CN104782671A - Bacteriostatic disinfecting disinfectant for laboratory department and preparation method for bacteriostatic disinfecting disinfectant - Google Patents
Bacteriostatic disinfecting disinfectant for laboratory department and preparation method for bacteriostatic disinfecting disinfectant Download PDFInfo
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Abstract
The invention discloses a bacteriostatic disinfecting disinfectant for the laboratory department. The bacteriostatic disinfecting disinfectant comprises the following components in percentage by weight: 0.2%-1.2% of glacial acetic acid, 1.0%-4.0% of hydrogen peroxide, 0.4%-0.6% of sodium lauryl sulfate, 0.5%-0.7% of an anionic surfactant, 0.3%-0.7% of chlorhexidine hydrochloride, 0.5 %-0.8% of a radix sophorae tonkinensis extract, 1.0%-3.0% of salicylic acid, 3.0%-5.0% of radix sophorae flavescentis, 4.0%-7.0% of Gleditsia sinensis Lam., and the balance of water. Tests prove that the urine analyzer cleaning disinfectant developed in the invention is colorless and transparent in appearance, stable in physico-chemical index performance, and has no crystallization, turbidity and precipitation during use.
Description
Technical field
The present invention relates to a kind of thimerosal, be specifically related to thimerosal of the antibacterial sterilization of clinical laboratory and preparation method thereof.
Background technology
Clinical laboratory is the bridge between clinical medicine and preclinical medicine, comprises the subdisciplines such as clinical chemistry, clinical microbiology, clinical immunology, hematology, osology and transfusion science.At present, inspection and the test of Klebsiella Pneumoniae (ATCC700603) and Neisseria gonorrhoeae (ATCC49226) is being carried out in control laboratory, because these two kinds of germs all have stronger contagiosity, if do not notice that the position such as hand easily via operator is transmitted, thus damage.
At present, the thimerosal being specifically designed to the antibacterial sterilization of clinical laboratory is seldom had in existing patent, publication number 101720760A(application number 200810157978.3) the multifunctional disinfectant that patent discloses a kind of antibacterial sterilization, its with glacial acetic acid and hydrogen peroxide for primary raw material, interpolation stabilizing agent salicylic acid, EDETATE DISODIUM, oxine mix, concrete composition percentage by weight is: glacial acetic acid 4.0%-6.0%, hydrogen peroxide 18.0%-22.0%, salicylic acid 0.3%-1.5%, EDETATE DISODIUM 0.3%-1.0%, oxine 0.3%-1.0%, surplus is water.Publication number 103766377A(application number 201210416140.8) patent discloses a kind of citric acid decalcification thimerosal being applied in dialysis machine and water treatment and preparation method thereof, this thimerosal comprises polyhexamethylene guanide, citric acid, lactic acid, malic acid, and surplus is deionized water.The component proportion of described thimerosal is polyhexamethylene guanide 0.001-5.0%, citric acid 10-50%, lactic acid 20.0%-40%, malic acid 10% ~ 30%, surplus are deionized water.This thimerosal is that primary raw material is formulated by citric acid, polyhexamethylene guanide, lactic acid and malic acid.Publication number 101664043(application number 200910307991.7) patent discloses the thimerosal in a kind of water route for antibacterial sterilization of sterilizing, clean and preparation method thereof, be made up of citric acid, acid regulator, acid protease, high purity water and hydrogen peroxide; Wherein acid regulator in sterilization, the mass concentration of cleaning in the water route thimerosal of antibacterial sterilization is 2%-5%, acid protease sterilization, the mass concentration of cleaning in the water route thimerosal of antibacterial sterilization be 3000-6000 SAPU/100ml, hydrogen peroxide is 8%-10% in sterilization, the mass concentration of cleaning in the water route thimerosal of antibacterial sterilization, citric acid is 40%-50% in sterilization, the mass concentration of cleaning in the water route thimerosal of antibacterial sterilization.
As can be seen here, thimerosal, in cleaning Medical Devices, has good bactericidal effect, but the prior art comprising above-mentioned patent document also comes with some shortcomings, first, existing correlation technique is also fewer, and the door do not had is for the thimerosal of the antibacterial sterilization of clinical laboratory.
Summary of the invention
The technical problem to be solved in the present invention is the cleaning and the thimerosal poor stability that overcome antibacterial sterilization in prior art, there is provided a kind of formula rationally, stable performance, easy to use, there is cleaning and sterilizing function, safety, nontoxic antibacterial sterilization cleaning and thimerosal.
The technical scheme that the present invention solves the problems of the technologies described above employing is:
The thimerosal of the antibacterial sterilization of clinical laboratory, in components by weight percent this product containing glacial acetic acid 0.2%-1.2%; Hydrogen peroxide 1.0%-4.0%; Sldium lauryl sulfate 0.4%-0.6%; Anion surfactant 0.5%-0.7%; Chlorhexidine hydrochloride 0.3%-0.7%; Sophorae subprostratae radix extract 0.5%-0.8%; Salicylic acid 1.0%-3.0%; Kuh-seng 3.0%-5.0%; Chinese honey locust 4.0%-7.0%; Surplus is water.
The thimerosal of the antibacterial sterilization of clinical laboratory, in components by weight percent this product containing glacial acetic acid 0.6%-0.8%; Hydrogen peroxide 2.0%-3.0%; Sldium lauryl sulfate 0.45%-0.55%; Anion surfactant 0.55%-0.65%; Chlorhexidine hydrochloride 0.4%-0.6%; Sophorae subprostratae radix extract 0.6%-0.7%; Salicylic acid 1.5%-2.5%; Kuh-seng 3.5%-4.5%; Chinese honey locust 5.0%-6.0%; Surplus is water.
The thimerosal of the antibacterial sterilization of clinical laboratory, in components by weight percent this product containing glacial acetic acid 0.7%; Hydrogen peroxide 2.5%; Sldium lauryl sulfate 0.5%; Anion surfactant 0.6%; Chlorhexidine hydrochloride 0.5%; Sophorae subprostratae radix extract 0.65%; Salicylic acid 2.0%; Kuh-seng 3.5%-4.5%; Chinese honey locust 5.5%; Surplus is water.
Another object of the present invention is to provide the method for the thimerosal preparing above-mentioned antibacterial sterilization.
(1) kuh-seng, Chinese honey locust are cleaned, chopping and sophorae subprostratae radix extract mix, and with 3-8 times amount 80% ethanol, with the speed diafiltration of 3ml/min, filtered by percolate, filtrate is stand-by;
(2) step (1) gained filtrate is placed in Agitation Tank and adds sldium lauryl sulfate, with dropper, salicylic acid is slowly instilled dispensing canister after being heated to 30-40 DEG C, add clear water after stopping heating, constantly stir 1-2 hour to obtain buffer solution, then will be placed in freezer 2-4 hour in buffer solution;
(3) in above-mentioned buffer solution, drip hydrogenperoxide steam generator, glacial acetic acid, anion surfactant and chlorhexidine hydrochloride simultaneously, continue stirring and obtain mixed liquor after 20-40 minute;
(4) 180-220 order screen pack is crossed to (3) gained mixed liquor, bottle and obtain thimerosal.
Foregoing preparation method, step (1) 4-7 times amount 80% ethanol, with the speed diafiltration of 3ml/min (preferably, with 5.5 times amount 80% ethanol, the speed diafiltration with 3ml/min).
Foregoing preparation method, slowly instills dispensing canister (preferred, with dropper, salicylic acid slowly to be instilled dispensing canister after being heated to 35 DEG C) with dropper by salicylic acid after step (2) is heated to 33-38 DEG C.
Foregoing preparation method, step (2) constantly stirs 1.3-1.8 hour to obtain buffer solution (preferred, constantly to stir 1.5 hours to obtain buffer solution).
Foregoing preparation method, step (2) will be placed in buffer solution freezer 2.5-3.5 hour (preferred, will to be placed in freezer 3 hours in buffer solution).
Foregoing preparation method, step (3) continuation is stirred after 25-35 minute and is obtained mixed liquor (preferred, to continue stirring and obtain mixed liquor after 30 minutes).
Foregoing preparation method, 190-210 order screen pack (preferred, 200 order screen packs crossed by mixed liquor) crossed by step (4) mixed liquor.
Effect of each component of thimerosal of the present invention is as follows:
Glacial acetic acid: acetic acid, being also acetic acid (36%--38%), glacial acetic acid (98%), chemical formula CH3COOH, is a kind of organic acid, is vinegar main component.Pure anhydrous acetic acid (glacial acetic acid) is colourless hygroscopic solid, and freezing point is 16.6 DEG C (62 ℉), and for clear crystal after solidifying, in faintly acid and erosion property is strong in its aqueous solution, steam is to eye and the irritant effect of nose.
Acetic acid can be used as acidity regulator, acidulant, pickling agent, flavoring agent, spices etc.It is also good antimicrobial, and this mainly can make pH be reduced to lower than the pH needed for microorganism the most suitable growth owing to it.Acetic acid is that China applies the earliest, uses maximum acids, is mainly used in compound seasoner, preparation wax, can, cheese, jelly etc.During for flavoring, after acetic acid can being diluted with water to 4% ~ 5% solution, adding in various flavoring and apply.Using vinegar as acid, be aided with the beverage that Pure natural nutraceutical makes and be called international style third generation beverage.
Hydrogen peroxide: hydrogen peroxide chemistry formula is H2O2, and pure hydrogen peroxide is nattier blue thick liquid, can mix with water by arbitrary proportion, and be a kind of strong oxidizer, the aqueous solution is commonly called as hydrogen peroxide, is colourless transparent liquid.Its aqueous solution is applicable to medical wound sterilization and environment disinfected and food sterilization.Can resolve into water and oxygen in the ordinary course of things, but decomposition rate is extremely slow, the way accelerating its reaction velocity adds catalyzer---manganese dioxide or irradiate with short-wave ray.The purposes of hydrogen peroxide divides medical, military and industrial three kinds, daily sterilization be medical hydrogen peroxide, medical hydrogen peroxide can kill pathogenic entero becteria, pyococcus, and pathogenic yeast bacterium, is generally used for surface disinfection.Hydrogen peroxide has oxidation, hydrogen peroxide bleaching mechanism but medical hydrogen peroxide concentration is equal to or less than 3%, wipes wound face, has burning sensation, surface is oxidized to white and emits bubble, just passable once by clean water, cross and just recover the original colour of skin in 3-5 minutes.Chemical industry is used as the raw material producing sodium perborate, SODIUM PERCARBONATE, glacial acetic acid, sodium chlorite, thiourea peroxide etc., the oxidant of tartaric acid, vitamin etc.Medical industry is used as bactericide, disinfectant, and produces the oxidant of tmtd insecticide and 40l antibacterial agent.Printing and dyeing industry is used as the bleaching agent of bafta, the color development after reducing dye dyeing.De-iron and other heavy metals is removed for the production of when metallic salt or other compounds.Also for electroplate liquid, can inorganic impurity be removed, improve plating quality.Also for the bleaching of wool, raw silk, ivory, paper pulp, fat etc.The hydrogen peroxide of high concentration can be used as rocket-powered fuel.Civilian: the peculiar smell of process drain of kitchen, to pharmacy buy hydrogen peroxide add water washing powder pour sewer into can decontamination, sterilization, sterilization;
Sldium lauryl sulfate: purposes: anion surfactant.Main be used as emulsifier, extinguishing chemical, blowing agent and textile auxiliary, electroplating additive, be also used as the blowing agent of toothpaste and paste, powdery, shampoo.
Anion surfactant: anion surfactant is divided into the large class of carboxylate, sulfuric acid, sulfonate and phosphate ester salt four, has good decontamination, foaming, dispersion, emulsification, the characteristic such as wetting.Be widely used as washing agent, foaming agent, wetting agent, emulsifier and dispersant.Output accounts for the first place of surfactant.Together can not use with cationic surfactant, generate precipitation in aqueous and lose effect.PAMA, because it has: 1, defecate and purify effect; 2, sedimentation facilitation; 3, facilitation is filtered; 4, thickening power and other effect.In liquid waste processing, sludge concentration and dewatering, ore dressing, coal washing, papermaking etc., fully can meet the requirement in various field.
Chlorhexidine hydrochloride: chlorhexidine hydrochloride is disinfection preservative, its restraining and sterilizing bacteria effect is extensively strong, stronger than the antibacterial effects of negative mattress to Gram-positive mattress, is strong than quaternary cationic surfactant, also effective to fungi, but invalid to acid fast bacteria, brood cell and virus.There being serum, blood is still effective when existing.To organizing nonirritant.
Sophorae subprostratae radix extract: subprostrate sophora, Chinese medicine name.For the dry root welding technology of leguminous plant sophora tonkinensis Gapnep.It is bitter, cold; Poisonous.Have clearing heat and detoxicating, effect of detumescence relieve sore throat, is usually used in fiery poison and pents up, acute tonsillitis larynx numbness, abscess of throat, gingivitis, aphthae.
Salicylic acid: 1, the standard of alkalimetry and iodometric titrationiodimetry titration.Fluorescence indicator, complexometric indicator.2, this product is used as antiscorching agent and produces ultra-violet absorber and blowing agent etc. in rubber industry.3, be used as to coordinate indicator, coordinate screening agent.The isoionic developer of titanium, zirconium, tungsten and preservative.4, salicylic acid is as additive in some faintly acid electrolyte, also can be used as the complexing agent of plating or chemical plating.5, cosmetics preservative.Be mainly used in the water class cosmetics such as floral water, miliaria lotion, quinine head water.Except antisepsis and sterilization effect, dispel the functions such as bad smell of perspiration, relieving itching and eliminating swelling, analgesic antiphlogistic in addition.6, mainly as the raw material of medical industry, for the preparation of medicines such as aspirin, sodium salicylate, salicylamide, ethoxybenzamide, phenyl salicytate, Niclosamides.Dye industry is for the preparation of chrome fast yellow rd, direct brown 3G N, acid chrome yellow etc.Also be used as vulcanization of rubber delayer and disinfection preservative etc.7, as the promoter of epoxy resin cure, also can be used as preservative.Can be used to prepare the synthetic perfume such as gaultherolin, salethyl.Dye industry is as the raw material preparing direct dyes and acid dye etc.Also can be used as rubber anti-chark agent, disinfectant etc.8, a small amount of for preparing the daily essences such as animal flavor essence.Trace is used for, in food, playing preservative.It is the important raw material of medical industry.
Kuh-seng: be the dry root of leguminous plant kuh-seng, spring, Qiu Erji excavate, removing root head and little supporting root, cleaning, drying, or take advantage of fresh slices, dry.It is bitter, cold.There is heat-clearing and damp-drying drug, desinsection, the merit of diuresis.For hot dysentery, have blood in stool, jaundice renal shutdown, leukorrhea with reddish discharge, swelling of vulva pruritus vulvae, eczema, wet sore, pruitus, mange leprosy, controls trichomonas vaginitis outward.
Chinese honey locust: the pod, seed, scolus etc. of Gleditsia officinalis all can be used as medicine, pod be used as medicine can eliminate the phlegm, diuresis; Seed is used as medicine and can controls tinea and defaecation is secret; Spina gleditsiae is used as medicine and can invigorates blood circulation and control sore tinea, and Gleditsia officinalis is used as medicine with fruit, seed.Chinese honey locust is containing triterpenoid saponin (gledinin, gleditschiasaponin), tannin, ceryl alcohol, nonacosane, stigmasterol, sitosterol etc.The function of have eliminating the phlegm, having one's ideas straightened out.Saponin(e element contained in Chinese honey locust is triterpene class and oligosaccharide, has anti-inflammatory, antiulcer, disease-resistant change effect, also has anticancer and improves the effects such as acquired immune deficiency syndrome (AIDS) immunity.
Compared with prior art the present invention prepares that disinfectant production cost is low, preparation technology is simple, process conditions are easy to realize, and product stability is good.
Embodiment
Describe technical scheme of the present invention in detail below in conjunction with embodiment and experimental example, but protection domain is not by this restriction.
embodiment 1the thimerosal of the antibacterial sterilization of clinical laboratory, in components by weight percent: glacial acetic acid 0.2%; Hydrogen peroxide 1.0%; Sldium lauryl sulfate 0.4%; Anion surfactant 0.5%; Chlorhexidine hydrochloride 0.3%; Sophorae subprostratae radix extract 0.5%; Salicylic acid 1.0%; Kuh-seng 3.0%; Chinese honey locust 4.0%; Surplus is water.
The preparation process of the thimerosal of the antibacterial sterilization of clinical laboratory is as follows:
(1) kuh-seng, Chinese honey locust are cleaned, chopping and sophorae subprostratae radix extract mix, and with 3 times amount 80% ethanol, with the speed diafiltration of 3ml/min, filtered by percolate, filtrate is stand-by;
(2) step (1) gained filtrate is placed in Agitation Tank and adds sldium lauryl sulfate, with dropper, salicylic acid is slowly instilled dispensing canister after being heated to 30 DEG C, add clear water after stopping heating, constantly stir 1-2 hour to obtain buffer solution, then will be placed in freezer in buffer solution 2 hours;
(3) in above-mentioned buffer solution, drip hydrogenperoxide steam generator, glacial acetic acid, anion surfactant and chlorhexidine hydrochloride simultaneously, continue stirring and obtain mixed liquor after 20 minutes;
(4) 180 order screen packs are crossed to (3) gained mixed liquor, bottle and obtain thimerosal.
stability test 1:get the thimerosal of above-described embodiment 1 gained for Urine Analyzer cleaning and sterilizing, carry out stability test, storage condition with reference to " disinfection technology standard " 2002 editions: at 37 DEG C, testing environment: temperature 35 DEG C, relative moisture 42%-48%.Result is as shown in table 1 below:
Table 1
Proved by 37 DEG C of accelerated tests, active ingredient glacial acetic acid and the salicylic content of the thimerosal for Urine Analyzer cleaning and sterilizing of embodiment 1 gained do not decline substantially, only have a small amount of salicylic acid to decompose; Show the having good stability of thimerosal of the present invention for Urine Analyzer cleaning and sterilizing, in storage and transportation, a large amount of decomposition can not occur.
embodiment 2the thimerosal of the antibacterial sterilization of clinical laboratory, in components by weight percent: glacial acetic acid 1.2%; Hydrogen peroxide 4.0%; Sldium lauryl sulfate 0.6%; Anion surfactant 0.7%; Chlorhexidine hydrochloride 0.7%; Sophorae subprostratae radix extract 0.8%; Salicylic acid 3.0%; Kuh-seng 5.0%; Chinese honey locust 7.0%; Surplus is water.
The preparation process of the thimerosal of the antibacterial sterilization of clinical laboratory is as follows:
(1) kuh-seng, Chinese honey locust are cleaned, chopping and sophorae subprostratae radix extract mix, and with 8 times amount 80% ethanol, with the speed diafiltration of 3ml/min, filtered by percolate, filtrate is stand-by;
(2) step (1) gained filtrate is placed in Agitation Tank and adds sldium lauryl sulfate, with dropper, salicylic acid is slowly instilled dispensing canister after being heated to 40 DEG C, add clear water after stopping heating, constantly stir 2 hours to obtain buffer solution, then will be placed in freezer in buffer solution 4 hours;
(3) in above-mentioned buffer solution, drip hydrogenperoxide steam generator, glacial acetic acid, anion surfactant and chlorhexidine hydrochloride simultaneously, continue stirring and obtain mixed liquor after 40 minutes;
(4) 220 order screen packs are crossed to (3) gained mixed liquor, bottle and obtain thimerosal.
stability test 2:get the thimerosal of above-described embodiment 1 gained for Urine Analyzer cleaning and sterilizing, carry out stability test, storage condition with reference to " disinfection technology standard " 2002 editions: 37 DEG C of placements.Testing environment: temperature 35 DEG C, relative moisture 42%-48%.Result is as shown in table 2 below:
Table 2
Proved by 37 DEG C of accelerated tests, active ingredient glacial acetic acid and the salicylic content of the thimerosal for Urine Analyzer cleaning and sterilizing of embodiment 2 gained do not decline substantially, only have a small amount of salicylic acid to decompose; Show the having good stability of thimerosal of the present invention for Urine Analyzer cleaning and sterilizing, in storage and transportation, a large amount of decomposition can not occur.
embodiment 3the thimerosal of the antibacterial sterilization of clinical laboratory, in components by weight percent: glacial acetic acid 0.6%; Hydrogen peroxide 2.0%; Sldium lauryl sulfate 0.45%; Anion surfactant 0.55%; Chlorhexidine hydrochloride 0.4%; Sophorae subprostratae radix extract 0.6%; Salicylic acid 1.5%; Kuh-seng 3.5%; Chinese honey locust 5.0%; ; Surplus is water.
The preparation process of the thimerosal of the antibacterial sterilization of clinical laboratory is as follows:
(1) kuh-seng, Chinese honey locust are cleaned, chopping and sophorae subprostratae radix extract mix, and with 4 times amount 80% ethanol, with the speed diafiltration of 3ml/min, filtered by percolate, filtrate is stand-by;
(2) step (1) gained filtrate is placed in Agitation Tank and adds sldium lauryl sulfate, with dropper, salicylic acid is slowly instilled dispensing canister after being heated to 33 DEG C, add clear water after stopping heating, constantly stir 1-2 hour to obtain buffer solution, then will be placed in freezer in buffer solution 2.5 hours;
(3) in above-mentioned buffer solution, drip hydrogenperoxide steam generator, glacial acetic acid, anion surfactant and chlorhexidine hydrochloride simultaneously, continue stirring and obtain mixed liquor after 25 minutes;
(4) 190 order screen packs are crossed to (3) gained mixed liquor, bottle and obtain thimerosal.
stability test 3:get the thimerosal for Urine Analyzer cleaning and sterilizing prepared by above-described embodiment 3, carry out stability test, storage condition with reference to " disinfection technology standard " 2002 editions: place at 37 DEG C.Testing environment: temperature 35 DEG C, relative moisture 42%-48%.Result is as shown in table 3 below:
Table 3
Proved by 37 DEG C of accelerated tests, active ingredient glacial acetic acid and the salicylic content of the thimerosal for Urine Analyzer cleaning and sterilizing of embodiment 3 gained do not decline substantially, only have a small amount of salicylic acid to decompose; Show the having good stability of thimerosal of the present invention for Urine Analyzer cleaning and sterilizing, in storage and transportation, a large amount of decomposition can not occur.
embodiment 4the thimerosal of the antibacterial sterilization of clinical laboratory, in components by weight percent: glacial acetic acid 0.8%; Hydrogen peroxide 3.0%; Sldium lauryl sulfate 0.55%; Anion surfactant 0.65%; Chlorhexidine hydrochloride 0.6%; Sophorae subprostratae radix extract 0.7%; Salicylic acid 2.5%; Kuh-seng 4.5%; Chinese honey locust 6.0%; Surplus is water.
The preparation process of the thimerosal of the antibacterial sterilization of clinical laboratory is as follows:
(1) kuh-seng, Chinese honey locust are cleaned, chopping and sophorae subprostratae radix extract mix, and with 7 times amount 80% ethanol, with the speed diafiltration of 3ml/min, filtered by percolate, filtrate is stand-by;
(2) step (1) gained filtrate is placed in Agitation Tank and adds sldium lauryl sulfate, with dropper, salicylic acid is slowly instilled dispensing canister after being heated to 38 DEG C, add clear water after stopping heating, constantly stir 1.8 hours to obtain buffer solution, then will be placed in freezer in buffer solution 3.5 hours;
(3) in above-mentioned buffer solution, drip hydrogenperoxide steam generator, glacial acetic acid, anion surfactant and chlorhexidine hydrochloride simultaneously, continue stirring and obtain mixed liquor after 35 minutes;
(4) 210 order screen packs are crossed to (3) gained mixed liquor, bottle and obtain thimerosal.
embodiment 5the thimerosal of the antibacterial sterilization of clinical laboratory, in components by weight percent, glacial acetic acid 0.7%; Hydrogen peroxide 2.5%; Sldium lauryl sulfate 0.5%; Anion surfactant 0.6%; Chlorhexidine hydrochloride 0.5%; Sophorae subprostratae radix extract 0.65%; Salicylic acid 2.0%; Kuh-seng 3.5%-4.5%; Chinese honey locust 5.5%; Surplus is water.
The preparation process of the thimerosal of the antibacterial sterilization of clinical laboratory is as follows:
(1) kuh-seng, Chinese honey locust are cleaned, chopping and sophorae subprostratae radix extract mix, and with 5.5 times amount 80% ethanol, with the speed diafiltration of 3ml/min, filtered by percolate, filtrate is stand-by;
(2) step (1) gained filtrate is placed in Agitation Tank and adds sldium lauryl sulfate, with dropper, salicylic acid is slowly instilled dispensing canister after being heated to 35 DEG C, add clear water after stopping heating, constantly stir 1.5 hours to obtain buffer solution, then will be placed in freezer in buffer solution 3 hours;
(3) in above-mentioned buffer solution, drip hydrogenperoxide steam generator, glacial acetic acid, anion surfactant and chlorhexidine hydrochloride simultaneously, continue stirring and obtain mixed liquor after 30 minutes;
(4) 200 order screen packs are crossed to (3) gained mixed liquor, bottle and obtain thimerosal.
experimental example 1:to embodiment 5 prepare thimerosal with shigella dysenteriae, mould, staphylococcus and the salmonella sterilization Simulation field test to medicine equipment:
Get shigella dysenteriae suspension, mould bacteria suspension, staphylococcus suspension and salmonella suspension respectively to drip and contaminate in the coating of polyfluortetraethylene pipe inside evenly, be placed in 37 DEG C of constant incubators dry, prepare bacterial carrier.With the product of gained of the present invention for disinfectant, carry out cleaning and sterilizing according to Urine Analyzer machine operation instructions sterilization code, result is as shown in table 4 below:
This thimerosal of table 4 is to the kill oncomelania of bacterium
Result shows the kill oncomelania >5.89 of this product to the shigella dysenteriae contaminated on polyfluortetraethylene pipe, mould, staphylococcus and salmonella, shows that this product has good disinfection effect.
experimental example 2:in order to verify the disinfection effect of embodiment 5 gained thimerosal, get shigella dysenteriae suspension, mould bacteria suspension, staphylococcus suspension and salmonella suspension respectively to drip and contaminate in the coating of polyfluortetraethylene pipe inside evenly, be placed in 37 DEG C of constant incubators dry, prepare bacterial carrier.By the bacterial carrier prepared, be divided into two groups, experimental group and control group, experimental group with the product of gained of the present invention for disinfectant, cleaning and sterilizing is carried out according to Urine Analyzer machine operation instructions sterilization code, control group carries out cleaning and sterilization (the such as routine disinfection agent such as citric acid disinfectant, the low bubble cleaning agent of medical alkalescence) with other disinfectants to Urine Analyzer machine, and result is as shown in table 5 below:
Table 5 is for gained thimerosal of the present invention is to the kill oncomelania of bacterium
Result: two groups of sterilization comparitive study, this product is to the kill oncomelania >5.89 of the shigella dysenteriae contaminated on polyfluortetraethylene pipe, mould, staphylococcus and salmonella, obvious bactericidal effect is better than control group, shows that this product has good disinfection effect.Prove through overtesting, the Urine Analyzer of the present invention's development cleans and thimerosal appearance colorless is transparent, physical and chemical index stable performance, has no crystallization, muddiness, precipitation during use, and every physical and chemical index performance is stable.
With reference to embodiments of the invention, explanation is given to the present invention above.But these embodiments are only for illustrative purposes, and are not intended to limit the scope of the invention.Scope of the present invention is by claims and equivalents thereof.Do not depart from the scope of the present invention, those skilled in the art can make a variety of substitutions and modifications, and these substitutions and modifications all should fall within the scope of the present invention.
Claims (10)
1. the thimerosal of the antibacterial sterilization of clinical laboratory, is characterized in that: in components by weight percent this product containing glacial acetic acid 0.2%-1.2%; Hydrogen peroxide 1.0%-4.0%; Sldium lauryl sulfate 0.4%-0.6%; Anion surfactant 0.5%-0.7%; Chlorhexidine hydrochloride 0.3%-0.7%; Sophorae subprostratae radix extract 0.5%-0.8%; Salicylic acid 1.0%-3.0%; Kuh-seng 3.0%-5.0%; Chinese honey locust 4.0%-7.0%; Surplus is water.
2. the thimerosal of the antibacterial sterilization of clinical laboratory according to claim 1, is characterized in that: in components by weight percent this product containing glacial acetic acid 0.6%-0.8%; Hydrogen peroxide 2.0%-3.0%; Sldium lauryl sulfate 0.45%-0.55%; Anion surfactant 0.55%-0.65%; Chlorhexidine hydrochloride 0.4%-0.6%; Sophorae subprostratae radix extract 0.6%-0.7%; Salicylic acid 1.5%-2.5%; Kuh-seng 3.5%-4.5%; Chinese honey locust 5.0%-6.0%; Surplus is water.
3. the thimerosal of the antibacterial sterilization of clinical laboratory according to claim 1, is characterized in that: in components by weight percent this product containing glacial acetic acid 0.7%; Hydrogen peroxide 2.5%; Sldium lauryl sulfate 0.5%; Anion surfactant 0.6%; Chlorhexidine hydrochloride 0.5%; Sophorae subprostratae radix extract 0.65%; Salicylic acid 2.0%; Kuh-seng 3.5%-4.5%; Chinese honey locust 5.5%; Surplus is water.
4. the thimerosal of the antibacterial sterilization of the clinical laboratory according to any one of claim 1-3, is characterized in that: step is as follows:
(1) kuh-seng, Chinese honey locust are cleaned, chopping and sophorae subprostratae radix extract mix, and with 3-8 times amount 80% ethanol, with the speed diafiltration of 3ml/min, filtered by percolate, filtrate is stand-by;
(2) step (1) gained filtrate is placed in Agitation Tank and adds sldium lauryl sulfate, with dropper, salicylic acid is slowly instilled dispensing canister after being heated to 30-40 DEG C, add clear water after stopping heating, constantly stir 1-2 hour to obtain buffer solution, then will be placed in freezer 2-4 hour in buffer solution;
(3) in above-mentioned buffer solution, drip hydrogenperoxide steam generator, glacial acetic acid, anion surfactant and chlorhexidine hydrochloride simultaneously, continue stirring and obtain mixed liquor after 20-40 minute;
(4) 180-220 order screen pack is crossed to (3) gained mixed liquor, bottle and obtain thimerosal.
5. preparation method according to claim 4, is characterized in that: step (1) 4-7 times amount 80% ethanol, with the speed diafiltration of 3ml/min (preferably, with 5.5 times amount 80% ethanol, the speed diafiltration with 3ml/min).
6. preparation method according to claim 4, is characterized in that: with dropper, salicylic acid is slowly instilled dispensing canister (preferred, with dropper, salicylic acid slowly to be instilled dispensing canister after being heated to 35 DEG C) after step (2) is heated to 33-38 DEG C.
7. preparation method according to claim 4, is characterized in that: step (2) constantly stirs 1.3-1.8 hour to obtain buffer solution (preferred, constantly to stir 1.5 hours to obtain buffer solution).
8. preparation method according to claim 4, is characterized in that: step (2) will be placed in buffer solution freezer 2.5-3.5 hour (preferred, will to be placed in freezer 3 hours in buffer solution).
9. preparation method according to claim 4, is characterized in that: step (3) continuation is stirred after 25-35 minute and obtained mixed liquor (preferred, to continue stirring and obtain mixed liquor after 30 minutes).
10. preparation method according to claim 4, is characterized in that: 190-210 order screen pack (preferred, 200 order screen packs crossed by mixed liquor) crossed by step (4) mixed liquor.
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