CN102023136A - Method for detecting purple sweet potato anthocyanidin pigment by ultraviolet spectrophotometry - Google Patents
Method for detecting purple sweet potato anthocyanidin pigment by ultraviolet spectrophotometry Download PDFInfo
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- CN102023136A CN102023136A CN 201010179143 CN201010179143A CN102023136A CN 102023136 A CN102023136 A CN 102023136A CN 201010179143 CN201010179143 CN 201010179143 CN 201010179143 A CN201010179143 A CN 201010179143A CN 102023136 A CN102023136 A CN 102023136A
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Abstract
The invention relates to a detection method, in particular to a method for detecting a purple sweet potato anthocyanidin pigment by ultraviolet spectrophotometry. The method comprises the following steps of: a, preparing purple sweet potato anthocyanidin pigment extract; b, adjusting the purple sweet potato anthocyanidin pigment extract to be acidic; and c, under the maximum absorption wavelength of the purple sweet potato anthocyanidin pigment, detecting the absorbance of anthocyanidin pigment solution to be detected. The detection method is easy to operate, convenient to compute, and accurate in result, has low cost of used instruments and low detection cost, and is suitable for the frontline of production; meanwhile, the detection method is special for the purple sweet potato anthocyanidin pigment, and the invention provides an economic, visual and rapid qualitative and quantitative test for purple sweet potato anthocyanidin pigment production enterprises.
Description
Technical field
The present invention relates to a kind of detection method, be specifically related to the method that a kind of ultraviolet-spectrophotometric method detects purple sweet potato anthocyania pigment.
Background technology
Natural purple sweet potato anthocyania pigment has many advantages: nontoxic, have no side effect, safe; Tone is more natural, approaches natural materials; Raw material sources are convenient, and are cheap; Technology is simple, and extraction cost is low; Soluble in water, the good stability in beverage, and the lipotropism oxidisability is strong; Have multiple nutrients, pharmacology and health care.Therefore purple sweet potato anthocyania pigment all has value widely in food, chemical industry, cosmetics and pharmaceuticals industry.The extraction of the purple sweet potato anthocyania pigment of research, purifying and functional character is more at present, but less to the research of detection method, the method for its detection generally is the detection method of prolonging with other pigments, does not have unified GB to come standard.Because different pigment character are totally different, apply mechanically the detection method of other pigment and can bring many deviations and inconvenience to the production of purple sweet potato anthocyania pigment.
Existing pigment check and analysis method has high performance liquid chromatography and thin-layered chromatography: the former sensitivity and accuracy height, but expensive high can not the popularizing of instrument; Latter's sensitivity is low, poor accuracy.For the mensuration of pigment content, way commonly used both at home and abroad is to be that primary standard substance calculates its absolute content with synthetic amaranth.This method can reflect the pigment total content more exactly, but it needs standard model, is not suitable for factory of basic unit and uses.Therefore, in order to adapt to the needs that purple sweet potato anthocyania pigment is produced, how making a kind of special detection method at purple sweet potato anthocyania pigment becomes the urgent demand of people.
Summary of the invention
The object of the present invention is to provide a kind of specially at the detection method of purple sweet potato anthocyania pigment, for purple sweet potato anthocyania pigment manufacturing enterprise provide economical, intuitively, qualitative and quantitative measurement fast.
The objective of the invention is to realize by following scheme: a kind of ultraviolet-spectrophotometric method detects the method for purple sweet potato anthocyania pigment, it is characterized in that: this method may further comprise the steps:
A, the purple sweet potato anthocyania pigment extract of preparation;
B, purple sweet potato anthocyania pigment extract is adjusted to acidity;
C, under the maximum absorption wavelength of sweet potato anthocyania pigment, anthocyania pigment solution to be measured is carried out absorbance detection.
The method that described ultraviolet-spectrophotometric method detects purple sweet potato anthocyania pigment also comprises the mensuration of the maximum absorption wavelength of sweet potato anthocyania pigment in the described b step solution.This step is in order to determine maximum absorption wavelength, can also to qualitatively judge simultaneously.
The method that described ultraviolet-spectrophotometric method detects purple sweet potato anthocyania pigment also comprises the drawing standard curve, determines the range of linearity of absorbance.The effect of this step is should improve or reduce concentration if find that the absorbance of liquid to be measured is too little or too very much not among the range of linearity time, and absorbance is adjusted within this range of linearity, guarantees to measure accurately.
Described drawing standard curve, the range of linearity of determining absorbance is for to prepare the purple sweet potato anthocyania pigment solution of different quality concentration in beaker respectively, regulate this purple sweet potato anthocyania pigment solution and get the pH value, this purple sweet potato anthocyania pigment solution gets the pH value and equates with the pH value of solution in the described b step, is transferred in the volumetric flask then and constant volume; Get the sample of equivalent respectively from above-mentioned volumetric flask, the sodium sulphite that adds capacity, leave standstill, make the pigment full bleaching, respectively as the blank sample of the purple sweet potato anthocyania pigment of respective concentration solution, under the maximum absorption wavelength that records in step c sweet potato anthocyania pigment extract is fixed a point to detect, the drawing standard curve is determined the range of linearity of absorbance.
PH value with purple sweet potato anthocyania pigment extract in the described b step is adjusted to 1-3.
PH value with purple sweet potato anthocyania pigment extract in the described b step is adjusted to 3.
In described e step, described anthocyania pigment solution absorbency detects carries out under 10-60 ℃.
The invention has the advantages that: because material has selectivity to the absorption of light, so different food coloring has different absorption spectrograms.Utilize this principle, use ultraviolet-visible spectrophotometer purple sweet potato anthocyania pigment is carried out absorption spectrum scanning, and with the contrast of standard spectrogram, can be intuitively, qualitative fast.And under purple sweet potato anthocyania pigment maximum absorption band wavelength, measure the absorbance of quantitative sample, and can try to achieve the look valency, thereby determine the content of pigment in the sample, sensitivity and accuracy are all higher.Detection method of the present invention is simple to operate, calculating is easy, the result is obvious, used instrument low price, detects with low costly, and use suitable production line.Be a kind of specially at the detection method of purple sweet potato anthocyania pigment, for purple sweet potato anthocyania pigment manufacturing enterprise provide economical, intuitively, qualitative and quantitative measurement fast.
Description of drawings
Fig. 1 is the purple sweet potato anthocyania pigment solution of the present invention uv absorption spectra when pH=3;
Fig. 2 detects the influence of the purple potato pigment concentration of method of purple sweet potato anthocyania pigment to linear relationship for ultraviolet-spectrophotometric method of the present invention.
Embodiment
The present invention will be further described below in conjunction with accompanying drawing.
As shown in Figure 1, a kind of ultraviolet-spectrophotometric method detects the method for purple sweet potato anthocyania pigment, this method may further comprise the steps: a, the purple sweet potato anthocyania pigment extract of preparation, the purple sweet potato powder end is dissolved, precision takes by weighing pigment sample 5g to be measured, separate and be settled to 50ml with pH value=6 water-soluble, make storing solution; B, adjusting pH value, get the 10ml storing solution, regulate pH to 3, be transferred to then in the volumetric flask of 100mL with the HCl of 0.1mol/L and the NaOH of 0.1mol/L, the buffer solution that with pH is sodium hydrogen phosphate-citric acid of 3 is settled to 100ml, ready to balance after 2 hours as extract to be measured; C, under the maximum absorption wavelength of sweet potato anthocyania pigment, anthocyania pigment solution to be measured is carried out absorbance detection.
The method that ultraviolet-spectrophotometric method detects purple sweet potato anthocyania pigment also comprises the mensuration of the maximum absorption wavelength of sweet potato anthocyania pigment in the b step solution.When not knowing the maximum absorption wavelength of purple sweet potato anthocyania pigment under a certain pH value, measure by this step.
The method that ultraviolet-spectrophotometric method detects purple sweet potato anthocyania pigment also comprises the drawing standard curve, determine the range of linearity of absorbance, this step be specially prepare different quality concentration respectively purple sweet potato anthocyania pigment solution in beaker, regulate this purple sweet potato anthocyania pigment solution and get the pH value, this purple sweet potato anthocyania pigment solution gets the pH value and equates with the pH value of solution in the described b step, is transferred in the volumetric flask then and constant volume; Get the sample of equivalent respectively from above-mentioned volumetric flask, the sodium sulphite that adds capacity, leave standstill, make the pigment full bleaching, respectively as the blank sample of the purple sweet potato anthocyania pigment of respective concentration solution, under the maximum absorption wavelength that records in step c sweet potato anthocyania pigment extract is fixed a point to detect, the drawing standard curve is determined the range of linearity of absorbance.
EXPERIMENTAL EXAMPLE 1: get the purple sweet potato anthocyania pigment of 10ml extract respectively in 6 beakers, regulate pH to 1,2,3,4,5,6 with the HCl of 0.1mol/L and the NaOH of 0.1mol/L, being transferred to then in the volumetric flask of 100mL, is that the buffer solution of sodium hydrogen phosphate-citric acid of 1,2,3,4,5,6 is settled to 100ml with pH respectively.Treating its balance 2h, is that blank sample detects with distilled water.In the wavelength coverage of 400-600nm, scan, obtain maximum absorption wavelength and obtained the maximum absorption under the different pH values.The absorbing state of purple sweet potato anthocyania pigment under different pH values of same concentrations sees Table 1.
Table 1pH value is to the influence of purple sweet potato anthocyania pigment absorbance and maximum absorption wavelength
As can be seen from Table 1, along with the decline of pH value, the absorbance of purple sweet potato anthocyania pigment increases.In the pH value is 1,2,3 o'clock, and purple sweet potato anthocyania pigment absorbance is approaching.When the pH value was 3.0, the stable better and while of purple sweet potato anthocyania pigment can be got rid of the influence of interference.
EXPERIMENTAL EXAMPLE 2: be 3 purple sweet potato anthocyania pigment solution water bath with thermostatic control heating 30min with the pH value for preparing in different temperatures, measure its absorbance at the 528nm place, absorbance does not have significant change when finding 10～60 ℃, and absorbance reduces gradually during greater than 60 ℃.Experimental result shows that the stability of pigment descends gradually along with the rising of heating temperature and the prolongation of heated time, and stability is arranged under the room temperature acid condition preferably, avoids high temperature in the operating process and is heated for a long time.
The mensuration of the maximum absorption wavelength of sweet potato anthocyania pigment is specially that preparation pH is 3 in the embodiment 3:b step solution, pigment concentration is 1% solution, carries out UV scanning in the 400-600nm scope, absorption spectrum, as shown in Figure 2.As can be seen from Figure 2, equal at 3 o'clock in the pH value, the maximum absorption wavelength of purple sweet potato anthocyania pigment is 528 ± 1nm.
Embodiment 4: the drawing standard curve, the range of linearity of determining absorbance is specially the purple sweet potato anthocyania pigment extract 2,4,6,8,10,12,14,16,18,20,22,24,26,28 of getting concentration known respectively, 30mL in the 15g beaker, NaOH with the hydrochloric acid of 0.1mol/L and 0.1mol/L is regulated pH to 3.0, is transferred in the volumetric flask of 100mL then and is settled to 100mL; Get the 20mL sample from above-mentioned volumetric flask respectively, add the sodium sulphite of capacity, leave standstill 30min and make the pigment full bleaching, respectively as corresponding blank sample; Under 528 ± 1nm wavelength, fix a point to detect, to curve as shown in Figure 3, determine the range of linearity of absorbance.
As shown in Figure 3, less than 22%, absorbance was less than 2.9 o'clock in purple sweet potato anthocyania pigment extract mass concentration, and purple sweet potato anthocyania pigment mass concentration and absorbance have linear relationship, and promptly the size of absorbance is directly proportional with the concentration of pigment.When absorbance can reduce error greater than 0.2 the time, therefore purple potato pigment absorbance detection model a side gate of an imperial palace is 0.2-3.1.
EXPERIMENTAL EXAMPLE 5: get 10mL purple sweet potato anthocyania pigment solution to be detected, add the 0.3g sodium sulphite, bleaching 10min, the pattern spare solution after the bleaching is as the blank sample that detects.At wavelength 528nm anthocyania pigment solution is carried out absorbance detection, and calculate the look valency.
Wherein, the quality of the cumulative volume W-solid sample of A-extract absorbance V-extract.
Table 2 Precision test result
The purple sweet potato red natural coloring matter solution of preparation 2%, 6%, 11% 3 kind of concentration carries out 6 replicate determinations according to method of operating, the homochromatic valency of making even, calculate RSD, the result as shown in Table 2, the RSD scope is between 0.36%-1.99%, RSD mean value is 1.07%, and test accuracy height is described.
Table 3 recovery test result
According to the range of linearity of typical curve, get 1/2 of basic, normal, high concentration value and make former content, be respectively C1, C2, C3; Each former content adds the amount of C1, C2, C3 more respectively to be done to add scalar, measures by method then.The result as shown in Table 3, purple sweet potato red natural coloring matter recovery of standard addition illustrates this method degree of accuracy height between 95%-99%.
Present embodiment only is the preferred embodiments of the invention in sum; unintelligible is qualification to protection domain of the present invention; for technical work personnel present embodiment is done according to the present invention the adjustment that does not exceed technical solution of the present invention and the change in this field, should think and drop in protection scope of the present invention.
Claims (7)
1. a ultraviolet-spectrophotometric method detects the method for purple sweet potato anthocyania pigment, and it is characterized in that: this method may further comprise the steps:
A, the purple sweet potato anthocyania pigment extract of preparation;
B, purple sweet potato anthocyania pigment extract is adjusted to acidity;
C, under the maximum absorption wavelength of sweet potato anthocyania pigment, anthocyania pigment solution to be measured is carried out absorbance detection.
2. detect the method for purple sweet potato anthocyania pigment according to the described ultraviolet-spectrophotometric method of claim 1, it is characterized in that: the method that described ultraviolet-spectrophotometric method detects purple sweet potato anthocyania pigment also comprises the mensuration of the maximum absorption wavelength of sweet potato anthocyania pigment in the described b step solution.
3. detect the method for purple sweet potato anthocyania pigment according to claim 1 or 2 described ultraviolet-spectrophotometric method, it is characterized in that: the method that described ultraviolet-spectrophotometric method detects purple sweet potato anthocyania pigment also comprises the drawing standard curve, determines the range of linearity of absorbance.
4. detect the method for purple sweet potato anthocyania pigment according to any described ultraviolet-spectrophotometric method of claim in the claim 3, it is characterized in that: described drawing standard curve, the range of linearity of determining absorbance is for to prepare the purple sweet potato anthocyania pigment solution of different quality concentration in beaker respectively, regulate this purple sweet potato anthocyania pigment solution and get the pH value, this purple sweet potato anthocyania pigment solution gets the pH value and equates with the pH value of solution in the described b step, is transferred in the volumetric flask then and constant volume; Get the sample of equivalent respectively from above-mentioned volumetric flask, the sodium sulphite that adds capacity, leave standstill, make the pigment full bleaching, respectively as the blank sample of the purple sweet potato anthocyania pigment of respective concentration solution, under the maximum absorption wavelength that records in step c sweet potato anthocyania pigment extract is fixed a point to detect, the drawing standard curve is determined the range of linearity of absorbance.
5. detect the method for purple sweet potato anthocyania pigment according to the described ultraviolet-spectrophotometric method of claim 1, it is characterized in that: the pH value with purple sweet potato anthocyania pigment extract in the described b step is adjusted to 1-3.
6. detect the method for purple sweet potato anthocyania pigment according to the described ultraviolet-spectrophotometric method of claim 5, it is characterized in that: the pH value with purple sweet potato anthocyania pigment extract in the described b step is adjusted to 3.
7. detect the method for purple sweet potato anthocyania pigment according to the described ultraviolet-spectrophotometric method of claim 1, it is characterized in that: in described e step, described anthocyania pigment solution absorbency detects carries out under 10-60 ℃.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102221533A (en) * | 2011-06-09 | 2011-10-19 | 西北农林科技大学 | Method for quantitatively detecting adulteration of peanut oil based on ultraviolet spectrum |
| CN102507485A (en) * | 2011-10-18 | 2012-06-20 | 山东农业大学 | Nondestructive testing method for pigment content of color wheat grain |
| CN104062246A (en) * | 2014-06-06 | 2014-09-24 | 中国计量学院 | Method for detecting mirabilite in sodium cyclamate by using ultraviolet visible spectrophotometry |
| RU2557953C2 (en) * | 2013-12-11 | 2015-07-27 | Государственное бюджетное образовательное учреждение высшего профессионального образования "Самарский государственный медицинский университет" Министерства здравоохранения Российской Федерации | Method for measuring anthocyanes in crude drugs |
| CN108918449A (en) * | 2018-07-13 | 2018-11-30 | 河南工业大学 | A kind of paddy xanthochromia degree detection method based on UV-VIS spectrophotometry |
| CN109238993A (en) * | 2018-11-28 | 2019-01-18 | 南昌航空大学 | The detection method that Determination of Chlorophyll In Seawater content influences underwater optical transmission characteristics |
| CN109323982A (en) * | 2018-11-16 | 2019-02-12 | 浙江万里学院 | Many condition controls natural pigment Stability Determination device and application method |
| CN114354523A (en) * | 2022-01-08 | 2022-04-15 | 杭州市农业科学研究院 | Rapid comparison method for content of procyanidine in radix tetrastigme leaves of different provenances |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102221533A (en) * | 2011-06-09 | 2011-10-19 | 西北农林科技大学 | Method for quantitatively detecting adulteration of peanut oil based on ultraviolet spectrum |
| CN102221533B (en) * | 2011-06-09 | 2012-08-08 | 西北农林科技大学 | Method for quantitatively detecting adulteration of peanut oil based on ultraviolet spectrum |
| CN102507485A (en) * | 2011-10-18 | 2012-06-20 | 山东农业大学 | Nondestructive testing method for pigment content of color wheat grain |
| RU2557953C2 (en) * | 2013-12-11 | 2015-07-27 | Государственное бюджетное образовательное учреждение высшего профессионального образования "Самарский государственный медицинский университет" Министерства здравоохранения Российской Федерации | Method for measuring anthocyanes in crude drugs |
| CN104062246A (en) * | 2014-06-06 | 2014-09-24 | 中国计量学院 | Method for detecting mirabilite in sodium cyclamate by using ultraviolet visible spectrophotometry |
| CN108918449A (en) * | 2018-07-13 | 2018-11-30 | 河南工业大学 | A kind of paddy xanthochromia degree detection method based on UV-VIS spectrophotometry |
| CN109323982A (en) * | 2018-11-16 | 2019-02-12 | 浙江万里学院 | Many condition controls natural pigment Stability Determination device and application method |
| CN109323982B (en) * | 2018-11-16 | 2021-01-15 | 浙江万里学院 | Multi-condition control natural pigment stability measuring device and using method |
| CN109238993A (en) * | 2018-11-28 | 2019-01-18 | 南昌航空大学 | The detection method that Determination of Chlorophyll In Seawater content influences underwater optical transmission characteristics |
| CN114354523A (en) * | 2022-01-08 | 2022-04-15 | 杭州市农业科学研究院 | Rapid comparison method for content of procyanidine in radix tetrastigme leaves of different provenances |
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Application publication date: 20110420 |