CN104817347A - Preparation method of biological organic fertilizer for improving content of proteins in potato - Google Patents
Preparation method of biological organic fertilizer for improving content of proteins in potato Download PDFInfo
- Publication number
- CN104817347A CN104817347A CN201510141738.4A CN201510141738A CN104817347A CN 104817347 A CN104817347 A CN 104817347A CN 201510141738 A CN201510141738 A CN 201510141738A CN 104817347 A CN104817347 A CN 104817347A
- Authority
- CN
- China
- Prior art keywords
- sea
- tangle
- potato
- peru squid
- preparation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/20—Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses
Landscapes
- Fertilizers (AREA)
Abstract
The invention discloses a preparation method of a biological organic fertilizer for improving the content of proteins in potato. The method comprises the following steps: 1, preparing a peru squid enzymatic hydrolysis liquid; 2, preparing a sea-tangle enzymatic hydrolysis liquid; 3, mixing the peru squid enzymatic hydrolysis liquid with the sea-tangle enzymatic hydrolysis liquid in proportion, inoculating azotobacter, Bacillus licheniformis and an EM bacterium, fully mixing cow and sheep dung, corn straw and wheat straw, and fermenting; and 4, air-drying, crushing, and carrying out packaging preservation. The biological organic fertilizer prepared through respective enzymatic hydrolysis of peru squid and sea-tangle, inoculation of beneficial microbes, continuous addition of animal dung and crop straws and fermentation contains abundant amino acids and proteins, contains abundant and comprehensive nutrition, has a lasting fertilizer effect release time, accords with the growth demand of potato, makes planted potato have high content of proteins, and improves the economic benefit of plantation of the potato.
Description
Technical field:
The present invention relates to agriculture production fertilizer field, particularly relate to a kind of preparation method of biological organic fertilizer, be specifically related to a kind of preparation method that can improve the biological organic fertilizer of potato protein content.
Background technology:
Potato (SolanumtuberosumL.) has another name called potato, potato, potato etc., belongs to the annual herb plant of Solanaceae Solanum potato group.During the 17th century, potato imports China into.Yam starch content is high, can reach the 60%-80% of dry-matter, so a lot of area is regarded food crop to treat; Potato VITAMIN and rich in protein, a lot of area is regarded vegetables and is treated.Potato is food crop, is again vegetable crop, so more and more pay attention to the plantation of potato.
In ripening stage potato tuber, protein accounts for the 2-3% of fresh weight.Protein is one of important indicator evaluating Potato Quality quality, and the protein of potato and animal proteinum are very close, and quality is fine, and belong to complete protein, people's physical efficiency well absorbs.Comprise 19 seed amino acids in the protein of potato, total amount is 42.05%, and wherein the necessary amino acid wanted of needed by human body and non-essential amino acid respectively account for half.
Research shows, increase with the content of soluble protein in the increase potato tuber of amount of nitrogen, be 200kg/hm at amount of nitrogen thereupon
2time soluble proteins content the highest.Rational applied nitrogen can prevent stem senescence of leaves, increases the nutrient accumulation of stem tuber and the quantity of stem tuber, thus improves the output of potato and the content of stem tuber protein, the excessive (360kg/hm of applied nitrogen
2), or nitrogen top dressing stage improper (middle and later periods of breeding time), all can cause the excessive growth of plant, the ripening stage of stem tuber postpones, and susceptible disease, thus cause the reduction of Tuber yields and quality.With the continuous propelling of breeding time, the content of horse bell tomb stem tuber protein reduces gradually, and the content of different nitrogen form on potato tuber internal protein affects without significance.Use nitrogen phosphorus fertilizer and farm manure has increasing action to the content of protein in potato tuber, compared with not applying fertilizer, use the content that nitrogen phosphorus fertilizer and applications of farmyard manure all can increase potato tuber internal protein, and all with do not apply fertilizer in significant difference, nitrogen phosphorus fertilizer and farm manure compounding application make the content of protein on average increase by 1.27%, amplification reaches 12.41%, all reaches in pole significant difference, enrich the increase that farm manure contributes to patatin matter content as seen with each process.With the increase of amount of potassium applied, the content of potato tuber internal protein presents the trend first increasing and reduce afterwards, and K Amounts is at 180kg/hm
2time reach the highest, use too much potash fertilizer and the protein content in potato tuber can be made to reduce.The increase of the low potassium of high nitrogen to potato ball base protein has the effect of promotion, and this and nitrogen are that the bioelement that protein molecule synthesizes has main relation.When other factors is identical, application of zinc fertilizer can increase the content of patatin matter.
Chinese invention patent CN1744813 relates to breeding and the selection of potato, provide one and there is the allelic potato plants of at least one amf-or its gained part, described potato plants or part also provide the ability of the reserve protein of increase, and it is increased by the protein content of its stem tuber to characterize.And, the invention provides a kind of breeding and the method selecting to have the potato of the ability of the reserve protein of increase, comprise and will have at least one amf-allelic first parental generation potato and not have allelic second parent potato of amf-to hybridize, and selection one there is at least one amf-allelotrope and the protein content of its stem tuber higher than the filial generation of the protein content measured in described first parental generation or described second parental generation.This invention improves potato protein content by the mode changing gene, and make better products, the Potato Cultivars after gene alteration lacks permanently effective considering in security.
Chinese invention patent CN102134170A relates to the compound fertilizer dedicated and manufacture method of a kind of potato amino acid organic and inorganic, and it comprises the following each component of mass percent: the organic 22%-28% after urea 10%-13%, potassium sulfate 20%-23%, calcium superphosphate 22%-25%, harmless treatment, acidolysis aminoacids solution 16%-18%, wilkinite 3%-4%.Composite fertilizer in this invention can meet the psychological need of potato growth, both provided the nitrogen of proper ratio needed for potato growth, phosphorus, potassium, and utilized again amino acid to facilitate potato root system development, make it fully absorb nutrient, thus promote that potato tuber expands, improve potato yield; Adopt raw material containing the composition harmful to growth and development of plants, organic nutrient and inorganic nutrients assembly reasonable, add the nutrient slowly-released phase, greatly improve utilization rate of fertilizer, also improve Soil structure, be thus particularly suitable for the needs of potato single-dose application.Acidolysis aminoacids solution is added in this invention, improves amino acid and protein content in composite fertilizer, has certain influence after acidolysis for composite fertilizer pH, effectively gentle not as good as enzymatic hydrolysis condition.
Chinese invention patent CN103907459A discloses a kind of potato seed method for planting, and method is as follows: prepare before a, sowing; B, sowing; C, field management; D, results.The output of the new large level ground kind potato of implantation methods that this invention provides plantation is high, disease resistance strong, effectively solves and adopts traditional planting patterns plantation new large level ground kind potato to there is the not high and not high deficiency of P. infestans resistant of output.Comparatively traditional planting technology, the cultivation technique of this invention makes potato yield and quality all significantly increase, wherein output adds 15%, dry matter content adds 12.53%, reducing sugar content adds 20.00%, soluble protein content adds 6.76%, and starch content adds 19.07%, Vc content and adds 18.39%; Find first 3 days-5 days late blight of potato primary infection focus, adopt sterilant effectively can prevent and treat the late blight of potato, disease rate decreases 27.89%.This invention implantation methods is efficient, and more traditional planting patterns is more numerous and diverse.
Summary of the invention:
The present invention is directed to prior art Problems existing, a kind of preparation method that can improve the biological organic fertilizer of potato protein content is provided, the biological organic fertilizer that this preparation method prepares is comprehensively nutritious, fertilizer efficiency release is lasting, meet potato growth demand, in the potato that plantation obtains, protein content is high.
The invention provides following technical scheme:
A preparation method for the biological organic fertilizer of potato protein content can be improved, comprise the following steps:
The first step: prepare Peru squid enzymolysis solution;
Second step: preparation sea-tangle enzymolysis solution;
3rd step: the Peru squid enzymolysis solution prepared by the first step second step and sea-tangle enzymolysis solution are mixed in proportion rear kind of vinelandii, Bacillus licheniformis, EM bacterium, fully mix with cattle and sheep excrement, maize straw, wheat stalk after inoculation, regulate C/N ratio by urea and glucose, pass through H
2after O regulates moisture, content ferments;
4th step: rear air-dry pulverizing pack of having fermented is preserved.
Preferably, in the described the first step, the preparation process of Peru squid enzymolysis solution is:
The water adding 2-3 times of Peru squid weight after Peru squid cleaning is smashed to pieces and makes Peru squid slurry, after animal protease mixes with weight ratio 1:2 with Sumizyme MP, add in Peru squid slurry, addition is the 1.3-1.5% of Peru squid slurry quality, under 52-55 DEG C of water bath condition, individual hour of enzymolysis 7-8, obtains Peru squid enzymolysis solution after enzymolysis terminates.
Preferably, in described second step, the preparation process of sea-tangle enzymolysis solution is: smash into sea-tangle slurry to pieces by adding sea-tangle weight in wet base 8-10 water doubly after sea-tangle maceration, sea-tangle slurry pH is regulated to be the polygalacturonase adding kelp paste weight 0.5-0.7% after 4.5-4.7, enzymolysis 2-2.5 hour under 45-48 DEG C of bath temperature condition, it is 5.0-5.2 that enzymolysis terminates rear adjustment pH, obtains sea-tangle enzymolysis solution.
Preferably, in described 3rd step, Peru squid enzymolysis solution and the sea-tangle enzymolysis solution ratio by weight 1:4-5 is mixed.
Preferably, vinelandii, Bacillus licheniformis, EM bacterium in described 3rd step, inoculum size is respectively 5-7%, 3-5% and 7-12% of Peru squid enzymolysis solution and the rear liquid weight of sea-tangle enzymolysis mixing.
Preferably, in described 3rd step, after Peru squid enzymolysis solution and the combined inoculation of sea-tangle enzymolysis, mix after liquid weight 50-70 cattle and sheep excrement doubly, 28-40 maize straw doubly and 30-35 wheat stalk doubly after adding Peru squid enzymolysis solution and the mixing of sea-tangle enzymolysis.
Preferably, in described 3rd step, regulate C/N ratio to be 30-32:1, moisture content is 60-63%.Preferably, the fermentation time fermented in described 3rd step is 14-18 days.
Preferably, fermented in described 4th step rear air-dry be 15-18% to moisture content, pulverized 40 mesh sieves after air-dry, pack sealing preserve.
Preferably, in described 3rd step, vinelandii are azotobacter chroococcum, viable bacteria content >=3,000,000,000/gram, useful total count >=20,000,000,000 in described Bacillus licheniformis and EM bacterium/gram, addition is with living bacteria count gauge.
In recent decades, world economy sustainable growth, the requirement of people to agricultural is more and more higher, causes using inorganic fertilizer in a large number, on a large scale in agricultural planting.Because the fertilizer efficiency of this " new-type fertilizer " is rapid, commercially occupy huge share very soon, as organic fertilizer such as farm manures because its development effort power consumption is large, gather difficulty in a large number, the market share is atrophy gradually.The appearance of this phenomenon causes the use of fertilizer fewer and feweri, the usage quantity of mineral manure is increasing, finally a large amount of mineral manure applies farmland, the physics of soil, chemistry and biological property is caused to change, organic content reduces, the generation of the phenomenas such as soil microorganisms bacterial diversity is damaged, soil function reduction.
The feature of biological organic fertilizer: 1 improves chemical fertilizer utilization ratio, and biological organic fertilizer has the effects such as fixed nitrogen, phosphorus decomposing, potassium decomposing, greatly can improve the utilization ratio of chemical fertilizer.2 improvement soil, increase soil fertility, in biological organic fertilizer, not only organic component content enriches, in addition also containing be conducive to edatope improve probiotics.3 alleviate environmental pollution.4 improve crop quality, and the multiple nutritional components that biological organic fertilizer is rich in imparts the special fertilizer efficiency of fertilizer, proves after deliberation, and biological organic fertilizer can improve color and luster, the quality such as mouthfeel and growth vigor of crop.5 alleviate crop pests, and one is the bacteriostatic action of biological organic fertilizer self, and two is effect of biological organic fertilizer on crops growth, antibacterial, disease-resistant promoter action.
Peru squid is commonly called as jumbo flying squid (Dosidicus gigas), and its build is huge, and growth rapidly, life-span approximately one year, at 11-110cm not etc., some individuality is 200cm to carcass length, is one of squid kind that the individuality that up to the present finds is maximum, resource is enriched the most.Peru squid is mainly distributed in east, the Pacific Ocean, the most concentrated with Peru marine site, has highly migratory.Peru squid has the features such as look white is, nutritious, its edible portion is containing 15.6% crude protein, 1.5% crude fat and 1.0% ash content, a kind of high protein, low-fat acat, by Peru squid, especially its waste obtains the enzymolysis solution of high protein and aminoacids content by the mode of enzymolysis, adds in the fermenting process of farm manure and ferments together, have a good application prospect.
Beneficial effect of the present invention is:
1. the present invention adds Peru squid and sea-tangle, both are by containing rich in protein, amino acid and various mineral substance effective constituent after enzymolysis, again by mixing rear inoculation fermentation with farm organic fertilizer material, the biological organic fertilizer that fermentation obtains contains abundant comprehensive nutritive ingredient, especially useful amino acid and protein content very high, fertilizer efficiency release is lasting, meets potato growth demand, has significant effect for protein content in raising potato.
2. the present invention contains multiple effective microbe, retain the good function characteristic that general fertilizer has, can promote potato strain to nutritive element absorb and tangerine resists the pathogenic effects of some pathogenic micro-organism, alleviate the disease and pest in potato planting process, improve plantation quality and output, improve economy and zoology benifit.
3. Peru squid waste is applied to agricultural fertilizer fermentation arts by the present invention, has opened up Peru squid waste application new direction, has improve economic benefit, decrease environmental pollution.
Embodiment:
Be described in detail embodiments of the invention below, the present embodiment is implemented under premised on invention technical scheme, give detailed embodiment and concrete operating process, but protection scope of the present invention is not limited to following embodiment.The experimental program of unreceipted actual conditions in embodiment, the condition that conveniently condition or manufacturers advise usually is implemented.
Embodiment one
A preparation method for the biological organic fertilizer of potato protein content can be improved, comprise the following steps:
The first step: the water adding 2 times of squid weight after Peru squid cleaning is smashed to pieces and makes Peru squid slurry, after animal protease mixes with weight ratio 1:2 with Sumizyme MP, add in Peru squid slurry, addition is 1.3% of Peru squid slurry quality, enzymolysis 7 hours under 52 DEG C of water bath condition, obtains Peru squid enzymolysis solution after enzymolysis terminates;
Second step: smash into the water adding sea-tangle weight in wet base 8 times after sea-tangle maceration to pieces sea-tangle slurry, sea-tangle slurry pH is regulated to be the polygalacturonase adding kelp paste weight 0.5% after 4.5, enzymolysis 2 hours under 45-48 DEG C of bath temperature condition, it is 5.0 that enzymolysis terminates rear adjustment pH, obtains sea-tangle enzymolysis solution;
3rd step: the Peru squid enzymolysis solution prepared by the first step second step and sea-tangle enzymolysis solution plant vinelandii, Bacillus licheniformis, EM bacterium after the mixing of 1:4 ratio, inoculum size is respectively 5%, 3% and 7% of Peru squid enzymolysis solution and the rear liquid weight of sea-tangle enzymolysis mixing, mix after inoculation with after adding Peru squid enzymolysis solution and sea-tangle enzymolysis and mixing the wheat stalk of the cattle and sheep excrement of liquid weight 50 times afterwards, the maize straw of 28 times and 30 times, regulate C/N ratio to be 30:1 by urea and glucose, pass through H
2o regulates moisture content to be 60%, and ferment, fermentation time is 14 days;
4th step: fermented rear air-dry be 15% to moisture content, pulverized 40 mesh sieves after air-dry, pack sealing preserve.
Embodiment two
A preparation method for the biological organic fertilizer of potato protein content can be improved, comprise the following steps:
The first step: the water adding 3 times of squid weight after Peru squid cleaning is smashed to pieces and makes Peru squid slurry, after animal protease mixes with weight ratio 1:2 with Sumizyme MP, add in Peru squid slurry, addition is 1.5% of Peru squid slurry quality, enzymolysis 8 hours under 55 DEG C of water bath condition, obtains Peru squid enzymolysis solution after enzymolysis terminates;
Second step: smash into the water adding sea-tangle weight in wet base 10 times after sea-tangle maceration to pieces sea-tangle slurry, sea-tangle slurry pH is regulated to be the polygalacturonase adding kelp paste weight 0.7% after 4.7, enzymolysis 2.5 hours under 48 DEG C of bath temperature conditions, it is 5.2 that enzymolysis terminates rear adjustment pH, obtains sea-tangle enzymolysis solution;
3rd step: the Peru squid enzymolysis solution prepared by the first step second step and sea-tangle enzymolysis solution plant vinelandii, Bacillus licheniformis, EM bacterium after the mixing of 1:4-5 ratio, inoculum size is respectively 7%, 5% and 12% of Peru squid enzymolysis solution and the rear liquid weight of sea-tangle enzymolysis mixing, mix after inoculation with after adding Peru squid enzymolysis solution and sea-tangle enzymolysis and mixing the wheat stalk of the cattle and sheep excrement of liquid weight 70 times afterwards, the maize straw of 40 times and 35 times, regulate C/N ratio to be 32:1 by urea and glucose, pass through H
2o regulates moisture content to be 63%, and ferment, fermentation time is 18 days;
4th step: fermented rear air-dry be 18% to moisture content, pulverized 40 mesh sieves after air-dry, pack sealing preserve.
Embodiment three
A preparation method for the biological organic fertilizer of potato protein content can be improved, comprise the following steps:
The first step: the water adding 2 times of squid weight after Peru squid cleaning is smashed to pieces and makes Peru squid slurry, after animal protease mixes with weight ratio 1:2 with Sumizyme MP, add in Peru squid slurry, addition is 1.5% of Peru squid slurry quality, enzymolysis 8 hours under 52 DEG C of water bath condition, obtains Peru squid enzymolysis solution after enzymolysis terminates;
Second step: smash into the water adding sea-tangle weight in wet base 8 times after sea-tangle maceration to pieces sea-tangle slurry, sea-tangle slurry pH is regulated to be the polygalacturonase adding kelp paste weight 0.5% after 4.7, enzymolysis 2 hours under 48 DEG C of bath temperature conditions, it is 5.2 that enzymolysis terminates rear adjustment pH, obtains sea-tangle enzymolysis solution;
3rd step: the Peru squid enzymolysis solution prepared by the first step second step and sea-tangle enzymolysis solution plant vinelandii, Bacillus licheniformis, EM bacterium after the mixing of 1:4-5 ratio, inoculum size is respectively 5%, 5% and 7% of Peru squid enzymolysis solution and the rear liquid weight of sea-tangle enzymolysis mixing, mix after inoculation with after adding Peru squid enzymolysis solution and sea-tangle enzymolysis and mixing the wheat stalk of the cattle and sheep excrement of liquid weight 70 times afterwards, the maize straw of 28 times and 30 times, regulate C/N ratio to be 30:1 by urea and glucose, pass through H
2o regulates moisture content to be 63%, and ferment, fermentation time is 14 days;
4th step: fermented rear air-dry be 15% to moisture content, pulverized 40 mesh sieves after air-dry, pack sealing preserve.
Embodiment four
A preparation method for the biological organic fertilizer of potato protein content can be improved, comprise the following steps:
The first step: the water adding 2 times of squid weight after Peru squid cleaning is smashed to pieces and makes Peru squid slurry, after animal protease mixes with weight ratio 1:2 with Sumizyme MP, add in Peru squid slurry, addition is 1.4% of Peru squid slurry quality, enzymolysis 7 hours under 53 DEG C of water bath condition, obtains Peru squid enzymolysis solution after enzymolysis terminates;
Second step: smash into the water adding sea-tangle weight in wet base 9 times after sea-tangle maceration to pieces sea-tangle slurry, sea-tangle slurry pH is regulated to be the polygalacturonase adding kelp paste weight 0.6% after 4.6, enzymolysis 2.4 hours under 47 DEG C of bath temperature conditions, it is 5.1 that enzymolysis terminates rear adjustment pH, obtains sea-tangle enzymolysis solution;
3rd step: the Peru squid enzymolysis solution prepared by the first step second step and sea-tangle enzymolysis solution plant vinelandii, Bacillus licheniformis, EM bacterium after the mixing of 1:4-5 ratio, inoculum size is respectively 6%, 4% and 10% of Peru squid enzymolysis solution and the rear liquid weight of sea-tangle enzymolysis mixing, mix after inoculation with after adding Peru squid enzymolysis solution and sea-tangle enzymolysis and mixing the wheat stalk of the cattle and sheep excrement of liquid weight 60 times afterwards, the maize straw of 30 times and 33 times, regulate C/N ratio to be 31:1 by urea and glucose, pass through H
2o regulates moisture content to be 62%, and ferment, fermentation time is 16 days;
4th step: fermented rear air-dry be 17% to moisture content, pulverized 40 mesh sieves after air-dry, pack sealing preserve.
Vinelandii described above are azotobacter chroococcum, and purchased from Yangzhou Haicheng Bioisystech Co., Ltd, product net weight specification is 25KG/ bag, 24 months quality guaranteed perioves, Powdered, viable bacteria content >=3,000,000,000/gram;
Described Bacillus licheniformis is purchased from Bai Fude bio tech ltd, Wuxi, product specification is 25 kilograms/barrel, validity period 2 years, Powdered, containing useful total count >=50,000,000,000/gram, addition is in probiotics sum, so in above-mentioned 3rd step, inoculum size 3% is converted into said preparation addition is 1.2% this bacterium powder;
Described EM bacterium is purchased from Cangzhou City Yi Hong animal health-care product company limited, and product specification is 25kg/ bucket, grey powder, total viable count >=20,000,000,000/gram.
Planting experiment one
Experiment Xiangyang, Hubei, place;
Supply examination soil preceding crop to be cotton, the sill nutrient situation of soil is: pH 4.46, organic 10.06g/kg, alkali-hydrolyzable nitrogen 103.02mg/kg, rapid available phosphorus 39.44mg/kg, available potassium 98.09mg/kg, full nitrogen 0.77g/kg, full phosphorus 0.62g/kg, full potassium 9.76g/kg.
18 fritters are divided into for examination soil, every three pieces divide one group at random, be divided into six groups, use the potato bio-organic fertilizer special that preparation method described in embodiment one to four prepares respectively, be denoted as treatment group one to four group, amount of application is 70kg/ mu, uses the potato bio-feritlizer that market is bought for remaining two pieces, and amount of application is used according to purchase bio-feritlizer specification sheets.
Varieties of plant is Potato Cultivars early-spring herbs, and planting density is 84000 caves/hm
2, every cave 1 strain, general planting mode and way to manage, plant latter six months receipts.
Statistics gathers in the crops protein content in rear potato yield and the fresh sample of ripening stage potato tuber, and protein content Coomassie brilliant G-250 method measures, and statistics is in table one.
Table one: protein content in planting experiment one potato yield potato tuber
| Planting experiment one | Output (thousand grams/acre) | Protein content (%) |
| Treatment group one | 1346.4±164.2 | 6.53±0.85 |
| Treatment group two | 1420.5±201.4 | 6.85±0.59 |
| Treatment group three | 1308.9±187.5 | 6.28±1.02 |
| Treatment group four | 1298.7±100.5 | 6.37±0.94 |
| Control group one | 1216.5±143.7 | 3.18±0.86 |
| Control group two | 1204.5±147.9 | 4.15±1.05 |
Experimental data adopts the phraseology of mean number ± variance.
Planting experiment two
Experiment place Ya'an Sichuan province.
Supply examination soil to be husky earth rice soil, fertility is even, middle fertility, and irrigation and drainage are convenient, and the main physicochemical property of soil is alkali-hydrolyzable nitrogen 143.50mg/kg, rapid available phosphorus 50.90mg/kg, available potassium 22.80mg/kg, and organic 23.80g/kg, PH are 5.80.
For examination soil random division 18 fritters, every three pieces divide one group at random, be divided into six groups, use the potato bio-organic fertilizer special that preparation method described in embodiment one to four prepares respectively, be denoted as treatment group one to four group, amount of application is 40kg/ mu, uses the potato bio-feritlizer that market is bought for remaining two pieces, and amount of application is used according to purchase bio-feritlizer specification sheets.
Supply examination Potato Cultivars to be river taro 117, planting density is 5800 caves/hm2, every cave 1 strain, general planting mode and way to manage, plants latter 5 months receipts.
Statistics gathers in the crops protein content in rear potato yield and the fresh sample of ripening stage potato tuber, and use Kjeldahl nitrogen determination gross protein value, statistics is in table two.
Table two: protein content in planting experiment two potato yield potato tuber
| Planting experiment two | Output (thousand grams/acre) | Gross protein value (%) |
| Treatment group one | 1872.98±204.62 | 6.25±1.02 |
| Treatment group two | 1905.61±109.54 | 5.21±1.03 |
| Treatment group three | 1854.67±231.76 | 4.98±1.24 |
| Treatment group four | 1826.93±209.38 | 5.69±1.17 |
| Control group one | 1135.62±199.80 | 1.08±2.04 |
| Control group two | 1100.56±204.62 | 1.31±3.81 |
Experimental data adopts the phraseology of mean number ± variance.
By above-mentioned experimental data, we can find out, use the biological organic fertilizer that the inventive method prepares and effectively can improve potato yield and potato ball caulome internal protein content.
Above content is only better embodiment of the present invention, and for those of ordinary skill in the art, according to thought of the present invention, all will change in specific embodiments and applications, this description should not be construed as limitation of the present invention.
Claims (10)
1. can improve a preparation method for the biological organic fertilizer of potato protein content, it is characterized in that, comprise the following steps:
The first step: prepare Peru squid enzymolysis solution;
Second step: preparation sea-tangle enzymolysis solution;
3rd step: the Peru squid enzymolysis solution prepared by the first step second step and sea-tangle enzymolysis solution are mixed in proportion rear kind of vinelandii, Bacillus licheniformis, EM bacterium, fully mix with cattle and sheep excrement, maize straw, wheat stalk after inoculation, regulate C/N ratio by urea and glucose, pass through H
2after O regulates moisture, content ferments;
4th step: rear air-dry pulverizing pack of having fermented is preserved.
2. a kind of preparation method that can improve the biological organic fertilizer of potato protein content according to claim 1, it is characterized in that: in the described the first step, the preparation process of Peru squid enzymolysis solution is: the water adding 2-3 times of Peru squid weight after Peru squid cleaning is smashed to pieces and makes Peru squid slurry, after animal protease mixes with weight ratio 1:2 with Sumizyme MP, add in Peru squid slurry, addition is the 1.3-1.5% of Peru squid slurry quality, individual hour of enzymolysis 7-8 under 52-55 DEG C of water bath condition, Peru squid enzymolysis solution is obtained after enzymolysis terminates.
3. a kind of preparation method that can improve the biological organic fertilizer of potato protein content according to claim 1, it is characterized in that: in described second step, the preparation process of sea-tangle enzymolysis solution is: smash into sea-tangle slurry to pieces by adding sea-tangle weight in wet base 8-10 water doubly after sea-tangle maceration, sea-tangle slurry pH is regulated to be the polygalacturonase adding kelp paste weight 0.5-0.7% after 4.5-4.7, enzymolysis 2-2.5 hour under 45-48 DEG C of bath temperature condition, it is 5.0-5.2 that enzymolysis terminates rear adjustment pH, obtains sea-tangle enzymolysis solution.
4. a kind of preparation method that can improve the biological organic fertilizer of potato protein content according to claim 1, is characterized in that: Peru squid enzymolysis solution and the sea-tangle enzymolysis solution ratio by weight 1:4-5 mixed in described 3rd step.
5. a kind of preparation method that can improve the biological organic fertilizer of potato protein content according to claim 1, it is characterized in that: vinelandii, Bacillus licheniformis, EM bacterium in described 3rd step, inoculum size is respectively 5-7%, 3-5% and 7-12% of Peru squid enzymolysis solution and the rear liquid weight of sea-tangle enzymolysis mixing.
6. a kind of preparation method that can improve the biological organic fertilizer of potato protein content according to claim 1, it is characterized in that: in described 3rd step, after Peru squid enzymolysis solution and the combined inoculation of sea-tangle enzymolysis, mix after liquid weight 50-70 cattle and sheep excrement doubly, 28-40 maize straw doubly and 30-35 wheat stalk doubly after adding Peru squid enzymolysis solution and the mixing of sea-tangle enzymolysis.
7. a kind of preparation method that can improve the biological organic fertilizer of potato protein content according to claim 1, is characterized in that: in described 3rd step, and regulate C/N ratio to be 30-32:1, moisture content is 60-63%.
8. a kind of preparation method that can improve the biological organic fertilizer of potato protein content according to claim 1, is characterized in that: the fermentation time fermented in described 3rd step is 14-18 days.
9. a kind of preparation method that can improve the biological organic fertilizer of potato protein content according to claim 1, it is characterized in that: fermented in described 4th step rear air-dry to moisture content be 15-18%, pulverized 40 mesh sieves after air-dry, pack sealing is preserved.
10. a kind of preparation method that can improve the biological organic fertilizer of potato protein content according to claim 5, it is characterized in that: in described 3rd step, vinelandii are azotobacter chroococcum, viable bacteria content >=3,000,000,000/gram, useful total count >=20,000,000,000 in described Bacillus licheniformis and EM bacterium/gram, addition is with living bacteria count gauge.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510141738.4A CN104817347A (en) | 2015-03-30 | 2015-03-30 | Preparation method of biological organic fertilizer for improving content of proteins in potato |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510141738.4A CN104817347A (en) | 2015-03-30 | 2015-03-30 | Preparation method of biological organic fertilizer for improving content of proteins in potato |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104817347A true CN104817347A (en) | 2015-08-05 |
Family
ID=53727857
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510141738.4A Pending CN104817347A (en) | 2015-03-30 | 2015-03-30 | Preparation method of biological organic fertilizer for improving content of proteins in potato |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104817347A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105837270A (en) * | 2016-03-29 | 2016-08-10 | 四川大学 | Method for preparing organic fertilizer from crop straws |
| CN105985174A (en) * | 2015-12-29 | 2016-10-05 | 芜湖润蓝生物科技有限公司 | Efficient fertilizer with modified animal protein for potato nutrition improvement and preparation method of efficient fertilizer |
| WO2021135911A1 (en) * | 2019-12-31 | 2021-07-08 | 广西神冠胶原生物集团有限公司 | Method for enzymatically producing high-activity biological organic fertilizer |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1709836A (en) * | 2005-06-09 | 2005-12-21 | 中国水产科学研究院南海水产研究所 | Organic biologic fertilizer and its preparing method |
| CN102174428A (en) * | 2010-12-28 | 2011-09-07 | 南京农业大学 | Antagonistic bacteria for preventing bacterial wilt of potatoes and microorganism organic fertilizer thereof |
| CN103724126A (en) * | 2013-12-15 | 2014-04-16 | 张彩年 | Multielement high-efficiency active organic liquid fertilizer and preparation method thereof |
| CN103880482A (en) * | 2014-03-21 | 2014-06-25 | 国农生物肥料连云港有限责任公司 | Preparation method of crop organic fertilizer |
| CN104177136A (en) * | 2013-05-21 | 2014-12-03 | 徐波勇 | Method for extracting active substances for fertilizers from algae |
-
2015
- 2015-03-30 CN CN201510141738.4A patent/CN104817347A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1709836A (en) * | 2005-06-09 | 2005-12-21 | 中国水产科学研究院南海水产研究所 | Organic biologic fertilizer and its preparing method |
| CN102174428A (en) * | 2010-12-28 | 2011-09-07 | 南京农业大学 | Antagonistic bacteria for preventing bacterial wilt of potatoes and microorganism organic fertilizer thereof |
| CN104177136A (en) * | 2013-05-21 | 2014-12-03 | 徐波勇 | Method for extracting active substances for fertilizers from algae |
| CN103724126A (en) * | 2013-12-15 | 2014-04-16 | 张彩年 | Multielement high-efficiency active organic liquid fertilizer and preparation method thereof |
| CN103880482A (en) * | 2014-03-21 | 2014-06-25 | 国农生物肥料连云港有限责任公司 | Preparation method of crop organic fertilizer |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105985174A (en) * | 2015-12-29 | 2016-10-05 | 芜湖润蓝生物科技有限公司 | Efficient fertilizer with modified animal protein for potato nutrition improvement and preparation method of efficient fertilizer |
| CN105837270A (en) * | 2016-03-29 | 2016-08-10 | 四川大学 | Method for preparing organic fertilizer from crop straws |
| WO2021135911A1 (en) * | 2019-12-31 | 2021-07-08 | 广西神冠胶原生物集团有限公司 | Method for enzymatically producing high-activity biological organic fertilizer |
| CN114901615A (en) * | 2019-12-31 | 2022-08-12 | 广西神冠胶原生物集团有限公司 | Method for enzymatic production of high-activity bio-organic fertilizer |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101637101B (en) | Industrial cultivation method of selenium-enriched agaricus bisporus | |
| CN104529573B (en) | Many effect ferment fertilizer that a kind of agricultural wastes make | |
| CN106518284A (en) | Bio-organic fertilizer for dragon fruit planting and preparing method thereof | |
| CN104177139A (en) | Chicken manure organic fertilizer for microbial fermentation | |
| CN105379560A (en) | Method for cultivating edible fungi | |
| CN103274834B (en) | Special insecticidal sterilizing microbial fertilizer for garlic and preparation method thereof | |
| CN106688849A (en) | Soilless culture method of Chinese flowering cabbage | |
| CN103733874A (en) | Wildmimic cultivation method of ganoderma lucidum | |
| CN1398824A (en) | Bioorganic fertilizer and its production process | |
| CN105036921A (en) | Plant nutrient solution and preparing method thereof | |
| CN103880487A (en) | Three-in-one green manure fermentation nutritional agent and application method thereof | |
| CN105237147A (en) | Culture medium for oyster mushroom and preparation method thereof | |
| CN104788237B (en) | A kind of wheat selenium-rich zinc-iron microbial fermentation fertilizer and its preparation method and application | |
| CN107226747A (en) | A kind of macaque special bio-organic fertilizer for peaches prepared by primary raw material of underground residuum | |
| CN105110944A (en) | Agaricus bisporus culture substrate and preparation method thereof | |
| CN109748607A (en) | A kind of dedicated Selenium-rich nutrient solution of greenhouse crops and preparation method thereof | |
| CN105110945A (en) | Enoki mushroom culture substrate and preparation method thereof | |
| CN109020727A (en) | A kind of bio-organic fertilizer and preparation method thereof preventing Asparagus Stem Blight | |
| CN107556125A (en) | A kind of ecological organic fertilizer for vegetable cultivation and preparation method thereof | |
| CN105009903A (en) | Pollution-free high-yield cultivation method for watermelon | |
| CN103992192A (en) | Microbial compound fertilizer using fermented Zizania aquatica leaves as matrix and preparation method thereof | |
| CN103387431B (en) | Preparation method of pre-fermented chicken manure for agaricus bisporus culture medium | |
| CN104817347A (en) | Preparation method of biological organic fertilizer for improving content of proteins in potato | |
| CN109601479A (en) | A kind of stereo circulating production method of selenium-rich live pig meat | |
| CN107125017A (en) | A kind of breeding method of mushroom |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| EXSB | Decision made by sipo to initiate substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20150805 |