CN104814423A - An extraction method for low-sodium chloride and high-glutamic acid plant-salt from moderate salinization suaeda salsa - Google Patents
An extraction method for low-sodium chloride and high-glutamic acid plant-salt from moderate salinization suaeda salsa Download PDFInfo
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- CN104814423A CN104814423A CN201510210965.8A CN201510210965A CN104814423A CN 104814423 A CN104814423 A CN 104814423A CN 201510210965 A CN201510210965 A CN 201510210965A CN 104814423 A CN104814423 A CN 104814423A
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Abstract
The present invention provides an extraction method for low-sodium chloride and high-glutamic acid plant-salt from moderate salinization suaeda salsa, and includes suaeda salsa cultivation and plant-salt extraction; the plant-salt extraction comprises washing and subcritical liquid ammonia extracting. The suaeda salsa is high in emergence rate and transplanting survival rate; in mid-October, the suaeda salsa has a plant density of 500-510 plants/m2, an average biomass of 980-1100 g/m2 and a cover degree of 96-97%; the method can effectively improve saline-alkali soil of planting areas, reduce soil conductivity, and reduce soil salinity; the content of sodium chloride in the prepared plant-salt is 22.7-22.9% and the content of potassium chloride in the prepared plant-salt is 13.3-13.6% the prepared plant-salt has balanced nutrition indexes and significantly increased contents of aspartic acid, arginine, lysine, glutamic acid, glycine.
Description
Technical field
The present invention relates to a kind of extracting method of plant salt, be specifically related to the extracting method of a kind of moderate salinization Suaeda salsa low sodium homoamino acid plant salt, belong to alkaline land improvement and field of comprehensive utilization.
Background technology
Relate to the artificial cultivation carrying out salt-tolerant plant in salt-soda soil in prior art, but its agricultural use being mainly used in saline-alkali environment with products such as harvested vegetable oil plants for main purpose, effective to alkaline land improving effect, and the technique extracting plant salt is immature.There is following shortcoming in the planting technologies such as management of sowing that prior art is taked: 1, seed is easily blown away wholely, affects the foundation of vegetation; 2, the sowing time is on the occasion of arid season, and rainfall is rare, cannot irrigate again, affect seed germination; Therefore the growth of seed is all affected.
From the method for the fluffy middle extraction plant salt of alkali in prior art, technique is simple, and plant salt yield is low, and impurity is many, cannot meet people for plant salt nutrition, safe, healthy requirement.
Existing plantation alkali is fluffy and have the following disadvantages from the technology of the fluffy middle extraction plant salt of alkali: alkaline land soil electrical conductivity is high, and salinity is high; During the fluffy plantation of alkali, transplanting survival rate is low; From the plant salt of alkali fluffy middle extraction, amino acid content is low.
Summary of the invention
The present invention is the deficiency solving prior art existence, provides the extracting method of a kind of moderate salinization Suaeda salsa low sodium homoamino acid plant salt, to realize following goal of the invention:
(1) the nursery stage, the fluffy emergence rate of alkali is high, and after transplant planting, survival rate is high, mid-October, and plant spacing reaches 500-510 strain/m2, and average biomass reaches 980-1100g/ m
2, cover degree reaches 96-97%.
(2) effectively improve the alkaline land soil of planting area, reduce soil conductivity, reduce Soil salinity.
(3) in the plant salt prepared, the content of sodium chloride is 22.7-22.9%, and the content of potassium chloride is 13.3-13.6%.
(4) in the plant salt prepared, every nutritive index is balanced, and the content of aspartic acid, arginine, lysine, glutamic acid, glycine significantly improves.
For solving above technical problem, technical scheme provided by the invention is as follows:
An extracting method for moderate salinization Suaeda salsa low sodium homoamino acid plant salt, the plantation and the plant salt that comprise Suaeda salsa extract; Described plant salt extracts, and comprises cleaning, the extraction of subcritical liquefied ammonia.
Below the further improvement to technique scheme:
Described subcritical liquefied ammonia extraction, extraction time is 0.5-3 hour.
Described subcritical liquefied ammonia extraction, extraction temperature is 18 DEG C ~ 44 DEG C.
Described subcritical liquefied ammonia extraction, extracting pressure 0.7 MPa ~ 2.6MPa.
Described salt-soda soil, be moderate salty soils plot, 0-30cm soil layer salt content is 1.0-1.4%, pH is 7.6-7.8.
Described cleaning, the flow velocity of water remains on 11-15ml/s, and water temperature controls at 0-10 DEG C.
Beneficial effect of the present invention is:
(1) alkali of the present invention is fluffy in the nursery stage, and the fluffy emergence rate of alkali is 99.2-99.6%, and after transplant planting, survival rate is 96-98%, mid-October, and plant spacing is 500-510 strain/m
2, average biomass is 980-1100g/ m
2, cover degree is 96-97%.
(2) the present invention effectively improves the alkaline land soil of planting area, reduces soil conductivity, and reduce Soil salinity, electrical conductivity is 1.86-1.93, and Soil salinity is 0.16-0.2%.
(3) in the plant salt prepared of the present invention, sodium chloride content is 22.7-22.9%, and KCE content is 13.3-13.6%.
(4) in the plant salt that prepared by the present invention, amino acid whose content is significantly improved, aspartic acid is 25.3-25.5 mg/g, and arginine is 19.6-19.8 mg/g, and lysine is 15.9-16.0 mg/g, glutamic acid is 35.5-35.9 mg/g, and glycine is 15.2-15.5 mg/g.
(5) in the plant salt prepared of the present invention, the content of vitamin is significantly improved, and VC is 42.0-42.5mg/g, VB1 be 28.1-28.3 mg/g, VB2 be 29.8-30.0 mg/g, VB6 is 36.8-40.0 mg/g.
Detailed description of the invention
Embodiment 1
An extracting method for moderate salinization Suaeda salsa low sodium homoamino acid plant salt, comprises the following steps:
1, the plantation of Suaeda salsa
1) selection in salt-soda soil
Select 0-30cm soil layer salt content to be 1.0-1.4%, pH to be the moderate salty soils plot of 7.6-7.8.
2) soil is arranged
Carry out before sowing ploughing, harrowing operation, make topsoil smooth in small, broken bits, weeds Ex-all, every furrow are wide 3 meters, and furrow are smooth, work furrow width 35cm, ditch depth 12cm, and the wide 45cm of ring field ditch, dark 35cm can be advisable water discharge in time.
3) Seed Treatment
Preparation root-inducing powder, seed soaking
Brassin lactones 0.05 ~ 0.1 part, S-abscisic acid: 0. 5-1 part, salicylic acid 5-7 part, biochemical fulvic acid: 15-25 part.
Get above-mentioned root-inducing powder 0.7g ~ 1g first to dissolve with a small amount of alcohol, then add 1L water, seed soaking time 1-2h.
Seed pelleting
Paenibacillus polymyxa, bacillus amyloliquefaciens, bacillus licheniformis, microbial streptomycete microbial inoculum according to: the ratio of 1-10:1-10:5-10:5-10 joins in sticker, after stirring, add seed again, repeatedly stir, make each seed is all stained with microbial inoculum, the mass ratio of seed and microbial inoculum is 1:5-10.Wherein, the sticker of use be preferably selected from pectin, guar gum, carragheen, propylene glycol alginate, xanthans one kind or two or more.
Seed puts into seed-coating machine, repeatedly shakes mixing through seed-coating machine, makes each seed all wrap one deck coating material, finally seed is put in ventilation, dry in the shade stand-by.Wherein, what described coating material was preferably selected from white carbon, bentonite, diatomite is one kind or two or more.
4) nursery
Matrix group becomes maize pulp 10-15 part, stalk 15-20 part, peat composed of rotten mosses 27-30 part, perlite 10-15 part, plant ash 16-20 part, matrix particle size range is 130-150 order, matrix salinity is 0.7-2.0%, water content 51-55%, total porosity 60-63%, in butt matter, the content of organic matter is 17-18%, N+P
2o
5+ K
2o total nutrient content 6-8%, EC value is 1.1-1.2mS/cm, pH is 7.3-7.6; Seedbed is made up of multiple seedling culture hole plate, and each seedling culture hole plate evenly offers multiple hole, and the seed after vernalization process of learning from else's experience is program request 2-3 grain in the hole of seedling culture hole plate, earthing 3-5mm, and the density of cultivation is 250 strains/m
2, stable temperature of sprouting is 15.5-16 DEG C.
Seedling culture hole plate specification is 40cm × 30cm × 5cm, and on each seedling culture hole plate, the quantity in hole is 40, and the specification in hole is 5cm × 5cm × 5cm.
In the seedbed planting process of seed, on seedling culture hole plate, after planting cover support film or seedling culture hole plate is moved into seedling rearing room, to ensure temperature of sprouting.
Described maize pulp, stalk are through the thing that becomes thoroughly decomposed of fermentation process, described fermentation process carries out decomposition reactor system process with complex microbial inoculum: by maize pulp, straw heap, adjustment water content is 61-64%, cover film, carry out pre fermentation 1 day, carry out aerobic fermentation afterwards: when heap temperature reaches more than 60 DEG C, every turning in 2-3 days successively, when heap temperature is reduced to below 50 DEG C, every turning in 7 days successively, fermentation about 1 month, when heap temperature is stabilized in close to environment temperature, fermentation completely.The described fermentation process time is 1 month; Complex microbial inoculum comprises and mixing the strong bacillus of cellulose-decomposing ability, actinomyces, filamentous fungi.
5) field planting, field management
Early seedling stage manages
The fluffy seedling of alkali in field planting mid-April, with brackish water (water salinity is 1 ~ 2g/l, and pH value is 7.3 ~ 7.5, and sodium adsorption ratio is 2.0 ~ 2.1) pouring once, sprinkle water twice daily afterwards, the globule can not be too large and water outlet is too violent; After 30 days, adopt flood irrigation, once a day, the dark 2cm of the furrow water surface is advisable, and water emits after can stopping one hour.
Fertilising in after planting 3 weeks;
Middle and later periods management
A, seedling grow to 30 days, shoot root prick dark after, adopt flooding irrigation method, 2 days are once, keep ground moistening, ponding should be drained after raining, and allow the certain salinity of soil conservation.
B, planting density, keep every square metre has 180-190, and density is excessive, should dredge early and moves or mend cultivation, keep certain planting density in seedling stage.
C, 80 days, enter florescence, according to glasswort growing state, be improve disease and insect resistance, ability resistant to lodging, timely application of organic fertilizers or chemical fertilizer.
2, plant salt extracts
1) clean
Select Suaeda salsa young stem and leaf, cob edible portion, segment, length is 5cm, and 10 sections is one group;
Regulate the water yield of water for cleaning, keep flow velocity to linear, the flow velocity of water remains on 11-15ml/s, and water temperature controls at 0-10 DEG C, gets out the brushing tool that hair is made;
Fluffy for the alkali of segment cauline leaf is placed in forefinger, is soaked by brushing tool, the train of thought along cauline leaf is scrubbed gently, and till clean, and the cauline leaf after clean is placed in screen cloth pallet drains away the water, often organizing scavenging period is 5-7 minute;
Fluffy for the alkali of segment cob is placed in the centre of the palm, scrubs gently, brush limit, limit rotates, and clean be placed on screen cloth pallet and drain away the water.Often organizing scavenging period is 10-11 minute.
2) dry
Be put in basin by fluffy for the alkali cleaned up, the thickness that in dish, alkali is fluffy is 2-3cm, be placed in heated-air circulation oven, baking temperature controls at 50-60 DEG C, baking temperature preferably 50 DEG C, drying time is 3-5 hour, and after dry, moisture is no more than the 9-12% of the fluffy gross mass of alkali, naturally cool to room temperature, obtain dry alkali fluffy.
3) pulverize
Coarse crushing: dried alkali is fluffy, is cut into the section of 7-8mm size, uses pulverizer coarse crushing, obtains meal, and particle diameter is 300 μm-400 μm.
Air-flow crushing: arranging reservoir pressure is 0.7-1MPa; Screw feed speed is 20hz; The set of frequency of grader motor is 13hz, and the particle diameter of crushing rear material is 150-200 μm.
4) ball milling broken wall
By above-mentioned fine powder, add ball mill, carry out ball milling broken wall, drum's speed of rotation is 40-50 rev/min, through ball mill grinding in 0.5-3 hour, obtains the Ultramicro-powder that particle diameter is 20-100 μm.
5) subcritical liquefied ammonia extraction
Material is sent in the extractor of construction of pressure vessel, after closing feeding-passage, Pressure Drop extremely-0.09MPa in device is made after deflating, squeeze into liquefied ammonia extractant and carry out three counter-current extractions, extraction time is 0.5-3 hour, extraction temperature is 18 DEG C ~ 44 DEG C, extracting pressure 0.7 MPa ~ 2.6MPa; Liquefied ammonia flow is 4-9L/h, containing 6% entrainer in liquefied ammonia, comprise 65% ethanol, ethyl acetate 25%, butanols 10%, primary separator pressure 0.3-0.6MPa, temperature is 32-42 DEG C, second-stage separator pressure is 0.1-0.3 MPa, temperature is 31-44 DEG C, and the extract of extraction gained, enters vapo(u)rization system, solvent is gasified be separated with extract, after having extracted, exhaust liquid flux in extractor, open the air outlet valve of extractor, the gasification of the liquefied ammonia solvent making to adsorb in material, entrainer respectively with feed separation, discharge extractor.The liquefied ammonia gas evaporated from material and in extract, compress and liquefy through ammonia compressor, flow back to solvent circulating tank, recycle, entrainer also recycles.
6) extract pressed powder is prepared
Collect extract, add the ethyl acetate of 50%, stirring and dissolving, then add the superfine silica gel powder of 50%, be uniformly mixed, crushed 100 eye mesh screens, mix, and at 40 DEG C, volatilization removing ethyl acetate, is extracted thing pressed powder.
7) vacuum freeze drying
Sublimation drying is 5-13 hour, and cryogenic temperature is-38 ~-52 DEG C, and hothouse vacuum pressure is 3-12Pa, and vacuum freeze drying is less than 2% to containing water gaging.
8) pulverize, seal and preserve
Being crushed to particle diameter is 10-15 μm, and sealing is preserved.
Embodiment 2
An extracting method for moderate salinization Suaeda salsa low sodium homoamino acid plant salt, comprises the following steps:
1, the plantation of Suaeda salsa
1) selection in salt-soda soil
2) soil is arranged
3) Seed Treatment
Above-mentioned steps operates according to the method for embodiment 1, and the parameter of change is in table 1.
Table 1
4) nursery
Carry out nursery according to the method for embodiment 1, change parameter in table 2.
Table 2
5) field planting, field management
Operate according to the method for embodiment 1.
2, plant salt extracts
Carry out the extraction of plant salt according to the mode of embodiment 1, the parameter of change is in table 3.
Table 3
Result of the test is as follows:
In the nursery stage, the fluffy emergence rate of alkali is 99.2-99.6%, and after transplant planting, survival rate is 96-98%, mid-October, and plant spacing is 500-510 strain/m
2, average biomass is 980-1100g/ m
2, cover degree is 96-97%.The present invention effectively improves the alkaline land soil of planting area, reduces soil conductivity, and reduce Soil salinity, concrete effect is in table 4.
Every nutritive index in the fluffy middle extraction plant salt of alkali effectively improves, and has the feature of low sodium, and more healthy, specific targets are in table 5.
Table 4
Table 5
The above is the citing of best mode for carrying out the invention, and the part wherein do not addressed in detail is the common practise of those of ordinary skill in the art.Protection scope of the present invention is as the criterion with the content of claim, and any equivalent transformation carried out based on technology enlightenment of the present invention, also within protection scope of the present invention.
Claims (6)
1. an extracting method for moderate salinization Suaeda salsa low sodium homoamino acid plant salt, is characterized in that: the plantation and the plant salt that comprise Suaeda salsa extract; Described plant salt extracts, and comprises cleaning, the extraction of subcritical liquefied ammonia.
2. the extracting method of a kind of moderate salinization Suaeda salsa according to claim 1 low sodium homoamino acid plant salt, is characterized in that: described subcritical liquefied ammonia extraction, extraction time is 0.5-3 hour.
3. the extracting method of a kind of moderate salinization Suaeda salsa according to claim 1 low sodium homoamino acid plant salt, is characterized in that: described subcritical liquefied ammonia extraction, extraction temperature is 18 DEG C ~ 44 DEG C.
4. the extracting method of a kind of moderate salinization Suaeda salsa according to claim 1 low sodium homoamino acid plant salt, is characterized in that: described subcritical liquefied ammonia extraction, extracting pressure 0.7 MPa ~ 2.6MPa.
5. the extracting method of a kind of moderate salinization Suaeda salsa according to claim 1 low sodium homoamino acid plant salt, it is characterized in that: the plantation of described Suaeda salsa comprises the selection in salt-soda soil, described salt-soda soil is moderate salty soils plot, 0-30cm soil layer salt content is 1.0-1.4%, pH is 7.6-7.8.
6. the extracting method of a kind of moderate salinization Suaeda salsa according to claim 1 low sodium homoamino acid plant salt, it is characterized in that: described cleaning, the flow velocity of water remains on 11-15ml/s, and water temperature controls at 0-10 DEG C.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105901643A (en) * | 2016-04-19 | 2016-08-31 | 王胜 | A plant salt and a method of extracting the plant salt high in amino acids from suaeda salsa by adopting compound proteases |
| CN105901644A (en) * | 2016-04-19 | 2016-08-31 | 王胜 | A plant salt containing astragaloside and a preparing method thereof |
| CN105901641A (en) * | 2016-04-19 | 2016-08-31 | 王胜 | A plant salt and a method of extracting the plant salt from suaeda salsa by adopting an ultrasonic-assisted enzymolysis process |
| CN105919023A (en) * | 2016-05-03 | 2016-09-07 | 王胜 | Plant salt containing celery extract, and preparation method of plant salt |
| CN105942433A (en) * | 2016-05-03 | 2016-09-21 | 王胜 | Plant salt containing pine pollen extracts and preparation method thereof |
| CN109168860A (en) * | 2018-08-24 | 2019-01-11 | 江苏省农业科学院 | A kind of implantation methods of northern Suzhou Huanghai Sea salt-soda soil tree |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105901643A (en) * | 2016-04-19 | 2016-08-31 | 王胜 | A plant salt and a method of extracting the plant salt high in amino acids from suaeda salsa by adopting compound proteases |
| CN105901644A (en) * | 2016-04-19 | 2016-08-31 | 王胜 | A plant salt containing astragaloside and a preparing method thereof |
| CN105901641A (en) * | 2016-04-19 | 2016-08-31 | 王胜 | A plant salt and a method of extracting the plant salt from suaeda salsa by adopting an ultrasonic-assisted enzymolysis process |
| CN105919023A (en) * | 2016-05-03 | 2016-09-07 | 王胜 | Plant salt containing celery extract, and preparation method of plant salt |
| CN105942433A (en) * | 2016-05-03 | 2016-09-21 | 王胜 | Plant salt containing pine pollen extracts and preparation method thereof |
| CN109168860A (en) * | 2018-08-24 | 2019-01-11 | 江苏省农业科学院 | A kind of implantation methods of northern Suzhou Huanghai Sea salt-soda soil tree |
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