CN106124713A - Metacercaria of liver flukes separation equipment and using method thereof - Google Patents
Metacercaria of liver flukes separation equipment and using method thereof Download PDFInfo
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- CN106124713A CN106124713A CN201610419911.7A CN201610419911A CN106124713A CN 106124713 A CN106124713 A CN 106124713A CN 201610419911 A CN201610419911 A CN 201610419911A CN 106124713 A CN106124713 A CN 106124713A
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- separation
- drainage screen
- room
- metacercaria
- separation equipment
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/02—Food
- G01N33/12—Meat; fish
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
Abstract
The invention provides a kind of metacercaria of liver flukes separation equipment and using method thereof, separation equipment includes: first separation chamber, upper cover, precipitation separation room, example enrichment room, sample divider and support；Just it is provided with the first drainage screen at the upper shed of separation chamber；Upper cover is dismountable with first separation chamber to be connected；Precipitation separation room is connected with first separation chamber and connects, and connectivity part is provided with the second drainage screen；Example enrichment room is connected with precipitation separation room and connects, and is provided with the 3rd drainage screen in connectivity part；Sample divider is dismountable with example enrichment room to be connected and connects；Support is connected with the lower end of first separation chamber, and can be supported in the horizontal plane by separation equipment.The separation equipment that the present invention provides, make tissue digest through the filtration precipitation concentration of three times, instead of gradation of the prior art washing, the loss of bladder worm when avoiding repeatedly washing, simultaneously, bladder worm is fully enriched in sample divider, carries out disposable microscopy so that it is easily carry out according to standardization.
Description
Technical field
The present invention relates to food safety detection and prevention and control of diseases technical field, especially relate to a kind of metacercaria of liver flukes
Separation equipment and the using method of this separation equipment.
Background technology
At present, from the biological tissues such as the flesh of fish, separate metacercaria of liver flukes and use water washing and precipitating method after digestion.Detailed process is:
Put into after taking the chopping of the biological tissue such as 30 to 50 grams of flesh of fish or blending 5 to 10 times of volumes Digestive system (containing 1% pepsin and
1% hydrochloric acid), digest 16 hours under 37 DEG C of environment, then digest is crossed screen cloth and removes the slaking residue of bulk, comprise bladder worm
Filtrate load in conical vessel 100' as shown in Figure 1, fill it up with clear water or buffer solution for cleaning；Through natural sedimentation at least 20 minutes
After outwell supernatant liquid, retain lower floor's precipitate；Continue to fill it up with clear water or buffer solution for cleaning, so repeat 4 to 5 times to major part
Impurity is removed.Outwell overwhelming majority supernatant liquid, leave and take and comprise bladder worm precipitate a little under low power microscope or anatomical lens
Observe.
Existing separation metacercaria of liver flukes is without special equipment, and its process uses simple glass bottle, metallic screen, conical graduate etc. to replace
For thing, wherein, relation bladder worm separates the most successfully water washing and precipitating process to be needed to be iteratively repeated repeatedly to carry out, and its shortcoming is main
For:
(1) water-washing process anthropic factor impact is big, is iteratively repeated this process and is easily lost bladder worm；
(2) the many impurity of the concentrate containing bladder worm and volume after water washing and precipitating are excessive, are unfavorable for microscopy；
(3) whole operating process is random strong, it is not easy to standardization.Testing result can be caused bigger mistake by disadvantages mentioned above
Difference；
(4) last, repeatedly water-washing process is the most more, and whole water washing and precipitating processes at least need 90 minutes.
Summary of the invention
In order to solve at least one technical problem of above-mentioned existence, it is an object of the present invention to provide a kind of structure letter
The loss of bladder worm when single, minimizing is repeatedly washed, and can disposably complete the metacercaria of liver flukes separation equipment of microscopy.
Further object is that offer a kind of using method for above-mentioned separation equipment, it is possible to make operation by
Carry out according to standardization, reduce manpower and materials, and the operating time is short.
The metacercaria of liver flukes separation equipment that first aspect present invention provides, including:
Just separation chamber, the described just two ends of separation chamber offer respectively and set at upper shed and under shed, and described upper shed
It is equipped with the first drainage screen；
Upper cover, described upper cover is with described just separation chamber is dismountable is connected, and can seal described upper shed；
Precipitation separation room, the upper end of described precipitation separation room is connected with the lower end of described just separation chamber, and with described under
Opening in communication, and described lower opening is provided with the second drainage screen；
Example enrichment room, the upper end of described example enrichment room is connected with the lower end of described precipitation separation room and connects, and
Described example enrichment room is provided with the 3rd drainage screen with the connectivity part of described precipitation separation room；
Sample divider, the upper end of described sample divider has an opening, and described opening part and described example enrichment room
The dismountable connection in lower end and connect；With
Support, described support is connected with the lower end of described just separation chamber, and can be supported by metacercaria of liver flukes separation equipment
In the horizontal plane, and between the supporting surface at described sample divider and described support place, there is gap.
In technique scheme, it is preferred that described just separation chamber is tubular, and described precipitation separation room and described sample are rich
Collection room is the most tapered.
Further, described just separation chamber is cylindrical, and described precipitation separation room and described example enrichment room are respectively in circle
Taper.
Such being designed to makes the clean separation thing rapid precipitation after the second drainage screen concentrate, and make after separation is pure
Clean bladder worm, in example enrichment room rapid precipitation to sample divider, improves the concentrating and precipitating time of product, and then improves
The work efficiency of product.
In any of the above-described technical scheme, it is preferred that described first drainage screen is recessed net, and described first drainage screen is resistance to
The metal material of acid is made, and the periphery of described first drainage screen is provided with metal edge frame.
Further, the mesh count of described first drainage screen is 20 to 30 mesh.
In any of the above-described technical scheme, it is preferred that described second drainage screen and described 3rd drainage screen are respectively plain net,
And described second drainage screen and described 3rd drainage screen is respectively acidproof metal material or nylon is made, and in described second mistake
The periphery of filter screen and described 3rd drainage screen is respectively arranged with metal edge frame.
Further, the mesh count of described second drainage screen is 40 to 50 mesh, the mesh count of described 3rd drainage screen
It is 50 to 70 mesh.
In any of the above-described technical scheme, it is preferred that described metacercaria of liver flukes separation equipment also includes sample collection portion；
The mating shapes of the shape of described sample collection portion and described sample divider, and described sample collection portion is upper
End is connected with the lower end of described example enrichment room and connects, and the lower end of described sample collection portion is opened with described sample divider
Dismountable connection connecting at Kou.
Further, the height of described support is received more than described precipitation separation room, described example enrichment room and described sample
The height sum in collection portion, during so that described collection containers is connected with described sample collection portion, described sample divider and institute
State, between the supporting surface at support place, there is gap.
In any of the above-described technical scheme, it is preferred that the opening part of described sample divider is provided with female thread, described sample
The lower end in product collection portion is provided with the external screw thread matched with described female thread.
Further, the female thread of the opening part of described sample divider account for described sample divider height three/
One.
In any of the above-described technical scheme, it is preferred that be provided with external screw thread at the upper shed of described just separation chamber, described on
Cover and be provided with the female thread matched with described external screw thread；Or, it is provided with Access Division at the upper shed of described just separation chamber, institute
State to cover and be provided with the buckle matched with described Access Division.
In any of the above-described technical scheme, it is preferred that described just separation chamber, described precipitation separation room and described example enrichment
It is respectively arranged with scale value in the outside wall surface of room.
The present invention provide metacercaria of liver flukes separation equipment, through first separation chamber, precipitation separation room, example enrichment room, sample
The optimization of product catcher and support is integrated, then through the first drainage screen, the second drainage screen and the setting of the 3rd drainage screen, makes digestion
After tissue digest through the filtration precipitation concentration of three times, instead of gradation of the prior art washing, it is to avoid repeatedly wash
The loss of bladder worm when washing, simultaneously, moreover it is possible to effectively remove the bulk impurity after tissue digestion, can be 150 microns by size
Bladder worm be fully enriched in sample divider, and then carry out disposable microscopy so that it is easily carry out according to standardization, reduce people
Power material resources, reduce cost, improve the service efficiency of product.
The using method of the metacercaria of liver flukes separation equipment that second aspect present invention provides, including:
Step S1, opens upper cover, adds about 300mL normal saline or clean water, back-outs sample divider, makes liquid complete
It is full of bottom separation equipment, does not stay bubble, tighten sample divider；
Step S2, supplementary normal saline or clean water are to 300mL scale value position, by postdigestive tissue digest about
300mL pours in the first drainage screen, makes liquid naturally fall, and after addition, liquid level is located approximately at 600mL scale value position；
Step S3, covers tightly upper cover, and vertical standing 30 minutes to 40 minutes, at the beginning of tapping gently according to horizontal direction during this period
Separation chamber's part 2 to 3 times, makes to pass under vibrations, and auxiliary bladder worm passes through the second drainage screen and the 3rd drainage screen；
Step S4, keeps upper cover for covering tightly state, turns round lower sample divider, and the bladder worm centralized collection after precipitation is received at sample
The latter half of storage；
Step S5, draws and is totally separated thing to microscope slide or little plate, low power microscope or dissection Microscopic observation bladder worm；
Step S6, opens upper cover, outwells liquid, fully clean separation equipment after use, carry out after drying
Next time uses.
Further, after step S2, also include step S201, it is simply that after step S2 before step S3, mention ground floor
Drainage screen, discards postdigestive tissue digest residue.
Use this separation equipment that metacercaria of liver flukes in postdigestive tissue digest is carried out separation and Extraction, it is thus achieved that separation
Thing high purity, it is possible to disposably complete microscopy, meanwhile, carry out operation with this separation equipment and decrease manpower and materials, reduce
The running cost of product, it addition, eliminate the process of multistep washing, makes the operating time be greatly reduced, improves product separation
Efficiency, and then improve the serviceability of product.
Additional aspect and the advantage of the present invention will become obvious in following description part, or by the practice of the present invention
Recognize.
Accompanying drawing explanation
In order to be illustrated more clearly that the specific embodiment of the invention or technical scheme of the prior art, below will be to specifically
In embodiment or description of the prior art, the required accompanying drawing used is briefly described, it should be apparent that, in describing below
Accompanying drawing is some embodiments of the present invention, for those of ordinary skill in the art, before not paying creative work
Put, it is also possible to obtain other accompanying drawing according to these accompanying drawings.
Fig. 1 is the perspective view of conical vessel of the prior art；
The structural representation of the metacercaria of liver flukes separation equipment that Fig. 2 provides for the embodiment of the present invention；
Fig. 3 is the top view of the first drainage screen shown in Fig. 1；
Fig. 4 is the structural representation of the sample divider section view shown in Fig. 1；
The using method flow chart of the metacercaria of liver flukes separation equipment that Fig. 5 provides for another embodiment of the present invention；Accompanying drawing mark
Note:
1-upper cover；2-just separation chamber；3-precipitation separation room；
4-example enrichment room；5-sample divider；6-sample collection portion；
7-the first drainage screen；8-the second drainage screen；9-the 3rd drainage screen；
10-support；51-female thread；71-metal edge frame；
100'-conical vessel.
Detailed description of the invention
Below in conjunction with accompanying drawing, technical scheme is clearly and completely described, it is clear that described enforcement
Example is a part of embodiment of the present invention rather than whole embodiments.Based on the embodiment in the present invention, ordinary skill
The every other embodiment that personnel are obtained under not making creative work premise, broadly falls into the scope of protection of the invention.
In describing the invention, it should be noted that term " " center ", " on ", D score, "left", "right", " vertically ",
Orientation or the position relationship of the instruction such as " level ", " interior ", " outward " they are based on orientation shown in the drawings or position relationship, merely to
Be easy to describe the present invention and simplifying describe rather than instruction or the hint device of indication or element must have specific orientation,
With specific azimuth configuration and operation, therefore it is not considered as limiting the invention.Additionally, term " first ", " second ",
" the 3rd " is only used for describing purpose, and it is not intended that indicate or hint relative importance.
In describing the invention, it should be noted that unless otherwise clearly defined and limited, term " is installed ", " phase
Even ", " connection " should be interpreted broadly, for example, it may be fixing connection, it is also possible to be to removably connect, or be integrally connected；Can
To be mechanical connection, it is also possible to be electrical connection；Can be to be joined directly together, it is also possible to be indirectly connected to by intermediary, Ke Yishi
The connection of two element internals.For the ordinary skill in the art, can understand that above-mentioned term is at this with concrete condition
Concrete meaning in invention.
The structural representation of the metacercaria of liver flukes separation equipment that Fig. 2 provides for the embodiment of the present invention；Fig. 3 is shown in Fig. 1
The top view of the first drainage screen；Fig. 4 is the structural representation of the sample divider section view shown in Fig. 1；As in Figure 2-4, this
The metacercaria of liver flukes separation equipment that the embodiment of bright first aspect provides, including: first separation chamber 2, upper cover 1, precipitation separation room 3,
Example enrichment room 4, sample divider 5 and support 10；
Specifically, the two ends of described just separation chamber 2 offer respectively and arrange at upper shed and under shed, and described upper shed
There is the first drainage screen 7；Described upper cover 1 is with described just separation chamber 2 is dismountable is connected, and can seal described upper shed；Described
The upper end of precipitation separation room 3 is connected with the lower end of described just separation chamber 2, and is connected with described under shed, and described under open
The second drainage screen 8 it is provided with at Kou；The upper end of described example enrichment room 4 is connected with the lower end of described precipitation separation room 3 and connects
Lead to, and the connectivity part of described example enrichment room 4 and described precipitation separation room 3 is provided with the 3rd drainage screen 9；Described sample divider
The upper end of 5 has an opening, and described opening part is dismountable with the lower end of described example enrichment room 4 is connected and connects；Described
Frame 10 is connected with the lower end of described just separation chamber 2, and can be supported in the horizontal plane by metacercaria of liver flukes separation equipment, and described
Between the supporting surface at sample divider 5 and described support place, there is gap.
As in figure 2 it is shown, in one embodiment of the invention, further, described just separation chamber 2 in tubular, described point
The most tapered from mud chamber 3 and described example enrichment room 4.
Further, described just separation chamber 2 is cylindrical, described precipitation separation room 3 and described example enrichment room 4 respectively in
Cone.
In this embodiment, such being designed to makes the clean separation thing rapid precipitation after the second drainage screen 8 dense
Contracting, makes the pure bladder worm after separation in example enrichment room 4 rapid precipitation to sample divider 5, improves the concentration of product
Sedimentation time, and then improve the work efficiency of product.
As shown in Figures 2 and 3, in one embodiment of the invention, further, described first drainage screen 7 is recessed net,
And the metal material that described first drainage screen 7 is acidproof makes, and the periphery of described first drainage screen 7 is provided with metal edge frame
71。
Further, acid resistant metal material is copper or aluminum, certainly, can also select for a person skilled in the art
Other acid metal, all should be within the protection domain of the application.
In this embodiment, the first drainage screen 7 is recessed net, and makes for acidproof metal material, on the one hand, add
The intensity of one drainage screen 7, improves the reliability of product, and on the other hand, the design of recessed net adds the filtration of the first drainage screen 7
Area, it is ensured that smoothly completing of first separation, improves the serviceability of product.
As in figure 2 it is shown, in one embodiment of the invention, further, described second drainage screen 8 and described 3rd mistake
Filter screen 9 is respectively plain net, and described second drainage screen 8 and described 3rd drainage screen 9 are respectively acidproof metal material or nylon
Make, and be respectively arranged with metal edge frame at the periphery of described second drainage screen 8 and described 3rd drainage screen 9.
Further, the diameter of described second drainage screen 8 is identical with the diameter of described first drainage screen 7.
In this embodiment, the second drainage screen 8 and the 3rd drainage screen 9 are respectively plain net, add the filtration effect of digest
Rate, improves product separation efficiency, and meanwhile, the second drainage screen 8 and the 3rd drainage screen 9 are respectively metal material or nylon system
Become, and be both provided with metal edge frame at the periphery of drainage screen, this adds increased the second drainage screen 8 and structure of the 3rd drainage screen 9
Intensity, improves the reliability of product.
As in figure 2 it is shown, in one embodiment of the invention, further, described metacercaria of liver flukes separation equipment is also
Including sample collection portion 6；
The shape of described sample collection portion 6 and the mating shapes of described sample divider 5, and described sample collection portion 6
Upper end be connected with the lower end of described example enrichment room 4 and connect, the lower end of described sample collection portion 6 and described sample collection
The dismountable connection of opening part of device 5 also connects.
In this embodiment, the setting of sample collection portion 6, and with the mating shapes of sample divider 5, make sample collection
Device 5 is convenient to the collection of separator bladder worm, meanwhile, also makes the installation of sample divider 5 be more prone to dismounting, further
The operating efficiency that improve product.
Further, the height of described support 10 is more than described precipitation separation room 3, described example enrichment room 4 and described sample
The height sum in product collection portion 6, during so that described collection containers is connected with described sample collection portion 6, described sample collection
Between the supporting surface at device 5 and described support 10 place, there is gap.
The height of support 10, more than the height summation of above-mentioned three, facilitates taking of sample divider 5.
As shown in Figure 2 and Figure 4, in one embodiment of the invention, further, the opening of described sample divider 5
Place is provided with female thread 51, and the lower end of described sample collection portion 6 is provided with the external screw thread matched with described female thread 51.
Further, the female thread of the opening part of described sample divider 5 account for described sample divider 5 height three/
One.
In this embodiment, the opening part of sample divider 5 is provided with female thread, and the lower end of sample collection portion 6 is provided with
The external screw thread matched with female thread, is spirally connected convenient and simple, improves the operating efficiency of product, meanwhile, spiral shell in sample divider 5
At the setting of stricture of vagina, and the top of sample divider 5 and 1/3rd, it is possible to make liquid quickly fill, female thread makes product be difficult to
Retain bubble, facilitate the fast operating of product.
In one embodiment of the invention, further, at the upper shed of described just separation chamber 2, it is provided with external screw thread,
It is provided with, on described upper cover 1, the female thread matched with described external screw thread；Or, it is provided with at the upper shed of described just separation chamber 2
Access Division, described upper cover 1 is provided with the buckle matched with described Access Division.
In this embodiment, first separation chamber 2 arranges the female thread bolt of external screw thread and upper cover 1, facilitates the lid of upper cover 1
If, and beneficially upper cover 1 covers tightly the upper shed of just separation chamber 2, improves efficiency of assembling and the reliability of product；Additionally, it is possible to
To be provided with Access Division in first separation chamber 2, upper cover 1 arranges buckle, make upper cover 1 and first separation chamber 2 carry out clamping, convenient product
Product assemble, and improve the operating efficiency of product.
In one embodiment of the invention, further, described just separation chamber 2, described precipitation separation room 3 and described sample
It is respectively arranged with scale value in the outside wall surface of product enrichment room 4.
In this embodiment, the outside wall surface of first separation chamber 2, precipitation separation room 3 and example enrichment room 4 is respectively arranged with quarter
Angle value, facilitates product to carry out the guarantee of solution volume during separation and Extraction, enables product to be standardized operation according to program.
As shown in Figures 2 to 4, in one particular embodiment of the present invention, first separation chamber 2 cylindrically, and thereon
Portion arranges external screw thread, and meanwhile, first separation chamber 2 is connected with precipitation separation room 3 integral type, and first separation chamber 2 capacity is about 450 milliliters,
Overall diameter is set to 8 centimetres, and height is 9 centimetres, and upper cover 1 the most cylindrically, and has the external screw thread with first separation chamber 2 and matches
Female thread, the interior diameter of upper cover 1 is 8 centimetres, is highly arranged on 1.5 centimetres to 2.0 centimetres；Precipitation separation room 3 in cone,
2.5 mm wide circular platforms are being set with junction, first separation chamber 2, in order to carry the second drainage screen 8, the appearance of precipitation separation room 3
Amassing and be about 240 milliliters, and precipitation separation room 3 upper port overall diameter is 8.0 centimetres, the overall diameter of lower port is 3.0 centimetres, separates
The height of mud chamber 3 is 5.8 centimetres；Example enrichment room 4 is in cone, and top is connected, at the two with precipitation separation room 3 integral type
Junction be again provided with the circular platform of 2.5 mm wides, in order to carry the 3rd drainage screen 9, the volume of example enrichment room 4 is about
Being 7.0 milliliters, the overall diameter of the upper port of example enrichment room 4 is 3.0 centimetres, and the overall diameter of lower port is 0.8 centimetre, and sample is rich
The height of collection room 4 is 2.2 centimetres；Sample collection portion 6 cylindrically, and upper/lower terminal opening, top connects example enrichment room 4,
Lower connecting strap has the sample divider 5 of half female thread, sample collection portion 6 volume to be about 12 milliliters, and overall diameter is 0.8 li
Rice, the height of sample collection portion 6 is about 1.5 centimetres；Cylindrically, its upper end arranges opening to sample divider 5, bottom lock,
Capacity is about 0.7 milliliter, and the height of sample divider 5 is about 1.4 centimetres, and the interior diameter of sample divider 5 is 0.8 centimetre,
And the female thread 51 of 0.5 cm height is set on the top of sample divider 5, making sample divider is half screw-type cylinder
Shape, and be spirally connected with sample collection portion 6, meanwhile, make the smooth inner wall face that the lower inner wall face of sample divider 5 arranges 1.0 centimetres
For sample collection area, the capacity of sample collection area is about 0.5 milliliter；Support 10 is cylindrical, and the external diameter of support 10 is 8 centimetres,
Its side is totally-enclosed or fractional open, and bottom, for open, facilitates the dismounting of sample divider 5, the top of support 10 with just separate
Bottom, room 2 connects, and the height of support 10 is more than precipitation separation room 3, sample collection room and sample collection portion 6 three's total height sum
2 to 3 millimeters；It addition, the first drainage screen 7 makes recessed net with acid metal, and metal edges is set at the periphery of the first drainage screen 7
Frame, the mesh mesh number of the first drainage screen 7 is 20 to 30 mesh, and a diameter of 8 centimetres of the barrier of the first drainage screen 7, concave surface height is 1.5
Centimetre, a diameter of 8.4 centimetres of metal edge frame；Second drainage screen 8 acidproof metal or nylon makes plain net, and its periphery is arranged
Metal edge frame, the overall diameter of metal edge frame is 7.9 centimetres, a diameter of 7.8 centimetres of the barrier of the second drainage screen 8, the second drainage screen 8
Mesh mesh number be 40 to 50 mesh；3rd drainage screen 9 acidproof metal or nylon makes plain net, and its periphery arranges metal edges
Frame, frame overall diameter is 2.9 centimetres, the 3rd a diameter of 2.8 centimetres of drainage screen 9 district, the mesh mesh number of the 3rd drainage screen be 50 to
70 mesh.
In this specific embodiment, on the one hand, make product facility simple in construction, easily to its normalizing operation, carry meanwhile
The high acquisition purity to separator, meanwhile, convenient to the cleaning after the use of each drainage screen.
The present invention provide metacercaria of liver flukes separation equipment, through first separation chamber, precipitation separation room, example enrichment room, sample
The optimization of product catcher and support is integrated, then through the first drainage screen, the second drainage screen and the setting of the 3rd drainage screen, makes digestion
After tissue digest through the filtration precipitation concentration of three times, instead of gradation of the prior art washing, it is to avoid repeatedly wash
The loss of bladder worm when washing, simultaneously, moreover it is possible to effectively remove the bulk impurity after tissue digestion, can be 150 microns by size
Bladder worm be fully enriched in sample divider, and then carry out disposable microscopy so that it is easily carry out according to standardization, reduce people
Power material resources, reduce cost, improve the service efficiency of product.
The using method flow chart of the metacercaria of liver flukes separation equipment that Fig. 5 provides for another embodiment of the present invention；Such as Fig. 5 institute
Show, the using method of the metacercaria of liver flukes separation equipment that the embodiment of second aspect present invention provides, including:
Step S1, opens upper cover, adds about 300mL normal saline or clean water, back-outs sample divider, makes liquid complete
It is full of bottom separation equipment, does not stay bubble, tighten sample divider；
Step S2, supplementary normal saline or clean water are to 300mL scale value position, by postdigestive tissue digest about
300mL pours in the first drainage screen, makes liquid naturally fall, and after addition, liquid level is located approximately at 600mL scale value position；
Step S3, covers tightly upper cover, and vertical standing 30 minutes to 40 minutes, at the beginning of tapping gently according to horizontal direction during this period
Separation chamber's part 2 to 3 times, makes to pass under vibrations, and auxiliary bladder worm passes through the second drainage screen and the 3rd drainage screen；
Step S4, keeps upper cover for covering tightly state, turns round lower sample divider, and the bladder worm centralized collection after precipitation is received at sample
The latter half of storage；
Step S5, draws and is totally separated thing to microscope slide or little plate, low power microscope or dissection Microscopic observation bladder worm；
Step S6, opens upper cover, outwells liquid, fully clean separation equipment after use, carry out after drying
Next time uses.
Further, after step S2, step S201 is also included, it is simply that after step S2 before step S3, mention ground floor mistake
Filter screen, discards postdigestive tissue digest residue.
Further, in step sl, when adding solution, sample collection portion liquid to be completely filled with, do not stay bubble, permissible
Rotate and loosen half screw-type sample divider, make liquid naturally fall this step.Treat that liquid is completely filled with separation equipment
The latter half, tightens sample divider during especially half screw-type sample divider.Liquid make-up is to 300mL scale value position
Put.
In step s 4, the liquid volume that sample divider is gathered in the crops is about 500 microlitres.
It should be noted that when by this separation equipment separation and Extraction, it should be noted that (1) tissue to be checked such as the flesh of fish
Take 30 to 50 grams, add digestion liquid measure and be up to 300mL；The foreign material such as fishbone, fish skin and fish scale should be removed before digestion, clean dry
Only.(2) purpose being previously added 300mL normal saline or clean water is full segregation apparatus and dilution digest, makes to separate
Journey is smoothed out, and reduces impurity in separator, and beneficially microscope is observed.The liquid of separation equipment bottom is full required complete
Entirely, not staying air pocket, especially " half screw-type sample divider " position, otherwise can affect bladder worm and naturally sink concentrated
Journey.
Use this separation equipment that metacercaria of liver flukes in postdigestive tissue digest is carried out separation and Extraction, it is thus achieved that separation
Thing high purity, it is possible to disposably complete microscopy, meanwhile, carry out operation with this separation equipment and decrease manpower and materials, reduce
The running cost of product, it addition, eliminate the process of multistep washing, makes the operating time be greatly reduced, improves product separation
Efficiency, and then improve the serviceability of product.
Last it is noted that various embodiments above is only in order to illustrate technical scheme, it is not intended to limit；To the greatest extent
The present invention has been described in detail by pipe with reference to foregoing embodiments, it will be understood by those within the art that: it depends on
So the technical scheme described in foregoing embodiments can be modified, or the most some or all of technical characteristic is entered
Row equivalent；And these amendments or replacement, do not make the essence of appropriate technical solution depart from various embodiments of the present invention technology
The scope of scheme.
Claims (10)
1. a metacercaria of liver flukes separation equipment, it is characterised in that including:
Just separation chamber, the described just two ends of separation chamber offer respectively and are provided with at upper shed and under shed, and described upper shed
First drainage screen；
Upper cover, described upper cover is with described just separation chamber is dismountable is connected, and can seal described upper shed；
Precipitation separation room, the upper end of described precipitation separation room is connected with the lower end of described just separation chamber, and with described under shed
It is connected, and described lower opening is provided with the second drainage screen；
Example enrichment room, the upper end of described example enrichment room is connected with the lower end of described precipitation separation room and connects, and described
Example enrichment room is provided with the 3rd drainage screen with the connectivity part of described precipitation separation room；
Sample divider, the upper end of described sample divider has an opening, and described opening part with under described example enrichment room
Hold dismountable connection and connect；With
Support, described support is connected with the lower end of described just separation chamber, and metacercaria of liver flukes separation equipment can be supported on water
In plane, and between the supporting surface at described sample divider and described support place, there is gap.
Metacercaria of liver flukes separation equipment the most according to claim 1, it is characterised in that
Described just separation chamber is tubular, and described precipitation separation room and described example enrichment room are the most tapered.
Metacercaria of liver flukes separation equipment the most according to claim 1, it is characterised in that
Described first drainage screen is recessed net, and the metal material that described first drainage screen is acidproof is made, and in described first mistake
The periphery of filter screen is provided with metal edge frame.
Metacercaria of liver flukes separation equipment the most according to claim 1, it is characterised in that
Described second drainage screen and described 3rd drainage screen are respectively plain net, and described second drainage screen and described 3rd drainage screen
It is respectively the most acidproof metal material or nylon is made, and set respectively at the periphery of described second drainage screen and described 3rd drainage screen
It is equipped with metal edge frame.
Metacercaria of liver flukes separation equipment the most according to any one of claim 1 to 4, it is characterised in that also include sample
Collection portion；
The mating shapes of the shape of described sample collection portion and described sample divider, and the upper end of described sample collection portion with
The lower end of described example enrichment room is connected and connects, the lower end of described sample collection portion and the opening part of described sample divider
Dismountable connection also connects.
Metacercaria of liver flukes separation equipment the most according to claim 5, it is characterised in that
The opening part of described sample divider is provided with female thread, and the lower end of described sample collection portion is provided with and described female thread
The external screw thread matched.
Metacercaria of liver flukes separation equipment the most according to any one of claim 1 to 4, it is characterised in that
Be provided with external screw thread at the upper shed of described just separation chamber, described on cover and be provided with and match with described external screw thread in
Screw thread；Or, be provided with Access Division at the upper shed of described just separation chamber, described on cover to be provided with and match with described Access Division
Buckle.
Metacercaria of liver flukes separation equipment the most according to any one of claim 1 to 4, it is characterised in that
It is respectively arranged with scale value in the outside wall surface of described just separation chamber, described precipitation separation room and described example enrichment room.
9. the using method of a metacercaria of liver flukes separation equipment, it is characterised in that including:
Step S1, opens upper cover, adds about 300mL normal saline or clean water, back-outs sample divider, makes liquid be completely filled with
Bottom separation equipment, do not stay bubble, tighten sample divider；
Step S2, supplementary normal saline or clean water are to 300mL scale value position, by postdigestive tissue digest about 300mL
Pouring in the first drainage screen, make liquid naturally fall, after addition, liquid level is located approximately at 600mL scale value position；
Step S3, covers tightly upper cover, vertical standing 30 minutes to 40 minutes, taps just separation during this period gently according to horizontal direction
Room part 2 to 3 times, makes to pass under vibrations, and auxiliary bladder worm passes through the second drainage screen and the 3rd drainage screen；
Step S4, keeps upper cover for covering tightly state, turns round lower sample divider, and the bladder worm centralized collection after precipitation is at sample divider
The latter half；
Step S5, draws and is totally separated thing to microscope slide or little plate, low power microscope or dissection Microscopic observation bladder worm；
Step S6, opens upper cover, outwells liquid, fully clean separation equipment after use, carry out next time after drying
Use.
The using method of metacercaria of liver flukes separation equipment the most according to claim 9, it is characterised in that
After step S2, also include step S201, mention ground floor drainage screen, discard postdigestive tissue digest residue.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201610419911.7A CN106124713B (en) | 2016-06-14 | 2016-06-14 | The application method of metacercaria of liver flukes separation equipment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610419911.7A CN106124713B (en) | 2016-06-14 | 2016-06-14 | The application method of metacercaria of liver flukes separation equipment |
Publications (2)
| Publication Number | Publication Date |
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| CN106124713A true CN106124713A (en) | 2016-11-16 |
| CN106124713B CN106124713B (en) | 2019-01-29 |
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| CN201610419911.7A Expired - Fee Related CN106124713B (en) | 2016-06-14 | 2016-06-14 | The application method of metacercaria of liver flukes separation equipment |
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Also Published As
| Publication number | Publication date |
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| CN106124713B (en) | 2019-01-29 |
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