Detailed description of the invention
Below in conjunction with embodiment, the specific embodiment of the present invention is elaborated.
The present invention can be provided by following examples in concrete enforcement.
Embodiment 1
The present invention, in concrete enforcement, is made up of the raw material of following weight percent meter: Radix Glycyrrhizae 2-3%, Rhizoma Atractylodis Macrocephalae 2-3%, Pseudobulbus Bletillae (Rhizoma Bletillae) 8-12%, Semen Ginkgo 2-3%, Radix Panacis Quinquefolii 4-6%, Radix Salviae Miltiorrhizae 12-14%, Rhizoma Zingiberis Recens 15-20%, colloidal tio 2 (TiO2) 1.9-2.1% of mass concentration 10%, glycerol 7.5-8.5%, ethanol 11-13%, methyl parahydroxybenzoate 0.09-0.11%, surplus is deionized water, wherein, first by Radix Glycyrrhizae, the Rhizoma Atractylodis Macrocephalae, the Pseudobulbus Bletillae (Rhizoma Bletillae), Semen Ginkgo, Radix Panacis Quinquefolii, Radix Salviae Miltiorrhizae, Rhizoma Zingiberis Recens mixes, and with distilled water submergence 30-60 minute, intense fire is fried boiling, slow fire boils 20-30 minute again, filters, and obtains first time filtrate, medicinal residues add water to submerge again, and intense fire is fried boiling, then slow fire boils 20-30 minute, filter, obtain second time filtrate, merge twice filtrate, 1 ︰ 1 adds the ethanol of mass concentration 90% in filtrate by volume, 4 DEG C of standing 24h, collect supernatant, filter, reclaim ethanol ,-80 DEG C of pre-coolings, vacuum lyophilization, obtains lyophilized products, with the colloidal tio 2 of mass concentration 10%, glycerol, ethanol, methyl parahydroxybenzoate, deionized water mixes.
Embodiment 2
The present invention, in concrete enforcement, also can be made up of the raw material of following weight percent meter: Radix Glycyrrhizae 0.5%, the Rhizoma Atractylodis Macrocephalae 0.5%, the Pseudobulbus Bletillae (Rhizoma Bletillae) 2%, Semen Ginkgo 0.5%, Radix Panacis Quinquefolii 1%, Radix Salviae Miltiorrhizae 10%, Rhizoma Zingiberis Recens 10%, the colloidal tio 2 2% of mass concentration 10%, glycerol 8%, ethanol 12%, methyl parahydroxybenzoate 0.1%, surplus is deionized water, wherein, first by Radix Glycyrrhizae, the Rhizoma Atractylodis Macrocephalae, the Pseudobulbus Bletillae (Rhizoma Bletillae), Semen Ginkgo, Radix Panacis Quinquefolii, Radix Salviae Miltiorrhizae, Rhizoma Zingiberis Recens mixes, and with distilled water submergence 30-60 minute, intense fire is fried boiling, slow fire boils 20-30 minute again, filters, and obtains first time filtrate, medicinal residues add water to submerge again, and intense fire is fried boiling, then slow fire boils 20-30 minute, filter, obtain second time filtrate, merge twice filtrate, 1 ︰ 1 adds the ethanol of mass concentration 90% in filtrate by volume, 4 DEG C of standing 24h, collect supernatant, filter, reclaim ethanol ,-80 DEG C of pre-coolings, vacuum lyophilization, obtains lyophilized products, with the colloidal tio 2 of mass concentration 10%, glycerol, ethanol, methyl parahydroxybenzoate, deionized water mixes.
Embodiment 3
The present invention, in concrete enforcement, also can be made up of the raw material of following weight percent meter: Radix Glycyrrhizae 1%, the Rhizoma Atractylodis Macrocephalae 1%, the Pseudobulbus Bletillae (Rhizoma Bletillae) 5%, Semen Ginkgo 1%, Radix Panacis Quinquefolii 3%, Radix Salviae Miltiorrhizae 13%, Rhizoma Zingiberis Recens 15%, the colloidal tio 2 1.8% of mass concentration 10%, glycerol 9%, ethanol 11%, methyl parahydroxybenzoate 0.12%, surplus is deionized water, wherein, first by Radix Glycyrrhizae, the Rhizoma Atractylodis Macrocephalae, the Pseudobulbus Bletillae (Rhizoma Bletillae), Semen Ginkgo, Radix Panacis Quinquefolii, Radix Salviae Miltiorrhizae, Rhizoma Zingiberis Recens mixes, and with distilled water submergence 30-60 minute, intense fire is fried boiling, slow fire boils 20-30 minute again, filters, and obtains first time filtrate, medicinal residues add water to submerge again, and intense fire is fried boiling, then slow fire boils 20-30 minute, filter, obtain second time filtrate, merge twice filtrate, 1 ︰ 1 adds the ethanol of mass concentration 90% in filtrate by volume, 4 DEG C of standing 24h, collect supernatant, filter, reclaim ethanol ,-80 DEG C of pre-coolings, vacuum lyophilization, obtains lyophilized products, with the colloidal tio 2 of mass concentration 10%, glycerol, ethanol, methyl parahydroxybenzoate, deionized water mixes.
Embodiment 4
The present invention, in concrete enforcement, also can be made up of the raw material of following weight percent meter: Radix Glycyrrhizae 2%, the Rhizoma Atractylodis Macrocephalae 2%, the Pseudobulbus Bletillae (Rhizoma Bletillae) 8%, Semen Ginkgo 2%, Radix Panacis Quinquefolii 7%, Radix Salviae Miltiorrhizae 15%, Rhizoma Zingiberis Recens 17%, the colloidal tio 2 2.2% of mass concentration 10%, glycerol 7%, ethanol 13%, methyl parahydroxybenzoate 0.09%, surplus is deionized water, wherein, first by Radix Glycyrrhizae, the Rhizoma Atractylodis Macrocephalae, the Pseudobulbus Bletillae (Rhizoma Bletillae), Semen Ginkgo, Radix Panacis Quinquefolii, Radix Salviae Miltiorrhizae, Rhizoma Zingiberis Recens mixes, and with distilled water submergence 30-60 minute, intense fire is fried boiling, slow fire boils 20-30 minute again, filters, and obtains first time filtrate, medicinal residues add water to submerge again, and intense fire is fried boiling, then slow fire boils 20-30 minute, filter, obtain second time filtrate, merge twice filtrate, 1 ︰ 1 adds the ethanol of mass concentration 90% in filtrate by volume, 4 DEG C of standing 24h, collect supernatant, filter, reclaim ethanol ,-80 DEG C of pre-coolings, vacuum lyophilization, obtains lyophilized products, with the colloidal tio 2 of mass concentration 10%, glycerol, ethanol, methyl parahydroxybenzoate, deionized water mixes.
Embodiment 5
The present invention, in concrete enforcement, also can be made up of the raw material of following weight percent meter: Radix Glycyrrhizae 4%, the Rhizoma Atractylodis Macrocephalae 4%, the Pseudobulbus Bletillae (Rhizoma Bletillae) 10%, Semen Ginkgo 4%, Radix Panacis Quinquefolii 9%, Radix Salviae Miltiorrhizae 17%, Rhizoma Zingiberis Recens 20%, the colloidal tio 2 2.1% of mass concentration 10%, glycerol 7.5%, ethanol 12%, methyl parahydroxybenzoate 0.11%, surplus is deionized water, wherein, first by Radix Glycyrrhizae, the Rhizoma Atractylodis Macrocephalae, the Pseudobulbus Bletillae (Rhizoma Bletillae), Semen Ginkgo, Radix Panacis Quinquefolii, Radix Salviae Miltiorrhizae, Rhizoma Zingiberis Recens mixes, and with distilled water submergence 30-60 minute, intense fire is fried boiling, slow fire boils 20-30 minute again, filters, and obtains first time filtrate, medicinal residues add water to submerge again, and intense fire is fried boiling, then slow fire boils 20-30 minute, filter, obtain second time filtrate, merge twice filtrate, 1 ︰ 1 adds the ethanol of mass concentration 90% in filtrate by volume, 4 DEG C of standing 24h, collect supernatant, filter, reclaim ethanol ,-80 DEG C of pre-coolings, vacuum lyophilization, obtains lyophilized products, with the colloidal tio 2 of mass concentration 10%, glycerol, ethanol, methyl parahydroxybenzoate, deionized water mixes.
According to the theory of constitution from the traditional Chinese medical science, the complexion of people, namely the situation of skin can embody the constitution status of human body.Body constitution is good, skin moisturizing, smooth, flexible; Body constitution is weak, skin is intense darkness without light pool.Chinese medicine is thought, " defending temperature boundary between muscles, fertile space between skin and muscles ", " lucid YANG spreading into the body surface ", " body fluid, profit skin " etc., and namely the pliable and tough gloss of skin depends on and defends temperature and to support and body fluid makes moist.If there is exopathogen to block, or the gas deficiency of YIN is weak, and clear sun is unable space between skin and muscles then, and skin is easy to be withered simple and unadorned, also easily makes body fluid and blood stasis in face, occurs skin dark stain.Radix Salviae Miltiorrhizae in the present invention, bitter, be slightly cold.GUIXIN, Liver Channel, have the merit of blood circulation promoting and blood stasis dispelling, removing heat from blood eliminating carbuncle, and " Mingyi Bielu " also says that it can nourish blood, in this case monarch drug; The Pseudobulbus Bletillae (Rhizoma Bletillae), bitter, sweet, puckery, be slightly cold, return lung, liver, stomach warp, have promoting blood circulation and hemostasis, the effect of detumescence and promoting granulation, " property of medicine opinion " says it: " control stagnation of pathogenic heat not disappear, flaccidity under main the moon, control Shao-bleb on face, make us flesh and slide." Rhizoma Zingiberis Recens, Xin Wen, returns lung, spleen, stomach warp, and can induce sweat cold expelling, warming middle-JIAO to arrest vomiting; Radix Panacis Quinquefolii, sweet, micro-hardship, cool, GUIXIN, lung, kidney channel, can boosting qi and nourishing yin, clearing away heat and promoting production of body fluid, and three had both helped monarch's blood circulation promoting and blood stasis dispelling, again can QI invigorating stomach function regulating, is ministerial drug altogether; The Rhizoma Atractylodis Macrocephalae, invigorating the spleen and benefiting QI, Semen Ginkgo, nourishing lung and kidney, helping inspiration to relieve asthma is assistant; Radix Glycyrrhizae, invigorating the spleen and benefiting QI, coordinating the actions of various ingredients in a prescription, for making.7 medicines are harmonious, and coordinate with other component, support mutually, gather the effect of eliminating evil, supplementing QI and nourishing YIN, moisturizing whitening of invigorating blood circulation altogether.And achieve very satisfied Advantageous Effects through actual tests, related tests data is as follows:
1. protective skin cream moisturizing degree and moisture preserving time detect
MMV (moisture measurement value) method detects the ability of MMV value reflection keratodermatitis Bound moisture.Adopt the hydration of CM825 type skin oil-water soda acid tester test skin.Its measuring principle is because horny layer contains a large amount of electrolyte, and the electrolyte existed in water has electric conductivity, and this phenomenon also produces at skin surface, and after test probe and contact skin, the change of electrical conductivity reflects the hydration status of skin.
Experimenter is healthy without dermopathic volunteer.Experimental condition is that the temperature and humidity of test period environment is constant, and temperature is 25 DEG C ± 2 DEG C, and relative humidity is 40% ~ 60%.
Choose healthy without dermopathic volunteer 10, test.Method of testing is: 1. determine left and right forearm 4 pieces of test zones, region area is 4cm × 4cm, tests the blank value in this region respectively.2. sample is coated on test zone, consumption is 2mg/cm
2.3. after using, 15 min, 30 min, 60 min, 90 min, 120 min test, and every block domain test 5 points, average.
The judgement schematics that moistening effect is evaluated: moisturizing rate/%=(P relatively
sample t=x-P
blank)/(P
mark t=x-P
blank) × 100
In formula:
P
sample t=xthe electrical conductivity of product after-use certain hour;
P
blank-be not coated with the electrical conductivity of sample;
P
mark t=xthe electrical conductivity of object of reference after-use certain hour
The moisturizing rate situation (%) that different time points protective skin cream and glycerol compare
Time (min) |
15 |
30 |
60 |
90 |
120 |
Protective skin cream |
0.68 |
0.55 |
0.69 |
0.55 |
0.5 |
Glycerol |
0.5 |
0.25 |
0.44 |
0.42 |
0.4 |
From above form, the moisturizing rate of protective skin cream is apparently higher than the moisturizing situation of glycerol.
2. protective skin cream Chinese medicine part detects the anti-aging effects of skin
Free radical is the intermediate product of biochemical reaction in vital movement process, with the aging relevant Biological Free Radicals of life mainly some oxygen containing free radicals, antioxidant can provide proton to peroxide radical, thus can suppress the autoxidizable chain reaction of lipid.Usually DPP H is made to put forward protogenic ability to check antioxidant.D P P H is a kind of very stable free radical, and its methanol solution has extremely strong absorbance at 517n m place, but when oxidized dose (A H) or free radical (R) reduce, its absorbance can reduce or disappear.Thus, the absorbance of sample at 517n m place can be utilized to judge whether sample has the ability of scavenging free radicals.
Get the Chinese medicinal powder of lyophilizing, be configured to the solution of 50mg/ml, 100mg/ml, 200mg/ml, 400mg/ml, 800mg/ml with PBS, respectively with 3. 8 ml methanol solutions, 1ml (DPPH) radical methanol solution (2 × 10
-4mol/L), be fully mixed evenly, after leaving standstill 30min, with spectrophotometric measurement 517nm place absorbance, and calculate its free radical scavenging activity with the formula.Free radical scavenging activity (%)=[(Ac-As/Ac)] × 100%
A s: the sample containing determinand is at the absorbance at 517nm place;
A c: not containing the absorbance of sample at 517nm place of determinand
The free radical scavenging activity (%) of the Chinese medicine extract of different volumes
Chinese medicine part (mg/ml) |
5 |
10 |
20 |
40 |
80 |
Clearance rate (%) |
10.1 |
13.8 |
31.5 |
63.3 |
80.6 |
As can be seen from the table, along with the increase of Chinese medicine volume, free radical scavenging activity also correspondingly increases.Illustrate that protective skin cream has good defying age ability.
3. protective skin cream whitening effect detects
Select each 15 of the men and women volunteer that the colour of skin is darker, carry out smearing of morning and evening interval 12h to the protective skin cream of right face containing Chinese medicine whitening composition after clean of morning and evening, left face in contrast, only smears the matrix components of table two, surrounding on probation, fills in evaluation table on probation according to sensory evaluation.Best result 4 is divided into Be very effective, is minimumly divided into 1, and contrast effect is not obvious.Card side is utilized to add up in first week (table three), second week (table four), the 3rd week (table five), 4th week (table six) respectively.
First week post-evaluation effect smeared by table three
Evaluation effect |
1 |
2 |
3 |
4 |
Left face |
15 |
0 |
0 |
0 |
Right face |
13 |
1 |
1 |
0 |
Zero difference
Second week post-evaluation effect smeared by table four
Evaluation effect |
1 |
2 |
3 |
4 |
Left face |
14 |
1 |
0 |
0 |
Right face |
3 |
11 |
1 |
0 |
P﹤0.05
The 3rd week post-evaluation effect smeared by table five
Evaluation effect |
1 |
2 |
3 |
4 |
Left face |
14 |
1 |
0 |
0 |
Right face |
1 |
4 |
10 |
0 |
P﹤0.05
4th week post-evaluation effect smeared by table six
Evaluation effect |
1 |
2 |
3 |
4 |
Left face |
13 |
2 |
0 |
0 |
Right face |
0 |
4 |
2 |
9 |
P﹤0.05
Smear week age and carry out paired observation, the half of face in left and right does not obviously contrast, but along with the prolongation of time, use two weeks, three weeks, after surrounding, there is obvious speckle fading effect (P ﹤ 0.05) in right face.Above data illustrate that the present invention has effect of whitening.
In the present invention, Radix Glycyrrhizae can eliminate free radical, promotes the generation of collagen protein, the division of check melanin cell; The Rhizoma Atractylodis Macrocephalae mainly adopts the extract of dry root, can promote the generation of collagen-type IV, and the melanocyte of suppression is active, has good water retaining function; The Pseudobulbus Bletillae (Rhizoma Bletillae) adopts the extract of dry tuber, the activity of check melanin cell, eliminates free radical, the generation of the lipid peroxidation of suppression; Folium Ginkgo eliminates free radical, the activity of the tryrosinase of suppression, promotes the generation of tight junction protein; Radix Panacis Quinquefolii mainly with the extract of its dry root, has antioxidation, can promote the generation of elastin laminin, eliminate superoxide radical, promote the activity of dermal fibroblasts, increase the growing amount of elastin laminin, having good moisture holding capacity, is the good wetting agents of cosmetics; Radix Salviae Miltiorrhizae adopts the extract of its dry root, eliminates superoxide radical, promotes fibroblastic increment, promotes collagen protein and hyaluronic generation; The extract of the race stem that Rhizoma Zingiberis Recens is fresh or dry, suppresses elastase activity, eliminates superoxide radical, strengthens the activity of skin, can be used as anatrophic and wetting agent.There is fine practicality, be the skin care item of the anti-ageing moisturizing of a kind of efficient whitening, have good economic and social benefit.