CN106172263B - Semi-automatic human-excrement schistosoma japonicum egg hatching device and hatching method thereof - Google Patents
Semi-automatic human-excrement schistosoma japonicum egg hatching device and hatching method thereof Download PDFInfo
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- CN106172263B CN106172263B CN201610767124.1A CN201610767124A CN106172263B CN 106172263 B CN106172263 B CN 106172263B CN 201610767124 A CN201610767124 A CN 201610767124A CN 106172263 B CN106172263 B CN 106172263B
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Abstract
A semi-automatic human excrement schistosoma japonicum ovum hatching device and a hatching method thereof belong to the technical field of schistosomiasis control. The device comprises a coarse residue filtering module and an incubation module, wherein the coarse residue filtering module is communicated with the incubation module. The hatching method comprises the steps of firstly placing human excrement in the coarse residue filtering module for water inlet operation, then hatching in the hatching module, and finally collecting effluent liquid of a sample for microscopic observation. The device provided by the invention can be used for rapidly obtaining the detection result, reducing the contact chance between the inspection personnel and the sample, reducing the water consumption and the labor intensity and having the characteristic of stable inspection result.
Description
Technical Field
The invention relates to a semi-automatic human excrement schistosoma japonicum ovum hatching device and a hatching method thereof, belonging to the technical field of schistosomiasis control.
Background
Schistosomiasis japonica is a serious infectious disease which is caused by both human and animal diseases and seriously harms the health of people and influences the development of animal husbandry economy. The gold standard for diagnosing the schistosomiasis mainly comprises the steps of checking schistosome eggs from excrement of a patient or hatching schistosoma miracidium from the excrement. The traditional pipe-jacking method is difficult to operate and pollutes operators, and the degreasing cotton balls (cotton layers) are used for filtering the excrement residues, so that the penetration of cercaria is prevented, the missed detection and the missed diagnosis are caused, and the diagnosis and the prevention of schistosomiasis are influenced.
Therefore, the development of an automatic crushing system, a filtering system and a cercaria permeation system is urgently needed, and the invention of a semi-automatic human-dung schistosoma japonicum ovum hatching device is researched and invented and is used for the etiological diagnosis of human hematuria diseases. The detection rate of the human schistosomiasis can be improved, and the diagnosis accuracy can also be improved.
Disclosure of Invention
The invention aims to provide a semi-automatic human excrement schistosoma japonicum egg hatching device and a hatching method thereof, which can achieve the purpose of schistosoma japonicum etiological diagnosis by stirring, filtering, enriching and hatching human excrement containing schistosoma japonicum eggs.
The invention discloses a semi-automatic human-feces schistosoma japonicum egg hatching device, which comprises a coarse residue filtering module and a hatching module, wherein the coarse residue filtering module is communicated with the hatching module;
the coarse slag filtering module comprises a coarse slag filtering bottle body, an upper cover, a water inlet, a first valve, a stirring paddle and a first screen; the coarse slag filtering bottle body is provided with an upper cover, the upper cover is provided with a water inlet, the first valve is arranged on the water inlet, and the stirring paddle is arranged in the coarse slag filtering bottle body through the upper cover; the first screen is arranged at the bottom of the coarse residue filtering bottle body;
the hatching module comprises a hatching bottle body, a second filter screen, a third filter screen, a waste liquid port, a second valve and a third valve; the top of the hatching bottle body is provided with a third valve, the upper part of the hatching bottle body is provided with a second filter screen, the lower part of the hatching bottle body is provided with a third filter screen, the bottom of the hatching bottle body is provided with a waste liquid port, the second valve is arranged on the waste liquid port, and the third valve is positioned at the upper end of the second filter screen;
the bottom of the coarse residue filtering bottle body is communicated with the hatching bottle body through a pipeline, and one end of the pipeline communicated with the hatching module is positioned between the second filter screen and the third filter screen;
the top of the hatching bottle body is provided with a plurality of scale marks.
The stirring head of the stirring paddle is two cylinders which are crossed in a cross way, and the stirring speed is 100-500 rpm.
The first screen cloth of filter screen is 40 meshes, and the second filter screen is 150 meshes, and the third filter screen is 260 meshes.
The scale of the scale mark is 5, 10, 15 and 20 mL.
The hatching method of the semi-automatic human excrement schistosoma japonicum ovum hatching device comprises the following steps:
(1) water inlet of the coarse slag filtering module: opening the upper cover, firstly filling human excrement containing schistosome eggs into the coarse residue filtering module, covering the upper cover, opening the stirring paddle, opening the first valve on the water inlet to start water inflow to the coarse residue filtering bottle body to form an excrement-water mixture, and enabling the filtrate to enter the incubation module from the connecting pipeline;
(2) water is fed into the incubation module: after entering the incubation module, the filtrate passes through a third filter screen, a second valve is opened, so that the filtrate continuously flows out from the waste liquid port until the liquid manure mixture in the coarse residue filtering module becomes clear, and the second valve, the third valve and the stirring paddle are sequentially closed; the water inlet continues to feed water until the water level in the hatching module reaches a specified scale mark, and the water inlet is closed;
(3) hatching: and (3) illuminating the incubation module, incubating eggs into larvae, enabling the larvae to pass through the second filter screen to enter the upper part of the incubation module, opening a third valve on the sample outflow port after incubation is finished, enabling the sample to flow out, and detecting the flowing-out sample under a microscope.
The invention has the beneficial effects that: the invention relates to a semi-automatic human excrement schistosoma japonicum ovum hatching device which is researched and invented by crushing human excrement by using a crusher and improving a water inlet filtering system and a cercaria permeation system and is used for the etiological diagnosis of schistosomiasis. It not only improves the detection rate of human schistosomiasis, but also improves the accuracy of diagnosis, and is also beneficial to treating schistosomiasis patients and controlling the spread and prevalence of schistosomiasis.
The device provided by the invention can be used for rapidly obtaining the detection result, reducing the contact chance between the inspection personnel and the sample, reducing the water consumption and the labor intensity and having the characteristic of stable inspection result.
Drawings
FIG. 1 is a schematic diagram of the present invention.
Description of reference numerals: 1. a coarse slag filtering module; 1-1, a coarse residue filtering bottle body; 1-2, an upper cover; 1-3, a water inlet; 1-4, a first valve; 1-5, a stirring paddle; 1-6, a first screen; 2. an incubation module; 2-1, incubating the bottle body; 2-2, a second filter screen; 2-3, a third filter screen; 2-4, a waste liquid port; 2-5, a second valve; 2-6 and a third valve.
Detailed Description
Example 1
As shown in figure 1, a semi-automatic human excrement schistosoma japonicum ovum hatching device comprises a coarse residue filtering module 1 and a hatching module 2, wherein the coarse residue filtering module 1 is communicated with the hatching module 2;
the coarse slag filtering module 1 comprises a coarse slag filtering bottle body 1-1, an upper cover 1-2, a water inlet 1-3, a first valve 1-4, a stirring paddle 1-5 and a first screen 1-6; the coarse slag filtering bottle body 1-1 is provided with an upper cover 1-2, the upper cover 1-2 is provided with a water inlet 1-3, a first valve 1-4 is arranged on the water inlet 1-3, and a stirring paddle 1-5 is arranged in the coarse slag filtering bottle body 1-1 through the upper cover 1-2; the first screen 1-6 is arranged at the bottom of the bottle body 1-1;
the hatching module 2 comprises a hatching bottle body 2-1, a second filter screen 2-2, a third filter screen 2-3, a waste liquid port 2-4, a second valve 2-5 and a third valve 2-6; the top of the hatching bottle body 2-1 is provided with a third valve 2-6, the upper part is provided with a second filter screen 2-2, the lower part is provided with a third filter screen 2-3, the bottom is provided with a waste liquid port 2-4, the second valve 2-5 is arranged on the waste liquid port 2-4, and the third valve 2-6 is positioned at the upper end of the second filter screen 2-2;
the bottom of the coarse residue filtering bottle body 1-1 is communicated with the hatching bottle body 2-1 through a pipeline, and one end of the pipeline communicated with the hatching module 2 is positioned between the second filter screen 2-2 and the third filter screen 2-3;
the top of the hatching bottle body 2-1 is provided with a plurality of scale marks.
The stirring heads of the stirring paddles 1-5 are two cylinders crossed in a cross shape, and the stirring speed is 100-500 rpm.
The first screen mesh 1-6 of the filter screen is 40 meshes, the second screen mesh 2-2 is 150 meshes, and the third screen mesh 2-3 is 260 meshes.
The scale of the scale mark is 5, 10, 15 and 20 mL.
Example 2
The hatching method of the semi-automatic human excrement schistosoma japonicum ovum hatching device comprises the following steps:
(1) water inlet of the coarse slag filtering module: opening the upper cover 1-2, firstly filling human excrement containing bloodsucker eggs into the coarse residue filtering module 1, covering the upper cover 1-2, opening the stirring paddle 1-5, opening the first valve 1-4 on the water inlet 1-3 to start water feeding to the coarse residue filtering bottle body 1-1 to form an excrement-water mixture, and enabling the filtrate to enter the hatching module 2 from the connecting pipeline;
(2) water is fed into the incubation module: after entering the hatching module 2, the filtrate passes through a third filter screen 2-3, a second valve 2-5 is opened, so that the filtrate continuously flows out from a waste liquid port 2-4 until the liquid manure mixture in the coarse residue filtering module 1 becomes clear, and the second valve 2-5, a third valve 2-6 and a stirring paddle 1-5 are sequentially closed; the water inlets 1-3 continue to feed water until the water level in the hatching module 2 reaches the designated scale, and the water inlets 1-3 are closed;
(3) hatching: and (3) illuminating the incubation module 2, incubating eggs into larvae, enabling the larvae to pass through the second filter screen 2-2 to enter the upper part of the incubation module 2, opening a third valve 2-6 on the sample outflow port after incubation is finished, enabling the sample to flow out, and detecting the flowing-out sample under a microscope.
Application example 1
The method collects the excrement of schistosomiasis patients in low epidemic areas, and carries out the pathogenic detection of schistosomiasis by using the original excrement detection hatching method and the method of the invention, and the result shows that the invention has high detection positive rate, simple method operation, no pollution and reliable result.
TABLE 1 results of schistosome detection by different methods
Claims (4)
1. A hatching method of a semi-automatic human-feces schistosoma japonicum egg hatching device is characterized in that: the device comprises a coarse residue filtering module (1) and an incubation module (2), wherein the coarse residue filtering module (1) is communicated with the incubation module (2);
the coarse slag filtering module (1) comprises a coarse slag filtering bottle body (1-1), an upper cover (1-2), a water inlet (1-3), a first valve (1-4), a stirring paddle (1-5) and a first screen (1-6); an upper cover (1-2) is arranged on the coarse slag filtering bottle body (1-1), a water inlet (1-3) is arranged on the upper cover (1-2), a first valve (1-4) is arranged on the water inlet (1-3), and a stirring paddle (1-5) is arranged in the coarse slag filtering bottle body (1-1) through the upper cover (1-2); the first screen (1-6) is arranged at the bottom of the coarse residue filtering bottle body (1-1);
the hatching module (2) comprises a hatching bottle body (2-1), a second filter screen (2-2), a third filter screen (2-3), a waste liquid port (2-4), a second valve (2-5) and a third valve (2-6); the top of the hatching bottle body (2-1) is provided with a third valve (2-6), the upper part of the hatching bottle body is provided with a second filter screen (2-2), the lower part of the hatching bottle body is provided with a third filter screen (2-3), the bottom of the hatching bottle body is provided with a waste liquid port (2-4), the second valve (2-5) is arranged on the waste liquid port (2-4), and the third valve (2-6) is positioned at the upper end of the second filter screen (2-2);
the bottom of the coarse residue filtering bottle body (1-1) is communicated with the hatching bottle body (2-1) through a pipeline, and one end of the pipeline communicated with the hatching module (2) is positioned between the second filter screen (2-2) and the third filter screen (2-3);
the top of the hatching bottle body (2-1) is provided with a plurality of scale marks;
the incubation steps are specifically:
(1) water inlet of the coarse slag filtering module: opening the upper cover (1-2), firstly filling human excrement containing hemozoin eggs into the coarse residue filtering module (1), covering the upper cover (1-2), opening the stirring paddle (1-5), opening a first valve (1-4) on the water inlet (1-3) to start to feed water into the coarse residue filtering bottle body (1-1) to form an excrement-water mixture, and enabling the filtrate to enter the hatching module (2) from a connecting pipeline;
(2) water is fed into the incubation module: after entering the hatching module (2), the filtrate passes through a third filter screen (2-3), a second valve (2-5) is opened, so that the filtrate continuously flows out from a waste liquid port (2-4) until the liquid manure mixture in the coarse residue filtering module (1) becomes clear, and the second valve (2-5), the third valve (2-6) and the stirring paddle (1-5) are sequentially closed; the water inlet (1-3) continues to feed water until the water level in the hatching module (2) reaches the designated scale mark, and the water inlet (1-3) is closed;
(3) hatching: and (3) illuminating the incubation module (2), incubating eggs into larvae, enabling the larvae to pass through the second filter screen (2-2) to enter the upper part of the incubation module (2), opening a third valve (2-6) on the sample outflow port after incubation is finished, enabling the sample to flow out, and detecting the flowing-out sample under a microscope.
2. Hatching method according to claim 1, wherein: the stirring heads of the stirring paddles (1-5) are two cylinders crossed in a cross shape, and the stirring speed is 100-500 rpm.
3. Hatching method according to claim 1, wherein: the first screen (1-6) is 40 meshes, the second screen (2-2) is 150 meshes, and the third screen (2-3) is 260 meshes.
4. Hatching method according to claim 1, wherein: the scale of the scale mark is 5, 10, 15 and 20 mL.
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