CN104177136A - Method for extracting active substances for fertilizers from algae - Google Patents
Method for extracting active substances for fertilizers from algae Download PDFInfo
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- CN104177136A CN104177136A CN201310188701.8A CN201310188701A CN104177136A CN 104177136 A CN104177136 A CN 104177136A CN 201310188701 A CN201310188701 A CN 201310188701A CN 104177136 A CN104177136 A CN 104177136A
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Abstract
The invention relates to a method for extracting active substances for fertilizers from algae, and belongs to the biotechnological field. Algal polysaccharides, alginic acid, chelated trace elements and other effective components are extracted from dry alga powder through homogenizing, alcohol extraction, water extraction, cold alkali liquid extraction, cellulose-protein compound enzyme hydrolysis and ultrafilter membrane separation technologies, and lay a foundation for the production of algal biological fertilizers. The method is mainly characterized by the application of the compound enzyme and the determination of enzymatic hydrolysis conditions. Cellulase and protease are selected by the inventor to carry out a biochemical reaction in a stage manner. Polysaccharides, alginic acid and other substances in alga cells are precipitated in the invention, and the activities of the above substances are maintained. Enzymatic hydrolyzed extract products undergo ultrafilter membrane separation to remove substances with the molecular weight of above 8,000,000 in order to make nutrition components concentrated and the active substances easily absorbed by plants. The extraction method using the compound enzyme hydrolysis extraction and ultrafilter membrane separation technologies to optimize the algal active components is initiated by the inventor.
Description
Technical field:
A method of extracting fertilizer active substance from marine alga, belongs to biotechnology field.Produce in order to extract the activeconstituents that marine alga fertilizer is used from marine alga.We use cellulase and proteolytic enzyme, adopt biochemical reaction abstraction technique, decompose alginic cell wall, extract the effective constituents such as Sargassum polysaccharides, Lalgine, chelated microelements, and isolate molecular weight by micron order membrane separation technique and exceed 8,000,000 supra polymer material, fertilizer nutritive element is more easily absorbed by plants.Prozyme technology can ensure biochemical reaction mild condition, can avoid the decomposition of polysaccharide, chelating element, guarantees the activity of active substance.
Background technology:
In marine alga, can utilize nutritive ingredient to have kind more than 60, wherein in dry-matter, Sargassum polysaccharides, Lalgine, chelated microelement, amino acid etc. account for the 20%-40% of dry substance mixture, the materials such as Sargassum polysaccharides are contained in alginic cell wall, as direct use can not be absorbed by plants, do not have the nutritive validity of fertilizer.Want to make full use of the nutritive substance in marine alga, must carry out exquisiteness to marine alga and extract.
Seaweed bio activeconstituents extraction process complexity, is difficult for grasping.Marine alga active substance extraction method mainly contains chemical leaching test, physics extraction method and biological enzyme extraction method.Chemical leaching test technique is simple, mainly to heat with strong acid and strong base, make alginic cell broken wall, extract domestic seaweed fertilizer manufacturing enterprise now and adopt this kind of method more, but while extracting active substance by the method, strong acid or highly basic destroy larger to polysaccharide, chelating state element, and the Lalgine extracting easily produces precipitation, affect Fertilizer application effect.Physics extraction method is mainly ultrasonic extraction and super critical extraction, and the method equipment requirements is high, and technical sophistication is only suitable for now in laboratory study, is unsuitable for business research and popularization.
The inventor utilizes complex enzyme hydrolysis abstraction technique and ultra-filtration membrane isolation technique, has optimized the extraction process of marine alga activeconstituents, has improved nutrient content and the activity of Seaweed Extract, has improved quality product.
Summary of the invention:
The techniques such as seaweed meal (being mainly the algae dry-matteies such as Enteromorpha, fragrant plant mentioned in ancient texts, sargassun, sea-tangle) is homogeneous through supper micron mill shearing, alcohol extracting, water extraction, the extraction of cold alkali lye, Mierocrystalline cellulose-albumen complex enzyme zymohydrolysis, ultra-filtration membrane separation, can extract the effective constituents such as Sargassum polysaccharides, Lalgine, chelated microelements.The method has effectively been extracted and has been produced the active substance that fertilizer is used, and then lays the foundation for producing the water-soluble handle of efficient seaweed bio.
Gordian technique of the present invention is the application of prozyme, and enzymatic hydrolysis condition is definite.The inventor selects cellulase and proteolytic enzyme segmentation to carry out biochemical reaction, has explored the add-on of enzyme between 0.1%-1.5%, and hydrolysis temperature is controlled at 45 DEG C-65 DEG C, pH value at 4.5-5.5; The first step enzymolysis only adds Mierocrystalline cellulose, promote the just one-step hydrolysis of cell walls of marine alga, second step, adds proteolytic enzyme and a small amount of cellulase, main with being hydrolyzed membranin by marine alga membrane structure destruction, thus polyose, Lalgine, chelated microelement material in alginic cell are separated out.
Extract after enzymolysis, by micron order ultra-filtration membrane isolation technique, isolates molecular weight and exceedes 8,000,000 supra polymer material, and polysaccharide, Lalgine, chelated microelement are concentrated, and the active substance extracting is more easily absorbed by plants.
Brief description of the drawings:
Fig. 1 extracts fertilizer active substance process flow sheet from marine alga.
Embodiment:
Choose natural free of contamination marine alga (being mainly the algae dry-matteies such as Enteromorpha, fragrant plant mentioned in ancient texts, sargassun, sea-tangle), after removal of impurities airing, grind to form dry powder, be beneficial to preserve.When extraction, dry powder is dipped in to pH to be approached in neutral water, after supper micron mill shearing is homogeneous, through steps such as alcohol extracting, water extraction, the extraction of cold alkali lye, Mierocrystalline cellulose-albumen complex enzyme zymohydrolysis, ultra-filtration membrane separation, can extract the effective constituents such as Sargassum polysaccharides, Lalgine, chelated microelements, can be used to process marine alga fertilizer.This invention reaction conditions gentleness, has avoided the hot strong acid-base destruction to seaweed nutritive material.
From marine alga, extract fertilizer active substance embodiment mono-:
The first step: after seaweed meal and pH approach neutral water and mix well in the ratio of 1: 20 to 1: 10, enter supper micron mill and shear homogeneous.
Second step: the sample after homogeneous is through reduced-pressure backflow, and temperature is controlled at 50 DEG C-70 DEG C, carries out water extraction; After water extraction, concentrated extracting solution is to below 2096 of original volume.Primary extract is placed in the alkaline solution of 40 times 11%, extracts 10 hours within the scope of 2-6 DEG C, is adjusted to pH value approximately 7.0 with acetic acid, has extracted rear centrifugally, collects filtrate, contains the Sargassum polysaccharides that molecular weight is little in filtrate, can obtain active substance through underpressure distillation desalination.Filter cake enters next step processing.
The 3rd step: after filter cake 1: 10 is homogeneous in water, drops into biochemical reaction tank and carry out enzyme digestion reaction.The present invention selects cellulase and proteolytic enzyme segmentation to carry out biochemical reaction, and the add-on of enzyme is between 0.1%-1.5%, and hydrolysis temperature is controlled at 45 DEG C-65 DEG C, pH value at 4.5-5.5.First enzymolysis only adds and accounts for filter cake total amount 0.4% cellulase, promote the just one-step hydrolysis of cell walls of marine alga, react after 4 hours, add again the proteolytic enzyme that accounts for filter cake total amount 0.3%, utilize with being hydrolyzed membranin marine alga membrane structure is destroyed, polyose, Lalgine, chelated microelement material in alginic cell are separated out.
The 4th step: the extract after enzymolysis, by ultra-filtration membrane isolation technique, segregation excision molecular weight exceedes 6,000,000 supra polymer material, polysaccharide, Lalgine, chelated microelement are concentrated, concentrated solution is through decompression Air drying, can obtain the fertilizer active substances such as Sargassum polysaccharides, Lalgine, chelated microelements, active substance total recovery can reach 20.7%.
From marine alga, extract fertilizer active substance embodiment bis-:
The first step: after seaweed meal and pH approach neutral water and mix well in the ratio of 1: 20 to 1: 10, enter supper micron mill and shear homogeneous.
Second step: the sample after homogeneous is through concentrating under reduced pressure, it is original 10% that volume is condensed to, and adds the ethanol of 9 times of volumes, refluxes 3 hours, filters, and collects filtrate, and filtrate pressurization is concentrated into paste, obtains green alga primary extract.Primary extract is placed in the alkaline solution of 40 times 11%, extracts 10 hours within the scope of 2-6 DEG C, is adjusted to pH value approximately 7.0 with acetic acid, has extracted rear centrifugally, collects filtrate, contains the Sargassum polysaccharides that molecular weight is little in filtrate, can obtain active substance through underpressure distillation desalination.Filter cake enters next step processing.
The 3rd step: after filter cake 1: 10 is homogeneous in water, drops into biochemical reaction tank and carry out enzyme digestion reaction.The add-on of enzyme is between 0.1%-1.5%, and hydrolysis temperature is controlled at 45 DEG C-65 DEG C, pH value at 4.5-5.5.First add and account for filter cake total amount 0.3% cellulase, promote the just one-step hydrolysis of cell walls of marine alga, react after 4 hours, add the proteolytic enzyme and 0.1% cellulase that account for filter cake total amount 0.3%, utilize with being hydrolyzed membranin marine alga membrane structure is destroyed, polyose, Lalgine, chelated microelement material in alginic cell are separated out.
The 4th step: the extract after enzymolysis, by ultra-filtration membrane isolation technique, segregation excision molecular weight exceedes 6,000,000 supra polymer material, polysaccharide, Lalgine, chelated microelement are concentrated, concentrated solution, through decompression Air drying, can obtain the fertilizer active substances such as Sargassum polysaccharides, Lalgine, chelated microelements.Active substance total recovery can reach 23.8%.
Claims (3)
1. one kind is extracted the method for fertilizer active substance from marine alga.
The method comprises: the techniques such as seaweed meal (being mainly the algae dry-matteies such as Enteromorpha, fragrant plant mentioned in ancient texts, sargassun, sea-tangle) is homogeneous through supper micron mill shearing, alcohol extracting, water extraction, the extraction of cold alkali lye, Mierocrystalline cellulose-albumen complex enzyme zymohydrolysis, ultra-filtration membrane separation, extract the effective constituents such as Sargassum polysaccharides, Lalgine, chelated microelements.
Gordian technique of the present invention is the application of prozyme, and enzymatic hydrolysis condition is definite.The inventor selects cellulase and proteolytic enzyme segmentation to carry out biochemical reaction, has explored the add-on of enzyme between 0.1%-1.59%, and hydrolysis temperature is controlled at 45 DEG C-65 DEG C, pH value at 4.5-5.5; The first step enzymolysis only adds Mierocrystalline cellulose, promote the just one-step hydrolysis of cell walls of marine alga, second step, adds proteolytic enzyme and a small amount of cellulase, main with being hydrolyzed membranin by marine alga membrane structure destruction, thus polyose, Lalgine, chelated microelement material in alginic cell are separated out.
2. produce marine alga active substance according to claim 1 processing method, can obtain the various active materials such as Sargassum polysaccharides, Lalgine, chelated microelements.
3. the various active materials such as the Sargassum polysaccharides, Lalgine, chelated microelements extracting by the inventive method, can be used as marine alga fertilizer and produce.
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Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104529570A (en) * | 2014-12-10 | 2015-04-22 | 济南航晨生物科技有限公司 | Novel method for extracting seaweed intermediate by enzyme process |
| CN104817347A (en) * | 2015-03-30 | 2015-08-05 | 苏州仁成生物科技有限公司 | Preparation method of biological organic fertilizer for improving content of proteins in potato |
| CN106008094A (en) * | 2016-05-23 | 2016-10-12 | 青岛海大生物集团有限公司 | Seaweed potassium fertilizer and preparation method thereof |
| CN106923337A (en) * | 2017-03-15 | 2017-07-07 | 福建农林大学 | A kind of preparation method and applications of spirulina full agonist |
| CN106942739A (en) * | 2017-03-15 | 2017-07-14 | 福建农林大学 | A kind of preparation method of marine alga full agonist |
| CN109020666A (en) * | 2018-07-18 | 2018-12-18 | 威海市世代海洋生物科技股份有限公司 | Kelp digests liquid fertilizer and preparation method thereof, application method |
| CN112167268A (en) * | 2019-07-02 | 2021-01-05 | 青岛苏贝尔作物营养有限公司 | Method for producing agricultural seaweed extract by physical and biological combination method and seaweed extract |
| CN112426381A (en) * | 2021-01-15 | 2021-03-02 | 晟丰(烟台)农业科技有限公司 | Marine biological active cosmetic |
| CN112451429A (en) * | 2021-01-26 | 2021-03-09 | 晟丰(烟台)农业科技有限公司 | Marine biological active cosmetic |
| CN113896590A (en) * | 2021-09-18 | 2022-01-07 | 青岛蓝宝海洋生物科技有限公司 | Enzymolysis processing method of seaweed extract for fertilizer |
| CN114164240A (en) * | 2021-09-08 | 2022-03-11 | 新洋丰农业科技股份有限公司 | Preparation method of seaweed extract by segmented enzymolysis |
| CN115590894A (en) * | 2022-10-24 | 2023-01-13 | 厦门一三九生物科技有限公司（Cn） | Preparation method of seaweed full-active substance |
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Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104529570A (en) * | 2014-12-10 | 2015-04-22 | 济南航晨生物科技有限公司 | Novel method for extracting seaweed intermediate by enzyme process |
| CN104817347A (en) * | 2015-03-30 | 2015-08-05 | 苏州仁成生物科技有限公司 | Preparation method of biological organic fertilizer for improving content of proteins in potato |
| CN106008094A (en) * | 2016-05-23 | 2016-10-12 | 青岛海大生物集团有限公司 | Seaweed potassium fertilizer and preparation method thereof |
| CN106923337A (en) * | 2017-03-15 | 2017-07-07 | 福建农林大学 | A kind of preparation method and applications of spirulina full agonist |
| CN106942739A (en) * | 2017-03-15 | 2017-07-14 | 福建农林大学 | A kind of preparation method of marine alga full agonist |
| CN109020666A (en) * | 2018-07-18 | 2018-12-18 | 威海市世代海洋生物科技股份有限公司 | Kelp digests liquid fertilizer and preparation method thereof, application method |
| CN112167268A (en) * | 2019-07-02 | 2021-01-05 | 青岛苏贝尔作物营养有限公司 | Method for producing agricultural seaweed extract by physical and biological combination method and seaweed extract |
| CN112167268B (en) * | 2019-07-02 | 2021-12-10 | 青岛苏贝尔作物营养有限公司 | Method for producing agricultural seaweed extract by physical and biological combination method and seaweed extract |
| CN112426381A (en) * | 2021-01-15 | 2021-03-02 | 晟丰(烟台)农业科技有限公司 | Marine biological active cosmetic |
| CN112451429A (en) * | 2021-01-26 | 2021-03-09 | 晟丰(烟台)农业科技有限公司 | Marine biological active cosmetic |
| CN114164240A (en) * | 2021-09-08 | 2022-03-11 | 新洋丰农业科技股份有限公司 | Preparation method of seaweed extract by segmented enzymolysis |
| CN114164240B (en) * | 2021-09-08 | 2022-11-25 | 新洋丰农业科技股份有限公司 | Preparation method of seaweed extract by segmented enzymolysis |
| CN113896590A (en) * | 2021-09-18 | 2022-01-07 | 青岛蓝宝海洋生物科技有限公司 | Enzymolysis processing method of seaweed extract for fertilizer |
| CN115590894A (en) * | 2022-10-24 | 2023-01-13 | 厦门一三九生物科技有限公司（Cn） | Preparation method of seaweed full-active substance |
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