly selected before spray carries out the record of number of pest.
Aphed population, then carries out dispenser to experimental subjects in preferably several days in weather using knapsack spraying agent, and 3rd day statistics aphid survival number situation after spray.
Drug effect computational methods:Insect population goes down=(worm amount after worm amount-medicine before medicine)/medicine before worm amount × 100%
Rectifying efficacies=(each treatment region Revision insect recluced rate-clear water control Revision insect recluced rate)/(1- clear water control insect population goes down Rate)
It is now discovered that rhamnolipid and nimbin with weight ratio be 1:10 proportion relation is prevented through 900 times of desinsections for using of dilution Efficiencies are optimal, and the experimental data for now providing this group see the table below:
The Pesticidal combination of nimbin and rhamnolipid test result indicate that, nimbin and rhamnolipid are 1 in weight ratio: During 10 proportioning, it is best practice to consider as the insecticidal effect and production cost of insecticide after being diluted through 900 times.Rhamnose Fat and nimbin are used separately as insecticide, although the desinsection preventive effect all with certain insecticidal effect such as nimbin is reachable 76.55%, and the desinsection preventive effect of rhamnolipid all has certain insecticidal effect compared to clear water control treatment up to 42.97%, And nimbin and rhamnolipid are 1:Killing rate has averagely reached 98.35% mostly close to 100% during 10 proportioning.The two Combine the Pesticidal combination desinsection particularly to aphid in terms of desinsection with significant gain effect, nimbin and rhamnolipid Preventive effect has also reached extremely notable compared to the two exclusive use variance analysis.Have between rhamnolipid and the combination of nimbin obvious Gain effect, and 1:Good insecticidal effect can be ensured when 10, the production cost of composition is significantly reduced again.

Claims (8)

1. a kind of composition, it is characterised in that:Comprising rhamnolipid and nimbin, wherein the weight ratio of rhamnolipid and nimbin It is 1:1~1:20.
2. composition according to claim 1, it is characterised in that:Rhamnolipid and nimbin weight in composition is than excellent Elect 1 as:10.
3. composition according to claim 1, it is characterised in that:Rhamnolipid and nimbin weight in composition is than excellent Elect 1 as:20.
4. the composition according to any one of claim 1-3, it is characterised in that:The composition is used for agricultural chemicals.
5. according to any described composition in claim 1-3, it is characterised in that:The composition is used for aphid, greenhouse The preventing and treating of plant hopper, diamondback moth, cabbage caterpillar, red spider and smaller green leaf hopper insect.
6. according to any described composition in claim 1-3, it is characterised in that:The composition is preferably used in aphid Preventing and treating.
7. a kind of insecticide, it is characterised in that:Comprising any described composition in the claims 1-3.
8. a kind of disinsection method, it is characterised in that:Will any described composition dilution 800 in the claims 1-3~ Plant leaf surface is sprayed on after 1000 times.
CN201611152792.XA 2016-12-14 2016-12-14 A kind of composition Pending CN106719730A (en)

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104095765A (en) * 2013-04-02 2014-10-15 赢创工业集团股份有限公司 Mixture composition comprising rhamnolipids
CN104273143A (en) * 2014-10-15 2015-01-14 济南新起点医药科技有限公司 Liuyangmycin and azadirachtin composition and application
CN104351218A (en) * 2014-10-28 2015-02-18 山东中大药业有限公司 Microcapsule suspension containing nimbin and chitosan oligosaccharide as effective components
CN105454258A (en) * 2015-12-11 2016-04-06 江苏沿海地区农业科学研究所 Insecticidal composition containing rhamnolipid and matrine as well as application of insecticidal composition
US20160278373A1 (en) * 2004-06-01 2016-09-29 Agscitech Inc. Biosurfactant compositions and methods for providing plant nutrients

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20160278373A1 (en) * 2004-06-01 2016-09-29 Agscitech Inc. Biosurfactant compositions and methods for providing plant nutrients
CN104095765A (en) * 2013-04-02 2014-10-15 赢创工业集团股份有限公司 Mixture composition comprising rhamnolipids
CN104273143A (en) * 2014-10-15 2015-01-14 济南新起点医药科技有限公司 Liuyangmycin and azadirachtin composition and application
CN104351218A (en) * 2014-10-28 2015-02-18 山东中大药业有限公司 Microcapsule suspension containing nimbin and chitosan oligosaccharide as effective components
CN105454258A (en) * 2015-12-11 2016-04-06 江苏沿海地区农业科学研究所 Insecticidal composition containing rhamnolipid and matrine as well as application of insecticidal composition

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
徐建陶等: "几种植物源农药对蚜虫的生物活性测定", 《上海农业学报》 *

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Application publication date: 20170531