CN201173892Y - Inversion type egg-collecting direct incubation device - Google Patents

Inversion type egg-collecting direct incubation device Download PDF

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Publication number
CN201173892Y
CN201173892Y CNU2008200569239U CN200820056923U CN201173892Y CN 201173892 Y CN201173892 Y CN 201173892Y CN U2008200569239 U CNU2008200569239 U CN U2008200569239U CN 200820056923 U CN200820056923 U CN 200820056923U CN 201173892 Y CN201173892 Y CN 201173892Y
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CN
China
Prior art keywords
cup
hatching
hasp
lower cover
upper cover
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Expired - Fee Related
Application number
CNU2008200569239U
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Chinese (zh)
Inventor
朱传刚
朱文玉
周扬帆
林矫矫
石耀军
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shanghai Fenyang Middle School
Shanghai Weiyu Middle School
Shanghai Veterinary Research Institute CAAS
Original Assignee
Shanghai Fenyang Middle School
Shanghai Weiyu Middle School
Shanghai Veterinary Research Institute CAAS
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Filing date
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Priority to CNU2008200569239U priority Critical patent/CN201173892Y/en
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Publication of CN201173892Y publication Critical patent/CN201173892Y/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Abstract

The utility model relates to the worm egg collection and direct incubate medical detection appliance technical field, in particular to a detection appliance for schistosomiasis patients and diseased animals. The utility model provides an inversion-type egg collection direct incubator which can directly detect the existence of parasites through simple operation. The device consists of a funnel-shaped lower cover, an incubation cup and a round upper cover. The three separate components are orderly in close fit with each other and then are combined with a closed structure into an integral appliance; the funnel-shaped lower cover is in close matching sealed connection with the incubation cup through a hasp A; the round upper cover is in close matching sealed connection with the incubation cup through a hasp B. The incubator mainly solves relevant technical problems that sieving and incubation are accomplished in one container, and incubation can be promoted through oscillation. The incubator has the advantages that the filtering and incubation of to-be-detected objects can be accomplished in one container, which reduces operation steps and egg loss; the incubator is convenient to shake in incubation process, so as to promote incubation; the incubator can carry out qualitative or quantitative detection according to needs; the incubator raises detection sensitivity, and is simple in structure, convenient to operate, low in cost and suitable for field use.

Description

Inversion type collection ovum is directly incubated device
Technical field
The utility model relates to that a kind of worm egg is collected and the direct medical appliance of hatching, refers to a kind of appliance that is used for blood fluke patient, ill domestic animal especially, and can directly detect the inversion type collection ovum that parasite exists by shirtsleeve operation and directly incubate device.
Background technology
At present, parasitic disease especially Schistosoma japonicum is a kind of serious infecting both domestic animals and human parasitic disease, its etiological diagnosis mainly relies on ight soil miracidium hatching method, ight soil miracidium hatching method is still current the most frequently used detection method as the correct diagnosis of snail fever, the detection method of regulation when also to be that country's examination is up to standard check and accept.Though this each province, method epidemic-stricken area is slightly different, total running program basically identical, this method will be through three road steps: (1) is that the copper sieve filters, and leaches thing and eluriates with the nylon bolting silk; (2) add sediment bowl, precipitation is changed water, removes supernatant; (3) pour in Erlenmeyer flask, the kieldahl flask and hatch, for ease of observing miracidium, also will be under bottleneck 1/2 place add the absorbent cotton of a 2-3cm, add clear water again to bottleneck.This method is commonly referred to conventional nylon bolting silk and eluriates ight soil miracidium hatching method, because this method had both been passed through the copper screen filtration, eluriate through the nylon bolting silk again, the excrement slag is clear, hatching miracidium is easy to observe, but owing to will pass through the copper screen filtration, multisteps such as nylon bolting silk elutriation are rapid, each step some worm's ovums that all will scatter and disappear.So test according to 16 parts of positive ight soil of the Wuxi prevention and cure of schistosomiasis, detect several 1998 of miracidium altogether, be 1171 in the precipitation wherein, only account for 58.6%, and go residual assortedly hatch 827 of miracidiums, account for 38.1% of sum, other change, and water etc. is lost to account for 3.3%.In addition, because operation steps is many, length consuming time.For this reason, be necessary sensitiveer, the method efficiently of development.Another kind of directly stool for hatching method is: (1) repeated precipitation in sediment bowl is changed water; (2) pour in Erlenmeyer flask, the kieldahl flask absorbent cotton that 1/2 place under (3) bottleneck adds a 2-3cm into, add clear water again to bottleneck.Because ight soil filters without copper sieving cup, not eluriate again through the nylon bolting silk, the excrement slag is many, and pigment is dense, influences miracidium and hatches and follow-up observation.Shen Jie 50 test findings in Shanghai show that hatching the miracidium number under the available light is 7355, and only hatches 1990 of miracidiums under the dark situations, differs to reach 3 times more than.Summary is got up, and the shortcoming of the method for these two kinds of hatchings all is: (1) is low to the susceptibility of low infectiosity crowd or animal; (2) worm's ovum is lost manyly, counts inaccurate; (3) step is many, length consuming time.
Summary of the invention
In order to overcome above-mentioned weak point, fundamental purpose of the present utility model aims to provide a kind of device of finishing the filtration and the hatching of determinand in a container, realizes a kind of susceptibility height, count enable, expense is low, and is easy to operate, is applicable to that the inversion type collection ovum of on-the-spot use is directly incubated device.
The technical problems to be solved in the utility model is: solve this hatching utensil and how reduce step through the copper screen filtration, how help miracidium observation problem; Solve this hatching utensil and how to reduce the conversion of determinand in different vessels, reduce operation steps problems such as adding tampon; Solving to filter influences the residual assorted of hatching effect and observation effect, and can easily realize sieving and finish in a container and can promote relevant technologies problem such as hatching by concussion with hatching.
The technical scheme that its technical matters that solves the utility model adopts is: this device is made up of infundibulate lower cover, hatching cup and circular upper cover, after three parts of this separation closely cooperate mutually successively, be combined as holistic utensil in conjunction with enclosed construction, the infundibulate lower cover closely mates sealing by hasp A with the hatching cup and is connected, circular upper cover closely mates sealing by hasp B with the hatching cup and is connected, wherein:
The top center position of one infundibulate lower cover is provided with the opening observation tube, the bottom is provided with and hatches the hasp A that cup is combined closely, observation tube and hasp A respectively with the infundibulate lower cover body structure that permeates, the inside of infundibulate lower cover is provided with and the groove A of hatching glass end opening tight connecting, be provided with size shape and the consistent elastic cushion of hatching cup rim of a cup in the groove A, is connected for tight coupling sealing between elastic cushion and the rim of a cup, the bottom of sealing hatching glass is combined as the cup body structure of lower seal;
One circular upper cover is provided with and hatches the hasp B that cup is combined closely, hasp B and the circular upper cover body structure that permeates, the inside of circular upper cover is provided with and the groove B of hatching cup tight connecting suitable for reading, be provided with size shape and the consistent elastic cushion of hatching cup in the groove B, the top of cup is hatched in sealing, is combined as the cup body structure of top seal;
What the top of one hatching cup was provided with convex goes up a cup edge, and the bottom is provided with the following glass edge of convex, last cup edge and following glass of edge respectively with the cup of the hatching glass body structure that permeates; Mutually by being pressed into fastening after the wringing fit, hatch the bottom of cup in conjunction with sealing between the hasp A of following cup edge and infundibulate lower cover; Mutually by being pressed into fastening after the wringing fit, hatch a cup top between the hasp B of last cup edge and circular upper cover in conjunction with sealing; The inner card of hatching cup has the fine sand net, is by the colloid fixed connection between fine sand net and the hatching cup.
Hasp A and hasp B that described inversion type collection ovum is directly incubated device respectively are distributed as 4 hasps along the even position of circumference respectively.
The pore size that described inversion type collection ovum is directly incubated the fine sand net of device is 5-100 μ m.
The material that described inversion type collection ovum is directly incubated infundibulate lower cover main body, hatching cup and the circular upper cover of device is transparent plastics.
Described inversion type collection ovum directly incubate device the infundibulate lower cover the opening observation tube material or for transparent glass or be plastics.
The beneficial effects of the utility model are: this hatching utensil has reduced the step through the copper screen filtration, directly with the inhibition egg hatch material in the nylon bolting silk elutriation ight soil minimizing excrement slag, helps miracidium observation again.Simultaneously, reduce the conversion of determinand in different vessels, reduce operation stepss such as adding tampon, can not only filter influence the residual assorted of hatching effect and observation effect, and can easily realize sieving and finish in a container and can promote to hatch by concussion with hatching; Be implemented in filtration and the hatching of finishing determinand in the container, reduced the loss of operation steps and worm's ovum; In hatching process, conveniently rock, promote hatching; Can carry out qualitative or detection by quantitative as required; Improve the susceptibility that detects, simple in structure, easy to operate, with low cost, be suitable for on-the-spot the use.
Description of drawings
Below in conjunction with drawings and Examples the utility model is further specified.
Accompanying drawing 1 is the longitudinal profile organigram that the utility model splits;
Accompanying drawing 2 is outward appearance and the profile construction synoptic diagram after the combination among the utility model embodiment.
Label declaration in the accompanying drawing:
The 1-observation tube; The 8-circular upper cover;
2-infundibulate lower cover; 9-groove A;
3-hasp A; The 10-elastic cushion;
4-fine sand net; The 11-rim of a cup;
The following glass edge of 5-; 13-hasp B;
6-is hatched cup; 19-groove B;
The last cup of 7-edge;
Embodiment
See also shown in the accompanying drawing 1,2, the utility model is made up of parts such as infundibulate lower cover 2, hatching cup 6 and circular upper covers 8, after three parts of this separation closely cooperate mutually successively, be combined as holistic utensil in conjunction with enclosed construction, infundibulate lower cover 2 closely mates sealing by hasp A 3 with hatching cup 6 and is connected, circular upper cover 8 closely mates sealing by hasp B 13 with hatching cup 6 and is connected, wherein:
The top center position of one infundibulate lower cover 2 is provided with opening observation tube 1, the bottom is provided with and hatches the cup 6 hasp A 3 that combine closely, observation tube 1 and hasp A 3 respectively with infundibulate lower cover 2 body structure that permeates, the inside of infundibulate lower cover 2 is provided with and the groove A 9 of hatching cup 6 end opening tight connectings, be provided with the consistent elastic cushion 10 of size shape and hatching cup 6 rims of a cup 11 in the groove A 9, be connected for tight coupling sealing between elastic cushion 10 and the rim of a cup 11, the bottom of cup 6 is hatched in sealing, is combined as the cup body structure of lower seal;
One circular upper cover 8 is provided with and hatches the cup 6 hasp B 13 that combine closely, hasp B 13 and circular upper cover 8 body structure that permeates, the inside of circular upper cover 8 is provided with and the groove B19 of hatching cup 6 tight connectings suitable for reading, be provided with the consistent elastic cushion 10 of size shape and hatching cup 6 in the groove B 19, the top of cup 6 is hatched in sealing, is combined as the cup body structure of top seal;
What the top of one hatching cup 6 was provided with convex goes up cup along 7, and the bottom is provided with following glass of convex along 5, last cup along 7 and following glass along 5 respectively with the cup of hatching glasss 6 body structure that permeates; Following cup along 5 with the hasp A 3 of infundibulate lower cover 2 between mutually by being pressed into fastening after the wringing fit, hatch glasss 6 bottom in conjunction with sealing; Last cup along 7 with the hasp B 13 of circular upper cover 8 between mutually by being pressed into fastening after the wringing fit, hatch a glass top in conjunction with sealing; Hatching cup 6 inner cards have fine sand net 4, are by the colloid fixed connection between fine sand net 4 and the hatching cup 6.
Hasp A 3 and hasp B 13 that described inversion type collection ovum is directly incubated device respectively are distributed as 4 hasps along the even position of circumference respectively.
The pore size that described inversion type collection ovum is directly incubated the fine sand net 4 of device is 5-100 μ m.
The material that described inversion type collection ovum is directly incubated infundibulate lower cover 2 main bodys, hatching cup 6 and the circular upper cover 8 of device is transparent plastics.
Described inversion type collection ovum directly incubate device infundibulate lower cover 2 opening observation tube 1 material or for transparent glass or be plastics.
Technical characterictic of the present utility model and principle of work are as follows:
Post the fine sand net of 5-100 μ m in the hatching cup 6 of the present utility model, testing sample washes in the fine sand net, is filtered less than the impurity and the pigment of fine sand screen distance and removes, and worm's ovum to be measured obtains reservation greater than the fine sand screen distance.Add and to seal hatching cup two, prevent the upper and lower cover that seeps water.Loam cake is a flattened round, can conveniently hatch cup and keep flat, and lower cover is an infundibulate, top end opening, the come-up that conveniently adds water and hatch miracidium.Behind the sealing hatching cup upper and lower part, be inverted hatching cup 6, add the water hatching from the opening part of infundibulate lower cover 2 observation tubes, the miracidium that egg hatch becomes has phototropism and property upwards, converges to view window.Whether when only needing to do qualitative detection, can observe by observation tube 1 has miracidium to occur in the water body; When needs are done detection by quantitative, can collect whole miracidiums by the moisture that filters in the cup, reach quantitative effect.
In Fig. 1, the top of infundibulate lower cover 2 has can be for the observation tube 1 of observation, the bottom of infundibulate lower cover 2 have can with hatching cup 6 hasp A 3 that combine, flexible pads 10 in the groove A 9 of infundibulate lower cover 2 inside, can seal the bottom of hatching cup 6, prevent the bottom infiltration.The fine sand net 4 of 5-100 μ m is posted in hatching cup 6 inside, play cup along 5 and last cup can be respectively combine sealing along 7 and hatch glass 6 top and the bottom with the hasp A 3 of the hasp B 13 of circular upper cover 8 and infundibulate lower cover 2.Circular upper cover 8 have can with hatching cup 6 hasp B 13 that combine, flexible pads 10 in the groove B 19 of circular upper cover 8 inside can seal the top of hatching cup 6, prevent the top infiltration.
Collect, hatch embodiment of explanation shown in 2 in conjunction with the accompanying drawings with japonice ovum below, testing sample places the fine sand net 4 of hatching cup 6, is washed with flowing water, till current are limpid.Cover circular upper cover 8, with the hasp B 13 of circular upper cover 8 with hatching cup 6 go up cup along 7 combinations, the consistent cushion 10 of size shape and hatching rim of a cup 11 is arranged, the cup of formation top seal in the loam cake internal recess B 19.After the inversion, cover infundibulate lower cover 2, make up along 5 with following glass of hatching cup 6 with the hasp A 3 of infundibulate lower cover 2, size shape and the consistent cushion 10 of hatching rim of a cup 11 are arranged in infundibulate lower cover 2 internal recess 9, form the cup of the same sealing in bottom, inject the hatching water from the observation tube 1 of infundibulate lower cover 2, under appropriate condition, realize the hatching of worm's ovum.
Detect different worm's ovums and can select different apertures fine sand net for use.

Claims (5)

1, a kind of inversion type collection ovum is directly incubated device, it is characterized in that: this device is made up of infundibulate lower cover (2), hatching cup (6) and circular upper cover (8), after three parts of this separation closely cooperate mutually successively, be combined as holistic utensil in conjunction with enclosed construction, infundibulate lower cover (2) closely mates sealing by hasp A (3) with hatching cup (6) and is connected, circular upper cover (8) closely mates sealing by hasp B (13) with hatching cup (6) and is connected, wherein:
The top center position of one infundibulate lower cover (2) is provided with opening observation tube (1), the bottom is provided with and hatches the hasp A (3) that cup (6) is combined closely, observation tube (1) and hasp A (3) respectively with infundibulate lower cover (2) body structure that permeates, the inside of infundibulate lower cover (2) is provided with and the groove A (9) of hatching cup (6) end opening tight connecting, be provided with size shape and the consistent elastic cushion (10) of hatching cup (6) rim of a cup (11) in the groove A (9), be connected for tight coupling sealing between elastic cushion (10) and the rim of a cup (11), the bottom of cup (6) is hatched in sealing, is combined as the cup body structure of lower seal;
One circular upper cover (8) is provided with and hatches the hasp B (13) that cup (6) is combined closely, hasp B (13) and circular upper cover (8) body structure that permeates, the inside of circular upper cover (8) is provided with and the groove B (19) of hatching cup (6) tight connecting suitable for reading, be provided with size shape and hatching cup (6) consistent elastic cushion (10) in the groove B (19), the top of cup (6) is hatched in sealing, is combined as the cup body structure of top seal;
What the top of one hatching cup (6) was provided with convex goes up cup along (7), and the bottom is provided with following glass of convex along (5), last cup along (7) and following glass of edge (5) respectively with the cup of hatching glass (6) body structure that permeates; Mutually by being pressed into fastening after the wringing fit, hatch the bottom of cup (6) in conjunction with sealing between the hasp A (3) of following cup edge (5) and infundibulate lower cover (2); Mutually by being pressed into fastening after the wringing fit, hatch a cup top between the hasp B (13) of last cup edge (7) and circular upper cover (8) in conjunction with sealing; Hatching cup (6) inner card has fine sand net (4), is by the colloid fixed connection between fine sand net (4) and the hatching cup (6).
2, inversion type collection ovum according to claim 1 is directly incubated device, it is characterized in that: described hasp A (3) and hasp B (13) respectively are distributed as 4 hasps along the even position of circumference respectively.
3, inversion type collection ovum according to claim 1 is directly incubated device, it is characterized in that: the pore size of described fine sand net (4) is 5-100 μ m.
4, inversion type collection ovum according to claim 1 is directly incubated device, it is characterized in that: the material of described infundibulate lower cover (2) main body, hatching cup (6) and circular upper cover (8) is transparent plastics.
5, inversion type collection ovum according to claim 1 is directly incubated device, it is characterized in that: the material of the opening observation tube (1) of described infundibulate lower cover (2) or for transparent glass or be plastics.
CNU2008200569239U 2008-04-03 2008-04-03 Inversion type egg-collecting direct incubation device Expired - Fee Related CN201173892Y (en)

Priority Applications (1)

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Application Number Priority Date Filing Date Title
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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105092829A (en) * 2015-07-28 2015-11-25 李召军 Detection device for schistosome disease
CN106172262A (en) * 2016-08-31 2016-12-07 江苏省血吸虫病防治研究所 A kind of semi-automatic beast excrement schistosoma japonice ovum hatching apparatus and hatching method thereof
CN106172263A (en) * 2016-08-31 2016-12-07 江苏省血吸虫病防治研究所 A kind of semi-automatic human excrement schistosoma japonice ovum hatching apparatus and hatching method thereof
CN107410219A (en) * 2017-05-22 2017-12-01 谢曙英 A kind of japonice ovum separator

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105092829A (en) * 2015-07-28 2015-11-25 李召军 Detection device for schistosome disease
CN106172262A (en) * 2016-08-31 2016-12-07 江苏省血吸虫病防治研究所 A kind of semi-automatic beast excrement schistosoma japonice ovum hatching apparatus and hatching method thereof
CN106172263A (en) * 2016-08-31 2016-12-07 江苏省血吸虫病防治研究所 A kind of semi-automatic human excrement schistosoma japonice ovum hatching apparatus and hatching method thereof
CN106172263B (en) * 2016-08-31 2021-12-21 江苏省血吸虫病防治研究所 Semi-automatic human-excrement schistosoma japonicum egg hatching device and hatching method thereof
CN107410219A (en) * 2017-05-22 2017-12-01 谢曙英 A kind of japonice ovum separator

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GR01 Patent grant
C17 Cessation of patent right
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20081231

Termination date: 20100403