CN104829875A - Polysaccharide mixture with skin whitening effect and method for manufacturing polysaccharide mixture - Google Patents
Polysaccharide mixture with skin whitening effect and method for manufacturing polysaccharide mixture Download PDFInfo
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Abstract
The invention relates to the technical field of cosmetics, in particular to a polysaccharide mixture with a skin whitening effect and a method for manufacturing the polysaccharide mixture. The polysaccharide mixture with the skin whitening effect comprises, by weight, 40-50 parts of radix paeoniae alba polysaccharides, 20-25 parts of angelica sinensis polysaccharides and 20-25 parts of angelica dahurica polysaccharides. The radix paeoniae alba polysaccharides, the angelica sinensis polysaccharides and the angelica dahurica polysaccharides are mixed with one another to obtain the polysaccharide mixture. The polysaccharide mixture and the method have the advantages that ethanol precipitation conditions are controlled, so that ineffective polysaccharide ingredients in the total polysaccharides of radix paeoniae alba, angelica sinensis and angelica dahurica can be abandoned, polysaccharide active portions, which are capable of obviously suppressing tyrosinase, suppressing generation of melanin and realizing the excellent skin whitening effect, in the total polysaccharides can be screened, the purity of functional polysaccharides of the active portions can be greatly improved, the purity of active polysaccharide ingredients with skin whitening and anti-oxidation effects can be improved, and the activity of the polysaccharide mixture can be enhanced; active polysaccharide components extracted from three traditional Chinese medicinal materials which are the radix paeoniae alba, the angelica sinensis and the angelica dahurica are mixed with one another according to certain proportions and are coordinated with one another, so that the excellent effects can be realized.
Description
Technical field
The present invention relates to cosmetic technical field, be specifically related to a kind of polysaccharide mixture with whitening function and preparation method thereof.
Background technology
Tyrosine oxidase is the main restriction enzyme in melanin biosynthesis approach, and current whitening class makeup reduce melanic formation in skin mainly through restraint of tyrosinase.Research whitening function, generally selects Tyrosinase inhibition assays generally to be accepted by numerous scholars as evaluation whitening effect.On market, whitening class makeup are of a great variety, but mostly containing chemical composition, may there are some disadvantageous effects to skin, even have carcinogenesis.The tyrosine kinase inhibitors in natural drug source, green, safety, not only effective for pigment type tetter, brightening, cosmetics formula is significant.
" root of herbaceous peony separation of polysaccharides Purification and properties is analyzed. biology magazine [J], 2008,25 (5): 34-36 " in disclose separation and purification in root of herbaceous peony water extraction solution and obtain a kind of polysaccharide protein complex, and prove that it is homogeneous polysaccharide component;
" technical study of microwave extraction Radix Angelicae Sinensis whitening composition. daily chemical industry [J], 2004,34 (5): 335-337 " in disclose microwave extraction Radix Angelicae Sinensis whitening composition, its whitening index is extract;
" experimental study of root of Dahurain angelica whitening lotion vitro inhibition tyrosinase activity. Changchun University of Traditional Chinese Medicine's journal [J]; 2012; 28 (6): 960-961 " in disclose the experimental study of root of Dahurain angelica whitening lotion vitro inhibition tyrosinase activity, mentioning effective constituent in the preparation method of root of Dahurain angelica whitening lotion in literary composition is volatile oil;
From upper description, to the root of herbaceous peony in prior art, Radix Angelicae Sinensis and the root of Dahurain angelica also have research, but mainly concentrate on the utilization of whole medicine, concentrate on protective foods, the fields such as medicine and extraction process, also some herbal polysaccharide extracts of bibliographical information or its composition is had to have whitening function etc., but these herbal polysaccharide molecular weight ranges are larger, it is a mixture, can be described as the total polysaccharides of certain Chinese medicine, the composition that wherein can play whitening function or free radical scavenging function is certain part in total polysaccharides, other amount of activated too low or substantially without effect, the total polysaccharides of such Chinese medicine is owing to causing whitening function or free radical scavenging effect greatly to reduce containing the activeconstituents worked and adiaphorous non-active ingredient simultaneously, play active polysaccharide component purity also just greatly to reduce.
Summary of the invention
Technical problem to be solved by this invention is: provide good polysaccharide mixture of a kind of whitening function effect and preparation method thereof.
In order to solve the problems of the technologies described above, the technical solution used in the present invention is: provide a kind of polysaccharide mixture with whitening function, the raw material comprising following weight part mixes: root of herbaceous peony polysaccharide 40-50 part; Radix Angelicae Sinensis polysaccharide 20-25 part; Angelica dahurica polysaccharide 20-25 part.
Another technical scheme of the present invention, for providing a kind of preparation method with the polysaccharide mixture of whitening function, comprises the steps: polysaccharide mixture root of herbaceous peony polysaccharide, Radix Angelicae Sinensis polysaccharide, angelica dahurica polysaccharide must after mixing by the part by weight of 40-50:20-25:20-25 with whitening function.
Beneficial effect of the present invention is: the present invention is from the root of herbaceous peony, Radix Angelicae Sinensis, in root of Dahurain angelica total polysaccharides, by controlling alcohol precipitation condition, discard invalid polysaccharide component, filtering out in total polysaccharides can remarkable restraint of tyrosinase, the production of check melanin, the good active polysaccharide part of whitening function, the active part purity of polysaccharide worked can be made greatly to improve, make that there is whitening, the active polysaccharide material of antioxygenation improves, and then increased activity, and the above-mentioned active polysaccharide ingredient extracted from three kinds of Chinese medicinal materialss is mixed by a certain percentage, serve mutual coordination, the effect of reinforced effects.
Embodiment
By describing technology contents of the present invention in detail, being realized object and effect, be explained below in conjunction with embodiment.
The design of most critical of the present invention is: from the root of herbaceous peony, Radix Angelicae Sinensis, root of Dahurain angelica total polysaccharides, by controlling alcohol precipitation condition, discard invalid polysaccharide component, filter out in total polysaccharides the active polysaccharide part playing and there is whitening function or free radical scavenging effect, and mix by a certain percentage, serve mutual coordination, the effect that effect is better.
The invention provides a kind of polysaccharide mixture with whitening function, the raw material comprising following weight part mixes: root of herbaceous peony polysaccharide 40-50 part; Radix Angelicae Sinensis polysaccharide 20-25 part; Angelica dahurica polysaccharide 20-25 part.
The above-mentioned preparation method with the polysaccharide mixture of whitening function comprises the steps: root of herbaceous peony polysaccharide, Radix Angelicae Sinensis polysaccharide, angelica dahurica polysaccharide to mix by above-mentioned part by weight, must have the polysaccharide mixture of whitening function after grinding evenly.
Above-mentioned have in the preparation method of the polysaccharide mixture of whitening function, and the preparation method of described root of herbaceous peony polysaccharide comprises:
Get root of herbaceous peony root to pulverize, add soaked in absolute ethyl alcohol 24 hours, volume and the root of herbaceous peony root weight ratio of described dehydrated alcohol are 8:1; Filtration discards ethanol, and the gained dregs of a decoction continue to add soaked in absolute ethyl alcohol 24 hours, and volume and the root of herbaceous peony root weight ratio of described dehydrated alcohol are 6:1; Filtration discards ethanol, volatilizes ethanol after obtaining the dregs of a decoction;
The distilled water 95 DEG C again dregs of a decoction being added 10 times of weight ratios extracts 2 hours, and double gauze filters, and retains filtrate; The dregs of a decoction continue the distilled water adding 8 times of weight ratios, and 95 DEG C are extracted 2 hours, and double gauze filters, and retain filtrate; Merge twice gauze and filter rear gained filtrate, make filtrate in boiling water bath, be concentrated into 1/3rd of volume;
Concentrated rear filtrate is placed in 4 DEG C of ice-water baths, after solution temperature is stable, slowly adding dehydrated alcohol makes alcohol content reach 35-45% (V/V), limit edged stirs, be placed in 4 DEG C of frozen water after 12 hours, centrifugal removing precipitation, retain supernatant liquor, in supernatant liquor, add dehydrated alcohol afterwards again makes alcohol content reach 55-65% (V/V), limit edged stirs, and is placed in 4 DEG C of frozen water and obtains solution A after 12 hours, and removing solution A supernatant liquor obtains solution B, solution B uses centrifuge 20 minutes described centrifuge speeds to be 4000r/min, obtains the polysaccharide precipitation of solution B;
Described polysaccharide precipitation such as to use respectively at weight acetone, the washed with diethylether, centrifugal removing acetone and ether solvent after washing, gained precipitation is dissolved in the distilled water of its 2 times of weight ratios, be placed in dialysis tubing dialyse 24 hours, the molecular weight cut-off of described dialysis tubing is less than 6000, and gained retains the powder of liquid after lyophilize and is root of herbaceous peony polysaccharide.
The preparation method of described Radix Angelicae Sinensis polysaccharide comprises:
Get angelica root to pulverize, add soaked in absolute ethyl alcohol 24 hours, volume and the root of herbaceous peony root weight ratio of described dehydrated alcohol are 8:1; Filtration discards ethanol, and the gained dregs of a decoction continue to add soaked in absolute ethyl alcohol 24 hours, and volume and the root of herbaceous peony root weight ratio of described dehydrated alcohol are 6:1; Filtration discards ethanol, volatilizes ethanol after obtaining the dregs of a decoction;
The distilled water 95 DEG C again dregs of a decoction being added 10 times of weight ratios extracts 2 hours, and double gauze filters, and retains filtrate; The dregs of a decoction continue the distilled water adding 8 times of weight ratios, and 95 DEG C are extracted 2 hours, and double gauze filters, and retain filtrate; Merge twice gauze and filter rear gained filtrate, make filtrate in boiling water bath, be concentrated into 1/3rd of volume;
Concentrated rear filtrate is placed in 4 DEG C of ice-water baths, after solution temperature is stable, slowly adding dehydrated alcohol makes alcohol content reach 35-45% (V/V), limit edged stirs, be placed in 4 DEG C of frozen water after 12 hours, centrifugal removing precipitation, retain supernatant liquor, in supernatant liquor, add dehydrated alcohol afterwards again makes alcohol content reach 75-85% (V/V), limit edged stirs, and is placed in 4 DEG C of frozen water and obtains solution A after 12 hours, and removing solution A supernatant liquor obtains solution B, solution B uses centrifuge 20 minutes described centrifuge speeds to be 4000r/min, obtains the polysaccharide precipitation of solution B;
Described polysaccharide precipitation such as to use respectively at weight acetone, the washed with diethylether, centrifugal removing acetone and ether solvent after washing, gained precipitation is dissolved in the distilled water of its 2 times of weight ratios, be placed in dialysis tubing dialyse 24 hours, the molecular weight cut-off of described dialysis tubing is less than 6000, and gained retains the powder of liquid after lyophilize and is Radix Angelicae Sinensis polysaccharide.
The preparation method of described angelica dahurica polysaccharide comprises:
Get root of Dahurain angelica root to pulverize, add soaked in absolute ethyl alcohol 24 hours, volume and the root of herbaceous peony root weight ratio of described dehydrated alcohol are 8:1; Filtration discards ethanol, and the gained dregs of a decoction continue to add soaked in absolute ethyl alcohol 24 hours, and volume and the root of herbaceous peony root weight ratio of described dehydrated alcohol are 6:1; Filtration discards ethanol, volatilizes ethanol after obtaining the dregs of a decoction;
The distilled water 95 DEG C again dregs of a decoction being added 10 times of weight ratios extracts 2 hours, and double gauze filters, and retains filtrate; The dregs of a decoction continue the distilled water adding 8 times of weight ratios, and 95 DEG C are extracted 2 hours, and double gauze filters, and retain filtrate; Merge twice gauze and filter rear gained filtrate, make filtrate in boiling water bath, be concentrated into 1/3rd of volume;
Concentrated rear filtrate is placed in 4 DEG C of ice-water baths, after solution temperature is stable, slowly adding dehydrated alcohol makes alcohol content reach 45-55%, limit edged stirs, be placed in 4 DEG C of frozen water after 12 hours, centrifugal removing precipitation, retain supernatant liquor, in supernatant liquor, add dehydrated alcohol afterwards again makes alcohol content reach 80-90%, be placed in 4 DEG C of frozen water and obtain solution A after 12 hours, removing solution A supernatant liquor obtains solution B, and solution B uses centrifuge 20 minutes described centrifuge speeds to be 4000r/min, obtains the polysaccharide precipitation of solution B;
Described polysaccharide precipitation such as to use respectively at weight acetone, the washed with diethylether, centrifugal removing acetone and ether solvent after washing, gained precipitation is dissolved in the distilled water of its 2 times of weight ratios, be placed in dialysis tubing dialyse 24 hours, the molecular weight cut-off of described dialysis tubing is less than 6000, and gained retains the powder of liquid after lyophilize and is angelica dahurica polysaccharide.
Embodiment 1
The invention provides a kind of preparation method with the polysaccharide mixture of whitening function, comprise the steps:
Root of herbaceous peony polysaccharide preparation flow: get root of herbaceous peony dry root, pulverizes, and shines for standard to cross 80 orders, add dehydrated alcohol 8 times amount (V/W), soak 24 hours, filter, discard ethanol, the dregs of a decoction continue to add dehydrated alcohol 6 times amount (V/W), soak 24 hours, filter, discard ethanol, obtain the dregs of a decoction, under putting sunlight, naturally ventilated drying, volatilizes (volatile dry) ethanol.Dregs of a decoction transfer is put in beaker, add 10 times amount distilled water, 95 DEG C are extracted 2 hours, double gauze filters, retain filtrate, the dregs of a decoction continue to add 8 times amount distilled water, 95 DEG C are extracted 2 hours, double gauze filters, merging filtrate is in Erlenmeyer flask, 1/3rd of filtrate volume are concentrated in boiling water bath, Erlenmeyer flask is placed in 4 DEG C of ice-water baths, liquid temp to be filtered is stablized, slowly adding dehydrated alcohol makes alcohol content reach 35-45%, limit edged stirs, to put in frozen water 12 hours, centrifugal removing precipitation, retain this supernatant liquor, continuing to add dehydrated alcohol makes alcohol content reach 55-65%, limit edged stirs, to put in frozen water 12 hours, careful removing supernatant liquor, lower floor uses centrifuge 20 minutes (4000r/min), retain polysaccharide precipitation, use equimultiple acetone, after washed with diethylether, centrifugal except desolventizing, precipitation is dissolved in 2 times amount distilled water, be placed in dialysis tubing (molecular weight cut-off < 6000) dialysis 24 hours, namely root of herbaceous peony polysaccharide powder is obtained through lyophilize.
Radix Angelicae Sinensis polysaccharide preparation flow: get Radix Angelicae Sinensis dry root, pulverizes, and shines for standard to cross 80 orders, add dehydrated alcohol 8 times amount (V/W), soak 24 hours, filter, discard ethanol, the dregs of a decoction continue to add dehydrated alcohol 6 times amount (V/W), soak 24 hours, filter, discard ethanol, obtain the dregs of a decoction, under putting sunlight, naturally ventilated drying, volatilizes ethanol.Dregs of a decoction transfer is put in beaker, add 10 times amount distilled water, 95 DEG C are extracted 2 hours, double gauze filters, retain filtrate, the dregs of a decoction continue to add 8 times amount distilled water, 95 DEG C are extracted 2 hours, double gauze filters, merging filtrate is in Erlenmeyer flask, 1/3rd of filtrate volume are concentrated in boiling water bath, Erlenmeyer flask is placed in 4 DEG C of ice-water baths, liquid temp to be filtered is stablized, slowly adding dehydrated alcohol makes alcohol content reach 35-45%, limit edged stirs, to put in frozen water 12 hours, centrifugal removing precipitation, retain this supernatant liquor, continuing to add dehydrated alcohol makes alcohol content reach 75-85%, to put in frozen water 12 hours, careful removing supernatant liquor, lower floor uses centrifuge 20 minutes (4000r/min), retain polysaccharide precipitation, use equimultiple acetone, after washed with diethylether, centrifugal except desolventizing, unique precipitation is dissolved in 2 times amount distilled water, be placed in dialysis tubing (molecular weight cut-off < 6000) dialysis 24 hours, namely Radix Angelicae Sinensis polysaccharide powder is obtained through lyophilize.
Angelica dahurica polysaccharide preparation flow: get root of Dahurain angelica dry root, pulverizes, and shines for standard to cross 80 orders, add dehydrated alcohol 8 times amount (V/W), soak 24 hours, filter, discard ethanol, the dregs of a decoction continue to add dehydrated alcohol 6 times amount (V/W), soak 24 hours, filter, discard ethanol, obtain the dregs of a decoction, under putting sunlight, naturally ventilated drying, volatilizes ethanol.Dregs of a decoction transfer is put in beaker, add 10 times amount distilled water, 95 DEG C are extracted 2 hours, double gauze filters, retain filtrate, the dregs of a decoction continue to add 8 times amount distilled water, 95 DEG C are extracted 2 hours, double gauze filters, merging filtrate is in Erlenmeyer flask, 1/3rd of filtrate volume are concentrated in boiling water bath, Erlenmeyer flask is placed in 4 DEG C of ice-water baths, liquid temp to be filtered is stablized, slowly adding dehydrated alcohol makes alcohol content reach 45-55%, limit edged stirs, to put in frozen water 12 hours, centrifugal removing precipitation, retain this supernatant liquor, continuing to add dehydrated alcohol makes alcohol content reach 80-90%, limit edged stirs, to put in frozen water 12 hours, careful removing supernatant liquor, lower floor uses centrifuge 20 minutes (4000r/min), retain polysaccharide precipitation, use equimultiple acetone, after washed with diethylether, centrifugal except desolventizing, unique precipitation is dissolved in 2 times amount distilled water, be placed in dialysis tubing (molecular weight cut-off < 6000) dialysis 24 hours, namely angelica dahurica polysaccharide powder is obtained through lyophilize.
The root of herbaceous peony polysaccharide of above-mentioned preparation, Radix Angelicae Sinensis polysaccharide, angelica dahurica polysaccharide are mixed by 50%:25%:25% mass ratio, grinding evenly, must have the polysaccharide mixture of whitening function.
Embodiment 2
Get the root of herbaceous peony, Radix Angelicae Sinensis, root of Dahurain angelica 50g respectively, 50g, 50g, be placed in 3 Erlenmeyer flasks respectively, add dehydrated alcohol 10 times amount, soak 24h, double gauze was considered, and discarded ethanol, and the dregs of a decoction continue to add dehydrated alcohol 8 times amount, soak 24h, double gauze was considered, and discarded ethanol, and the dregs of a decoction are placed in vacuum drying oven, 60 DEG C of drying under reduced pressure 5h, obtain the root of herbaceous peony, Radix Angelicae Sinensis, the root of Dahurain angelica dregs of a decoction respectively.Respectively with the distilled water 95 DEG C of 10,8 times amount (500ml, 400ml), extract 4h, in 80 DEG C of water-baths, be concentrated into 250ml.
Alcohol precipitation condition: root of herbaceous peony Aqueous extracts: add dehydrated alcohol slowly and make alcohol content reach 35-45% (V/V) in root of herbaceous peony concentrated solution, limit edged stirs, to put in frozen water 12 hours, 20 minutes (4000r/min) centrifugal treating, discard precipitation, retain this supernatant liquor, continue to add dehydrated alcohol and make alcohol content reach 55-65%; Radix Angelicae Sinensis Aqueous extracts: slowly add dehydrated alcohol make alcohol content reach 35-45% (V/V) to Radix Angelicae Sinensis is concentrated, limit edged stirs, to put in frozen water 12 hours, 20 minutes (4000r/min) centrifugal treating, discard precipitation, retain this supernatant liquor, continue to add dehydrated alcohol and make alcohol content reach 75-85%; Root of Dahurain angelica Aqueous extracts: slowly add dehydrated alcohol and make alcohol content reach 45-55% in root of Dahurain angelica concentrated solution, limit edged stirs, and to put in frozen water 12 hours, and centrifugal removing precipitates, and retains this supernatant liquor, continues to add dehydrated alcohol and make alcohol content reach 80-90%.Respectively three kinds of solution to be put in frozen water 12 hours, careful removing supernatant liquor, lower floor uses centrifuge 20 minutes (4000r/min), retain polysaccharide precipitation, after using equimultiple acetone, washed with diethylether, centrifugal except desolventizing, precipitation is dissolved in 2 times amount distilled water, be placed in dialysis tubing (molecular weight cut-off < 6000) dialysis 24 hours, namely obtain root of herbaceous peony polysaccharide, Radix Angelicae Sinensis polysaccharide, angelica dahurica polysaccharide powder each about 1.15g, 0.92g, 0.45g through lyophilize.Get root of herbaceous peony polysaccharide, Radix Angelicae Sinensis polysaccharide, angelica dahurica polysaccharide powder 0.5g, 0.1g, 0.1g ground and mixed respectively evenly.
Embodiment 3
The extraction with the polysaccharide mixture of whitening function of the present invention:
Get the root of herbaceous peony, Radix Angelicae Sinensis, root of Dahurain angelica 100g respectively, 100g, 100g, be placed in 3 Erlenmeyer flasks respectively, add dehydrated alcohol 10 times amount, soak 24h, double gauze was considered, and discarded ethanol, and the dregs of a decoction continue to add dehydrated alcohol 8 times amount, soak 24h, double gauze was considered, and discarded ethanol, and the dregs of a decoction are placed in vacuum drying oven, 60 DEG C of drying under reduced pressure 5h, obtain the root of herbaceous peony, Radix Angelicae Sinensis, the root of Dahurain angelica dregs of a decoction respectively.Respectively with the distilled water 95 DEG C of 10,8 times amount (500ml, 400ml), extract 4h, in 80 DEG C of water-baths, be concentrated into 250ml.
Alcohol precipitation condition: root of herbaceous peony Aqueous extracts: add dehydrated alcohol slowly and make alcohol content reach 40% (V/V) in root of herbaceous peony concentrated solution, limit edged stirs, to put in frozen water 12 hours, 20 minutes (4000r/min) centrifugal treating, discard precipitation, retain this supernatant liquor, continue to add dehydrated alcohol and make alcohol content reach 60%; Radix Angelicae Sinensis Aqueous extracts: slowly add dehydrated alcohol make alcohol content reach 40% to Radix Angelicae Sinensis is concentrated, limit edged stirs, to put in frozen water 12 hours, 20 minutes (4000r/min) centrifugal treating, discard precipitation, retain this supernatant liquor, continue to add dehydrated alcohol and make alcohol content reach 80%; Root of Dahurain angelica Aqueous extracts: slowly add dehydrated alcohol and make alcohol content reach 40% in root of Dahurain angelica concentrated solution, limit edged stirs, and to put in frozen water 12 hours, and centrifugal removing precipitates, and retains this supernatant liquor, continues to add dehydrated alcohol and make alcohol content reach 85%.Respectively three kinds of solution to be put in frozen water 12 hours, careful removing supernatant liquor, lower floor uses centrifuge 20 minutes (4000r/min), retain polysaccharide precipitation, after using equimultiple acetone, washed with diethylether, centrifugal except desolventizing, precipitation is dissolved in 2 times amount distilled water, be placed in dialysis tubing (molecular weight cut-off < 6000) dialysis 24 hours, namely obtain root of herbaceous peony polysaccharide, Radix Angelicae Sinensis polysaccharide, angelica dahurica polysaccharide powder each about 2.30g, 1.84g, 0.90g through lyophilize.Get root of herbaceous peony polysaccharide, Radix Angelicae Sinensis polysaccharide, angelica dahurica polysaccharide powder 0.80g, 0.40g, 0.40g ground and mixed respectively evenly.
Embodiment 4
The extraction with the polysaccharide mixture of whitening function of the present invention:
Get the root of herbaceous peony, Radix Angelicae Sinensis, root of Dahurain angelica 200g respectively, 100g, 100g, be placed in 3 Erlenmeyer flasks respectively, add dehydrated alcohol 10 times amount, soak 24h, double gauze was considered, and discarded ethanol, and the dregs of a decoction continue to add dehydrated alcohol 8 times amount, soak 24h, double gauze was considered, and discarded ethanol, and the dregs of a decoction are placed in vacuum drying oven, 60 DEG C of drying under reduced pressure 5h, obtain the root of herbaceous peony, Radix Angelicae Sinensis, the root of Dahurain angelica dregs of a decoction respectively.Respectively with the distilled water 95 DEG C of 10,8 times amount (500ml, 400ml), extract 4h, in 80 DEG C of water-baths, be concentrated into 250ml.
Alcohol precipitation condition: root of herbaceous peony Aqueous extracts: add dehydrated alcohol slowly and make alcohol content reach 45% (volume ratio) in root of herbaceous peony concentrated solution, limit edged stirs, to put in frozen water 12 hours, 20 minutes (4000r/min) centrifugal treating, discard precipitation, retain this supernatant liquor, continue to add dehydrated alcohol and make alcohol content reach 65%; Radix Angelicae Sinensis Aqueous extracts: slowly add dehydrated alcohol make alcohol content reach 35% (volume ratio) to Radix Angelicae Sinensis is concentrated, limit edged stirs, to put in frozen water 12 hours, 20 minutes (4000r/min) centrifugal treating, discard precipitation, retain this supernatant liquor, continue to add dehydrated alcohol and make alcohol content reach 75%; Root of Dahurain angelica Aqueous extracts: slowly add dehydrated alcohol and make alcohol content reach 45% in root of Dahurain angelica concentrated solution, limit edged stirs, and to put in frozen water 12 hours, and centrifugal removing precipitates, and retains this supernatant liquor, continues to add dehydrated alcohol and make alcohol content reach 90%.Respectively three kinds of solution to be put in frozen water 12 hours, careful removing supernatant liquor, lower floor uses centrifuge 20 minutes (4000r/min), retain polysaccharide precipitation, after using equimultiple acetone, washed with diethylether, centrifugal except desolventizing, precipitation is dissolved in 2 times amount distilled water, be placed in dialysis tubing (molecular weight cut-off < 6000) dialysis 24 hours, namely obtain root of herbaceous peony polysaccharide, Radix Angelicae Sinensis polysaccharide, angelica dahurica polysaccharide powder each about 4.50g, 1.84g, 0.90g through lyophilize.Get root of herbaceous peony polysaccharide, Radix Angelicae Sinensis polysaccharide, angelica dahurica polysaccharide powder 5.00g, 2.00g, 1.00g ground and mixed respectively evenly.
The mixture of the present invention's each embodiment gained whitening function above-mentioned, can be made into various external application whitening class makeup, as external preparations such as emulsion, paste, liniment, whitening lotions.During use, with warm water by about this product 0.1g powder furnishing pasty state, be applied in face, skin.Facts have proved, the formation of the remarkable check melanin of Skin whitening care cosmetics energy of the present invention, the whitening function that tool is excellent.
The mixture effect of the invention described above gained whitening function measures:
Antioxygenation is assessed: the mixture of gained whitening function of the present invention adopts DPPH free radical scavenging effect working sample antioxygenation: take vitamins C as reference substance, when vitamin C concentration is 0.003mg/mL, clearance rate reaches 66 ± 4%, when the mixture concentration of whitening function is 0.2mg/mL, clearance rate can reach 58 ± 2%, illustrate that the mixture of gained whitening function of the present invention has certain effect of scavenging radical, there is antioxygenation;
Whitening function is assessed: tyrosinase inhibitory action, take arbutin as reference substance, the mixture of gained whitening function of the present invention and arbutin are under same concentrations during 0.5mg/mL, inhibiting rate is respectively 42 ± 3% and 49 ± 3%, illustrate that the mixture of gained whitening function of the present invention has stronger tyrosinase inhibitory action, whitening function is better.
The foregoing is only embodiments of the invention; not thereby the scope of the claims of the present invention is limited; every equivalents utilizing description of the present invention to do, or be directly or indirectly used in relevant technical field, be all in like manner included in scope of patent protection of the present invention.
Claims (6)
1. have a polysaccharide mixture for whitening function, it is characterized in that, the raw material comprising following weight part mixes: root of herbaceous peony polysaccharide 40-50 part; Radix Angelicae Sinensis polysaccharide 20-25 part; Angelica dahurica polysaccharide 20-25 part.
2. the polysaccharide mixture with whitening function according to claim 1, is characterized in that, the raw material comprising following weight part mixes: root of herbaceous peony polysaccharide 50 parts; Radix Angelicae Sinensis polysaccharide 25 parts; Angelica dahurica polysaccharide 25 parts.
3. one kind has the preparation method of the polysaccharide mixture of whitening function, it is characterized in that, comprise the steps: polysaccharide mixture root of herbaceous peony polysaccharide, Radix Angelicae Sinensis polysaccharide, angelica dahurica polysaccharide must after mixing by the part by weight of 40-50:20-25:20-25 with whitening function.
4. the preparation method with the polysaccharide mixture of whitening function according to claim 3, is characterized in that, the preparation method of described root of herbaceous peony polysaccharide comprises:
Get root of herbaceous peony root to pulverize, add soaked in absolute ethyl alcohol 24 hours, volume and the root of herbaceous peony root weight ratio of described dehydrated alcohol are 8:1; Filtration discards ethanol, and the gained dregs of a decoction continue to add soaked in absolute ethyl alcohol 24 hours, and volume and the root of herbaceous peony root weight ratio of described dehydrated alcohol are 6:1; Filtration discards ethanol, volatilizes ethanol after obtaining the dregs of a decoction;
The distilled water 95 DEG C again dregs of a decoction being added 10 times of weight ratios extracts 2 hours, and double gauze filters, and retains filtrate; The dregs of a decoction continue the distilled water adding 8 times of weight ratios, and 95 DEG C are extracted 2 hours, and double gauze filters, and retain filtrate; Merge twice gauze and filter rear gained filtrate, make filtrate in boiling water bath, be concentrated into 1/3rd of volume;
Concentrated rear filtrate is placed in 4 DEG C of ice-water baths, slowly adding dehydrated alcohol makes the percent by volume of alcohol content be 35-45%, limit edged stirs, be placed in 4 DEG C of frozen water after 12 hours, centrifugal removing precipitation, retain supernatant liquor, in supernatant liquor, add dehydrated alcohol afterwards again makes the percent by volume of alcohol content be 55-65%, be placed in 4 DEG C of frozen water and obtain solution A after 12 hours, removing solution A supernatant liquor obtains solution B, solution B uses centrifuge 20 minutes described centrifuge speeds to be 4000r/min, obtains the polysaccharide precipitation of solution B;
Described polysaccharide precipitation such as to use respectively at weight acetone, the washed with diethylether, centrifugal removing acetone and ether solvent after washing, gained precipitation is dissolved in the distilled water of its 2 times of weight ratios, be placed in dialysis tubing dialyse 24 hours, the molecular weight cut-off of described dialysis tubing is less than 6000, and gained retains the powder of liquid after lyophilize and is root of herbaceous peony polysaccharide.
5. the preparation method with the polysaccharide mixture of whitening function according to claim 3, is characterized in that, the preparation method of described Radix Angelicae Sinensis polysaccharide comprises:
Get angelica root to pulverize, add soaked in absolute ethyl alcohol 24 hours, volume and the root of herbaceous peony root weight ratio of described dehydrated alcohol are 8:1; Filtration discards ethanol, and the gained dregs of a decoction continue to add soaked in absolute ethyl alcohol 24 hours, and volume and the root of herbaceous peony root weight ratio of described dehydrated alcohol are 6:1; Filtration discards ethanol, volatilizes ethanol after obtaining the dregs of a decoction;
The distilled water 95 DEG C again dregs of a decoction being added 10 times of weight ratios extracts 2 hours, and double gauze filters, and retains filtrate; The dregs of a decoction continue the distilled water adding 8 times of weight ratios, and 95 DEG C are extracted 2 hours, and double gauze filters, and retain filtrate; Merge twice gauze and filter rear gained filtrate, make filtrate in boiling water bath, be concentrated into 1/3rd of volume;
Concentrated rear filtrate is placed in 4 DEG C of ice-water baths, slowly adding dehydrated alcohol makes the percent by volume of alcohol content be 35-45%, limit edged stirs, be placed in 4 DEG C of frozen water after 12 hours, centrifugal removing precipitation, retain supernatant liquor, in supernatant liquor, add dehydrated alcohol afterwards again makes the percent by volume of alcohol content be 75-85%, be placed in 4 DEG C of frozen water and obtain solution A after 12 hours, removing solution A supernatant liquor obtains solution B, solution B uses centrifuge 20 minutes described centrifuge speeds to be 4000r/min, obtains the polysaccharide precipitation of solution B;
Described polysaccharide precipitation such as to use respectively at weight acetone, the washed with diethylether, centrifugal removing acetone and ether solvent after washing, gained precipitation is dissolved in the distilled water of its 2 times of weight ratios, be placed in dialysis tubing dialyse 24 hours, the molecular weight cut-off of described dialysis tubing is less than 6000, and gained retains the powder of liquid after lyophilize and is Radix Angelicae Sinensis polysaccharide.
6. the preparation method with the polysaccharide mixture of whitening function according to claim 3, is characterized in that, the preparation method of described angelica dahurica polysaccharide comprises:
Get root of Dahurain angelica root to pulverize, add soaked in absolute ethyl alcohol 24 hours, volume and the root of herbaceous peony root weight ratio of described dehydrated alcohol are 8:1; Filtration discards ethanol, and the gained dregs of a decoction continue to add soaked in absolute ethyl alcohol 24 hours, and volume and the root of herbaceous peony root weight ratio of described dehydrated alcohol are 6:1; Filtration discards ethanol, volatilizes ethanol after obtaining the dregs of a decoction;
The distilled water 95 DEG C again dregs of a decoction being added 10 times of weight ratios extracts 2 hours, and double gauze filters, and retains filtrate; The dregs of a decoction continue the distilled water adding 8 times of weight ratios, and 95 DEG C are extracted 2 hours, and double gauze filters, and retain filtrate; Merge twice gauze and filter rear gained filtrate, make filtrate in boiling water bath, be concentrated into 1/3rd of volume;
Concentrated rear filtrate is placed in 4 DEG C of ice-water baths, slowly adding dehydrated alcohol makes the percent by volume of alcohol content be 45-55%, limit edged stirs, be placed in 4 DEG C of frozen water after 12 hours, centrifugal removing precipitation, retain supernatant liquor, in supernatant liquor, add dehydrated alcohol afterwards again makes the percent by volume of alcohol content be 80-90%, be placed in 4 DEG C of frozen water and obtain solution A after 12 hours, removing solution A supernatant liquor obtains solution B, solution B uses centrifuge 20 minutes described centrifuge speeds to be 4000r/min, obtains the polysaccharide precipitation of solution B;
Described polysaccharide precipitation such as to use respectively at weight acetone, the washed with diethylether, centrifugal removing acetone and ether solvent after washing, gained precipitation is dissolved in the distilled water of its 2 times of weight ratios, be placed in dialysis tubing dialyse 24 hours, the molecular weight cut-off of described dialysis tubing is less than 6000, and gained retains the powder of liquid after lyophilize and is angelica dahurica polysaccharide.
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