CN104829875B - Polysaccharide mixture with skin whitening effect and method for manufacturing polysaccharide mixture - Google Patents
Polysaccharide mixture with skin whitening effect and method for manufacturing polysaccharide mixture Download PDFInfo
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Abstract
The invention relates to the technical field of cosmetics, in particular to a polysaccharide mixture with a skin whitening effect and a method for manufacturing the polysaccharide mixture. The polysaccharide mixture with the skin whitening effect comprises, by weight, 40-50 parts of radix paeoniae alba polysaccharides, 20-25 parts of angelica sinensis polysaccharides and 20-25 parts of angelica dahurica polysaccharides. The radix paeoniae alba polysaccharides, the angelica sinensis polysaccharides and the angelica dahurica polysaccharides are mixed with one another to obtain the polysaccharide mixture. The polysaccharide mixture and the method have the advantages that ethanol precipitation conditions are controlled, so that ineffective polysaccharide ingredients in the total polysaccharides of radix paeoniae alba, angelica sinensis and angelica dahurica can be abandoned, polysaccharide active portions, which are capable of obviously suppressing tyrosinase, suppressing generation of melanin and realizing the excellent skin whitening effect, in the total polysaccharides can be screened, the purity of functional polysaccharides of the active portions can be greatly improved, the purity of active polysaccharide ingredients with skin whitening and anti-oxidation effects can be improved, and the activity of the polysaccharide mixture can be enhanced; active polysaccharide components extracted from three traditional Chinese medicinal materials which are the radix paeoniae alba, the angelica sinensis and the angelica dahurica are mixed with one another according to certain proportions and are coordinated with one another, so that the excellent effects can be realized.
Description
Technical field
The present invention relates to cosmetic technical field, and in particular to a kind of polysaccharide mixture with whitening function and its system
Preparation Method.
Background technology
Tryrosinase is the major limitation enzyme in melanin biosynthesis approach, and current whitening class cosmetics are mainly by suppression
Tryrosinase processed is reducing melanic formation in skin.Research whitening function, typically selects Tyrosinase inhibition assays conduct
Evaluate whitening effect generally to be received by numerous scholars.On market, whitening class cosmetics species is various, but contains mostly chemical composition,
Some adverse effects are there may be to skin, even there are carcinogenesis.The tyrosine kinase inhibitors in natural drug source, green,
Safety, not only for pigment type dermatosiss effectively, brightening, cosmetics formula is significant.
" Radix Paeoniae Alba polysaccharides are isolated and purified and property analysis. magazine biology [J], 2008,25 (5):Disclose in 34-36 "
Isolate and purify in Radix Paeoniae Alba water extraction solution and obtain a kind of polysaccharide protein complex, and prove which is homogeneous polysaccharide component；
" technical study of microwave extraction Radix Angelicae Sinensis whitening composition. daily chemical industry [J], 2004,34 (5):335-337”
In disclose microwave extraction Radix Angelicae Sinensis whitening composition, its whitening index is extract；
" experimentation of Radix Angelicae Dahuricae whitening lotion vitro inhibition tyrosinase activity. Changchun University of Traditional Chinese Medicine's journal [J],
2012,28 (6):The experimentation of Radix Angelicae Dahuricae whitening lotion vitro inhibition tyrosinase activity is disclosed in 960-961 ", is mentioned in text
In the preparation method of Radix Angelicae Dahuricae whitening lotion, effective ingredient is volatile oil；
From upper description, also there is research in prior art to the Radix Paeoniae Alba, Radix Angelicae Sinensis and the Radix Angelicae Dahuricae, but be concentrated mainly on the fortune of whole medicine
With concentrating on health food, medicine and the field such as extraction process, also have some herbal polysaccharide extracts of document report or its group
Compound has whitening function etc., but these herbal polysaccharide molecular weight ranges are larger, is a mixture, it may be said that be certain Chinese medicine
Total polysaccharidess, the composition that can wherein play whitening function or free radical scavenging function is certain part in total polysaccharidess, its
He is amount of activated too low or substantially without effect, and the total polysaccharidess of such Chinese medicine are due to simultaneously containing the active component and nothing for working
The non-active ingredient of effect and cause whitening function or free radical scavenging effect substantially reduce, play activity polysaccharide component purity
Also just it is greatly lowered.
The content of the invention
The technical problem to be solved is：A kind of preferable polysaccharide mixture of whitening function effect and its system are provided
Preparation Method.
In order to solve above-mentioned technical problem, the technical solution used in the present invention is：There is provided a kind of with many of whitening function
Sugared mixture, mixes including the raw material of following weight portion：Radix Paeoniae Alba polysaccharides 40-50 parts；Radix Angelicae Sinensis polysaccharide 20-25 parts；The Radix Angelicae Dahuricae is more
Sugared 20-25 parts.
Another technical scheme of the present invention is to provide a kind of preparation method of the polysaccharide mixture with whitening function, including
Following steps：Radix Paeoniae Alba polysaccharides, Radix Angelicae Sinensis polysaccharide, angelica dahurica polysaccharide are pressed into 40-50:20-25:After the part by weight of 20-25 is mixed
There must be the polysaccharide mixture of whitening function.
The beneficial effects of the present invention is：The present invention from the Radix Paeoniae Alba, Radix Angelicae Sinensis, Radix Angelicae Dahuricae total polysaccharidess, by control precipitate with ethanol condition,
Invalid polysaccharide component is discarded, tryrosinase in filtering out total polysaccharidess, can be significantly inhibited, suppress melanic production, whitening function
Preferably active polysaccharide part, greatly improves can the active part purity of polysaccharide that worked, make to be turned into whitening, antioxygen
Active polysaccharide material is improved, and then increased activity, and by the above-mentioned active polysaccharide extracted from three kinds of Chinese crude drugs
Component mixes by a certain percentage, serves mutually coordinated, the effect of reinforced effects.
Specific embodiment
By describing the technology contents of the present invention in detail, realizing purpose and effect, it is explained below in conjunction with embodiment.
The design of most critical of the present invention is：From the Radix Paeoniae Alba, Radix Angelicae Sinensis, Radix Angelicae Dahuricae total polysaccharidess, by controlling precipitate with ethanol condition, discard
Invalid polysaccharide component, plays the active polysaccharide part acted on whitening function or free radical scavenging in filtering out total polysaccharidess, and
Mix by a certain percentage, serve mutually coordinated, better effect.
The present invention provides a kind of polysaccharide mixture with whitening function, mixes including the raw material of following weight portion：
Radix Paeoniae Alba polysaccharides 40-50 parts；Radix Angelicae Sinensis polysaccharide 20-25 parts；Angelica dahurica polysaccharide 20-25 parts.
The preparation method of the above-mentioned polysaccharide mixture with whitening function comprises the steps：Will be Radix Paeoniae Alba polysaccharides, Radix Angelicae Sinensis many
Sugar, angelica dahurica polysaccharide are mixed by above-mentioned part by weight, the polysaccharide mixture must after grinding is uniform with whitening function.
In the preparation method of the above-mentioned polysaccharide mixture with whitening function, the preparation method of the Radix Paeoniae Alba polysaccharides includes：
The crushing of Radix Paeoniae Alba root, plus soaked in absolute ethyl alcohol 24 hours is taken, the volume of the dehydrated alcohol and Radix Paeoniae Alba root weight ratio are
8:1；Filtration discards ethanol, and gained medicinal residues continue plus soaked in absolute ethyl alcohol 24 hours, the volume of the dehydrated alcohol and the Radix Paeoniae Alba
Root weight ratio is 6:1；Filtration discards ethanol, volatilizes ethanol after obtaining medicinal residues；
95 DEG C of the distilled water of 10 times of weight ratios is added to extract 2 hours medicinal residues again, double gauze filtration retains filtrate；Medicinal residues
Continue to add the distilled water of 8 times of weight ratios, 95 DEG C are extracted 2 hours, double gauze filtration retains filtrate；Merge gauze filtration twice
Gained filtrate, makes filtrate be concentrated into 1/3rd of volume in boiling water bath afterwards；
Filtrate after concentration is placed in 4 DEG C of ice-water baths, after solution temperature is stable, being slowly added to dehydrated alcohol makes alcohol content
35-45% (V/V) is reached, it is stirring while adding, 4 DEG C of frozen water are placed in after 12 hours, are centrifuged off precipitation, retain supernatant, afterwards
In supernatant dehydrated alcohol is added to make alcohol content reach 55-65% (V/V) again, it is stirring while adding, it is placed in 4 DEG C of frozen water 12 little
When after solution A, remove solution A supernatant obtain solution B, solution B using 20 minutes centrifuge speeds of centrifuge is
4000r/min, obtains the polysaccharide precipitation of solution B；
The polysaccharide precipitation such as uses respectively at weight acetone, the ether washing, is centrifuged off acetone and ether solvent after washing,
Gained precipitation is dissolved in the distilled water of its 2 times of weight ratios, is placed in bag filter afterwards and is dialysed 24 hours, the retention of the bag filter
Molecular weight is less than 6000, and the powder after gained reservation liquid is freeze-dried is Radix Paeoniae Alba polysaccharides.
The preparation method of the Radix Angelicae Sinensis polysaccharide includes：
Angelica root crushing, plus soaked in absolute ethyl alcohol 24 hours is taken, the volume of the dehydrated alcohol and Radix Paeoniae Alba root weight ratio are
8:1；Filtration discards ethanol, and gained medicinal residues continue plus soaked in absolute ethyl alcohol 24 hours, the volume of the dehydrated alcohol and the Radix Paeoniae Alba
Root weight ratio is 6:1；Filtration discards ethanol, volatilizes ethanol after obtaining medicinal residues；
95 DEG C of the distilled water of 10 times of weight ratios is added to extract 2 hours medicinal residues again, double gauze filtration retains filtrate；Medicinal residues
Continue to add the distilled water of 8 times of weight ratios, 95 DEG C are extracted 2 hours, double gauze filtration retains filtrate；Merge gauze filtration twice
Gained filtrate, makes filtrate be concentrated into 1/3rd of volume in boiling water bath afterwards；
Filtrate after concentration is placed in 4 DEG C of ice-water baths, after solution temperature is stable, being slowly added to dehydrated alcohol makes alcohol content
35-45% (V/V) is reached, it is stirring while adding, 4 DEG C of frozen water are placed in after 12 hours, are centrifuged off precipitation, retain supernatant, afterwards
In supernatant dehydrated alcohol is added to make alcohol content reach 75-85% (V/V) again, it is stirring while adding, it is placed in 4 DEG C of frozen water 12 little
When after solution A, remove solution A supernatant obtain solution B, solution B using 20 minutes centrifuge speeds of centrifuge is
4000r/min, obtains the polysaccharide precipitation of solution B；
The polysaccharide precipitation such as uses respectively at weight acetone, the ether washing, is centrifuged off acetone and ether solvent after washing,
Gained precipitation is dissolved in the distilled water of its 2 times of weight ratios, is placed in bag filter afterwards and is dialysed 24 hours, the retention of the bag filter
Molecular weight is less than 6000, and the powder after gained reservation liquid is freeze-dried is Radix Angelicae Sinensis polysaccharide.
The preparation method of the angelica dahurica polysaccharide includes：
The crushing of Radix Angelicae Dahuricae root, plus soaked in absolute ethyl alcohol 24 hours is taken, the volume of the dehydrated alcohol and Radix Paeoniae Alba root weight ratio are
8:1；Filtration discards ethanol, and gained medicinal residues continue plus soaked in absolute ethyl alcohol 24 hours, the volume of the dehydrated alcohol and the Radix Paeoniae Alba
Root weight ratio is 6:1；Filtration discards ethanol, volatilizes ethanol after obtaining medicinal residues；
95 DEG C of the distilled water of 10 times of weight ratios is added to extract 2 hours medicinal residues again, double gauze filtration retains filtrate；Medicinal residues
Continue to add the distilled water of 8 times of weight ratios, 95 DEG C are extracted 2 hours, double gauze filtration retains filtrate；Merge gauze filtration twice
Gained filtrate, makes filtrate be concentrated into 1/3rd of volume in boiling water bath afterwards；
Filtrate after concentration is placed in 4 DEG C of ice-water baths, after solution temperature is stable, being slowly added to dehydrated alcohol makes alcohol content
45-55% is reached, it is stirring while adding, 4 DEG C of frozen water are placed in after 12 hours, are centrifuged off precipitation, retain supernatant, afterwards again upper
Add dehydrated alcohol to make alcohol content reach 80-90% in clear liquid, be placed in 4 DEG C of frozen water after 12 hours to obtain solution A, remove solution A
Supernatant obtains solution B, and solution B is 4000r/min using 20 minutes centrifuge speeds of centrifuge, obtains many of solution B
Sugar precipitation；
The polysaccharide precipitation such as uses respectively at weight acetone, the ether washing, is centrifuged off acetone and ether solvent after washing,
Gained precipitation is dissolved in the distilled water of its 2 times of weight ratios, is placed in bag filter afterwards and is dialysed 24 hours, the retention of the bag filter
Molecular weight is less than 6000, and the powder after gained reservation liquid is freeze-dried is angelica dahurica polysaccharide.
Embodiment 1
The present invention provides a kind of preparation method of the polysaccharide mixture with whitening function, comprises the steps：
Radix Paeoniae Alba polysaccharides preparation flow：Radix Paeoniae Alba dry root is taken, is crushed, shone as standard with crossing 80 mesh, plus 8 times of amounts of dehydrated alcohol
(V/W), soak 24 hours, filtration, discard ethanol, medicinal residues continue plus 6 times of amounts (V/W) of dehydrated alcohol, soak 24 hours, filter
Cross, discard ethanol, obtain medicinal residues, put under sunlight, naturally ventilated drying volatilizes (volatile dry) ethanol.Burning is put in medicinal residues transfer
In cup, plus 10 times of amount distilled water, 95 DEG C are extracted 2 hours, double gauze filtration, retain filtrate, and medicinal residues continue plus 8 times of amount distillations
Water, 95 DEG C are extracted 2 hours, and double gauze filtration, merging filtrate are concentrated into three points of filtrate volume in conical flask, in boiling water bath
One of, conical flask is placed in 4 DEG C of ice-water baths, liquid temp to be filtered is stable, slowly adds dehydrated alcohol to make alcohol content reach 35-
45%, it is stirring while adding, put 12 hours in frozen water, be centrifuged off precipitation, retain the supernatant, continuously adding dehydrated alcohol makes to contain
Alcohol amount reaches 55-65%, stirring while adding, puts 12 hours in frozen water, carefully removes supernatant, and lower floor uses centrifuge 20
Minute (4000r/min), retain polysaccharide precipitation, after being washed using equimultiple acetone, ether, be centrifuged off solvent, will precipitate molten
In 2 times of amount distilled water, dialysis 24 hours in bag filter (molecular cut off ＜ 6000) are placed in, freeze-dried to obtain final product the Radix Paeoniae Alba more
Icing Sugar end.
Radix Angelicae Sinensis polysaccharide preparation flow：Radix Angelicae Sinensis dry root is taken, is crushed, shone as standard with crossing 80 mesh, plus 8 times of amounts of dehydrated alcohol
(V/W), soak 24 hours, filtration, discard ethanol, medicinal residues continue plus 6 times of amounts (V/W) of dehydrated alcohol, soak 24 hours, filter
Cross, discard ethanol, obtain medicinal residues, put under sunlight, naturally ventilated drying volatilizes ethanol.Medicinal residues transfer is put in beaker, plus 10 times
Amount distilled water, 95 DEG C are extracted 2 hours, double gauze filtration, retain filtrate, and medicinal residues continue plus 8 times of amount distilled water, and 95 DEG C are extracted 2
Hour, double gauze filtration, merging filtrate is concentrated into 1/3rd of filtrate volume in conical flask, in boiling water bath, by taper
Bottle is placed in 4 DEG C of ice-water baths, and liquid temp to be filtered is stable, slowly adds dehydrated alcohol to make alcohol content reach 35-45%, and side edged is stirred
Mix, put 12 hours in frozen water, be centrifuged off precipitation, retain the supernatant, continuously adding dehydrated alcohol makes alcohol content reach 75-
85%, to put 12 hours in frozen water, carefully remove supernatant, lower floor uses centrifuge 20 minutes (4000r/min), retains many
Sugar precipitation, after washing using equimultiple acetone, ether, is centrifuged off solvent, and unique precipitation is dissolved in 2 times of amount distilled water, is placed in
In bag filter (molecular cut off ＜ 6000), dialysis 24 hours, freeze-dried to obtain final product Radix Angelicae Sinensis polysaccharide powder.
Angelica dahurica polysaccharide preparation flow：Radix Angelicae Dahuricae dry root is taken, is crushed, shone as standard with crossing 80 mesh, plus 8 times of amounts of dehydrated alcohol
(V/W), soak 24 hours, filtration, discard ethanol, medicinal residues continue plus 6 times of amounts (V/W) of dehydrated alcohol, soak 24 hours, filter
Cross, discard ethanol, obtain medicinal residues, put under sunlight, naturally ventilated drying volatilizes ethanol.Medicinal residues transfer is put in beaker, plus 10 times
Amount distilled water, 95 DEG C are extracted 2 hours, double gauze filtration, retain filtrate, and medicinal residues continue plus 8 times of amount distilled water, and 95 DEG C are extracted 2
Hour, double gauze filtration, merging filtrate is concentrated into 1/3rd of filtrate volume in conical flask, in boiling water bath, by taper
Bottle is placed in 4 DEG C of ice-water baths, and liquid temp to be filtered is stable, slowly adds dehydrated alcohol to make alcohol content reach 45-55%, and side edged is stirred
Mix, put 12 hours in frozen water, be centrifuged off precipitation, retain the supernatant, continuously adding dehydrated alcohol makes alcohol content reach 80-
90%, it is stirring while adding, to put 12 hours in frozen water, carefully remove supernatant, lower floor uses 20 minutes (4000r/ of centrifuge
Min), retain polysaccharide precipitation, after washing using equimultiple acetone, ether, be centrifuged off solvent, unique precipitation is dissolved in 2 times of amounts and steams
In distilled water, dialysis 24 hours in bag filter (molecular cut off ＜ 6000) are placed in, it is freeze-dried to obtain final product angelica dahurica polysaccharide powder.
The Radix Paeoniae Alba polysaccharides of above-mentioned preparation, Radix Angelicae Sinensis polysaccharide, angelica dahurica polysaccharide are pressed into 50%:25%:25% mass ratio is mixed,
Grinding is uniform, obtains final product the polysaccharide mixture with whitening function.
Embodiment 2
The Radix Paeoniae Alba, Radix Angelicae Sinensis, the Radix Angelicae Dahuricae 50g, 50g, 50g is taken respectively, is respectively placed in 3 conical flasks, plus 10 times of amounts of dehydrated alcohol,
Immersion 24h, double gauze considered, and discarded ethanol, and medicinal residues continuously add 8 times of amounts of dehydrated alcohol, soak 24h, and double gauze is considered
Cross, discard ethanol, medicinal residues are placed in vacuum drying oven, 60 DEG C of drying under reduced pressure 5h respectively obtain the Radix Paeoniae Alba, Radix Angelicae Sinensis, Radix Angelicae Dahuricae medicinal residues.Point
Not with 95 DEG C of the distilled water of 10,8 times of amounts (500ml, 400ml), 4h is extracted, in 80 DEG C of water-baths, 250ml is concentrated into.
Precipitate with ethanol condition：Radix Paeoniae Alba Aqueous extracts：To in Radix Paeoniae Alba concentrated solution, add dehydrated alcohol to make alcohol content reach 35-45% (V/ slowly
V), it is stirring while adding, to put 12 hours in frozen water, 20 minutes (4000r/min) centrifugal treating discard precipitation, retain the supernatant,
Continuously adding dehydrated alcohol makes alcohol content reach 55-65%；Radix Angelicae Sinensis Aqueous extracts：Dehydrated alcohol is slowly added to make containing alcohol to Radix Angelicae Sinensis concentration
Amount reaches 35-45% (V/V), stirring while adding, puts 12 hours in frozen water, 20 minutes (4000r/min) centrifugal treating, and it is heavy to discard
Form sediment, retain the supernatant, continuously adding dehydrated alcohol makes alcohol content reach 75-85%；Radix Angelicae Dahuricae Aqueous extracts：To in Radix Angelicae Dahuricae concentrated solution
Dehydrated alcohol is slowly added to make alcohol content reach 45-55%, it is stirring while adding, put 12 hours in frozen water, be centrifuged off precipitation, retain
The supernatant, continuously adding dehydrated alcohol makes alcohol content reach 80-90%.Respectively three kinds of solution are put 12 hours in frozen water, it is little
The heart remove supernatant, lower floor use centrifuge 20 minutes (4000r/min), retain polysaccharide precipitation, using equimultiple acetone,
After ether washing, solvent is centrifuged off, precipitation is dissolved in 2 times of amount distilled water, be placed in bag filter (molecular cut off ＜ 6000)
Middle dialysis 24 hours, it is freeze-dried obtain final product each about 1.15g of Radix Paeoniae Alba polysaccharides, Radix Angelicae Sinensis polysaccharide, angelica dahurica polysaccharide powder, 0.92g,
0.45g.It is uniform that Radix Paeoniae Alba polysaccharides, Radix Angelicae Sinensis polysaccharide, angelica dahurica polysaccharide powder 0.5g, 0.1g, 0.1g ground and mixed is taken respectively.
Embodiment 3
The extraction of the polysaccharide mixture with whitening function of the present invention：
The Radix Paeoniae Alba, Radix Angelicae Sinensis, the Radix Angelicae Dahuricae 100g, 100g, 100g is taken respectively, is respectively placed in 3 conical flasks, plus 10 times of dehydrated alcohol
Amount, soaks 24h, and double gauze was considered, and discards ethanol, and medicinal residues continuously add 8 times of amounts of dehydrated alcohol, soak 24h, double gauze
Considered, discarded ethanol, medicinal residues were placed in vacuum drying oven, 60 DEG C of drying under reduced pressure 5h respectively obtained the Radix Paeoniae Alba, Radix Angelicae Sinensis, Radix Angelicae Dahuricae medicinal residues.
Respectively with 95 DEG C of the distilled water of 10,8 times of amounts (500ml, 400ml), 4h is extracted, in 80 DEG C of water-baths, 250ml is concentrated into.
Precipitate with ethanol condition：Radix Paeoniae Alba Aqueous extracts：To in Radix Paeoniae Alba concentrated solution, add dehydrated alcohol to make alcohol content reach 40% (V/V) slowly,
It is stirring while adding, to put 12 hours in frozen water, 20 minutes (4000r/min) centrifugal treating discard precipitation, retain the supernatant, after
It is continuous to add dehydrated alcohol to make alcohol content reach 60%；Radix Angelicae Sinensis Aqueous extracts：Dehydrated alcohol is slowly added to reach alcohol content to Radix Angelicae Sinensis concentration
It is to 40%, stirring while adding, to put 12 hours in frozen water, 20 minutes (4000r/min) centrifugal treating discard precipitation, retain on this
Clear liquid, continuously adding dehydrated alcohol makes alcohol content reach 80%；Radix Angelicae Dahuricae Aqueous extracts：Slowly add dehydrated alcohol in Radix Angelicae Dahuricae concentrated solution
Alcohol content is made to reach 40%, it is stirring while adding, put 12 hours in frozen water, be centrifuged off precipitation, retain the supernatant, continuously add
Dehydrated alcohol makes alcohol content reach 85%.Respectively three kinds of solution are put 12 hours in frozen water, supernatant is carefully removed, lower floor uses
Centrifuge 20 minutes (4000r/min), retains polysaccharide precipitation, after being washed using equimultiple acetone, ether, is centrifuged off molten
Agent, precipitation is dissolved in 2 times of amount distilled water, is placed in dialysis 24 hours in bag filter (molecular cut off ＜ 6000), chilled dry
It is dry to obtain final product Radix Paeoniae Alba polysaccharides, Radix Angelicae Sinensis polysaccharide, angelica dahurica polysaccharide powder each about 2.30g, 1.84g, 0.90g.Radix Paeoniae Alba polysaccharides, Radix Angelicae Sinensis are taken respectively
Polysaccharide, angelica dahurica polysaccharide powder 0.80g, 0.40g, 0.40g ground and mixed are uniform.
Embodiment 4
The extraction of the polysaccharide mixture with whitening function of the present invention：
The Radix Paeoniae Alba, Radix Angelicae Sinensis, the Radix Angelicae Dahuricae 200g, 100g, 100g is taken respectively, is respectively placed in 3 conical flasks, plus 10 times of dehydrated alcohol
Amount, soaks 24h, and double gauze was considered, and discards ethanol, and medicinal residues continuously add 8 times of amounts of dehydrated alcohol, soak 24h, double gauze
Considered, discarded ethanol, medicinal residues were placed in vacuum drying oven, 60 DEG C of drying under reduced pressure 5h respectively obtained the Radix Paeoniae Alba, Radix Angelicae Sinensis, Radix Angelicae Dahuricae medicinal residues.
Respectively with 95 DEG C of the distilled water of 10,8 times of amounts (500ml, 400ml), 4h is extracted, in 80 DEG C of water-baths, 250ml is concentrated into.
Precipitate with ethanol condition：Radix Paeoniae Alba Aqueous extracts：To in Radix Paeoniae Alba concentrated solution, add dehydrated alcohol to make alcohol content reach 45% (volume slowly
Than), it is stirring while adding, to put 12 hours in frozen water, 20 minutes (4000r/min) centrifugal treating discard precipitation, retain the supernatant
Liquid, continuously adding dehydrated alcohol makes alcohol content reach 65%；Radix Angelicae Sinensis Aqueous extracts：Dehydrated alcohol is slowly added to make containing alcohol to Radix Angelicae Sinensis concentration
Amount reaches 35% (volume ratio), stirring while adding, puts 12 hours in frozen water, 20 minutes (4000r/min) centrifugal treating, and it is heavy to discard
Form sediment, retain the supernatant, continuously adding dehydrated alcohol makes alcohol content reach 75%；Radix Angelicae Dahuricae Aqueous extracts：Delay in Radix Angelicae Dahuricae concentrated solution
Slowly plus dehydrated alcohol makes alcohol content reach 45%, it is stirring while adding, put 12 hours in frozen water, be centrifuged off precipitation, retain on this
Clear liquid, continuously adding dehydrated alcohol makes alcohol content reach 90%.Respectively three kinds of solution are put 12 hours in frozen water, it is careful to remove
Clear liquid, lower floor use centrifuge 20 minutes (4000r/min), retain polysaccharide precipitation, are washed using equimultiple acetone, ether
Afterwards, solvent is centrifuged off, precipitation is dissolved in 2 times of amount distilled water, be placed in dialysis 24 in bag filter (molecular cut off ＜ 6000)
Hour, it is freeze-dried to obtain final product Radix Paeoniae Alba polysaccharides, Radix Angelicae Sinensis polysaccharide, angelica dahurica polysaccharide powder each about 4.50g, 1.84g, 0.90g.Take respectively
Radix Paeoniae Alba polysaccharides, Radix Angelicae Sinensis polysaccharide, angelica dahurica polysaccharide powder 5.00g, 2.00g, 1.00g ground and mixed are uniform.
The mixture of whitening function obtained by above-mentioned each embodiment of the present invention, can be fabricated to various external whitening classes and make up
The external preparation such as product, such as Emulsion, unguentum, liniment, whitening lotion.During use, about this product 0.1g powder is tuned into into pasty state with warm water, is applied
Smear in face, skin.It was verified that the Skin whitening care cosmeticses of the present invention can significantly inhibit melanic formation, has excellent whitening
Effect.
The mixture effect of whitening function obtained by the invention described above is determined：
Antioxidation is assessed：The mixture of gained whitening function of the invention determines sample using the effect of DPPH free radical scavengings
Product antioxidation：With vitamin C as reference substance, when vitamin C concentration is 0.003mg/mL, clearance rate reaches 66 ± 4%, U.S.
When the mixture concentration of white effect is 0.2mg/mL, clearance rate can reach 58 ± 2%, illustrate the mixed of gained whitening function of the invention
Compound has certain effect of scavenging radical, with antioxidation；
Whitening function is assessed：Tyrosinase inhibitory action, with arbutin as reference substance, present invention gained whitening function it is mixed
, under same concentrations during 0.5mg/mL, suppression ratio is respectively 42 ± 3% and 49 ± 3%, illustrates institute of the present invention for compound and arbutin
The mixture for obtaining whitening function has stronger tyrosinase inhibitory action, and whitening function is preferable.
Embodiments of the invention are the foregoing is only, the scope of the claims of the present invention is not thereby limited, it is every using this
The equivalents made by bright description, or the technical field of correlation is directly or indirectly used in, this is included in the same manner
In bright scope of patent protection.
Claims (3)
1. a kind of polysaccharide mixture with whitening function, it is characterised in that mix including the raw material of following weight portion：In vain
Chinese herbaceous peony polysaccharide 40-50 parts；Radix Angelicae Sinensis polysaccharide 20-25 parts；Angelica dahurica polysaccharide 20-25 parts；The preparation method of the Radix Paeoniae Alba polysaccharides includes：
The crushing of Radix Paeoniae Alba root, plus soaked in absolute ethyl alcohol 24 hours is taken, the volume of the dehydrated alcohol and Radix Paeoniae Alba root weight ratio are 8:1；
Filtration discards ethanol, and gained medicinal residues continue plus soaked in absolute ethyl alcohol 24 hours, the volume of the dehydrated alcohol and Radix Paeoniae Alba root weight
Amount is than being 6:1；Filtration discards ethanol, volatilizes ethanol after obtaining medicinal residues；
95 DEG C of the distilled water of 10 times of weight ratios is added to extract 2 hours medicinal residues again, double gauze filtration retains filtrate；Medicinal residues continue
Plus the distilled water of 8 times of weight ratios, 95 DEG C are extracted 2 hours, double gauze filtration, retain filtrate；Merge institute after gauze filtration twice
Filtrate is obtained, makes filtrate be concentrated into 1/3rd of volume in boiling water bath；
Filtrate after concentration is placed in 4 DEG C of ice-water baths, it is 35- to be slowly added to dehydrated alcohol and make the percentage by volume of alcohol content
45%, it is stirring while adding, 4 DEG C of frozen water are placed in after 12 hours, are centrifuged off precipitation, retain supernatant, afterwards again in supernatant
It is 55-65% to add dehydrated alcohol to make the percentage by volume of alcohol content, is placed in 4 DEG C of frozen water after 12 hours to obtain solution A, removes molten
Liquid A supernatant obtains solution B, and solution B is 4000r/min using 20 minutes centrifuge speeds of centrifuge, obtains solution B
Polysaccharide precipitation；
The polysaccharide precipitation such as uses respectively at weight acetone, the ether washing, is centrifuged off acetone and ether solvent, gained after washing
Precipitation is dissolved in the distilled water of its 2 times of weight ratios, is placed in bag filter afterwards and is dialysed 24 hours, the retention molecule of the bag filter
Less than 6000, the powder after gained reservation liquid is freeze-dried is Radix Paeoniae Alba polysaccharides to amount；
The preparation method of the Radix Angelicae Sinensis polysaccharide includes：
Angelica root crushing, plus soaked in absolute ethyl alcohol 24 hours is taken, the volume of the dehydrated alcohol and angelica root weight ratio are 8:1；
Filtration discards ethanol, and gained medicinal residues continue plus soaked in absolute ethyl alcohol 24 hours, the volume of the dehydrated alcohol and angelica root weight
Amount is than being 6:1；Filtration discards ethanol, volatilizes ethanol after obtaining medicinal residues；
95 DEG C of the distilled water of 10 times of weight ratios is added to extract 2 hours medicinal residues again, double gauze filtration retains filtrate；Medicinal residues continue
Plus the distilled water of 8 times of weight ratios, 95 DEG C are extracted 2 hours, double gauze filtration, retain filtrate；Merge institute after gauze filtration twice
Filtrate is obtained, makes filtrate be concentrated into 1/3rd of volume in boiling water bath；
Filtrate after concentration is placed in 4 DEG C of ice-water baths, it is 35- to be slowly added to dehydrated alcohol and make the percentage by volume of alcohol content
45%, it is stirring while adding, 4 DEG C of frozen water are placed in after 12 hours, are centrifuged off precipitation, retain supernatant, afterwards again in supernatant
It is 75-85% to add dehydrated alcohol to make the percentage by volume of alcohol content, is placed in 4 DEG C of frozen water after 12 hours to obtain solution A, removes molten
Liquid A supernatant obtains solution B, and solution B is 4000r/min using 20 minutes centrifuge speeds of centrifuge, obtains solution B
Polysaccharide precipitation；
The polysaccharide precipitation such as uses respectively at weight acetone, the ether washing, is centrifuged off acetone and ether solvent, gained after washing
Precipitation is dissolved in the distilled water of its 2 times of weight ratios, is placed in bag filter afterwards and is dialysed 24 hours, the retention molecule of the bag filter
Less than 6000, the powder after gained reservation liquid is freeze-dried is Radix Angelicae Sinensis polysaccharide to amount；
The preparation method of the angelica dahurica polysaccharide includes：
The crushing of Radix Angelicae Dahuricae root, plus soaked in absolute ethyl alcohol 24 hours is taken, the volume of the dehydrated alcohol and Radix Angelicae Dahuricae root weight ratio are 8:1；
Filtration discards ethanol, and gained medicinal residues continue plus soaked in absolute ethyl alcohol 24 hours, the volume of the dehydrated alcohol and Radix Angelicae Dahuricae root weight
Amount is than being 6:1；Filtration discards ethanol, volatilizes ethanol after obtaining medicinal residues；
95 DEG C of the distilled water of 10 times of weight ratios is added to extract 2 hours medicinal residues again, double gauze filtration retains filtrate；Medicinal residues continue
Plus the distilled water of 8 times of weight ratios, 95 DEG C are extracted 2 hours, double gauze filtration, retain filtrate；Merge institute after gauze filtration twice
Filtrate is obtained, makes filtrate be concentrated into 1/3rd of volume in boiling water bath；
Filtrate after concentration is placed in 4 DEG C of ice-water baths, it is 45- to be slowly added to dehydrated alcohol and make the percentage by volume of alcohol content
55%, it is stirring while adding, 4 DEG C of frozen water are placed in after 12 hours, are centrifuged off precipitation, retain supernatant, afterwards again in supernatant
It is 80-90% to add dehydrated alcohol to make the percentage by volume of alcohol content, is placed in 4 DEG C of frozen water after 12 hours to obtain solution A, removes molten
Liquid A supernatant obtains solution B, and solution B is 4000r/min using 20 minutes centrifuge speeds of centrifuge, obtains solution B
Polysaccharide precipitation；
The polysaccharide precipitation such as uses respectively at weight acetone, the ether washing, is centrifuged off acetone and ether solvent, gained after washing
Precipitation is dissolved in the distilled water of its 2 times of weight ratios, is placed in bag filter afterwards and is dialysed 24 hours, the retention molecule of the bag filter
Less than 6000, the powder after gained reservation liquid is freeze-dried is angelica dahurica polysaccharide to amount.
2. the polysaccharide mixture with whitening function according to claim 1, it is characterised in that including following weight portion
Raw material is mixed：50 parts of Radix Paeoniae Alba polysaccharides；25 parts of Radix Angelicae Sinensis polysaccharide；25 parts of angelica dahurica polysaccharide.
3. a kind of preparation method of the polysaccharide mixture with whitening function, it is characterised in that comprise the steps：The Radix Paeoniae Alba is more
Sugar, Radix Angelicae Sinensis polysaccharide, angelica dahurica polysaccharide press 40-50:20-25:The part by weight of 20-25 must have many of whitening function after being mixed
Sugared mixture；The preparation method of the Radix Paeoniae Alba polysaccharides includes：
The crushing of Radix Paeoniae Alba root, plus soaked in absolute ethyl alcohol 24 hours is taken, the volume of the dehydrated alcohol and Radix Paeoniae Alba root weight ratio are 8:1；
Filtration discards ethanol, and gained medicinal residues continue plus soaked in absolute ethyl alcohol 24 hours, the volume of the dehydrated alcohol and Radix Paeoniae Alba root weight
Amount is than being 6:1；Filtration discards ethanol, volatilizes ethanol after obtaining medicinal residues；
95 DEG C of the distilled water of 10 times of weight ratios is added to extract 2 hours medicinal residues again, double gauze filtration retains filtrate；Medicinal residues continue
Plus the distilled water of 8 times of weight ratios, 95 DEG C are extracted 2 hours, double gauze filtration, retain filtrate；Merge institute after gauze filtration twice
Filtrate is obtained, makes filtrate be concentrated into 1/3rd of volume in boiling water bath；
Filtrate after concentration is placed in 4 DEG C of ice-water baths, it is 35- to be slowly added to dehydrated alcohol and make the percentage by volume of alcohol content
45%, it is stirring while adding, 4 DEG C of frozen water are placed in after 12 hours, are centrifuged off precipitation, retain supernatant, afterwards again in supernatant
It is 55-65% to add dehydrated alcohol to make the percentage by volume of alcohol content, is placed in 4 DEG C of frozen water after 12 hours to obtain solution A, removes molten
Liquid A supernatant obtains solution B, and solution B is 4000r/min using 20 minutes centrifuge speeds of centrifuge, obtains solution B
Polysaccharide precipitation；
The polysaccharide precipitation such as uses respectively at weight acetone, the ether washing, is centrifuged off acetone and ether solvent, gained after washing
Precipitation is dissolved in the distilled water of its 2 times of weight ratios, is placed in bag filter afterwards and is dialysed 24 hours, the retention molecule of the bag filter
Less than 6000, the powder after gained reservation liquid is freeze-dried is Radix Paeoniae Alba polysaccharides to amount；
The preparation method of the Radix Angelicae Sinensis polysaccharide includes：
Angelica root crushing, plus soaked in absolute ethyl alcohol 24 hours is taken, the volume of the dehydrated alcohol and angelica root weight ratio are 8:1；
Filtration discards ethanol, and gained medicinal residues continue plus soaked in absolute ethyl alcohol 24 hours, the volume of the dehydrated alcohol and angelica root weight
Amount is than being 6:1；Filtration discards ethanol, volatilizes ethanol after obtaining medicinal residues；
95 DEG C of the distilled water of 10 times of weight ratios is added to extract 2 hours medicinal residues again, double gauze filtration retains filtrate；Medicinal residues continue
Plus the distilled water of 8 times of weight ratios, 95 DEG C are extracted 2 hours, double gauze filtration, retain filtrate；Merge institute after gauze filtration twice
Filtrate is obtained, makes filtrate be concentrated into 1/3rd of volume in boiling water bath；
Filtrate after concentration is placed in 4 DEG C of ice-water baths, it is 35- to be slowly added to dehydrated alcohol and make the percentage by volume of alcohol content
45%, it is stirring while adding, 4 DEG C of frozen water are placed in after 12 hours, are centrifuged off precipitation, retain supernatant, afterwards again in supernatant
It is 75-85% to add dehydrated alcohol to make the percentage by volume of alcohol content, is placed in 4 DEG C of frozen water after 12 hours to obtain solution A, removes molten
Liquid A supernatant obtains solution B, and solution B is 4000r/min using 20 minutes centrifuge speeds of centrifuge, obtains solution B
Polysaccharide precipitation；
The polysaccharide precipitation such as uses respectively at weight acetone, the ether washing, is centrifuged off acetone and ether solvent, gained after washing
Precipitation is dissolved in the distilled water of its 2 times of weight ratios, is placed in bag filter afterwards and is dialysed 24 hours, the retention molecule of the bag filter
Less than 6000, the powder after gained reservation liquid is freeze-dried is Radix Angelicae Sinensis polysaccharide to amount；
The preparation method of the angelica dahurica polysaccharide includes：
The crushing of Radix Angelicae Dahuricae root, plus soaked in absolute ethyl alcohol 24 hours is taken, the volume of the dehydrated alcohol and Radix Angelicae Dahuricae root weight ratio are 8:1；
Filtration discards ethanol, and gained medicinal residues continue plus soaked in absolute ethyl alcohol 24 hours, the volume of the dehydrated alcohol and Radix Angelicae Dahuricae root weight
Amount is than being 6:1；Filtration discards ethanol, volatilizes ethanol after obtaining medicinal residues；
95 DEG C of the distilled water of 10 times of weight ratios is added to extract 2 hours medicinal residues again, double gauze filtration retains filtrate；Medicinal residues continue
Plus the distilled water of 8 times of weight ratios, 95 DEG C are extracted 2 hours, double gauze filtration, retain filtrate；Merge institute after gauze filtration twice
Filtrate is obtained, makes filtrate be concentrated into 1/3rd of volume in boiling water bath；
Filtrate after concentration is placed in 4 DEG C of ice-water baths, it is 45- to be slowly added to dehydrated alcohol and make the percentage by volume of alcohol content
55%, it is stirring while adding, 4 DEG C of frozen water are placed in after 12 hours, are centrifuged off precipitation, retain supernatant, afterwards again in supernatant
It is 80-90% to add dehydrated alcohol to make the percentage by volume of alcohol content, is placed in 4 DEG C of frozen water after 12 hours to obtain solution A, removes molten
Liquid A supernatant obtains solution B, and solution B is 4000r/min using 20 minutes centrifuge speeds of centrifuge, obtains solution B
Polysaccharide precipitation；
The polysaccharide precipitation such as uses respectively at weight acetone, the ether washing, is centrifuged off acetone and ether solvent, gained after washing
Precipitation is dissolved in the distilled water of its 2 times of weight ratios, is placed in bag filter afterwards and is dialysed 24 hours, the retention molecule of the bag filter
Less than 6000, the powder after gained reservation liquid is freeze-dried is angelica dahurica polysaccharide to amount.
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| CN110403850B (en) * | 2019-08-19 | 2021-10-22 | 华南理工大学 | Polysaccharide whitening solid mask and preparation method and application thereof |
| CN111803400A (en) * | 2020-08-18 | 2020-10-23 | 黑龙江中医药大学 | Traditional Chinese medicine polysaccharide moisturizing and freckle removing mask and preparation method thereof |
| CN116421525B (en) * | 2023-05-06 | 2023-09-19 | 甄萃(广东)创新技术有限公司 | Paeonia root extract and preparation method thereof |
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| CN1762324A (en) * | 2004-10-22 | 2006-04-26 | 卢藏田 | Composite whitening speckle-removing rehabilitation cosmetic |
| CN101125119A (en) * | 2007-07-18 | 2008-02-20 | 西藏宇妥藏药研究有限公司 | Tibetan materia medica with whitening anti-senility function and application thereof |
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Address after: 241000, No. 18, Shanxi Road, Wuhu District, Yijiang, Anhui Patentee after: ANHUI College OF TRADITIONAL CHINESE MEDICINE Address before: 241000, No. 18, Shanxi Road, Wu Xia Road, Ge Jiang District, Anhui, Wuhu Patentee before: ANHUI COLLEGE OF TRADITIONAL CHINESE MEDICINE |
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