CN104777108B - The detection means and method of a kind of chlorophyll content - Google Patents

The detection means and method of a kind of chlorophyll content Download PDF

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CN104777108B
CN104777108B CN201510059807.7A CN201510059807A CN104777108B CN 104777108 B CN104777108 B CN 104777108B CN 201510059807 A CN201510059807 A CN 201510059807A CN 104777108 B CN104777108 B CN 104777108B
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chlorophyll
sample
absorbance
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tested
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CN104777108A (en
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李民赞
糜修尘
郑立华
张瑶
张琴
孙红
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China Agricultural University
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China Agricultural University
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Abstract

The invention discloses a kind of detection means of chlorophyll content and method, this method includes:Using the chlorophyll solution of various concentrations, chlorophyll detection model is established;Sample to be tested is detected using the incident light of two wavelength, obtains the absorbance of the chlorophyll of the sample to be tested;The absorbance of the chlorophyll of the sample to be tested is substituted into the chlorophyll detection model, obtains the concentration of the chlorophyll of the sample to be tested.This method is respectively 645nm with wavelength and 663nm light irradiation sample, the absorbance under corresponding wavelength can be obtained by the intensity of photoelectric sensor measurement transmitted light, so as to calculate the relative concentration values of chlorophyll.Major advantage is:Analyze speed is fast, measurement range is wide, preparation of samples is simple, does not consume sample, without chemical contamination etc..

Description

The detection means and method of a kind of chlorophyll content
Technical field
The present invention relates to technical field of spectral detection, and in particular to the detection means and method of a kind of chlorophyll content.
Background technology
It as the saying goes:" breed fish and first support water ".Want to obtain aquaculture success, it is crucial that regulating and controlling water quality is handled properly. And the key of regulating and controlling water quality is in aquaculture:Dissolved oxygen, transparency, acid-base value etc..Wherein dissolved oxygen amount is aquiculture animal Biological element, chlorophyll content decides the dissolved oxygen amount in water body, and chlorophyll is the important composition composition of algae, chlorophyll Content can also reflect water quality condition to a certain extent.
The method that water body chlorophyll content is measured with spectrophotometer 721 belongs to sampling analysis mensuration, common practice It is that scene is taken and laboratory is taken back after water sample analyze measure, it is necessary to through several steps such as over-sampling, filtering, extraction, measure. Such assay method accuracy of detection is higher, and can analyze constituent content.But the preprocessing process of sample is complicated, easily exists Secondary pollution is brought in operating process;Sample takes back laboratory and needs the regular hour from marine or lake, and chlorophyll exists In the presence of microorganism, it may be decomposed, it is difficult to accurately reflect the truth in ecosystem Determination of Chlorophyll.
Chlorophyll monitoring system using the type chlorophyll sensors of YSI 6025 as core.The type chlorophyll sensors of YSI 6025 Using living body measurement method, that is, the feature that make use of chlorophyll fluorescence to respond, fluorescence is discharged under specific wavelength light photograph, it is glimmering Luminous intensity is proportional to chlorophyll concentration.The fluorescence intensity caused by exciting is very faint, therefore the detection method is in actual inspection It is very high to environmental requirement in survey, easily disturbed by ambient light.Simultaneously because the uncertainty of water sample impurity, causes to examine Surveying result also can be by a certain degree of interference.
The content of the invention
For in the prior art the defects of, the invention provides a kind of detection means of chlorophyll content and method, the party Method analyze speed is fast, measurement range is wide.
In a first aspect, the present invention provides a kind of detection method of chlorophyll content, including:
Using the chlorophyll solution of various concentrations, chlorophyll detection model is established;
Sample to be tested is detected using the incident light of two wavelength, obtains the extinction of the chlorophyll of the sample to be tested Degree;
The absorbance of the chlorophyll of the sample to be tested is substituted into the chlorophyll detection model, obtains the sample to be tested Chlorophyll concentration.
Optionally, the chlorophyll solution using various concentrations, chlorophyll detection model is established, including:
The standard chlorophyll solution of various concentrations is detected using the light under two wavelength;
Absorbance and chlorophyll concentration corresponding to the light of described two wavelength are calculated respectively;
According to the absorbance and the chlorophyll concentration, chlorophyll detection model is established.
Optionally, described two wavelength are 663nm and 645nm.
Second aspect, present invention also offers a kind of detection means of chlorophyll content, including:Model building module, light Electric detecting module and detection module, the model building module are arranged in the detection module, the photoelectric detection module with The detection module is connected;
The model building module, for the chlorophyll solution using various concentrations, establish chlorophyll detection model;
The photoelectric detection module, for being detected using the incident light of two wavelength to sample to be tested, described in acquisition The absorbance of the chlorophyll of sample to be tested;
The detection module, mould is detected for the absorbance of the chlorophyll of the sample to be tested to be substituted into the chlorophyll Type, obtain the concentration of the chlorophyll of the sample to be tested.
Optionally, the model building module, is specifically included:
The standard chlorophyll solution of various concentrations is detected using the light under two wavelength;
Absorbance and chlorophyll concentration corresponding to the light of described two wavelength are calculated respectively;
According to the absorbance and the chlorophyll concentration, chlorophyll detection model is established.
Optionally, described two wavelength are 663nm and 645nm.
Optionally, the detection module includes:Voltage conversion circuit, light source current stabilization circuit, I-U change-over circuits, filtering are put Big circuit, A/D change-over circuits, display circuit and storage circuit.
Optionally, the voltage conversion circuit, for being powered by voltage conversion to the detection module.
Optionally, the light source current stabilization circuit, for light source power supply.
Optionally, the storage circuit, the absorbance of the chlorophyll for storing the sample to be tested.
As shown from the above technical solution, the detection means and method of a kind of chlorophyll content provided by the invention, this method With wavelength it is respectively 645nm and 663nm light irradiation sample, the intensity of transmitted light is measured by photoelectric sensor can obtain pair The absorbance under wavelength is answered, so as to calculate the relative concentration values of chlorophyll.Major advantage is:Analyze speed is fast, measurement model Enclose that wide, preparation of samples is simple, does not consume sample, without chemical contamination etc..
Brief description of the drawings
Fig. 1 is a kind of schematic flow sheet of the detection method for chlorophyll content that one embodiment of the invention provides;
Fig. 2 is the structure of the detecting device schematic diagram that one embodiment of the invention provides;
Fig. 3 is the light channel structure design diagram that another embodiment of the present invention provides;
Fig. 4 is the voltage conversion circuit schematic diagram that one embodiment of the invention provides;
Fig. 5 is the light source current stabilization power supply circuit schematic diagram that one embodiment of the invention provides;
Fig. 6 is the I-U change-over circuit schematic diagrames that one embodiment of the invention provides;
Fig. 7 is the filter amplification circuit schematic diagram that one embodiment of the invention provides;
Fig. 8 is the A/D change-over circuit schematic diagrames that one embodiment of the invention provides;
Fig. 9 is the display circuit schematic diagram that one embodiment of the invention provides;
Figure 10 is the serial port circuit and synchronous USB flash disk memory module schematic diagram that one embodiment of the invention provides;
Figure 11 is the Electric signal processing process schematic that one embodiment of the invention provides;
Figure 12 is the schematic flow sheet of one embodiment of the invention detection means detection;
Figure 13 is the measurement procedure schematic diagram that one embodiment of the invention provides;
Figure 14 is the USB flash disk Stored Procedure schematic diagram that one embodiment of the invention provides;
Embodiment
Below in conjunction with the accompanying drawings, the embodiment of invention is further described.Following examples are only used for more clear Illustrate to Chu technical scheme, and can not be limited the scope of the invention with this.
Fig. 1 shows a kind of schematic flow sheet of the detection method of chlorophyll content provided in an embodiment of the present invention, such as Fig. 1 Shown, this method comprises the following steps:
101st, using the chlorophyll solution of various concentrations, chlorophyll detection model is established;
102nd, sample to be tested is detected using the incident light of two wavelength, obtains the chlorophyll of the sample to be tested Absorbance;Described two wavelength are 663nm and 645nm.
103rd, the absorbance of the chlorophyll of the sample to be tested is substituted into the chlorophyll detection model, obtained described to be measured The concentration of the chlorophyll of sample.
The above method is respectively 645nm with wavelength and 663nm light irradiation sample, and transmitted light is measured by photoelectric sensor Intensity can obtain absorbance under corresponding wavelength, so as to calculate the relative concentration values of chlorophyll.Major advantage is:Point Analysis speed is fast, measurement range is wide, preparation of samples is simple, does not consume sample, without chemical contamination etc..
The chlorophyll solution of various concentrations is utilized described in above-mentioned steps 101, establishes chlorophyll detection model, including:
1011st, the standard chlorophyll solution of various concentrations is detected using the light under two wavelength;
1012nd, absorbance and chlorophyll concentration corresponding to the light of described two wavelength are calculated respectively;
1013rd, according to the absorbance and the chlorophyll concentration, chlorophyll detection model is established.
The above method has the following advantages that:
(1) transmission principle is used, improves determination efficiency
Traditional chlorophyll detection method generally requires to carry out complicated sample preprocessing, and being carried plus instrument is inconvenient to lead Cause sample to pass through a very long time from measurement is collected, cause the decomposition of chlorophyll, have impact on measurement accuracy.Nothing of the present invention Sample preprocessing is needed, can now gather existing measurement.The integrality of sample is ensure that, improves measurement accuracy, and safely without dirt Dye.
Chlorophyll content detection is carried out using transmission beam method principle, can avoid what is be likely to occur in sampling, preprocessing process Phenomena such as chlorophyll degradation, other Substances Pollutions, so as to ensure that the accuracy of chlorophyll concentration detection.Due to fluorescent intensity compared with It is weak, easily disturbed by ambient light when detecting, therefore the optical texture of instrument can be simplified using transmission beam method Cleaning Principle, together When lowering apparatus environmental requirement when in use, so as to exploitation, making and the use cost of lowering apparatus, popularization to instrument and Using advantageously.
(2) reliable high-precision water body chlorophyll content detection model is provided
For Accurate Prediction water body chlorophyll content, the present invention directly establishes and using the chlorophyll in spectrophotometric principle Assay model.First with the standard chlorophyll solution of various concentrations, standard chlorophyll detection model is established.Utilize two Light under wavelength detects to the standard chlorophyll solution of various concentrations, calculates corresponding absorbance.Survey is utilized afterwards The absorbance and corresponding chlorophyll concentration obtained, the detection model (R2=0.84) of high-precision chlorophyll concentration is established, and will The detection model is directly embedded into instrument.Model is demarcated and corrected first with chlorophyll titer during measurement, so Uniform Sample is measured using the incident light of two different wave lengths afterwards, two are measured respectively using optical texture and independent circuits The transmissivity of light under individual wavelength, chlorophyll content is finally calculated according to embedded water body chlorophyll concentration detection model.
(3) accuracy of detection is ensured from structure
In order to improve the accuracy of detector, influence of the particle movement to result in water is reduced, is distinguished using upper and lower two-way The mode of collection measures chlorophyll content, and last chlorophyll content is drawn by averaging.This measuring method is by water sample Influence of the particulate deposits to chlorophyll content is preferably minimized in microorganism uneven distribution and measurement process, improves measurement essence Degree.
(4) data provided convenience support instrument
The present invention uses for measuring in real time, in order to preserve historical record and increase amount of storage, there is provided USB flash disk is synchronous Store function, the needs repeatedly measured are met, facilitate the realization of the calling of data and water quality distribution map in the future.
Detection water body chlorophyll content is to preferably instruct aquaculture personnel to regulate and control water quality, improves production Amount.
Fig. 2 shows a kind of detection means of chlorophyll content provided in an embodiment of the present invention, including:Model establishes mould Block, photoelectric detection module and detection module, the model building module are arranged in the detection module, the photodetection mould Block is connected with the detection module;
The model building module, for the chlorophyll solution using various concentrations, establish chlorophyll detection model;
The photoelectric detection module, for being detected using the incident light of two wavelength to sample to be tested, described in acquisition The absorbance of the chlorophyll of sample to be tested;Described two wavelength are 663nm and 645nm.
The detection module, mould is detected for the absorbance of the chlorophyll of the sample to be tested to be substituted into the chlorophyll Type, obtain the concentration of the chlorophyll of the sample to be tested.
The model building module, is specifically included:
The standard chlorophyll solution of various concentrations is detected using the light under two wavelength;
Absorbance and chlorophyll concentration corresponding to the light of described two wavelength are calculated respectively;
According to the absorbance and the chlorophyll concentration, chlorophyll detection model is established.
According to Lambert-Beer laws, under certain condition the absorbance D of certain colored solutions and wherein solute concentration C and Liquid layer thickness L is directly proportional, i.e.,:In D=aCL formulas, a is proportionality constant.If containing several extinction materials in solution, this mixing Absorbance of the liquid under a certain wavelength be equal to each component absorbance under respective wavelength summation, here it is absorbance plus and Property.If determining chlorophyll content, the absorbance A of the water sample at a particular wavelength only need to be determined, and according to chlorophyll a, b in the ripple Specific absorptivity under long can obtain its concentration.It is used when determining chlorophyll a, b in order to exclude the interference of carotenoid Maximum absorption band of the monochromatic wavelength selection chlorophyll at feux rouges.Maximum absorption band of the known chlorophyll a, b in red light district Respectively 663nm and 645nm, it is 663nm and 645nm because choosing sensitive band.
It is A=lg [I for the absorbance calculation formula under two wave bandss(λ)/Iz(λ)], wherein A is absorbance, Is(λ) For the transmission value of titer (distilled water), Iz(λ) is the transmission of water sample.Maximum absorption band of the known chlorophyll a, b in red light district Respectively 663nm and 645nm, know again under wavelength 663nm, specific absorptivity of the chlorophyll a, b in the solution is respectively 82.04 and 9.27, it is respectively 16.75 and 45.6 under wavelength 645nm, relationship below is listed according to additive property principle:
A663=82.04Ca+9.27Cb A645=16.75Ca+45.6Cb
A663 and A645 is respectively absorbance of the chlorophyll solution in wavelength 663nm and 645nm in formula, and Ca, Cb distinguish For the concentration of chlorophyll a, b, unit mg/L.Solving equations can obtain:
Ca=12.72A663-2.59A645 Cb=22.88A645-4.67A663
Ca, Cb are respectively the concentration of chlorophyll a, b in formula, unit mg/L.Ca is added with Cb to produce chlorophyll always dense Spend (CT, mg/L):CT=Ca+Cb=20.29A645+8.05A663
It will be located in model insertion single-chip microcomputer in detection means, for being directly invoked during measurement.
Detection means is made up of opticator and control section.Opticator includes 4 LED light sources transmitting of 2 wave bands Device, it is powered by monolithic processor controlled light source driving circuit, is impinged perpendicularly on after collimation lens is collimated in water, Gathered accordingly by 4 photodetectors;Control section includes amplifying circuit, filter circuit, A/D change-over circuits, monolithic Machine control circuit, liquid crystal display circuit and synchronous USB flash disk storage circuit.Detector is lighted successively using single-chip microcomputer control light source, The single band optical signal that light source is sent is impinged perpendicularly in sample after collimation lens to be measured, by the absorption of sample, Scattering and transmission, are acquired, optical signal is converted into electric signal by photodetector by corresponding photodetector.Visited through photoelectricity The electric signal that device is converted into is surveyed to be kept in by 89S52 single-chip microcomputers after the processing such as amplification, filtering, A/D conversions, and with receiving successively The optical signal come in calculates to be substituted into water body chlorophyll content detection model after absorbance and is calculated together, the result after calculating Exported by 128*64 liquid crystal displays, while the functions such as USB flash disk storage can be realized.Fig. 2 is system global structure design drawing.
The detection module includes:Voltage conversion circuit, light source current stabilization circuit, I-U change-over circuits, filter amplification circuit, A/D change-over circuits, display circuit and storage circuit.
Sensor main will be responsible for the driving of light source, the collection of optical signal, the processing of photoelectric conversion and electric signal, amplification, The work such as collection, transmission.Fig. 2 is the general structure block diagram of sensor.Below will respectively from mechanical structure, hardware circuit design, Software circuit design etc. describes in detail.
A, sensor mechanical structure designs
A, Fundamentals of Sensors design
Chlorophyll is one of important composition composition of algae, is the important factor for carrying out algae light and effect.Chlorophyll contains The height of amount is relevant with quality of water environment, directly determines the oxygen content height of water body.Chlorophyll has special optical characteristics. Most of chlorophyll a and whole chlorophyll bs have the function that to absorb luminous energy, but the leaf of only only a few special state is green Plain a molecules, just have the function that to convert light energy into electric energy.Therefore we only need according to absorption of the chlorophyll to visible spectrum Absorbance A of the sample under two specific wavelengths is determined using survey meter, and according to chlorophyll a, b and carotenoid at this Specific absorptivity under wavelength can obtain its concentration.When determining chlorophyll a, b in order to exclude the interference of carotenoid, institute With maximum absorption band of the monochromatic wavelength selection chlorophyll at feux rouges.It is added i.e. after obtaining the concentration of chlorophyll a, b respectively Obtain chlorophyll total concentration.
B, light path system designs
Light path system mainly completes the transmission of optical signal by collimation lens, and optical signal is completed by photodetector Receive, be mainly made up of two parts, optical signal transmission path and receiving portion.
For the accuracy of detection, it is necessary to select suitable light source and its acquisition mode.According to low-power consumption, narrow bandwidth, just The principle of formula and the principle of detector are taken, selects the light emitting diode of 2 different-wavebands as incident light source, collimation lens conduct Transmission channel.Light path design after the collimated lens of light emitting diode as shown in figure 3, impinge perpendicularly in the water sample of sample bottle, warp Gathered after crossing the absorption, scattering and transmission of water sample by corresponding sensor to complete transformation of the optical signal to electric signal.This detection Instrument the upper and lower are same structure.
Single-chip microcomputer controls lightening LED lamp 1,2,3,4 successively, respectively by the acquisition of transmission signal of detector 1,2,3,4, adopts every time Corresponding lamp lights next lamp again after going out after having collected.
C, sensor hardware circuit design
Instrument is powered by a 9V battery after power circuit is changed to each circuit, and four LED light sources are by constant-current source list Solely power supply.Optical signal is changed into after electric signal, it is necessary to which the progress Electric signal processing work of a series of hardware circuit, mainly includes I-U change-over circuits, filter amplification circuit, A/D change-over circuits, liquid crystal display circuit and USB flash disk storage circuit.
(1) voltage conversion circuit and light source current stabilization circuit
Power supply used in portable detector is 9 volts of dry cells, and the operational voltage value of each chip is 5 volts in circuit, this Power-switching circuit is needed to realize.L7805 chips are used to be used for the 9V photovoltaic conversions of battery as 5V chip power supply voltages, figure 4 be voltage conversion circuit figure.The voltage conversion circuit, for being powered by voltage conversion to the detection module.
Light source intensity and spectral ripple can be caused because extraneous power supply is unstable, it should to LED light source using constant-current source electricity Road powers.Such as Fig. 5, constant current-supplying circuit is formed with LM317.Wherein LM317 input end groundings, according to output end and control terminal it Between formed 1.25V burning voltages principle, form current stabilization source circuit and provide constant-current source for LED.The light source current stabilization circuit, is used for To light source power supply.
(2) I-U change-over circuits
Signal is sent by light emitting diode used by due to the system, is transmitted by collimation lens, and signal is fainter, The electric signal obtained again after the conversion of InGaAs photodetectors is fainter, in addition, being also mingled with this process each The interference of kind noise, therefore it is the I- realized by CA3140 to have selected the integrated operational amplifier CA3140, Fig. 6 of high input impedance U change-over circuits, while realizing I-U conversions, realize an enlarging function of small-signal.
(3) filter amplification circuit
The secondary enlarging function of small-signal is realized by LM358, is put signal by the resistance for adjusting R9 potentiometers It is big 380 times, signal is sent into by AD7705 by the low-pass first order filter of R-C compositions and carries out A/D conversions.
(4) A/D change-over circuits
The analog signal amplified twice is changed into data signal by Fig. 8 A/D change-over circuits, is sent into 89S52 single-chip microcomputer System is handled, and A/D change-over circuits are mainly serially defeated by a 16 bit resolution, three lines of 2 passage fully differential simulation inputs The analog-digital converter AD7705 chips entered are realized.
AD7705 can be easily connected with microcontroller, also be compared using 3-line serial interface compatible SP I Q SP I Parallel interface mode greatlys save CPU I O mouths.AD7705 is complete 16 bit A/D converter.It employs sigma-delta skill Art, 16 can be obtained without number of bit errors according to output.This point meet very much it is higher to resolution requirement but to change numerical requirements Not high application, pass through external crystal oscillator, accurate a reference source and a small amount of decoupling capacitor, you can be carried out continuously A D conversions.It is logical Cross and be connected with REF195 and reference voltage can be made to bring up to 5V, reach requirement.
(5) liquid crystal display circuit
Liquid crystal display circuit uses QC12864B Chinese character image dot matrix lcd modules, can Display of Chinese characters and figure, it is interior 8192 Chinese characters (16*16 dot matrix), 128 characters (8*16 dot matrix) and 62*256 lattice display RAM64 dot matrix are put, point is big Small 0.48*0.48mm2, point spacing 0.04mm.This circuit design uses serial line interface, by being connected with single-chip microcomputer, realizes effective Control the correct display of liquid crystal display.Fig. 9 is liquid crystal display circuit figure.
(6) synchronous USB flash disk storage circuit
The storage circuit, the absorbance of the chlorophyll for storing the sample to be tested.What USB flash disk storage was selected is Nanjing The USB flash disk module of Qin Heng electronics corporations production is realized between single-chip microcomputer and memory module as storage device by MAX232 chips Communication.Figure 10 is serial port circuit and synchronous USB flash disk memory module.
Current signal I0 after being changed through photodetector, by I-U change-over circuits, current signal I0 is changed into voltage Signal U0, by the amplifying circuit being made up of LM358, voltage signal U0 is changed into U1, and some noise signals are now included in signal △ U, by the low-pass first order filter being made up of RC, noise signal △ U are removed, obtain U2 (U1- △ U).
Analog signal U2 is changed into data signal U3 by A/D change-over circuits, and data signal U3 is sent into single-chip microcomputer 89S52 processing Handled in device, final result is shown on 12864B liquid crystal displays, while is stored in by USB flash disk memory module in USB flash disk.Figure 11 is Electric signal processing process.
C, operational process designs
Software program, which is adopted, to be shown a C language, and mainly realizes light source control function, keyboard identification function, A/D translation functions, Software filtering function, data processing function, crystal display and USB flash disk store function, figure are main program flow chart, measurement procedure Figure and USB flash disk storage program flow chart.
The present invention's is simple to operate, is detector detailed operation and data processing method below.
1st, detection means job step
The first step, press【Power supply】Key, open system power supply, liquid crystal display:" in China Agricultural University's precision agriculture research Edema with the heart involved body chlorophyll content detector ", instrument initialized completion is represented, can be with normal work.
Second step, the sampling bottle of distilled water will be filled, be put into circular groove, press【Demarcation】Key, system will gather four successively Individual light intensity value, and it is included on liquid crystal display, the data collected are saved in USB flash disk by system automatically by Serial Port Transmission, Liquid crystal display " in data storage ".After the completion of data transfer, interface display " storage is completed ".If do not plug USB flash disk or USB flash disk does not have It is plugged, interface can prompt " please insert USB flash disk ".
3rd step, the sampling bottle of water sample will be filled, be put into circular groove, press【Measurement】Key, system will gather four successively Light intensity value, and it is included on liquid crystal display, it is automatically saved in USB flash disk.
4th step, after the light intensity value for collecting standard solvent and test solvent, its absorbance of system-computed and according to There is chlorophyll content forecast model, calculate the chlorophyll content of water sample, be shown on liquid crystal display and be saved in USB flash disk.
5th step, is reentered into sample, presses【Measurement】Key measures.
2 chlorophyll levels distribution map implementation methods
Water body chlorophyll content distribution figure is realized using VC method, can sit single-point water body chlorophyll content and GPS Mark information to combine, realize that single-point water body chlorophyll content is shown, interpolation, plot area measuring and calculating, cell water body chlorophyll content etc. It is worth line chart generation, decision-making foundation is provided for regulating and controlling water quality.
Said apparatus has advantages below:
1st, the in situ detection of chlorophyll concentration is realized
Using transmission beam method Cleaning Principle, the steps such as sampling in chlorophyll detection, pretreatment, purification are eliminated, at the same it is sharp Measure the mode of averaging respectively with upper and lower two-way, can effectively realize the in situ detection of water body Determination of Chlorophyll concentration.To water Water quality in production cultivation plays effective monitoring and directive function.
2nd, the change of monitoring chlorophyll concentration in real time
With reference to upper computer software, the chlorophyll concentration distribution map of different zones in waters can be generated in real time.In combination with Other meteorological and hydrographic informations, change and the trend of water body Determination of Chlorophyll concentration can be effectively predicted, so as to aquaculture Play positive directive function.
3rd, it is practical
The chlorophyll concentration distribution map that the present invention is generated by LCD screen and upper computer software can intuitively reflect water outlet Chlorophyll distribution in body region, effectively can intuitively carry out monitoring water quality and carry out regulating and controlling water quality.Can have for regulating and controlling water quality The offer decision-making foundation of effect.
In the specification of the present invention, numerous specific details are set forth.It is to be appreciated, however, that embodiments of the invention can be with Put into practice in the case of these no details.In some instances, known method, structure and skill is not been shown in detail Art, so as not to obscure the understanding of this description.
Finally it should be noted that:Various embodiments above is merely illustrative of the technical solution of the present invention, rather than its limitations;To the greatest extent The present invention is described in detail with reference to foregoing embodiments for pipe, it will be understood by those within the art that:Its according to The technical scheme described in foregoing embodiments can so be modified, either which part or all technical characteristic are entered Row equivalent substitution;And these modifications or replacement, the essence of appropriate technical solution is departed from various embodiments of the present invention technology The scope of scheme, it all should cover among the claim of the present invention and the scope of specification.

Claims (5)

  1. A kind of 1. detection method of chlorophyll content, it is characterised in that including:Using the chlorophyll solution of various concentrations, establish Chlorophyll detection model;
    Sample to be tested is detected using the incident light of two wavelength, obtains the absorbance of the chlorophyll of the sample to be tested, Described two wavelength are 663nm and 645nm;
    The absorbance of the chlorophyll of the sample to be tested is substituted into the chlorophyll detection model, obtains the leaf of the sample to be tested Green plain concentration:It is A=lg [I for the absorbance calculation formula under two wave bandss(λ)/Iz(λ)], wherein A is absorbance, Is (λ) be titer transmission value, Iz(λ) is the transmission of water sample;Maximum absorption band of the known chlorophyll a, b in red light district be respectively 663nm and 645nm, know again under wavelength 663nm, specific absorptivity of the chlorophyll a, b in the solution is respectively 82.04 Hes 9.27, it is respectively 16.75 and 45.6 under wavelength 645nm, relationship below is listed according to additive property principle:
    A663=82.04Ca+9.27Cb A645=16.75Ca+45.6Cb
    A663 and A645 is respectively absorbance of the chlorophyll solution in wavelength 663nm and 645nm in formula, and Ca, Cb are respectively leaf Green plain a, b concentration, unit mg/L;
    Solving equations can obtain:
    Ca=12.72A663-2.59A645 Cb=22.88A645-4.67A663
    Ca, Cb are respectively the concentration of chlorophyll a, b in formula, unit mg/L;Ca is added with Cb and produces chlorophyll total concentration
    CT=Ca+Cb=20.29A645+8.05A663,
    It will be located in model insertion single-chip microcomputer in detection means, for being directly invoked during measurement;
    Detection means is made up of opticator and control section, and opticator includes 4 LED light source emitters of 2 wave bands, It is powered by monolithic processor controlled light source driving circuit, is impinged perpendicularly on after collimation lens is collimated in water, by 4 Photodetector is gathered accordingly;Control section includes amplifying circuit, filter circuit, A/D change-over circuits, single-chip microcomputer control Circuit, liquid crystal display circuit and synchronous USB flash disk storage circuit;Detector is lighted successively using single-chip microcomputer control light source, light source hair The single band optical signal gone out is impinged perpendicularly in sample after collimation lens to be measured, by the absorption of sample, scattering and Transmission, is acquired, optical signal is converted into electric signal by photodetector by corresponding photodetector;Turn through photodetector The electric signal changed into is by amplifying, filtering, is kept in after A/D conversion process by 89S52 single-chip microcomputers, and the light with receiving successively Signal of change, which goes out after absorbance to substitute into water body chlorophyll content detection model together, to be calculated, and the result after calculating is by 128*64 Liquid crystal display exports, while realizes USB flash disk store function;
    According to low-power consumption, narrow bandwidth, the principle of portable principle and detector, the light emitting diodes of 2 different-wavebands is selected As incident light source, collimation lens impinges perpendicularly on the water of sample bottle as transmission channel after the collimated lens of light emitting diode In sample, gathered after the absorption, scattering and transmission of water sample by corresponding sensor to complete transformation of the optical signal to electric signal, This detector the upper and lower are same structure;
    The device that the detection method of the chlorophyll content uses includes:Model building module, photoelectric detection module and detection mould Block, the model building module are arranged in the detection module, and the photoelectric detection module is connected with the detection module;
    The model building module, for the chlorophyll solution using various concentrations, establish chlorophyll detection model;
    The photoelectric detection module, for being detected using the incident light of two wavelength to sample to be tested, obtain described to be measured The absorbance of the chlorophyll of sample;
    The detection module, for the absorbance of the chlorophyll of the sample to be tested to be substituted into the chlorophyll detection model, obtain Take the concentration of the chlorophyll of the sample to be tested.
  2. 2. according to the method for claim 1, it is characterised in that the detection module includes:Voltage conversion circuit, light source are steady Current circuit, I-U change-over circuits, filter amplification circuit, A/D change-over circuits, display circuit and storage circuit.
  3. 3. according to the method for claim 2, it is characterised in that the voltage conversion circuit, for being given by voltage conversion The detection module power supply.
  4. 4. according to the method for claim 2, it is characterised in that the light source current stabilization circuit, for light source power supply.
  5. 5. according to the method for claim 2, it is characterised in that the storage circuit, for storing the sample to be tested The absorbance of chlorophyll.
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