CN109238993A - The detection method that Determination of Chlorophyll In Seawater content influences underwater optical transmission characteristics - Google Patents
The detection method that Determination of Chlorophyll In Seawater content influences underwater optical transmission characteristics Download PDFInfo
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- CN109238993A CN109238993A CN201811430223.6A CN201811430223A CN109238993A CN 109238993 A CN109238993 A CN 109238993A CN 201811430223 A CN201811430223 A CN 201811430223A CN 109238993 A CN109238993 A CN 109238993A
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/3103—Atomic absorption analysis
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
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Abstract
The invention discloses a kind of detection methods that Determination of Chlorophyll In Seawater content influences underwater optical transmission characteristics, sample, spectral detection, spectrum analysis are prepared, compares the intensity of the absorption spectra of the full sea water sample and the seawater sample and the standard absorption peak of transmission spectrum and transmission peaks respectively；The average value for taking spectrum analysis calculates chlorophyll content to the absorptivity and transmissivity of spectrum.Present invention employs the methods of UV, visible light near infrared spectrometer, the absorption spectra and transmission spectrum of sample are measured respectively, the spectral characteristic for analyzing without chlorophyll seawater sample solution and being added chlorophyll seawater sample solution, to obtain influence of the Determination of Chlorophyll In Seawater content for underwater optical transmission characteristics.Accuracy is high, and effect is obvious, chlorophyll content can be precisely calculated to the absorptivity and transmissivity of spectrum, can detect Determination of Chlorophyll In Seawater content influences underwater optical transmission characteristics.
Description
Technical field
The present invention relates to spectrographic detection technology, in particular to a kind of Determination of Chlorophyll In Seawater content is for underwater optical transmission characteristics
The detection method of influence.
Background technique
Currently, seawater pollution problem, than more serious, seawater muddiness caused by marine pollution seriously affects sea-plant and (swims
Plant and seaweed) photosynthesis, to influence the productivity in sea area.Due to containing a large amount of phytoplanktons in seawater, they are
The primary product of light-use.The chlorophyll wherein contained can convert light energy into biological energy source, this is to influence light in seawater
One essential condition of absorption characteristic, so, the content of chlorophyll has a very big impact underwater light propagation.Due to chlorophyll
The scattering of light can be influenced, someone uses the experiment proves that had an impact Phytoplankton Photosynthesis In The Pohai and chlorophyll concentration
The factor can all influence primary productivity of marine ecosystem, so, one kind probing into Determination of Chlorophyll In Seawater content for underwater optical transmission characteristics shadow
Loud method is to get a good eye value.
Currently, there are many influence factor that influence light transmits in the seawater, ocean temperature, salinity, pressure, turbulent flow etc., still
Influence of the content to optical transport for phytoplankton in seawater, especially the content of simulated seawater Determination of Chlorophyll is for optical transport
Influence really rarely have research.Absorption of the chlorophyll content to 200-1200nm range of spectra under effective simulation real sea water environment
With scattering situation, to optical transport in seawater, optic communication has far-reaching influence.
Summary of the invention
Probe into what Determination of Chlorophyll In Seawater content influenced underwater optical transmission characteristics the purpose of the present invention is to provide a kind of
Method analyzes influence of the different depth chlorophyll content to optical transmission characteristics in seawater by optical spectrum detecting method.
The purpose of the present invention is realized using following technical scheme.Determination of Chlorophyll In Seawater content is for underwater optical transmission characteristics
The detection method of influence, its step are as follows:
1) it prepares sample: taking full sea water sample in cuvette, various concentration chlorophyll is respectively configured according to sea water advanced
Seawater sample；
2) spectrum inspection spectral detection: is carried out to the seawater sample using UV, visible light near infrared spectrometer
It surveys；
3) spectrum analysis: standard absorption peak and the transmission of the full sea water sample and the seawater sample are compared respectively
The intensity at peak, if identical, the chlorophyll content in seawater is on underwater optical transmission characteristics without influence；If it is different, then seawater
In chlorophyll content have an impact to underwater optical transmission characteristics；
4) average value for taking step 3) spectrum analysis calculates chlorophyll content to the absorptivity and transmissivity of spectrum.
Further, the salinity of the full sea water sample is 3~4%；
Further, it is 3.7mg/m that the chlorophyll content configuration of the seawater sample, which is respectively as follows: seawater surface,3；10 meters
Deep-sea water is 6.72mg/m3；15 meters of deep-sea water are 5.5mg/m3；20 meters of deep-sea water are 3mg/m3；
Further, the gap of the spectrographic detection is 7~9nm；
Further, the range of the spectrographic detection wavelength is 200nm-1400nm.
Present invention employs the methods of spectrophotometer, configure achlorophyllous seawater standard sample solution to be measured and addition
The seawater sample standard solution of various concentration chlorophyll measures the absorption spectra and transmission spectrum of sample respectively, and analysis is without chlorophyll sea
Sample solutions and the spectral characteristic that chlorophyll seawater sample solution is added, to obtain Determination of Chlorophyll In Seawater content for underwater
The influence of optical transmission characteristics.It needs to be repeated as many times in test to reduce error in the method being averaged, has studied chlorophyll and contain
The scattering and absorption to the light of 200nm-1400nm spectrum are measured, experimental spectrum range is wide, and accuracy is high, and effect is obvious, can be accurate
Ground calculates chlorophyll content to the absorptivity and transmissivity of spectrum, can detect Determination of Chlorophyll In Seawater content and Underwater Optical is passed
Broadcast characteristic influence.
Detailed description of the invention
Fig. 1 is detection device schematic diagram of the invention；
In figure: 100- UV, visible light near infrared spectrometer, 1-LED light source, the first convex lens of 2-, 3- pinhole filter
Device, 4- concavees lens, 5- slit, the second convex lens of 6-, 7- cuvette；8- signal receiving processing system, 9- computer.
Specific embodiment
Below in conjunction with drawings and examples, the invention will be further described.Referring to Fig. 1, Determination of Chlorophyll In Seawater of the present invention contains
UV, visible light near infrared spectrometer 100 in the detection method for influencing underwater optical transmission characteristics is measured to be issued by LED light source 1
Light beam passes through the collimation filtering system being made of the first convex lens 2, pinhole filter 3 and concavees lens 4, becomes directional light entrance point
Light grating slit 5 enters in article colorimetric ware 7 using the light beam for being divided into different wave length after the second convex lens 6, and compares
Color ware 7 passes through 9 connection signal receiving processing system 8 of computer.
Its testing procedure is as follows:
1. sample preparation configures the seawater standard solution of 3.5% salinity with sea salt first, then configuration and sample to be tested salt
Seawater sample that is identical but having added various concentration chlorophyll is spent, is then measured with UV, visible light near infrared spectrometer to test sample
The absorption spectra and transmission spectrum of product, the absorption peak and transmission peaks and Strength Changes situation of analysis absorption spectrum and projection spectrum.
2. no chlorophyll seawater sample solution salinity is 3.5%, the seawater sample simulation In The Western Part of The East China Sea edge of chlorophyll is added
It is green to be arranged respectively at addition leaf in 3.5% salinity seawater standard solution for the chlorophyll depth concentration levels of the true water body in bank area
Plain concentration is seawater surface: 3.7mg/m3；10 meters of deep-sea water: 6.72mg/m3；15 meters of deep-sea water: 5.5mg/m3；20 meters of deep-sea water:
3mg/m3These four concentration.
3. using LED light source 1, the first convex lens 2, pinhole filter 3, concavees lens 4, the slit 5, second set gradually
The UV, visible light near infrared spectrometer 100 that convex lens 6 and cuvette 7 are constituted, wherein cuvette 7 passes through computer 9 and letter again
Number receiving processing system 8 connects, and computer 9 uses UVProbe2.33 software.
4. firstly, the absorption spectra and transmission of seawater sample solution of the comparison without chlorophyll seawater sample and containing chlorophyll
Spectrum, if it is identical, then the standard absorption peak and transmission peaks of absorption spectra and transmission spectrum and the intensity at peak are compared, if identical,
Chlorophyll content in seawater is on underwater optical transmission characteristics without influence.If it is different, then the chlorophyll content in seawater is to underwater
Optical transmission characteristics have an impact.
The operation principle of the present invention is that: configuration sample to be tested opens computer 9 and UV, visible light near infrared spectrometer
100 work normally；Configure the seawater sample without chlorophyll seawater sample and containing various concentration chlorophyll；It is close by UV, visible light
Infrared spectrophotometer 100 measures absorption spectra and transmission spectrum and Strength Changes；It analyzes absorption spectrum and projects the absorption of spectrum
Peak and transmission peaks and Strength Changes situation obtain the detection comparing result of sample detection.
Claims (5)
1. the detection method that Determination of Chlorophyll In Seawater content influences underwater optical transmission characteristics, which is characterized in that its step are as follows:
1) it prepares sample: taking full sea water sample in cuvette, according to the sea water advanced sea that various concentration chlorophyll is respectively configured
Water sample；
2) spectral detection spectral detection: is carried out to the seawater sample using UV, visible light near infrared spectrometer；
3) spectrum analysis: the standard absorption peak and transmission peaks of the full sea water sample and the seawater sample are compared respectively
Intensity, if identical, the chlorophyll content in seawater is on underwater optical transmission characteristics without influence；If it is different, then in seawater
Chlorophyll content has an impact to underwater optical transmission characteristics；
4) average value for taking step 3) spectrum analysis calculates chlorophyll content to the absorptivity and transmissivity of spectrum.
2. the detection method that Determination of Chlorophyll In Seawater content according to claim 1 influences underwater optical transmission characteristics,
It is characterized in that, the salinity of the full sea water sample is 3~4%.
3. the detection method that Determination of Chlorophyll In Seawater content according to claim 1 influences underwater optical transmission characteristics,
It is characterized in that, it is 3.7mg/m that the chlorophyll content configuration of the seawater sample, which is respectively as follows: seawater surface,3；10 meters of deep-sea water
For 6.72mg/m3；15 meters of deep-sea water are 5.5mg/m3；20 meters of deep-sea water are 3mg/m3。
4. the detection method that Determination of Chlorophyll In Seawater content according to claim 1 influences underwater optical transmission characteristics,
It is characterized in that, the gap of the spectrographic detection is 7~9nm.
5. the detection method that Determination of Chlorophyll In Seawater content according to claim 1 influences underwater optical transmission characteristics,
It is characterized in that, the range of the spectrographic detection wavelength is 200nm-1400nm.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113746544A (en) * | 2021-09-09 | 2021-12-03 | 桂林电子科技大学 | Vertical channel link layering method for non-uniform seawater |
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