CN104855269B - Paddy rice is more than a year for breeding method - Google Patents

Paddy rice is more than a year for breeding method Download PDF

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CN104855269B
CN104855269B CN201510155780.1A CN201510155780A CN104855269B CN 104855269 B CN104855269 B CN 104855269B CN 201510155780 A CN201510155780 A CN 201510155780A CN 104855269 B CN104855269 B CN 104855269B
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rice
seed
breeding
good
paddy rice
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李金军
李军
高荣村
陆金根
李白
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ZHEJIANG JIAXING AGRICULTURAL SCIENCE RESEARCH INSTITUTE
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Abstract

The invention discloses a kind of paddy rice is more than a year for breeding method, the method is comprised the following steps:Water intaking rice, is carried out after Dormancy breaking, vernalization live;Treat that paddy rice enters blooming stage, clever emasculation, pollination operation are cut to female parent;To grouting parameter, seed-grain ripe earliest or brown rice kind are chosen as parent of future generation;According to step (1)~(3) repetition many generations.The present invention adopts direct sowing culture, cuts clever emasculation, brown rice vernalization sowing, salpeter solution seed soaking quickly breaks seed dormancy, accelerates the breeding cultivation mode of seed sprouting, shorten the time of infertility of paddy rice, and then shorten breeding process, paddy rice is realized more than a year for breeding, breeding cycle is long in solving the problems, such as prior art.

Description

Paddy rice is more than a year for breeding method
Technical field
The present invention relates to breeding technical field, more particularly to a kind of breeding method in paddy rice generation more than a year.
Background technology
Paddy rice is one of world's staple food crop, and its yield and quality has a deep effect on the supply of ternational grain and people People's growth in the living standard.Paddy rice is the short day property crop of annual happiness temperature, is adapted in the higher low latitudes of temperature or temperature The higher seasonal growth breeding of degree.Low temperature makes growth retardation, higher temperature make quickening of growing, in addition to temperature, Growth period duration of rice length is mainly still restricted by illumination length, when paddy rice possesses certain nutrient growth amount, if place Within the photoperiod compared with short-day, development is accelerated heading and is shifted to an earlier date, otherwise if being in the sunshine longer photoperiod, development is slowly taken out Fringe postpones, or even can not ear.Most southern several counties and cities of Hainan Province are located in 18 degree or so of north latitude, in 1 year except 4~June it Outward, remaining season sunshine is all shorter, and especially winter temperature is also suitable for paddy growth, likes the spy of warm, short day property according to paddy rice Point, can meet the weather conditions that paddy rice normal growth quickly can be bred again.
Cultivate new rice varieties and be one and the beneficial gene from different parents is aggregating, then by very long chain Lock is exchanged and separates stable process.By existing paddy rice cross breeding breeding technique, develop a new breed, from hybridization combo to property Shape is stably entered to survey and produces identification garden, generally at least needs the promotion of 7~8 generations, and larger for some Traits changes Or affiliation filial generation farther out, then need more promotions from generation to generation become with stable, so very long cultivating process, The maximum middle late rice of China's rice area is occupied especially, due to stronger photonasty, completing one in locality within 1 year Season, though in a numerous season in winter where going strange land temperature higher again, can be only done for two seasons every year, therefore, cultivate one it is stable new Strain 4~5 years at least, at most 6~7 years even for more time.The so very long time constrains the cultivation speed of new varieties significantly Degree, traditional reproduction technique need to improve.
At present, there is a small amount of report for the research of raising technology more than a year with regard to crop, it is specific as follows:
The invention patent mandate document of Application No. 99100489.2 discloses a kind of plant rapid breeding in generation more than a year In vitro rataria is directly induced seedling and is combined with developmental process is shortened by method, the method, saves what the development of seed later stage was consumed Time simultaneously hides the accumulation of the mortifier in respective process, strengthens the sensitiveness for receiving to process;KT process is adopted before seedling replanting, is moved Make only nitrogen source with nitrate nitrogen after cultivation, and give endurable high temperature dry morning and photo-irradiation treatment, control plant nutrient bulk-growth, plus Fast developmental process;Using molecular labeling, in seedling, which is screened, and makes Rapid development technology bring out the best in each other with Marker-assisted selection.
The application for a patent for invention document of Application No. 201310075688.5 discloses a kind of local Dai Yu more than a year of soybean The method of kind, specifically includes following steps:1) beanpod, dried process 2~6 days are plucked within 18~26 days after soybean blossoming;2) take step 1) beanpod after dried process, takes out the rataria in beanpod, and being placed directly on culture medium sprouts rataria;The culture medium is MSB+ glutamine 400-500mg/L+ sucrose 30~50g/L+ agar 6.5g/L, the solid medium of pH=5.85;3) rataria It is transplanted to after sprouting in vermiculite, by pouring Hoagland nutrient solutions so as to seedling and grow;4) to soybean blossoming, Repeat step 1~3.The method is by Immature Embryo Culture of Soybean culture on suitable culture medium so as to which direct sprouting and rooting passes through transplanting Seedling, saves the time consumed by seed maturity, shortens the growth cycle of soybean plant strain, accelerates the process of breeding, improves The efficiency of breeding.
Said method is processed to rataria to shorten the seedling time, and then shortens the crop time of infertility, but above-mentioned side Method does not consider actual production, need to carry out in laboratory, it is impossible to be applied to land for growing field crops, more cannot spread.
The content of the invention
The invention provides a kind of paddy rice is more than a year for breeding method, the method solves paddy rice from the angle of arable farming The long problem of breeding cycle, shortens the breeding time of paddy rice, enables paddy rice to complete within 1 year 3~5 seed selection cycles, accelerates Breeding process, the method are beneficial to large-area popularization.
Paddy rice is comprised the following steps more than a year for breeding method:
(1) water intaking rice, is carried out after Dormancy breaking, vernalization live;
(2) treat that paddy rice enters blooming stage, clever emasculation, pollination operation are cut to female parent;
(3) to grouting parameter, seed-grain ripe earliest or brown rice kind are chosen as parent of future generation;
(4) according to step (1)~(3) repetition many generations.
During conventional rice breeding or rice cropping, the direct presoaking and germinating of rice paddy seed, you can sowed;To paddy rice Dormancy breaking process is carried out, the Seed Germination of Rice time can be shortened, shorten the time that whole seed sows pre-treatment.It is live as water A kind of mode of rice growing, need not carry out Honda transplanting, it is to avoid rice seedling is turned green the spent time, shorten paddy rice and give birth to entirely Educate the phase 10~15 days.In breeding of hybridized rice, after paddy rice enters blooming stage, female parent need to carry out emasculation, and the mode of emasculation is various It is various, for example:Warm water, wet gametocide, cut clever emasculation, chemical emasculation etc..It is of the present invention that to cut clever emasculation be from having lacked The spike of rice that amount is bloomed, takes the top grain husk flower and base portion grain husk flower (the advantage grain husk flower that will be bloomed for second day) of middle and upper part stalk, in Bloom and grain husk is closed after 2 hours, cut clever emasculation, bagging.When cutting grain husk, the 1/3 of clever shell top is cut off, the stamen for exposing is cut off, retained Gynoecium.Pollinated after cutting grain husk.As, by the way of clever emasculation is cut, clever shell is imperfect, the Filling velocity of seed is caused to accelerate, It is i.e. ripe in advance, can generally shorten the breeding time of 10~15 days.
The length of parental rice breeding time has important function for breeding more than a year to realization, short in parental rice breeding time In the case of coordinate said method again, can further shorten breeding process.Preferably, adopting tool photonasty and the time of infertility For the rice varieties of 70~100 days.The rice varieties can both adopt Jing rice, and long-grained nonglutinous rice may also be employed;Can using in East Asia Region, evening Jing rice varieties, may also be employed southern china Indica rice.
Rice yield has certain impact with the length of growth period duration of rice, but still suffers from the paddy rice product that breeding time is short and yield is high Kind, with the rice varieties that parental line selection breeding time is short and yield is high that breeding time is short, the cultivation in generation more than a year can not only be realized, Requirement of the paddy rice product to yield than in can also be met.Preferably, rice varieties survey 56, good 33, good 0664 for good 991, list And good 64.For example:With praise 991 for it is maternal, single survey 56 carry out hybridizing, screen for male parent after the rice varieties that obtain than praising 991 High yield, yield have a big increase.Preferably, the parental rice be combined as good 991 with it is single survey 56, good 33 with good 0664, good 64 with Good 33.
The method of seed Dormancy breaking has various, for example:Advance freezing processing, gibberellin process and dioxygen water process etc., The emphasis point of said method is the germinating energy and germination percentage for improving seed, is sent out for quickly breaking seed dormancy, accelerating seed In terms of bud, using the best results of salpeter solution, therefore, preferably, the method for the Dormancy breaking is to be soaked with aqueous solution of nitric acid Kind.It is further preferred that seed soaking time is 58~60 hours.
During live, the planting density between plant is all had an impact to paddy growth, yield and breeding time length.Plantation The overstocked tiller number for causing plant unit area of density increases, and causes and lodges and disease, and can delay the maturation of paddy rice, Jin Eryan Long growth period duration of rice.Preferably, distance between rows and hills during direct sowing of rice is 8cm × 8cm.
During crossbreeding, the only synchronous cultivation of guaranteed maternal, male parent maximized could realize Dai Yu more than a year Kind;Female parent is cut clever emasculation process and can shorten its pustulation period, therefore, male parent should also carry out cutting grain husk.Therefore, in step (2), broadcast by adjusting Phase, it is ensured that male parent enters blooming stage simultaneously with maternal, cuts grain husk to male parent.
Treat that paddy rice enters the grouting later stage, the seed in each tiller of paddy rice starts maturation, but maturation time is different, and when ripe Between be spaced that gap is larger, select ripe earliest a part of seed-grain or brown rice kind to carry out subsequent seed process, then directly sowing, The time can be shortened.During blooming stage, rice glume is cut, and when causing Grain Ripening, part seed husk comes off, and is formed Brown rice.Brown rice is more easy to Dormancy breaking and quick pregermination, spends the time shorter.
However, from the beginning of threshing is carried out after paddy rice maturation, pre-process to seed-grain or brown rice kind, then to sowing, period needs Spend additional time, during easily causing two generation Rice Croppings, the neutral time occur, if shortening the neutral time, it is also possible to Shorten the Rice Cropping holocyclic time.Preferably, in step (3), choosing spike of rice ripe earliest as follow-on parent This.Directly take spike of rice to be dried, a stalk can be made to become fragile, in follow-up Dormancy breaking and pregermination procedure, directly can be come off, Eliminate the process of threshing.In corn-cob picking procedure, part brown rice can be split away off from rice husk, which can be together broken with seed-grain Except dormancy and vernalization, and carry out live.
Preferably, the water content of the rice paddy seed is less than≤13%, when vernalization to the broken chest rate of seed is not less than 80% Carry out live.
Realize more than a year in breeding, select suitable breeding place, that is, select high temperature, short-day regions, for photosensitive Property paddy rice can substantially shorten its time of infertility.In China, Hainan is the ground of the optimal selection of crop breeding, according to the weather in Hainan Feature, selects the date of seeding being adapted to, can further coordinate the inventive method, to shorten breeding cycle.
Compared with prior art, the invention has the advantages that:
The present invention adopts direct sowing culture, cuts clever emasculation, brown rice vernalization sowing, and salpeter solution seed soaking is quickly broken seed and stopped Dormancy, the breeding cultivation mode for accelerating seed sprouting, shorten the time of infertility of paddy rice, and then shorten breeding process, realize paddy rice More than a year for breeding, in solving the problems, such as prior art, breeding cycle is long.
Specific embodiment
With reference to specific embodiment, the invention will be further elaborated.
What is do not elaborated in the following example rice breeding mode is routine techniques means.
In 1 one years five generations of embodiment, breed
First, test place:Our department of Jiaxing City academy of agricultural sciences experimental plot and Hainan Lingshui county Ye Lin towns Nanling breeding base
2nd, experiment material:Naked body evening Jing rice praises good 33 (male parents) of 64 (female parents), the non-smooth leaf kind of early season late-rice
3rd, date of seeding:The first season:On May 25th, 2009 sows (first season, remaining was in Hainan in Jiaxing);The second season:2009 On September sowing in 22,;The third quarter:On November 26th, 2009 is sowed;The fourth season:Sow within 2 23rd, 2010;5th season:2010 The upper ten days sowing of May in year.
4th, breeding method
(1) water intaking rice parental seed, is placed in aqueous solution of nitric acid and soaks seed 58 hours, thoroughly eliminates seed dormancy, using clear After water wash 3~5 times, vernalization in 30 DEG C of insulating box is put into, to the 80% broken chest of seed, is carried out live.
The preparation method of aqueous solution of nitric acid is:Measured with graduated cylinder, add 3.5mL, the pure dense nitre of 36% analysis by 1000mL clear water The proportions of sour stoste.
Live broadcasting method is:The small sample material of sowing is taken, and furrow is done by the specification line of seedling plate width 110cm, furrow width 30cm Smooth seedling plate, is divided into two seedling plate, marks sowing division by 50cm, and every part of material carries out program request by the distance between rows and hills of 8 × 8cm specifications 50, it is desirable to which distance between rows and hills is uniform.
During paddy rice tri-leaf period, carry out pouring water, heavy dressing fertilizer promotees paddy rice and early sends out.The live material of big colony is before tiller starts Carry out deleting close thinning, it is ensured that each individual plant independent growths of segregating generation, be easy to Single-plant selection and detection.The short day property of high temperature is stronger Middle Jing or evening Jing, particularly autumn, spring temperature higher season add generation, well-developed individuality, 5~6 leaf phases just can start children's fringe Differentiation, starts heading for more than 40 days, preferably imposes a fertilizer in tillering stage, do not refertilize afterwards, strong, precocious with health care, ears whole Neat non-lodging.
(2) treat that paddy rice enters blooming stage, carry out crossover operation, clever emasculation, pollination operation are cut to female parent;
Cutting clever emasculation with the detailed process pollinated is:Paddy rice enters blooming stage, after the spike of rice bloomed on a small quantity to appear, in taking The top grain husk flower of top stalk and base portion grain husk spend 30, the maternal tassel chosen first is carried out whole fringe with scissors, then, has been opened Flower and the grain husk flower that will not be bloomed in 2~3 days are cut off with little scissors, only retained ripe but still unopened grain husk flower, then by clever shell End 1/3-1/4 cuts, then with tweezers folder except stamen.It is maternal consistent with male parent breeding time to ensure, using said method to father Originally carry out cutting clever emasculation.
(3) to grouting parameter, spike of rice (i.e. second generation seed) ripe earliest is chosen, threshing is not carried out, is placed directly within Rapid draing in 50 DEG C of electrically heated drying cabinet is less than or equal to 13% to seed moisture content in 24 hours, then is placed on aqueous solution of nitric acid In (compound method is ibid), seed soaking 60 hours, break seed dormancy, and after clear water drip washing, 30 DEG C of constant temperature vernalization are broken to 80% seed After chest, sowed.
During due to blooming stage, clever shell can be cut off a part, so, spike of rice drying, Dormancy breaking, vernalization process In, part seed can come off from clever shell, as brown rice.Brown rice is identical with the processing method of the seed-grain not fallen off.
(4) according to step (1)~(3) repetition many generations.
1 naked body rice of table it is good 64 it is near etc. be Establishing process
Explanation:P1It is early season late-rice naked body rice good 64, P2It is the non-smooth leaf kind of early season late-rice good 33, by Jiangsu, Zhejiang River and Shanghai authorization.
The success of the test realizes the breeding process in 1 year continuous 5 season.Table 1 is the good 64 smooth phyllopodium of naked body evening Jing rice because near etc. The quick process set up of system, from the F using the single evening plantation in Jiaxing on the 1st of September in 20091(naked body rice good 64 and high yield commercial variety Good 33 hybridization), continue and good 33 backcrossing, September sows B in Lingshui county of Hainan Province Ye Lin towns south gate ridge breeding base on 22nd1F1Extremely In continuous 3 generation of fast breeding in 213 days of on April 20th, 2010, in 71 days average 1 generations, in the 4th generation, is in 4/23 together with P2Continue sowing Plus generation, the breeding time into summer and autumn paddy rice can also shorten more, with above-mentioned mating system, be expected within 1 year to complete for 5~6 seasons.
In 2 one years four generations of embodiment, breed
First, test place:Our department of Jiaxing City academy of agricultural sciences experimental plot and Hainan Lingshui county Ye Lin towns Nanling breeding base
2nd, experiment material:Naked body evening Jing rice praises good 33 (female parents) of 0664 (male parent), early season late-rice kind
3rd, date of seeding:The first season:The sowing of Hainan at the beginning of 10 months in 2006;The second season:Hainan sowing of on December 30th, 2006;The Three seasons:Hainan sowing of on March 20th, 2007;The fourth season:Jiaxing sowing of on June 10th, 2007.
4th, breeding method
(1) water intaking rice parental seed, is placed in aqueous solution of nitric acid and soaks seed 60 hours, thoroughly eliminates seed dormancy, using clear After water wash 3~5 times, vernalization in 30 DEG C of insulating box is put into, to the 80% broken chest of seed, is carried out live.
The preparation method of aqueous solution of nitric acid is:Measured with graduated cylinder, it is 36% to add 2.5mL, mass fraction by 1000mL clear water Analyze the proportions of pure red fuming nitric acid (RFNA) stoste.
Live broadcasting method is:The small sample material of sowing is taken, and furrow is done by the specification line of seedling plate width 180cm, furrow width 35cm Smooth seedling plate, is divided into two seedling plate, marks sowing division per 50cm, carries out program request 50 by the distance between rows and hills of 8 × 8cm specifications and goes forward side by side Row light seeding is cultivated.
During paddy rice tri-leaf period, carry out pouring water, heavy dressing fertilizer promotees paddy rice and early sends out.The live material of big colony is before tiller starts Carry out deleting close thinning, it is ensured that each individual plant independent growths of segregating generation, be easy to Single-plant selection and detection.The short day property of high temperature is stronger Middle Jing or evening Jing, particularly autumn, spring temperature higher season add generation, well-developed individuality, 5~6 leaf phases just can start children's fringe Differentiation, starts heading for more than 40 days, preferably imposes a fertilizer in tillering stage, do not refertilize afterwards, strong, precocious with health care, ears whole Neat non-lodging.
(2) treat that paddy rice enters blooming stage, carry out crossover operation, clever emasculation, pollination operation are cut to female parent;
Cutting clever emasculation with the detailed process pollinated is:Paddy rice enters blooming stage, after the spike of rice bloomed on a small quantity to appear, in taking The top grain husk flower of top stalk and base portion grain husk spend 25, the maternal tassel chosen first is carried out whole fringe with scissors, then, has been opened Flower and the grain husk flower that will not be bloomed in 2~3 days are cut off with little scissors, only retained ripe but still unopened grain husk flower, then by clever shell End 1/3-1/4 cuts, then with tweezers folder except stamen.It is maternal consistent with male parent breeding time to ensure, using said method to father Originally carry out cutting clever emasculation.
(3) to grouting parameter, spike of rice (i.e. second generation seed) ripe earliest is chosen, threshing is not carried out, is placed directly within Rapid draing in 50 DEG C of electrically heated drying cabinet is less than or equal to 13% to seed moisture content in 24 hours, then is placed on aqueous solution of nitric acid In (compound method is ibid), seed soaking 60 hours, break seed dormancy, and after clear water drip washing, 30 DEG C of constant temperature vernalization are broken to 80% seed After chest, sowed.
During due to blooming stage, clever shell can be cut off a part, so, spike of rice drying, Dormancy breaking, vernalization process In, part seed can come off from clever shell, as brown rice.Brown rice is identical with the processing method of the seed-grain not fallen off.
(4) according to step (1)~(3) repetition many generations.
The success of the test realizes the breeding process in 1 year 4 generation.33 are praised at the beginning of in the September, 2006 of Jiaxing with good 0664 hybridization; Fine jade F at the beginning of 10 months in 20061Live plus generation;The fine jade F of on December 30th, 20062In live plus generation, select 200 single fringes;March 20 in 2007 Day fine jade F3Head progeny row broadcasts continuation plus generation, June 10 the same year Jiaxing F4False single cropping direct sowing culture, is so capable of achieving breeding for 1 year four generation.
Embodiment 3 one years three generations breed
First, test place:Our department of Jiaxing City academy of agricultural sciences experimental plot and Hainan Lingshui county Ye Lin towns Nanling breeding base
2nd, experiment material:High-quality evening Jing rice varieties praise 991 (female parents), and big fringe high yield evening Jing strains list of short stem surveys 56 (fathers This)
3rd, date of seeding:Jiaxing hybridization of autumn in 1999, in October, 1999 Hainan F1Sowing, in December, 1999 Hainan F2Sowing, In May, 2000 Jiaxing F3Sowing, in October, 2000 Hainan F4Sowing, in December, 2000 Hainan F5Sowing, May calendar year 2001 Jiaxing F6 Sowing, finalizes the design almost.
4th, breeding method
(1) water intaking rice parental seed, is placed in aqueous solution of nitric acid and soaks seed 58 hours, thoroughly eliminates seed dormancy, using clear After water wash 3~5 times, vernalization in 30 DEG C of insulating box is put into, to the 80% broken chest of seed, is carried out live.
The preparation method of aqueous solution of nitric acid is:Measured with graduated cylinder, add 3.5mL, the pure dense nitre of 36% analysis by 1000mL clear water The proportions of sour stoste.
Live broadcasting method is:The small sample material of sowing is taken, and furrow is done by the specification line of seedling plate width 110cm, furrow width 30cm Smooth seedling plate, is divided into two seedling plate, marks sowing division by 50cm, and every part of material carries out program request by the distance between rows and hills of 8 × 8cm specifications 50, it is desirable to which distance between rows and hills is uniform.
During paddy rice tri-leaf period, carry out pouring water, heavy dressing fertilizer promotees paddy rice and early sends out.The live material of big colony is before tiller starts Carry out deleting close thinning, it is ensured that each individual plant independent growths of segregating generation, be easy to Single-plant selection and detection.The short day property of high temperature is stronger Middle Jing or evening Jing, particularly autumn, spring temperature higher season add generation, well-developed individuality, 5~6 leaf phases just can start children's fringe Differentiation, starts heading for more than 40 days, preferably imposes a fertilizer in tillering stage, do not refertilize afterwards, strong, precocious with health care, ears whole Neat non-lodging.
(2) treat that paddy rice enters blooming stage, carry out crossover operation, clever emasculation, pollination operation are cut to female parent;
Cutting clever emasculation with the detailed process pollinated is:Paddy rice enters blooming stage, after the spike of rice bloomed on a small quantity to appear, in taking The top grain husk flower of top stalk and base portion grain husk spend 30, the maternal tassel chosen first are carried out whole fringe with scissors, then, by Bloom with 2~3 days in the grain husk flower that will not bloom cut off with little scissors, only retained ripe but still unopened grain husk flower, then by clever shell Upper end 1/3-1/4 cuts, then with tweezers folder except stamen.It is maternal consistent with male parent breeding time to ensure, using said method pair Male parent carries out cutting clever emasculation.
(3) to grouting parameter, spike of rice (i.e. second generation seed) ripe earliest is chosen, threshing is not carried out, is placed directly within Rapid draing in 50 DEG C of electrically heated drying cabinet is less than or equal to 13% to seed moisture content in 24 hours, then is placed on aqueous solution of nitric acid In (compound method is ibid), seed soaking 60 hours, break seed dormancy, and after clear water drip washing, 30 DEG C of constant temperature vernalization are broken to 80% seed After chest, sowed.
During due to blooming stage, clever shell can be cut off a part, so, spike of rice drying, Dormancy breaking, vernalization process In, part seed can come off from clever shell, as brown rice.Brown rice is identical with the processing method of the seed-grain not fallen off.
The success of the test realizes the breeding process in 1 year 3 generation.Qiu Jiaxingjia 991 in 1999 is hybridized with single survey 56, and 1999 Year October Hainan F1Live sowing, in December, 1999 Hainan F2Plus generation, in May, 2000 Jiaxing F3Plus generation, resistance, quality-screening, In October, 2000 Hainan F4Plus generation, resistance, quality, low temperature resistant screening, in December, 2000 Hainan F5Plus for resistance, quality-screening, May calendar year 2001 Jiaxing F6In generation, finalizes the design almost, and is so capable of achieving breeding for 1 year 3 generation.

Claims (5)

1. paddy rice is more than a year for breeding method, it is characterised in that comprise the following steps:
(1) fetch water rice, carry out after Dormancy breaking, vernalization it is live, distance between rows and hills when live be 8cm × 8cm;
Wherein, rice varieties survey 56, good 33, good 0664 and good 64 for good 991, list;Parent's is combined as good 991 with single survey 56th, 33 are praised with good 0664, good 64 and good 33;
(2) by adjusting date of seeding, male parent is made with female parent while entering blooming stage, treat that paddy rice enters blooming stage, grain husk is cut to male parent, and Female parent is carried out cutting clever emasculation, pollination operation;
(3) to grouting parameter, seed-grain ripe earliest or brown rice kind are chosen as parent of future generation;
(4) according to step (1)~(3) repetition many generations.
2. rice breeding method as claimed in claim 1, it is characterised in that the method for the Dormancy breaking is water-soluble with nitric acid Immersion kind.
3. rice breeding method as claimed in claim 1, it is characterised in that in step (3), chooses spike of rice ripe earliest and makees For follow-on parent.
4. rice breeding method as claimed in claim 1, it is characterised in that the water content of the rice paddy seed less than≤ 13%.
5. rice breeding method as claimed in claim 1, it is characterised in that vernalization to seed broken chest rate is entered when being not less than 80% Row is live.
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CN106577264A (en) * 2016-12-21 2017-04-26 漯河市农业科学院 Corn inbred material homozygosis breeding method for Huang-Huai-Hai areas
CN106857236A (en) * 2016-12-28 2017-06-20 陵水重学辐射育种研究所 A kind of method that quick subculture of paddy rice is bred
CN113692804A (en) * 2021-10-08 2021-11-26 江苏千重浪农业科技发展有限公司 Breeding method of high-quality high-yield disease-resistant rice variety
CN116267470B (en) * 2023-03-29 2024-04-19 江苏省农业科学院 Method for promoting rapid generation adding of rice

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1262031A (en) * 1999-02-01 2000-08-09 河北省农林科学院农业物理生理生化研究所 High-speed plant breeding technique by more generations in one year
CN103430829A (en) * 2013-08-14 2013-12-11 江苏丘陵地区镇江农业科学研究所 Efficient japonica rice breeding method

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007228914A (en) * 2006-03-02 2007-09-13 Tokyo Univ Of Science Low temperature-resistant plant and method for producing and identifying the same
CN103125391B (en) * 2013-03-07 2015-06-17 河北农业大学 Local multi-generation breeding method for soybean

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1262031A (en) * 1999-02-01 2000-08-09 河北省农林科学院农业物理生理生化研究所 High-speed plant breeding technique by more generations in one year
CN103430829A (en) * 2013-08-14 2013-12-11 江苏丘陵地区镇江农业科学研究所 Efficient japonica rice breeding method

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
光身软香米新品种嘉58特征特性及栽培技术;高荣村等;《中国稻米》;20141231;第20卷(第2期);第74-75页 *

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