CN112451429A - Marine biological active cosmetic - Google Patents
Marine biological active cosmetic Download PDFInfo
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- CN112451429A CN112451429A CN202110106135.6A CN202110106135A CN112451429A CN 112451429 A CN112451429 A CN 112451429A CN 202110106135 A CN202110106135 A CN 202110106135A CN 112451429 A CN112451429 A CN 112451429A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9706—Algae
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
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Abstract
The invention relates to a marine biological activity cosmetic which is characterized by containing seaweed essence, wherein the seaweed essence is prepared according to the following steps: the seaweed extract is a product obtained by carrying out enzymolysis on seaweed, wherein the enzymolysis sequentially comprises cellulase enzymolysis, saccharification enzymolysis and protease enzymolysis. The application reduces the damage to the seaweed and reserves more seaweed active substances. The application has the effect of delaying senility.
Description
Technical Field
The invention relates to a marine biological activity cosmetic.
Background
Since marine organisms live in severe environments with high humidity, high salt content and high cold, the marine organisms need to have strong repairing and water-retaining functions in order to adapt to the environment, so that the marine organisms have a good prospect in cosmetics. The seaweed has the advantages of low cost and easy obtainment, so the seaweed is widely applied to cosmetics, but the extraction method directly influences the use effect of the cosmetics.
At present, the seaweed is extracted by adopting a chemical method, the damage to the effective ingredients of the seaweed is great, the prepared seaweed essence cannot be satisfied, and the enzyme has specificity, so the damage to the effective ingredients of the seaweed can be reduced by adopting the enzyme extraction; the complex enzyme is adopted for extraction, and the problem of incomplete extraction exists during extraction because the activity of the enzyme is greatly influenced by temperature and pH. The combined extraction with enzymes is influenced by the selection of enzymes, temperature, pH and even the order of addition of enzymes will influence the extraction product.
At present, the technology of extracting the seaweed by enzyme combination also exists, for example, the patent number CN 106834358A adopts the steps of firstly carrying out enzymolysis by cellulase and beta-glucosidase at 40-80 ℃, then carrying out solid-liquid separation, and collecting supernatant; adjusting pH to 5-6, adding liquefying enzyme into the supernatant, performing enzymolysis in water bath at 50-70 deg.C for 1-3 days, adjusting pH to 4-5 with acid, adding saccharifying enzyme, and performing enzymolysis in water bath at 50-70 deg.C for 1-2 days. Because of the specificity of enzyme, the cellulase only decomposes beta-1, 4 glycosidic bonds, and more alpha-1, 4-and alpha-1, 6-glycosidic bonds in the algal polysaccharides are not enzymolyzed, and the polysaccharides containing alpha-1, 4-and alpha-1, 6-glycosidic bonds in the enzymolysis process of the cellulase still exist in the algal residues and do not enter the supernatant subjected to the enzymolysis of the cellulase in CN 106834358A, therefore, although CN 106834358A carries out the enzymolysis of the cellulase and the enzymolysis of the glucoamylase, the glucoamylase is added into the supernatant after the enzymolysis of the cellulase, so that a large amount of polysaccharides containing alpha-1, 4-and alpha-1, 6-glycosidic bonds are not enzymolyzed, and the problem of incomplete extraction exists.
This application has to draw incompletely to patent number CN 106834358A, causes a large amount of nutrients in the marine alga extravagant, improves, makes and draws more completely, simultaneously, also makes the marine alga essence quality after drawing more stable.
At present, a method for improving the solid content of an extracting solution, retaining more seaweed essence and ensuring the stable quality of a finally prepared product does not exist, and the extracting solution has a good effect of delaying senescence when being applied to cosmetics.
Disclosure of Invention
The invention provides a marine biological activity cosmetic, which solves the technical problems that 1) the cosmetic has the effect of delaying senility; 2) the damage to the effective components of the seaweed is reduced, and more seaweed active substances are reserved; 3) further improving the water-soluble solid content in the seaweed extract and stabilizing the quality of the extracted seaweed essence.
In order to solve the technical problems, the invention adopts the following technical scheme:
a marine bioactive cosmetic comprises seaweed essence, wherein the seaweed essence is prepared by the following steps:
1) enzymolysis: adding seaweed, water and enzyme into a container, and carrying out enzymolysis under the condition that the pH value is 4.2-6.5 to obtain an enzymolysis liquid;
2) seaweed essence: the enzymolysis liquid obtained in the step 1) is seaweed essence, or
Filtering the enzymolysis liquid to obtain filtrate, namely the seaweed essence;
the enzymolysis sequentially comprises cellulase enzymolysis, saccharification enzymolysis and protease enzymolysis;
the enzyme is saccharifying enzyme, cellulase and protease;
the mass ratio of the seaweed to the water to the enzyme in the step 1) is 19-39: 60-80: 0.001-0.5;
the enzymolysis temperature in the step 1) is 26-62 ℃, and the enzymolysis time is 12-96 h.
The cellulose enzymolysis is to add seaweed, water and cellulose into a container, and carry out enzymolysis under the condition that the pH value is 5.5-6.5 to obtain cellulose enzymolysis liquid;
the saccharification enzymatic hydrolysis is to add a saccharifying enzyme into a cellulase enzymolysis liquid, and carry out enzymatic hydrolysis under the condition that the pH is 4.0-4.5 to obtain a saccharification enzymatic hydrolysis liquid;
and in the step of protease enzymolysis, protease is added into the saccharification enzymolysis liquid, and enzymolysis is carried out under the condition that the pH value is 6.0-7.0, so as to obtain the saccharification enzymolysis liquid.
The enzymolysis temperature of the cellulase enzymolysis is 26-35 ℃, and the enzymolysis time is 6-25 h; enzyme deactivation is carried out after enzymolysis, wherein the enzyme deactivation temperature is 90-120 ℃, and the enzyme deactivation time is 2-5 min;
the enzymolysis temperature of the saccharification enzymatic hydrolysis is 55-62 ℃, and the enzymolysis time is 6-48 h; enzyme deactivation is carried out after enzymolysis, wherein the enzyme deactivation temperature is 90-120 ℃, and the enzyme deactivation time is 2-5 min;
the enzymolysis temperature of the protease enzymolysis is 26-35 ℃, and the enzymolysis time is 6-48 h; and enzyme deactivation is carried out after enzymolysis, wherein the enzyme deactivation temperature is 90-120 ℃, and the enzyme deactivation time is 2-5 min.
The protease enzymolysis also comprises the step of introducing nitrogen into the saccharified enzymolysis liquid before enzymolysis until the oxygen content in the saccharified enzymolysis liquid is lower than 0.2 mg/L.
The invention has the following beneficial technical effects:
1. the application can obviously slow down aging.
2. The method avoids the use of a chemical extraction method, can reduce the damage of the chemical extraction on the effective components of the seaweed, and can improve the content of water-soluble solid content in the extract.
3. The cell walls of seaweed are dissolved by cellulase, so that intracellular substances are dissolved out, and alpha-1, 4-and alpha-1, 6-glycosidic bonds are hydrolyzed by further adopting saccharifying enzyme, so that the content of seaweed polysaccharide in an extracting solution is improved; in order to further extract the seaweed, the protease is used for extraction, however, the applicant finds that the color of the extracting solution is gradually deepened and the water-soluble solid content is reduced along with the increase of the temperature and the extension of the extraction time in the extraction process by using the protease; however, protease enzymolysis is carried out at a lower temperature, the color of the extracting solution is not obviously changed, the water-soluble solid content is not obviously reduced, but the color of the extracting solution is changed and gradually deepened along with the prolonging of the storage time, and the water-soluble solid content is reduced; when the enzymolysis of the protease is carried out firstly, the color of the enzymolysis liquid is gradually deepened along with the rise of the temperature and the extension of the extraction time in the process of the saccharification enzymolysis, and the solid content of the water-soluble liquid is reduced. This has the problem that the quality of the product is not stable due to the combined extraction of cellulase, glucoamylase and protease. According to the method, the problem of unstable quality of the extracting solution prepared by jointly extracting cellulase, saccharifying enzyme and protease can be effectively prevented by limiting the enzymolysis temperature of the protease and introducing nitrogen before enzymolysis.
Detailed Description
The present invention is further illustrated by the following specific examples.
Example 1
The marine biological activity skin cream comprises 7% of isopropyl palmitate, 4% of cetyl alcohol, 25% of seaweed essence, 3% of propylene glycol, 0.2% of imidazolidinyl urea, 1.2% of triethanolamine and the balance of water, wherein the seaweed essence is prepared by the following steps:
1) enzymolysis: adding seaweed, water and enzyme into a container, and carrying out enzymolysis to obtain an enzymolysis liquid;
2) seaweed essence: filtering the enzymolysis liquid obtained in the step 1) to obtain filtrate, namely the seaweed essence;
the enzymolysis sequentially comprises cellulase enzymolysis, saccharification enzymolysis and protease enzymolysis;
the enzyme is glucoamylase, cellulase and papain, and the mass ratio of the glucoamylase to the papain is 1:1: 1;
the mass ratio of the seaweed, the water and the enzyme in the step 1) is 30:69.7: 0.3;
step 2), filtering, wherein the mesh diameter of a filter screen is 400 meshes;
the seaweed is dried Porphyra haitanensis.
The cellulase enzymolysis is to add seaweed, water and cellulase into a container, carry out enzymolysis for 12 hours under the conditions that the pH is 6.0 and the enzymolysis temperature is 30 ℃, and inactivate the enzyme for 3min under the condition that the temperature is 100 ℃ to obtain cellulase hydrolysate;
the saccharification enzymatic hydrolysis is to add saccharifying enzyme into a cellulase hydrolysate, carry out enzymatic hydrolysis for 24 hours under the conditions that the pH is 4.2 and the enzymatic hydrolysis temperature is 60 ℃, and inactivate the enzyme for 3min under the condition that the temperature is 100 ℃ to obtain a saccharification enzymatic hydrolysate;
adding papain into the saccharified enzymolysis solution, performing enzymolysis for 24h at pH of 6.5 and enzymolysis temperature of 32 deg.C, and inactivating enzyme at 100 deg.C for 3min to obtain protein enzymolysis solution.
Wherein the activity of the saccharifying enzyme is 50000U/g, the activity of the cellulase is 100000U/g, and the activity of the papain is 50000U/g.
Example 2
The marine biological activity shampoo is composed of 15% of lauryl alcohol ether sulfate sodium salt, 4% of lauryl alcohol sulfate sodium salt, 6% of cocamidopropyl betaine, 10% of seaweed essence, 3% of ethylene glycol stearate, 0.2% of methylisothiazolinone, 100.3% of polyquaternary ammonium salt, 1.2% of triethanolamine and the balance of water, wherein the seaweed essence is prepared by the following steps:
1) enzymolysis: adding seaweed, water and enzyme into a container, and carrying out enzymolysis to obtain an enzymolysis liquid;
2) seaweed essence: filtering the enzymolysis liquid obtained in the step 1) to obtain filtrate, namely the seaweed essence;
the enzyme is glucoamylase, cellulase and papain, and the mass ratio of the glucoamylase to the papain is 1:1: 1;
the mass ratio of the seaweed, the water and the enzyme in the step 1) is 30:69.7: 0.3;
step 2), filtering, wherein the mesh diameter of a filter screen is 400 meshes;
the seaweed is dried Porphyra haitanensis.
The enzymolysis sequentially comprises cellulase enzymolysis, saccharification enzymolysis and protease enzymolysis;
the cellulase enzymolysis is to add seaweed, water and cellulase into a container, carry out enzymolysis for 12 hours under the conditions that the pH is 6.0 and the enzymolysis temperature is 30 ℃, and inactivate the enzyme for 3min under the condition that the temperature is 100 ℃ to obtain cellulase hydrolysate;
the saccharification enzymatic hydrolysis is to add saccharifying enzyme into a cellulase hydrolysate, carry out enzymatic hydrolysis for 24 hours under the conditions that the pH is 4.2 and the enzymatic hydrolysis temperature is 60 ℃, and inactivate the enzyme for 3min under the condition that the temperature is 100 ℃ to obtain a saccharification enzymatic hydrolysate;
and in the step of protease enzymolysis, nitrogen is introduced into the saccharified enzymolysis liquid until the oxygen content in the saccharified enzymolysis liquid is lower than 0.2mg/L, papain is added into the deoxidized saccharified enzymolysis liquid, enzymolysis is carried out for 24 hours under the conditions that the pH is 6.5 and the enzymolysis temperature is 32 ℃, and enzyme is inactivated for 3 minutes under the condition that the temperature is 100 ℃, so that the protein enzymolysis liquid is obtained.
Wherein the activity of the saccharifying enzyme is 50000U/g, the activity of the cellulase is 100000U/g, and the activity of the papain is 50000U/g.
Example 3
The marine biological activity skin cream comprises 8% of isopropyl palmitate, 5% of cetyl alcohol, 3% of seaweed essence, 4% of propylene glycol, 0.2% of imidazolidinyl urea, 1.1% of triethanolamine and the balance of water, wherein the seaweed essence is prepared by the following steps:
1) enzymolysis: adding seaweed, water and enzyme into a container, and carrying out enzymolysis to obtain an enzymolysis liquid;
2) seaweed essence: filtering the enzymolysis liquid obtained in the step 1) to obtain filtrate, namely the seaweed essence;
the enzyme is glucoamylase, cellulase and papain, and the mass ratio of the glucoamylase to the papain is 1:3: 1;
the mass ratio of the seaweed, the water and the enzyme in the step 1) is 34:65.993: 0.007;
step 2), filtering, wherein the mesh diameter of a filter screen is 300 meshes;
the seaweed is kelp.
The enzymolysis sequentially comprises cellulase enzymolysis, saccharification enzymolysis and protease enzymolysis;
the cellulase enzymolysis is to add seaweed, water and cellulase into a container, carry out enzymolysis for 15h under the conditions that the pH is 5.5 and the enzymolysis temperature is 32 ℃, and inactivate the enzyme for 3min under the condition that the temperature is 90 ℃ to obtain cellulase hydrolysate;
the saccharification enzymatic hydrolysis is to add saccharifying enzyme into a cellulase hydrolysate, carry out enzymatic hydrolysis for 36 hours under the conditions that the pH is 4.4 and the enzymatic hydrolysis temperature is 57 ℃, and inactivate the enzyme for 3min under the condition that the temperature is 100 ℃ to obtain a saccharification enzymatic hydrolysate;
and in the step of protease enzymolysis, nitrogen is introduced into the saccharified enzymolysis liquid until the oxygen content in the saccharified enzymolysis liquid is 0.1mg/L, papain is added into the deoxidized saccharified enzymolysis liquid, enzymolysis is carried out for 12 hours under the conditions that the pH is 7.0 and the enzymolysis temperature is 28 ℃, and enzyme is inactivated for 1min under the condition that the temperature is 120 ℃, so that the protein enzymolysis liquid is obtained.
Wherein the activity of the saccharifying enzyme is 50000U/g, the activity of the cellulase is 100000U/g, and the activity of the papain is 50000U/g.
Example 4
The marine biological activity eye cream comprises 5% of cetyl alcohol, 6% of isopropyl palmitate, 5% of octadecanol, 0.4% of carbomer, 20% of seaweed essence, 10% of butanediol, 0.2% of imidazolidinyl urea and the balance of water, wherein the seaweed essence is prepared by the following steps:
1) enzymolysis: adding seaweed, water and enzyme into a container, and carrying out enzymolysis to obtain an enzymolysis liquid;
2) seaweed essence: obtaining the enzymatic hydrolysate obtained in the step 1) as seaweed essence;
the enzyme is glucoamylase, cellulase and papain, and the mass ratio of the glucoamylase to the papain is 2:1: 2;
the mass ratio of the seaweed, the water and the enzyme in the step 1) is 27:72.97: 0.03;
the seaweed is Eucheuma Gelatinosum.
The enzymolysis sequentially comprises cellulase enzymolysis, saccharification enzymolysis and protease enzymolysis;
the cellulase enzymolysis is to add seaweed, water and cellulase into a container, carry out enzymolysis for 8 hours under the conditions that the pH is 6.5 and the enzymolysis temperature is 28 ℃, and inactivate the enzyme for 2min under the condition that the temperature is 100 ℃ to obtain cellulase hydrolysate;
the saccharification enzymatic hydrolysis is to add saccharifying enzyme into a cellulase hydrolysate, carry out enzymatic hydrolysis for 34h under the conditions that the pH is 4.4 and the enzymatic hydrolysis temperature is 62 ℃, and inactivate the enzyme for 2min under the condition that the temperature is 100 ℃ to obtain a saccharification enzymatic hydrolysate;
and in the step of protease enzymolysis, nitrogen is introduced into the saccharified enzymolysis liquid until the oxygen content in the saccharified enzymolysis liquid is lower than 0.2mg/L, thiol protease is added into the deoxidized saccharified enzymolysis liquid, enzymolysis is carried out for 36 hours under the conditions that the pH is 6.8 and the enzymolysis temperature is 32 ℃, and enzyme is inactivated for 3 minutes under the condition that the temperature is 100 ℃, so that the protein enzymolysis liquid is obtained.
Wherein the activity of the saccharifying enzyme is 40000U/g, the activity of the cellulase is 150000U/g, and the activity of the thiol protease is 40000U/g.
Example 5
The marine biological activity skin moisturizer and the marine biological activity skin moisturizer are composed of 9% of isopropyl palmitate, 3% of cetyl alcohol, 30% of seaweed essence, 4% of propylene glycol, 0.2% of imidazolidinyl urea, 1.1% of triethanolamine and the balance of water, wherein the seaweed essence is prepared according to the following steps:
the seaweed essence is prepared according to the following steps:
1) enzymolysis: adding seaweed, water and enzyme into a container, and carrying out enzymolysis to obtain an enzymolysis liquid;
2) seaweed essence: filtering the enzymolysis liquid obtained in the step 1) to obtain filtrate, namely the seaweed essence;
the enzyme is glucoamylase, cellulase and papain, and the mass ratio of the glucoamylase to the papain is 2:2: 1;
the mass ratio of the seaweed, the water and the enzyme in the step 1) is 28:71.95: 0.05;
the seaweed is Sargassum.
The enzymolysis comprises cellulase enzymolysis, saccharification enzymolysis and subtilisin in sequence;
the cellulase enzymolysis is to add seaweed, water and cellulase into a container, carry out enzymolysis for 10 hours under the conditions that the pH is 5.7 and the enzymolysis temperature is 27 ℃, and inactivate the enzyme for 1min under the condition that the temperature is 110 ℃ to obtain cellulase hydrolysate;
the saccharification enzymatic hydrolysis is to add saccharifying enzyme into a cellulase hydrolysate, carry out enzymatic hydrolysis for 32 hours under the conditions that the pH is 4.1 and the enzymatic hydrolysis temperature is 58 ℃, and inactivate the enzyme for 2min under the condition that the temperature is 100 ℃ to obtain a saccharification enzymatic hydrolysate;
and in the step of protease enzymolysis, nitrogen is introduced into the saccharified enzymolysis liquid until the oxygen content in the saccharified enzymolysis liquid is lower than 0.2mg/L, subtilisin is added into the deoxidized saccharified enzymolysis liquid, enzymolysis is carried out for 28 hours under the conditions that the pH is 6.6 and the enzymolysis temperature is 30 ℃, and the protease is inactivated for 3 minutes under the condition that the temperature is 100 ℃ to obtain the proteolysis liquid.
Wherein the activity of saccharifying enzyme is 60000U/g, the activity of cellulase is 150000U/g, and the activity of subtilisin is 40000U/g.
Example 6
The marine biological activity facial cleanser consists of 12% of lauryl alcohol ether sulfate sodium salt, 2% of bamboo salt, 3% of lauryl alcohol sulfate sodium salt, 5% of cocamidopropyl betaine, 15% of seaweed essence, 3% of ethylene glycol stearate, 0.2% of methylisothiazolinone, 100.3% of polyquaternary ammonium salt, 1.2% of triethanolamine and the balance of water, wherein the seaweed essence is prepared by the following steps:
1) enzymolysis: adding seaweed, water and enzyme into a container, and carrying out enzymolysis to obtain an enzymolysis liquid;
2) seaweed essence: filtering the enzymolysis liquid obtained in the step 1) to obtain filtrate, namely the seaweed essence;
the enzyme is glucoamylase, cellulase and papain, and the mass ratio of the glucoamylase to the papain is 2:2: 1;
the mass ratio of the seaweed, the water and the enzyme in the step 1) is 28:71.95: 0.05;
the seaweed is Sargassum.
The enzymolysis comprises cellulase enzymolysis, saccharification enzymolysis and subtilisin in sequence;
the cellulase enzymolysis is to add seaweed, water and cellulase into a container, carry out enzymolysis for 10 hours under the conditions that the pH is 5.7 and the enzymolysis temperature is 27 ℃, and inactivate the enzyme for 1min under the condition that the temperature is 110 ℃ to obtain cellulase hydrolysate;
the saccharification enzymatic hydrolysis is to add saccharifying enzyme into a cellulase hydrolysate, carry out enzymatic hydrolysis for 32 hours under the conditions that the pH is 4.1 and the enzymatic hydrolysis temperature is 58 ℃, and inactivate the enzyme for 2min under the condition that the temperature is 100 ℃ to obtain a saccharification enzymatic hydrolysate;
and in the step of protease enzymolysis, nitrogen is introduced into the saccharified enzymolysis liquid until the oxygen content in the saccharified enzymolysis liquid is lower than 0.2mg/L, subtilisin is added into the deoxidized saccharified enzymolysis liquid, enzymolysis is carried out for 28 hours under the conditions that the pH is 6.6 and the enzymolysis temperature is 30 ℃, and the protease is inactivated for 3 minutes under the condition that the temperature is 100 ℃ to obtain the proteolysis liquid.
Wherein the activity of saccharifying enzyme is 60000U/g, the activity of cellulase is 150000U/g, and the activity of subtilisin is 40000U/g.
Example 8
The marine bioactive cosmetic is composed of 16% of lauryl alcohol ether sulfate sodium salt, 4% of lauryl alcohol sulfate sodium salt, 6% of cocamidopropyl betaine, 35% of seaweed essence, 3% of ethylene glycol stearate, 0.2% of methylisothiazolinone, 100.3% of polyquaternary ammonium salt, 1.2% of triethanolamine and the balance of water, wherein the seaweed essence is prepared by the following steps:
1) enzymolysis: adding seaweed, water and enzyme into a container, and carrying out enzymolysis to obtain an enzymolysis liquid;
2) seaweed essence: filtering the enzymolysis liquid obtained in the step 1) to obtain filtrate, namely the seaweed essence;
the enzyme is glucoamylase, cellulase and papain, and the mass ratio of the glucoamylase to the papain is 2:2: 1;
the mass ratio of the seaweed, the water and the enzyme in the step 1) is 28:71.95: 0.05;
the seaweed is Sargassum.
The enzymolysis comprises cellulase enzymolysis, saccharification enzymolysis and subtilisin in sequence;
the cellulase enzymolysis is to add seaweed, water and cellulase into a container, carry out enzymolysis for 10 hours under the conditions that the pH is 5.7 and the enzymolysis temperature is 27 ℃, and inactivate the enzyme for 1min under the condition that the temperature is 110 ℃ to obtain cellulase hydrolysate;
the saccharification enzymatic hydrolysis is to add saccharifying enzyme into a cellulase hydrolysate, carry out enzymatic hydrolysis for 32 hours under the conditions that the pH is 4.1 and the enzymatic hydrolysis temperature is 58 ℃, and inactivate the enzyme for 2min under the condition that the temperature is 100 ℃ to obtain a saccharification enzymatic hydrolysate;
and in the step of protease enzymolysis, nitrogen is introduced into the saccharified enzymolysis liquid until the oxygen content in the saccharified enzymolysis liquid is lower than 0.2mg/L, subtilisin is added into the deoxidized saccharified enzymolysis liquid, enzymolysis is carried out for 28 hours under the conditions that the pH is 6.6 and the enzymolysis temperature is 30 ℃, and the protease is inactivated for 3 minutes under the condition that the temperature is 100 ℃ to obtain the proteolysis liquid.
Wherein the activity of saccharifying enzyme is 60000U/g, the activity of cellulase is 150000U/g, and the activity of subtilisin is 40000U/g.
The beneficial effects of the present invention are further illustrated below in conjunction with experimental data:
test material
1.1 test site: the detection was made by Sanfeng (tobacco pipe) agriculture science and technology Co.
1.2 test detection: water soluble solids content, algal polysaccharide content, free amino acid content and auxin (IAA) content.
1.3 test materials: the seaweed essence prepared in the comparative example 1 (except that the extraction method of the seaweed essence uses 2% sulfuric acid instead of protease for treatment at 100 ℃ for 1 hour, other preparation methods are all consistent with those in the example 1), the seaweed essence prepared in the comparative example 2 (except that the extraction method of the seaweed essence uses 60 ℃ protease enzymolysis temperature, other preparation methods are all consistent with those in the example 1), the seaweed essence prepared in the comparative example 3 (except that the extraction method of the seaweed essence sequentially uses protease enzymolysis, cellulase enzymolysis and carbohydrase enzymolysis, other preparation methods are all consistent with those in the example 1), and the seaweed essence prepared in the example 1.
1.4 Experimental implementation: solid content is measured according to the solid content of the coating GB 1725-79; the seaweed polysaccharide is detected by a sulfuric acid-phenol method; detecting the content of free amino acid according to the determination of the free amino acid in the GB/T30987-2014 plant; the content of auxin (IAA) is measured according to a wheat coleoptile cutting biological test method.
The present application is consistent in other implementations except for differences in the respective processes.
2 results and analysis
The water-soluble solids content, algal polysaccharide content, free amino acid content and auxin (IAA) content are shown in Table 1
TABLE 1
| Solid content (%) | Algal polysaccharide (%) | Auxin (mg/L) | Free amino acid (%) | |
| Comparative example 1 | 20.9 | 13.8 | 0 | 6.5 |
| Comparative example 2 | 19.1 | 12.6 | 9.8 | 5.6 |
| Comparison 3 | 18.5 | 12.3 | 9.6 | 5.4 |
| Example 1 | 21.4 | 14.1 | 10.7 | 6.7 |
Note: according to the application, the content of soluble sugar, cellulose and crude protein in porphyra haitanensis is 47.6%, 1.6% and 28.6% respectively by dry basis detection.
As can be seen from comparison of the data of comparative example 1 (except that protease hydrolysis was replaced by treatment with 2% sulfuric acid at 100 ℃ C. for 1 hour, the preparation method was identical to that of example 1) and the seaweed essence prepared in example 2 in Table 1, the treatment in a hot environment with the addition of sulfuric acid destroyed a part of the effective components of the seaweed, i.e., the content of auxin, resulting in incomplete extraction. As can be seen from comparison of the data of the seaweed essence prepared in comparison 2 (except that the enzymolysis temperature of the protease is 60 ℃, the other preparation methods are the same as those in example 1) and the seaweed essence prepared in example 1, although the optimal enzymolysis temperature of the papain is 55-65 ℃, the temperature can reduce the contents of seaweed polysaccharide and free amino acid at the same time, that is, the seaweed polysaccharide and the free amino acid are generated by chemical reaction at the temperature, and incomplete extraction is also caused; the comparison of the data of the seaweed essence prepared by the comparison 3 (except that the enzymolysis sequence is protease enzymolysis, cellulase enzymolysis and saccharification enzymolysis in turn, and other preparation methods are all consistent with those of the example 1) and the data of the seaweed essence prepared by the example 1 shows that the change of the enzymolysis sequence can also influence the change of the finally extracted components, so that the seaweed essence prepared by the example 1 has a better extraction effect.
Experiment two
1.1 test site: the detection was made by Sanfeng (tobacco pipe) agriculture science and technology Co.
1.2 test detection: initial water-soluble solid content, final water-soluble solid content, initial algal polysaccharide content, final algal polysaccharide content, initial free amino acid content, final free amino acid content, initial auxin content, and final auxin content.
1.3 test materials: comparative example 4 (except that the preparation process of the seaweed essence is not hydrolyzed by protease, the preparation method is the same as that of example 1) and the seaweed essences prepared in examples 1 and 2.
1.4 Experimental implementation: the initial content corresponds to the content of the seaweed essence produced immediately after production; the final content is the algal polysaccharide content after being placed indoors for 6 months in a 1L polyethylene bottle; the laboratory temperature was 25 ℃.
1.5 detection method: solid content is measured according to the solid content of the coating GB 1725-79; the seaweed polysaccharide is detected by a sulfuric acid-phenol method; detecting the content of free amino acid according to the determination of the free amino acid in the GB/T30987-2014 plant; the content of auxin (IAA) is measured according to a wheat coleoptile cutting biological test method.
The present application is consistent in other implementations except for differences in the respective processes.
2 results and analysis
The initial water-soluble solid content, the final water-soluble solid content, the initial algal polysaccharide content, the final algal polysaccharide content, the initial free amino acid content, the final free amino acid content, the initial auxin content and the final auxin content are shown in Table 2
TABLE 2
| Example 1 | Example 2 | Comparative example 4 | |
| Initial Water-soluble solids (%) | 21.4 | 21.4 | 14.5 |
| Final Water-soluble solids (%) | 20.1 | 21.3 | 14.5 |
| Initial algal polysaccharide content (%) | 14.1 | 14.1 | 14.1 |
| Final algal polysaccharide content (%) | 13.2 | 14.0 | 14.1 |
| Initial free amino acid content (%) | 6.7 | 6.7 | 0.8 |
| Final free amino acid content (%) | 6.1 | 6.7 | 0.8 |
| Content of auxin (mg/L) | 10.7 | 10.7 | 3.1 |
| Final auxin content (mg/L) | 8.6 | 10.6 | 3.1 |
As can be seen from the comparison of the data of the seaweed essence prepared in comparison 4 (except that the seaweed essence is not hydrolyzed by protease in the preparation process, the preparation method is the same as that of the seaweed essence prepared in example 1) with the data of the seaweed essence prepared in example 1, the quality of the seaweed essence prepared in comparison 4 is stable through enzymolysis by cellulase and carbohydrase, while the quality of the seaweed essence prepared in example 1 is unstable after the enzymolysis by protease.
The data comparison of the seaweed essence prepared in the embodiment 1 and the seaweed essence prepared in the embodiment 2 in the table 2 shows that the seaweed essence prepared in the embodiment 1 has the problem of unstable quality after being placed for a long time, and the stability of the product quality can be effectively improved by introducing nitrogen.
Experiment three
1.1 test site: the detection was made by Sanfeng (tobacco pipe) agriculture science and technology Co.
1.2 test detection: DPPH clearance and water loss.
1.3 test materials: comparative example 1 (except that the extraction method of the marine algae essence is changed to the method of treating the marine algae essence with 2% sulfuric acid at 100 ℃ for 1 hour by performing enzymolysis on protease, the preparation method is the same as that of example 1), and the marine algae essences prepared in the first example 1 and the first example 2; the essence of seaweed prepared in final example 1 and final example 2.
1.4 Experimental implementation: the DPPH clearance rate adopts a diphenylbitter acyl radical analysis method, and the mass ratio of a sample to be detected to ethanol is 1: 100; skin Water loss tester Tewameter TM 300.
The experimental temperature is 25 ℃; humidity is 40% -60%, and real-time dynamic monitoring is carried out.
The tested part can not be used for any product (cosmetics or external medicines) 2 days before testing, and can not contact water within 2 h. Before testing, the subjects needed to clean the inner sides of the forearms of both hands uniformly by wiping them clean with a clean facial tissue.
The part of the inner side of the forearm of the subject 5cm away from the base of the palm is an experimental part, and each experimental part is 9cm2The same arm marks 6 test areas, each 1.5cm apart.
The test should be rested for 30min in a standard room before official testing. The forearm is exposed and placed in a test state to keep relaxed.
The product application area and the blank control area should be randomly distributed in the left and right arm calibration areas to ensure that all product and blank area positions are statistically balanced.
The TM300 test water loss was measured in duplicate 6 times per area, averaged and recorded as a blank value.
The test sample is 2.0 +/-0.1 mg/cm2The amount of (c) was applied in a single pass, and the sample was evenly spread over the test area using a latex finger cuff.
The water loss was measured using TM300 for 1h, 2h, 3h and 4h of the applied samples, 6 times each, and the average was taken.
The number of test subjects per experimental treatment was 10, 40 total, between 20 and 30 years of age, combined randomly.
The present application is consistent in other implementations except for differences in the respective processes.
2 results and analysis
The results of the radical (DPPH) clearance (%) and water loss (%) measurements are shown in Table 3
TABLE 3
As can be seen from the comparison of the data in the first example 1 and the data in the last example 1 in table 3, the seaweed essence in the last example 1 is significantly reduced in effect after being placed for 6 months, while the seaweed essences prepared in the first example 1, the first example 2 and the last example 2 are substantially consistent in effect, and it can be seen that the enzymolysis temperature of the protease and the introduction of nitrogen before the enzymolysis can stabilize the quality of the seaweed essence. As can be seen from comparison of the data in comparative example 1 and example 1, the present application has the effect of better DPPH scavenging and water loss reduction, while retaining more seaweed actives.
Claims (4)
1. The marine bioactive cosmetic is characterized by comprising seaweed essence, wherein the seaweed essence is prepared according to the following steps:
1) enzymolysis: adding seaweed, water and enzyme into a container, and carrying out enzymolysis under the condition that the pH value is 4.2-6.5 to obtain an enzymolysis liquid;
2) seaweed essence: the enzymolysis liquid obtained in the step 1) is seaweed essence, or
Filtering the enzymolysis liquid to obtain filtrate, namely the seaweed essence;
the enzymolysis sequentially comprises cellulase enzymolysis, saccharification enzymolysis and protease enzymolysis;
the enzyme is saccharifying enzyme, cellulase and protease;
the mass ratio of the seaweed to the water to the enzyme in the step 1) is 19-39: 60-80: 0.001-0.5;
the enzymolysis temperature in the step 1) is 26-62 ℃, and the enzymolysis time is 12-96 h.
2. The marine bioactive cosmetic as claimed in claim 2, wherein the cellulase enzymolysis is to add seaweed, water and cellulase into a container, and carry out enzymolysis under the condition that the pH is 5.5-6.5 to obtain cellulase hydrolysate;
the saccharification enzymatic hydrolysis is to add a saccharifying enzyme into a cellulase enzymolysis liquid, and carry out enzymatic hydrolysis under the condition that the pH is 4.0-4.5 to obtain a saccharification enzymatic hydrolysis liquid;
and in the step of protease enzymolysis, protease is added into the saccharification enzymolysis liquid, and enzymolysis is carried out under the condition that the pH value is 6.0-7.0, so as to obtain the saccharification enzymolysis liquid.
3. The marine bioactive cosmetic as claimed in claim 2, wherein the enzymolysis temperature of the cellulase is 26-35 ℃, and the enzymolysis time is 6-25 h; enzyme deactivation is carried out after enzymolysis, wherein the enzyme deactivation temperature is 90-120 ℃, and the enzyme deactivation time is 2-5 min;
the enzymolysis temperature of the saccharification enzymatic hydrolysis is 55-62 ℃, and the enzymolysis time is 6-48 h; enzyme deactivation is carried out after enzymolysis, wherein the enzyme deactivation temperature is 90-120 ℃, and the enzyme deactivation time is 2-5 min;
the enzymolysis temperature of the protease enzymolysis is 26-35 ℃, and the enzymolysis time is 6-48 h; and enzyme deactivation is carried out after enzymolysis, wherein the enzyme deactivation temperature is 90-120 ℃, and the enzyme deactivation time is 2-5 min.
4. The marine bioactive cosmetic of any of claims 1 to 3, wherein the enzymatic hydrolysis by the protease further comprises introducing nitrogen gas into the saccharified enzymatic hydrolysate before the enzymatic hydrolysis until the oxygen content in the saccharified enzymatic hydrolysate is less than 0.2 mg/L.
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| CN202110106135.6A CN112451429A (en) | 2021-01-26 | 2021-01-26 | Marine biological active cosmetic |
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| CN202110106135.6A CN112451429A (en) | 2021-01-26 | 2021-01-26 | Marine biological active cosmetic |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104177136A (en) * | 2013-05-21 | 2014-12-03 | 徐波勇 | Method for extracting active substances for fertilizers from algae |
| CN105218248A (en) * | 2015-10-23 | 2016-01-06 | 青岛聚大洋藻业集团有限公司 | A kind of preparation method of biological and ecological methods to prevent plant disease, pests, and erosion active liquid seaweed fertilizer |
| CN106834358A (en) * | 2017-03-21 | 2017-06-13 | 青岛大学 | A kind of method that Efficient Conversion algal polysaccharides prepare bio-ethanol |
| CN110615856A (en) * | 2019-08-14 | 2019-12-27 | 山东劲脉植物细胞信息技术有限公司 | Extraction method of seaweed active ingredients |
-
2021
- 2021-01-26 CN CN202110106135.6A patent/CN112451429A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104177136A (en) * | 2013-05-21 | 2014-12-03 | 徐波勇 | Method for extracting active substances for fertilizers from algae |
| CN105218248A (en) * | 2015-10-23 | 2016-01-06 | 青岛聚大洋藻业集团有限公司 | A kind of preparation method of biological and ecological methods to prevent plant disease, pests, and erosion active liquid seaweed fertilizer |
| CN106834358A (en) * | 2017-03-21 | 2017-06-13 | 青岛大学 | A kind of method that Efficient Conversion algal polysaccharides prepare bio-ethanol |
| CN110615856A (en) * | 2019-08-14 | 2019-12-27 | 山东劲脉植物细胞信息技术有限公司 | Extraction method of seaweed active ingredients |
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