CN108279225A - Phytoplankton ＆ Suspension red tide and its toxicity detection optics home position sensing - Google Patents
Phytoplankton ＆ Suspension red tide and its toxicity detection optics home position sensing Download PDFInfo
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- CN108279225A CN108279225A CN201810132036.3A CN201810132036A CN108279225A CN 108279225 A CN108279225 A CN 108279225A CN 201810132036 A CN201810132036 A CN 201810132036A CN 108279225 A CN108279225 A CN 108279225A
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- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
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- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N2021/6417—Spectrofluorimetric devices
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
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- G01N2021/6471—Special filters, filter wheel
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Abstract
The invention discloses a kind of Phytoplankton ＆ Suspension red tide and its toxicity detection optics home position sensings, including：Light source module, reference light detection module, sample cell and scattering light detection module, reflected fluorescent light detection module and signal driving and processing module；Signal drives provides monochromatic ultraviolet light with processing module driving light source module for algae red tide and toxicity detection, and monochromatic ultraviolet light is divided into reference light and detection light by reference light detection module, and is detected to reference light, and detectable signal is compensated for reference background light；Detection light enters sample cell and scattering light detection module, irradiates seawater to be detected, the fluorescence being reflected back is detected via reflected fluorescent light detection module；Detection signal and detectable signal are sent to signal driving and processing module, realize the inside self compensation of algae red tide and toxicity detection sensor, complete the measurement of Phytoplankton ＆ Suspension red tide and toxicity.Present device is small and exquisite, integrated level is high, it can be achieved that on-line measurement in situ, accuracy of detection are high.
Description
Technical field
The invention belongs to algae red tide and toxicity detection technical fields, are related to a kind of seawater algae based on three-dimensional fluorescence spectrum
Class red tide and its toxicity detection optics home position sensing.
Background technology
Red tide (harmful algal bloom, HAB), also known as red tide, are certain in seawater under certain environmental conditions
A little phytoplanktons, protozoan or bacterium burst proliferation or high aggregation and cause the harmful ecology of one kind of water color-changing existing
As.Red tide can make the aquatic animals such as fish cause machine this is because red tide planktonic organism blocks the fish gill by very big harm sometimes
Tool obstacle, they are after death decomposed, and consume oxygen rapidly, and water oxygen is insufficient, and caused by secretion harmful substance etc..Fish toxicity
(Ichthyotoxic) red tide can generate a variety of noxious materials, cause fish and shellfish mortality in a short time.Fish toxicity
Red tide algae has been distributed widely in China coast, seriously polluted China's mariculture environment, restricts China coast seawater and supports
Grow the sustainable and healthy development of industry.
The detection method of Phytoplankton ＆ Suspension red tide and toxicity includes mainly following three classes at present：First, it is based on pigment spectroscopic methodology,
Second is that being based on fluorescent spectrometry, three, which are utilized in line, visualizes unicellular technique for taking.Since the absorption spectrum of phytoplankton is easy
It is influenced by yellow substance and light protection pigment, to limit the further development of pigment spectroscopic methodology.With fluorescence in recent years
The progress of detection technique and image analyzing and processing technology, three-dimensional fluorescence spectrum technology and the unicellular technique for taking of visualization are in algae
It is rapidly developed in terms of identification, and three-dimensional fluorescence spectrum technology is increasingly mature, has developed a variety of oil, more of being applied at present
The professional equipment of chlordiphenyl, bio-hormone and microalgae identification etc..Chinese patent (patent No. CN201410648240.2) is public
A kind of construction method in the three-dimensional fluorescence standard spectrum library of the algae of fish toxicity for identification is opened, this method passes through fish toxicity algae
The research of hemotoxin and chlorophyll three-dimensional fluorescence spectrum under different growing stage and environmental factor regulation and control, by cluster side
Method establishes three-dimensional fluorescence standard spectrum library, for identifying fish toxicity algae and its toxicity size.
Invention content
(1) technical problems to be solved
The technical problem to be solved by the present invention is to：A kind of Phytoplankton ＆ Suspension red tide is provided and its toxicity detection optics senses in situ
Device obtains algae chlorophyll concentration and hemotoxin three-dimensional fluorescence spectrum, and realization automatically determines and data processing, realizes seawater algae
The automatic on-line in situ detection of class red tide and toxicity achievees the effect that rapid reaction, non-maintaining, autonomous operation.
(2) technical solution
In order to solve the above technical problem, the present invention provides a kind of Phytoplankton ＆ Suspension red tide and its toxicity detection optics to pass in situ
Sensor comprising：Light source module, reference light detection module, sample cell and scattering light detection module, reflected fluorescent light detection module and
Signal drives and processing module；Signal drives provides monochrome with processing module driving light source module for algae red tide and toxicity detection
Ultraviolet light, monochromatic ultraviolet light is divided into reference light and detection light by reference light detection module, and is detected to reference light, detection letter
Number for reference background light compensate；Detection light enters sample cell and scattering light detection module, irradiates seawater to be detected, is reflected back
Fluorescence is detected via reflected fluorescent light detection module；Detection signal and detectable signal are sent to signal driving and processing module, real
The inside self compensation of existing algae red tide and toxicity detection sensor, completes the measurement of Phytoplankton ＆ Suspension red tide and toxicity.
Wherein, the light source module includes LED array 2 and adjustable filter array 3, and the connection of 2 output end of LED array linearly may be used
Filter array 3 is adjusted, is monochromatic ultraviolet light by output ultraviolet light filter.
Wherein, the reference light detection module includes spectroscope 4 and optical detector 11, and spectroscope 4 is located at monochromatic ultraviolet light
On emitting light path, minute surface is in 45° angle with monochromatic ultraviolet light exit direction, and monochromatic ultraviolet light after spectroscope 4, make by the light beam of transmission
To detect light, the light beam of reflection, which is used as, refers to light, and optical detector 11 is located in reference light light path, and detectable signal is used for reference background
Light compensates.
Wherein, the spectroscope 4 is 10~30% to the reflectivity of monochromatic ultraviolet light.
Wherein, the sample cell and scattering light detection module include binary transflector mirror 5, first collimator 6, sample cell 7,
Binary transflector mirror 6 is located on light source emitting light path after spectroscope 4, and minute surface is in 45° angle with light source direction of illumination, in its reflection
First collimator 6, sample cell 7, the cloth between binary transflector mirror 5 and sample cell 7 of first collimator 6 are sequentially arranged in light light path
It sets, ultraviolet light is converged in sample cell 7, seawater to be detected is held in sample cell 7.
Wherein, the reflected fluorescent light detection module includes the second collimator 8, diffraction grating 9, electron multiplication CCD10, and second
Collimator 8 and diffraction grating 9 be located at 7 opposite side of first collimator 6 and sample cell, is reflected back from sample cell 7 for collecting
Fluorescence, reflected fluorescent light are collimated through the second collimator 8, and diffraction grating 9 will be launched by the reflected fluorescent light of collimation by wave arrangement
Spectrum, electron multiplication CCD10 is located at the reflectance spectrum position of the formation of diffraction grating 9, the optical signal of fluorescence spectrum changed
At electric signal.
Wherein, the operation principle of the sensor is：The linear adjustable filter array of light that LED array 2 is sent out 3 exports list
Coloured light, then obtain detection light and reference light through spectroscope 4；Reference light is for eliminating bias light interference, and detection light is through binary transflection
It penetrates 5 rear steering of mirror and enters sample cell 7 and be radiated in tested seawater, generate transmitted light, reflected fluorescent light；Reflected fluorescent light is through diffraction grating
It is formed after 9 and electric signal is become by electron multiplication CCD10 by the spectrum of wave arrangement, the fluorescence for collecting different excitation wavelengths generations can
Formed three-dimensional fluorescence spectrum, the spectrum with reference to background optical information by signal driving with processing system 1 realize algae red tide and
The measurement of Phytoplankton ＆ Suspension red tide and toxicity is completed in the inside self compensation of toxicity detection sensor.
Wherein, the process that Phytoplankton ＆ Suspension red tide and toxicity measurement are carried out using the sensor is as follows：
Step 1：Opening signal drives the ultraviolet light sent out with processing system 1, driving LED array 2, linear adjustable filter
Wave array 3 projects monochromatic ultraviolet light；
Step 2：Monochromatic ultraviolet light divides through spectroscope 4 for detection light and reference light, the detection reference light of detector 11；
Step 3：Detection light generates reflected fluorescent light, reflected fluorescent light after 5 rear steering of binary transflector mirror enters sample cell 7
The spectrum by wave arrangement is formed after the second collimator 8 and diffraction grating 9, and different excitation waves are collected by electron multiplication CCD10
The long fluorescence generated, forms three-dimensional fluorescence spectrum；
Step 5：Signal drives handles the signal obtained by electron multiplication CCD10 and detector 11 with processing system 1, and
With reference to bias light, the content detection of algae chlorophyll concentration and hemotoxin is completed, realizes the measurement to algae red tide and toxicity.
(3) advantageous effect
The Phytoplankton ＆ Suspension red tide and its toxicity detection optics home position sensing that above-mentioned technical proposal is provided, with the prior art
Compare, have the advantage that including：1. being detected based on spectrographic technique, addition chemical reagent is not had to；2. equipment is small and exquisite, integrated level is high,
Original position on-line measurement can be achieved；3. three-dimensional fluorescence spectrum information is combined with reference to background optical information, accuracy of detection is effectively promoted.
Description of the drawings
Fig. 1 is principle schematic of the embodiment of the present invention.
In figure, the driving of 1- signals and processing system；2-LED arrays；The linear adjustable filter arrays of 3-；4- spectroscopes；5- bis-
First transflector mirror；6- first collimators；7- sample cells；The second collimators of 8-；9- diffraction grating；10- electron multiplications CCD；11- light
Detector.
Specific implementation mode
To keep the purpose of the present invention, content and advantage clearer, with reference to the accompanying drawings and examples, to the present invention's
Specific implementation mode is described in further detail.
Referring to Fig.1 shown in, Phytoplankton ＆ Suspension red tide and its toxicity detection optics home position sensing of the present invention include light source module,
Reference light detection module, sample cell and scattering light detection module, reflected fluorescent light detection module and signal driving and processing module；Letter
Number driving with processing module driving light source module provide monochromatic ultraviolet light, reference light detection module for algae red tide and toxicity detection
Monochromatic ultraviolet light is divided into reference light and detection light, and reference light is detected, detectable signal is compensated for reference background light；
Detection light enters sample cell and scattering light detection module, irradiates seawater to be detected, the fluorescence being reflected back is detected via reflected fluorescent light
Module detects；Detection signal and detectable signal are sent to signal driving and processing module, realize algae red tide and toxicity detection
The measurement of Phytoplankton ＆ Suspension red tide and toxicity is completed in the inside self compensation of sensor.
Light-emitting device is LED array 2, the linear adjustable filter array of 2 output end of LED array connection in the light source module
3, it is monochrome by output ultraviolet light filter, excitation wavelength range is 400~600nm, launch wavelength ranging from 650~750nm.
The reference light detection module includes spectroscope 4 and optical detector 11, and spectroscope 4 is located at light source emitting light path
On, minute surface is in 45° angle with light source exit direction, and reflectivity is 10~30%；Monochromatic ultraviolet light is divided into vertical two by spectroscope 4
Beam, a branch of as detection light, a branch of as light is referred to, optical detector 11 is located in reference light light path (is in light source exit direction
90 ° of angles), detectable signal is compensated for reference background light.
The sample cell and scattering light detection module include binary transflector mirror 5, first collimator 6, sample cell 7, and two
First transflector mirror 6 is located on light source emitting light path after spectroscope 4, and minute surface is in 45° angle with light source direction of illumination, in its reflected light
First collimator 6, sample cell 7, the cloth between binary transflector mirror 5 and sample cell 7 of first collimator 6 are sequentially arranged in light path
It sets, ultraviolet light is converged in sample cell 7, seawater to be detected is held in sample cell 7.
The reflected fluorescent light detection module includes the second collimator 8, diffraction grating 9, electron multiplication CCD10, and second is accurate
Straight device 8 and diffraction grating 9 are located at 7 opposite side of first collimator 6 and sample cell, for collect be reflected back from sample cell 7 it is glimmering
Light, reflected fluorescent light are collimated through the second collimator 8, and diffraction grating 9 will be launched by the reflected fluorescent light of collimation by wave arrangement
Spectrum, electron multiplication CCD10 are located at the reflectance spectrum position of the formation of diffraction grating 9, the optical signal of fluorescence spectrum are transformed into
Electric signal.
The electric signal that reference optical signal, the reflected fluorescent light detection module of the reference light detection module detection are converted is equal
It is connected to signal driving and processing module 1 by signal wire, algae red tide and toxicity are realized with processing module 1 by signal driving
The measurement of Phytoplankton ＆ Suspension red tide and toxicity is completed in the inside self compensation of detection sensor.
The operation principle of inventive sensor is：The linear adjustable filter array of light that LED array 2 is sent out 3 exports monochrome
Light, then obtain detection light and reference light through spectroscope 4；Reference light is for eliminating bias light interference, and detection light is through binary transflector
5 rear steering of mirror enters sample cell 7 and is radiated in tested seawater, generates transmitted light, reflected fluorescent light；Reflected fluorescent light is through diffraction grating 9
It is formed afterwards and electric signal is become by electron multiplication CCD10 by the spectrum of wave arrangement, the fluorescence for collecting different excitation wavelengths generations can
Formed three-dimensional fluorescence spectrum, the spectrum with reference to background optical information by signal driving with processing system 1 realize algae red tide and
The measurement of Phytoplankton ＆ Suspension red tide and toxicity is completed in the inside self compensation of toxicity detection sensor.
The inside self compensation for realizing algae red tide and toxicity detection sensor with processing system 1 by signal driving, is completed
The process of Phytoplankton ＆ Suspension red tide and toxicity measurement is as follows：
Step 1：Opening signal drives the ultraviolet light sent out with processing system 1, driving LED array 2, linear adjustable filter
Wave array 3 projects monochromatic ultraviolet light；
Step 2：Emergent light divides through spectroscope 4 for detection light and reference light, the detection reference light of detector 11；
Step 3：Detection light generates reflected fluorescent light, reflected fluorescent light after 5 rear steering of binary transflector mirror enters sample cell 7
The spectrum by wave arrangement is formed after the second collimator 8 and diffraction grating 9, and different excitation waves are collected by electron multiplication CCD10
The long fluorescence generated, forms three-dimensional fluorescence spectrum；
Step 5：Signal drives handles the signal obtained by electron multiplication CCD10 and detector 11 with processing system 1, and
With reference to bias light, the content detection of algae chlorophyll concentration and hemotoxin is completed, realizes the measurement to algae red tide and toxicity.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, without departing from the technical principles of the invention, several improvement and deformations can also be made, these improvement and deformations
Also it should be regarded as protection scope of the present invention.
Claims (8)
1. a kind of Phytoplankton ＆ Suspension red tide and its toxicity detection optics home position sensing, which is characterized in that including：Light source module, ginseng
Examine light detection module, sample cell and scattering light detection module, reflected fluorescent light detection module and signal driving and processing module；Signal
Driving provides monochromatic ultraviolet light with processing module driving light source module for algae red tide and toxicity detection, and reference light detection module will
Monochromatic ultraviolet light is divided into reference light and detection light, and is detected to reference light, and detectable signal is compensated for reference background light；It visits
It surveys light and enters sample cell and scattering light detection module, irradiate seawater to be detected, the fluorescence being reflected back detects mould via reflected fluorescent light
Block detects；Detection signal and detectable signal are sent to signal driving and processing module, realize that algae red tide and toxicity detection pass
The measurement of Phytoplankton ＆ Suspension red tide and toxicity is completed in the inside self compensation of sensor.
2. Phytoplankton ＆ Suspension red tide as described in claim 1 and its toxicity detection optics home position sensing, which is characterized in that described
Light source module includes LED array (2) and adjustable filter array (3), the linear adjustable filter array of LED array (2) output end connection
(3), it is monochromatic ultraviolet light by output ultraviolet light filter.
3. Phytoplankton ＆ Suspension red tide as claimed in claim 2 and its toxicity detection optics home position sensing, which is characterized in that described
Reference light detection module includes spectroscope (4) and optical detector (11), and spectroscope (4) is located on monochromatic ultraviolet light emitting light path,
Minute surface is in 45° angle with monochromatic ultraviolet light exit direction, and for monochromatic ultraviolet light after spectroscope (4), the light beam of transmission is used as detection light,
The light beam of reflection, which is used as, refers to light, and optical detector (11) is located in reference light light path, and detectable signal is compensated for reference background light.
4. Phytoplankton ＆ Suspension red tide as claimed in claim 3 and its toxicity detection optics home position sensing, which is characterized in that described
Spectroscope (4) is 10~30% to the reflectivity of monochromatic ultraviolet light.
5. Phytoplankton ＆ Suspension red tide as claimed in claim 3 and its toxicity detection optics home position sensing, which is characterized in that described
Sample cell and scattering light detection module include binary transflector mirror (5), first collimator (6), sample cell (7), binary transflector
Mirror (6) is located on light source emitting light path after spectroscope (4), and minute surface is in 45° angle with light source direction of illumination, in its reflected light light path
On be sequentially arranged first collimator (6), sample cell (7), first collimator (6) binary transflector mirror (5) and sample cell (7) it
Between arrange, ultraviolet light is converged in sample cell (7), sample cell holds seawater to be detected in (7).
6. Phytoplankton ＆ Suspension red tide as claimed in claim 5 and its toxicity detection optics home position sensing, which is characterized in that described
Reflected fluorescent light detection module include the second collimator (8), diffraction grating (9), electron multiplication CCD (10), the second collimator (8) and
Diffraction grating (9) is located at first collimator (6) and sample cell (7) opposite side, for collect be reflected back from sample cell (7) it is glimmering
Light, reflected fluorescent light are collimated through the second collimator (8), and diffraction grating (9) will be launched by the reflected fluorescent light of collimation and be arranged by wavelength
The spectrum of row, electron multiplication CCD (10) are located at the reflectance spectrum position of diffraction grating (9) formation, and the light of fluorescence spectrum is believed
Number it is transformed into electric signal.
7. Phytoplankton ＆ Suspension red tide as claimed in claim 6 and its toxicity detection optics home position sensing, which is characterized in that described
The operation principle of sensor is：The linear adjustable filter array (3) of light that LED array (2) is sent out exports monochromatic light, then through light splitting
Mirror (4) obtains detection light and reference light；Reference light turns for eliminating bias light interference, detection light after binary transflector mirror (5)
It is radiated in tested seawater to sample cell (7) is entered, generates transmitted light, reflected fluorescent light；Reflected fluorescent light shape after diffraction grating (9)
Electric signal is become by electron multiplication CCD (10) at the spectrum by wave arrangement, the fluorescence for collecting different excitation wavelengths generations can shape
At three-dimensional fluorescence spectrum, the spectrum with reference to background optical information by signal driving and processing system (1) realize algae red tide and
The measurement of Phytoplankton ＆ Suspension red tide and toxicity is completed in the inside self compensation of toxicity detection sensor.
8. Phytoplankton ＆ Suspension red tide as claimed in claim 6 and its toxicity detection optics home position sensing, which is characterized in that utilize
The sensor carries out Phytoplankton ＆ Suspension red tide and the process of toxicity measurement is as follows：
Step 1：Opening signal drives the ultraviolet light sent out with processing system (1), driving LED array (2), linear adjustable filter
Wave array (3) projects monochromatic ultraviolet light；
Step 2：Monochromatic ultraviolet light is divided into detection light and reference light through spectroscope (4), and detector (11) detects reference light；
Step 3：Detection light generates reflected fluorescent light, reflected fluorescent light after binary transflector mirror (5) rear steering enters sample cell (7)
The spectrum by wave arrangement is formed after the second collimator (8) and diffraction grating (9), is collected by electron multiplication CCD (10) different
The fluorescence that excitation wavelength generates forms three-dimensional fluorescence spectrum；
Step 5：Signal drives handles the signal obtained by electron multiplication CCD (10) and detector (11) with processing system (1),
And bias light is referred to, the content detection of algae chlorophyll concentration and hemotoxin is completed, realizes the survey to algae red tide and toxicity
Amount.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109655110A (en) * | 2019-01-02 | 2019-04-19 | 南京大学 | Composite package LED light source and water monitoring device based on the light source |
| CN113218867A (en) * | 2021-05-17 | 2021-08-06 | 燕山大学 | Fixed bed frame of red tide algae recognition device |
| CN113933242A (en) * | 2021-09-16 | 2022-01-14 | 燕山大学 | Multi-source spectrum total organic carbon in-situ sensor optical path structure and application method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104316505A (en) * | 2014-11-14 | 2015-01-28 | 深圳市朗诚实业有限公司 | Structure and application of three-dimensional fluorescence standard spectrum library used for recognizing toxic-to-fish algae |
| CN106932373A (en) * | 2017-04-01 | 2017-07-07 | 燕山大学 | Total organic carbon optics home position sensing |
| CN208334198U (en) * | 2018-02-09 | 2019-01-04 | 秦皇岛红燕光电科技有限公司 | Phytoplankton & Suspension red tide and its toxicity detection optics home position sensing |
-
2018
- 2018-02-09 CN CN201810132036.3A patent/CN108279225A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104316505A (en) * | 2014-11-14 | 2015-01-28 | 深圳市朗诚实业有限公司 | Structure and application of three-dimensional fluorescence standard spectrum library used for recognizing toxic-to-fish algae |
| CN106932373A (en) * | 2017-04-01 | 2017-07-07 | 燕山大学 | Total organic carbon optics home position sensing |
| CN208334198U (en) * | 2018-02-09 | 2019-01-04 | 秦皇岛红燕光电科技有限公司 | Phytoplankton & Suspension red tide and its toxicity detection optics home position sensing |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109655110A (en) * | 2019-01-02 | 2019-04-19 | 南京大学 | Composite package LED light source and water monitoring device based on the light source |
| CN113218867A (en) * | 2021-05-17 | 2021-08-06 | 燕山大学 | Fixed bed frame of red tide algae recognition device |
| CN113933242A (en) * | 2021-09-16 | 2022-01-14 | 燕山大学 | Multi-source spectrum total organic carbon in-situ sensor optical path structure and application method thereof |
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