CN106923337A - A kind of preparation method and applications of spirulina full agonist - Google Patents
A kind of preparation method and applications of spirulina full agonist Download PDFInfo
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The present invention discloses a kind of preparation method and applications of spirulina full agonist.With spirulina as raw material, using opposed polarity（From small to large）The method extraction such as solvent extraction and compound protease enzymolysis prepares spirulina full agonist：First extracted with 85wt% 95wt% ethanol, extracted with 55wt% 65wt% ethanol again, then water extraction, finally water extraction residue is digested, again respectively through centrifugation, filtering, decompression, concentration, dry prepared spirulina 85wt% 95wt% ethanol extracts, spirulina 55wt% 65wt% ethanol extracts, spirulina water extract and spirulina enzymolysis thing, i.e.,：Spirulina full agonist.Verified through pharmacodynamic experiment, full agonist can significantly improve the disorders of lipid metabolism of high fat diet induction, especially, can significantly reduce T-CHOL in serum（TC）, triglyceride（TG）And LDL-C（LDL‑C）Level.
Description
Technical field
Field is extracted the invention belongs to spirulina-active material, and in particular to a kind of preparation side of spirulina full agonist
Method and its application.
Background technology
Spirulina is the classic pure natural protein food source that the mankind are had found, its protein content is up to 60%-
70%, it is the high-quality algae of a kind of " food and medicine consangunity ", contains abundant nutritional ingredient, such as protein, fat, vitamin, mineral
Matter, chloroplaset, carrotene and polysaccharose substance, thus it is referred to as " 21 century food ".Research finds that spirulina has notable
Ground immunological regulation, antifatigue, passage, nourishing blood, resist oxygen lack, hypotensive, reducing blood sugar and blood lipid, the tumour that suppresses, liver protecting, promotion are micro-
The absorption of mineral matter is measured, is lost weight and is overcome the plurality of health care functions such as malnutrition.
Diabetes and hypertension are a kind of common disease and frequently-occurring disease in China, are at present in the world after tumour and cardiovascular disease
The disease of the harm human health being number three after after being ill.Wherein diabetes are one group due to defect of insulin secretion or pancreas islet
The metabolic disease with the characteristics of hyperglycaemia caused by element effect obstacle, persistent high blood sugar and long-term metabolic disorder etc. can cause complete
Body histoorgan, particularly eye, kidney, the infringement and its dysfunction and exhaustion of angiocarpy and nervous system.Severe patient can cause
Lose acute complicationses DKA and the Hyperosmotic comas such as water and rock-soil coupling and acid-base imbalance.With rhythm of life plus
Hurry up, dietary structure is unreasonable, onset diabetes rate is in rising trend, and age of onset also tends to rejuvenation.Hyperglycaemia, high fat of blood with
And the cardiovascular and cerebrovascular disease for being triggered increasingly endangers the health of the mankind, ASSOCIATE STATISTICS, every year people about up to ten thousand die from this
A little diseases, therefore hyperglycaemia high fat of blood, hypertension increasingly become focus of concern, how to prevent diabetes, artery hard
Change and cardiovascular and cerebrovascular disease turns into a problem in the urgent need to address, and as high fat of blood, diabetes and angiocardiopathy are closed
The research of system, it has been recognized that reducing blood lipid, the hypoglycemic risk to reducing angiocardiopathy have great importance.
There is reducing blood lipid to drop blood for the full agonist of the spirulina that the present invention extracts different gradients and chlorella, ginkgo etc.
The extract of the natural materials of sugar mixes the health food for being made and easily taking easy absorption, and cycloheptaamylose is added in addition
To remove the fishy smell of spirulina, improve taste, make people be more easy to receive, this health products has very big treatment to make to " three high " illness
With and for spirulina deep development using there is certain theoretical foundation.
Through retrieval, in " a kind of health biscuits for lowering blood glucose and lipid " at the beginning of spirulina reducing blood sugar and blood lipid field You Lu states
（Number of patent application 201210085881.2）, by spirulina powder and other can hypoglycemic material be made a kind of health-caring biscuit, and
The product that will be made is used in enhancing body immunity, treats stomach disease, dehumidifying aspect；A kind of " hypoglycemic nutrition of spirulina in Sun Man pools
Oil and preparation method thereof "（Number of patent application 201610488537.6）The spirulina polysaccharide of extraction and other material polysaccharide are carried
Take liquid compounding and nutritional oil be obtained, with it is nutritious comprehensive the characteristics of, and with hypoglycemic effect.
The active material that there is spirulina in the technology in the field at present prepares incomplete, the low problem of utilization rate.Mesh
Before, there is not yet spirulina is used into serial solvent（Polarity is from small to large）Extraction prepares the method report of active material, and this side
Production of the method to heavy industrialization has good DEVELOPMENT PROSPECT.
The content of the invention
It is an object of the invention in view of the shortcomings of the prior art, provide a kind of spirulina full agonist preparation method and
Its application.The present invention with spirulina as raw material, opposed polarity（From small to large）Extract and compound protease enzymolysis prepares spirulina
Full agonist, " monarch " theory of traditional Chinese medical science according to the traditional Chinese medical science, obtained spirulina full agonist has phase with other
The health food prepared with the integration of drinking and medicinal herbs active component compounding of effect has following effect：Auxiliary hyperglycemic blood fat and guarantor
Liver, resists cardiovascular and cerebrovascular disease, improves body immunity.
To achieve the above object, the present invention is adopted the following technical scheme that：
A kind of preparation method of spirulina full agonist, with spirulina as raw material, using opposed polarity（From small to large）It is molten
Agent extraction and compound protease enzymolysis prepare spirulina full agonist：
First extracted with 85wt%-95wt% ethanol, 85wt%-95wt% ethanol is then extracted into residue 55wt%-65wt% ethanol
Extract, then by 55wt%-65wt% ethanol extract residue water extraction, finally by water extraction residue digest, by above-mentioned alcohol extracting, water extraction and
The extract solution that enzymolysis is obtained is obtained the extraction of spirulina 85wt%-95wt% ethanol through being centrifuged, filtering, depressurize, concentrate and dry respectively
Thing, spirulina 55wt%-65wt% ethanol extracts, spirulina water extract and spirulina enzymolysis thing, i.e.,：Spirulina is entirely active
Material；Specifically include following steps：
A. spirulina fine powder is first used into 85wt%-95wt% ethanol microwave and ultrasonic wave synergic extraction or ultrasonic wave added in 45-70 DEG C
0.5-3h is extracted, filtering obtains spirulina 85wt%-95wt% alcohol extracts；
B. the spirulina residue utilization 55wt%-65wt% ethanol that the 85wt%-95wt% ethanol that will be obtained is extracted is at 45-70 DEG C
Microwave and ultrasonic wave synergic extraction or ultrasound assisted extraction 0.5-3h are carried out, filtering obtains spirulina 55wt%-65wt% alcohol extracts；
C. the spirulina residue utilization deionized water that the 55wt%-65wt% ethanol that will be obtained again is extracted is carried out at 70-100 DEG C
Microwave and ultrasonic wave synergic extraction or ultrasound assisted extraction 0.5-3h, filtering obtain spirulina Aqueous extracts；
D. the spirulina water extraction residue utilization compound protease of acquisition is carried out into enzymolysis 0.5-3h at 37-65 DEG C again, filtering with
Obtain the enzyme-extracting solution of spirulina；
E. the extract solution for step A-D being prepared is obtained spirulina through centrifugation, filtering, decompression, concentration and after drying respectively
85wt%-95wt% ethanol extracts, spirulina 55wt%-65wt% ethanol extracts, spirulina water extract and spirulina enzyme
Solution thing, i.e.,：Spirulina full agonist.
Extraction solvent and the envelope-bulk to weight ratio for extracting material in step A-C（v:w）It is 10:1-50:1.
Compound protease addition described in step D is the 0.1-2wt% of spirulina water extraction residue.
The present invention also protects the application of obtained spirulina full agonist, takes the obtained full active matter of spirulina
One or more active material compoundings for having identical effect with other in matter are prepared such as oral liquid, capsule, tablet, electuary,
The nutrient functional foods of the different dosage forms of solid beverage.
The beneficial effects of the present invention are：
（1）The present invention with spirulina as raw material, opposed polarity（From small to large）Extract and compound protease enzymolysis prepares spirulina
Full agonist；
（2）" monarch " theory of traditional Chinese medical science according to the traditional Chinese medical science, has identical with obtained spirulina full agonist with other
Health food prepared by the integration of drinking and medicinal herbs active component compounding of effect has following effect：Enrich blood, pre- preventing obesity, auxiliary drop blood
Glucemia fat and liver protection, resist cardiovascular and cerebrovascular disease, improve body immunity.
（3）Present invention process is reliable and stable, and repeatability is high, practical, is suitable for large-scale industrial production.
Brief description of the drawings
Fig. 1 is spirulina opposed polarity extract and compound protease zymolyte（Full agonist）High fat diet is induced
The influence of obese rat blood lipid level.
Specific embodiment
Below in conjunction with specific embodiment, the present invention will be further described, but the present invention is not limited only to these embodiments.
Embodiment 1
A kind of preparation method of spirulina full agonist, with spirulina as raw material, using opposed polarity（From small to large）It is molten
Agent extraction and compound protease enzymolysis prepare spirulina full agonist：
First extracted with 85wt% ethanol, 85wt% ethanol is then extracted into residue 65wt% ethanol and is extracted, then by 65wt% second
Alcohol extracting residue water extraction, finally by water extraction residue digest, the extract solution that above-mentioned alcohol extracting, water extraction and enzymolysis are obtained respectively pass through from
The heart, filtering, decompression, concentration and dry prepared spirulina 85wt% ethanol extracts, spirulina 65wt% ethanol extracts, spirulina
Water extract and spirulina enzymolysis thing, i.e.,：Spirulina full agonist；Specifically include following steps：
A. spirulina fine powder is first used into 85wt% ethanol microwave and ultrasonic wave synergic extraction 3h in 45 DEG C, filtering obtains spirulina
85wt% alcohol extracts；
B. the spirulina residue utilization 65wt% ethanol that the 85wt% ethanol of acquisition is extracted is carried out into ultrasound-microwave association at 45 DEG C
With extraction 3h, filtering obtains spirulina 65wt% alcohol extracts；
C. the spirulina residue utilization deionized water that the 65wt% ethanol that will be obtained again is extracted carries out ultrasound-microwave association at 70 DEG C
With extraction 3h, filtering obtains spirulina Aqueous extracts；
D. the spirulina water extraction residue utilization 0.2wt% compound proteases of acquisition are carried out into enzymolysis 3h at 37 DEG C again, filtering with
Obtain the enzyme-extracting solution of spirulina；
E. the extract solution for step A-D being prepared is obtained spirulina through centrifugation, filtering, decompression, concentration and after drying respectively
85wt% ethanol extracts, spirulina 65wt% ethanol extracts, spirulina water extract and spirulina enzymolysis thing, i.e.,：Spiral
Algae full agonist.
Extraction solvent and the envelope-bulk to weight ratio for extracting material in step A-C（v:w）It is 20:1.
Embodiment 2
A kind of preparation method of spirulina full agonist, with spirulina as raw material, using opposed polarity（From small to large）It is molten
Agent extraction and compound protease enzymolysis prepare spirulina full agonist：
First extracted with 95wt% ethanol, 95wt% ethanol is then extracted into residue 55wt% ethanol and is extracted, then by 55wt% second
Alcohol extracting residue water extraction, finally by water extraction residue digest, the extract solution that above-mentioned alcohol extracting, water extraction and enzymolysis are obtained respectively pass through from
The heart, filtering, decompression, concentration and dry prepared spirulina 95wt% ethanol extracts, spirulina 55wt% ethanol extracts, spirulina
Water extract and spirulina enzymolysis thing, i.e.,：Spirulina full agonist；Specifically include following steps：
A. spirulina fine powder is first used into 95wt% ethanol microwave and ultrasonic wave synergic extraction 0.5h in 70 DEG C, filtering obtains spirulina
95wt% alcohol extracts；
B. the spirulina residue utilization 55wt% ethanol that the 95wt% ethanol of acquisition is extracted is carried out into ultrasound-microwave association at 70 DEG C
With extraction 0.5h, filtering obtains spirulina 55wt% alcohol extracts；
C. the spirulina residue utilization deionized water that the 55wt% ethanol that will be obtained again is extracted carries out ultrasound-microwave at 100 DEG C
Synergic solvent extraction 0.5h, filtering obtains spirulina Aqueous extracts；
D. the spirulina water extraction residue utilization 2wt% compound proteases of acquisition are carried out into enzymolysis 0.5h at 65 DEG C again, filtering with
Obtain the enzyme-extracting solution of spirulina；
E. the extract solution for step A-D being prepared is obtained spirulina through centrifugation, filtering, decompression, concentration and after drying respectively
95wt% ethanol extracts, spirulina 55wt% ethanol extracts, spirulina water extract and spirulina enzymolysis thing, i.e.,：Spiral
Algae full agonist.
Extraction solvent and the envelope-bulk to weight ratio for extracting material in step A-C（v:w）It is 30:1.
Embodiment 3
A kind of preparation method of spirulina full agonist, with spirulina as raw material, using opposed polarity（From small to large）It is molten
Agent extraction prepares spirulina full agonist：
First extracted with 90wt% ethanol, 90wt% ethanol is then extracted into residue 60wt% ethanol and is extracted, then by 60wt% second
Alcohol extracting residue water extraction, finally by water extraction residue digest, the extract solution that above-mentioned alcohol extracting, water extraction and enzymolysis are obtained respectively pass through from
The heart, filtering, decompression, concentration and dry prepared spirulina 90wt% ethanol extracts, spirulina 60wt% ethanol extracts, spirulina
Water extract and spirulina enzymolysis thing, i.e.,：Spirulina full agonist；Specifically include following steps：
A. spirulina fine powder is first used into 90wt% ethanol ultrasound assisted extraction 1.5h in 55 DEG C, filtering obtains spirulina 90wt%
Alcohol extract；
B. the spirulina residue utilization 60wt% ethanol that the 90wt% ethanol that will be obtained is extracted is in 55 DEG C of ultrasound assisted extractions
1.5h, filtering obtains spirulina 60wt% alcohol extracts；
C. the spirulina residue utilization deionized water that the 60wt% ethanol that will be obtained again is extracted carries out ultrasound-microwave association at 85 DEG C
With extraction or ultrasound assisted extraction 1.5h, filtering obtains spirulina Aqueous extracts；
D. the spirulina water extraction residue utilization 1.5wt% compound proteases of acquisition are carried out into enzymolysis 1.5h at 50 DEG C again, is filtered
To obtain the enzyme-extracting solution of spirulina；
E. the extract solution for step A-D being prepared is obtained spirulina through centrifugation, filtering, decompression, concentration and after drying respectively
90wt% ethanol extracts, spirulina 60wt% ethanol extracts, spirulina water extract and spirulina enzymolysis thing, i.e.,：Spiral
Algae full agonist.
Extraction solvent and the envelope-bulk to weight ratio for extracting material in step A-C（v:w）It is 25:1.
Application examples 1
Spirulina 95wt% ethanol in spirulina full agonist obtained by Example 2 is extracted, spirulina 55wt% ethanol
Extract, with oat, chlorella, gingko flavone extractive, milk, citric acid, xylitol and cycloheptaamylose
Compounding, is obtained a kind of auxiliary hyperglycemic reducing blood lipid, the nutritious spirulina oatmeal of liver protection；The raw material composition of the nutrient oatmeal
It is calculated by weight as：It is 5 parts of spirulina 95wt% ethanol extracts, 5 parts of spirulina 55wt% ethanol extracts, 10 parts of oat, small
5 parts of 2 parts of ball algae, 2 parts of gingko flavone extractive, 2 parts of milk, 2 parts of citric acid, 2 parts of xylitol and cycloheptaamylose.
Application examples 2
Spirulina full agonist and citric acid, xylitol and cycloheptaamylose compounding obtained by Example 2, are obtained one kind
Auxiliary hyperglycemic reducing blood lipid and the spirulina health-care oral liquid of liver protection；The raw material composition of the health care oral liquid is counted by weight
For：5 parts of spirulina 95wt% ethanol extracts, 5 parts of spirulina 55wt% ethanol extracts, 5 parts of spirulina water extract, spirulina
5 parts of 5 parts of zymolyte, 2 parts of citric acid, 2 parts of xylitol and cycloheptaamylose.
Experiment test
Spirulina opposed polarity（Full agonist）Influence to high fat diet inducing obesity rat blood index
Experimental technique：
To meet modern humans' life style（High fat diet custom based on animal food）Angle set out, it is high using sugar high
Fat feed（Basal feed 67%, lard 10%, sucrose 20%, cholesterol 3%）Set up the glucose -lipid metabolism disorder mould of high fat diet induction
Type, carries out the Dietary frequency feeding trial of 2 months by a definite date.Experiment sets 6 groups, including Normal group（NFD）, fat control group high
（HFD）, the ethanol extract group of spirulina 95%（SPL95）, the ethanol extract group of spirulina 95%（SPL55）, spirulina water extract
Thing group（SPWE）, spirulina water extract residue enzymolysis（SPEM）And mixture group（SPMX）.After giving given the test agent 60 days,
Fasting Wistar rats are anaesthetized, in clean bench, the apex of the heart is first passed through and is taken blood, by blood collection in heparin tube, passed through
T-CHOL (TC), triglyceride (TG), HDL-C in automatic biochemistry analyzer and kit assay serum
(HDL-C), the LDL-C (level such as LDL-C.
Test result indicate that：Spirulina opposed polarity extract, compound protease zymolyte and full agonist have aobvious
The auxiliary effect for reducing blood fat of work（Fig. 1）, wherein through experimental group（SPL95、SPL55、SPWE、SPEM、SPMX）The fat high drink of gavage
The physiochemical indice for eating inducing obesity rat is changed with blank group compared with model group, wherein in can significantly reducing serum
T-CHOL（TC）, triglyceride (TG) and LDL-C (LDL-C) level, make HDL courage
The level of sterol (HDL-C) increases.
The foregoing is only presently preferred embodiments of the present invention, all impartial changes done according to scope of the present invention patent with
Modification, should all belong to covering scope of the invention.
Claims (5)
1. a kind of preparation method of spirulina full agonist, it is characterised in that：With spirulina as raw material, using polarity from it is small to
Big solvent is extracted successively, and finally water extraction residue is digested with compound protease, prepares spirulina full agonist：First use
85wt%-95wt% ethanol is extracted, and 85wt%-95wt% ethanol then is extracted into residue 55wt%-65wt% ethanol and is extracted, then
55wt%-65wt% ethanol is extracted into residue water extraction, finally water extraction residue is digested, above-mentioned alcohol extracting, water extraction and enzymolysis are obtained
Extract solution through centrifugation, filtering, decompression, concentration and dry prepared spirulina 85wt%-95wt% ethanol extracts, spirulina respectively
55wt%-65wt% ethanol extracts, spirulina water extract and spirulina enzymolysis thing, i.e.,：Spirulina full agonist.
2. the preparation method of a kind of spirulina full agonist according to claim 1, it is characterised in that：The spirulina
The preparation method of full agonist specifically includes following steps：
A. spirulina fine powder is first used into 85wt%-95wt% ethanol microwave and ultrasonic wave synergic extraction or ultrasonic wave added in 45-70 DEG C
0.5-3h is extracted, filtering obtains spirulina 85wt%-95wt% alcohol extracts；
B. the spirulina residue utilization 55wt%-65wt% ethanol that the 85wt%-95wt% ethanol that will be obtained is extracted is at 45-70 DEG C
Microwave and ultrasonic wave synergic extraction or ultrasound assisted extraction 0.5-3h are carried out, filtering obtains spirulina 55wt%-65wt% alcohol extracts；
C. the spirulina residue utilization deionized water that the 55wt%-65wt% ethanol that will be obtained again is extracted is carried out at 70-100 DEG C
Microwave and ultrasonic wave synergic extraction or ultrasound assisted extraction 0.5-3h, filtering obtain spirulina Aqueous extracts；
D. the spirulina water extraction residue utilization compound protease of acquisition is carried out into enzymolysis 0.5-3h at 37-65 DEG C again, filtering with
Obtain the enzyme-extracting solution of spirulina；
E. the extract solution for step A-D being prepared is obtained spirulina through centrifugation, filtering, decompression, concentration and after drying respectively
85wt%-95wt% ethanol extracts, spirulina 55wt%-65wt% ethanol extracts, spirulina water extract and spirulina enzyme
Solution thing, i.e.,：Spirulina full agonist.
3. the preparation method of a kind of spirulina full agonist according to claim 2, it is characterised in that：In step A-C
Extraction solvent is 10 with the envelope-bulk to weight ratio for extracting material:1-30:1.
4. the preparation method of a kind of spirulina full agonist according to claim 2, it is characterised in that：In step D
The compound protease addition is the 0.2-2wt% of spirulina water extraction residue.
5. the application of spirulina full agonist obtained in a kind of preparation method as claimed in claim 1, it is characterised in that：Take
One or more active material compoundings for having identical effect with other in obtained spirulina full agonist are prepared such as
Oral liquid, capsule, tablet, electuary, the nutrient functional foods of the different dosage forms of solid beverage.
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| CN109329925A (en) * | 2018-12-07 | 2019-02-15 | 丽江程海湖天然螺旋藻生产基地有限公司 | A kind of spiral algae sheet and preparation method thereof being adjustable body immunity |
| CN109329901A (en) * | 2018-12-10 | 2019-02-15 | 丽江程海湖天然螺旋藻生产基地有限公司 | A kind of lucid ganoderma spirulina tablet of strengthen immunity and preparation method thereof |
| CN109393474A (en) * | 2018-12-06 | 2019-03-01 | 丽江程海湖天然螺旋藻生产基地有限公司 | A kind of selenium enriched Spirulina piece and preparation method thereof enhanced human immunity |
| CN109734761A (en) * | 2019-02-28 | 2019-05-10 | 福建农林大学 | A kind of preparation method and applications of Enteromorpha fucose complex compound |
| CN113543793A (en) * | 2019-03-05 | 2021-10-22 | 韩国海洋科学技术院 | Preparation method of spirulina extract, and pharmaceutical composition and health functional food containing spirulina extract for improving cognitive ability |
| CN114392222A (en) * | 2021-12-21 | 2022-04-26 | 上海瑞帝安生物科技有限公司 | Gradient extraction process of plants and application thereof |
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