CN104838908A - Method for inhibiting growth and development of tomato lateral branches - Google Patents
Method for inhibiting growth and development of tomato lateral branches Download PDFInfo
- Publication number
- CN104838908A CN104838908A CN201510214785.7A CN201510214785A CN104838908A CN 104838908 A CN104838908 A CN 104838908A CN 201510214785 A CN201510214785 A CN 201510214785A CN 104838908 A CN104838908 A CN 104838908A
- Authority
- CN
- China
- Prior art keywords
- tomato
- concentration
- growth
- plant
- oll
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 235000007688 Lycopersicon esculentum Nutrition 0.000 title claims abstract description 49
- 238000000034 method Methods 0.000 title claims abstract description 41
- 230000012010 growth Effects 0.000 title abstract description 16
- 238000011161 development Methods 0.000 title abstract description 6
- 230000002401 inhibitory effect Effects 0.000 title abstract description 4
- 240000003768 Solanum lycopersicum Species 0.000 title description 48
- 239000000243 solution Substances 0.000 claims abstract description 18
- 238000002347 injection Methods 0.000 claims abstract description 10
- 239000007924 injection Substances 0.000 claims abstract description 10
- 241000227653 Lycopersicon Species 0.000 claims abstract 14
- 150000002596 lactones Chemical class 0.000 claims description 28
- 230000008569 process Effects 0.000 claims description 23
- 241000208000 Striga Species 0.000 claims description 18
- UUTKICFRNVKFRG-WDSKDSINSA-N (4R)-3-[oxo-[(2S)-5-oxo-2-pyrrolidinyl]methyl]-4-thiazolidinecarboxylic acid Chemical compound OC(=O)[C@@H]1CSCN1C(=O)[C@H]1NC(=O)CC1 UUTKICFRNVKFRG-WDSKDSINSA-N 0.000 claims description 4
- 238000007654 immersion Methods 0.000 claims description 4
- 238000010899 nucleation Methods 0.000 claims 1
- 241000196324 Embryophyta Species 0.000 abstract description 18
- XHSDUVBUZOUAOQ-WJQMYRPNSA-N (3e,3ar,8bs)-3-[[(2r)-4-methyl-5-oxo-2h-furan-2-yl]oxymethylidene]-4,8b-dihydro-3ah-indeno[1,2-b]furan-2-one Chemical compound O1C(=O)C(C)=C[C@@H]1O\C=C/1C(=O)O[C@@H]2C3=CC=CC=C3C[C@@H]2\1 XHSDUVBUZOUAOQ-WJQMYRPNSA-N 0.000 abstract description 13
- 241000238631 Hexapoda Species 0.000 abstract description 4
- 201000010099 disease Diseases 0.000 abstract description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 4
- 230000008635 plant growth Effects 0.000 abstract description 2
- 241000607479 Yersinia pestis Species 0.000 abstract 1
- 239000004480 active ingredient Substances 0.000 abstract 1
- 210000001099 axilla Anatomy 0.000 abstract 1
- 239000003054 catalyst Substances 0.000 abstract 1
- 238000002791 soaking Methods 0.000 abstract 1
- 238000012360 testing method Methods 0.000 description 11
- 230000000694 effects Effects 0.000 description 7
- 239000000463 material Substances 0.000 description 6
- 238000004904 shortening Methods 0.000 description 5
- 235000013311 vegetables Nutrition 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 4
- 239000003375 plant hormone Substances 0.000 description 4
- 238000004458 analytical method Methods 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 208000037805 labour Diseases 0.000 description 3
- 230000001105 regulatory effect Effects 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 239000010931 gold Substances 0.000 description 2
- 229910052737 gold Inorganic materials 0.000 description 2
- -1 gold lactone Chemical class 0.000 description 2
- 239000005556 hormone Substances 0.000 description 2
- 229940088597 hormone Drugs 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 241000219194 Arabidopsis Species 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 240000007377 Petunia x hybrida Species 0.000 description 1
- 240000004713 Pisum sativum Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000003750 conditioning effect Effects 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 238000009313 farming Methods 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 238000013138 pruning Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 235000020046 sherry Nutrition 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 230000026676 system process Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a method for inhibiting the growth and development of lateral branches of tomatoes. The concentration of the catalyst is 7.5 to 10.0 mu mol/L-1Exogenous smearing treatment is carried out on tomato plants by the strigolactone solution; or the concentration of the active ingredient is 2.5 to 5.0. mu. mol. L-1The strigolactone solution is used for carrying out exogenous injection treatment on tomato plants; or the concentration is 10.0 to 20.0 mu mol/L-1The strigolactone solution is used for soaking the root systems of the tomato seedlings for 20-24 hours 1-2 days before the seedlings are planted. Exogenous smearing and injection treatment are carried out at the axilla of the leaf of the branch part of the main stem of the tomato, and the dosage is 10 mu L per plant. The method obviously inhibits the growth and development of lateral branches of tomatoes, reduces labor consumption and disease and insect pest propagation, promotes the growth of plants and improves the yield and quality, has the characteristics of simple operation and no pollution, and is suitable for the cultivation of outdoor tomatoes and facility tomatoes.
Description
Technical field
The present invention relates to a kind of method of regulating growth of plants, particularly a kind of method suppressing tomato collateral generation to be grown by plant hormone, belongs to technical field of vegetable cultivation.
Background technology
Tomato, also referred to as tomato, tomato, originates in the tropical highlands of the Peru and Ecuador in western part, America.In recent years, along with the rapid expansion of China's facilities vegetable area, tomato cultivation area constantly increases, and has become one of main cultivation vegetable variety of China, increases played important effect to promotion increasing peasant income and social economy.Tomato plant strain growth gesture is strong, and each lateral bud can be sprouted formation side shoot, yield positive results.Particularly after plant pinching, because the transfer at nutrition center can sprout more side shoot usually; The sprouting of side shoot and growth can consume a large amount of nutriments, and due to branches and leaves overstocked, ventilation and penetrating light be deteriorated, damage by disease and insect increases the weight of, and causes tomato yield to reduce.Research shows, can plant lateral meristem form axillalry bud and grow formation branch further and be subject to plant hormone, environment and genic regulation and control, and wherein plant hormone plays key effect in regulating plant branch growth course.In recent years, by to a series of multi-branched mutant, comprise the research of the mutant such as pea, arabidopsis, petunia, paddy rice, demonstrate witchweed lactone and can regulate and control collateral development as the plant hormone that a class is new, in plant corpus, solely the amount of angle gold lactone reduces, branch increases, and external only angle gold lactone of using then can change the mutant phenotype of plant.
In tomato cultivation process of growth, in order to control the growth of tomato side shoot, vegetable grower needs regularly to carry out operation adjustment plant strain growths such as pruning, prune, and the operation of these farming consumes a large amount of manpowers and time, and labour intensity is large, and production cost is high.For this reason, find a kind of method being regulated and controled and change tomato collateral generation by exogenous hormone and grow, change plant strain growth habit, for reduction damage by disease and insect, reduce labor intensity, improve output, improve effects has realistic meaning.
Summary of the invention
The object of this invention is to provide a kind of method suppressing tomato collateral generation to be grown, adopt the external source of witchweed lactone solution to smear, the modes such as external source injection or root system process process tomato plant, better suppress tomato collateral generation developmental capacity to obtain.
The technical scheme that the present invention takes is such:
Suppressing the method that tomato collateral generation is grown, is 7.5 ~ 10.0 μm of olL by concentration
-1witchweed lactone solution external source carried out to tomato plant smear process;
Or be 2.5 ~ 5.0 μm of olL by concentration
-1witchweed lactone solution external source injection treatment is carried out to tomato plant;
Or be 10.0 ~ 20.0 μm of olL by concentration
-1witchweed lactone solution tomato seedling root system is processed.
In the method for the invention, for obtaining the effect better suppressing tomato collateral generation to be grown, it is adopt at the axil place at tomato plant stem branch position to smear and inject witchweed lactone solution that external source is smeared with external source injecting method, and consumption is 10 μ L/ strains.
In the method for the invention, be before field planting 1 ~ 2 day to the process of tomato seedling root system, adopt witchweed lactone solution root immersion 20 ~ 24 hours.
Test confirms, by carrying out the process of aforesaid way to tomato plant or seedling, can find that growing of tomato side shoot is significantly inhibited, be conducive to tomato late growing stage.
Method of the present invention is applicable to the cultivation of open country tomato and greenhouse tomato.
The invention has the beneficial effects as follows: the present invention has following advantage compared with existing artificial plant method of adjustment: the growth 1, reducing side shoot, save and tomato plant is pruned the required a large amount of labours that prune, and decrease the propagation of damage by disease and insect; 2, tomato plant collateral development is suppressed, and decreases the loss of nutrient, is conducive to the raising of plant strain growth and yield and quality; 3, compare relative to other conditioning agents, witchweed lactone is as a kind of hormone, and to human body without any harm, and after process crop, cultivating soil, matrix do not exist residual; 4, processing method is easy, is easy to operation.
Embodiment
Below by way of instantiation, the present invention will be described further, but do not limit the scope of the invention.
Embodiment 1 smears the effect test that witchweed lactone suppresses tomato collateral generation to be grown
1, test material, experimental scheme
Test material is tomato ' in assorted No. 9 ' (seed is provided by Vegetable & Flower Inst., Chinese Academy of Agriculture Science), and witchweed lactone used is the Prof. Du Yucang analog GR24(purity 99.9% purchased from industrial Science and Technology Ltd. of Beijing Qun Dynasty).
Tomato seedling is colonizated in greenhouse by solar heat, manages according to a conventional method.When plant grows to 80 ~ 90cm, choose the strain of growing way consistent plant 30 and (be divided into 3 groups, often organize 10 strains), at the axil place at tomato stem branch position, (mark is first segment position herein, and up increase progressively successively) smear witchweed lactone (GR24) solution, process as follows: (1) with the plant of normal growth for contrast (CK); (2) 7.5 μm of olL are smeared
-1gR24; (3) 10 μm of olL are smeared
-1gR24.GR24 consumption is 10 μ L/ strains.Process after 25 days, measure the length of plant process joint position and above joint position side shoot.
2, test results and analysis
As can be seen from Table 1, compared with control treatment, smear 7.5 μm of olL at tomato stem branch position
-1after GR24, the lateral bud of tomato first segment position has no sprouting, and the growth of second section position, Section of three position side shoot is significantly inhibited, and side shoot length is respectively than contrast shortening 55.1% and 52.1%; It is 10 μm of olL that GR24 smears concentration
-1time, its inhibitory action is than 7.5 μm of olL
-1more remarkable, first segment position and second section position are showed no sprouting of lateral bud, and Section of three position collateral generation is subject to extremely significantly suppressing, and length is only 3.0% of contrast.
The impact of witchweed lactone on tomato collateral generation smeared by table 1
Table in different lowercase (a, b, c) represent process between the significance of difference, p<0.05.
Embodiment 2 injects the effect that witchweed lactone suppresses tomato collateral generation to be grown
1, test material, experimental scheme
Test material be tomato ' like lucky 112 ' (seed is provided by Jiangsu Lv Gang modern agricultural development limited company), witchweed lactone used is the Prof. Du Yucang analog GR24(purity 99.9% purchased from industrial Science and Technology Ltd. of Beijing Qun Dynasty).
Tomato seedling is colonizated in greenhouse by solar heat, manages according to a conventional method.When plant grows to 80-90cm, choose the strain of growing way consistent plant 30 and (be divided into 3 groups, often organize 10 strains), at the axil place at tomato stem branch position, (mark is first segment position herein, and up increase progressively successively) injection witchweed lactone (GR24) solution, process as follows: (1) with the plant of normal growth for contrasting (CK); (2) 2.5 μm of olL are injected
-1gR24; (3) 5 μm of olL are injected
-1gR24.Injection GR consumption is 10 μ L/ strains.Process after 25 days, measure the length of plant process joint position and above joint position side shoot.
2, test results and analysis
As can be seen from Table 2, compared with control treatment, be 2.5 μm of olL at tomato stem branch position injection concentration
-1gR24 process, the lateral bud of tomato first segment position has no sprouting, and the growth of second section position, Section of three position side shoot is significantly inhibited, and side shoot length respectively than contrast shortening 35.1% and 24.5%; Injection concentration is 5 μm of olL
-1gR24 time, its inhibitory action is more obvious, and the length of Section three position side shoot is than contrasting shortening 78.9%.
the impact that witchweed lactone is grown tomato collateral generation injected by table 2
Table in different lowercase (a, b, c) represent process between the significance of difference, p<0.05.
The process of embodiment 3 witchweed lactone root immersion suppresses the effect of tomato collateral generation
1, test material, experimental scheme
Test material is tomato ' sherry ' (seed is provided by this prosperous (China) seed Co., Ltd of Rake), and witchweed lactone used is the Prof. Du Yucang analog GR24(purity 99.9% purchased from industrial Science and Technology Ltd. of Beijing Qun Dynasty).
Adopt point disk seedling growing method to carry out tomato seedling cultivation, treat that seedling grows 4-5 sheet true leaf and can carry out field planting transplanting.1-2 days before field planting, chooses the seedling (be divided into 4 groups, often organize 10) of 40 strain growing way neat and consistent, after cleaning root, is 10 μm of olL respectively by concentration with clear water
-1, 20 μm of olL
-1witchweed lactone (GR24) solution root immersion, soak time is 24h.With distilled water process in contrast (CK).Transplant after 60 days, the length of measurement first segment position and above each joint position side shoot.
2, test results and analysis
As can be seen from Table 3, along with the raising of GR24 concentration, more obvious to the inhibition of tomato collateral generation: 10 μm of olL
-1gR24 process, first segment position and second section position side shoot have no sprouting, and Section of three position is significantly inhibited to Section of six position side shoot length, respectively than contrast shortening 78.2%, 85.3%, 65.9%, 93.9%; 20 μm of olL
-1gR24 process, first segment position has no sprouting to Section of four position side shoot, and Section of five position and Section of six position side shoot length are significantly inhibited, respectively than contrast shortening 95.1% and 97.2%.
The impact that table 3 witchweed lactone solution process root system is grown tomato collateral generation
Table in different lowercase (a, b, c) represent process between the significance of difference, p<0.05.
It is noted that above-described embodiment is only for explaining the present invention, do not form limiting the scope of the present invention.Although with reference to above-described embodiment to invention has been detailed description, those of ordinary skill in the art still can modify to the present invention or replace on an equal basis, and not departing from any modification or partial replacement of the spirit and scope of the present invention, it all should be encompassed in the middle of right of the present invention.
Claims (3)
1. the method suppressing tomato collateral generation to be grown, is characterized in that: be 7.5 ~ 10.0 μm of olL by concentration
-1witchweed lactone solution external source carried out to tomato plant smear process; Or be 2.5 ~ 5.0 μm of olL by concentration
-1witchweed lactone solution external source injection treatment is carried out to tomato plant; Or be 10.0 ~ 20.0 μm of olL by concentration
-1witchweed lactone solution tomato seedling root system is processed.
2. method according to claim 1, is characterized in that: it is adopt at the axil place at tomato plant stem branch position to smear and inject witchweed lactone solution that external source is smeared with external source injection treatment, and consumption is 10 μ L/ strains.
3. method according to claim 1, is characterized in that: carrying out process to tomato seedling root system is before Seeding planting 1 ~ 2 day, adopts witchweed lactone solution root immersion 20 ~ 24 hours.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510214785.7A CN104838908B (en) | 2015-04-30 | 2015-04-30 | Method for inhibiting growth and development of tomato lateral branches |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510214785.7A CN104838908B (en) | 2015-04-30 | 2015-04-30 | Method for inhibiting growth and development of tomato lateral branches |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104838908A true CN104838908A (en) | 2015-08-19 |
| CN104838908B CN104838908B (en) | 2017-12-19 |
Family
ID=53839225
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510214785.7A Expired - Fee Related CN104838908B (en) | 2015-04-30 | 2015-04-30 | Method for inhibiting growth and development of tomato lateral branches |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104838908B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107211853A (en) * | 2017-05-26 | 2017-09-29 | 中国农业科学院油料作物研究所 | A kind of method for studying the golden lactone regulation and control rape root growth in only angle |
| CN112005834A (en) * | 2020-07-06 | 2020-12-01 | 佛山科学技术学院 | Method for promoting growth of peas and increasing yield of peas |
| CN116491328A (en) * | 2023-05-29 | 2023-07-28 | 华南农业大学 | Method for inhibiting axillary bud of glutinous rice cake litchi from light extraction and application |
| CN116897961A (en) * | 2023-07-20 | 2023-10-20 | 仲恺农业工程学院 | Plant branching regulator and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104349675A (en) * | 2012-05-24 | 2015-02-11 | 先正达参股股份有限公司 | Plant growth regulating compounds |
-
2015
- 2015-04-30 CN CN201510214785.7A patent/CN104838908B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104349675A (en) * | 2012-05-24 | 2015-02-11 | 先正达参股股份有限公司 | Plant growth regulating compounds |
Non-Patent Citations (2)
| Title |
|---|
| 冯丹等: "独脚金内酯调控侧枝发育的研究进展", 《生态学杂志》 * |
| 刘拥海等: "植物激素对分枝发育的协同调控作用研究进展", 《植物生理学报》 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107211853A (en) * | 2017-05-26 | 2017-09-29 | 中国农业科学院油料作物研究所 | A kind of method for studying the golden lactone regulation and control rape root growth in only angle |
| CN107211853B (en) * | 2017-05-26 | 2019-11-15 | 中国农业科学院油料作物研究所 | A method of research witchweed lactone regulation rape root growth |
| CN112005834A (en) * | 2020-07-06 | 2020-12-01 | 佛山科学技术学院 | Method for promoting growth of peas and increasing yield of peas |
| CN116491328A (en) * | 2023-05-29 | 2023-07-28 | 华南农业大学 | Method for inhibiting axillary bud of glutinous rice cake litchi from light extraction and application |
| CN116491328B (en) * | 2023-05-29 | 2024-06-11 | 华南农业大学 | Method for inhibiting axillary bud of glutinous rice cake litchi from light extraction and application |
| CN116897961A (en) * | 2023-07-20 | 2023-10-20 | 仲恺农业工程学院 | Plant branching regulator and application thereof |
| CN116897961B (en) * | 2023-07-20 | 2024-03-22 | 仲恺农业工程学院 | Plant branching regulator and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104838908B (en) | 2017-12-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110692475B (en) | Cultivation method for promoting early flowering and early maturing of peanuts | |
| CN107087502B (en) | Method for cultivating double-sprout-tiller grafted seedlings of tomatoes | |
| Bag et al. | Potato (Solanum tuberosum) aeroponics for quality seed production in north eastern Himalayan region of India | |
| CN104838908B (en) | Method for inhibiting growth and development of tomato lateral branches | |
| Abbaszadeh et al. | An effective nutrient media for asymbiotic seed germination and in vitro seedling development of phalaenopsis ‘Bahia Blanca’ | |
| CN102771307A (en) | Method for transplanting sprout stocks of zelkova serrata | |
| CN105940901B (en) | A method of delay B. campestris L.ssp. Chinensis bolting | |
| CN106973796A (en) | A kind of tissue cultivating and seedling method of Idesia polycarpa | |
| CN104322370A (en) | Bitter gourd in vitro rapid propagation method | |
| CN111685127B (en) | Inducer for promoting vegetative propagation of plants and application thereof | |
| CN106171996B (en) | A kind of rapid propagation method of wild white birch | |
| CN110199884B (en) | Selenegia quinoa tissue culture seedling seed setting and cultivating method under aseptic condition | |
| CN112166928A (en) | Method for adjusting custard apple producing period | |
| CN112119913A (en) | Virus-free propagation method for sweet potatoes | |
| CN104303765B (en) | The high-yield planting method of the stem of noble dendrobium | |
| CN101564010B (en) | Method for rapidly propagating tupelos | |
| CN104160856B (en) | A kind of cultivation method improving kale cut-flower amount and production of hybrid seeds amount | |
| CN106489477B (en) | A kind of Ecology Chinese podophyllum root method for culturing seedlings | |
| CN109463065A (en) | A method of promoting wild flex seed fast-germination | |
| CN106922397B (en) | Method for cultivating easily-branched poinsettia | |
| CN105145096A (en) | Nightshade cultivation method | |
| MIGLIOLI et al. | Cytokinin-regulated physiological parameters affected by an exogenous dopamine spray in Brussels sprout (Brassica oleracea var. gemmifera) | |
| CN107593448A (en) | A kind of method that rapid propagation in vitro is carried out using eight treasures (choice ingredients of certain special dishes) red-spotted stonecrop aseptic seedling stem sections | |
| CN108464207B (en) | Culture method for improving transplanting survival rate of rice tissue culture seedlings | |
| CN102550357A (en) | Method for seeding and cultivating breeding plants after apricot harvest in same year |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| EXSB | Decision made by sipo to initiate substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20171219 |