CN104784684A - 一种鱼类烂鳃病病原约氏黄杆菌减毒活疫苗及构建方法 - Google Patents
一种鱼类烂鳃病病原约氏黄杆菌减毒活疫苗及构建方法 Download PDFInfo
- Publication number
- CN104784684A CN104784684A CN201510121169.7A CN201510121169A CN104784684A CN 104784684 A CN104784684 A CN 104784684A CN 201510121169 A CN201510121169 A CN 201510121169A CN 104784684 A CN104784684 A CN 104784684A
- Authority
- CN
- China
- Prior art keywords
- yue shi
- flavobacterium
- live vaccine
- shi flavobacterium
- attenuated live
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 229960005486 vaccine Drugs 0.000 title claims abstract description 41
- 230000002238 attenuated effect Effects 0.000 title claims abstract description 31
- 201000010099 disease Diseases 0.000 title claims abstract description 21
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title claims abstract description 21
- 238000010276 construction Methods 0.000 title claims abstract description 13
- 241000605108 Flavobacterium johnsoniae Species 0.000 title abstract description 9
- 230000001717 pathogenic effect Effects 0.000 title abstract 2
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 claims abstract description 30
- 241000251468 Actinopterygii Species 0.000 claims abstract description 27
- 230000001580 bacterial effect Effects 0.000 claims abstract description 26
- 229960005322 streptomycin Drugs 0.000 claims abstract description 15
- 241000589565 Flavobacterium Species 0.000 claims description 81
- 239000001963 growth medium Substances 0.000 claims description 10
- 238000002360 preparation method Methods 0.000 claims description 9
- 238000000034 method Methods 0.000 claims description 8
- 101150098466 rpsL gene Proteins 0.000 claims description 6
- 108090000623 proteins and genes Proteins 0.000 claims description 5
- 102000004169 proteins and genes Human genes 0.000 claims description 5
- 239000004475 Arginine Substances 0.000 claims description 3
- 108020004705 Codon Proteins 0.000 claims description 3
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 claims description 3
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 claims description 3
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 claims description 3
- 230000002265 prevention Effects 0.000 claims description 2
- 241000252230 Ctenopharyngodon idella Species 0.000 abstract description 19
- 230000001681 protective effect Effects 0.000 abstract description 8
- 238000002347 injection Methods 0.000 abstract description 7
- 239000007924 injection Substances 0.000 abstract description 7
- 230000003053 immunization Effects 0.000 abstract description 6
- 230000006698 induction Effects 0.000 abstract description 3
- 238000002791 soaking Methods 0.000 abstract description 3
- 238000002649 immunization Methods 0.000 abstract description 2
- 241000404975 Synchiropus splendidus Species 0.000 abstract 2
- 230000035772 mutation Effects 0.000 abstract 1
- 230000001988 toxicity Effects 0.000 abstract 1
- 231100000419 toxicity Toxicity 0.000 abstract 1
- 230000036039 immunity Effects 0.000 description 16
- 230000000694 effects Effects 0.000 description 12
- 210000002816 gill Anatomy 0.000 description 12
- 238000007654 immersion Methods 0.000 description 9
- 208000015181 infectious disease Diseases 0.000 description 8
- 210000004185 liver Anatomy 0.000 description 7
- 238000012360 testing method Methods 0.000 description 7
- 210000001519 tissue Anatomy 0.000 description 7
- 239000012137 tryptone Substances 0.000 description 6
- 230000001018 virulence Effects 0.000 description 6
- 241000264847 Siniperca chuatsi Species 0.000 description 5
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 5
- 210000003734 kidney Anatomy 0.000 description 5
- 239000002609 medium Substances 0.000 description 5
- 231100000350 mutagenesis Toxicity 0.000 description 5
- 239000000047 product Substances 0.000 description 5
- 210000000952 spleen Anatomy 0.000 description 5
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 4
- 238000009360 aquaculture Methods 0.000 description 4
- 244000144974 aquaculture Species 0.000 description 4
- 229940031551 inactivated vaccine Drugs 0.000 description 4
- 238000011534 incubation Methods 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 108010010803 Gelatin Proteins 0.000 description 3
- 206010018910 Haemolysis Diseases 0.000 description 3
- 229920002472 Starch Polymers 0.000 description 3
- 239000005018 casein Substances 0.000 description 3
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 3
- 235000021240 caseins Nutrition 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 230000003203 everyday effect Effects 0.000 description 3
- 239000012530 fluid Substances 0.000 description 3
- 239000008273 gelatin Substances 0.000 description 3
- 229920000159 gelatin Polymers 0.000 description 3
- 235000019322 gelatine Nutrition 0.000 description 3
- 235000011852 gelatine desserts Nutrition 0.000 description 3
- 230000008588 hemolysis Effects 0.000 description 3
- 230000002949 hemolytic effect Effects 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 231100000614 poison Toxicity 0.000 description 3
- 235000018102 proteins Nutrition 0.000 description 3
- 235000020183 skimmed milk Nutrition 0.000 description 3
- 235000019698 starch Nutrition 0.000 description 3
- 239000008107 starch Substances 0.000 description 3
- 239000003440 toxic substance Substances 0.000 description 3
- 102000002322 Egg Proteins Human genes 0.000 description 2
- 108010000912 Egg Proteins Proteins 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 101150062031 L gene Proteins 0.000 description 2
- 108010014251 Muramidase Proteins 0.000 description 2
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 2
- 241001494479 Pecora Species 0.000 description 2
- 108010046334 Urease Proteins 0.000 description 2
- 239000004202 carbamide Substances 0.000 description 2
- 235000013877 carbamide Nutrition 0.000 description 2
- 235000013345 egg yolk Nutrition 0.000 description 2
- 210000002969 egg yolk Anatomy 0.000 description 2
- 229940088598 enzyme Drugs 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 239000000706 filtrate Substances 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- 210000004943 gill filament Anatomy 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 231100000636 lethal dose Toxicity 0.000 description 2
- 239000013642 negative control Substances 0.000 description 2
- 230000007918 pathogenicity Effects 0.000 description 2
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 2
- 229920000053 polysorbate 80 Polymers 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 229940041022 streptomycins Drugs 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 208000024891 symptom Diseases 0.000 description 2
- IIZPXYDJLKNOIY-JXPKJXOSSA-N 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCC\C=C/C\C=C/C\C=C/C\C=C/CCCCC IIZPXYDJLKNOIY-JXPKJXOSSA-N 0.000 description 1
- 108020004465 16S ribosomal RNA Proteins 0.000 description 1
- ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 239000004382 Amylase Substances 0.000 description 1
- 102000013142 Amylases Human genes 0.000 description 1
- 108010065511 Amylases Proteins 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 102000013382 Gelatinases Human genes 0.000 description 1
- 108010026132 Gelatinases Proteins 0.000 description 1
- 239000004367 Lipase Substances 0.000 description 1
- 102000004882 Lipase Human genes 0.000 description 1
- 108090001060 Lipase Proteins 0.000 description 1
- 102000016943 Muramidase Human genes 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 235000019418 amylase Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- -1 casease Proteins 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000003086 colorant Substances 0.000 description 1
- 230000007123 defense Effects 0.000 description 1
- 239000000645 desinfectant Substances 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000006806 disease prevention Effects 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000003640 drug residue Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 230000014509 gene expression Effects 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 229910052740 iodine Inorganic materials 0.000 description 1
- 239000011630 iodine Substances 0.000 description 1
- 235000010445 lecithin Nutrition 0.000 description 1
- 239000000787 lecithin Substances 0.000 description 1
- 229940067606 lecithin Drugs 0.000 description 1
- 235000019421 lipase Nutrition 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000004325 lysozyme Substances 0.000 description 1
- 229960000274 lysozyme Drugs 0.000 description 1
- 235000010335 lysozyme Nutrition 0.000 description 1
- 206010025482 malaise Diseases 0.000 description 1
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 1
- 229910052753 mercury Inorganic materials 0.000 description 1
- 239000012982 microporous membrane Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 210000003097 mucus Anatomy 0.000 description 1
- 239000002773 nucleotide Substances 0.000 description 1
- 125000003729 nucleotide group Chemical group 0.000 description 1
- 230000003071 parasitic effect Effects 0.000 description 1
- 230000002085 persistent effect Effects 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N phenol group Chemical group C1(=CC=CC=C1)O ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 238000004080 punching Methods 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 102220025223 rs199476352 Human genes 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 229940031626 subunit vaccine Drugs 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Landscapes
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
Description
Claims (8)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201510121169.7A CN104784684B (zh) | 2015-03-19 | 2015-03-19 | 一种鱼类烂鳃病病原约氏黄杆菌减毒活疫苗及构建方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201510121169.7A CN104784684B (zh) | 2015-03-19 | 2015-03-19 | 一种鱼类烂鳃病病原约氏黄杆菌减毒活疫苗及构建方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN104784684A true CN104784684A (zh) | 2015-07-22 |
CN104784684B CN104784684B (zh) | 2017-12-12 |
Family
ID=53550169
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201510121169.7A Active CN104784684B (zh) | 2015-03-19 | 2015-03-19 | 一种鱼类烂鳃病病原约氏黄杆菌减毒活疫苗及构建方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN104784684B (zh) |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112322543A (zh) * | 2020-11-19 | 2021-02-05 | 肇庆大华农生物药品有限公司 | 一种柱状黄杆菌分离培养基 |
CN112516299A (zh) * | 2020-11-19 | 2021-03-19 | 肇庆大华农生物药品有限公司 | 一种柱状黄杆菌减毒灭活疫苗制备方法 |
-
2015
- 2015-03-19 CN CN201510121169.7A patent/CN104784684B/zh active Active
Non-Patent Citations (2)
Title |
---|
刘礼辉等: "TAIL-PCR方法克隆约氏黄杆菌aroA基因序列", 《安徽农业科学》 * |
胡钱东等: "约氏黄杆菌研究进展", 《生命科学研究》 * |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN112322543A (zh) * | 2020-11-19 | 2021-02-05 | 肇庆大华农生物药品有限公司 | 一种柱状黄杆菌分离培养基 |
CN112516299A (zh) * | 2020-11-19 | 2021-03-19 | 肇庆大华农生物药品有限公司 | 一种柱状黄杆菌减毒灭活疫苗制备方法 |
Also Published As
Publication number | Publication date |
---|---|
CN104784684B (zh) | 2017-12-12 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN108676756B (zh) | 贝莱斯芽孢杆菌及其作为水产病原菌抑制剂的应用 | |
Hamed et al. | Fish pathogen bacteria: Adhesion, parameters influencing virulence and interaction with host cells | |
CA2873305C (en) | Fish vaccine | |
CN105175518B (zh) | 凝结芽孢杆菌fm603产生的细菌素及其制备方法 | |
CN101342367B (zh) | 一种迟缓爱德华氏菌弱毒活疫苗 | |
CN104232535B (zh) | 鰤鱼诺卡氏菌诱变弱毒株及其应用 | |
CN102304484B (zh) | 金丽假交替单胞菌新菌株及其应用 | |
NO179360B (no) | Fremgangsmåte for fremstilling av en fiskevaksine samt en ikke-virulent, inntrengende mutant bakteriestamme inneholdt i vaksinen | |
CN109097298A (zh) | 一种富集培养法制备噬菌蛭弧菌制剂的方法 | |
CN113234647B (zh) | 一株大菱鲆来源的杀鲑气单胞菌及其应用 | |
CN109310721A (zh) | 噬菌体菌株及其应用 | |
CN104784684B (zh) | 一种鱼类烂鳃病病原约氏黄杆菌减毒活疫苗及构建方法 | |
CN106906141B (zh) | 一种嗜水气单胞菌活菌疫苗菌株的筛选方法 | |
CN107937301A (zh) | 一种解淀粉芽孢杆菌及其在水产养殖中的应用 | |
CN103272228B (zh) | 鲫鱼出血病多价载体疫苗及其应用 | |
CN106047783B (zh) | 杀香鱼假单胞菌ExoU基因敲除突变株及其应用 | |
CN102949713B (zh) | 一种新型枯草芽孢杆菌多价载体疫苗及其应用 | |
JP6709395B2 (ja) | 冷水病ワクチン及び冷水病の予防方法 | |
CN101024080A (zh) | 一种海豚链球菌白油佐剂灭活疫苗及其制备方法 | |
CN103509732B (zh) | 迟钝爱德华氏菌天然弱毒株及其应用 | |
CN104919036B (zh) | 迟钝爱德华氏菌突变株及其应用 | |
CN106417345B (zh) | 防治植物线虫的伴胞晶体蛋白组合物及其应用 | |
CN105031636A (zh) | 一种嗜水气单胞菌及维氏气单胞菌二联灭活疫苗及制备方法 | |
CN103966123B (zh) | 一株霍氏肠杆菌及其在制备抗菌药物中的应用 | |
CN103146666B (zh) | 猪链球菌胞壁水解酶、其编码基因及应用 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
EXSB | Decision made by sipo to initiate substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20190212 Address after: 443000 No. 519 Juxiang Road, Biological Industrial Park, Yichang High-tech Zone, Hubei Province Patentee after: Hubei Yishui Environmental Protection Technology Co.,Ltd. Address before: 513080 No. 1 Xingyu Road, Liwan District, Guangzhou City, Guangdong Province Patentee before: PEARL RIVER FISHERIES Research Institute CHINESE ACADEMY OF FISHERY SCIENCE |
|
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A live attenuated vaccine of Flavobacterium yoelii, the pathogen of fish gill rot, and its construction method Effective date of registration: 20220114 Granted publication date: 20171212 Pledgee: Yichang financing guarantee Group Co.,Ltd. Pledgor: Hubei Yishui Environmental Protection Technology Co.,Ltd. Registration number: Y2022980000496 |
|
PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20230214 Granted publication date: 20171212 Pledgee: Yichang financing guarantee Group Co.,Ltd. Pledgor: Hubei Yishui Environmental Protection Technology Co.,Ltd. Registration number: Y2022980000496 |
|
PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
CP03 | Change of name, title or address |
Address after: 443000 No. 519 Juxiang Road, Biological Industrial Park, Yichang High-tech Zone, Hubei Province Patentee after: Hubei Yishui Biotechnology Co.,Ltd. Country or region after: China Address before: 443000 No. 519 Juxiang Road, Biological Industrial Park, Yichang High-tech Zone, Hubei Province Patentee before: Hubei Yishui Environmental Protection Technology Co.,Ltd. Country or region before: China |
|
CP03 | Change of name, title or address |