CN104807669A - Feces liver fluke egg collection and separation equipment and method - Google Patents
Feces liver fluke egg collection and separation equipment and method Download PDFInfo
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- CN104807669A CN104807669A CN201510262936.6A CN201510262936A CN104807669A CN 104807669 A CN104807669 A CN 104807669A CN 201510262936 A CN201510262936 A CN 201510262936A CN 104807669 A CN104807669 A CN 104807669A
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Abstract
The invention provides feces liver fluke egg collection and separation equipment. The collection and separation equipment comprises a sampling device and a separating device, wherein the sampling device comprises a sample cover, a sampling rod and a cylindrical sampling head, the separating device comprises a sample bottle, a sealing plug and a filter mesh, the sample cover is arranged on a bottle mouth of the sample bottle in a covering manner so as to enable the sampling rod and the sampling head to be located in the sample bottle, the sample bottle is divided into an upper segment and a lower segment, the upper segment serves as a sample chamber, the lower segment serves as a separation chamber, the separation chamber is conical, the sealing plug is arranged at the conical end part of the separation chamber, and the filter mesh is arranged in the separation chamber. The embodiments of the invention further provide a feces liver fluke egg collection and separation method which comprises the steps: sampling feces with the sampling device, then, putting the feces sample into the sample bottle; tightly sealing with the sealing plug, then, opening the sample cover, pouring liquid to submerge the sampling head, covering the sample cover, vertically placing the sample bottle, and standing so as to obtain a sample to be tested; loosening the sealing plug, collecting the sample to be tested, and carrying out quantitative detection. The collection and separation equipment and the method have the advantages that standardized operation is realized, and quantitative detection can be realized.
Description
Technical field
The present invention relates to clinical treatment and prevention and control of diseases field, especially relate to a kind of ight soil clonorchis egg and gather separation equipment and method.
Background technology
Clinical treatment and prevention and control of diseases are very arduous and tasks of shouldering heavy responsibilities always, and its mission is by the diagnosis and treatment of disease, deformity and injury and prevention and control, creates health environment, maintains social stability, safeguard national security, promote people ' s health; Its aim is take scientific research as support, it be basic by the talent, centered by disease prevention and cure, defending under planning commission leader, play technical management and technical service function, around national disease prevention and cure key task, strengthen the research to disease treatment and preventative strategies can and measure, carry out the organizing and implementing of various diseases preventing and controlling planning.
In order to realize clinical treatment and disease prevention and cure target, from human excrement and urine's excreta, finding liver fluke worm's ovum, realizing that aetology is carried out to this Disease and make a definite diagnosis and carry out the object of disease popularity district field monitoring.
By the end of at present, check that the method for liver fluke in ight soil [mainly comprising: clonorchis sinensis (Clonorchis sinensis), top fan trough (Haplorchis taichui), Metagonimus Yokogawai (Metagonimus yokogawai), haplorchis pumilio (Haplorchispumilio), centrocestus cuspidatus (Centrocestus formosanus), Metorchis taiwanensis (Metorchis taiwanensis) and armful stem sour jujube gap are inhaled (Echinochasmus perfoliatus) etc.] worm's ovum mainly contains three kinds.(1) method of direct smear; (2) aldehyde ether centrifugal; (3) Kato-Katz method.Method of direct smear is simple to operate, and instrument equipment is also fairly simple, and concrete operation step is: drip physiological saline in the microslide of cleaning, with the ight soil block of cotton swab rod or toothpick picking mung bean size, smears evenly in physiological saline; Through painting agreement that contracts a film or TV play to an actor or actress, the thickness of smear is can recognize that the writing on book is advisable.Generally check under low power lens, as observed with high power lens, need with cover plate.The discriminating of foreign matter in worm's ovum and ight soil should be noted.Worm's ovum all has definite shape and size; Chorion smooth surface is neat, the intrinsic color and luster of tool; Ovum includes egg cell or larva.But this method recall rate is low, easily undetected and can only reach the degree of qualitative examination, egg counts roughly can only lean on visual inspection, cannot realize objective evaluation.
Its concrete operation step of second method aldehyde ether centrifugal is: get 1-2g sample in container, the 10-20ml that adds water mixes well, ight soil suspension is filtered through 2 layers of gauze (or 100 order metallic sieves), centrifugal (2000rpm/min) 2 minutes; Remove upper strata liquid manure, retain sediment, the 10ml that adds water mixes, centrifugal 2min; Remove liquid, add 10% formaldehyde 7ml.Added diethyl ether after 5 minutes 3ml, and the jam-pack mouth of pipe also fully shakes up, and takes off mouth of pipe plug, more centrifugal 2min, is namely divided into 4 layers from bottom to top in visible pipe.Get sediment smear for microscopic examination at the bottom of pipe.As checked protozoon packing, Iodine staining can be added, with cover plate microscopy.The comparatively front a kind of method precision of this method is many, and improves recall rate, but its auxiliary material needed are many, but also needs to use this hazardous chemical of ether, and step operates also more complicated.
The third method Kato-Katz method is the snail fever quantitative detecting method that current WHO recommends, and it checks that excrement amount is only 41.7mg less, can realize quantitative detection.But feces collection and follow-up sample disposal process depart from, and period needs artificial treatment stool sample, adds work difficulty and workload.And Kato-Katz method due to sampling amount few, the representativeness of sample is poor, also has considerable influence to the accuracy of testing result.
In view of this, this invention of special proposition.
The method of three kinds of inspection ight soil clonorchis eggs of the prior art, all exist and cannot realize normalizing operation, unified standard operating process that can not be for reference, gather and subsequent sample process detachment process disengaging operation, reduce work efficiency, in order to solve the technical matters of above appearance, the present invention has invented a kind of ight soil clonorchis egg and has gathered separation equipment and gather separation method.
The ight soil clonorchis egg that the embodiment of the present invention provides gathers separation equipment, stool collection and subsequent sample process detachment process are integrated, apparatus design is integral type, simplicity of design, do not need additionally to prepare other equipment, sample handling processes is totally-enclosed, clean, efficiently, normalizing operation can be realized, quality control is easier, its equipment set carries out lock out operation for the clonorchis egg in ight soil specially, and other ight soil residues and worm's ovum of curing the disease cannot finally enter the lowermost end of split cavity by sieve aperture and filter screen, the diameter of visible sieve aperture and the mesh order number of filter screen are all by well-designed and numerical range that is that finally determine.
Further, after preventing gathering ight soil, dropping of ight soil residue, the diameter of its sieve aperture is 1.3-1.5mm, and between sieve aperture, spacing is 1-1.2mm.
Further, the material of described filter screen is metal, is preferably stainless steel; Described filter screen is layer 2-3, is from top to bottom uniformly distributed in described split cavity.
Further, described filter screen is 2 layers, and upper screen is dome-shaped, is arranged at described split cavity one end near described sample chamber, and mesh order number is 100-150 order, and lower screen arranges near described sealing plug and is straight tabular, and mesh order number is 180-200 order.Upper screen in split cavity is that dome-shaped can increase contact area, allows clonorchis egg pass through and stops ight soil residue, and the filter screen of bottom then plays the effect of filtering further.
Further, described sample bottle is cylindric and diameter is 3-4cm, and the height of described sample chamber is 8-9cm, and the height of described split cavity is 4-5cm; Described sampling head is cylindric, its diameter be highly 8-9mm.
Further, the bottom of described sealing plug is concordant with the bottom of described sample bottle, makes concordant shape like this and is more conducive to placing.
The embodiment of the present invention additionally provides and utilizes above-mentioned ight soil clonorchis egg collection separation equipment to carry out gathering the method be separated, and comprises the steps:
(A), after first adopting described sampler to take ight soil 0.3-0.35g, described sample bottle is put into inner;
(B) sample lid is opened after being tamping by sealing plug, pour liquid into and do not have sample lid on described sampling head bonnet, vertical placement 30-50min is also with sample bottle described in certain interval of time horizontal jitter, and finally standing 3-5min obtains the testing sample containing clonorchis egg;
(C) loosen sealing plug, collect all testing samples and quantitatively detect.
The ight soil clonorchis egg that the embodiment of the present invention provides gathers separation method, sample collection can realize quantitative collection, generally be designed to 0.3-0.35g, quantitative concrete numerical value is by after considering that concrete specification is designed in the convenient and sampling accuracy of sampling when design sample head, determine sampling amount again, thus it is few to overcome Kato-Katz method sampling amount, the representativeness of sample is poor, testing result accuracy is had to the technical matters of considerable influence, because sampling amount is obviously more than Kato-Katz method, the theoretical accuracy of testing result is also higher than the former.When being separated after gathering ight soil, detect reagent and directly use distilled water, avoid the generation adopting chemical reagent contaminated environment in the past, operator's health is also had to the phenomenon of harm, sampling process is simple, convenient, clean, can be completed voluntarily by person under inspection, not need professional to operate.The detachment process of sample make use of clonorchis egg proportion and is greater than water, can the principle of natural sedimentation, is separated by filter screen.
Further, when adopting described sampler to gather ight soil, sampling head is all immersed in ight soil to make described sampling head be filled up ight soil.Can be sampled voluntarily by patient during sampling operation, but also sampling head can be utilized to carry out ight soil mixing, enhance the representativeness of sample.
Further, in described step (A), when adopting described sampler to gather ight soil, sampling head is all immersed in ight soil to make described sampling head be filled up ight soil.
Further, in described step (B), every 5-10min horizontal jitter sample bottle in vertical put procedure.
Further, the time of each horizontal jitter sample bottle is 5-10s.Horizontal jitter is the mesh in order to prevent the ight soil residue fallen from blocking filter screen, also in order to accelerate the whereabouts filtering rate of clonorchis egg.Horizontal jitter in its standing separation process need operate gently, and does not steadily allow device to any direction over-tilting, prevents the worm's ovum that precipitated excessively floating again owing to rocking.
Further, in described step (B), liquid used is the buffer solution of pure water or PH7-7.5, and the volume pouring liquid into is the 75-80% of described sample bottle cumulative volume;
Further, in described step (C), the method quantitatively detected comprises: be collected in centrifuge tube by all testing samples, calculate the number of all clonorchis eggs after centrifugation.
Further, also can adopt natural sedimentation, but the time can be relatively long, quantitative and qualitative examination carries out all under the microscope.
In addition, ight soil clonorchis egg of the present invention gathers separation equipment when carrying out specific design, its optimal design specification is sample bottle overall height is 12cm, diameter is 3cm, wherein sample chamber is 8cm, split cavity is 4cm, cumulative volume is 55ml, distilled water dosage general control is at the 75-80% of sample bottle cumulative volume, namely be added to the distilled water of 40-45ml, dosage too much easily causes liquid splash contaminated environment also can lose sample, the very few mixing that can be unfavorable for again sample of dosage, therefore distilled water preferably controls suitable to add in weight range at one, ensure that the success be separated is with efficient.Most preferred configuration is: filter screen is two-layer, and be stainless steel, the mesh order number of upper screen is 100 orders, the mesh order number of lower screen is 200 orders, volume between upper screen to lower screen is 9ml, volume between lower screen to sealing plug is 400 μ L, and the volume at sealing plug place is 30 μ L.Thief rod length is 5cm, and diameter is 3cm, and sampling head diameter is 8mm, and be highly 8mm, sampling amount controls at 0.3g.In order to avoid reusing the experimental error of the false positive results of generation, be designed to single use device, one-piece construction is simple, processing ease.Except filter screen adopts stainless steel, miscellaneous part all can adopt plastic material, and cost is very low, is generally 2-3 unit, and one-piece construction is simple, processing ease.
Ight soil clonorchis egg of the present invention gathers separation method, compares, use this device separates clonorchis egg can realize closure process with " aldehyde-ether " method and Kato-Katz method etc., and operating process is simple, more clean.Worm's ovum collects rear detection, makes microscopy process easier, improves result accuracy.Stool sampling amount, more than Kato-Katz method, makes the accuracy of quantitative result higher than the former.
Summary of the invention
The first object of the present invention is to provide a kind of ight soil clonorchis egg to gather separation equipment, to solve the collection that exists in prior art and the technical matters being separated two operation stepss and being separated, affecting work efficiency and can not realizing quantitatively to detect.
The second object of the present invention is to provide a kind of ight soil clonorchis egg to gather separation method, numerous and diverse to solve the operation steps existed in prior art, cannot realize normalizing operation, cannot obtain the technical matters of the sample of relatively accurate amount accurately, easily.
Ight soil clonorchis egg provided by the invention gathers separation equipment, comprising: sampler and tripping device; Described sampler comprises sample lid, thief rod and columned sampling head, one end of described thief rod is fixedly connected with described sample lid, the other end connects described sampling head, and the upper and lower surface of described sampling head is provided with sieve aperture, and the diameter of described sieve aperture is 1.3-1.5mm; Described tripping device comprises sample bottle, sealing plug and filter screen, and the bottleneck that described sample lid is covered on described sample bottle makes described thief rod and sampling head be positioned at described sample bottle;
Described sample bottle is divided into two sections, epimere is the sample chamber for holding the sample in described sampling head, hypomere is the split cavity for separating of the clonorchis egg gone out in described sample, described split cavity is tapered, described sealing plug is arranged at the tapered end of split cavity, described filter screen is arranged in described split cavity, and the mesh order number of described filter screen is 100-200 order.
Accompanying drawing explanation
In order to be illustrated more clearly in the specific embodiment of the invention or technical scheme of the prior art, be briefly described to the accompanying drawing used required in embodiment or description of the prior art below, apparently, accompanying drawing in the following describes is some embodiments of the present invention, for those of ordinary skill in the art, under the prerequisite not paying creative work, other accompanying drawing can also be obtained according to these accompanying drawings.
Fig. 1 is the structure front elevation of the ight soil clonorchis egg collection separation equipment of the first embodiment of the present invention;
Fig. 2 is the structure front elevation of the ight soil clonorchis egg collection separation equipment of the second embodiment of the present invention;
Fig. 3 is the structure front elevation of the ight soil clonorchis egg collection separation equipment of the third embodiment of the present invention;
Fig. 4 is the structure front elevation of the ight soil clonorchis egg collection separation equipment of the present invention's the 4th kind of embodiment;
Fig. 5 is the sampler structure front elevation of the ight soil clonorchis egg collection separation equipment of the first embodiment of the present invention;
Fig. 6-8 is the sampling head structural drawing of the ight soil clonorchis egg collection separation equipment of the first embodiment of the present invention;
Reference numeral:
101-sampler; 102-tripping device; 103-sample lid;
104-thief rod; 105-sampling head; 106-sample bottle;
107-filter screen; 108-sealing plug; 109-sieve aperture;
110-sample chamber; 111-split cavity.
Embodiment
Be clearly and completely described technical scheme of the present invention below in conjunction with accompanying drawing, obviously, described embodiment is the present invention's part embodiment, instead of whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art, not making the every other embodiment obtained under creative work prerequisite, belong to the scope of protection of the invention.
In describing the invention, it should be noted that, orientation or the position relationship of the instruction such as term " " center ", " on ", D score, "left", "right", " vertically ", " level ", " interior ", " outward " they be based on orientation shown in the drawings or position relationship; be only the present invention for convenience of description and simplified characterization; instead of instruction or imply the device of indication or element must have specific orientation, with specific azimuth configuration and operation, therefore can not be interpreted as limitation of the present invention.In addition, term " first ", " second ", " the 3rd " only for describing object, and can not be interpreted as instruction or hint relative importance.
In describing the invention, it should be noted that, unless otherwise clearly defined and limited, term " installation ", " being connected ", " connection " should be interpreted broadly, and such as, can be fixedly connected with, also can be removably connect, or connect integratedly; Can be mechanical connection, also can be electrical connection; Can be directly be connected, also indirectly can be connected by intermediary, can be the connection of two element internals.For the ordinary skill in the art, concrete condition above-mentioned term concrete meaning in the present invention can be understood.
Ight soil clonorchis egg provided by the invention gathers separation equipment, comprising: sampler 101 and tripping device 102; Described sampler 101 comprises sample lid 103, thief rod 104 and columned sampling head 105, one end of described thief rod 104 is fixedly connected with described sample lid 103, the other end connects described sampling head 105, the upper and lower surface of described sampling head 105 is provided with sieve aperture 109, and the diameter of described sieve aperture 109 is 1.3-1.6mm; Described tripping device 102 comprises sample bottle 106, sealing plug 108 and filter screen 107, and the bottleneck that described sample lid 103 is covered on described sample bottle 106 makes described thief rod 104 and sampling head 105 be positioned at described sample bottle 106; Described sample bottle 106 is divided into two sections, epimere is the sample chamber 110 for holding the sample in described sampling head, hypomere is the split cavity 111 for separating of the clonorchis egg gone out in described sample, described split cavity 111 is tapered, described sealing plug 108 is arranged at the tapered end of split cavity 111, described filter screen 107 is arranged in described split cavity 111, and the mesh order number of described filter screen 107 is 100-200 order.
The ight soil clonorchis egg that the embodiment of the present invention provides gathers separation equipment, be made up of sampler and tripping device two parts, wherein thief rod is designed to the structure with sample lid one, sampling head design becomes cylindric, upper and lower surface is provided with sieve aperture, the diameter of its sieve aperture is generally at 1.3-1.6mm, thus restriction sampling volume and the free setting that can realize worm's ovum, after preventing gathering ight soil, dropping of ight soil residue, the diameter of its sieve aperture is more preferably 1.3-1.5mm, that optimum is 1.5mm, between sieve aperture, spacing is 1-1.2mm, spacing overstocked also can being unfavorable for intercepts, therefore spacing need control in suitable scope.Sampling head global design is small and exquisite, has taken into full account the ease for operation of stool sampling.
In the present invention, tripping device forms primarily of sample bottle 106, sealing plug 108 and filter screen 107, sample bottle divide into two sections, the sample chamber of epimere and the split cavity of hypomere taper, layer 2-3 filter screen is provided with in split cavity, be uniformly distributed in described split cavity from top to bottom, the design of taper more can be conducive to the merging collection of worm's ovum.The material of filter screen is preferably metal, in order to anticorrosion is more preferably stainless steel.As the preferred scheme of one, filter screen is designed to two-layer, upper screen is dome-shaped, be arranged at described split cavity one end near described sample chamber, mesh order number is 100-150 order, fully increases separating area and ensures that ight soil sediment does not block mesh, and lower screen is near described sealing plug setting and in straight tabular, mesh order number is 180-200 order, ensures that clonorchis egg is smoothly by being separated.Finally collect in the end of taper and be gathered in around sealing plug.The bottom of the sealing plug of whole ight soil clonorchis egg separation equipment is concordant with the bottom of sample bottle, can realize sitting in level ground enterprising line operate.Whole equipment externally gets in cylindric, and the split cavity design of taper is then the inside at sample bottle.The diameter of sieve aperture and the size of the mesh order number of filter screen all aim at and are separated clonorchis egg and design, be through a large amount of creative experiments finally to determine, therefore preferably control in suitable scope, the excessive or too small object all not reaching clonorchis egg in separating feces of numerical value.
Gather the size of separation equipment with regard to whole ight soil clonorchis egg, sample bottle is preferably cylindric and diameter is 3-4cm, and the height of described sample chamber is preferably 8-9cm, and the height of described split cavity is preferably 4-5cm; Described sampling head is cylindric, its diameter be highly preferably 8-9mm.
The embodiment of the present invention additionally provides and utilizes above-mentioned ight soil clonorchis egg collection separation equipment to carry out gathering the method be separated, and comprises the steps:
(A), after first adopting described sampler to take ight soil 0.3-0.35g, described sample bottle is put into inner; (B) sample lid is opened after being tamping by sealing plug, pour liquid into and do not have sample lid on described sampling head bonnet, vertical placement 30-50min is also with sample bottle described in certain interval of time horizontal jitter, and finally standing 3-5min obtains the testing sample containing clonorchis egg; (C) loosen sealing plug after leaving standstill, collect all testing samples and quantitatively detect.
The ight soil clonorchis egg that the present embodiment provides gathers separation method, sampler was used to sample before this, when adopting described sampler to gather ight soil, hand-held sample cap position, sampling head is all immersed in ight soil to make described sampling head be filled up, and preferably mix in the mode of mixing of living while immersing, the sampling head like this with unique texture can take into account stirring and sampling dual-use function.It is finally noted that unnecessary ight soil cotton swab, preferably no more than sampling head volume, is dispelled in order to avoid affect separating effect before detachment process is carried out if exceed needs by sample size.
In the present invention, after sampling, sampler is loaded in tripping device, make sample lid be covered on sample bottle, the sealing plug bottom sample for reference bottle is in closed state, now opens sample lid, pour liquid into, liquid used is the buffer solution of pure water or PH7-7.5, and the volume of liquid is preferably the 75-80% of sample bottle cumulative volume, covers tightly sample lid, vertical placement rolling and adjoint level is shaken, is dispersed to make ight soil.Every 5-10min horizontal jitter sample bottle.Ight soil sediment is not made to block filter screen.Vertically place time standing, high density worm's ovum can be made to fall to completely on filter screen, probably rock 2-3 time altogether at set intervals, be the mesh preventing ight soil sediment from blocking filter screen, be beneficial to worm's ovum and pass through, reach and the worm's ovum of suspension is fully filtered from top layer filter screen as far as possible, reach satisfied first separating effect, wherein after repeatedly vibrating, last time of repose is preferably 2-3min, to prevent the thin ight soil sediment filtered from blocking lower screen, auxiliary worm's ovum filters smoothly.
In the present invention, finally carry out the quantitative detection of separated and collected operation, beat split cavity bottom side sidewall before quantitatively detecting gently and assist worm's ovum to collect gathering, then all testing samples are collected in centrifuge tube, after centrifugation, calculate the number of all clonorchis eggs.As needed qualitative detection, loosen sealing plug gently, liquid in collection unit is fallen; Get the 1st sample liquid and carry out qualitative examination on microslide.Note wanting quiet run when loosening sealing plug, do not make sample liquid fall out of control too soon.About about the 1h of whole operating process, can reach close high density clonorchis egg separating effect completely.
Be further described below by specific embodiment:
The structure front elevation of the ight soil clonorchis egg collection separation equipment that Fig. 1 provides for the first embodiment of the present invention, as can be seen from the figure its concrete structure, be made up of sampler 101 and tripping device 102 two parts, sampler comprises sample lid 103, thief rod 104 and columned sampling head 105, one end of thief rod 104 is fixedly connected with sample lid 103, the other end connects sampling head 105, whole sampler designs for integral type, the upper and lower surface of sampling head 105 is provided with sieve aperture, the diameter of described sieve aperture is 1.6mm, tripping device 102 comprises sample bottle 106, sealing plug 108 and one deck filter screen 107, the bottleneck that sample lid 103 is covered on sample bottle 106 make thief rod 104 and sampling head 105 be positioned at sample bottle 106, sample bottle 106 is divided into two sections, epimere is the sample chamber 110 for holding the sample in sampling head 105, hypomere is the split cavity 111 for separating of the clonorchis egg gone out in sample, split cavity 111 is tapered, sealing plug 108 is arranged at the tapered end of split cavity 111, filter screen 107 is arranged at the intersection of split cavity and sample chamber, and in straight tabular, the mesh order number of filter screen 107 is 100 orders, the bottom of sealing plug 108 is concordant with the bottom of sample bottle 106, the concrete structure of sampler 101 as shown in Figure 5, Fig. 6-8 is the concrete structure of sampling head 105, wherein, Fig. 6 is the structure of sampling head 105 upper surface, Fig. 7 is the structure of sampling head 105 lower surface, be provided with sieve aperture 109 as seen, the concrete shape of sieve aperture 109 then can be seen in fig. 8 clearly.When adopting the ight soil clonorchis egg of embodiment of the present invention collection separation equipment to carry out collection lock out operation, first take ight soil 0.35g with sampler 101, put into sample bottle 106, sample lid 103 is opened after being tamping by sealing plug 108, pour distilled water into and do not have sample lid 103 on sampling head 105 bonnet, vertically place 30min and after certain interval of time horizontal jitter sample bottle 10610s, after standing 3min, obtain the testing sample containing clonorchis egg; Loosen sealing plug 108, collect all testing samples and quantitatively detect.
The structure front elevation of the ight soil clonorchis egg collection separation equipment that Fig. 2 provides for the second embodiment of the present invention, as can be seen from the figure its concrete structure, be made up of sampler 101 and tripping device 102 two parts, sampler comprises sample lid 103, thief rod 104 and columned sampling head 105, one end of thief rod 104 is fixedly connected with sample lid 103, the other end connects sampling head 105, whole sampler designs for integral type, the upper and lower surface of sampling head 105 is provided with sieve aperture, the diameter of described sieve aperture is 1.3mm, between sieve aperture, spacing is 1.2mm, tripping device 102 comprises sample bottle 106, sealing plug 108 and two-layer stainless steel filtering net 107, the bottleneck that sample lid 103 is covered on sample bottle 106 make thief rod 104 and sampling head 105 be positioned at sample bottle 106, sample bottle 106 is divided into two sections, epimere is the sample chamber 110 for holding the sample in sampling head 105, hypomere is the split cavity 111 for separating of the clonorchis egg gone out in sample, split cavity 111 is tapered, sealing plug 108 is arranged at the tapered end of split cavity 111, upper screen 107 is arranged at the intersection of split cavity and sample chamber, lower screen 107 is arranged near sealing plug 108, all in straight tabular, the mesh order number of upper screen 107 is 150 orders, the mesh order number of lower screen 107 is 180 orders, the bottom of sealing plug 108 is concordant with the bottom of sample bottle 106.When adopting the ight soil clonorchis egg of embodiment of the present invention collection separation equipment to carry out collection lock out operation, first take ight soil 0.3g with sampler 101, with coverly during sampling take off sampler 101, sampled voluntarily by patient, hand-held sample lid 103, gos deep into shaping or half-formed ight soil inner by sampling head 105, mix ight soil filled up by sampling head 105 simultaneously by the mode stirred, if sample size exceedes the volume of sampling head 105, then wipe away with cotton swab.Then sample bottle 106 is put into, after sealing plug 108 is tamping, open sample lid 103, pour into for sample bottle cumulative volume 75% PH7-7.5 buffer solution bonnet on sample lid 103, the vertical 50min of placement interval 10min leave standstill the testing sample that 5min obtains containing clonorchis egg gently after horizontal jitter sample bottle 1065s; Loosen sealing plug 108, collect all testing samples in centrifuge tube, after centrifugal or natural sedimentation, under microscope, calculate the number of all clonorchis eggs.
The structure front elevation of the ight soil clonorchis egg collection separation equipment that Fig. 3 provides for the third embodiment of the present invention, as can be seen from the figure its concrete structure, be made up of sampler 101 and tripping device 102 two parts, sampler comprises sample lid 103, thief rod 104 and columned sampling head 105, one end of thief rod 104 is fixedly connected with sample lid 103, the other end connects sampling head 105, whole sampler designs for integral type, the upper and lower surface of sampling head 105 is provided with sieve aperture, the diameter of described sieve aperture is 1.5mm, between sieve aperture, spacing is 1.1mm, tripping device 102 comprises sample bottle 106, sealing plug 108 and three layers of stainless steel filtering net 107, the bottleneck that sample lid 103 is covered on sample bottle 106 make thief rod 104 and sampling head 105 be positioned at sample bottle 106, sample bottle 106 is divided into two sections, epimere is the sample chamber 110 for holding the sample in sampling head 105, hypomere is the split cavity 111 for separating of the clonorchis egg gone out in sample, split cavity 111 is tapered, sealing plug 108 is arranged at the tapered end of split cavity 111, upper screen 107 is arranged at the intersection of split cavity and sample chamber in dome-shaped, middle lower screen 107 is arranged near sealing plug 108, in straight tabular, middle level filter screen 107 is positioned between the two in straight tabular, the mesh order number of upper screen 107 is 100 orders, the mesh order number of middle level filter screen 107 is 150 orders, the mesh order number of lower screen 107 is 200 orders, the bottom of sealing plug 108 is concordant with the bottom of sample bottle 106.When adopting the ight soil clonorchis egg of embodiment of the present invention collection separation equipment to carry out collection lock out operation, first take ight soil 0.3g with sampler 101, with coverly during sampling take off sampler 101, sampled voluntarily by patient, hand-held sample lid 103, gos deep into shaping or half-formed ight soil inner by sampling head 105, mix ight soil filled up by sampling head 105 simultaneously by the mode stirred, if sample size exceedes sampling head 105 volume, then wipe away with cotton swab.Then sample bottle 106 is put into, after sealing plug 108 is tamping, open sample lid 103, pour into for sample bottle cumulative volume 80% distilled water bonnet on sample lid 103, the vertical 40min of placement interval 5min leave standstill the testing sample that 4min obtains containing clonorchis egg gently after horizontal jitter sample bottle 10610s; Last loosening sealing plug 108 gently, gets the 1st sample liquid and carry out qualitative examination on microslide.
The structure front elevation of the ight soil clonorchis egg collection separation equipment that Fig. 4 provides for the present invention's the 4th kind of embodiment, as can be seen from the figure its concrete structure, this embodiment is most preferred embodiment.Be made up of sampler 101 and tripping device 102 two parts, sampler 101 comprises sample lid 103, thief rod 104 and columned sampling head 105, one end of thief rod 104 is fixedly connected with sample lid 103, the other end connects sampling head 105, whole sampler 101 designs for integral type, the upper and lower surface of sampling head 105 is provided with sieve aperture, the diameter of described sieve aperture is 1.5mm, between sieve aperture, spacing is 1mm, tripping device 102 comprises sample bottle 106, sealing plug 108 and two-layer stainless steel filtering net 107, the bottleneck that sample lid 103 is covered on sample bottle 106 make thief rod 104 and sampling head 105 be positioned at sample bottle 106, sample bottle 106 is divided into two sections, epimere is the sample chamber 110 for holding the sample in sampling head 105, hypomere is the split cavity 111 for separating of the clonorchis egg gone out in sample, split cavity 111 is tapered, sealing plug 108 is arranged at the tapered end of split cavity 111, upper screen 107 is arranged at the intersection of split cavity and sample chamber for dome-shaped, lower screen 107 is arranged near sealing plug 108, in straight tabular, the mesh order number of upper screen 107 is 100 orders, the mesh order number of lower screen 107 is 200 orders, the bottom of sealing plug 108 is concordant with the bottom of sample bottle 106.Two-layer filter screen can meet separation requirement, if arrange the filter screen number of plies too much reduce separation efficiency on the contrary, and produces obstruct to the separation of worm's ovum.When adopting the ight soil clonorchis egg of embodiment of the present invention collection separation equipment to carry out collection lock out operation, first take ight soil 0.3g with sampler 101, with coverly during sampling take off sampler 101, sampled voluntarily by patient, hand-held sample lid 103, gos deep into shaping ight soil inner by sampling head 105, mix ight soil filled up by sampling head 105 simultaneously by the mode stirred, if sample size exceedes sampling head 105 volume, then wipe away with cotton swab.Then sample bottle 106 is put into, after sealing plug 108 is tamping, open sample lid 103, pour into for sample bottle cumulative volume 80% distilled water bonnet on sample lid 103, vertical placement 20min interval 5min are gently after horizontal jitter sample bottle 10610s, the middle horizontal jitter sample bottle gently of natural subsidence 30min 1 time, obtains the testing sample containing clonorchis egg after finally leaving standstill 5min; Loosen sealing plug 108, collect all testing samples in 1.5mL plastic centrifuge tube, notice that flow velocity is too not fast, the number calculating all clonorchis eggs after centrifugation quantitatively detects.
Last it is noted that above each embodiment is only in order to illustrate technical scheme of the present invention, be not intended to limit; Although with reference to foregoing embodiments to invention has been detailed description, those of ordinary skill in the art is to be understood that: it still can be modified to the technical scheme described in foregoing embodiments, or carries out equivalent replacement to wherein some or all of technical characteristic; And these amendments or replacement, do not make the essence of appropriate technical solution depart from the scope of various embodiments of the present invention technical scheme.
Claims (10)
1. ight soil clonorchis egg gathers a separation equipment, it is characterized in that, comprising: sampler and tripping device; Described sampler comprises sample lid, thief rod and columned sampling head, one end of described thief rod is fixedly connected with described sample lid, the other end connects described sampling head, and the upper and lower surface of described sampling head is provided with sieve aperture, and the diameter of described sieve aperture is 1.3-1.6mm;
Described tripping device comprises sample bottle, sealing plug and filter screen, and the bottleneck that described sample lid is covered on described sample bottle makes described thief rod and sampling head be positioned at described sample bottle;
Described sample bottle is divided into two sections, epimere is the sample chamber for holding the sample in described sampling head, hypomere is the split cavity for separating of the clonorchis egg gone out in described sample, described split cavity is tapered, described sealing plug is arranged at the tapered end of split cavity, described filter screen is arranged in described split cavity, and the mesh order number of described filter screen is 100-200 order.
2. a kind of ight soil clonorchis egg according to claim 1 gathers separation equipment, and it is characterized in that, the diameter of described sieve aperture is 1.3-1.5mm, and between sieve aperture, spacing is 1-1.2mm.
3. a kind of ight soil clonorchis egg according to claim 1 gathers separation equipment, and it is characterized in that, the material of described filter screen is metal, preferred stainless steel; Described filter screen is layer 2-3, is from top to bottom uniformly distributed in described split cavity.
4. a kind of ight soil clonorchis egg according to claim 3 gathers separation equipment, it is characterized in that, described filter screen is 2 layers, upper screen is dome-shaped, be arranged at described split cavity one end near described sample chamber, mesh order number is 100-150 order, and lower screen arranges near described sealing plug and is straight tabular, and mesh order number is 180-200 order.
5. a kind of ight soil clonorchis egg according to any one of claim 1-4 gathers separation equipment, and it is characterized in that, described sample bottle is cylindric and diameter is 3-4cm, and the height of described sample chamber is 8-9cm, and the height of described split cavity is 4-5cm; Described sampling head is cylindric, its diameter be highly 8-9mm.
6. utilize the ight soil clonorchis egg described in claim 1-5 to gather separation equipment to carry out gathering the method be separated, it is characterized in that, comprise the steps:
(A), after first taking ight soil 0.3-0.35g with described sampler, described sample bottle is put into inner;
(B) sample lid is opened after being tamping by sealing plug, pour liquid into and do not have sample lid on described sampling head bonnet, vertical placement 30-50min is also with sample bottle described in certain interval of time horizontal jitter, and finally standing 3-5min obtains the testing sample containing clonorchis egg;
(C) loosen sealing plug, collect testing sample and quantitatively detect.
7. a kind of ight soil clonorchis egg according to claim 6 gathers separation method, it is characterized in that, in described step (A), when adopting described sampler to gather ight soil, sampling head is all immersed in ight soil to make described sampling head be filled up ight soil.
8. a kind of ight soil clonorchis egg according to claim 6 gathers separation method, it is characterized in that, in described step (B), every 5-10min horizontal jitter sample bottle in vertical put procedure.
9. a kind of ight soil clonorchis egg according to claim 8 gathers separation method, and it is characterized in that, the time of each horizontal jitter sample bottle is 5-10s.
10. a kind of ight soil clonorchis egg according to claim 6 gathers separation method, it is characterized in that, in described step (B), liquid used is the buffer solution of pure water or PH7-7.5, and the volume pouring liquid into is the 75-80% of described sample bottle cumulative volume;
Preferably, in described step (C), the method quantitatively detected comprises: be collected in centrifuge tube by all testing samples, calculate the number of all clonorchis eggs after centrifugation.
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