CN104825528A - Composition capable of increasing bone mineral density, and preparation method and application thereof - Google Patents
Composition capable of increasing bone mineral density, and preparation method and application thereof Download PDFInfo
- Publication number
- CN104825528A CN104825528A CN201510217181.8A CN201510217181A CN104825528A CN 104825528 A CN104825528 A CN 104825528A CN 201510217181 A CN201510217181 A CN 201510217181A CN 104825528 A CN104825528 A CN 104825528A
- Authority
- CN
- China
- Prior art keywords
- compositions
- cervi
- fructus
- cucumidis sativi
- filtrate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Abstract
The invention relates to a composition capable of increasing bone mineral density, and a preparation method and application thereof. The active ingredient of the composition is mainly prepared from the following raw materials by weight: 20 to 30 parts of deer bone, 15 to 20 parts of cucumber seed and 15 to 20 parts of epimedium. The composition is capable of tonifying the kidney, reinforcing bones, invigorating blood circulation to dredge collaterals and removing dampness to relieve pain and exerts good curative effects when used for prevention and treatment of osteoporosis.
Description
Technical field
The present invention relates to technical field of Chinese medicines, particularly relate to a kind of composition and method of making the same and the application with increase bone density effect.
Background technology
Osteoporosis (osteoporosis) is one group of osteopathia that many reasons causes, and osseous tissue has normal calcification, and calcium salt and substrate are normal rates, and the metabolic osteopathy being reduced to feature with unit volume inner bone tissues amount becomes.In most osteoporosis, caused by the minimizing of osseous tissue increases mainly due to bone absorption.How slow morbidity is, individually comparatively fast, with skeleton pain, be easy to fracture for feature, and biochemical analysis is normal.The visible cortical bone of pathological anatomy is belittled, and the sparse atrophy of bone trabecula, osteoid layer is not thick.
At present, for osteoporotic medicine mainly estrogen and other drug auxiliary treatment, bone density and the related symptoms thereof of osteoporosis patient can be improved.
But in recent years, it is found that Western medicine toxic and side effects in treatment osteoporosis is large, cure the symptoms, not the disease, and conventionally have dependency and side effect.Therefore research and develop one and can treat osteoporotic Chinese medicine safely and effectively, there is extraordinary practical value.
Summary of the invention
Based on this, the invention discloses a kind of compositions with increase bone density effect, said composition has the effect strengthening bone density, can be used for prevention and therapy osteoporosis.
Have the compositions increasing bone density effect, the effective ingredient of described compositions is prepared from primarily of the raw material of following weight portion: Os Cervi 20-30 part, Semen Cucumidis sativi 13-20 part, Herba Epimedii 15-20 part.
Above-mentioned have in the compositions increasing bone density effect, and Os Cervi is the skeleton of animal in deer family Cervus nippon Temminck (CervusNippon Temmick); Semen Cucumidis sativi is the seed of cucurbitaceous plant Fructus Cucumidis sativi; Herba Epimedii is Berberidaceae barrenwort, is rich in flavones ingredient.Above-mentioned composition has the effect of invigorating the kidney and strengthening the bones, Huoxue San " network, damp-clearing pain-relieving, can alleviate lumbago and backache, whole body osteodynia, and can improve microcirculation, promotes doped calcium, increases lumbar vertebra and femoral neck bone density, can be used for prevention and therapy osteoporosis.
Wherein in an embodiment, the effective ingredient of described compositions is prepared from by the raw material of following weight portion: Os Cervi 20-30 part, Semen Cucumidis sativi 13-20 part, Herba Epimedii 15-20 part, Fructus Psoraleae 12-15 part, Cortex Eucommiae 12-15 part, Fructus Lycii 10-15 part, Fructus Schisandrae Chinensis 10-15 part, Semen Cuscutae 5-8 part, Fructus Cnidii 5-8 part, Fructus Corni 2-5 part, Radix Angelicae Sinensis 2-5 part.
In above-mentioned composition, except Os Cervi, Semen Cucumidis sativi and Herba Epimedii, also select plurality of Chinese composition synergism, wherein, Fructus Psoraleae is the seed of the annual upright herbaceous plant Fructus Psoraleae of Rosales pulse family, has the function of kidney invigorating and YANG supporting, spleen reinforcing stomach invigorating; The Cortex Eucommiae is the dry bark of the Rosales Eucommiaceae plant Cortex Eucommiae, has liver and kidney tonifying, bone and muscle strengthening, Chong and Ren Meridians regulating, admittedly through antiabortive effect; The mature fruit that Fructus Lycii is matrimony vine of solanaceae plant, has nourishing the liver and kidney, effect of replenishing vital essence to improve eyesight; The dry mature fruit that Fructus Schisandrae Chinensis is magnoliaceae schisandra, has convergence astringent or styptic treatment for spontaneous sweating, supplementing QI for promoting the production of body fluid, effect of kidney calming; Semen Cuscutae is the seed of the annual parasitic herbaceous plant Semen Cuscutae of tubular flower order Convolvulaceae, has the kidney invigorating and essence nourishing, effect of nourishing the liver to improve visual acuity; Fructus Cnidii is the dry mature fruit of samphire cnidium monnieri, has warming the kidney to invigorate YANG, and dampness is dispeled the wind, effect of parasite killing; Fructus Corni is the mature fruit of Umbellales Cornaceae plant Fructus Corni, has liver and kidney tonifying; Effect that convergence is solid; When being classified as the root of Umbellales Umbelliferae herbaceos perennial Radix Angelicae Sinensis, having and enriching blood; Invigorate blood circulation; Menstruction regulating and pain relieving; Moisturize effect of laxation.
In prescription, Os Cervi sweet in the mouth, slight fever, it is thin to be good at tonify deficiency, the kidney warming sun, bone and muscle strengthening; Herba Epimedii acrid-sweet flavor, is longer than kidney invigorating and YANG supporting, expelling wind and removing dampness; Semen Cucumidis sativi: reunion of fractured tendons and bones.The high kidney-replenishing of three tastes, strong muscles and bones are monarch drug altogether.
Compatibility Fructus Psoraleae, kidney invigorating and YANG supporting, spleen reinforcing stomach invigorating; The Cortex Eucommiae, liver and kidney tonifying, bone and muscle strengthening; Fructus Lycii, nourishing the liver and kidney, replenishing vital essence to improve eyesight; Semen Cuscutae, the kidney invigorating and essence nourishing, nourishing the liver to improve visual acuity; Fructus Corni, liver and kidney tonifying; Restrain astringent or styptic treatment for spontaneous sweating; Fructus Cnidii, warming the kidney to invigorate YANG.Above-mentioned five tastes nourishing the liver and kidney, mutual generation between metal and water, strong water, with the fire that helps, is ministerial drug altogether.
Radix Angelicae Sinensis, enriches blood, invigorates blood circulation, pain relieving, is adjuvant drug.
Fructus Schisandrae Chinensis, restrains astringent or styptic treatment for spontaneous sweating, supplementing QI for promoting the production of body fluid, for making medicine.
Take a broad view of full side, treating both the principal and secondary aspects of a disease, tonifying both YIN and YANG, drugs for nourishing yin is suitable with warming YANG medicine, and coordinating water and fire, bone strengthening can the phase.
Wherein in an embodiment, the effective ingredient of described compositions is prepared from by the raw material of following weight portion: Os Cervi 23-27 part, Semen Cucumidis sativi 15-18 part, Herba Epimedii 15-17 part, Fructus Psoraleae 13-14 part, Cortex Eucommiae 13-14 part, Fructus Lycii 12-13 part, Fructus Schisandrae Chinensis 12-13 part, Semen Cuscutae 6-7 part, Fructus Cnidii 6-7 part, Fructus Corni 3-4 part, Radix Angelicae Sinensis 3-4 part.Each component is combined according to said ratio, there is best increase bone density effect.
Wherein in an embodiment, described Os Cervi prepares Os Cervi polypeptide by the following method, with the effective ingredient of Os Cervi polypeptide as compositions:
(1) extract: get Os Cervi, doubly add water by the 2-6 of Os Cervi weight, at 1.3-1.4 atmospheric pressure, extract 2-5 hour under the condition of 120-126 DEG C, remove upper strata oils and fats after leaving standstill, filter to get filtrate, filtering residue extracts 0-3 time by preceding method again, merging filtrate, for subsequent use;
(2) enzymolysis: be the pepsin that 1:30-70 adds 3000-4000u/g according to the mass ratio of enzyme and Os Cervi dry weight in filtrate obtained above, regulates filtrate pH value to 1.0-3.0, enzymolysis 4-6 hour at 35-40 DEG C; Be the trypsin that 1:30-70 adds 3500-4500u/g according to the mass ratio of enzyme and Os Cervi dry weight again, regulate filtrate pH value to 7.0-9.0, enzymolysis 4-6 hour at 40-50 DEG C; Be warming up to 85-95 DEG C, keep 10-20min, inactivation treatment is carried out to enzyme, obtains Os Cervi polypeptide.Understandable, after obtaining above-mentioned enzymolysis solution, can also filter, 60 DEG C of vacuum concentration are to 1/5 of stock solution volume, and spraying dry, can obtain character preferably Os Cervi polypeptide dry product.
Prepare Os Cervi polypeptide by said method, there is good degree of hydrolysis, and comparatively close with human internal environment, meet Chinese medicine theory.
Wherein in an embodiment, described Semen Cucumidis sativi prepares Semen Cucumidis sativi polypeptide by the following method, with the effective ingredient of Os Cervi polypeptide as compositions:
(1) extract: get Semen Cucumidis sativi, doubly add water by the 2-6 of Semen Cucumidis sativi weight, under the condition of 80-100 DEG C, extract 1-3 hour, filter to get filtrate, filtering residue extracts 0-3 time by preceding method again, and merging filtrate is for subsequent use;
(2) enzymolysis: be the trypsin that 1:30-70 adds 3500-4500u/g according to the mass ratio of enzyme and Semen Cucumidis sativi dry weight in filtrate obtained above, regulates filtrate pH value to 7.0-9.0, enzymolysis 4-6 hour at 40-50 DEG C.Be warming up to 85-95 DEG C, keep 10-20min, inactivation treatment is carried out to enzyme, obtains Semen Cucumidis sativi polypeptide.Understandable, after obtaining above-mentioned enzymolysis solution, can also filter, 60 DEG C of vacuum concentration are to 1/5 of stock solution volume, and spraying dry, can obtain character preferably Semen Cucumidis sativi polypeptide dry product.
Prepare Semen Cucumidis sativi polypeptide by said method, there is good degree of hydrolysis, and comparatively close with human internal environment, meet Chinese medicine theory.
The invention also discloses a kind of above-mentioned preparation method with the compositions increasing bone density effect, comprise the following steps:
The extraction of Herba Epimedii: take Herba Epimedii by recipe quantity, the solid-liquid ratio adding 10-14ml solvent according to every gram of raw material adds the ethanol that concentration expressed in percentage by volume is 50-95%, reflux, extract, 1-4 time, each extraction 0.5-2 hour, above extracting solution is merged, filter, recycling design, after drying, obtain Herba Epimedii extract;
The preparation of extractum: take Fructus Psoraleae, the Cortex Eucommiae, Fructus Lycii, Fructus Schisandrae Chinensis, Semen Cuscutae, Fructus Cnidii, Fructus Corni, Radix Angelicae Sinensis by recipe quantity, the solid-liquid ratio adding 6-10ml solvent according to every gram of raw material adds water, decoct and extract 1-4 time, each 1-2 hour, above extracting solution is merged, filter, recycling design to relative density is 1.05-1.15, adding ethanol to containing alcohol mass percentage is 65-75%, precipitate with ethanol 12-36 hour, filters, decompression filtrate recycling ethanol, and be evaporated to the thick paste that relative density is 1.20-1.25, obtain extractum after drying;
Mixing: get Os Cervi polypeptide obtained above, Semen Cucumidis sativi polypeptide, and get Herba Epimedii extract obtained above and extractum, mix homogeneously, to obtain final product.
Chinese medicine combines with modern extraction technique by the present invention, can retain Effective Component of Chinese Medicine to greatest extent, can refine again to Chinese medicine, changes the presentation of its " black, large, thick ", can improve acceptance and the compliance of people.And it is reasonable that above-mentioned preparation method has craft science, and steady quality, controlled, convenient operation, can realize industrialization continuous seepage, the advantage that production cost is low.
The invention also discloses and a kind ofly above-mentioned there is the compositions that increases bone density effect for the preparation of the application improved in osteoporotic medicine or health food.
Wherein in an embodiment, the dosage form of described medicine or health food is tablet, granule, hard capsule, soft capsule, oral liquid, pill or drop pill.
Above-mentioned dosage form can adopt method well known to those skilled in the art to carry out the preparation of medicament.As required, various pharmaceutically acceptable carrier can be added.Described carrier comprises the diluent, excipient, filler, binding agent, wetting agent, disintegrating agent, absorption enhancer, surfactant, absorption carrier, lubricant etc. of pharmaceutical field routine.In the preparation, selectable filler includes but not limited to medicament of the present invention: starch, Icing Sugar, calcium phosphate, dextrin, microcrystalline Cellulose, lactose, pregelatinized Starch, mannitol etc.; Selectable binding agent includes but not limited to: sodium carboxymethyl cellulose, PVP-K30, hydroxypropyl cellulose, starch slurry, methylcellulose, ethyl cellulose, hydroxypropyl emthylcellulose, gelling starch etc.; Selectable disintegrating agent includes but not limited to: dried starch, polyvinylpolypyrrolidone, cross-linking sodium carboxymethyl cellulose, carboxymethyl starch sodium, low-substituted hydroxypropyl cellulose etc.; Selectable lubricant includes but not limited to: magnesium stearate, Pulvis Talci, sodium lauryl sulphate, micropowder silica gel etc.
Compared with prior art, the present invention has following beneficial effect:
A kind of compositions with increase bone density effect of the present invention, primarily of Os Cervi, Semen Cucumidis sativi, Herba Epimedii composition, has the effect of invigorating the kidney and strengthening the bones, Huoxue San " network, damp-clearing pain-relieving, for prevention and therapy osteoporosis, has good curative effect.
Particularly when said composition is made up of Os Cervi, Semen Cucumidis sativi, Herba Epimedii, Fructus Psoraleae, the Cortex Eucommiae, Fructus Lycii, Fructus Schisandrae Chinensis, Semen Cuscutae, Fructus Cnidii, Fructus Corni and Radix Angelicae Sinensis, there is best increase bone density effect.
A kind of preparation method with the compositions increasing bone density effect of the present invention, adopt modern Chinese medicine extractive technique, have craft science reasonable, steady quality, controlled, convenient operation, can realize industrialization continuous seepage, the feature that production cost is low.
Detailed description of the invention
The present invention is described further by the following examples, but do not cause any restriction to the present invention.
Embodiment 1
There is the compositions increasing bone density effect, take each component by following formula:
Os Cervi 25g, Semen Cucumidis sativi 15g, Herba Epimedii 15g, Fructus Psoraleae 13g, Cortex Eucommiae 13g, Fructus Lycii 13g, Fructus Schisandrae Chinensis 13g, Semen Cuscutae 6g, Fructus Cnidii 6g, Fructus Corni 3g, Radix Angelicae Sinensis 3g.
Preparation technology is as follows:
One, the preparation of Os Cervi polypeptide.
1, extract.
Get Os Cervi, be cut into segment, clean water three times, the fur of removing adhesion and bulk fat, add the water of 4 times amount, at 1.3-1.4 atmospheric pressure, extract 3 hours under the condition of 120-126 DEG C, take out Os Cervi, hold over night, remove upper strata oils and fats, the fine and closely woven filtered through gauze of multilamellar, filtrate stores for future use.Residue is continued the water adding 3 times amount, by aforementioned condition High Temperature High Pressure 3 hours, take out Os Cervi, hold over night, removing upper strata oils and fats, the fine and closely woven filtered through gauze of multilamellar, filtrate and front first-time filtrate merge, and store for future use.
2, enzymolysis.
1) screening of enzyme.
First activation characteristics according to enzyme carries out single enzymolysis to 5 kinds of enzymes, take degree of hydrolysis as screening index, and using the size of degree of hydrolysis as preferred foundation, parallel processing 2 increment product, results averaged, as shown in the table.
The hydrolysising condition of table 15 kinds of protease and result
Wherein, ratio at the bottom of enzyme is the mass ratio of enzyme and Os Cervi dry weight.
As can be seen from upper table result, trypsin and alkaline protease are optimum enzyme, but consider human internal environment simultaneously, finally determine that optimum enzyme is that the two enzyme of pepsin and trypsin carries out enzymolysis.
2) screening of enzymatic hydrolysis condition.
Single factor exploration is carried out to pepsin, trypsin digestion, take degree of hydrolysis as screening index, and using degree of hydrolysis size as preferred foundation, the repairing effect after simultaneously osteoblast being damaged with reference to pharmacodynamic index enzymatic hydrolysate, the optimum enzymatic hydrolysis condition finally determined is as follows:
Pepsin enzymatic hydrolysis condition: than being 1:50 at the bottom of enzyme, pH value is 2.0, and the time is 5 hours, and temperature is 37 DEG C.
Trypsin digestion condition is: than being 1:50 at the bottom of enzyme, pH value is 8.0, and the time is 5 hours, and temperature is 45 DEG C.
Thus, carry out enzymolysis by the following method in the present embodiment: in filtrate obtained above according to enzyme at the bottom of ratio (mass ratio of enzyme and Os Cervi dry weight) add the pepsin of 3500u/g for 1:50, regulate filtrate pH value to 2.0, enzymolysis 5 hours at 37 DEG C; Again according to ratio at the bottom of enzyme (mass ratio of enzyme and Os Cervi dry weight) for 1:50 adds 4000u/g trypsin, regulate filtrate pH value to 8.0, enzymolysis 5 hours at 45 DEG C.Be warming up to 90 DEG C, keep 15min, inactivation treatment is carried out to enzyme.Be cooled to room temperature, filter, 60 DEG C of vacuum concentration are to 1/5 of stock solution volume, and spraying dry, obtains Os Cervi polypeptide.
Above-mentioned enzymatic hydrolysate is done MTT experiment on osteoblast verify, mean absorbance values is 0.7685.
Two, the preparation of Semen Cucumidis sativi polypeptide.
1, extract.
Get Semen Cucumidis sativi, add water by 4 times of Semen Cucumidis sativi weight, extract 2 hours under the condition of 100 DEG C, filter to get filtrate, filtering residue extracts 2 times again by preceding method, and merging filtrate is for subsequent use.
2, enzymolysis.
According to the method described above enzyme not being carried out not and the screening of enzymatic hydrolysis condition to Semen Cucumidis sativi, determining that optimum enzymolysis condition is: than being 1:50 at the bottom of enzyme, pH value is 8.0, and the time is 5 hours, and temperature is 45 DEG C.
Thus, carry out the enzymolysis of Semen Cucumidis sativi in the present embodiment by the following method: in filtrate obtained above according to enzyme at the bottom of ratio (mass ratio of enzyme and Semen Cucumidis sativi dry weight) add the trypsin of 4000u/g for 1:50, regulate filtrate pH value to 8.0, enzymolysis 5 hours at 45 DEG C.Be warming up to 90 DEG C, keep 15min, inactivation treatment is carried out to enzyme.Be cooled to room temperature, filter, 60 DEG C of vacuum concentration are to 1/5 of stock solution volume, and spraying dry, obtains Semen Cucumidis sativi polypeptide.
Three, the extraction of Herba Epimedii.
Herba Epimedii is taken by recipe quantity, according to solid-liquid ratio (g/ml) for 1:12 adds the ethanol that concentration expressed in percentage by volume is 70%, reflux, extract, 3 times, each extraction 1 hour, merges above extracting solution, filter, recycling design, obtains Herba Epimedii extract after spraying dry, by analysis, assert that in extract, main matter is Herba Epimedii total flavones and icariin, extraction ratio is 20.08%.
Four, the preparation of extractum.
Fructus Psoraleae, the Cortex Eucommiae, Fructus Lycii, Fructus Schisandrae Chinensis, Semen Cuscutae, Fructus Cnidii, Fructus Corni, Radix Angelicae Sinensis is taken by recipe quantity, according to solid-liquid ratio (g/ml) for 1:8 adds water, soak 3 hours, decoct 1.5 hours, then filter, filtering residue is that 1:6 adds water according to solid-liquid ratio again, decocts 1 hour, filter, merging filtrate.
Filtrate reduced in volume (pressure 0.08MPa, temperature 70 C) to the relative density above-mentioned water extraction obtained is 1.10 (60 DEG C of surveys); Add 95% ethanol to alcohol content 70%, precipitate with ethanol 24 hours, filter, decompression filtrate recycling ethanol (pressure 0.05Mpa, 60 DEG C), concentrating under reduced pressure (pressure 0.08MPa, temperature 60 C) to relative density is the thick paste of 1.20-1.25 (60 DEG C of heat are surveyed); Thick paste drying under reduced pressure (pressure 0.08Mpa, temperature 70 C), obtains extractum, for subsequent use.
This extractum is Fructus Psoraleae, the Cortex Eucommiae, Fructus Lycii, Fructus Schisandrae Chinensis, Semen Cuscutae, Fructus Cnidii, Fructus Corni, Radix Angelicae Sinensis extract (extraction ratio: 11.0%).
Five, mix.
Get Os Cervi polypeptide obtained above, Semen Cucumidis sativi polypeptide, and get Herba Epimedii extract obtained above and extractum, mix homogeneously, obtain compositions A.
Embodiment 2
Have the compositions increasing bone density effect, substantially identical with the compositions of embodiment 1, difference is that its formula is as follows:
Os Cervi 20g, Semen Cucumidis sativi 20g, Herba Epimedii 15g, Fructus Psoraleae 15g, Cortex Eucommiae 12g, Fructus Lycii 15g, Fructus Schisandrae Chinensis 10g, Semen Cuscutae 8g, Fructus Cnidii 5g, Fructus Corni 2g, Radix Angelicae Sinensis 5g.
Compositions B is prepared according to the method for embodiment 1.
Embodiment 3
Have the compositions increasing bone density effect, substantially identical with the compositions of embodiment 1, difference is that its formula is as follows:
Os Cervi 30g, Semen Cucumidis sativi 13g, Herba Epimedii 20g, Fructus Psoraleae 12g, Cortex Eucommiae 15g, Fructus Lycii 10g, Fructus Schisandrae Chinensis 15g, Semen Cuscutae 5g, Fructus Cnidii 8g, Fructus Corni 5g, Radix Angelicae Sinensis 2g.
Compositions C is prepared according to the method for embodiment 1.
Embodiment 4
There is the tablet increasing bone density effect, prepare by the following method:
The compositions A that Example 1 prepares, adds appropriate amount of starch, dextrin, then adds 95% ethanol 18 mesh sieve granulation, and room temperature dries granulate (20 mesh sieve), and add appropriate magnesium stearate, Pulvis Talci, tabletting, coating is finished product.
Comparative example 1
A kind of compositions, substantially identical with the compositions of embodiment 1, difference is that its formula is as follows:
Os Cervi 25g, Semen Cucumidis sativi 15g.
Compositions D is prepared according to the method for embodiment 1.
Comparative example 2
A kind of compositions, substantially identical with the compositions of embodiment 1, difference is that its formula is as follows:
Herba Epimedii 15g, Fructus Psoraleae 13g, Cortex Eucommiae 13g, Fructus Lycii 13g, Fructus Schisandrae Chinensis 13g, Semen Cuscutae 6g, Fructus Cnidii 6g, Fructus Corni 3g, Radix Angelicae Sinensis 3g.
Compositions E is prepared according to the method for embodiment 1.
Comparative example 3
A kind of compositions, substantially identical with the compositions of embodiment 1, difference is that its formula is as follows:
Carapax et Plastrum Testudinis 5g, Semen Cucumidis sativi 15g, Herba Epimedii 15g, Fructus Psoraleae 13g, Cortex Eucommiae 13g, Fructus Lycii 13g, Fructus Schisandrae Chinensis 13g, Semen Cuscutae 6g, Fructus Cnidii 6g, Fructus Corni 3g, Radix Angelicae Sinensis 3g.
Prepare according to the method for embodiment 1, wherein Carapax et Plastrum Testudinis adopts the preparation method of Os Cervi to prepare tortoise plastron polypeptide, obtains composition F.
Comparative example 4
A kind of compositions, substantially identical with the compositions of embodiment 1, difference is that its formula is as follows:
Os Cervi 10g, Semen Cucumidis sativi 10g, Herba Epimedii 10g, Fructus Psoraleae 10g, Cortex Eucommiae 8g, Fructus Lycii 8g, Fructus Schisandrae Chinensis 8g, Semen Cuscutae 15g, Fructus Cnidii 15g, Fructus Corni 10g, Radix Angelicae Sinensis 10g.
Compositions G is prepared according to the method for embodiment 1.
Experimental example
Compositions above-described embodiment and comparative example prepared carries out effect experimental.
One, primary condition is tested.
1) laboratory animal: SPF level SD rat, female, Jilin University's medical experiment animal center provides.
2) rearing conditions: indoor maintenance 25 ± 1 DEG C, freely drinks water, takes food, and adopts 12h:12h intermittent illumination round the clock, changes weekly water 3 times, change bedding and padding 2 times, and water material is sufficient, and SPF level is raised.
2), experimental result statistical procedures: experimental data adopts Microsoft Excel 2003 to carry out statistical procedures, and result represents with x ± s, and carries out TTEST analysis, and t value method compares the significance of group difference.
Two, experimental technique.
Choosing healthy SD rat, is female entirely, body weight 180-220g, and animal is quarantined about one week after coming; Then modeling process is carried out.
Modeling method is: rat is through lumbar injection chloral hydrate 10%, anaesthetize by 0.3g/kg body weight, in ventrimeson apart from vaginal orifice 3-4cm place unhairing after abdomen position is fixing, use iodine tincture and alcohol disinfecting respectively, skin is cut and abdominal muscle is about 2-3cm after slightly dry, otch visual field visible white fat, push aside after fat deposit finds uterus, gently side cornua uteri is pulled out, at its end as seen by ovary that liparitosis is wrapped up, fractionation of fatty group, just the ovary of pink or yellowish red color can be seen, ovary is clamped with mosquito forceps, then fallopian tube under ovary (comprising fat) is used silk thread ligation, wipe out ovary (check whether and wipe out completely), take advantage of a situation and cornua uteri is sent back in abdominal cavity, another side ovary is wiped out with method.Abdominal muscle and skin layering are sterilized after sewing up again.Finally by hindlimb muscle injection 4000U/kg penicillin, continuous antibiotics process 3 days.
Rat, after 3 days, divides into groups by removal ovary at random, is respectively Normal group, model control group, compositions A-G group; Only often organize 15-16.Respectively each group of rat is weighed, numbering, and carry out administration by the dosage in table 1.
Rat records weekly body weight once, and by body weight value adjustment dosage.This model experiment time is 4 months.Each group of rat, after 4 months, is all measured BMD value, femur weight in wet base, wet density, the dry density of bone density by modeling.
Three, observation index
1, bone densitometry
Dual-energy X-ray, measures neck of femur, femur distal end and femur mid point with borne densitometers.First the femur of rat the same side is scanned, choose femoral head to kneed position; Then carry out data analysis: first measure femur total length, then determine mid point, survey the bone density BMD value within the scope of 8mm × 11mm; Then equal area is copied in neck of femur and femoral far heart end position, record BMD value.
2, femur weight in wet base, wet density, dry density measure
Take out right side femur, pick the connective tissue of most attachment, after analytical balance is weighed, micro-scale volume determinator measures volume, and calculate wet density (weight in wet base/volume).Dry in 105 DEG C of baking ovens to constant weight, weigh, calculate dry density (dry weight/volume).
Four, experimental result
Table 1 given the test agent affects result to ovariectomized female rats bone density BMD's.(x±SD)
Note: compare * P < 0.05 with the model control group same period; * P < 0.01; * * P < 0.001.
Can be obtained by table 1 experimental result, the neck of femur of model control group rat and the bone density of femur distal end obviously reduce, compared with same period Normal group, there is significant difference (P < 0.001), prompting is owing to directly causing the estrogenic reduction of laboratory animal after removal ovary, make bone metabolism be that negative balance-bone resorption strengthens, bone amount is lost gradually, and osteoporosis model is successful on the whole.
Compositions A, B, C group all can increase the neck of femur of ovariectomized female rats and the BMD value of femoral joint significantly, the neck of femur of compositions A, B, C group and the BMD value of femoral joint are compared with same period model control group, all there is significant difference (P < 0.01), in sum: compositions A, B, C group all have good effect to the bone density increase of ovariectomized female rats.And compositions A group has best enhancing bone density effect.
Compositions D-G group, although also there is certain enhancing bone density effect, compared with same period model control group, do not have difference (P ﹥ 0.05) statistically, therefore its effect is not as compositions A-C group.
Table 2 given the test agent affects result (x ± SD) to ovariectomized female rats body weight, bone wet density and dry density
Note: compare * P < 0.05 with the model control group same period; * P < 0.01; * * P < 0.001.
From table 2 experimental result, femur wet density and the dry density value of model control group rat all obviously reduce, compared with same period Normal group, there is significant difference (P < 0.05, P < 0.001), and the body weight of model control group rat obviously increases, and there is significant difference (P < 0.01) same period compared with Normal group; When the weight of femur increases (weight in wet base and dry weight), volume increase and be in the state of estrogen deficiency, there is the specific variations of femur wet density and dry density in table 2, illustrated that ovariectomized female rats has formed typical osteoporosis model when reaching 4 months thus.
Compositions A, B, C group all can increase femur wet density and the dry density of ovariectomized female rats, the femoral shaft density value of compositions A, B, C group is compared with same period model control group, there is significant difference (P < 0.001, P < 0.01); In sum, compositions A, the bone density increase of B, C group to ovariectomized female rats have good effect.And compositions A group has best enhancing bone density effect.
Compositions D-G group, although also there is certain enhancing bone density effect, compared with same period model control group, do not have difference (P ﹥ 0.05) statistically, therefore its effect is not as compositions A-C group.
Above-mentioned experimental result shows, the compositions that the embodiment of the present invention prepares has particularly preferred increase bone density, treats osteoporotic effect.
The above embodiment only have expressed several embodiment of the present invention, and it describes comparatively concrete and detailed, but can not therefore be construed as limiting the scope of the patent.It should be pointed out that for the person of ordinary skill of the art, without departing from the inventive concept of the premise, can also make some distortion and improvement, these all belong to protection scope of the present invention.Therefore, the protection domain of patent of the present invention should be as the criterion with claims.
Claims (8)
1. have the compositions increasing bone density effect, it is characterized in that, the effective ingredient of described compositions is prepared from primarily of the raw material of following weight portion:
Os Cervi 20-30 part, Semen Cucumidis sativi 13-20 part, Herba Epimedii 15-20 part.
2. according to claim 1 have the compositions increasing bone density effect, and it is characterized in that, the effective ingredient of described compositions is prepared from by the raw material of following weight portion:
Os Cervi 20-30 part, Semen Cucumidis sativi 13-20 part, Herba Epimedii 15-20 part, Fructus Psoraleae 12-15 part, Cortex Eucommiae 12-15 part, Fructus Lycii 10-15 part, Fructus Schisandrae Chinensis 10-15 part, Semen Cuscutae 5-8 part, Fructus Cnidii 5-8 part, Fructus Corni 2-5 part, Radix Angelicae Sinensis 2-5 part.
3. according to claim 1 have the compositions increasing bone density effect, and it is characterized in that, the effective ingredient of described compositions is prepared from by the raw material of following weight portion:
Os Cervi 23-27 part, Semen Cucumidis sativi 15-18 part, Herba Epimedii 15-17 part, Fructus Psoraleae 13-14 part, Cortex Eucommiae 13-14 part, Fructus Lycii 12-13 part, Fructus Schisandrae Chinensis 12-13 part, Semen Cuscutae 6-7 part, Fructus Cnidii 6-7 part, Fructus Corni 3-4 part, Radix Angelicae Sinensis 3-4 part.
4. the compositions with increase bone density effect according to any one of claim 1-3, it is characterized in that, described Os Cervi is prepared into Os Cervi polypeptide by the following method, with the effective ingredient of Os Cervi polypeptide as compositions:
(1) extract: get Os Cervi, doubly add water by the 2-6 of Os Cervi weight, at 1.3-1.4 atmospheric pressure, extract 2-5 hour under the condition of 120-126 DEG C, remove upper strata oils and fats after leaving standstill, filter to get filtrate, filtering residue extracts 0-3 time by preceding method again, merging filtrate, for subsequent use;
(2) enzymolysis: be the pepsin that 1:30-70 adds 3000-4000u/g according to the mass ratio of enzyme and Os Cervi dry weight in filtrate obtained above, regulates filtrate pH value to 1.0-3.0, enzymolysis 4-6 hour at 35-40 DEG C; Be the trypsin that 1:30-70 adds 3500-4500u/g according to the mass ratio of enzyme and Os Cervi dry weight again, regulate filtrate pH value to 7.0-9.0, enzymolysis 4-6 hour at 40-50 DEG C; Be warming up to 85-95 DEG C, keep 10-20min, inactivation treatment is carried out to enzyme, obtains Os Cervi polypeptide.
5. the compositions with increase bone density effect according to any one of claim 1-3, it is characterized in that, described Semen Cucumidis sativi is prepared into Semen Cucumidis sativi polypeptide by the following method, with the effective ingredient of Os Cervi polypeptide as compositions:
(1) extract: get Semen Cucumidis sativi, doubly add water by the 2-6 of Semen Cucumidis sativi weight, under the condition of 80-100 DEG C, extract 1-3 hour, filter to get filtrate, filtering residue extracts 0-3 time by preceding method again, and merging filtrate is for subsequent use;
(2) enzymolysis: be the trypsin that 1:30-70 adds 3500-4500u/g according to the mass ratio of enzyme and Semen Cucumidis sativi dry weight in filtrate obtained above, regulates filtrate pH value to 7.0-9.0, enzymolysis 4-6 hour at 40-50 DEG C.Be warming up to 85-95 DEG C, keep 10-20min, inactivation treatment is carried out to enzyme, obtains Semen Cucumidis sativi polypeptide.
6. the preparation method with the compositions increasing bone density effect described in any one of claim 2-5, is characterized in that, comprise the following steps:
The extraction of Herba Epimedii: take Herba Epimedii by recipe quantity, the solid-liquid ratio adding 10-14ml solvent according to every gram of raw material adds the ethanol that concentration expressed in percentage by volume is 50-95%, reflux, extract, 1-4 time, each extraction 0.5-2 hour, above extracting solution is merged, filter, recycling design, after drying, obtain Herba Epimedii extract;
The preparation of extractum: take Fructus Psoraleae, the Cortex Eucommiae, Fructus Lycii, Fructus Schisandrae Chinensis, Semen Cuscutae, Fructus Cnidii, Fructus Corni, Radix Angelicae Sinensis by recipe quantity, the solid-liquid ratio adding 6-10ml solvent according to every gram of raw material adds water, decoct and extract 1-4 time, each 1-2 hour, above extracting solution is merged, filter, recycling design to relative density is 1.05-1.15, adding ethanol to containing alcohol mass percentage is 65-75%, precipitate with ethanol 12-36 hour, filters, decompression filtrate recycling ethanol, and be evaporated to the thick paste that relative density is 1.20-1.25, obtain extractum after drying;
Mixing: get Os Cervi polypeptide that claim 4 prepares, Semen Cucumidis sativi polypeptide that claim 5 prepares, and get Herba Epimedii extract obtained above and extractum, mix homogeneously, to obtain final product.
7. the compositions that having described in any one of claim 1-5 increases bone density effect is for the preparation of the application improved in osteoporotic medicine or health food.
8. compositions according to claim 7 is for the preparation of the application improved in osteoporotic medicine or health food, it is characterized in that, the dosage form of described medicine or health food is tablet, granule, hard capsule, soft capsule, oral liquid, pill or drop pill.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510217181.8A CN104825528B (en) | 2015-04-30 | 2015-04-30 | Have effects that increase the composition and its preparation method and application of bone density |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510217181.8A CN104825528B (en) | 2015-04-30 | 2015-04-30 | Have effects that increase the composition and its preparation method and application of bone density |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104825528A true CN104825528A (en) | 2015-08-12 |
| CN104825528B CN104825528B (en) | 2019-01-11 |
Family
ID=53803979
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510217181.8A Expired - Fee Related CN104825528B (en) | 2015-04-30 | 2015-04-30 | Have effects that increase the composition and its preparation method and application of bone density |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104825528B (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105412903A (en) * | 2015-12-03 | 2016-03-23 | 东莞广州中医药大学中医药数理工程研究院 | Medicine for treating osteoporosis and preparation technology of medicine |
| CN107495349A (en) * | 2017-09-29 | 2017-12-22 | 武汉华士特工业生物技术开发有限公司 | A kind of functional food composition of increase bone density containing maca and green plum and preparation method thereof |
| CN107625124A (en) * | 2017-09-29 | 2018-01-26 | 武汉华士特工业生物技术开发有限公司 | A kind of functional food for increasing bone density and preparation method thereof |
| CN109289036A (en) * | 2018-10-26 | 2019-02-01 | 湛江市通灵医学生物工程有限公司 | Compound preparation and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1843502A (en) * | 2006-01-16 | 2006-10-11 | 广东医学院 | Active bone peptide for treating and preventing osteoporosis and its preparing method |
| CN101015580A (en) * | 2006-12-12 | 2007-08-15 | 广东医学院 | pH-dependent composite bone peptide preparation, preparing method and use thereof |
| CN102988607A (en) * | 2012-11-24 | 2013-03-27 | 王萍 | Health-care traditional Chinese medicine composition for osteoporosis |
| CN103349674A (en) * | 2013-08-05 | 2013-10-16 | 吉林农业大学 | Tortoise shell rana japonica oil composition for increasing bone density and preventing osteoporosis |
-
2015
- 2015-04-30 CN CN201510217181.8A patent/CN104825528B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1843502A (en) * | 2006-01-16 | 2006-10-11 | 广东医学院 | Active bone peptide for treating and preventing osteoporosis and its preparing method |
| CN101015580A (en) * | 2006-12-12 | 2007-08-15 | 广东医学院 | pH-dependent composite bone peptide preparation, preparing method and use thereof |
| CN102988607A (en) * | 2012-11-24 | 2013-03-27 | 王萍 | Health-care traditional Chinese medicine composition for osteoporosis |
| CN103349674A (en) * | 2013-08-05 | 2013-10-16 | 吉林农业大学 | Tortoise shell rana japonica oil composition for increasing bone density and preventing osteoporosis |
Non-Patent Citations (5)
| Title |
|---|
| 刘义等: "鹿骨瓜子肽胶囊促进骨细胞增殖及其毒理稳定性研究", 《中国新药杂志》 * |
| 刘雄伟等: "中西医结合治疗肾阳虚型骨质疏松症60例疗效观察", 《湖南中医杂志》 * |
| 张鹤等: "鹿骨胶原蛋白对去卵巢所致骨质疏松大鼠的治疗作用", 《中药药理与临床》 * |
| 彭成等主编: "《中药药理学》", 31 December 2014, 中国医药科技出版社 * |
| 蔡伟明等: "黄瓜籽多肽的抗骨质疏松作用", 《中国医院药学杂志》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105412903A (en) * | 2015-12-03 | 2016-03-23 | 东莞广州中医药大学中医药数理工程研究院 | Medicine for treating osteoporosis and preparation technology of medicine |
| CN107495349A (en) * | 2017-09-29 | 2017-12-22 | 武汉华士特工业生物技术开发有限公司 | A kind of functional food composition of increase bone density containing maca and green plum and preparation method thereof |
| CN107625124A (en) * | 2017-09-29 | 2018-01-26 | 武汉华士特工业生物技术开发有限公司 | A kind of functional food for increasing bone density and preparation method thereof |
| CN109289036A (en) * | 2018-10-26 | 2019-02-01 | 湛江市通灵医学生物工程有限公司 | Compound preparation and preparation method thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104825528B (en) | 2019-01-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104306959A (en) | Medicine composition for treating osteoporosis and preparation method thereof | |
| CN102526326B (en) | Traditional Chinese medicine for treating postmenopausal osteoporosis and preparation method thereof | |
| CN104825528B (en) | Have effects that increase the composition and its preparation method and application of bone density | |
| CN106110291A (en) | A kind of nutritional preparation for kidney cell reparation and preparation method thereof | |
| CN104922543A (en) | Medicine composite for treating amenorrhea and preparation method thereof | |
| CN109350653A (en) | The composition of improvement menopause symptom containing NMN, preparation and the preparation method and application thereof | |
| CN104547750A (en) | Traditional Chinese medicine composition for treating senile osteoporosis | |
| CN1207041C (en) | Medicine for treating osteoporosis and its preparing process | |
| CN103263601B (en) | Chinese herbal preparation for accelerating fracture post-operation healing and preparation method of Chinese herbal preparation | |
| CN101138597B (en) | Traditional Chinese medicine preparation for preventing and controlling osteoporosis and method of preparing the same | |
| CN102430076A (en) | Umbilici-applied weight-reducing powder | |
| CN104800720A (en) | Medicine for improving sexual desire of breeding bull and increasing yield of semen and preparation method of medicine | |
| CN102114070B (en) | Compound traditional Chinese medicine for treating osteoporosis and preparation method thereof | |
| CN106860656A (en) | It is a kind of to treat Chinese medicine composition of osteoporosis and its preparation method and application | |
| CN104524524B (en) | Medicine treating osteoporosis and preparing method | |
| CN106620133A (en) | Medicinal liquor having functions of invigorating the kidney, strengthening Yang and expelling wind-damp and preparation method thereof | |
| CN106511549A (en) | Traditional Chinese medicine composition containing millettia specisoa and preparation method of traditional Chinese medicine composition | |
| CN105853689A (en) | Application of green plum extract to preparation of health care product for treating osteoporosis | |
| CN101934046B (en) | Semifluid extract of deer foetus and preparation method thereof | |
| CN102068534B (en) | Chinese medicinal composition for relaxing tendons and activating collaterals and preparation method thereof | |
| CN102824565B (en) | Medicine for treating osteoporosis and preparation method thereof | |
| CN101732648B (en) | Medicament for treating nonunion | |
| CN101829221B (en) | American cockroach medicament composition for treating piles and anal fissure and preparation method thereof | |
| CN105796860A (en) | Quick-acting bone setting Yi medicine | |
| CN1250661A (en) | Medicinal composition for treating bone fracture and its preparing process |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| EXSB | Decision made by sipo to initiate substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190111 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |