CN104855772A - Maca enzyme and preparation method thereof - Google Patents

Abstract

The invention provides Maca enzyme and a preparation method thereof. The Maca enzyme is prepared from the following components in parts by weight: 65-75 parts of garden stuff, 18-22 parts of fungus, 8-12 parts of Maca, 15-20 parts of sugar and 0.2-0.8 parts of probiotics, and the components are subjected to fermentation to prepare the Maca enzyme. The preparation method comprises the steps of preparing Maca enzymatic hydrolysate, preparing enzyme primary liquid, preparing Maca enzyme and the like. Effect of garden stuff, fungus and Maca composition is further played through enzyme technology, effective components in Maca can be effectively dissolved through Maca enzymolysis method, and the use ratio is increased. Plant enzyme is extracted from pure natural plant through multiple flora mixed fermentation technology. The product contains proteins, amino acids, vitamins, enzymes and the like required by a human body, and various health-care efficacy of enzyme is improved. According to the preparation method, raw materials are subjected to pulping processing, so that the fermentation period is shortened. The invention provides the process method which is simple in operation process and suitable for large-size production of enzyme products.

Description

A kind of agate coffee ferment and preparation method thereof

Technical field

The invention belongs to biofermentation preparing technical field, especially belong to a kind of method utilizing ferment fermentation technique to prepare ferment, be specifically related to a kind of agate coffee ferment and preparation method thereof.

Background technology

Agate coffee be growth Andean a kind of crucifer of Peru's height above sea level 4000 ~ 4500 meters, have long history at mountain area, Andean agate coffee block root as the plant of dietotherapeutic, be conventionally used to and improve fecundity, increase energy.Modern large quantity research shows that agate coffee has and improves the multiple efficacies such as fecundity, antifatigue, improvement function, anti-oxidant, Suppress hyperplasia of prostate, alleviation climacteric metancholia, develop immunitypty.Agate coffee is a kind of food resource having better nutritivity and be worth; protein content in main edible part root is higher; amino acid composition rationally; the trace elements such as essential amino acids content, unrighted acid ratio, potassium, calcium, magnesium, zinc are all higher; and containing a large amount of branched-chain amino acid and a certain amount of taurine, be therefore subject to the extensive concern of countries in the world.Researcher thinks that the secondary metabolites such as alkaloid, glucosinolate, sterol, polyphenol that the plurality of health care functions of agate coffee contains with it has substantial connection, and antioxidation activity is also produce the basis of these effects, therefore the exploitation of research to the announcement of agate coffee other biological functional mechanism, functional product of agate coffee anti-oxidation function and Active regenerator is significant.

Ferment be a kind of be made up of amino acid there is special bioactive material, it is present in the animal and plant body of all work, is to maintain body normal function, digest food, and the one of the vital movements such as repair tissue must material, almost participates in all biological activities.Ferment is divided into acidifying reduction enzyme, transferase, hydrolyzable ferment, departs from the six large classes such as ferment, different in nature ferment and NOS.There is oxidation, decomposition, metabolism, heat energy effect, the effect of purifying the blood, antibacterial defence effect.

Summary of the invention

The invention provides a kind of agate coffee ferment and preparation method thereof.The problem to be solved in the present invention is to provide a kind of agate coffee ferment, the invention provides the function factor of agate coffee and ferment merged by ferment technology prepare nutritious, there is the preparation method strengthening health, improve the agate coffee ferment of the effects such as body immunity.

The present invention is achieved through the following technical solutions:

Agate coffee ferment, is made up of the composition of following parts by weight: fruits and vegetables 65-75 part, mushroom 18-22 part, agate coffee 8-12 part, sugared 15-20 part.

Further described agate coffee ferment, is grouped into by the one-tenth of following parts by weight: fruits and vegetables 70 parts, mushroom 20 parts, agate coffee 20 parts, sugar 8 parts.

Above-mentioned agate coffee ferment adds the fermentation of probio 0.2-0.8 weight portion by the composition of described parts by weight and makes.

Wherein:

Probio comprises one or more mixtures in yeast, acetobacter, lactic acid bacteria.Each probio has commercially available.

Fruits and vegetables comprise one or more the mixture in pawpaw, orange, apple, peach, sweet orange, grape, shaddock, pear, lemon, watermelon, "Hami" melon, orange, pineapple, banana, sugarcane, strawberry, Kiwi berry, dragon fruit, loquat, plum, mango, pomegranate, cabbage, romaine lettuce, asparagus lettuce, Chinese cabbage, cucumber, tomato, ternip, wax gourd, carrot, broccoli, celery, balsam pear, pumpkin.

Mushroom comprises one or more the mixture in flat mushroom, mushroom, auricularia auriculajudae, asparagus, coprinus comatus, pleurotus eryngii, white fungus, matsutake and bacterium powder thereof.

The preparation method of agate coffee ferment: comprise the following steps:

Take each constitutive material of above-mentioned parts by weight;

1) agate coffee enzymolysis liquid is prepared

1. pretreatment: cleaned up by agate coffee, pulled an oar by beater, makes the even particle size of agate coffee;

2. enzymolysis: the complex enzyme formulation getting agate coffee quality 0.8%-1.5%, the pure water adding agate coffee quality 1 times amount in complex enzyme formulation stirs, complex enzyme formulation is merged, complex enzyme formulation is made up of amylase, cellulase, hemicellulase and protease, the weight ratio of amylase, cellulase, hemicellulase and protease is 2:3:4:2, again the complex enzyme formulation after fusion is added in graininess agate coffee, make agate coffee enzymolysis; Hydrolysis temperature controls at 40 DEG C-60 DEG C, and pH controls at 4.5-6, enzymolysis time 1-2.5 hour, obtains agate coffee enzymolysis liquid;

2) ferment just liquid is prepared

Choose fruits and vegetables that fresh nothing rots, mushroom, medicinal and edible plant put into cleaning machine and clean up rear ozonization process, put into beater making beating and load sterilization after fermentation cylinder, add brown sugar, probio, Room-temperature seal fermentation 6-12 month, obtain ferment just liquid;

3) agate coffee ferment is prepared

By above-mentioned steps 1) the agate coffee enzymolysis liquid prepared adds step 2) ferment obtained prepared is just in liquid Normal juice, continue fermentation 6 months, obtain agate coffee ferment.

Compared with prior art, the present invention has the following advantages:

1, the present invention plays effect of fruits and vegetables, mushroom, maca composition further by ferment technology, and in the fermented product of composition, each material effect is fully used, and mutually promote, Synergistic, its compatibility meets traditional Chinese medicine monarch, minister, helps, makes principle, and health-care efficacy is excellent.

2, adopt the method to agate coffee enzymolysis, active ingredient in agate coffee effectively can be dissolved, increase its utilization rate.

3, the present invention adopts many floras mixed culture fermentation technology to extract plant enzyme from pure natural plant, and the protein, amino acid, vitamin, enzyme material etc. of product not only containing needed by human, improve the various health-care efficacies of ferment.

4. in preparation method of the present invention, raw material is carried out making beating process, shorten fermentation period, and it is simple to provide an operating procedure, is applicable to the process of large-scale production ferment product.

Accompanying drawing explanation

Fig. 1 is ferment preparation technology flow chart of the present invention.

Detailed description of the invention

Below by embodiment, the present invention is specifically described; embodiment is only for being further detailed the present invention; can not be interpreted as limiting the scope of the invention, some nonessential improvement that those skilled in the art makes according to the content of the invention described above and adjustment also belong to the scope of protection of the invention.

Composition graphs 1.

Ferment of the present invention is made up of following parts by weight raw material: fruits and vegetables 65-75 part, mushroom 18-22 part, agate coffee 15-20 part, sugared 8-10 part, probio 0.2-0.8 part.

Wherein fruits and vegetables comprise: the mixtures such as pawpaw, orange, apple, peach, sweet orange, grape, shaddock, pear, lemon, watermelon, "Hami" melon, orange, pineapple, banana, sugarcane, strawberry, Kiwi berry, dragon fruit, loquat, plum, mango, pomegranate, cabbage, romaine lettuce, asparagus lettuce, Chinese cabbage, cucumber, tomato, ternip, wax gourd, carrot, broccoli, celery, balsam pear, pumpkin;

Mushroom comprises: the mixtures such as flat mushroom, mushroom, auricularia auriculajudae, asparagus, coprinus comatus, pleurotus eryngii, white fungus, matsutake and bacterium powder thereof;

Probio comprises one or more mixtures in yeast, acetobacter, lactic acid bacteria; .

Above-mentioned raw materials is utilized to prepare the method for agate coffee ferment

1) agate coffee enzymolysis liquid is prepared

1. pretreatment: take 20 parts of agate coffees, clean up, pulled an oar by beater, make the even particle size of agate coffee, be beneficial to enzymolysis and extraction;

2. enzymolysis: the complex enzyme formulation getting agate coffee quality 0.8%-1.5%, the pure water adding agate coffee quality 1 times amount in complex enzyme formulation stirs, complex enzyme formulation is merged, complex enzyme formulation is made up of amylase, cellulase, hemicellulase and protease, the mass ratio of amylase, cellulase, hemicellulase and protease is 2:3:4:2, again the complex enzyme formulation after fusion is added in graininess agate coffee, make agate coffee enzymolysis; Hydrolysis temperature controls at 40 DEG C-60 DEG C, and pH controls at 4.5-6, enzymolysis time 1-2.5 hour, obtains agate coffee enzymolysis liquid;

2) ferment just liquid is prepared

Choose fruits and vegetables 65 parts, the mushroom 12 parts that fresh nothing rots to put into cleaning machine and clean up rear ozonization process, put into beater making beating and load the 250L jar fermenter after sterilizing, add 8 parts, brown sugar, probio 0.6 part, add water 15 parts, Room-temperature seal fermentation 6-12 month, obtains ferment just liquid;

3) agate coffee ferment is prepared

By above-mentioned steps 1) in agate coffee enzymolysis liquid add above-mentioned steps 2) in ferment Normal juice, continue fermentation 6-12 month, obtain agate coffee ferment.

Experimental example

By detecting agate coffee ferment of the present invention, obtain following data:

Title	Unit	Content
			SOD enzyme	U/mL	109
POD enzyme	U/mL	87
			Protease	U/mL	206
Cellulase	U/mL	5
			Amylase	U/mL	237
Alkaloid	％	Trace
			Sterol	mg/kg	Trace
Polyphenol	mg/kg	Trace
			Thick protein	％	0.81
Total amino acid content	％	0.49
			Soluble protein	mg/mL	1.05
Acidity	—	3.51
			Thick many candies	mg/100mL	78
Organic acid (total amount)	mg/100ml	112.59

As can be seen from the above table, agate coffee ferment of the present invention is rich in the active principle compositions such as multiple enzyme, organic acid, amino acid and protein, the composition such as alkaloid, sterol, polyphenol simultaneously containing trace, its rich in nutritive value, has effects such as strengthening health, raising fecundity, enhancing body immunity.

The machined method of agate coffee ferment that the inventive method is obtained, and make agate coffee ferment liquid, paste, granule and tablet.

Claims (7)

1. an agate coffee ferment, is characterized in that being made up of the composition of following parts by weight: fruits and vegetables 65-75 part, mushroom 18-22 part, agate coffee 8-12 part, sugared 15-20 part.

2. agate coffee ferment according to claim 1, is characterized in that being grouped into by the one-tenth of following parts by weight: fruits and vegetables 70 parts, mushroom 20 parts, agate coffee 20 parts, sugar 8 parts.

3. agate coffee ferment according to claim 1 or 2, is characterized in that: drink adds the fermentation of probio 0.2-0.8 weight portion by the composition of described parts by weight and makes.

4. agate coffee ferment according to claim 3, is characterized in that: described probio comprises one or more mixtures in yeast, acetobacter, lactic acid bacteria.

5. agate coffee ferment according to claim 4, is characterized in that: described fruits and vegetables comprise: one or more the mixture in pawpaw, orange, apple, peach, sweet orange, grape, shaddock, pear, lemon, watermelon, "Hami" melon, orange, pineapple, banana, sugarcane, strawberry, Kiwi berry, dragon fruit, loquat, plum, mango, pomegranate, cabbage, romaine lettuce, asparagus lettuce, Chinese cabbage, cucumber, tomato, ternip, wax gourd, carrot, broccoli, celery, balsam pear, pumpkin.

6. agate coffee ferment according to claim 4, is characterized in that: described mushroom comprises one or more the mixture in flat mushroom, mushroom, auricularia auriculajudae, asparagus, coprinus comatus, pleurotus eryngii, white fungus, matsutake and bacterium powder thereof.

7. the preparation method of an agate coffee ferment: it is characterized in that comprising the following steps:

Take each constitutive material of parts by weight described in any one of claim 1 to 6;

1) agate coffee enzymolysis liquid is prepared

1. pretreatment: cleaned up by agate coffee, pulled an oar by beater, makes the even particle size of agate coffee;

2. enzymolysis: the complex enzyme formulation getting agate coffee quality 0.8%-1.5%, the pure water adding agate coffee quality 1 times amount in complex enzyme formulation stirs, complex enzyme formulation is merged, complex enzyme formulation is made up of amylase, cellulase, hemicellulase and protease, the weight ratio of amylase, cellulase, hemicellulase and protease is 2:3:4:2, again the complex enzyme formulation after fusion is added in graininess agate coffee, make agate coffee enzymolysis; Hydrolysis temperature controls at 40 DEG C-60 DEG C, and pH controls at 4.5-6, enzymolysis time 1-2.5 hour, obtains agate coffee enzymolysis liquid;

2) ferment just liquid is prepared

Choose fruits and vegetables that fresh nothing rots, mushroom, medicinal and edible plant put into cleaning machine and clean up rear ozonization process, put into beater making beating and load sterilization after fermentation cylinder, add brown sugar, probio, Room-temperature seal fermentation 6-12 month, obtain ferment just liquid;

3) agate coffee ferment is prepared

By above-mentioned steps 1) the agate coffee enzymolysis liquid prepared adds step 2) ferment obtained prepared is just in liquid Normal juice, continue fermentation 6 months, obtain agate coffee ferment.