CN208334198U - Phytoplankton & Suspension red tide and its toxicity detection optics home position sensing - Google Patents
Phytoplankton & Suspension red tide and its toxicity detection optics home position sensing Download PDFInfo
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- CN208334198U CN208334198U CN201820230163.2U CN201820230163U CN208334198U CN 208334198 U CN208334198 U CN 208334198U CN 201820230163 U CN201820230163 U CN 201820230163U CN 208334198 U CN208334198 U CN 208334198U
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Abstract
The utility model discloses a kind of Phytoplankton & Suspension red tide and its toxicity detection optics home position sensings, comprising: light source module, reference light detection module, sample cell and scattering light detection module, reflected fluorescent light detection module and signal driving and processing module；Signal driving provides monochromatic ultraviolet light with processing module driving light source module for algae red tide and toxicity detection, and monochromatic ultraviolet light is divided into reference light and detection light by reference light detection module, and is detected to reference light, and detectable signal is compensated for reference background light；Detection light enters sample cell and scattering light detection module, irradiates seawater to be detected, the fluorescence being reflected back is detected via reflected fluorescent light detection module；Detection signal and detectable signal are sent to signal driving and processing module, realize the inside self compensation of algae red tide and toxicity detection sensor, complete the measurement of Phytoplankton & Suspension red tide and toxicity.The utility model device is small and exquisite, integrated level is high, it can be achieved that on-line measurement in situ, detection accuracy are high.
Description
Technical field
The utility model belongs to algae red tide and toxicity detection technical field, is related to a kind of sea based on three-dimensional fluorescence spectrum
Algae class red tide and its toxicity detection optics home position sensing.
Background technique
Red tide (harmful algal bloom, HAB), also known as red tide, are certain in seawater under certain environmental conditions
A little phytoplanktons, protozoan or bacterium burst proliferation or high aggregation and cause a kind of harmful ecology of water color-changing existing
As.Red tide can make the aquatic animals such as fish cause machine this is because red tide planktonic organism blocks the fish gill by very big harm sometimes
Tool obstacle, they are after death decomposed, and consume oxygen rapidly, and water oxygen is insufficient, and caused by secretion harmful substance etc..Fish toxicity
(Ichthyotoxic) red tide can produce a variety of noxious materials, cause fish and shellfish mortality in a short time.Fish toxicity
Red tide algae has been distributed widely in China coast, seriously polluted China's mariculture environment, restricts China coast seawater and supports
Grow the sustainable and healthy development of industry.
The detection method of Phytoplankton & Suspension red tide and toxicity mainly includes following three classes at present: first is that it is based on pigment spectroscopic methodology,
Second is that fluorescent spectrometry is based on, third is that using unicellular technique for taking is visualized online.Since the absorption spectrum of phytoplankton is easy
It is influenced by yellow substance and light protection pigment, to limit the further development of pigment spectroscopic methodology.With fluorescence in recent years
The progress of detection technique and image analyzing and processing technology, three-dimensional fluorescence spectrum technology and the unicellular technique for taking of visualization are in algae
Identification aspect is rapidly developed, and three-dimensional fluorescence spectrum technology is increasingly mature, has developed a variety of petroleum, more of being applied at present
The professional equipment of chlordiphenyl, bio-hormone and microalgae identification etc..Chinese patent (patent No. CN201410648240.2) is public
A kind of construction method in three-dimensional fluorescence standard spectrum library of fish toxicity algae for identification is opened, this method passes through fish toxicity algae
The research of hemotoxin and chlorophyll three-dimensional fluorescence spectrum under different growing stage and environmental factor regulation, by cluster side
Method establishes three-dimensional fluorescence standard spectrum library, for identifying fish toxicity algae and its toxicity size.
Utility model content
(1) technical problems to be solved
Technical problem to be solved by the utility model is: providing a kind of Phytoplankton & Suspension red tide and its toxicity detection optics is in situ
Sensor obtains algae chlorophyll concentration and hemotoxin three-dimensional fluorescence spectrum, and realization automatically determines and data processing, realizes sea
The automatic on-line in situ detection of algae class red tide and toxicity achievees the effect that rapid reaction, non-maintaining, autonomous operation.
(2) technical solution
In order to solve the above-mentioned technical problem, the utility model provides a kind of Phytoplankton & Suspension red tide and its toxicity detection optics is former
Level sensor comprising: light source module, reference light detection module, sample cell and scattering light detection module, reflected fluorescent light detect mould
Block and signal driving and processing module；Signal driving is provided with processing module driving light source module for algae red tide and toxicity detection
Monochromatic ultraviolet light, monochromatic ultraviolet light is divided into reference light and detection light by reference light detection module, and is detected to reference light, is visited
Signal is surveyed to compensate for reference background light；Detection light enters sample cell and scattering light detection module, irradiates seawater to be detected, reflects
The fluorescence returned is detected via reflected fluorescent light detection module；Detection signal and detectable signal are sent to signal driving and processing mould
Block realizes the inside self compensation of algae red tide and toxicity detection sensor, completes the measurement of Phytoplankton & Suspension red tide and toxicity.
Wherein, the light source module includes LED array 2 and adjustable filter array 3, and the connection of 2 output end of LED array linearly may be used
Filter array 3 is adjusted, output ultraviolet light is filtered as monochromatic ultraviolet light.
Wherein, the reference light detection module includes spectroscope 4 and optical detector 11, and spectroscope 4 is located at monochromatic ultraviolet light
On emitting light path, mirror surface and monochromatic ultraviolet light exit direction are in 45° angle, and monochromatic ultraviolet light after spectroscope 4, make by the light beam of transmission
To detect light, as reference light, optical detector 11 is located in reference light optical path the light beam of reflection, and detectable signal is used for reference background
Light compensation.
Wherein, the spectroscope 4 is 10~30% to the reflectivity of monochromatic ultraviolet light.
Wherein, the sample cell and scattering light detection module include binary transflector mirror 5, first collimator 6, sample cell 7,
Binary transflector mirror 6 is located on light source emitting light path after spectroscope 4, and mirror surface and light source direction of illumination are in 45° angle, in its reflection
First collimator 6, sample cell 7, the cloth between binary transflector mirror 5 and sample cell 7 of first collimator 6 are sequentially arranged in light optical path
It sets, ultraviolet light is converged in sample cell 7, seawater to be detected is held in sample cell 7.
Wherein, the reflected fluorescent light detection module includes the second collimator 8, diffraction grating 9, electron multiplication CCD10, and second
Collimator 8 and diffraction grating 9 be located at 7 opposite side of first collimator 6 and sample cell, is reflected back for collecting from sample cell 7
Fluorescence, reflected fluorescent light are collimated through the second collimator 8, and diffraction grating 9 will be launched by the reflected fluorescent light of collimation by wave arrangement
Spectrum, electron multiplication CCD10 is located at the reflectance spectrum position of the formation of diffraction grating 9, the optical signal of fluorescence spectrum changed
At electric signal.
Wherein, the working principle of the sensor are as follows: the linear adjustable filter array 3 of light that LED array 2 issues exports list
Coloured light, then detection light and reference light are obtained through spectroscope 4；Reference light detects light through binary transflection for eliminating bias light interference
It penetrates 5 rear steering of mirror and enters sample cell 7 and be radiated in tested seawater, generate transmitted light, reflected fluorescent light；Reflected fluorescent light is through diffraction grating
It is formed after 9 and electric signal is become by electron multiplication CCD10 by the spectrum of wave arrangement, the fluorescence for collecting different excitation wavelengths generations can
Formed three-dimensional fluorescence spectrum, the spectrum and with reference to background optical information by signal driving with processing system 1 realization algae red tide and
The measurement of Phytoplankton & Suspension red tide and toxicity is completed in the inside self compensation of toxicity detection sensor.
Wherein, the process for carrying out Phytoplankton & Suspension red tide and toxicity measurement using the sensor is as follows:
Step 1: opening signal driving and processing system 1, the ultraviolet light that driving LED array 2 issues, linear adjustable filter
Wave array 3 projects monochromatic ultraviolet light；
Step 2: monochromatic ultraviolet light divides through spectroscope 4 for detection light and reference light, the detection reference light of detector 11；
Step 3: detection light generates reflected fluorescent light, reflected fluorescent light after 5 rear steering of binary transflector mirror enters sample cell 7
The spectrum for pressing wave arrangement is formed after the second collimator 8 and diffraction grating 9, and different excitation waves are collected by electron multiplication CCD10
The long fluorescence generated, forms three-dimensional fluorescence spectrum；
Step 5: signal driving handles the signal obtained by electron multiplication CCD10 and detector 11 with processing system 1, and
With reference to bias light, the content detection of algae chlorophyll concentration and hemotoxin is completed, realizes the measurement to algae red tide and toxicity.
(3) beneficial effect
Phytoplankton & Suspension red tide provided by above-mentioned technical proposal and its toxicity detection optics home position sensing, with the prior art
It compares, has the advantages that 1. are detected based on spectrographic technique, do not have to addition chemical reagent；2. equipment is small and exquisite, integrated level is high,
Original position on-line measurement can be achieved；3. three-dimensional fluorescence spectrum information and reference background optical information are combined, effectively promotion detection accuracy.
Detailed description of the invention
Fig. 1 is the utility model embodiment schematic illustration.
In figure, the driving of 1- signal and processing system；2-LED array；The linear adjustable filter array of 3-；4- spectroscope；5- bis-
First transflector mirror；6- first collimator；7- sample cell；The second collimator of 8-；9- diffraction grating；10- electron multiplication CCD；11- light
Detector.
Specific embodiment
To keep the purpose of this utility model, content and advantage clearer, with reference to the accompanying drawings and examples, to this reality
It is described in further detail with novel specific embodiment.
Shown in referring to Fig.1, the utility model Phytoplankton & Suspension red tide and its toxicity detection optics home position sensing include light source
Module, reference light detection module, sample cell and scattering light detection module, reflected fluorescent light detection module and signal driving and processing mould
Block；Signal driving provides monochromatic ultraviolet light, reference light inspection with processing module driving light source module for algae red tide and toxicity detection
It surveys module and monochromatic ultraviolet light is divided into reference light and detection light, and reference light is detected, detectable signal is used for reference background
Light compensation；Detection light enters sample cell and scattering light detection module, irradiates seawater to be detected, and the fluorescence being reflected back is glimmering via reflecting
The detection of light detection module；Detection signal and detectable signal are sent to signal driving and processing module, realize algae red tide and poison
Property detection sensor inside self compensation, complete the measurement of Phytoplankton & Suspension red tide and toxicity.
Light emitting device is LED array 2 in the light source module, and 2 output end of LED array connects linear adjustable filter array
3, it is monochrome by output ultraviolet light filter, excitation wavelength range is 400~600nm, and launch wavelength range is 650~750nm.
The reference light detection module includes spectroscope 4 and optical detector 11, and spectroscope 4 is located at light source emitting light path
On, mirror surface and light source exit direction are in 45° angle, and reflectivity is 10~30%；Monochromatic ultraviolet light is divided into vertical two by spectroscope 4
Beam, a branch of as detection light, a branch of to be used as reference light, optical detector 11 is located in reference light optical path (is in light source exit direction
90 ° of angles), detectable signal is compensated for reference background light.
The sample cell and scattering light detection module include binary transflector mirror 5, first collimator 6, sample cell 7, and two
First transflector mirror 6 is located on light source emitting light path after spectroscope 4, and mirror surface and light source direction of illumination are in 45° angle, in its reflected light
First collimator 6, sample cell 7, the cloth between binary transflector mirror 5 and sample cell 7 of first collimator 6 are sequentially arranged in optical path
It sets, ultraviolet light is converged in sample cell 7, seawater to be detected is held in sample cell 7.
The reflected fluorescent light detection module includes the second collimator 8, diffraction grating 9, electron multiplication CCD10, and second is quasi-
Straight device 8 and diffraction grating 9 are located at 7 opposite side of first collimator 6 and sample cell, for collect be reflected back from sample cell 7 it is glimmering
Light, reflected fluorescent light are collimated through the second collimator 8, and diffraction grating 9 will be launched by the reflected fluorescent light of collimation by wave arrangement
Spectrum, electron multiplication CCD10 are located at the reflectance spectrum position of the formation of diffraction grating 9, the optical signal of fluorescence spectrum are transformed into
Electric signal.
The electric signal that reference optical signal, the reflected fluorescent light detection module of the reference light detection module detection are converted is equal
It is connected to signal driving and processing module 1 by signal wire, algae red tide and toxicity are realized with processing module 1 by signal driving
The measurement of Phytoplankton & Suspension red tide and toxicity is completed in the inside self compensation of detection sensor.
The working principle of the utility model sensor are as follows: the linear adjustable filter array 3 of the light that LED array 2 issues exports
Monochromatic light, then detection light and reference light are obtained through spectroscope 4；For reference light for eliminating bias light interference, detection light is saturating through binary
5 rear steering of reflecting mirror enters sample cell 7 and is radiated in tested seawater, generates transmitted light, reflected fluorescent light；Reflected fluorescent light is through diffraction light
It is formed after grid 9 and electric signal is become by electron multiplication CCD10 by the spectrum of wave arrangement, collect the fluorescence that different excitation wavelengths generate
Three-dimensional fluorescence spectrum can be formed, the spectrum and reference background optical information pass through signal driving and realize algae red tide with processing system 1
And the inside self compensation of toxicity detection sensor, complete the measurement of Phytoplankton & Suspension red tide and toxicity.
The inside self compensation for realizing algae red tide and toxicity detection sensor with processing system 1 by signal driving, is completed
The process of Phytoplankton & Suspension red tide and toxicity measurement is as follows:
Step 1: opening signal driving and processing system 1, the ultraviolet light that driving LED array 2 issues, linear adjustable filter
Wave array 3 projects monochromatic ultraviolet light；
Step 2: emergent light divides through spectroscope 4 for detection light and reference light, the detection reference light of detector 11；
Step 3: detection light generates reflected fluorescent light, reflected fluorescent light after 5 rear steering of binary transflector mirror enters sample cell 7
The spectrum for pressing wave arrangement is formed after the second collimator 8 and diffraction grating 9, and different excitation waves are collected by electron multiplication CCD10
The long fluorescence generated, forms three-dimensional fluorescence spectrum；
Step 5: signal driving handles the signal obtained by electron multiplication CCD10 and detector 11 with processing system 1, and
With reference to bias light, the content detection of algae chlorophyll concentration and hemotoxin is completed, realizes the measurement to algae red tide and toxicity.
The above is only the preferred embodiment of the utility model, it is noted that for the common skill of the art
For art personnel, without deviating from the technical principle of the utility model, several improvement and deformations can also be made, these change
It also should be regarded as the protection scope of the utility model into deformation.
Claims (7)
1. a kind of Phytoplankton & Suspension red tide and its toxicity detection optics home position sensing characterized by comprising light source module, ginseng
Examine light detection module, sample cell and scattering light detection module, reflected fluorescent light detection module and signal driving and processing module；Signal
Driving provides monochromatic ultraviolet light with processing module driving light source module for algae red tide and toxicity detection, and reference light detection module will
Monochromatic ultraviolet light is divided into reference light and detection light, and detects to reference light, and detectable signal is compensated for reference background light；It visits
It surveys light and enters sample cell and scattering light detection module, irradiate seawater to be detected, the fluorescence being reflected back detects mould via reflected fluorescent light
Block detection；Detection signal and detectable signal are sent to signal driving and processing module, realize that algae red tide and toxicity detection pass
The measurement of Phytoplankton & Suspension red tide and toxicity is completed in the inside self compensation of sensor.
2. Phytoplankton & Suspension red tide as described in claim 1 and its toxicity detection optics home position sensing, which is characterized in that described
Light source module includes LED array (2) and adjustable filter array (3), and LED array (2) output end connects linear adjustable filter array
(3), output ultraviolet light is filtered as monochromatic ultraviolet light.
3. Phytoplankton & Suspension red tide as claimed in claim 2 and its toxicity detection optics home position sensing, which is characterized in that described
Reference light detection module includes spectroscope (4) and optical detector (11), and spectroscope (4) is located on monochromatic ultraviolet light emitting light path,
Mirror surface and monochromatic ultraviolet light exit direction are in 45° angle, and for monochromatic ultraviolet light after spectroscope (4), the light beam of transmission is used as detection light,
The light beam of reflection is located in reference light optical path as reference light, optical detector (11), and detectable signal is compensated for reference background light.
4. Phytoplankton & Suspension red tide as claimed in claim 3 and its toxicity detection optics home position sensing, which is characterized in that described
Spectroscope (4) is 10~30% to the reflectivity of monochromatic ultraviolet light.
5. Phytoplankton & Suspension red tide as claimed in claim 3 and its toxicity detection optics home position sensing, which is characterized in that described
Sample cell and scattering light detection module include binary transflector mirror (5), first collimator (6), sample cell (7), binary transflector
Mirror (5) is located on light source emitting light path after spectroscope (4), and mirror surface and light source direction of illumination are in 45° angle, in its reflected light optical path
On be sequentially arranged first collimator (6), sample cell (7), first collimator (6) is in binary transflector mirror (5) and sample cell (7)
Between arrange, ultraviolet light is converged in sample cell (7), sample cell holds seawater to be detected in (7).
6. Phytoplankton & Suspension red tide as claimed in claim 5 and its toxicity detection optics home position sensing, which is characterized in that described
Reflected fluorescent light detection module include the second collimator (8), diffraction grating (9), electron multiplication CCD (10), the second collimator (8) and
Diffraction grating (9) is located at first collimator (6) and sample cell (7) opposite side, for collect be reflected back from sample cell (7) it is glimmering
Light, reflected fluorescent light are collimated through the second collimator (8), and diffraction grating (9) will be launched by the reflected fluorescent light of collimation and arrange by wavelength
The spectrum of column, electron multiplication CCD (10) are located at the reflectance spectrum position of diffraction grating (9) formation, and the light of fluorescence spectrum is believed
Number it is transformed into electric signal.
7. Phytoplankton & Suspension red tide as claimed in claim 6 and its toxicity detection optics home position sensing, which is characterized in that described
The working principle of sensor are as follows: the linear adjustable filter array (3) of the light that LED array (2) issues exports monochromatic light, then through being divided
Mirror (4) obtains detection light and reference light；Reference light turns after binary transflector mirror (5) for eliminating bias light interference, detection light
It is radiated in tested seawater into sample cell (7), generates transmitted light, reflected fluorescent light；Reflected fluorescent light shape after diffraction grating (9)
Electric signal is become by electron multiplication CCD (10) at the spectrum by wave arrangement, the fluorescence for collecting different excitation wavelengths generations can shape
At three-dimensional fluorescence spectrum, the spectrum and with reference to background optical information by signal driving and processing system (1) realization algae red tide and
The measurement of Phytoplankton & Suspension red tide and toxicity is completed in the inside self compensation of toxicity detection sensor.
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| CN108279225A (en) * | 2018-02-09 | 2018-07-13 | 秦皇岛红燕光电科技有限公司 | Phytoplankton ＆ Suspension red tide and its toxicity detection optics home position sensing |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108279225A (en) * | 2018-02-09 | 2018-07-13 | 秦皇岛红燕光电科技有限公司 | Phytoplankton ＆ Suspension red tide and its toxicity detection optics home position sensing |
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