CN105454258A - Insecticidal composition containing rhamnolipid and matrine as well as application of insecticidal composition - Google Patents
Insecticidal composition containing rhamnolipid and matrine as well as application of insecticidal composition Download PDFInfo
- Publication number
- CN105454258A CN105454258A CN201510911524.0A CN201510911524A CN105454258A CN 105454258 A CN105454258 A CN 105454258A CN 201510911524 A CN201510911524 A CN 201510911524A CN 105454258 A CN105454258 A CN 105454258A
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- CN
- China
- Prior art keywords
- matrine
- rhamnolipid
- insecticidal composition
- weight ratio
- pesticidal combination
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Links
- FCBUKWWQSZQDDI-UHFFFAOYSA-N rhamnolipid Chemical compound CCCCCCCC(CC(O)=O)OC(=O)CC(CCCCCCC)OC1OC(C)C(O)C(O)C1OC1C(O)C(O)C(O)C(C)O1 FCBUKWWQSZQDDI-UHFFFAOYSA-N 0.000 title claims abstract description 65
- ZSBXGIUJOOQZMP-UHFFFAOYSA-N Isomatrine Natural products C1CCC2CN3C(=O)CCCC3C3C2N1CCC3 ZSBXGIUJOOQZMP-UHFFFAOYSA-N 0.000 title claims abstract description 62
- 229930014456 matrine Natural products 0.000 title claims abstract description 62
- ZSBXGIUJOOQZMP-JLNYLFASSA-N Matrine Chemical compound C1CC[C@H]2CN3C(=O)CCC[C@@H]3[C@@H]3[C@H]2N1CCC3 ZSBXGIUJOOQZMP-JLNYLFASSA-N 0.000 title claims abstract description 61
- 239000000203 mixture Substances 0.000 title claims abstract description 41
- 230000000749 insecticidal effect Effects 0.000 title abstract 7
- 241001124076 Aphididae Species 0.000 claims abstract description 11
- 241000500437 Plutella xylostella Species 0.000 claims abstract description 5
- 230000000361 pesticidal effect Effects 0.000 claims description 22
- 239000003905 agrochemical Substances 0.000 claims description 15
- 241000176086 Sogatella furcifera Species 0.000 claims description 4
- 230000002195 synergetic effect Effects 0.000 abstract description 8
- 239000000575 pesticide Substances 0.000 abstract description 6
- 241000607479 Yersinia pestis Species 0.000 abstract description 2
- 241001466042 Fulgoromorpha Species 0.000 abstract 1
- 240000007594 Oryza sativa Species 0.000 abstract 1
- 235000007164 Oryza sativa Nutrition 0.000 abstract 1
- 231100000171 higher toxicity Toxicity 0.000 abstract 1
- 235000009566 rice Nutrition 0.000 abstract 1
- 241000238631 Hexapoda Species 0.000 description 14
- 239000003814 drug Substances 0.000 description 12
- 238000000034 method Methods 0.000 description 10
- 231100000419 toxicity Toxicity 0.000 description 10
- 230000001988 toxicity Effects 0.000 description 10
- 239000002131 composite material Substances 0.000 description 7
- 238000012360 testing method Methods 0.000 description 7
- 239000002917 insecticide Substances 0.000 description 6
- 230000003449 preventive effect Effects 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 5
- 239000003795 chemical substances by application Substances 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 235000006008 Brassica napus var napus Nutrition 0.000 description 3
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 description 3
- 241001674939 Caulanthus Species 0.000 description 3
- 230000008485 antagonism Effects 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 238000012216 screening Methods 0.000 description 3
- 230000000853 biopesticidal effect Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000011835 investigation Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 241001453380 Burkholderia Species 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 241000258937 Hemiptera Species 0.000 description 1
- 206010034133 Pathogen resistance Diseases 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 239000012752 auxiliary agent Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 239000003876 biosurfactant Substances 0.000 description 1
- 230000001112 coagulating effect Effects 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000000857 drug effect Effects 0.000 description 1
- 238000003255 drug test Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- -1 matrine compound Chemical class 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- 239000012452 mother liquor Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 231100000820 toxicity test Toxicity 0.000 description 1
- 230000001018 virulence Effects 0.000 description 1
- 230000010148 water-pollination Effects 0.000 description 1
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses insecticidal composition containing rhamnolipid and matrine. The composition comprises the rhamnolipid and the matrine in the weight ratio being (1:80)-(80:1). Two components, namely, the rhamnolipid and the matrine, are compounded reasonably, the insecticidal composition having the obvious synergistic effect is prepared, the fast-acting property is enhanced, and the pesticide cost is reduced. The invention further provides an application of the insecticidal composition containing the rhamnolipid and the matrine to pesticides. The insecticidal composition has higher toxicity to pests such as Hemiptera aphids, rice planthoppers, Plutella xylostella and the like, has the larger co-toxicity coefficient and has the better synergistic insecticidal effect.
Description
Technical field
The invention belongs to technical field of pesticide, be specifically related to a kind of Pesticidal combination and the application thereof that contain rhamnolipid and matrine.
Background technology
Rhamnolipid is the biosurfactant of a kind of biological metabolism character produced by pseudomonad or Burkholderia class, rhamnolipid mainly contains in two at pesticides application: auxiliary agent and biopesticide, matrine is agricultural chemical of natural plant, insect is once touch this medicine, namely benumb nerve center, then make polypide protein coagulating.This medicine novel structure is unique, activity is high, range of application is also progressively widened.Above-mentioned two kinds of agricultural chemicals find that As time goes on single dose uses matrine or rhamnolipid putting into practice in use procedure, and its preventive effect has a significant decline process, and rhamnolipid is expensive, adds cost accounting.
Insecticide mixed is one of strategy the most frequently used in current Chemical Control of Harmful Insects, but because agriculture chemical compounding has synergy, addition, antagonism three kinds of distinct results, even if composite between two kinds of agricultural chemicals, because the different synergy degree of proportioning is also different, have or even antagonism.Therefore, the obvious composition of the composite synergy needing to be selected by testing sieve of agricultural chemicals, be there is no people about the composite of rhamnolipid and matrine compound and application at present and reported.
Summary of the invention
The object of the present invention is to provide a kind of containing rhamnolipid and matrine Pesticidal combination and application.The present invention is achieved by the following technical solutions.
Contain a Pesticidal combination for rhamnolipid and matrine, in described composition, the weight ratio of rhamnolipid and matrine is 1:80 ~ 80:1.
Wherein, as preferred technical scheme, a kind of Pesticidal combination containing rhamnolipid and matrine, in described composition, the weight ratio of rhamnolipid and matrine is 1:20 ~ 20:1.
Contain a Pesticidal combination for rhamnolipid and matrine, in described composition, the weight ratio of rhamnolipid and matrine is 1:10 ~ 10:1.
The invention provides the Pesticidal combination that another contains rhamnolipid and matrine, in described composition, the weight ratio of rhamnolipid and matrine is 1:20;
Further, the described Pesticidal combination containing rhamnolipid and matrine, the weight ratio of rhamnolipid and matrine is 1:10.
The present invention also adopts a kind of technical scheme: a kind of application of Pesticidal combination in agricultural chemicals containing rhamnolipid and matrine, and the weight ratio of wherein said rhamnolipid and matrine is 1:80 ~ 80:1; As preferred scheme, the weight ratio of wherein said rhamnolipid and matrine is 1:20 ~ 20:1; The weight ratio of further described rhamnolipid and matrine is 1:10 ~ 10:1.
In the present invention, described agricultural chemicals can be the control agricultural chemicals of the insects such as Semiptera aphid, rice fulgorid, diamond-back moth.
Insecticides main component of the present invention is matrine and rhamnolipid, and described matrine novel structure is unique, active high, with to the insect of existing agricultural chemicals tool resistance without cross resistance, medicament selectivity is high, to Environment Ecological Safe; Described rhamnolipid to temperature and chemical environment stability better, use as biopesticide, face is had significantly to improve to agricultural chemicals hydrophily side, two kinds of compositions carry out reasonably composite in the composition, through a series of screening test, the obvious composition of selective advance, strengthen quick-acting, reduce pesticide cost, insecticides of the present invention is comparatively strong to the virulence of the Hemipteran pest such as aphid, and co-toxicity coefficient is higher, has stronger synergistic effect, be that a class is efficient, low toxicity, economic mixture preparation, there is dissemination widely.
Embodiment
Below by embodiment, the invention will be further described:
Contain a Pesticidal combination for rhamnolipid and matrine, in described composition, the weight ratio of rhamnolipid and matrine is 1:80 ~ 80:1.
Further, as preferred technical scheme, a kind of Pesticidal combination containing rhamnolipid and matrine, in described composition, the weight ratio of rhamnolipid and matrine is 1:20 ~ 20:1;
Contain a Pesticidal combination for rhamnolipid and matrine, in described composition, the weight ratio of rhamnolipid and matrine is 1:10 ~ 10:1;
Matrine and rhamnolipid two kinds of compositions carry out composite by insecticides of the present invention in the composition, through a series of screening test, and the obvious composition technical scheme of selective advance.
Contain a Pesticidal combination for rhamnolipid and matrine, in described composition, the weight ratio of rhamnolipid and matrine is 1:20;
Another contains the Pesticidal combination of rhamnolipid and matrine, and in described composition, the weight ratio of rhamnolipid and matrine is 1:10;
Be applied in agricultural chemicals by the above-mentioned Pesticidal combination containing rhamnolipid and matrine and not only increase preventive effect, and saved cost, the control of the insects such as Semiptera aphid, rice fulgorid, diamond-back moth has very large advantage.
Beneficial effect of the present invention is further illustrated below by concrete Toxicity Determination and field drug test.
Experiment 1: Toxicity Determination
1. reagent agent
25% rhamnolipid (Daqing Vertex Chemical Co., Ltd.); 98% matrine (Rayleigh, Xi'an bio tech ltd).
2. for examination worm source
Experiment worm source is picked up from wild cabbage, and the situation not contacting any medicament in indoor is first raised with wild cabbage.Raising temperature is 25 ± 1 DEG C, and the photoperiod is 16/8h(L/D).
3. single dose assay method
Aphid adopts leaching leaf dip method to the sensitivity testing of insecticide: first reagent agent is diluted with water to mother liquor, be diluted to 5 ~ 6 concentration gradients again, after the more related blade of examination worm chosen being flooded 5s together in liquid, taking-up blotting paper blots liquid, the disposable plastic being covered with filter paper putting into moisturizing in advance good cultivates cup, and rim of a cup black cloth is coverd with.Every 30 ~ 40 first cups, every concentration repeats 3 times, every concentration process 90 ~ 120 aphids.Contrast water treatment.Cultivation cup being put into temperature after connecing worm is 26 ± 1 DEG C, and the photoperiod is the incubator of 16h:8h (L:D).24h check result after rhamnolipid process; 24h check result after matrine process.
4. data processing
POLOPlus software is adopted to calculate toxicity regression formula.Calculating parameter comprises: slope b value and standard error, card square, LC
50value.
5. joint toxicity measuring method
The lab screening of composite formula adopts the abundant co-toxicity coefficient method of Sun Yun:
Toxicity index (TI)=(LC of standard agent
50the LC of/reagent agent
50) × 100
The actual toxicity index of mixture (ATI)=(LC that medicament A is alone
50the LC that/medicament A with B is mixed
50) × 100
Theoretical toxicity index (the TTI)=ATI(A of mixture) × a+ATI(B) × b
A: represent the percentage composition of medicament A in mixture;
B: represent the percentage composition of medicament B in mixture.
The theoretical toxicity index TTI of the actual toxicity index ATI/ mixture of co-toxicity coefficient CTC=(mixture) × 100
According to the regulation of the Institute for the Control of Agrochemicals of the Ministry of Agriculture,PRC, the criterion of mixture formula synergy is: co-toxicity coefficient is greater than 120 for synergistic effect; Co-toxicity coefficient 80-120 is summation action; Co-toxicity coefficient is less than 80, is antagonism.
The selection of optimum proportioning is with the co-toxicity coefficient of mixture and LC
50(or LD
50) value two indices weighs, i.e. higher the and LC of co-toxicity coefficient
50(or LD
50) value minimum (namely surveying toxicity index maximum).
Table 1: the toxicity test result of rhamnolipid and matrine mixture
As can be seen from Table 1, in different ratio mixtures, its co-toxicity coefficient is all greater than 120, shows certain synergistic effect, wherein rhamnolipid: during matrine 1:20, synergistic effect is the most obvious, and co-toxicity coefficient is 323.307; Secondly be rhamnolipid: during matrine 1:10, co-toxicity coefficient is 186.075.Under indoor conditions, the result of the test of rhamnolipid, matrine two kinds of medicament different ratio shows, all shows stronger synergistic effect to aphid, wherein with the two ratio for synergistic effect during 1:20 and 1:10 is best.
Experiment 2: field efficacy is tested
Use 25% rhamnolipid respectively: 0.3% matrine WP=1:20,1:10; 0.3% matrine; 25% rhamnolipid does the test of pesticide effectiveness of field control aphid.Field trial is carried out in strict accordance with National Standard of the People's Republic of China (GB/T17980.15-2000).Experiment totally 5 process, each process repeats for 4 times, community randomized arrangement, plot area 30m
2.Use apparatus for AGROLEXSPRAYERHD-400 profit agriculture 16 liters of sprayer sprayings.The insect population radix of the every community of investigation before dispenser, 5 points are selected in every community, and often 4 strain wild cabbages, list and make marks.The aphid number alive of each community within 1 day, 3 days, 7 days, is investigated before dispenser and after dispenser.According to the dead borer population of each investigation (i.e. insect population radix-residual borer population before medicine) after medicine compared with insect population radix before medicine, calculate each community Revision insect recluced rate, then chemicals treatment district Revision insect recluced rate is calculated correcting controling effect compared with blank district, and Rectifying efficacies=[(treatment region Revision insect recluced rate-check plot Revision insect recluced rate)/(1-check plot Revision insect recluced rate)] * 100.
Table 2: field control effectiveness test Data Comparison table
In table 2, CK is expressed as and adds any insecticide one of sample in contrast, and as can be seen from Table 2, rhamnolipid and matrine are when 1:20,1, the preventive effect after 3,7 days reaches 92.7%, 95.3% and 94.7% respectively, when 1:10, its drug effect reaches 89.6%, 92.5% and 89.2% respectively; And when carrying out field efficacy experiment to rhamnolipid or single matrine, after 7 days, its preventive effect only reaches 83.4% and 88.4%, and the price of rhamnolipid is higher, it is not a kind of both economical way that single dose uses.Therefore, from preventive effect and economics two angles, the composite use of matrine and rhamnolipid, use relative to single dose and have very large breakthrough, not only increase preventive effect, and saved cost, the control of the insects such as Semiptera aphid, rice fulgorid, diamond-back moth has very large advantage.
Although the present invention with preferred embodiment openly as above, embodiment is not for limiting the present invention.Without departing from the spirit and scope of the invention, any equivalence change done or trace adjustment, it there is no special contribution, therefore belongs to the protection domain of the present invention equally.
Claims (7)
1. contain a Pesticidal combination for rhamnolipid and matrine, it is characterized in that: in described composition, the weight ratio of rhamnolipid and matrine is 1:80 ~ 80:1.
2. a kind of Pesticidal combination containing rhamnolipid and matrine as claimed in claim 1, is characterized in that: in described composition, the weight ratio of rhamnolipid and matrine is 1:20 ~ 20:1.
3. a kind of Pesticidal combination containing rhamnolipid and matrine as claimed in claim 1, is characterized in that: in described composition, the weight ratio of rhamnolipid and matrine is 1:10 ~ 10:1.
4. a kind of Pesticidal combination containing rhamnolipid and matrine as claimed in claim 2, is characterized in that: in described composition, the weight ratio of rhamnolipid and matrine is 1:20.
5. a kind of Pesticidal combination containing rhamnolipid and matrine as claimed in claim 3, is characterized in that: in described composition, the weight ratio of rhamnolipid and matrine is 1:10.
6. described in claim 1-5 any one, contain the application of Pesticidal combination in agricultural chemicals of rhamnolipid and matrine.
7. contain the application of Pesticidal combination in agricultural chemicals of rhamnolipid and matrine as claimed in claim 6, it is characterized in that: described agricultural chemicals can be the control agricultural chemicals of Semiptera aphid, rice fulgorid, diamond-back moth insect.
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|---|---|---|---|
| CN201510911524.0A CN105454258B (en) | 2015-12-11 | 2015-12-11 | A kind of Pesticidal combination and its application containing rhamnolipid and matrine |
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| CN105454258A true CN105454258A (en) | 2016-04-06 |
| CN105454258B CN105454258B (en) | 2017-11-24 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106719730A (en) * | 2016-12-14 | 2017-05-31 | 西安瑞捷生物科技有限公司 | A kind of composition |
| CN108684674A (en) * | 2018-07-03 | 2018-10-23 | 四川国光农化股份有限公司 | A kind of insecticides, preparation and its application |
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| CN102484993A (en) * | 2010-12-02 | 2012-06-06 | 华南农业大学 | Synergistic pesticide composition of matrine and tea saponin |
| CN102578116A (en) * | 2012-01-20 | 2012-07-18 | 山西德威生化有限责任公司 | Compounded botanical synergistic fungicide containing sophocarpidine and osthol and preparation method of compounded botanical synergistic fungicide |
| CN103202295A (en) * | 2010-03-30 | 2013-07-17 | 湖州紫金生物科技有限公司 | Application of rhamnolipid as biological pesticide and biological insecticide |
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2015
- 2015-12-11 CN CN201510911524.0A patent/CN105454258B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103202295A (en) * | 2010-03-30 | 2013-07-17 | 湖州紫金生物科技有限公司 | Application of rhamnolipid as biological pesticide and biological insecticide |
| CN102484993A (en) * | 2010-12-02 | 2012-06-06 | 华南农业大学 | Synergistic pesticide composition of matrine and tea saponin |
| CN102578116A (en) * | 2012-01-20 | 2012-07-18 | 山西德威生化有限责任公司 | Compounded botanical synergistic fungicide containing sophocarpidine and osthol and preparation method of compounded botanical synergistic fungicide |
Non-Patent Citations (1)
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106719730A (en) * | 2016-12-14 | 2017-05-31 | 西安瑞捷生物科技有限公司 | A kind of composition |
| CN108684674A (en) * | 2018-07-03 | 2018-10-23 | 四川国光农化股份有限公司 | A kind of insecticides, preparation and its application |
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| Publication number | Publication date |
|---|---|
| CN105454258B (en) | 2017-11-24 |
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