CN112005834A

CN112005834A - Method for promoting growth of peas and increasing yield of peas

Info

Publication number: CN112005834A
Application number: CN202010640817.0A
Authority: CN
Inventors: 喻敏; 黎宝怡; 何雨桐; 麦靖文; 李学文; 周彪; 张可国
Original assignee: Foshan University
Current assignee: Foshan University
Priority date: 2020-07-06
Filing date: 2020-07-06
Publication date: 2020-12-01

Abstract

The invention discloses a pea planting method, which comprises the following steps: (1) sterilizing and atomizing pea seeds; (2) culturing pea plants by adopting a water culture nutrient solution containing boron; (3) after culturing for 10-15 days, carrying out topping treatment on pea plants; (4) and (3) smearing strigolactones on each node part of the pea plants after topping. By using the pea planting method disclosed by the invention, the growth of peas can be promoted, the branching of peas can be inhibited, and the yield is increased. In the actual planting of peas, the yield of peas can be increased and accurate agriculture can be developed in a mode of combining high-effect fertilizer and strigolactone hormone.

Description

Method for promoting growth of peas and increasing yield of peas

[ technical field ] A method for producing a semiconductor device

The invention relates to the field of agricultural planting, in particular to a method for promoting pea growth and improving pea yield.

[ background of the invention ]

Pea is a plant belonging to genus Pisum of subfamily Papilionaceae of Leguminosae, namely Dutch bean, wheat bean, green bean, lima bean, Huai bean, golden bean, Bidou bean, and Han bean. Peas are the second largest edible legume crop after soybeans, are rich in protein, fat, carbohydrate, crude fiber, and various vitamins, and can be used as grains, vegetables, and feed. Because the growth period is short, the cold resistance is strong, the adaptability to soil is strong, and the food and vegetable are used together, the cultivation history has been more than 2000 years in China and the whole country is covered for a long time. On the other hand, the pea protein content is about 24 percent, which is higher than that of rice, wheat and corn, and is one of the important sources of human and livestock plant protein; the content of pea starch is about 46 percent, which is higher than that of small red beans, kidney beans, rice beans and pigeon peas, and is always the preferred raw material in the traditional vermicelli, cake and starch processing and manufacturing industries. With the increasing demand of market diversity, peas increasingly show important roles in the food processing industry, the pharmaceutical industry, the animal husbandry and other industries. In addition, the pea rhizobia can carry out biological nitrogen fixation, and 75-90 kg of nitrogen in the air can be fixed in each hectare of pea field, so that the method is favorable for improving the soil and promoting the yield increase of next-crop non-leguminous crops.

The growth potential of pea plants is strong, and each lateral bud can germinate to form a branch, a flower and a fruit. Particularly, after the plants are pinched, as more side branches usually germinate due to the transfer of the nutrition center, the germination and growth of the side branches consume a large amount of nutrient substances, and the yield of peas is reduced, the fruits are reduced, and the quality is poor. In addition, lateral branch clusters can cover each other, permeability is poor, and serious diseases and insect pests are easily caused. Therefore, in the growth and cultivation process of peas, measures such as pruning and branching are required to be carried out regularly to regulate the growth of plants, a great amount of manpower and time are consumed for the farm work operation, the production cost is obviously increased, and the plants are easy to cause germ infection when branching is carried out. Therefore, how to reduce labor intensity and production cost by reducing occurrence of pea side branches and reduce pathogen transmission becomes a great concern.

Although the growth and development of axillary buds are mainly controlled by genes, the surrounding environment (e.g., photoperiod, temperature, nutritional conditions) and plant hormones are also important factors affecting the development of axillary buds, which determine whether 1 axillary bud develops into a lateral branch or continues to sleep, for example, the deficiency of nutrients, especially phosphorus, inhibits branching and affects the position of axillary buds under a main branch. Since the discovery of 5 major plant hormones, auxin, abscisic acid, cytokinin, gibberellin, and ethylene, many studies have shown intrinsic factor regulation. Plant hormones regulate the morphology of plants by regulating cell division, differentiation, growth and death.

Strigolactones are a generic term for some natural strigol compounds and synthetic analogs. The reason why strigolactone was originally studied was that it could induce the germination of parasitic plant species. Researchers use high performance liquid chromatography tandem mass spectrometry (HPLC/MS/MS) to perform qualitative and quantitative analysis on the strigolactones in various plants, and further discover that the strigolactones or derivatives thereof have complex effects on the plants, and besides controlling overground branches, the strigolactones have a regulating mechanism and a synergistic effect with other hormones, and also relate to various growth, development and physiological and biochemical processes, including root growth, root hair growth, adventitious root fixation, secondary growth, photosynthetic morphogenesis, seed germination, growth of moss filaments and the like.

For a long time, the research on the pea high-yield planting technology in China is less, and the high-yield, high-quality, special and multi-resistant peas and the matched high-yield and high-efficiency cultivation technology are very lacking in production, so that the production level is generally low, and the yield potential is not fully excavated.

[ summary of the invention ]

The invention aims to provide a method for promoting pea growth and improving pea yield.

The main technical scheme of the invention is as follows:

a method of planting peas comprising the steps of:

(1) sterilizing and atomizing pea seeds;

(2) culturing pea plants by adopting a water culture nutrient solution containing boron;

(3) after culturing for 10-15 days, carrying out topping treatment on pea plants;

(4) and (3) smearing strigolactones on each node part of the pea plants after topping.

As a further improvement on the technical scheme, 7.5 percent of sodium hypochlorite is adopted to disinfect the seeds in the step (1), and CaCl with the concentration of 0.5mmol/L is used₂The solution is used for carrying out atomization culture on the seeds, and the atomization culture time is 48 h.

As a further improvement to the technical scheme, the concentration of boron in the water culture nutrient solution in the step (2) is 25 mu mol/L.

As a further improvement to the technical scheme, the water culture nutrient solution in the step (2) contains the following components:

Ca(NO₃)₂·4H₂O 2.5mmol/L；

MgSO₄·7H₂O 2mmol/L；

K₂SO₄ 2mmol/L；

KH₂PO₄ 1mmol/L；

KCl 0.05mmol/L；

FeSO₄·7H₂O 0.1mmol/L；

EDTA·Na₂ 0.1mmol/L；

ZnSO₄·7H₂O 4μmol/L；

MnCl₂ 2μmol/L；

(NH₄)₆Mo₇O₂₄·4H₂O 1μmol/L；

CuSO₄·5H₂O 0.5μmol/L。

as a further improvement to the above technical scheme, in the step (4), the concentration of the strigolactone is 1.5 mg/L.

By using the pea planting method disclosed by the invention, the growth of peas can be promoted, the branching of peas can be inhibited, and the yield is increased. In the actual planting of peas, the yield increase and the development of precision agriculture of peas can be promoted by means of high-effect fertilizers and combination of strigolactones.

[ description of the drawings ]

FIG. 1: the growth of pea axillary buds was treated with strigolactones at different concentrations without topping.

FIG. 2: the number of different concentrations of strigolactone treated axillary buds ranges without topping.

FIG. 3: strigolactone treated pea axillary bud lengths at different concentrations without topping.

FIG. 4: in different conditions, the growth of axillary buds of peas treated by strigolactones in different concentrations.

[ detailed description ] embodiments

The embodiments of the present invention will be described in more detail below with reference to the drawings and the reference numerals so that those skilled in the art can implement the embodiments after reading the description.

Example 1:

seed disinfection and aeroponics

Before pea cultivation, seeds need to be soaked in 7.5% sodium hypochlorite solution for disinfection for 30min,

then, the seeds are washed by sterile ultrapure water until the seeds have no smell of sodium hypochlorite solution, and the seeds are ensured to have no disinfectant residual. After the seeds are disinfected, CaCl with the concentration of 0.5mmol/L is used under the dark condition at the temperature of 24 DEG C₂Soaking the seeds in the solution for 8h, and performing atomization culture after the seeds absorb water and expand. Seeds were incubated at the same concentration of CaCl₂Culturing for 48h under the condition of solution atomization, and performing solution replacement once during the culture period to prevent decay.

Hydroponic culture

After the aeroponics, peas with the root length of about 3-4 cm are selected and transferred to a nutrient solution with the pH value of 5.5 for culture, and 25 mu mol/L of boron is added into the culture solution. The nutrient solution comprises the following specific formula:

table 1: the components and proportion of the nutrient solution

Remove the top

After the peas are transformed into water culture, the peas are subjected to topping treatment after 13 days according to the growth period of the plants.

Daubing strigolactone

Dipping a certain amount of 300 mu L of 0.5mg/L strigolactone solution respectively by using a medical cotton swab, wherein the daubing position of the strigolactone is the axillary bud position of each section of the pea plant, treating at the evening time every day, and continuously treating for 7 days.

Preparing strigolactone: weighing 1mg strigolactone (GR24), adding 5mL acetone for dissolving, sealing in a tinfoil paper centrifuge tube after the medicine is completely dissolved, placing in a refrigerator at 4 ℃ for later use, and diluting according to a proportion when in use.

Measuring and recording

After the completion, the pea plants were photographed to record the growth conditions, and the basic morphological indicators of the overground parts, the root systems and the like were measured. The measurement indexes of the overground part are plant height, internode distance and bud length. Plant height: the distance between the base of the plant and the top of the stem is indicated, and the distance between the height of the pea plant from the node of the cotyledon of the pea to the highest growing point of the main stem is measured in the experiment. Pitch spacing: the lengths between the node positions are measured by using a ruler.

Measurement of shoot length: in order to conveniently observe and record the growth condition of each axillary bud, the axillary bud growing at each node position is cut from the stem, the buds growing or not growing at each node leaf axillary position are placed on an EPSON EXPRESSION 11000XL scanner to be scanned into a picture according to the node position sequence, and then the length measurement is carried out by using image processing software ImageJ.

Example 2:

example 2 was conducted in substantially the same manner as example 1 except that the strigolactone concentration was 1.5 mg/L.

Comparative examples 1 to 6:

comparative example 1 and example 1 were performed essentially the same except that the plants were not topped, the hydroponic nutrient solution contained no boron, and the plants were not coated with strigolactone.

Comparative example 2 and example 1 were performed in essentially the same manner except that the plants were not topped, the hydroponic nutrient solution contained no boron and the strigolactone concentration was 0.5 mg/L.

Comparative example 3 and example 1 were performed in essentially the same manner except that the plants were not topped, the hydroponic nutrient solution contained no boron and the strigolactone concentration was 1.5 mg/L.

Comparative example 4 and example 1 were performed essentially the same except that the plants were not topped.

Comparative example 5 and example 1 were conducted in substantially the same manner except that the plants were not topping treated and the concentration of strigolactone was 0.5 mg/L.

Comparative example 6 and example 1 were conducted in substantially the same manner except that the plants were not topping treated and the concentration of strigolactone was 1.5 mg/L.

As shown in FIG. 1, the results of comparative examples 1-6 are shown, and the axillary buds of each node of the plant are arranged according to the order of the node, so that the phenotype condition of the axillary buds of the plant can be obviously seen. In fig. 1: (b) and (c) respectively carrying out exogenous strigolactone treatment after 13d of nutrient solution culture of boron deficiency (B0), wherein the concentrations are 0.5mg/L and 1.5mg/L respectively, and continuously treating the growth condition of each section of axillary buds of peas after 7 d; (d) pea growth of each axillary bud after 20 days of nutrient solution culture containing 25 μmol/L (B25) boric acid, as a control; (e) and (f) respectively carrying out exogenous strigolactone treatment after 13d of nutrient solution culture containing 25 mu mol/L (B25) boric acid, wherein the concentrations are 0.5mg/L and 1.5mg/L respectively, and continuously treating for 7d to obtain the growth condition of each section of axillary bud of pea; wherein, the 0 section is the implantation point of the pea cotyledon, the 1 section is the 1 st section of the pea section, and so on. The axillary buds of the peas are sequentially placed upwards according to the node order. The length measurement of the buds is counted by the longest bud in the two buds at the same node.

According to FIG. 1, under the condition of not removing the top, and under the condition of lacking boron (B0), the apical dominance of the plant is relieved, the axillary buds are developed, and the inhibitory effect of the exogenous SL on the axillary buds is not obvious; under the condition of adding boron (B25), exogenous SL is applied at the same time, the apical dominance of the plant is obvious, the growth vigor is good, the longitudinal growth is presented, the axillary buds of each node are smaller, and the axillary buds do not show the extension and development. The synergistic effect exists between boron and strigolactone, and plant branching is regulated and controlled together.

FIG. 2 shows the results of comparative examples 1 to 6. When the axillary buds of the pea plants are classified and counted, the axillary buds with the length less than 0.1cm are removed, and the lengths of the other axillary buds are counted according to the range. Wherein 0.1-0.5cm is undeveloped axillary bud, 0.5-2cm is developed axillary bud, and more than 2cm is rapidly elongated axillary bud. Under the condition of B0, the axillary buds of the Control plant (Control) which is not subjected to strigolactone treatment develop greatly, wherein the number of the axillary buds is the most at 0.5-2cm, and the average number of the individual plants is 5.0; and has rapidly elongated axillary buds (greater than 2cm) with an average of 2.875 individuals. After exogenous strigolactone is applied, the distribution change of the plant bud length is obvious, and the bud length is concentrated in the ranges of 0.1-0.5cm and 0.5-2 cm. When the strigolactone concentration is 0.5mg/L, the average number of the single plant buds is 5.375 and 6.125 respectively; when the addition concentration of the exogenous strigolactone is 1.5mg/L, the range of the bud length is more concentrated on 0.1-0.5cm, the average single plant number reaches 7.0, and the number in other ranges is less or none. The axillary bud growth is shortened along with the increase of the strigolactone concentration, and the exogenous application of 1.5mg/L strigolactone completely inhibits the growth of the axillary bud of the plant. The axillary buds of the pea plants cultured under the condition of adding boron (B25) are smaller, the number of the axillary buds is intensively distributed in the range of 0.1-0.5cm, compared with the axillary buds without daubing strigolactones, the number of the axillary buds with the daubing strigolactones shows a decreasing trend along with the increase of the concentration, and the strigolactones inhibit the growth of the axillary buds of the pea plants.

In fig. 2, note: b0 shows that peas were cultured in nutrient solution in boron-deficient environment (0. mu. mol/L) for 20 days, B25 shows that peas were cultured in nutrient solution containing 25. mu. mol/L boric acid concentration for 20 days; counting the number of buds according to the length range, wherein the ranges are respectively 0.1-0.5cm, 0.5-2cm and more than 2 cm; wherein shoot lengths less than 0.1cm are considered as non-developing axillary buds and are not included in the statistics of the number. Control: performing control, performing no treatment when culturing for 13d, continuing for 7d, and performing co-culture for 20 d; SL 0.5, when the plant is cultured for 13 days, exogenous application concentration is 0.5mg/L strigolactone; SL 1.5: when the plants are cultured for 13 days, 1.5mg/L strigolactone is exogenously applied.

FIG. 3 shows the results of comparative examples 1 to 6. When the pea grows to day 13, the pea plants with different boron concentrations (B0, B25) are treated with strigolactones with different concentrations, the growth of the axillary buds of the plants is observed and the length of the axillary buds of each section of the pea plants is measured and analyzed. As shown in FIG. 3a, under the condition of boron deficiency (B0), the length of each axillary bud of the plants treated by applying exogenous strigolactone with different concentrations is smaller than that of the axillary bud of the Control (Control) by taking the plants not treated by exogenous strigolactone as the Control. The axillary buds of the plant at the 0 th to 3 th nodes have obvious length change after exogenous strigolactones are applied, the strigolactones have inhibition effect on the growth of the axillary buds, and the nodes after the 4 th node have no obvious difference. Wherein the inhibition effect of the concentration of 1.5mg/L is more obvious than that of 0.5mg/L, and the inhibition effect of exogenous strigolactone on the growth of axillary buds is enhanced along with the increase of the strigolactone treatment concentration; the strigolactone treatment with different concentrations has the most obvious inhibition effect on the axillary buds at the second section of the pea plant, and the strigolactone treatment reaches the level of obvious difference. This indicates that the effect of strigolactone on plants requires lower doses to achieve more significant inhibitory effect. Under the condition of containing boron (B25) (figure 3.3B), after the treatment of exogenous strigolactones with different concentrations, the length of the axillary bud at each node of the plant is not obviously different from that of the control, and the length of the axillary bud is kept at about 0.2cm on average.

In fig. 3, note: b0 shows that peas were cultured in nutrient solution in boron-deficient environment (0. mu. mol/L) for 20 days, B25 shows that peas were cultured in nutrient solution containing 25. mu. mol/L boric acid concentration for 20 days; (a) and (B) are axillary bud length analysis histograms counted after culturing the axillary buds in nutrient solutions of B0 and B25 for 13 days and processing the axillary buds with strigolactones at different concentrations after continuously processing for 7 days. Control is used as a Control, no treatment is carried out at 13d, and the culture is continued to 20 d; SL 0.5 is exogenously applied with strigolactone treatment, and the concentration is 0.5 mg/L; SL1.5 is treated by applying strigolactone from an external source, and the concentration is 1.5 mg/L. Wherein, the 0 section is the implantation point of the pea cotyledon, the 1 section is the 1 st section of the pea section, and so on. The axillary buds of the peas are sequentially placed upwards according to the node order. The length measurement of the buds is counted by the longest bud in the two buds at the same node.

The above results show that the boron deficiency can promote the growth of plant axillary buds, and meanwhile, after the exogenous strigolactone is applied on the basis, the axillary buds are obviously inhibited, the bud length is shortened along with the increase of the strigolactone concentration, the inhibition effect of the applied exogenous strigolactone on the plant axillary buds is strengthened, the dosage effect is shown, the growth of the axillary buds at the 0-3 nodes of the plant is inhibited, the inhibition effect on the 2 nd and the 3 rd nodes is obvious, wherein 0.5mg/L already shows the obvious inhibition effect, and 1.5mg/L completely inhibits the development of the axillary buds. Under the condition of adding boron, the inhibition effect on the axillary buds of the peas is maintained, and the number and the length of the buds are reduced; the results reflect that the interaction of boron and strigolactone jointly influences the growth and development of pea axillary buds.

Comparative examples 7 to 13:

comparative example 7 and example 1 were performed essentially the same except that the plants were not topped, the hydroponic nutrient solution contained no boron, and the plants were not coated with strigolactone.

Comparative example 8 and example 1 were performed in substantially the same manner except that the hydroponic nutrient solution contained no boron and the plants were not smeared with strigolactone.

Comparative example 9 and example 1 were performed essentially the same except that the plants were not topped and the hydroponic nutrient solution contained no boron.

Comparative example 10 and example 1 were run essentially the same except that the hydroponic nutrient solution contained no boron.

Comparative example 11 and example 1 were conducted in substantially the same manner except that the plants were not topping treated and were not smeared with strigolactone.

Comparative example 12 and example 1 were performed essentially the same except that the plants were not coated with strigolactone.

Comparative example 13 and example 1 were performed essentially the same except that the plants were not topped.

FIG. 4 shows the results of comparative examples 7 to 13 and example 1. Under the condition of boron deficiency (B0), compared with a plant without removing the top (figure 4a), the axillary buds of the plant after the top removing treatment (figure 4c) and 7d are not obviously changed, all the axillary buds are developed, particularly, the 0-3 nodes are obvious, and the axillary buds without removing the top and the top are not obviously different, thereby proving that the apical dominance of the pea plant is relieved due to the boron deficiency again. Under the condition of adding boron (B25), compared with the condition of not removing the top (figure 4B), the pea plant which is removed with the boron (figure 4d) grows well and axillary buds of each node grow vigorously, and the axillary buds of higher nodes also grow: after the apical removal treatment (QD), the axillary buds of peas under the conditions of B0 and B25 are developed, but the difference is that the axillary buds are relatively long and concentrated on 0-3 nodes in the absence of boron, and the development of 3-6 nodes on the upper part of a plant is more obvious when boron is added; this is related to the loss of apical dominance of the plant after topping, the significant decrease of the inhibition of the plant by apical bud auxin, and the vigorous growth of the plant and the rapid development of the axillary buds under the condition of sufficient supply of nutritional conditions.

Under the condition of boron deficiency (B0) (figures 4a, c, e and g), taking a plant which is not treated by other treatments as a control (figure 4a), the axillary buds of each node of the plant which is subjected to independent topping (QD, figure 4c) are longer than the control, wherein the length of the 3 rd node is obviously increased, and the growth of the axillary buds of the plant is promoted under the condition of no inhibition of the apical bud auxin; after strigolactone was added to the plants, the axillary buds of the plants were inhibited again to some extent compared to the Control (B0Control, FIG. 4a) (FIG. 4 e). This indicates that exogenous strigolactone has inhibitory effect on the development of axillary buds of pea plants.

Additionally, when strigolactone was exogenously applied to the plants (fig. 4e, f), the following was found after analysis: under the condition of adding boron (B25), the axillary buds of the peas have no obvious difference with the control after the strigolactones are applied, and under the action of the boron, the plants still inhibit the growth of the axillary buds under the common regulation and control of the apical bud auxin and the strigolactones; meanwhile, by taking a plant without topping as a control (fig. 4f), topping treatment is carried out simultaneously on the basis (fig. 4h), the apical dominance of the plant is relieved, the plant loses the inhibition effect of apical bud auxin on axillary buds, and each section of axillary buds of the plant are developed obviously, wherein the 2 nd and 3 rd sections are most obvious, but the axillary buds are not completely recovered to the level of topping alone compared with topping treatment (QD) alone (fig. 4 d).

In the treatment of different boron concentrations (B0 and B25), the growth and development differences of pea axillary buds are mainly concentrated on 0-3 nodes, and when the analysis and comparison of the bud length of the 0-3 nodes are carried out, the responses of different nodes to the apical bud auxin and the strigolactone are also different under the same boron concentration treatment condition. The expression is as follows: during the topping treatment (QD), boron (B25) is added to the plant, so that 2 nd and 3 rd axillary buds are obviously developed, and 0-3 rd nodes of boron (B0) are developed; when the strigolactone is coated, the length of the axillary bud is recovered to a control level, compared with different boron concentrations, the length of the 1-2 section bud keeps a different level, and the inhibition effect of the strigolactone on the 3 rd section position is reduced in the absence of boron, and the difference is not obvious from B25. When the strigolactone is applied at the same time and the top is removed, the length of 0-3 section buds is not obviously different from that of the single top removal (QD), but the level can not be completely recovered, and the inhibition effect on the 3 section buds is obvious under the condition of adding boron.

In fig. 4, note: b0 shows that peas were cultured in nutrient solution in boron-deficient environment (0. mu. mol/L) for 20 days, B25 shows that peas were cultured in nutrient solution containing 25. mu. mol/L boric acid concentration for 20 days; (a) and (B) axillary buds of each node counted after 7d of treatment were continued without treatment after culturing in nutrient solutions of B0 and B25 for 13d, respectively. (c) And (d) respectively carrying out topping treatment when the plants are cultured in B0 and B25 for 13d, and continuously treating axillary buds after 7 d; (e) and (f) axillary buds obtained after culturing in B0 and B25 for 13 days and applying 0.5mg/L of strigolactone SL to the axillary buds after 7 days; (g) and (h) axillary buds after 7 days were treated by culturing in B0 and B25 for 13 days, respectively, and then applying 0.5mg/L of strigolactone while removing the apical bud.

The exogenous strigolactone is applied for treatment after topping, the axillary buds of the pea plants after topping are better developed, the axillary buds of the pea plants under the conditions of B0 and B25 are developed, and the difference is that the axillary buds are larger in length and are concentrated on 0-3 nodes when boron is absent, and the development condition of the 3-6 nodes on the upper parts of the plants is more obvious when boron is added; the strigolactone has a regulation effect on the plant type of the pea plant, participates in the regulation of the plant phenotype together with the terminal bud auxin, and boron is also involved in the regulation as an important nutrient element, wherein the strigolactone can inhibit the pea axillary bud by cooperating with the terminal bud auxin, and can also indirectly inhibit the growth of the pea axillary bud under the action of the boron alone.

The foregoing is illustrative of the preferred embodiment of the present invention and is not to be construed as limiting thereof in any way, and therefore, all modifications and variations that fall within the spirit of the invention are intended to be included within the scope thereof.

Claims

1. A method of planting peas comprising the steps of:

(1) sterilizing and atomizing pea seeds;

2. The method of growing peas in claim 1 wherein in step (1) the seeds are sterilized with 7.5% sodium hypochlorite with 0.5mmol/L CaCl₂The solution is used for carrying out atomization culture on the seeds, and the atomization culture time is 48 h.

3. The method of growing peas in claim 1, wherein the concentration of boron in the water culture nutrient solution in step (2) is 25 μmol/L.

4. The method of growing peas in claim 1 wherein the water culture nutrient solution in step (2) contains the following components:

Ca(NO₃)₂·4H₂O2.5 mmol/L；

MgSO₄·7H₂O2 mmol/L；

K₂SO₄2 mmol/L；

KH₂PO₄ 1mmol/L；

KCl0.05 mmol/L；

FeSO₄·7H₂O 0.1mmol/L；

EDTA·Na₂ 0.1mmol/L；

ZnSO₄·7H₂O 4μmol/L；

MnCl₂2μmol/L；

(NH₄)₆Mo₇O₂₄·4H₂O1μmol/L；

CuSO₄·5H₂O0.5μmol/L。

5. the method of growing peas according to claim 1, wherein the concentration of strigolactones in step (4) is 1.5 mg/L.