CN104797704A - 重组细胞以及β-水芹烯的生产方法 - Google Patents

重组细胞以及β-水芹烯的生产方法 Download PDF

Info

Publication number
CN104797704A
CN104797704A CN201380060882.4A CN201380060882A CN104797704A CN 104797704 A CN104797704 A CN 104797704A CN 201380060882 A CN201380060882 A CN 201380060882A CN 104797704 A CN104797704 A CN 104797704A
Authority
CN
China
Prior art keywords
phellandrene
amino acid
acid sequence
nucleic acid
recombinant cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201380060882.4A
Other languages
English (en)
Chinese (zh)
Inventor
古谷昌弘
上西章太
岩佐航一郎
郡悌之
高桥征司
下山武文
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Tohoku University NUC
Sekisui Chemical Co Ltd
Original Assignee
Tohoku University NUC
Sekisui Chemical Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Tohoku University NUC, Sekisui Chemical Co Ltd filed Critical Tohoku University NUC
Publication of CN104797704A publication Critical patent/CN104797704A/zh
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/007—Preparation of hydrocarbons or halogenated hydrocarbons containing one or more isoprene units, i.e. terpenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/002—Preparation of hydrocarbons or halogenated hydrocarbons cyclic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y205/00—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5)
    • C12Y205/01—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5) transferring alkyl or aryl groups, other than methyl groups (2.5.1)
    • C12Y205/01001—Dimethylallyltranstransferase (2.5.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y205/00—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5)
    • C12Y205/01—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5) transferring alkyl or aryl groups, other than methyl groups (2.5.1)
    • C12Y205/01028—Dimethylallylcistransferase (2.5.1.28)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/03—Carbon-oxygen lyases (4.2) acting on phosphates (4.2.3)
    • C12Y402/03051—Beta-phellandrene synthase (neryl-diphosphate-cyclizing) (4.2.3.51)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201380060882.4A 2012-09-21 2013-09-19 重组细胞以及β-水芹烯的生产方法 Pending CN104797704A (zh)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
JP2012-208292 2012-09-21
JP2012208292 2012-09-21
JP2013-119612 2013-06-06
JP2013119612A JP2014076042A (ja) 2012-09-21 2013-06-06 組換え細胞、並びに、β−フェランドレンの生産方法
PCT/JP2013/075282 WO2014046174A1 (ja) 2012-09-21 2013-09-19 組換え細胞、並びに、β－フェランドレンの生産方法

Publications (1)

Publication Number Publication Date
CN104797704A true CN104797704A (zh) 2015-07-22

Family

ID=50341478

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201380060882.4A Pending CN104797704A (zh) 2012-09-21 2013-09-19 重组细胞以及β-水芹烯的生产方法

Country Status (6)

Country Link
US (1) US9617563B2 (https=)
EP (1) EP2899263A4 (https=)
JP (1) JP2014076042A (https=)
KR (1) KR20150059649A (https=)
CN (1) CN104797704A (https=)
WO (1) WO2014046174A1 (https=)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108138162A (zh) * 2015-11-30 2018-06-08 积水化学工业株式会社 重组细胞，重组细胞的制造方法以及有机化合物的生产方法
CN112257225A (zh) * 2020-09-16 2021-01-22 中国科学院地理科学与资源研究所 一种适用于高寒草地生态系统的npp计算方法
CN114350713A (zh) * 2021-12-30 2022-04-15 中国林业科学研究院亚热带林业研究所 山鸡椒水芹烯合成酶在制备萜烯类化合物及包含萜烯类化合物的产品中的应用

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9951354B2 (en) 2012-02-06 2018-04-24 The Regents Of The University Of California Production of β-phellandrene using genetically engineered cyanobacteria
JP6145937B2 (ja) * 2013-10-21 2017-06-14 国立大学法人名古屋大学 βフェランドレン重合体、その製造方法及び成形品
CN105658685B (zh) * 2013-10-21 2019-04-05 国立大学法人名古屋大学 β水芹烯聚合物、其制造方法及成形品
US20180023098A1 (en) * 2015-02-13 2018-01-25 Sekisui Chemical Co., Ltd. Nucleic acid, fusion protein, recombined cell, and isoprene or cyclic terpene production method
CN107815424B (zh) * 2016-09-12 2021-03-05 华东理工大学 一种产柠檬烯的解脂耶氏酵母基因工程菌及其应用
JP6341439B2 (ja) * 2017-03-21 2018-06-13 国立大学法人名古屋大学 βフェランドレン重合体、その製造方法及び成形品
CN109810999B (zh) * 2019-03-11 2023-03-24 湖北工业大学 一种利用微生物发酵生产橙花醇的方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2000145482A (ja) * 1998-11-11 2000-05-26 Asmo Co Ltd スロットル制御装置
JP2005500805A (ja) * 2000-09-01 2005-01-13 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー 環式テルペノイド類の製造

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2000245482A (ja) * 1999-03-05 2000-09-12 Sozoteki Seibutsu Kogaku Kenkyusho:Kk モノテルペンの生産方法及びそのための微生物
US6660507B2 (en) 2000-09-01 2003-12-09 E. I. Du Pont De Nemours And Company Genes involved in isoprenoid compound production
US7172886B2 (en) * 2001-12-06 2007-02-06 The Regents Of The University Of California Biosynthesis of isopentenyl pyrophosphate
AU2008299020A1 (en) * 2007-09-11 2009-03-19 Sapphire Energy, Inc. Molecule production by photosynthetic organisms
AU2008305655B2 (en) * 2007-09-20 2013-10-31 Amyris, Inc. Production of isoprenoids
US9951354B2 (en) 2012-02-06 2018-04-24 The Regents Of The University Of California Production of β-phellandrene using genetically engineered cyanobacteria

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2000145482A (ja) * 1998-11-11 2000-05-26 Asmo Co Ltd スロットル制御装置
JP2005500805A (ja) * 2000-09-01 2005-01-13 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー 環式テルペノイド類の製造

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
DEMISSIE ZA,: "Cloning and functional characterization of synthase from lavandula angustifolia", 《PLANTA》 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN108138162A (zh) * 2015-11-30 2018-06-08 积水化学工业株式会社 重组细胞，重组细胞的制造方法以及有机化合物的生产方法
CN112257225A (zh) * 2020-09-16 2021-01-22 中国科学院地理科学与资源研究所 一种适用于高寒草地生态系统的npp计算方法
CN112257225B (zh) * 2020-09-16 2023-07-14 中国科学院地理科学与资源研究所 一种适用于高寒草地生态系统的npp计算方法
CN114350713A (zh) * 2021-12-30 2022-04-15 中国林业科学研究院亚热带林业研究所 山鸡椒水芹烯合成酶在制备萜烯类化合物及包含萜烯类化合物的产品中的应用

Also Published As

Publication number Publication date
EP2899263A4 (en) 2016-04-13
JP2014076042A (ja) 2014-05-01
WO2014046174A1 (ja) 2014-03-27
US9617563B2 (en) 2017-04-11
US20150218589A1 (en) 2015-08-06
EP2899263A1 (en) 2015-07-29
KR20150059649A (ko) 2015-06-01

Similar Documents

Publication Publication Date Title
CN104797704A (zh) 重组细胞以及β-水芹烯的生产方法
TWI427149B (zh) 使用ｄｘｐ及ｍｖａ途徑之改良之異戊二烯製造
CA2886137C (en) Recombinant cell, and method for producing isoprene
CN105008514A (zh) 用于生物生产异戊二烯的组合物和方法
US9695447B2 (en) Recombinant cell and method for producing isoprene
EP3587572A1 (en) Recombinant cell, method for producing recombinant cell, and method for producing isoprene or terpene
JP7487099B2 (ja) レバウジオシドの高効率生成のためのエンドウ（ｐｉｓｕｍ ｓａｔｉｖｕｍ）カウレンオキシダーゼ
EP3574105B1 (en) Co-production of a sesquiterpene and a carotenoid
EP3385378A1 (en) Recombinant cell, method for producing recombinant cell, and method for producing organic compound
JP6470532B2 (ja) 組換え細胞、並びに、有機化合物の生産方法
JP2015181428A (ja) 組換え細胞、並びに、環式モノテルペンの生産方法
CN113260699A (zh) 用于高效生成瑞鲍迪苷的甜叶菊异贝壳杉烯酸羟化酶变体
JP2022150299A (ja) イソプレノイド類産生能を有する遺伝子改変微生物およびこれを用いたイソプレノイド類製造方法
JP2017055702A (ja) 組換え細胞、組換え細胞の製造方法、並びに、ショウブノールの生産方法
AU2020268897A1 (en) Kaurenoic acid 13-hydroxylase (KAH) variants and uses thereof
HK40017319B (en) Co-production of a sesquiterpene and a carotenoid
HK40017319A (en) Co-production of a sesquiterpene and a carotenoid
HK1196141B (en) Production of acetyl-coenzyme a derived isoprenoids
HK1196141A (en) Production of acetyl-coenzyme a derived isoprenoids

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication
RJ01 Rejection of invention patent application after publication

Application publication date: 20150722